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In-Plane, All-Photonic Transduction Method For Silicon Photonic Microcantilever Array Sensors, Jong Wook Noh Nov 2009

In-Plane, All-Photonic Transduction Method For Silicon Photonic Microcantilever Array Sensors, Jong Wook Noh

Theses and Dissertations

We have invented an in-plane all-photonic transduction method for photonic microcantilever arrays that is scalable to large arrays for sensing applications in both bio- and nanotechnology. Our photonic transduction method utilizes a microcantilever forming a single mode rib waveguide and a differential splitter consisting of an asymmetric multimode waveguide and a Y-branch waveguide splitter. The differential splitter's outputs are used to form a differential signal that has a monotonic response to microcantilever deflection. A differential splitter using an amorphous silicon strip-loaded multimode rib waveguide is designed and fabricated to demonstrate the feasibility of the in-plane photonic transduction method. Our initial …

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Preparation And Characterization Of Stable Macroporous Titania Nano-Wells, Hema Santhi Aluri Nov 2009

Preparation And Characterization Of Stable Macroporous Titania Nano-Wells, Hema Santhi Aluri

Theses and Dissertations

Silica based sol-gel thin films have been extensively studied because of their advantages that include optical transparency, high biodegradability and low intrinsic fluorescence. However, one concern with silica based sol-gel derived materials is their long term stability in aqueous solutions. Another concern is their limited porosity. These two concerns limit the application of these materials in catalysis and separations. The main objective of this study is to prepare porous, thin films using titanium alkoxides as precursors and evaluate their long term stability in aqueous solutions. Colloidal crystal templating will be used to introduce macrosized pores into the titania network. The …

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Assessment Of Perceived Positive And Negative Outcomes In Risky Adolescent Dating And Peer Situations: A Descriptive Analysis Of Risk And Benefit Perception, Sarah Helms Nov 2009

Assessment Of Perceived Positive And Negative Outcomes In Risky Adolescent Dating And Peer Situations: A Descriptive Analysis Of Risk And Benefit Perception, Sarah Helms

Theses and Dissertations

Peer and dating violence perpetration and victimization are common experiences placing adolescents at-risk for maladaptive outcomes, yet little research has focused on specific problematic situations associated with these types of violence and other related risk-taking behaviors. Further, prevention programs have typically focused on changing beliefs, attitudes, and skill-deficits, with little attention to adolescents’ motivations or perceptions of costs and benefits associated with risky situations, despite support for this type of research in other health-related fields (e.g., substance abuse, behavioral medicine). The current study assessed adolescents’ perceptions of potential positive and negative outcomes associated with risky dating and peer situations, many …

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You Don't Know Jack: The Dynamics Of Mormon Religious/Ethnic Identity, Michael R. Cope Nov 2009

You Don't Know Jack: The Dynamics Of Mormon Religious/Ethnic Identity, Michael R. Cope

Theses and Dissertations

For much of human existence identity was ascribed based on the group one was born into. In such cultures all aspects of social life were fused into one incontrovertible identity: group identity. However as modern mindsets took root individuals began to shift the foundation of meaning and identity away from the fixed focal point of the group to one of personal preference. In response to this modern trend many groups began to intensify the maintenance of group identity as paramount in the lives of group members. Hammond and Warner (1993) assert that a powerful mechanism for sustaining group identity is …

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Analysis Of The Regulation Of The Transferrin Iron Acquisition System In Neisseria Gonorrhoeae, Acevedo Rosuany Vélez Nov 2009

Analysis Of The Regulation Of The Transferrin Iron Acquisition System In Neisseria Gonorrhoeae, Acevedo Rosuany Vélez

Theses and Dissertations

The neisserial transferrin binding proteins (Tbps) comprise a bipartite system for iron acquisition from human transferrin. TbpA is a TonB-dependent outer membrane protein that forms the pore for iron internalization. TbpB is a surface-exposed lipoprotein that makes the iron-uptake process more efficient. Previous studies have shown that the genes encoding these proteins are arranged in a bicistronic operon, with the tbpB gene located upstream of tbpA. The operon is under the control of the ferric uptake regulator (Fur) protein. However, promoter elements necessary for the regulation of the operon have not been experimentally defined. In this study, putative regulatory motifs …

