Digital Commons Network^™

Identifying With The Help: An Examination Of Class, Ethnicity And Gender On A Post-Colonial French Houselot, Erin Nicole Whitson

Theses and Dissertations

The purpose of this study is to attempt to analyze and interpret an outbuilding located in Ste. Genevieve, Missouri, a town established by French settlers in the 1780s. First the functional activities associated with the outbuilding will be determined and examined to best understand where and how the structure fit within the larger property. The activities taking place within/outside the outbuilding--especially those linked to domestic chores--will theoretically paint a clearer picture of who was living and/or working within the structure and who was not. Since little work has been done within the French Mississippi River valley on outbuildings in general, …

Dynamic Nanochannels For Biosensing Applications, Joseph B. Oxborrow

Theses and Dissertations

Inexpensive label-free detection of biomarker panels in serum could revolutionize earlycancer diagnosis and treatment. Such detection capabilities may be possible with dynamicnanochannels in conjunction with electrical impedance measurement. In Dr. Greg Nordin's lab I designed, fabricated and tested several iterations of these sensors with polydimethyl-siloxane microfluidics. The final design yielded a dynamic nanochannel array sensor thatshowed a 140% impedance change when exposed to 14µM bovine serum albumin in phos-phate buffered saline. For the geometry and noise limits of the tested device, simulationsindicated that a minimum detectable concentration of 20pM with specifically bound strep-tavidin should be possible. However, the polydimethylsiloxane approach …

Analyzing The Functions Of Human Polynucleotide Phosphorylase (Hpnpaseold-35), Upneet K. Sokhi

Theses and Dissertations

RNA degradation plays a fundamental role in maintaining cellular homeostasis, along with being a part of normal regulatory mechanisms, whether it occurs as a surveillance mechanism eliminating aberrant mRNAs or during RNA processing to generate mature transcripts. 3’-5’ exoribonucleases are essential mediators of RNA decay pathways, and one such evolutionarily conserved enzyme is polynucleotide phosphorylase (PNPase). The human homologue of this fascinating enzymatic protein (hPNPaseold-35) was cloned a decade ago in the context of terminal differentiation and senescence through a novel ‘overlapping pathway screening’ approach. Since then, significant insights have been garnered about this exoribonuclease and its repertoire of expanding …

The Association Between Dairy Consumption And Insulin Resistance, Andrea Rose Erickson

Theses and Dissertations

Background: A cross-sectional design was employed to ascertain the relationship between dairy consumption and insulin resistance (IR) in 272 middle-aged, nondiabetic women. Methods: Participants kept a seven-day weighed food record to report their diets, including consumption of dairy foods. IR was assessed using the homeostatic model assessment (HOMA), using the following formula: fasting plasma insulin (µU/ml) x fasting plasma glucose (mg/dL)/405. The Bod Pod was used to examine body fat percentage, and accelerometry over a seven-day period was used to assess physical activity. HOMA values were log-transformed and regression analysis and the General Linear Model procedure were used to determine …

Laser Vaporization Methods For The Synthesis Of Metal And Semiconductor Nanoparticles; Graphene, Doped Graphene And Nanoparticles Supported On Graphene, Parichehr Afshani

Theses and Dissertations

The major objective of the research described in this dissertation is the development of new laser vaporization methods for the synthesis of metal and semiconductor nanoparticles, graphene, B- and N-doped graphene, and metal and semiconductor nanoparticles supported on graphene. These methods include the Laser Vaporization Controlled Condensation (LVCC) approach, which has been used in this work for the synthesis of: (1) gold nanoparticles supported on ceria and zirconia nanoparticles for the low temperature oxidation of carbon monoxide, and (2) graphene, boron- and nitrogen-doped graphene, hydrogen-terminated graphene (HTG), metal nanoparticles supported on graphene, and graphene quantum dots. The gold nanoparticles supported …

Α-Hydroxyhippuric Acid Derivatives: Ph-Dependent Aqueous Kinetics And Buffer Catalysis., Kati Feken

Theses and Dissertations

Synthesis and kinetic investigations of the aqueous breakdown of α-hydroxyhippuric acid derivatives as a function of pH were performed to acquire an understanding of the reactivity of this biologically relevant carbinolamide functionality.

