Digital Commons Network^™

Expression Analyses Of Hippocampal And Cortical Proteins In A Rat Model For Alzheimer’S Disease, Rangon Islam

Theses and Dissertations

Currently, Alzheimer’s disease (AD) has no cure. Using a rat AD model, we identified aberrantly expressed proteins during pre-pathology as potential biomarkers. The expression of certain biomarkers was reversed by diazoxide, a repurposed hypertension drug. These results suggest that drug repurposing at an early stage of AD has therapeutic potential.

The Effects Of Activity-Based Anorexia On The Rewarding Properties Of Methamphetamine And Wheel Running, Rachael M. Langa

Theses and Dissertations

An activity-based anorexia (ABA) paradigm in adolescent female mice was used to explore whether anorexia affects circuits underlying reward. The ABA paradigm significantly enhanced methamphetamine-induced conditioned place preference (CPP), but not wheel-induced CPP. These results indicate that the ABA paradigm enhances the rewarding properties of methamphetamine, but not wheel running.

Do Bats Forage At Different Heights When In The Presence Of Other Species?, Benjamin L. Wagenberg

Theses and Dissertations

Acoustic monitoring was used to examine whether bats forage at different heights in the presence of other species. The data indicate that the presence of other species does affect the foraging height of bats. This data can be used to increase effectiveness of other identification methods using acoustic monitoring.

A Comparative Assessment Of Working And Pet Dog Performance During A Visual Discrimination Reversal Learning Task, Laisuna Yu

Theses and Dissertations

Dogs play an increasingly important role in human society as companions but also in the working sector. The working dog industry is currently struggling to meet the high demands for working dogs across all sectors with only approximately half the dogs acquired reaching their intended careers. Current behavior and temperament assessments are lacking in standardization and objectivity when identifying successful working dogs, which has prompted the industry to re-evaluate the methods used when selecting dogs. Behavioral cognitive testing, including reversal learning, has proven to be a beneficial tool in assessing physical cognition in pet dogs and, more recently, in working …

Using Object-Choice Tasks To Investigate Sensory Perception In Sunda Pangolins (Manis Javanica), Joshua Dipaola

Theses and Dissertations

Pangolins are one of the most heavily poached, yet least understood mammals in the world. In this study, we used an object-choice task to assess the ecological relevance and use of sensory information in Sunda pangolin foraging behavior. This is the first controlled experiment on pangolin behavior to our knowledge.

Laser Diodes In Photoacoustic Flow Cytometry For Detection Of Particles And Cells, Hind Jasim Jawad

Theses and Dissertations

Photoacoustic (PA) flow cytometry (PAFC) is a powerful diagnostic method that has vast potential in clinical diagnosis of cancer, infections, and cardiovascular disorders by providing highly sensitive detection and enumeration of individual circulating cells or particles associated with presence and progression of a disease. This promising diagnostic tool commonly uses solid-state lasers as the illuminating light sources. However, these lasers are usually expensive and bulky, limiting their usage in many clinical applications. Low-cost, portable, high-power pulsed diode lasers can be used as potential alternative sources for PAFC due to their high efficiency, and availability in a wide spectral range of …

Biomaterials For Bone Regeneration: Tunable Structural And Mechanical Characteristics, Anwer Mhanawee

Theses and Dissertations

Tissue engineering is a promising alternative treatment for damaged tissues. In bone regeneration, bone graft replacements can be made from different biomaterials (basically ceramic and polymer) and used for different treatment purposes. In the first section of this dissertation, the Compression test results showed that its modulus of elasticity depends on its porosity. Also, we developed a testing method to measure the swelling force of the material during expansion due to liquid absorption. The maximum swelling force was found to be proportional to the material modulus. In the second part, we successfully design, fabricate and characterize a novel expandable bone …

Numerical Simulation To The Fitzhugh-Nagumo Model With Strong Reaction, Hongsong Feng