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Ethnic Effects On Biological, Psychosocial And Disease Care Factors In African-American Youth With Type 1 Diabetes, Priscilla Powell Nov 2009

Ethnic Effects On Biological, Psychosocial And Disease Care Factors In African-American Youth With Type 1 Diabetes, Priscilla Powell

Theses and Dissertations

Person-oriented and variable-oriented analyses were conducted to investigate sociodemographic differences in biological, psychosocial and disease care factors in youth with type 1 diabetes. Additionally, diabetes knowledge was evaluated as a potential mediator of SES effects on HbA1c and disease care. The sample included 349 youth, age 9-17 years (79.9% Caucasian, 71.3% lived with two biological parents, M SES = 46.24). Person-oriented t-tests confirmed commonly reported ethnic differences in HbA1c and disease care behaviors. However, variable-oriented analyses controlling for confounding sociodemographic influences showed most disease care effects attributed to ethnicity were better explained by SES. While diabetes knowledge was not a …

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Defining The Role Of Citizens In Dialogic Environmental Risk Communication, Holly Lynn Anderson Nov 2009

Defining The Role Of Citizens In Dialogic Environmental Risk Communication, Holly Lynn Anderson

Theses and Dissertations

Risk communication has evolved into an interdisciplinary study involving the work of communication specialists, psychologists, attorneys, and rhetoricians. Of particular interest has been the interaction between government and citizens in environmental risk communication. This interaction has been characterized by citizen outrage in response to dictatorial governmental decision-making. In response, researchers have called for true two-way dialogue between government and the public. This project addressses risk communication during the permitting of hazardous waste management facilities under the Resource Conservation and Recovery Act, identifying the failure to implement two-way dialogue. The project addresses two concerns: (1) the impact of existing legal barriers, …

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Synthesis And Evaluation Of N6,5'-Bis-Ureido-5'-Amino-5'-Deoxyadenosine Derivatives: Novel Nucleosides With Antiproliferative And Protein Kinase Binding Activities, Marcelio Oliveira Nov 2009

Synthesis And Evaluation Of N6,5'-Bis-Ureido-5'-Amino-5'-Deoxyadenosine Derivatives: Novel Nucleosides With Antiproliferative And Protein Kinase Binding Activities, Marcelio Oliveira

Theses and Dissertations

A new series of N6,5'-bis-ureido-5'-amino-5'-deoxyadenosine derivatives was prepared and evaluated for anticancer activities using the NCI 60 panel of human cancers. Certain of the derivatives showed promising activities (low micromolar GI50's) against several of the representative cancers. These included cell lines from the following general cell types in the NCI 60: Leukemia, Breast, Central Nervous System, Non-Small Cell Lung, Ovarian, Prostate, Renal, and Colon cancers. Select compounds were also screened for their affinities for protein kinases. The synthesis of the compounds was straightforward and involved N6 acylation with arylisocyanates, preceded by activation and nucleophilic substitution of the 5'-position to give …

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Bpv Entry And Trafficking In Ebtr Cells, Enkhmart Dudleenamjil Nov 2009

Bpv Entry And Trafficking In Ebtr Cells, Enkhmart Dudleenamjil

Theses and Dissertations

Bovine Parvovirus (BPV) belongs to the genus Bocavirus, family Parvoviridae. BPV is the leading etiologic agent among the pathogens that cause primary gastroenteritis of cattle. Many of the intracellular events associated with virus replication are unknown. In this research project, we investigated BPV internalization into the host cell and trafficking in the cytosol. Preliminarily, EBTr cells had abundant clathrin, virus attached to purified clathrin, and EM micrographs revealed virus in endocytic vacuoles. Assays detecting virus infectivity (i.e. viral protein synthesis), virus production (completion of the replication cycle), and quantitative PCR (qPCR) to detect viral transcripts were used to evaluate …