A Molecular Mechanism Regulating The Timing Of Corticogeniculate Innervation, Justin Brooks

Theses and Dissertations

Visual system development requires the formation of precise circuitry in the dorsal lateral geniculate nucleus (dLGN) of the thalamus. Although much work has examined the molecular mechanisms by which retinal axons target and form synapses in dLGN, much less is known about the mechanisms that coordinate the formation of non-retinal inputs in dLGN. These non-retinal inputs represent ~90% of the terminals that form in dLGN. Interestingly, recently reports show that the targeting and formation of retinal and non-retinal inputs are temporally orchestrated. dLGN relay neurons are first innervated by retinal axons, and it is only after retinogeniculate synapses form that …

The Development Of A Discipline: Examination Of The Profession Of Gerontology And Gerontological Professionals, Tracey Gendron

Theses and Dissertations

The growth of the aging population has warranted increased training and education to prepare professionals with the specific knowledge needed to best serve older adults. Gerontology, as an academic discipline, provides professionals with the conceptual knowledge and the skills necessary to address the complexities of working with a diverse aging population. Little research has been done of the characteristics of professionals both with and without formal education in gerontology that are working with the aging population. The aim of this study was to investigate the roles of career motivation, job satisfaction, attitudes about aging, career commitment, and professional identity among …

An Investigation Into The Presynaptic Actions By Which Amphetamine Activates Dopamine Signaling Within Striatal Subregions Of The Rat., Dan P. Covey

Theses and Dissertations

Despite decades of intense research and a consensus view in the field, the work presented herein provides strong evidence that the primary mechanism of amphetamine action should be re-evaluated. Rather than depleting vesicular dopamine stores and promoting non-exocytotic efflux through the dopamine transporter in vivo, recent evidence demonstrates that amphetamine augments phasic dopamine signaling instead. This signaling modality is critical for reinforcement learning and is dependent on intact vesicular stores. The new findings support over-activation of phasic dopamine signaling as a common mechanism in the addiction process.

Effects Of Nitrite And Oxygen On Angiogenesis In Vascular Networks Of The Chicken Embryo, Michael Connery

Theses and Dissertations

Nitric oxide (NO) is an important mediator of angiogenesis and is primarily produced endogenously through the action of nitric oxide synthase (NOS). An alternate pathway for NO production is the conversion of nitrite to NO, which depends on the presence of hemoglobin (Hb) and hypoxic conditions. The angiogenic effects of topically applied sodium nitrite on two vascular beds in the ex ovo chicken embryonic model of angiogenesis were assessed. Gas barrier films were used to modulate local oxygen levels in the chorioallantoic membrane (CAM), a respiratory vascular network, and the area vasculosa (AV) on the yolk sac, a typical peripheral …

A Quadruplex Real-Time Pcr Assay For The Rapid Detection And Differentiation Of The Burkholderia Pseudomallei Complex: B. Mallei, B. Pseudomallei, And B. Thailandensis, Chinn-Woan Lowe

Theses and Dissertations

Methods for the rapid detection and differentiation of the Burkholderia pseudomallei complex comprising B. pseudomallei, B. mallei, and B. thailandensis, have been the topic of recent research due to the high degree of phenotypic and genotypic similarities of these species. B. pseudomallei and B. mallei are the causative agents of melioidosis and glanders, respectively. B. pseudomallei and B. mallei are recognized by the CDC as tier 1 select agents. Although B. thailandensis is generally avirulent in mammals, this species displays very similar phenotypic characteristics to that of B. pseudomallei. Optimal identification of these species remains problematic, …

Heart Rate Variability As A Moderator Of Trauma Writing Outcomes, Alison Eonta

Theses and Dissertations

Writing about personal traumatic experiences is associated with beneficial effects on physical and psychological symptoms compared with writing about emotionally neutral events. However, not everyone benefits from trauma writing to the same extent. The present study hypothesized that the effectiveness of trauma writing may be moderated by emotion regulation, as indexed by respiratory sinus arrhythmia (RSA). Research also shows that greater physiological reactivity is predictive of better trauma writing outcomes. Given the importance of physiological output in emotional processing, response training was developed and found to increase appropriate physiological reactivity. Because higher RSA is thought to indicate a more flexible …

