Digital Commons Network^™

A Bioecological Approach To Understanding The Interaction Of Environmental Stress And Genetic Susceptibility In Influencing Cortisol And Blood Pressure In African American Adults, Sandra Marie Coulon

Theses and Dissertations

African Americans are disproportionately affected by high blood pressure, a precursor to cardiovascular disease. Bioecological, biomedical, and gene-environment interaction theories were integrated to test the impact of environmental stress and genetic susceptibility on stress-related outcomes, including waking cortisol, perceived stress, and blood pressure in African-American adults. The primary aim of the study was to investigate the effects of neighborhood socioeconomic status (SES), neighborhood satisfaction, and neighborhood collective efficacy on waking cortisol, perceived stress, and blood pressure and to determine whether genetic risk for increased glucocorticoid receptor sensitivity moderated those relations in a gene-by-environment (GxE) interaction. A secondary aim was to …

Network Analysis And Cluster Detection Using Markov Theory, John William Campbell

Theses and Dissertations

Networks are a vital part of nature and society, yet many aspects of how networks function are still largely unknown. From understanding the internet to biology, chemistry, and physics, networks play a role, but even some of the most basic questions about networks can be difficult to answer. How are two networks alike or different? How do networks within networks form and how can clusters be detected? As networks change with time, how can we monitor those changes? The answers to these questions are vitally important to humans’ understanding of the world. Better understanding of networks allows for things like …

A Three-Dimensional In Vitromodel Of Atherogenesis, Pin Hsuan Chang

Theses and Dissertations

Atherosclerosis is the narrowing of arteries caused by accumulation of cholesterol, calcium and other cellular debris in the inner arterial wall. While extensive studies have been focusing on the inflammatory mechanisms of the disease, pathological and experimental evidence has shown that regions of disturbed flow are susceptible to the atherogenesis. To further understand the relationship between hemodynamics and atherogenesis, this research aims to generate a dynamic, 3D in vitro model of atherogenesis. This will allow for the investigation of specific cellular and molecular mechanisms of plaque formation that will pave the way for new therapies for the disease. A novel …

Facilitating Embodied Instruction: Classroom Teachers’ Experiences With Drama-Based Pedagogy, Peter B. Duffy

Theses and Dissertations

There is much anecdotal evidence to suggest that drama-based pedagogies significantly contribute to improved teaching and learning. Furthermore, there is important research that demonstrates the impact on teachers when they learn about neuroscience. This research project developed six professional development sessions for six elementary school teachers to provide training on both drama-based instruction and research on embodiment. Combining these two fields presented a problem. Arts-based research is qualitative in nature where cognitive science research depends on post-positivistic assumptions of reliability and trust-worthiness. Missing from these standard measures of research (fMRI studies, STROOP Tests, reaction time tests), however, is the impact …

Using Genetic Algorithm To Solve Median Problem And Phylogenetic Inference, Nan Gao

Theses and Dissertations

Genome rearrangement analysis has attracted a lot of attentions in phylogenetic com- putation and comparative genomics. Solving the median problems based on various distance definitions has been a focus as it provides the building blocks for maximum parsimony analysis of phylogeny and ancestral genomes. The Median Problem (MP) has been proved to be NP-hard and although there are several exact or heuristic al- gorithms available, these methods all are difficulty to compute distant three genomes containing high evolution events. Such as current approaches, MGR[1] and GRAPPA [2], are restricted on small collections of genomes and low-resolution gene order data of …

Prediction Of Coronary Heart Disease Within The Aerobics Center Longitudinal Study Population, Jennifer Carol Gander

Theses and Dissertations

This dissertation is a compilation of three studies that were conducted to better 1) Further validate a thoroughly tested Framingham Risk Score (FRS) on a unique cohort with comprehensive measures available, 2) Update and improve the predictability of the FRS through the addition of cardiorespiratory fitness (CRF) while resolving limitations in previous studies, and 3) Assess the predictability of non-exercise estimated CRF (e-CRF) and FRS on CHD. A manuscript was generated for each study utilizing data from the Aerobics Center Longitudinal Study.