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Optimization Of Functional Mri Methods For Olfactory Interventional Studies At 3t, Vishwadeep Ahluwalia Nov 2009

Optimization Of Functional Mri Methods For Olfactory Interventional Studies At 3t, Vishwadeep Ahluwalia

Theses and Dissertations

Functional MRI technique is vital in investigating the effect of an intervention on cortical activation in normal and patient population. In many such investigations, block stimulation paradigms are still the preferred method of inducing brain activation during functional imaging sessions because of the high BOLD response, ease in implementation and subject compliance especially in patient population. However, effect of an intervention can be validly interpreted only after reproducibility of a detectable BOLD response evoked by the stimulation paradigm is first verified in the absence of the intervention. Detecting a large BOLD response that is also reproducible is a difficult task …

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Predictive Modeling Of Sulfur Flower Buckwheat (Erigonum Umbellatum Torrey) Using Non-Parametric Multiplicative Regression Analysis, David B. Davis Nov 2009

Predictive Modeling Of Sulfur Flower Buckwheat (Erigonum Umbellatum Torrey) Using Non-Parametric Multiplicative Regression Analysis, David B. Davis

Theses and Dissertations

Impacts of humans on ecosystems in western United States have necessitated ecological restoration, which includes the development of native seed that can be used for revegetation efforts. Development of such seed sources are costly and time consuming. This study describes the use of non-parametric multiplicative regression analysis (NPMR) to develop a predictive model for occurrence of sulfur-flower buckwheat (Eriogonum umbellatum Torrey) population seed collection. This perennial forb species is of interest for seed source development in the western United States. Presence and absence data for E. umbellatum was taken from the Utah Division of Wildlife Resources Big Game Range …

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Emerging Adulthood In India, Liann Nicole Seiter Nov 2009

Emerging Adulthood In India, Liann Nicole Seiter

Theses and Dissertations

This paper explores the nature of emerging adulthood in Southern India. Survey data was collected from 450 college students in Coimbatore, Tamil Nadu, India and 100 non-students from rural villages surrounding Coimbatore. Unlike American samples, the majority of the 18- to 26-year-olds studied felt that they had achieved adulthood. The sample emphasized attributes needed to fulfill family roles as characteristics necessary for adulthood. Differences in optimism levels were found between students and non-students. Arnett suggests that emerging adulthood would be affected by cultural influences. The unique cultural and structural influences in India such as, Hinduism, caste, gendered socialization, and the …

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Remembering The Ghost: Pedro Páramo And The Ethics Of Haunting, Benjamin Cluff Nov 2009

Remembering The Ghost: Pedro Páramo And The Ethics Of Haunting, Benjamin Cluff

Theses and Dissertations

This study seeks to describe what I term the ethics of haunting, as related to trauma and memory, by analyzing Juan Rulfo's Pedro Páramo. It does not claim to be representative of ghosts and haunting as a whole, but more specifically to illustrate various manners in which the return of the ghost and its subsequent haunting are motivated by an ethics of memory in Rulfo's novel. Within this framework I explore remembrance as a medium of exchange between the living and the dead, haunting as a method by which gaps in the historical archive can be filled, and the …

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Quantitative Analysis Of Tobacco Specific Nitrosamine In Human Urine Using Molecularly Imprinted Polymers As A Potential Tool For Cancer Risk Assessment, Kumar Shah Nov 2009

Quantitative Analysis Of Tobacco Specific Nitrosamine In Human Urine Using Molecularly Imprinted Polymers As A Potential Tool For Cancer Risk Assessment, Kumar Shah