Theses and Dissertations

The Fitzhugh-Nagumo model is a mathematical model derived from the simulation of propagating pulses in multicellular organisms. Since its creation, this model has drawn great attentions from academics and industry. To better understand the properties underlying this system, suitable numerical methods are needed to study it. In this thesis, numerical methods including the finite difference method, the finite element method, and the least-squares finite element method are applied to approximate its traveling wave solutions. In particular, since the FitzHugh-Nagumo model with strong reaction has a significant role in application, appropriate numerical scheme is designed to study it. Consistency and stability …

The Maze Of Personality: Latency And Electric Organ Discharge In A Mormyrid Fish, Gnathonemus Petersii Gunther 1862 (Mormyridae, Teleostei), Abbey C. Lipe

Theses and Dissertations

Personality is individual differences in behavior, consistent across contexts. Among Gnathonemus petersii we investigated, we hypothesized that fish could be grouped by: (1) slow/fast maze performance, (2) low/high electric frequencies, (3) correlated latency and frequency. Our first two hypothesis were not supported. Our third hypothesis was partially supported.

Circulating Micrornas In Neuromuscular Disorders, Nahla Mousa

Theses and Dissertations

Identification of the mutations and genetic causes of the muscular diseases is performed for the muscular dystrophies, atrophies and other genetic diseases using current molecular high throughput techniques that precisely allocate the defected genes and detect the aberration in the expressed proteins responsible for any phenotype. For example, more than 2000 mutations in the DMD gene were identified in Duchenne Muscular Dystrophy patients or the milder form of the disease Becker Muscular Dystrophy. Detection of different mutation in the same gene has critical role as biomarkers in disease diagnosis, since the severity of the symptoms is usually associated with the …

Fraud Detection Using Data-Driven Approach, Arianit Mehana

Theses and Dissertations

The extensive use of internet is continuously drifting businesses to incorporate their services in the online environment. One of the first spectrums to embrace this evolution was the banking sector. In fact, the first known online banking service came in 1980. It was deployed from a community bank located in Knoxville, called the United American Bank. Since then, internet banking has been offering ease and efficiency to costumers in completing their daily banking tasks.

The ever increasing use of internet banking and the large number of online transactions, increased fraudulent behaviour also. As if fraud increase wasn’t enough, the massive …

Mechanical And Thermal Behavior Of Multiscale Bi-Nano-Composites Using Experiments And Machine Learning Predictions, Vahid Daghigh

Theses and Dissertations

The mechanical and thermal properties of natural short latania fiber (SLF)-reinforced poly(propylene)/ethylene-propylene-diene-monomer (SLF/PP/EPDM) bio-composites reinforced with nano-clays (NCs), pistachio shell powders (PSPs), and/or date seed particles (DSPs) were studied using experiments and machine learning (ML) predictions. This dissertation embraces three related investigations: (1) an assessment of maleated polypropylene (MAPP) coupling agent on mechanical and thermal behavior of SLF/PP/EPDM composites, (2) heat deflection temperature (HDT) of bio-nano-composites using experiments and ML predictions, and (3) fracture toughness ML predictions of short fiber, nano- and micro-particle reinforced composites. The first project (Chapter 2) investigates the influence of MAPP on tensile, bending, Charpy impact …

Defining The Mechanical Characteristics Of Porcine Brain Tissue Subject To Cyclic, Compressive Loading, Kali Sebastian

Theses and Dissertations

In recent years, repetitive traumatic brain injuries have been linked to the progressive neurodegenerative disorder termed chronic traumatic encephalopathy. However, the mechanical characteristics of brain tissue exposed to repetitive loading still lack understanding. This research evaluated the response of porcine brain tissue undergoing cyclic, compressive loading in reference to three impact parameters: cycle number (N25, N50, N100, N150, and N200), strain level (15, 30, and 40%), and strain rate (0.00625, 0.025, 0.10, and 1.0/s). Following mechanical testing, tissue samples were processed for hematoxylin and eosin (H&E) staining. Stress values, hysteresis energy, and decreases in hysteresis energy for all parameters were …