To validate the FRS, a multivariable Cox Proportional Hazard Model was used to determine the association between FRS …

Investigation Of The Response And Repair Of Replication, Kathryn Brady

Theses and Dissertations

When a mammalian cell suffers DNA damage, DNA damage signaling responses and repair pathways are invoked. The phosphorylation of histone variant H2AX (γH2AX) and of replication protein A (pRPA) are two well-documented damage signals, marking double strand breaks and stalled replication forks, respectively. Inhibitors of thymidylate synthase (TS) and ribonucleotide reductase (RNR) are chemotherapeutics that act by depriving cells of the deoxynucleotides necessary for DNA synthesis, which causes damage. The response and repair pathways activated by these chemotherapeutics have been studied for a number of years but there remain unanswered questions as to how cancer cells perceive this damage, the …

Role Of Allergic Sensitization, Filaggrin Variants, And Dna, Ali H. Ziyab

Theses and Dissertations

Background: Allergic disorders, including eczema, asthma, and rhinitis, have emerged as a global public health concern due to their elevated prevalence and the associated clinical morbidity. Environmental, immunologic, and genetic factors have been implicated in the pathogenesis of allergic disorders. Allergic sensitization (representing deviated immune responses) and filaggrin gene (FLG) variants (leading to dysfunctional epidermal barrier) have shown to be common predisposing factors in the development of allergic disorders. However, there is a lack of knowledge on their joint effects on the development of single and multiple (coexistence) allergic disorders. More recently, epigenetic mechanisms, such as DNA methylation, have emerged …

Examining Correlates Of Correctional Officer Risk Perceptions And Decision-Making, Frank Valentino Ferdik

Theses and Dissertations

Developing a more complete understanding of the dangers and risks present within correctional environments can enhance prison safety. Research has revealed, however, that existing prison-based risk assessment and management instruments suffer considerable deficiencies, including failing to account for contextual factors that influence inmate misconduct, and basing generalizations off small sample sizes. Several studies have shown that correctional officers are often very accurate when it comes to the risk assessment and management of prison-based dangers. To expand this literature, the current study collected survey data from a statewide sample of maximum security correctional officers. Ultimately the objectives of this dissertation were …

Integrating Biomedical And Psychosocial Approaches To Study Pain In Pediatric Sickle Cell Disease, Alyssa M. Schlenz

Theses and Dissertations

Sickle cell disease (SCD) is a group of inherited blood disorders characterized by recurrent pain. A distinctive obstacle to managing pain in this condition is the substantial heterogeneity of outcomes observed in children, including the rate, intensity, duration, and extent of disability from pain. Previous studies have largely examined this heterogeneity by focusing on either biomedical or psychosocial approaches to this condition rather than pursuing an integrated approach that is consistent with modern conceptualizations of pain. In addition, few studies have examining genetic heterogeneity in pediatric SCD, which remains one of the only strategies for establishing a preventative approach to …

The Effects Of Exercise Training On Cognitive Reserve And Cognitive Function In Healthy Older Women, Katie Marie Becofsky

Theses and Dissertations

Cognitive reserve theory suggests that physical activity may protect individuals from cognitive decline. At the brain level, greater cognitive reserve may manifest as greater neural network efficiency. Our purpose was to investigate 1) whether participation in a 16-week walking program increased brain efficiency, and 2) whether increased brain efficiency correlated with change in fitness and task performance. Our secondary purpose was to investigate whether exercise training improved performance on a battery of cognitive tasks, particularly executive functioning performance. Seventeen healthy but sedentary women aged 60-75 years participated in a supervised walking program; eighteen women served as a non-randomized control group. …

Carboxylate Selective Lanthanide Imprinted Polymer Sensors For Sensor Array, Yang Xu