Theses and Dissertations

Measuring urinary tobacco specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its glucuronide conjugate may provide the best biomarker of tobacco smoke lung carcinogen metabolism. Existence of differences in the extent of NNAL metabolism rates may be potentially related to an individuals’ lung cancer susceptibility. Low concentrations of NNAL in smokers urine (<1 ng/mL) require sensitive and selective methods for analysis. Traditionally, this involves extensive, time-consuming sample preparation that limits throughput and adds to measurement variability. Molecularly imprinted polymers (MIPs) have been developed for the analysis of urinary NNAL by offline cartridge extraction combined with LC-MS/MS. This method when reproduced demonstrated problems with matrix effects. In the first part of this work, investigation of matrix effects and related problems with sensitivity for the published offline extraction method has been conducted. In order to address the need to improve throughput and other analytical figures of merit for the original method, the second part of this work deals with development of a high-throughput online microfluidic method using capillary-columns packed with MIP beads for the analysis of urinary NNAL. The method was validated as per the FDA guidance, and enabled low volume, rapid analysis of urinary NNAL by direct injection on a microfluidic column packed with NNAL specific MIP beads. The method was used for analysis of urinary NNAL and NNAL-Gluc in smokers. Chemometric methods were used with this data to develop a potential cancer-risk-assessment tool based on pattern recognition in the concentrations of these compounds in urine. In the last part, method comparison approaches for the online and the offline sample extraction techniques were investigated. A ‘fixed’ range acceptance criterion based on combined considerations of method precision and accuracy, and the FDA bioanalytical guidance limits on precision and accuracy was proposed. Data simulations studies to evaluate the probabilities of successful transfers using the proposed criteria were performed. Various experimental designs were evaluated and a design comprised of 3 runs with 3 replicates each with an acceptance range of ±20% was found appropriate. The off-line and the on-line sample extraction methods for NNAL analysis were found comparable using the proposed fixed range acceptance criteria.

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Robust Protective Factors That Help Youths With A Parent Experiencing Depression Achieve Positive Adjustment, Hsing-Jung Chen Nov 2009

Robust Protective Factors That Help Youths With A Parent Experiencing Depression Achieve Positive Adjustment, Hsing-Jung Chen

Theses and Dissertations

Based on a resilience framework, the purpose of this study was to address knowledge gaps about minority youths who lived in rural and poor areas, had a primary caregiver with a diagnosis of depression, and faced multiple psychosocial stressors. Three research objectives included: 1) To explore the association between ecological protective factors and four developmental outcomes-emotional adjustment, behavioral adjustment, school performance, and educational aspiration; 2) To identify the robust protective factors; and 3) To explore the interactive relationships between risk and robust protective factors. Families (N=126) where the primary caregiver had a diagnosis of major depression and had a child …

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Nonlinear Models In Multivariate Population Bioequivalence Testing, Bassam Dahman Nov 2009

Nonlinear Models In Multivariate Population Bioequivalence Testing, Bassam Dahman

Theses and Dissertations

In this dissertation a methodology is proposed for simultaneously evaluating the population bioequivalence (PBE) of a generic drug to a pre-licensed drug, or the bioequivalence of two formulations of a drug using multiple correlated pharmacokinetic metrics. The univariate criterion that is accepted by the food and drug administration (FDA) for testing population bioequivalence is generalized. Very few approaches for testing multivariate extensions of PBE have appeared in the literature. One method uses the trace of the covariance matrix as a measure of total variability, and another uses a pooled variance instead of the reference variance. The former ignores the correlation …

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Speaking Out: How Women Create Meaning From The Dove Campaign For Real Beauty, Valori Infanger Nov 2009

Speaking Out: How Women Create Meaning From The Dove Campaign For Real Beauty, Valori Infanger

Theses and Dissertations

The purpose of this study was to determine whether the Dove Campaign for Real Beauty could be used to effectively expand the media-narrowed definition of beauty. This study focused on the Onslaught video and corresponding message board. The sample included 119 posts written by 85 different message board users. Both a descriptive and interpretive content analysis based on reception analysis was used to deconstruct the message posters' interpretation and construction of individual beauty. The posters used the board primarily to express themselves, attach blame to the media and arrive at consensus. Overall, the users responded positively to the campaign. Twenty …

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The Genetic Epidemiology Of Purging Disorder, Anorexia Nervosa, And Obsessive Compulsive Personality Disorder, Sara Trace Nov 2009

The Genetic Epidemiology Of Purging Disorder, Anorexia Nervosa, And Obsessive Compulsive Personality Disorder, Sara Trace