Anomaly Detection And Classification Of Sparse Gamma-Ray Spectra Using Machine Learning Algorithms For Depleted Uranium Remediation, Austin Finney

Theses and Dissertations

The quality of the spectral data collected by radiological survey systems depends on many factors including the survey environment, configuration of the system and its detectors, and the radionuclides in question. Algorithms in the field of machine learning have the potential to classify data that would be difficult and time-intensive for a human to analyze. Depleted and natural uranium spectra are of particular interest due to known contamination at domestic sites and world-wide. Several machine learning classifiers were developed with data collected from laboratory experiments. This thesis demonstrates the potential of machine learning algorithms to discriminate gamma-ray emitting sources using …

Characteristics And Spatial Heterogeneity Of Prescribed Fire Behavior In North Dakota Grasslands, Megan Elizabeth Zopfi

Theses and Dissertations

Fire is a critical physical and chemical process required to sustain many grassland ecosystems. In North America, observations of grassland fire behavior in warm-season, southern grasslands are commonly used in fire behavior modeling efforts across the Great Plains. However, grasslands of the northern Great Plains contain a greater component of cool-season vegetation that may generate different fire behavior. To further our understanding of prescribed fire behavior in North Dakota grasslands, we quantified fuel, weather, and fire behavior characteristics associated with 27 prescribed fires conducted across three sites in North Dakota. We sampled 27 points on each fire arranged into a …

Approach And Avoidance Coping Humor Motives And Their Relation To Trauma Exposure And Physical Health, Andrea D. Dorbu

Theses and Dissertations

The present study investigated approach-avoidance motives for humor use and their association with physical health symptoms linked to childhood trauma exposure and adult relationship trauma exposure among military-affiliated people and civilians (N=100). Results indicated that approach-avoidance motives for humor use were not associated with study variables. However, adult relationship trauma exposure had a significant positive association with physical health symptoms among both groups. In addition, childhood trauma exposure was significantly positively associated with adult relationship trauma exposure.

Laying-Sequence Variation In Yolk Carotenoids Of Eastern Bluebirds, Jessica Barkhouse

Theses and Dissertations

Carotenoids are naturally occurring, fat-soluble pigments that play an important role in the embryonic development of songbirds. Female songbirds use different strategies to allocate these important maternal resources to their eggs. Little research has been done on whether North American songbirds exhibit laying sequence patterns in their allocation of carotenoids. We studied the laying-sequence variation in yolk carotenoids and egg metrics of nine full clutches of Eastern Bluebirds (Sialia sialis) in Columbus, Georgia. I predicted that Eastern Bluebirds would exhibit a brood reduction strategy, with the first-laid eggs containing a higher concentration of carotenoids than the last-laid eggs. …

Estimating Taxonomic Diversity Using Centrum Growth Profiles And Stinger Morphology Of 36 Million Year Old Stingrays From North Dakota, Persia S. Tillman

Theses and Dissertations

Stingrays are a diverse and popular group of vertebrates; however, nothing is known about the relationships between growth biology and climate change. Freshwater stingrays once inhabit the United States and Canada during very warm times in the geologic record. No stingray material has been recorded from the northern part of the United States for the last 33 million years. The Earth’s climate cooled from 50 to 33 million year ago when many warm adapted organisms were relegated to warmer, southern latitudes in North America. Today, freshwater stingrays only inhabit subtropical and tropical environments. We are interested in the freshwater stingrays …

Earliest Known Material Of Amia, Bowfin, From The Sentinel Butte Formation (Paleocene), Medora, North Dakota, Abby Grace Moore

Theses and Dissertations

Amia calva is an icon in the field of comparative osteology, yet we have a poor understanding of the evolution of the genus because many fossil amiid bones have gone unidentified. Here we identify new material of the genus, Amia, with evidence of two unidentified species. Previously, the oldest known material identifiable as Amia cf. A. pattersoni, was a specimen from the Paleocene epoch of Alberta, Canada approximately 58 million years in age. Fossils of the two unidentified species of Amia were found in the Sentinel Butte Formation, a geologic formation of Paleocene age (~ 60 million years …

