Digital Commons Network^™

Outcome Of Endodontic Microsurgery: Success And Survival Rates At Virginia Commonwealth University, Abdullah Alawadhi

Theses and Dissertations

Introduction: Endodontic microsurgery treatment is an option for teeth with apical periodontitis and may be indicated for teeth with previous endodontic treatment that have not healed, teeth with canal obstructions which would impede a non-surgical approach, and where a biopsy is needed. The purpose of this study is to evaluate healing after endodontic microsurgery in 2-dimensional PA radiographs and 3 dimensional CBCT imaging. Methods: Patient records were pooled from graduate resident cases completed at Virginia Commonwealth University in the Graduate Endodontic Clinic between the years 2010 to 2020. Cases were identified using the CDT codes and a key word search …

Adaptations In Orthodontics For Current And Future Covid-19 Best Practices, Jordan Lamb

Theses and Dissertations

Purpose: To determine what adaptations orthodontists made in their practices during the COVID-19 pandemic in response to safety recommendations and to determine which of these adaptations orthodontists plan to continue to implement after resolution of the COVID-19 pandemic.

Methods: An original 34-question survey was sent by mail to a randomized and geographically proportionate selection of actively practicing orthodontic specialists (N = 1000). Questions asked orthodontists about the changes they made during the pandemic in four overarching categories (infection control, social distancing, appliance use, and tele-orthodontics) and whether they anticipated keeping those changes post-pandemic. The data were compared between categorical responses …

The Effect Of Er:Yag, Nd:Yag And Co2 Laser Combined With Hydrogen Peroxide, Sodium Hypochlorite, Chlorohexidine Or Fluoride On Reducing Oral Bacterial Count Implicated In Root Caries, Nitya Reddy

Theses and Dissertations

Purpose: Lasers have been used for treatment of dentinal hypersensitivity and for various bacterial reduction indications in periodontology. Their effectiveness in killing oral bacteria is not well known. The compounding effect of the combination of a laser treatment and adjunct antimicrobial agent use on bacterial viability is still evolving. The purpose of this in vitro study was to evaluate the effect of three lasers commonly used in dentistry in conjunction with chlorhexidine (CHX), hydrogen peroxide (H₂O₂), sodium hypochlorite (NaOCl), or sodium fluoride (NaF) on viability of oral bacteria associated with root caries.

Methods: Three …

A Study On Developing Novel Methods For Relation Extraction, Darshini Mahendran

Theses and Dissertations

Relation Extraction (RE) is a task of Natural Language Processing (NLP) to detect and classify the relations between two entities. Relation extraction in the biomedical and scientific literature domain is challenging as text can contain multiple pairs of entities in the same instance. During the course of this research, we developed an RE framework (RelEx), which consists of five main RE paradigms: rule-based, machine learning-based, Convolutional Neural Network (CNN)-based, Bidirectional Encoder Representations from Transformers (BERT)-based, and Graph Convolutional Networks (GCNs)-based approaches. RelEx's rule-based approach uses co-location information of the entities to determine whether a relation exists between a selected entity …

Characteristics Of Posterior Permanent Teeth With A Periradicular Diagnosis Of Symptomatic Apical Periodontitis: A Retrospective Study, Christina A. Martin

Theses and Dissertations

The diagnosis of apical periodontitis is based on radiographic manifestations and the results of the various clinical tests that can be performed as part of a routine dental examination. Apical periodontitis producing clinical symptoms involving a painful response to biting and/or percussion or palpation is given the diagnosis of symptomatic apical periodontitis (SAP). This study aimed to determine associations of SAP with gender, age, radiographic findings, diagnostic testing, pulpal diagnosis, and location of the tooth in the arch. Data for this study were obtained from patients of record treated at Virginia Commonwealth University School of Dentistry in the Department of …

Budget Impact Analysis Of Upadacitinib For The Management Of Moderate-To-Severe Atopic Dermatitis In Patients Treated With Systemic Therapies In The United States, Haya O. Alobaid

Theses and Dissertations

Objective: This study evaluated the budget impact of introducing upadacitinib for patients with uncontrolled moderate-to-severe atopic dermatitis (AD) from a United States (U.S.) private payer perspective.