Theses and Dissertations

Although a variety of factors influence the development of eating disorders, genetic factors contribute notably to their etiology. Understanding genetic factors associated with eating disorders is important, as they can influence how these disorders are recognized, researched, and treated. This dissertation included two studies addressing important questions within the fields of eating disorders and genetics; specifically, Study 1 addressed the prevalence and heritability of purging and purging disorder in a population-based sample of female twins from the United States; and Study 2 investigated the nature of the co-morbidity between anorexia nervosa and obsessive compulsive personality disorder in a population-based sample …

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Dilute Sulfuric Acid Pretreatment Of Switchgrass In Microwave Reactor For Biofuel Conversion: An Investigation Of Yields, Kinetics,, Oscar Martin Nov 2009

Dilute Sulfuric Acid Pretreatment Of Switchgrass In Microwave Reactor For Biofuel Conversion: An Investigation Of Yields, Kinetics,, Oscar Martin

Theses and Dissertations

Lignocellulosic materials provide a raw material source for biofuel conversion and offer several advantages over fossil fuels- usage of a renewable resource, reduced greenhouse emissions, a decreased dependence on foreign oil, and stimulation of the agricultural sector. However, a primary technological challenge in converting lignocellulosic biomass into fuel is overcoming the recalcitrance of its matrix to enzymatic hydrolysis. To overcome these problems for chemical processing, naturally occurring cellulose biomass must be pretreated before it can be further processed using enzymatic hydrolysis or bioconversion. The goal of this work was to develop a model that predicts the glucose yield (pretreatment and …

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Nonhomologous End-Joining: Tdp1-Mediated Processing, Atm-Mediated Signaling, Amy Hawkins Nov 2009

Nonhomologous End-Joining: Tdp1-Mediated Processing, Atm-Mediated Signaling, Amy Hawkins

Theses and Dissertations

This thesis investigates two separate features of nonhomologous end-joining (NHEJ) DNA repair: end processing, and DNA repair kinase signaling. DNA end processing was investigated in a mouse model of hereditary spinocerebellar ataxia with axonal neuropathy (SCAN1), a congenital neurodegenerative disease. SCAN1 is caused by a homozygous H493R mutation in the active site of tyrosyl-DNA phosphodiesterase (TDP1). To address how the H493R mutation elicits the specific pathologies of SCAN1 and to further elucidate the role of TDP1 in processing DNA end modifications, we generated a Tdp1 knockout mouse and characterized their behavior and specific repair deficiencies in extracts of embryonic fibroblasts …

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Part 1: Screening Of Thirty-One Medicinal Plant Species Against Herpes Simplex Virus, Acetone And Methanol Extracts From The Root Tissue Of Kalanchoe Pinnata Interferes With Hsv Types 1 And 2 Dna Replication And Early And Late Gene Expression Preventing The Spread Of Hsv In Vitro. Part 2: Professional Development Curriculum: Integrating Molecular Biology And Microbiology Into The Existing Secondary Biology Curricula, Mary Ruth Greer Nov 2009

Part 1: Screening Of Thirty-One Medicinal Plant Species Against Herpes Simplex Virus, Acetone And Methanol Extracts From The Root Tissue Of Kalanchoe Pinnata Interferes With Hsv Types 1 And 2 Dna Replication And Early And Late Gene Expression Preventing The Spread Of Hsv In Vitro. Part 2: Professional Development Curriculum: Integrating Molecular Biology And Microbiology Into The Existing Secondary Biology Curricula, Mary Ruth Greer

Theses and Dissertations

PART 1: Thirty-one medicinal plant species from Hawaii, Morocco, and the Sonoran Desert, USA have been shown in past studies to be highly inhibitory to pathogenic bacteria, fungi, and certain cancer cell lines. However, none were tested for antiviral activity. Acetone and methanol extracts from these species were bio-assayed for antiviral activity against herpes simplex virus types 1 and 2 (HSV 1 and HSV 2) and for cytotoxicity to the Vero C1008 cell line. Extracts from these species were tested in vitro for antiviral activity using an immunoperoxidase mini-plaque reduction assay to detect viral structural protein synthesis. Sulforhodamine B and …