Size-Based Separation Of Bioparticles Using Planar Nanofluidic Devices, Jie Xuan

Theses and Dissertations

Nanofluidic devices are structures having at least one dimension in the submicron range, which is of the same order of magnitude as the sizes of biomolecules and bioparticles such as proteins and viruses. As a result, size-selective separations are important applications for nanofluidics. Well-defined micro or nano device structures fabricated via micromachining have greatly reduced sample consumption and enabled separations in a parallel fashion, promising significant speed and resolution advantages over conventional size separation techniques, such as gel electrophoresis and size exclusion chromatography. In collaboration with others, I have developed a size separation method using nanofluidic devices consisting of an …

Protein Kinase C Dependent Inhibition Of Kir3.2 (Girk2) Channel Activity And Its Molecular Determinants, Scott Adney

Theses and Dissertations

Inwardly rectifying potassium (Kir) channels are critically important for regulating resting membrane potential in excitable cells, a job underscored by the severe pathophysiology associated with channel dysfunction. While all Kir channels require the activating lipid PIP2, many of these channels have diverse modulatory factors that couple to PIP2-dependent gating. Channels in the Kir3 (GIRK) family, in particular, have several co-activating elements, including G-protein betagamma subunits, ethanol, and sodium. During stimulation of Gq-coupled receptors, downstream activation of Protein Kinase C can phosphorylate and inhibit Kir3 channels, yet the mechanism of inhibition and phosphorylation sites are incompletely understood. We took a combined …

Sensor-Driven Hierarchical Path Planning For Unmanned Aerial Vehicles Using Canonical Tasks And Sensors, Spencer James Clark

Theses and Dissertations

Unmanned Aerial Vehicles (UAVs) are increasingly becoming economical platforms for carrying a variety of sensors. Building flight plans that place sensors properly, temporally and spatially, is difficult. The goal of sensor-driven planning is to automatically generate flight plans based on desired sensor placement and temporal constraints. We propose a simple taxonomy of UAV-enabled sensors, identify a set of generic sensor tasks, and argue that many real-world tasks can be represented by the taxonomy. We present a hierarchical sensor-driven flight planning system capable of generating 2D flights that satisfy desired sensor placement and complex timing and dependency constraints. The system makes …

A Comparison Of Attention Bias Paradigms Related To Insomnia, Nathaniel Maloney

Theses and Dissertations

Attentional bias to sleep related stimuli by poor sleepers has been reported as a factor in initiating and maintaining sleep disturbance. In this study, three paradigms (Flicker, Posner, and Stroop) were used in comparison to further investigate the role of attentional biases in Primary Insomnia. Participants (n=117) were split into good sleepers and poor sleepers using a median split with the Pittsburgh Insomnia Rating Scale (PIRS). Using the Center for Epidemiologic Studies Depression Scale (CES-D) as the covariate, two-way mixed modality ANCOVAs were conducted for each paradigm revealing no significant differences in word type, quality of sleep, or the interaction …

The Effects Of Neurotensin On The Rat Distal Colon, Rick Chainani

Theses and Dissertations

The enteric nervous system controls the gut through the release of specific neurotransmitter and neuromodulators at specific sites such as mucosal secretory cell or smooth muscle cell. In the present study, we have examined the response to one of these neurohumoral agents, Neurotensin, in the rat distal colon. Neurotensin is a paracrine and endocrine modulator of the digestive tract. Even though these effects have been seen in colonic preparations, there are very few functional studies of the effects of Neurotensin in the rat colon, especially the distal colon. In the current study we propose the following hypothesis that Neurotensin will …

Are Your Parents To Blame? Disentangling Temperament, Parenting Style, And Schemas In The Development Of Depressive Symptoms, Jenny Delorme

Theses and Dissertations

This study examined Jeffrey Young's Schema Theory (1990; 2003) and its influence in understanding the etiological factors related to depressive symptoms. It was hypothesized that there would be significant interactions between parenting and temperament traits. It was also hypothesized that The Defectiveness and Insufficient Self Control EMSs would mediate the relationship between the significant parenting/temperament interactions and depressive symptoms. Participants were administered the Young Schema Questionnaire Short form-3 to measure the endorsement of schemas; the Beck Depression Inventory to measure symptoms of depression; the Young Parenting Inventory to measure perceived parenting; and the Adult Temperament Questionnaire to measure temperament traits. …