Theses and Dissertations

A europium-based molecularly imprinted polymer (MIP) was developed and characterized, which combined the highly luminescent properties of europium and the recognition properties of molecularly imprinted polymers. The europium-based MIPs were used as selective sensors for different types of carboxylates. The ultimate goal of this work was to develop a europium-based sensor array to differentiate carboxylates anions. The polymer was synthesized by using a europium–salen complex as the monomer and ethylene glycol dimethacrylate (EGDMA) as the crosslinker. First, a europium containing non-imprinted polymer (NIP) and a series of europium containing MIPs imprinted with acetate, benzoate and phenylacetate were synthesized by free …

Effects Of Individual Phenotypic Variation On Predator-Prey Relationships Of Xanthid Crabs In North Inlet Estuary, South Carolina, Benjamin J. Toscano

Theses and Dissertations

Ecological communities and the biological interactions that regulate community structure are notoriously complex. To make these systems more tractable, ecologists traditionally measure and model communities at the population level, treating individuals as functionally equivalent. While this approach has yielded tremendous insight into the factors governing communities, it remains unclear whether accounting for individual-level variation could improve our capacity to predict the responses of communities to perturbation, a major goal in the midst of unprecedented rates of environmental change.

The objective of this study was to examine the magnitude of individual-level phenotypic variation in predatory crabs (family Xanthidae), and the effects …

Insights Into The Evolutional Adaptations And Functional Switching Mechanism In Dual Function Hemoglobin/Dehaloperoxidase (Dhp) And Modeling Of Cytochrome P450 Active Site With H93g Myoglobin Cavity Mutant, Shengfang Sun

Theses and Dissertations

The coelomic hemoglobin, dehaloperoxidase (DHP), from the sea worm Amphitrite ornata, is a dual function oxygen-binding heme protein that also possesses a significant peroxidase activity. As evolved from an ancient oxygen carrier globin, several structural adaptations have enabled DHP to evolutionally gain significantly enhanced peroxidase capability for self-protection while only minimally compromising its primary function as an O2 carrier. To elucidate these adaptations, the peroxidase activities and O2 affinities of several DHP and sperm whale myoglobin (Mb) mutants have been prepared. Several heme environmental structural factors that regulate the dual functions of DHP have been found, providing insight into how …

Probing Heteroatom-Containing Substrate Oxidation In P450-Cam For Non-Ferryl Reactive Intermediates And Heme Iron Coordination Structural Analysis Using Magnetic Circular Dichroism Spectroscopy, Anuja Ratnakar Modi

Theses and Dissertations

Cytochrome P450 (P450 or CYP) catalysis involves the oxygenation of organic compounds via a series of catalytic intermediates, namely, the ferric-peroxo, ferric-hydroperoxo, Compound I (Cpd I), and FeIII(H2O2) intermediates. The general consensus is that the Cpd I intermediate is the most reactive species in the reaction cycle, especially when the reaction involves hydrocarbon hydroxylation. Other than Cpd I, there is a multitude of evidence, both experimental as well as theoretical, supporting the involvement of other intermediates in various types of oxidation reactions. In part I of this work, the multiple oxidant hypothesis of P450 catalysis has been probed using P450-CAM …

Understanding The Effect Of Contaminants From Bop Materials On Pemfc Electrode: Ex-Situ Studies, Mayukhee Das

Theses and Dissertations

Off-the-shelf Balance of Plant (BOP) materials for proton exchange membrane fuel cell (PEMFC) systems may be more economical than custom-prepared materials. However, they pose a higher risk of decreased performance of a PEMFC by contaminating the electrodes. This dissertation contributes to the understanding of the mechanisms of electrode contamination by studying the effect of leachate extracts and model compounds from structural plastics and assembly aids that may be used as BOP materials. The effect of contamination was investigated by measuring the decrease in electrochemical surface area (ECA) of the Pt/C catalyst and the change in the oxygen reduction reaction (ORR) …