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Joint Mixed-Effects Models For Longitudinal Data Analysis: An Application For The Metabolic Syndrome, John Thorp Iii Nov 2009

Joint Mixed-Effects Models For Longitudinal Data Analysis: An Application For The Metabolic Syndrome, John Thorp Iii

Theses and Dissertations

Mixed-effects models are commonly used to model longitudinal data as they can appropriately account for within and between subject sources of variability. Univariate mixed effect modeling strategies are well developed for a single outcome (response) variable that may be continuous (e.g. Gaussian) or categorical (e.g. binary, Poisson) in nature. Only recently have extensions been discussed for jointly modeling multiple outcome variables measures longitudinally. Many diseases processes are a function of several factors that are correlated. For example, the metabolic syndrome, a constellation of cardiovascular risk factors associated with an increased risk of cardiovascular disease and type 2 diabetes, is often …

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Intraspecific Phylogeography Of Cycladenia Humilis (Apocynaceae), Mariana P. Last Nov 2009

Intraspecific Phylogeography Of Cycladenia Humilis (Apocynaceae), Mariana P. Last

Theses and Dissertations

Cycladenia humilis (Apocynaceae) is a rare perennial herb native to western North America and has a fragmented distribution in California, Utah, and Arizona. Populations in Utah and Arizona are federally listed as threatened, while there is no conservation status applied to California populations. Using genetic (three chloroplast and two nuclear DNA loci) and morphological characters, intraspecific variation between populations of C. humilis and current taxonomic conventions were assessed. Nested Clade Phylogeographic Analysis and Bayesian phylogenies were used to assess patterns within C. humilis and supported three main population groupings: a northern California, southern California, and Colorado Plateau group. The northern …

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Optimizing The Release And Methylation Of Bacterial Endospore Dipicolinic Acid, Aaron N. Nackos Nov 2009

Optimizing The Release And Methylation Of Bacterial Endospore Dipicolinic Acid, Aaron N. Nackos

Theses and Dissertations

Rapid, portable detection of biological threat agents such as Bacillus anthracis endospores (“spores”) is extremely important given the real and perceived threats of bioterrorism. Gas chromatography-mass spectrometry (GC-MS) is an excellent general means for chemical detection, although special sample preparation and specialized equipment are required to employ GC-MS for detecting biological agents such as spores in the field. A GC sample introduction probe consisting of a helical wire that can be retracted inside a syringe needle, called a coiled wire filament (CWF), was employed as a simple, passively-heated means to introduce the mixture of spores plus reagents for thermochemolysis methylation …

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Mechanical Behavior And Length Adaptation Of Rabbit Bladder Smooth Muscle, Atheer Almasri Oct 2009

Mechanical Behavior And Length Adaptation Of Rabbit Bladder Smooth Muscle, Atheer Almasri

Theses and Dissertations

Overactive bladder (OAB), involuntary contractions during bladder filling, is a common condition affecting 17% of the adult population worldwide, and in the U.S. ranks ahead of diabetes in a list of the 10 most common chronic disorders (Mullins 2009). Mechanical mechanisms contributing to OAB are not completely understood and because of the unique function and broad volume range of the bladder, there may be mechanical characteristics that distinguish detrusor smooth muscle (DSM) in bladder from other smooth muscles. Recent studies have shown that the length-passive tension curve in DSM exhibits adjustable passive stiffness (APS) characterized by a passive curve that …

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Systematics And Biogeography Of The Didymopanax Group Of Schefflera (Araliaceae), Pedro Fiaschi Oct 2009

Systematics And Biogeography Of The Didymopanax Group Of Schefflera (Araliaceae), Pedro Fiaschi