Locus Of Control And Quality Of Life In Individuals With Schizotypy, Rachael Paulbeck

Theses and Dissertations

The present study collected data from 200 undergraduate students (106 male, 94 female) and sought to examine the relationship between levels of stress, cognitive processes related to stress management (Locus of Control- LOC; Sense of Coherence; SOC), and quality of life (QOL) across varying levels of risk for developing schizophrenia. Results indicated that high-risk individuals are significantly more likely to have an external locus of control (F=4.121, p=.018) supporting hypothesis 1. Counter to our second hypothesis, locus of control was not directly associated with QOL. However, LOC was correlated with both stress and level of risk for schizophrenia which (in …

The Role Of Outer Membrane Protein A In Anaplasma Marginale Cellular Invasion And Its Potential As A Cross-Protective Antigen, Sarvani Emani

Theses and Dissertations

Anaplasma phagocytophilum and A. marginale are the etiologic agents of human granulocytic anaplasmosis and bovine anaplasmosis, respectively. Both diseases can be severe, even fatal, and protective vaccines for each are lacking. We recently identified A. phagocytophilum outer membrane protein A (ApOmpA) as being critical for cellular invasion and is expressed during infection of mammalian but not tick cells. Disrupting ApOmpA-host cell interactions significantly inhibits A. phagocytophilum entry into host cells. ApOmpA and its A. marginale ortholog, AM854 (A. marginale OmpA; AmOmpA) exhibit 44% amino acid identity. The ApOmpA invasin domain is highly conserved between both proteins. In this study, we …

An Analysis Of The Marketing Tactics Used To Influence Millennial Generation Students In Their Decision To Attend A Two-Year Or A Four-Year College, Andrea Stanton

Theses and Dissertations

This study will attempt to evaluate the effectiveness of college recruitment marketing tactics used to influence Millennial Generation students, ages 17 to 22 years old, in their decision to attend either a two-year or a four-year college. Changing demographics and projections indicate a decline in the number of high school graduates within the next three to five years. This shift translates into fewer college applications and more competition among institutions of higher learning making it easier for most students to get accepted into college. The researcher will use focus groups and a survey to examine the perception of community colleges …

The Effects Of Planting Techniques On Maize Grain Yield And Silage Production, Tyler D. Kaufman

Theses and Dissertations

This thesis is a comprehensive analysis of the effects of seed orientation, row direction and planting population on grain yield, kernel composition, and silage yield and composition. Producers are striving to increase profit by efficiently using their available land area to maximize crop production. However, little research is available testing the effect of seed orientation on grain or silage production. Quantifying the impacts of seed orientation and row direction on grain and silage is essential for maximum crop production. Dissemination of the results should benefit grain and silage producer.

Two experiments were conducted to evaluate the effects of manipulating seed …

The Role Of Nitric Oxide Dysregulation In Tumor Maintenance, Christopher Rabender

Theses and Dissertations

The inflammatory nature of the tumor microenvironment provides a cytokine and chemokine rich proliferative environment. Much of the responsibility of this environment is due to the production of Reactive Oxygen Species (ROS). These studies examined the proliferative rich tumor environment from a new perspective of Nitric Oxide Synthase (NOS) dysregulation. NOS’s have the ability to become uncoupled and generate superoxide in lieu of nitric oxide (NO). A requirement of NOS for the production of NO is the cofactor tetrahydrobiopterin (BH4) and when it is missing NOS becomes uncoupled and turns into a peroxynitrite synthase. Here I demonstrate that NOS is …

Homophonic Translation, Appositional Writing, And The Monster, Ryan Landry Clark

Theses and Dissertations

This dissertation features a combination of critical and creative work

exploring the ethics of appropriative writing and the reparative potential of homophonic translation. The opening essay examines the ethics of appropriation- based poetry and introduces the concept of what I call "appositional writing," a term to describe ethically-minded works of poetry that make use of appropriative writing methods. The next three parts of this dissertation are each appositional writing projects that make use of homophonic translation as the primary method of composition. "Arizona State Bill 1070: An Act" is a homophonic translation of the anti-immigration bill of the same name. …