Methods: The model estimated costs before and after the adoption of upadacitinib for a hypothetical one million covered lives over 3 years. The model included immunosuppressant agents and dupilumab. Market uptake was assumed to be 2% per year. Treatments incur a cost for drug acquisition, and the costs associated with drug administration, laboratory testing, and clinic visits. The model calculated the impact on the budget in 2022 U.S. dollars. Various assumptions on market …

Progress Towards Next Generation Modulators Of Ctbp's Oncogenic Effects, Jacqueline L. West

Theses and Dissertations

Human C-terminal binding proteins (CtBP1 and CtBP2) are transcriptional coregulators of multiple genes in the human genome, including tumor suppressor genes (e.g., Bik, PTEN, BRCA1, and E-cadherin) as well as oncogenes (e.g. MDR1 and Tiam1). Both homologues of CtBP are overexpressed in many types of cancer, including breast cancer (92%), ovarian cancer (83%), colorectal cancer (64%), hepatocellular carcinoma (60%), gastric cancer, prostate cancer, and pancreatic adenocarcinoma. Further, expression levels of CtBP correlate with worse prognostic outcomes and more aggressive tumor features because it promotes proliferation, epithelial-mesenchymal transition, and cancer stem cell self-renewal activity.

Our laboratory has identified a lead inhibitor …

The Influence Of Salinity On The Structure And Function Of Soil Prokaryotic Communities In Coastal Freshwater Wetlands, Joseph C. Morina Ii

Theses and Dissertations

Microbial communities are critical biological components of the world’s ecosystems, and their respiratory pathways are directly involved in the biogeochemical cycling of essential nutrients. As genomic technologies advance, allowing for more detailed profiling of microbial communities, efforts have successfully linked microbial community composition to ecosystem-level functions and have shown microbial communities are susceptible and resistant to disturbance events. The goal of this dissertation is to address the temporal scales in which microbial communities respond to the disturbance of salinization, and the repercussions this has on microbially-meditated carbon and nitrogen cycling. Coastal freshwater wetlands are an excellent study system to investigate …

A Gun For Every Woman, Gentë Retkoceri

Theses and Dissertations

Six centuries ago in Kosova, my home country, unmarried women known as sworn virgins, were forced to adopt a male persona and carry a gun to protect themselves. But today, guns have become tools of oppression and violence against women. And while the legal system carries penalties for domestic violence, marital rape is excluded. To shine a light on, and expose the topic of hidden violence, my thesis uses artivism to address a sensitive but provocative issue, exhibiting a large-scale sculpture of a gun, designed to provoke raw emotions; to challenge visitors to consider the terror felt by women threatened …

Rational Design Of Flexible And Stretchable Electronics Based On 3d Printing, Yuanhang Yang

Theses and Dissertations

Flexible and stretchable electronics have been considered as the key component for the next generation of flexible devices. There are many approaches to prepare the devices, such as dip coating, spin coating, Mayer bar coating, filtration and transfer, and printing, etc. The effectiveness of these methods has been proven, but some drawbacks cannot be ignored, such as lacking pattern control, labor consuming, requiring complex pretreatment, wasting conductive materials, etc.

In this investigation, we propose to adopt 3D printing technology to design flexible and stretchable electronics. The objective is to rationally design flexible and stretchable sensors, simplify the preparation process, form …

Innovative Techniques Of Neuromodulation And Neuromodeling Based On Focal Non-Invasive Transcranial Magnetic Stimulation For Neurological Disorders, Ivan C. Carmona-Tortolero

Theses and Dissertations

This dissertation aims to develop alternative technology that improves the current range of application of transcranial magnetic stimulation (TMS), on a scale that would permit defining specific non-invasive treatments for Parkinson’s disease and other neurological disorders. This is accomplished through three specific objectives. 1) The design of a neurostimulation system that increases the focality in TMS to regions of narrow target areas and variable depths in the brain cortex. 2) The assessment of the feasibility of novel high-frequency neuromodulation techniques that would allow increasing the focality in deeper areas beyond the cortical surface. 3) The development of a computational model …

Improving The Early Detection Of Cardiovascular Toxicity Secondary To Radiotherapy For Lung Cancer Via Patient-Specific Quantitative Magnetic Resonance Imaging, Alireza Omidi

Theses and Dissertations

Purpose: To assess the cardiopulmonary-induced dose variation on the left ventricle (LV) and evaluate the spatiotemporal evolution of cardiac/aortic function following radiotherapy (RT).