Theses and Dissertations

Schefflera is the largest genus in the angiosperm family Araliaceae, with about 900 species, of which c. 300 belong to five subgeneric groups in the Neotropical region. Previous phylogenetic studies of Schefflera have been limited to a small number of species from this region, and very little is know about phylogenetic relationships in the Brazilian-centered Didymopanax group of this genus. Therefore, to gain a better understanding of the diversity and evolution of the Didymopanax group of Schefflera, I investigated the systematics of these plants in the broader context of the entire Neotropical clade. The main goals were (1) to investigate …

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Isotopic Evaluation Of Carbon Dioxide In Soil Gas In Utah For A More Accurate Input Variable In Groundwater Age Determining Models, Rachelle Hart Oct 2009

Isotopic Evaluation Of Carbon Dioxide In Soil Gas In Utah For A More Accurate Input Variable In Groundwater Age Determining Models, Rachelle Hart

Theses and Dissertations

In order to achieve a more accurate input value for groundwater age determining models, δ13CVPDB values for soil gas were evaluated at 50 cm depths in locations throughout Utah in order to define correlations between δ13C and environmental parameters. 16 sites were chosen that exploited large changes in elevation and latitude which provided variations in climate, precipitation, plant community, etc. Gas samples were collected over 1-1½ years, and soil samples were collected at depth during installations. Field and laboratory studies were also used to evaluate CO2 and δ13C change with depth. It was discovered that in mountainous recharge areas, the …

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Sphingolipid-Induced Activation Of The Volume-Sensitive Cl− Current Is Mediated By Mitochondrial Reactive Oxygen Species, Frank Raucci Oct 2009

Sphingolipid-Induced Activation Of The Volume-Sensitive Cl− Current Is Mediated By Mitochondrial Reactive Oxygen Species, Frank Raucci

Theses and Dissertations

Swelling-activated Cl− current (ICl,swell) is an outwardly-rectifying current that plays an important role in cardiac electrical activity, cellular volume regulation, apoptosis, and acts as a potential effector of mechanoelectrical feedback. Persistent activation of ICl,swell has been observed in a number of models of cardiovascular disease. Previously we showed that angiotensin II (Ang II), endothelin-1 (ET-1), endothelial growth factor receptor (EGFR), and reactive oxygen species (ROS) produced by NADPH oxidase (NOX) and mitochondria are involved in the activation of ICl,swell by both osmotic swelling and Beta1 integrin stretch. Sphingolipid metabolism is modulated in several cardiopathologies and because sphingolipids are bioactive lipids …

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Pore Size Characterization Of Monolithic Capillary Columns Using Capillary Flow Porometry, Yan Fang Sep 2009

Pore Size Characterization Of Monolithic Capillary Columns Using Capillary Flow Porometry, Yan Fang

Theses and Dissertations

A simple capillary flow porometer (CFP) was assembled for pore structure characterization of monolithic capillary liquid chromatography columns based on ASTM standard F316-86. Determination of differential pressures and flow rates through dry and wet samples provided the necessary information to determine the through-pore throat diameter, bubble point pore diameter, mean flow pore diameter, and pore distribution. Unlike measurements in bulk using traditional techniques to provide indirect information about the pore properties of monolithic columns, monoliths can be characterized in their original chromatographic forms with this system. The performance of the new CFP was first evaluated by characterizing the pore size …

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The Regulation Of Alternative Splicing By Oncogenic Signaling Pathways., Jacqueline Shultz Sep 2009

The Regulation Of Alternative Splicing By Oncogenic Signaling Pathways., Jacqueline Shultz

Theses and Dissertations

In the presented study, we demonstrate that the alternative splicing of caspase 9 was dysregulated in a large percentage of NSCLC tumors and cell lines. These findings led to the hypothesis that survival pathways activated by oncogenic mutation regulated this mechanism. Indeed, the oncogenic PI3-Kinase/Akt pathway was demonstrated to regulate the alternative splicing of caspase 9. Further mechanistic studies demonstrate that multiple Akt isoforms can regulate the alternative splicing of caspase 9 in NSCLC. Akt was additionally shown to mediate the exclusion of the exon 3,4,5,6 cassette of caspase 9 via the phospho-state of the RNA trans-factor, SRp30a. Mutagenesis studies …

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