Transforming The Culture Of The Stem Disciplines: A Multiple Case Study Of Successful Strategies For Inclusive Excellence, Telesia Evon Davis

Theses and Dissertations

In spite of the myriad initiatives promoting diversity and inclusion in science, technology, engineering, and math (STEM) disciplines at colleges and universities across the country and the increased funding for these types of initiatives, colleges and universities have experienced modest gains and, in some cases, slight declines in the graduation rates of women and underrepresented minorities (URMs). In addressing this issue, some institutions rely on traditional or “deficit” approaches that place primary responsibility on students for their success in the STEM fields. A growing number of institutions, however, embrace the concept of inclusive excellence, which is an assumption that underrepresented …

The Impact Of Race On Strickland Claims In Federal Courts In The South, Wyatt Gibson

Theses and Dissertations

The primary goal of this study was to examine the legal and extralegal factors that lead to positive outcome Strickland claims. Specifically, the initial purpose of the research was to test whether a defendant’s race affects his/her likelihood of receiving a positive outcome Strickland claim in the South. Prior literature has indicated that black defendants are more likely to receive the death penalty than white defendants, but this study did not find that race is a significant factor in determining the likelihood of a positive outcome Strickland claim in Southern circuits. Of the 207 Strickland claims studied across the Fourth, …

Imperative Risky Business: A Compelling Case For Using Critical Literacy And Social Justice Curricula To Combat Heteronormativity And Homophobia, Candace Anne-Heather Lett

Theses and Dissertations

Recent research documents the oppressive school conditions for Lesbian, Gay Bixsexual, Queer, and Questioning (LGBTQQ) and non-gender conforming students, which can be detrimental to their academic achievement, as well as their physical and psychological well-being. This study explored ways that negative portrayals of LGBTQQ youth and homophobic and heteronormative attitudes might be altered through the incorporation of LGBTQQ texts and critical discussions surrounding the texts, media stereotypes, heteronormativity, heterosexual privilege, and gender in a high school Public Speaking class. The purpose of this study was to investigate ways that curriculum in a high school Public Speaking class might utilize these …

The Effectiveness Of Local Anesthetic Infiltration Into A Surgical Wound Prior To Surgical Closure, Kevin J. Leblanc

Theses and Dissertations

With 73 million surgical procedures annually, acute post-operative pain management is critical to improve patient outcomes and reduce health care costs. Local anesthetic (LA) infiltration prior to surgical incision closure is a frequently used technique in the operating room. This thesis tested the hypothesis that the use of post-incisional infiltration of local anesthetics into surgical incisions will reduce post-operative pain as evidenced by assessing the reduction in post-operative opioids and the Visual Analog Scale (VAS). The first phase of this study was to examine the available literature to determine what evidence existed as to the effectiveness of local anesthetics infiltrated …

Effects Of Hiv-1 Tat Protein On Structure, Function And Trafficking Of The Dopamine Transporter, Narasimha Murty Midde

Theses and Dissertations

The alarming rise in HIV-1 associated neurocognitive disorder (HAND) is, at least in part, associated with HIV-1 viral proteins shed from infected macrophages, including transactivator of transcription (Tat) despite the success of anti-retroviral therapies. The dopamine (DA) system is greatly involved in the progression of HAND and is influenced by psychostimulants like cocaine. The DA transporter (DAT), a key regulator of neurocognitive functions, is a major molecular target for both Tat and cocaine. Our lab previously reported that exposure to Tat decreases DA uptake through allosteric regulation and alters cocaine binding sites in DAT.

In this research project the hypothesis …

Association Of Serum Immunoglobulin G (Igg) Antibodies Against Periodontal Bacteria In Type 2 Diabetes And Pre-Diabetes And Cardiovascular Risk Factors, Deepika Shrestha

Theses and Dissertations

Periodontal disease is an inflammatory disease caused by polybacterial infection and inflammation plays key role that associates periodontal disease with different systemic diseases including diabetes and cardiovascular diseases. The main objective of this study is to evaluate and explore the association of serum IgG antibodies in four distinct clusters that were formed empirically from species specific 19 periodontal antibody titers with T2DM and pre-diabetes adjusting for known confounders such as age, sex, race-ethnicity, income to poverty ratio, education, smoking and drinking alcohol, BMI, WC, physical activity, missing teeth, dentist visits and other nutritional factors.