The Freshman Experience, Stephanie Mannon

Theses and Dissertations

The purpose of this exploratory research was to investigate if change in sexual risk-taking behavior between high school and college can be predicted as a function of the interaction of person and situation traits. College, conceptualized as a backspace (situation) construct, and personality traits (sensation-seeking, neuroticism, extraversion) conducive to deviant behavior were examined. While significant risky behavior was reported for all students (n = 252), hierarchical regression analyses revealed that higher levels of the specified personality traits in combination with on-campus backspace residential status was not predictive of greater change in engagement in sexual risk-taking behavior. Implications for future research …

Formation, Functionalization, Characterization, And Applications Of A Mixed-Mode, Carbon/Diamond-Based, Core-Shell Phase For High Performance Liquid Chromatography, Landon A. Wiest

Theses and Dissertations

My work has focused on a variety of different types of diamond-based, core-shell particles. These particles are formed with inert cores and poly(allylamine)/nanodiamond shells. Their intended purpose is to form an LC stationary phase that is stable from pH 1 – 14 and at elevated temperatures. At the beginning of my studies, the particles that had been made in the Linford laboratory were pH stable, but irregular and had poor mechanical stability. Since that time, I have worked to improve the particles by using more spherical zirconia and carbon cores, and I have improved their mechanical stability via chemical crosslinking …

Patient Perceptions Of Shared Decision Making: What Does It Mean And How Does It Affect Patient Outcomes?, Laura Aubree Shay

Theses and Dissertations

Introduction: Shared decision making (SDM) has been advocated as an optimal approach to medical decision-making. Yet, little is known about how patients perceive SDM and whether patient-defined SDM is associated with patient outcomes. Methods: This three-manuscript dissertation used a mixed-methods approach including a systematic literature review and both qualitative and quantitative research methods. The aims were to: (1) systematically review the patient outcomes studied in relation to SDM and identify under what measurement contexts SDM is associated with which types of patient outcomes; (2) use in-depth, qualitative interviews to develop a conceptual model of patient-defined SDM and compare this to …

Self-Assembled Dna Origami Templates For The Fabrication Of Electronic Nanostructures, Elisabeth Pound Gates

Theses and Dissertations

An important goal of nanoscience is the self-assembly of nanoscale building blocks into complex nanostructures. DNA is an important and versatile building block for nanostructures because of its small size, predictable base pairing, and numerous sequence possibilities. I use DNA origami to design and fold DNA into predesigned shapes, to assemble thin, branched DNA nanostructures as templates for nanoscale metal features. Using a PCR-based scaffold strand generation procedure, several wire-like nanostructures with varying scaffold lengths were assembled. In addition, more complex prototype circuit element structures were designed and assembled, demonstrating the utility of this technique in creating complex templates. My …

Administrator Leadership And Content Knowledge: Effects On Literacy Achievement On Male Students Grades Four Through Eight, Kay Calvert

Theses and Dissertations

The purpose of this study was to explore the relationship of principal characteristics and school socio-economic factors and their relationship to student achievement as measured by the literacy portion of the Arkansas state standardized test, with a particular interest in male students. The study was undertaken to contribute to the existing knowledge base regarding principal knowledge of reading and its relationship to literacy. The first four independent variables of principal characteristics were defined by the School Capacity model (Newman, King, & Youngs, 2000), and included four domains: (a) possesses instructional skills and knowledge about reading, (b) focuses on program coherence, …

The Relationship Between Perceived Organizational Support And Teachers' Sense Of Efficacy In Regular And Alternative Schools, Jonathan Kyle Knight

Theses and Dissertations

The investigator in the study compared traditional middle and high school teachers to alterative teachers regarding the perceived organizational support, self-efficacy, and retention. The purpose of the study was to determine if there was a difference in the level of self-efficacy between alternative school teachers and traditional school teachers. The researcher also examined the relationships between Perceived Organization Support and teacher self-efficacy between alternative school teachers and traditional school teachers. It was also examined whether alternative teachers perceived their own self-efficacy the same as traditional teachers. The research design was a quantitative approach, using one survey for traditional and alternative …