Methods: 8 lung cancer patients who were scheduled to receive RT were recruited for this study. Each patient underwent one 4D-CT at baseline. MRI scans including cine GRE, T1/T2, LGE, and 4D-flow were acquired at baseline, 3-months and 6-months post-RT to evaluate the cardiac/aortic function. Finally, image registration was used to assess the cardiopulmonary-induced dose variation on the LV.

Results: Mean RT-dose was minimum during inspiration and systole (at expiration). No significant differences were found in the …

Analysis Of Non-Conventional Radiological Terrorism, James N. Padgett

Theses and Dissertations

Nuclear terrorism has been a risk since the dawn of the first atomic bomb. Though state sponsored nuclear weapons development is of concern for countries, non-state sponsored terrorism with radiological material can be of even greater concern. This stems from the fact that the material is under less stringent or no safeguards and can readily change hands between different terrorist groups or innocent civilians may accidently come into contact with the material.

Within this paper an analysis of previous accidents using orphan radiological sources, malicious use of orphan radiological sources, and how these sources could be used by terrorists is …

Universal Design In Bci: Deep Learning Approaches For Adaptive Speech Brain-Computer Interfaces, Srdjan Lesaja

Theses and Dissertations

In the last two decades, there have been many breakthrough advancements in non-invasive and invasive brain-computer interface (BCI) systems. However, the majority of BCI model designs still follow a paradigm whereby neural signals are preprocessed and task-related features extracted using static, and generally customized, data-independent designs. Such BCI designs commonly optimize narrow task performance over generalizability, adaptability, and robustness, which is not well suited to meeting individual user needs. If one day BCIs are to be capable of decoding our higher-order cognitive commands and conceptual maps, their designs will need to be adaptive architectures that will evolve and grow in …

Characteristics Of Culturally Inclusive Art Education Pedagogy: A Historical Document Analysis Study, Fatemah A. Khawaji

Theses and Dissertations

The objective of this mixed methods historical document analysis is to identify the characteristics of culturally inclusive art education pedagogy. Using the theoretical lenses of intersectionality, critical theory, and socially inclusive pedagogy, this study seeks to determine the reasons for the misapplication of diversity and inclusion in art education. Qualitative and quantitative methods are applied to the historical document analysis of National Art Education Association articles in two major journals: Art Education and Studies in Art Education. The findings indicate that for the last 20 years, 20% of the articles published in Studies in Art Education and 30% of …

Targeting Bcl-2 Family Proteins In Therapy Induced Senescent Cancer Cell Models, Wade Cook

Theses and Dissertations

Non-small Cell Lung Cancer (NSCLC) originates from numerous different cell types in the lungs and is among the deadliest of cancers. Head and Neck Squamous Cell Carcinomas (HNSCC) are derived from the mucosal membranes of the oral cavity, pharynx, and larynx. Both NSCLC and HNSCC are predominately caused by tobacco smoke inhalation and as such mutations in the tumor suppressor gene TP53 are common. Since similarities exist in the root cause of NSCLC and HNSCC, they may also share similarities in treatment methods. Cisplatin is a platinum-based DNA damaging agent that has been used as a cancer chemotherapy for decades. …

Evaluating Population Genetic Structure And Potential Genomic Signals Of Natural Selection In A Migratory Songbird (Protonotaria Citrea), Tyler A. Hohenstein

Theses and Dissertations

In this study I attempted to further resolve the population genetic structure in the Prothonotary Warbler (Protonotaria citrea), and conducted an outlier SNP analysis and exploratory gene ontology analysis to investigate potential ongoing natural selection in the species. This analysis of population structure confirms previous work by DeSaix et al. (2019), where weak population structure was observed between eastern sites along the Atlantic Coastal Plain, and western sites in the Mississippi Alluvial Valley, possibly due to a genetic discontinuity across the Appalachian Mountains. I conducted two forms of outlier SNP analyses, a principal component analysis (PCA)-based approach to identify SNPs …

Development Of Novel, Miniaturized Sperm Cell Isolation Techniques For Sexual Assault Samples, Brittany Celeste Hudson

Theses and Dissertations

The ever-increasing sexual assault evidence collection kit backlog within the United States has prompted the search (and desperate need) for a more efficient, cost-effective, and rapid processing technique that can separate male and female contributions from evidentiary samples. Although backlogs have largely been tackled across the United States and many technological advancements have made the forensic DNA analysis workflow quicker, techniques for handling sexual assault samples remain time-consuming, tedious, and inefficient at separating cellular fractions from the victim and perpetrator. Thus, this research sought to identify a reproducible sperm isolation method using antibodies and/or alternative differential cell lysis techniques as …