Recombinant Expression Of Preptin Analogs For Alanine Scanning Mutagenesis Of Residues 27-29, Tehgan N. Anguilm

Theses and Dissertations

The novel peptide preptin consists of 34 amino acid residues corresponding to Asp⁶⁹-Leu¹⁰² of proinsulin-like growth factor E-peptide. Preptin has been isolated from pancreatic beta cells, and it has been found to exhibit both metabolic and mitogenic activities. This makes preptin an attractive candidate to treat insulin-independent diabetes and osteoporosis. Alanine scanning mutagenesis was utilized to substitute an alanine for residues Trp²⁷, Arg²⁸, and Gln²⁹ of preptin in order to ultimately study the structure-activity relationship of these residues to preptin’s metabolic activity.

Knowledge, Trait Mindfulness And Perceived Discrimination As It Relates To Coping Self-Efficacy In African American Women, Collins D. Nelson

Theses and Dissertations

This is a cross-sectional study that investigated the relationship between preconception care knowledge, trait mindfulness, perceived discrimination, and coping self-efficacy. It addressed the lack of research into the effects of pregnancy-related distress on maternal mortality in African American women. There were 117 participants ages 18 -30 whose data was collected through an online survey. The results suggest that greater preconception care knowledge predicts greater pregnancy-related distress coping self-efficacy. Trait mindfulness also predicted pregnancy-related distress coping self-efficacy. Perceived discrimination did not predict coping self- efficacy in a negative direction as expected.

Implementing Writing In Content Areas: Teachers’ Perceptions As Writing Instructors, Matthew Davis Shemwell

Theses and Dissertations

Teachers across content areas have a shared responsibility to incorporate writing instruction into the curriculum; however, analysis of needs assessment survey data collected during Striving Readers Comprehensive Literacy grant application in 2014 revealed that the faculty of the participating high school did not embrace writing instruction responsibility. The purpose of the study was to investigate teachers’ perceptions, confidence, and use of writing instructional strategies throughout content areas at the conclusion of implementation of the five-year grant. The researcher conducted an explanatory sequential mixed methods design that utilized a survey, interviews, and lesson plan document analysis. The survey sample consisted of …

Evaluating Seed Blended Refugia In Field Corn In The Southern U. S., Tyler Breck Towles

Theses and Dissertations

Helicoverpa zea (Boddie), a pest of cotton that also occurs in field corn, is commonly controlled through the use of foliar-applied insecticides or transgenic crops expressing Bt genes. To prevent the selection of resistant populations, refuge systems have been implemented into the agroecosystem. Historically, structured refuge compliance among growers has been low, leading to the commercialization of seed blended refugia. To test the viability of seed blended refugia in the southern U.S., field studies were conducted in Mississippi and Georgia during the 2016, 2017, and 2018 growing seasons. To quantify adult H. zea emergence from structured and seed blended refuge …

Effects Of Dietary Lysine Restriction On Nutrient Metabolism And Muscle Gene Expression In Young Growing Pigs, Md Shamimul Hasan

Theses and Dissertations

The objectives of this project were to investigate the effects of dietary lysine restriction on (1) the plasma concentrations of selected metabolites, free AAs, and three growth-related hormones, (2) the gene expression profile in longissimus dorsi muscle growing pigs. Twelve individually penned young barrows (Yorkshire × Landrace; 22.6 ± 2.04 kg) were randomly assigned to two dietary treatments (a lysine-deficient, LDD, and a lysineequate, LAD) diets, respectively. Pigs had ad libitum access to water and their respective diets for 8 weeks, and the ADG, ADFI, and G:F were determined. At the end of the trial, jugular vein blood was collected, …

Biophysical Characterization Of Protein-Nanoparticle Interactions, Yasiru Randika Perera