From the first aim, we formed …

Potential Stormwater Runoff Reductions By Incorporating Low Impact Development: Rocky Branch Watershed, Columbia, Sc, Brett Sexton

Theses and Dissertations

Urbanization and increasing population are primary sources of water degradation, increased flood risks, and channel morphological instability. Impervious surface areas increase with urbanization and result in decreased infiltration capactities, increased stormwater runoff, and more rapid stormwater delivery, which in turn increase flood magnitudes and frequencies. Low impact development (LID) can provide volumetric detention storage to reduce flooding. This thesis reviews types of LID, their attributes and limitations, and examines how effectively they can abstract storm runoff volumes, reduce peak discharge, and delay stormwater arrival times for relatively frequent storm events in the Rocky Branch Watershed (RBW).

The RBW is 50% …

Do Americans’ Perceptions Of The Prevalence Of Prejudice Impact Their Racial Policy Preferences? Investigating Meta-Stereotypes As A Potential Causal Mechanism, Alexandra Reckendorf

Theses and Dissertations

Racial discrimination, though more subtle than in the past, is still an enduring presence in 21st century America. Whether looking at education, health care, the workforce, housing/lending practices, or the criminal justice system, studies routinely confirm that racial prejudice and discrimination persist despite claims of a “post-racial” America. Yet, despite the perseverance of racial prejudice and discrimination, policies correcting racial injustice remain contentious, either failing to receive the requisite support to pass reforms or receiving backlash from the public. This project explores meta-stereotypes in the Black and white communities, and highlights meta-stereotypes’ potential impact when determining why some individuals support …

Ghosts Of The Horseshoe: A Mobilization Of A Critical Interactive, Richard Lee Walker

Theses and Dissertations

Critical Interactives (CIs) are designed to harness the voluntary, reality-bending excitement of discovery as afforded by play, but to do so in the context of rules that mobilize procedural rhetoric to instantiate critical awareness. Critical interactives are not just about improving lives through code or education; rather, they establish a methodology for generating more aesthetic and reflective interactive experiences. To grasp more fully the logic underpinning CIs, we need to understand the powerful nature of interactivity and outline how such interactivity involves a notion of ethics, i.e., a way of living, in and through media practice.

Ghosts of the Horseshoe …

An Investigation Of Mitochondrial Bioenergetics And The Turnover Of Succinated Proteins In The Adipocyte During Diabetes, Ross Tanis

Theses and Dissertations

We previously identified the chemical modification S-(2-succino)cysteine (2SC), which is formed when the Krebs cycle metabolite fumarate reacts with protein cysteine residues, also termed protein succination. Protein succination is increased in the adipose tissue of ob/ob and db/db mice in vivo and in 3T3-L1 adipocytes grown in high glucose in vitro. The increase in 2SC in the 3T3-L1 adipocyte occurs as a direct result of glucotoxicity and increased mitochondrial stress. We have shown that uncoupling agents, which lower mitochondrial stress, prevent the increase in succinated proteins.