Developmental Validation Of A Bacterial Signature-Based Identification Method Of Forensically Relevant Human Biological Samples, Denise M. Wohlfahrt

Theses and Dissertations

The confirmatory identification of body fluids is one of the most critical steps in criminal investigations, as it provides not only information on the source of the DNA, but it can corroborate evidence, aid in the reconstruction of a crime, and associate evidence with a person of interest. Current serological methods utilize biochemistry and immunology to identify biological evidence; however, these serological tests are mostly presumptive while only testing for one fluid at a time, potentially wasting precious evidence. More recently, forensic investigators have suggested the use of molecular methods such as mRNA, miRNA, or DNA methylation. While each of …

Representativeness Of Data From An Online Sample Of Individuals With Severe Alcohol Use Disorder, Michelle Eglovitch

Theses and Dissertations

Understanding the characteristics of research participants who provide DNA is crucial to ensuring sample representativeness and generalizability of findings of genetics studies of substance use disorders. Using anonymous survey data, the present study had a unique opportunity to compare participants who provided DNA (n=2,414) with those that did not provide DNA (n=1,515). The present study found numerous differences between groups across demographic, substance use, and personality domains. Using multivariate regression, the most parsimonious model found that being male, a non-smoker, and endorsing perseverance was associated with providing DNA. The present study provides benchmark data on sample representativeness in a genome …

Development Of A Piezo-Mechanical Battery, Caleb Aba-Onukaogu Aba-Onukaogu

Theses and Dissertations

The piezo-mechanical battery is designed to store energy in a flat spiral spring and harvest the kinetic energy of the unwinding spring using piezoelectric unimorph cantilever beams. The system, initially proposed on a micro scale, was designed to function on a macro scale. The power spring of the system was designed to deliver enough torque to run the system and provide enough force to the escapement mechanism to excite the cantilever beams. The gear system is designed as a compound epicyclic gear system with a gear ratio of 55:1. The cantilever beams were designed to act as springs as well …

The Discovery And Investigation Of Jig (Cg14850): A Previously Uncharacterized Novel Drosophila Melanogaster Protein, Sayem Hoque Bhuiyan

Theses and Dissertations

Despite Drosophila being one of the most studied model organisms in the world of research, one-third of 14,000 protein-encoding genes that the Drosophila genome possesses code for proteins that are yet uncharacterized. While investigating some of these uncharacterized Drosophila proteins, we identified a scarce protein type–a protein localized in both mitochondria and nucleus. Proteins, dual-localized in mitochondria and nucleus, establish communication between these organelles. This type of dual-localized protein is among the rarest of proteins, which limits us from fully understanding the mito-nuclear communication mechanism. Here, we tell the story of Jig, which we discovered to be one of those …

P&I Club Membership As Potential Incentivization For Adherence To Best Space Traffic Management Practices: A Maritime Analogue, Cristin M. Finnigan

Theses and Dissertations

It has been said that the space environment is becoming so accessible, we are at risk of depleting it as a resource, thereby risking society’s space-dependent functions. Law, regulations, policies, and guidelines exist to guide entities to act to preserve the space environment. However, best space traffic management (STM) practice implementation and regulatory compliance could be costly and resource-intensive, especially for a small business. Some entities may not undertake innovative space endeavors at all, or worse, ignore laws, regulations, policies, and guidelines. A question arises of how space actors could be persuaded to work toward meeting STM laws, regulations, policies, …

The Architecture And Dynamics Of Gene Regulatory Networks Directing Cell-Fate Choice During Murine Hematopoiesis, Joanna Handzlik

Theses and Dissertations

Mammals produce hundreds of billions of new blood cells every day througha process known as hematopoiesis. Hematopoiesis starts with stem cells that develop into all the different types of cells found in blood by changing their genome-wide gene expression. The remodeling of genome-wide gene expression can be primarily attributed to a special class of proteins called transcription factors (TFs) that can activate or repress other genes, including genes encoding TFs. TFs and their targets therefore form recurrent networks called gene regulatory networks (GRNs). GRNs are crucial during physiological developmental processes, such as hematopoiesis, while abnormalities in the regulatory interactions of …