Theses and Dissertations

Nanoparticles (NPs) have become a key tool in medicine and biotechnology; as drug delivery systems, biosensors, and diagnostic devices. However, the mechanism of biocorona formation on nanoparticle surfaces and their impact on drug delivery remains speculative. Nevertheless, functionalized nanoparticles have demonstrated major success in medical applications; having been shown to effectively treat disease. The mechanistic details of protein behavior on nanoparticle surfaces remain poorly understood to date; due to difficulty in determining the orientation and structure of protein on NPs. Furthermore, surface crowding, orientation, and degree of disorder have been shown to perturb the efficacy of protein on NPs; dramatically …

Building A Predictor Model Of Variables For Students Currently In A Counseling Program And The Impact Those Variables Have On Their Levels Of Empathy, Susan Hope Tipton

Theses and Dissertations

The purpose of the study is to investigate a variety of character traits, stressors or traumatic event variables in order to develop a possible predictor model for individuals that seek out to become a student in a counseling program and how those variables relate to their level of empathy. The purpose of using a multiple linear regression analysis is to help determine if a linear relationship exist between the dependent variable and a set of independent variables. The multiple linear regression analyzes the data to determine if the residuals are homoscedastic and approximately rectangular-shaped. A multiple linear regression allows the …

Assessment Of Coastal Watershed Erosion Potential Using Geographic Information Systems And Expert Input For Decision Support, John H. Cartwright

Theses and Dissertations

Sediment is a major impairment in many streams and rivers in the drainage basins along the northern Gulf of Mexico. The use of geospatial technologies improves assessment and decision making for the management of environmental resources and conditions for coastal watersheds. This research focuses on the development of a conceptual qualitative model enhanced with expert input for the assessment of soil erosion potential in coastal watersheds. The conceptual model is built upon five layers (slope, precipitation, soil brightness or exposure, Kactor, and stream density) like those in a standard numerical soil loss model such as the Revised Universal Soil Loss …

Agronomic And Physiological Responses Of Resistant And Susceptible Cotton Genotypes To Reniform Nematode Severity And Soil Nitrogen, Bhupinder Singh

Theses and Dissertations

Rotylenchulus reniformis (RN) nematode infection has been identified as a major biotic factor limiting cotton yield (~8%) in Mississippi. Cotton researchers are striving to develop resistance to RN and develop management practices to mitigate the problem. Reniform nematode and soil nitrogen (N) directly affect cotton growth and physiology, however, responses to RN and N may vary across a wide genetic background. The overall objective of this research was to determine the role that host plant resistance to reniform nematode and soil nitrogen plays in cotton growth, physiology, and yield and controlling RN populations. In experiment 1, the effect of RN …

"Swamp Thing: Alligators, Symbolism, And The Meaning Of Animals In The American South, Nathan Drake

Theses and Dissertations

Humans form lasting and unique relationships with the natural world and, by extension, the organisms and animals who have for millennia carved out niche environments. Scholars and general observers agree—at least in principle—that human beings have actively shaped (for better and for worse) the habitats, behaviors, and population of the Earth’s creatures. In turn, those spaces and animals have influenced not only how humans think of the natural world, but also of humanity itself. Animals, in other words, help humans understand themselves.1 This dissertation is a history of the American Alligator. A study of human interactions with alligators can reveal …

Modeling Frameworks To Evaluate Energy Autarky Of Wastewater Treatment Systems, Gideon Sarpong

Theses and Dissertations

This research demonstrates the use of two novel methodologies to evaluate energy autarky status of wastewater treatment plants (WWTPs) in two steps. Step I (analysis 1 and 2) focuses on overall energy performance evaluation of a conventional activated sludge process (CAS) using a quantitative mass balance model. Step II involves development of a dynamic model that simulates a future wastewater resource recovery facility (WRRF). The step I (analysis 1) focused on small WWTPs with treatment capacities less than 5 MGD. The results revealed that a CAS process can achieve energy autarky or energy-positive status when old technology equipment is replaced …