In this study we examined the relationship between increased succination and mitochondrial bioenergetics in …

Geochemical Analysis Of Eolian Fluxes During The Transition From Greenhouse To Icehouse Conditions In Equatorial Pacific., Daniel Nicholas Miller

Theses and Dissertations

To assess the atmospheric conditions during times of differing pole to equator thermal gradients through the middle Eocene to early Oligocene, 42 to 30 Ma, samples from deep-sea sediment cores U1331, U1332, U1333 from IODP Expedition 320/321 in the eastern equatorial Pacific (EEP) have been chemically leached to isolate the operationally defined eolian dust (ODED; <63 μm fraction). The ODED was analyzed for neodymium (Nd) isotopic compositions and rare earth element (REE) concentrations in order to determine the provenance of the ODED fraction and to elucidate the environmental conditions (e.g. predominant wind patterns) during deposition over the interval of study. The variations in the ODED fraction are expected to be controlled by changes in sources delivered via eolian input. However, negative cerium anomalies in the Post Achaean Australian Shale (PAAS) normalized rare earth element profiles of the ODED show a biasing geochemical seawater phase to the ODED signature. It is found that εNd values and cerium anomalies (Ce/Ce*) of ODED are consistent with fossil fish teeth values that record bottom water signatures from the same location and time period until ~36-37 Ma; around the Priabonian/Bartonian boundary marked by increased thermal gradients due to the emplacement of high elevation glaciers on Antarctica (Scher et. al., 2014). Moreover, the more negative cerium anomalies correlate to more radiogenic εNd values. The geochemical seawater signature in the ODED samples is believed to be caused by a change in sedimentary phase regulated by the spatial paleoposition of the sites relative to the biological high productivity zone and/or the depth of the CCD. Subsequently these regulators on the sedimentary phases of the ODED were investigated for biasing the geochemical signatures of the ODED, assumed to be changes in sources thereby provenance. Together, the Nd and REE results from the study temporally indicate that the ODED fraction may be a mixing between two sources, that of a biasing biogenic and/or authigenic source that records seawater conditions, and that of an older, less radiogenic continental source, inferred as Asian loess, as an increased thermal gradient prevails over the course of the study. Further research needs to be performed to make more robust and conclusive linkages between sources, mixing of sources, and atmospheric eolian delivery patterns, highlighting the necessity for a better understanding of atmospheric conditions during important climatic events throughout the history of the Earth.

Nitrate Uptake In Central Illinois Streams: A Comparison Along A Transient Storage Gradient, Kristen Lynn Theesfeld

Theses and Dissertations

Transient storage can be influenced by channel morphology. Anthropogenic activities can alter stream morphology by straightening channels, lining channels, or even restoration of previously altered channels. This study focused on whether relative transient storage (RTS) was related to stream type - modified lined, modified unlined, or natural. The study further sought to determine if a greater RTS is correlated to a decrease in percent nitrate-N.

Eight (8) sites, encompassing three (3) different stream types - modified lines, modified unlined, and natural - were studied. Chloride and nitrate tracers were added to streams, and samples were analyzed for tracer concentrations and …

Using Ipads In Teaching Vocabulary For Students With Learning Disabilities, Kellie Smith

Theses and Dissertations

The purpose of this study was to examine the effects of using iPad to improve vocabulary skills for students with learning disabilities. The participants were 2nd grade students placed in a learning disability resource room. Baseline data was collected using traditional methods during vocabulary intrusion for the first three weeks. During intervention, the iPad was integrated in vocabulary instruction by adapting iPad app, Vocabulary City. The findings indicate students understanding, recognition, and implementation of vocabulary words increased when iPad app technology was implemented.

Synthesis Of 4'-Ester Resveratrol Analogues, Chromium Trioxide Oxidation Of Terpenes, And Synthesis Of Mimics Of (-)-Englerin A, Mark Jeffrey Acerson

Theses and Dissertations

4’ -ester analogues of resveratrol were synthesized using reaction conditions developed to produce mono-ester products in the presence of two other unprotected phenols. Basic conditions were employed to deprotonate the most acidic 4’ phenol followed by addition of anhydrides or acid chlorides to give the ester product. The reaction favored 4’-ester formation in polar aprotic solvents with DMSO being the optimal solvent.

(—)-Englerin A is a guaiane-type sesquiterpene containing two ester side chains. Mimics of (—)-englerin A were proposed that retained the ester side chains while replacing the non-polar core with less complicated structures. These proposed mimic cores would maintain …