An Examination Of The Effects Of Body Mapping Instruction On Singers’ Static Standing Posture And Posture While Singing, Amanda Moreno

Theses and Dissertations

Physical posture is one of the fundamental aspects of vocal technique, and voice pedagogues suggest that effective alignment is necessary for healthy vocal production. Body Mapping (BMG) is a somatic method that focuses on the understanding and correction of errors in a person’s body map to facilitate effective movement for musical activity. Because this method has balance, physical alignment, and posture as foundational, it may be an effective way to begin to instruct students. This study investigated whether the use of the BMG method, which teaches posture through movement, scientific pictures, and anatomical models, would result in a significant difference …

Mosquito Fauna And Prevalence Of Avian Malaria In The Turtle Mountains, North Dakota, Kelsey J. Morin

Theses and Dissertations

Avian malaria is a disease caused by intracellular parasites transmitted by mosquitoes and is detrimental to individual birds and their populations. Although it is sub-lethal in most instances, avian malaria can result in anemia, lower fertility, and reduced migration ability. The Turtle Mountains is a unique ecoregion in North Dakota and provides habitat for various bird and mosquito species that differ from other parts of the state. Previous research has defined the mosquito fauna and occurrence of avian malaria in the Red River Valley region of eastern North Dakota and Northwest Minnesota; however, no research has been conducted in the …

Behavioral Responses Of Breeding Ducks To Unmanned Aerial Vehicle Surveys And Best Practices For Breeding Waterfowl Surveys Using Unmanned Aerial Vehicles, Mason Ryckman

Theses and Dissertations

Unmanned aerial vehicles (UAVs) have become a popular wildlife survey tool. As such, biologists are exploring the use of UAVs for surveying waterfowl. The most cited benefit of using UAVs over traditional methods is the idea of reduced disturbance, but this has had limited formal evaluation across species. We conducted UAV surveys with associated behavioral observations of ducks on wetlands and on nests during the 2019 – 2020 breeding seasons. We found species-specific behaviors among blue-winged teal (Spatula discors), northern shoveler (Spatula clypeata), and gadwall (Mareca strepera) including ducks noticing the aircraft, but reactions were generally less than traditional ground …

Phylogenetic Relationships Of Noctuoid Moths With An Examination Of Complex Characteristics, Melissa Sisson

Theses and Dissertations

The tribe Noctuini comprise over 520 species; many are economically important species that impact human agriculture. Despite their diversity and relevance, relationships of the Noctuini have been difficult to resolve. There are extensive morphological revisions of these taxa (e.g., Agrotis, Euxoa); however, there are no studies that focus exclusively on the phylogenetic relationships of the Noctuini. Currently, the Noctuini are separated into two subtribes, the Agrotina and Noctuina, which each are further subdivided into two subgenera. In this study, we tested previous classification schemes using three loci. We sampled three outgroup taxa and 54 noctuine species that represent the majority …

A Survey Of Research Administrators To Identify Areas Of Administrative Burden In Federal Research Grant Management, Jessica Lynn Schiller

Theses and Dissertations

Many processes in higher education are complicated by administrative burden and proceduralinefficiencies. The federal government and research universities partner to advance science and technology, but the federal research grant process is criticized for its lack of efficient and streamlined procedures. Staff and faculty alike lament losing valuable time to bureaucratic regulations, cumbersome processes, and burgeoning reporting standards. As the cost of higher education is increasingly scrutinized, current procedures must be analyzed to identify areas of excessive administrative burden and redesigned to maximize efficiency. To address this issue, a Qualtrics survey was deployed to research administrators to identify areas of administrative …

A Technology-Based, Mixed Methods Approach To Examining The Psychosocial Determinants Of Maternal Health Disparities, Hannah M. Ming

Theses and Dissertations

Background: Exposure to racism and discrimination in the U.S. increases Black women’s risk for experiencing maternal health disparities. Additionally, racism and discrimination affect maternal psychosocial well-being, creating evidence for a biopsychosocial relationship between racism and maternal health outcomes. However, current research does not define the psychosocial Black maternal self well. Given the dynamic relationship between racism, psychosocial well-being, and Black maternal health outcomes, research must comprehensively examine the Black maternal self. The operationalization of a comprehensive construct for Black maternal psychosocial well-being can improve understanding of the relationship between racism, psychosocial well-being, and Black maternal health outcomes.

Purpose: …