Digital Commons Network^™

Genetic Relative-Finder Services: A Qualitative Study Of Consumer Experiences, Sarah A. Sciba

Theses and Dissertations

Background: Genetic genealogy is an emergent topic in sociology. To date, over 41 million individuals worldwide have tested with the top four direct-to-consumer genetic testing (DTCGT) companies: AncestryDNA, FamilyTreeDNA, 23andMe, and MyHeritageDNA (Glynn 2022). These tests provide consumers with ethnicity estimates, a list of DNA cousin matches, or both. Despite the popularity of DTCGT, there is a lack of data on consumer experiences with genetic relative-finder services (GRF) in the sociological literature. Methodology: I conducted exploratory, qualitative research in the form of in-depth interviews with eight adult women who have used GRF. I gathered data about their motivations for participation …

Identification Of Pharmacophore Modifications That Enhance Bmp-Receptor Agonism, An Investigation To Understand The Development Of Potential Therapeutic Interventions, Maleka T. Stewart

Theses and Dissertations

Bone morphogenetic proteins (BMPs) are the largest subfamily of the Transforming Growth Factor (TGF)-ß superfamily. BMPs are renowned for their pivotal role in cellular functions such as cell growth, apoptosis, and differentiation. Yet, BMPs have also been attributed to the maintenance of adult tissue homeostasis. BMPs elicit physiological function at the cell membrane via a heterotetrameric complex composed of two Type I and two Type II serine-threonine kinase BMP receptors. Activation of BMP transmembrane receptors results in activation of either canonical (Smad-dependent) and/or non-canonical (Smad-independent) signaling. Given the physiological importance of this intricate communication between receptor activation and induction of …

Next-Gen Device For Uniform Cellular Migration Assay Execution, Nicholas M. Bittner

Theses and Dissertations

Cellular migration assays, particularly scratch assays, are pivotal in biomedical research for cell motility, wound healing, and metastasis. However, conventional scratch assays suffer from variability due to operator dependence, non-uniform scratch geometry, and a modified microenvironment that impacts reproducibility. This thesis presents the development and validation of a low-cost, 3D-printed device designed to standardize cellular migration assays. The device utilizes a spring mechanism and a biocompatible elastomer to create a uniform cellular exclusion zone without harming the surrounding cells. Through iterative prototyping and testing with MCF10A cell lines, the device demonstrated a significantly lower average standard deviation of length compared …

Synthesis And Characterization Of Additive Manufactured Functional Composites For Biomedical Applications, Alexander Thorne

Theses and Dissertations

This dissertation investigates the synthesis and characterization of 3D printed polymer composites, with engineered porosity and both natural and synthetic additives, using fused deposition modeling (FDM) and selective laser sintering (SLS) for potential biomedical applications. The structural, mechanical, tribological, and surface behavior of these composites were evaluated with respect to their suitability for orthopedic load-bearing implants and scaffolds. In the first phase, PEEK-based composites reinforced with 10 or 20 wt% carbon fibers or 20 wt% E-glass fibers were fabricated using FDM with varying infill densities of 30-100% to introduce porosity. Results revealed that loading additives, infill densities, and porosity play …

Leveraging Distributed Semantics From Deep Learning Architectures For Literature-Based Discovery, Clint A. Cuffy

Theses and Dissertations

Literature-based discovery (LBD) is a scientific process that introduces methods to automatically identify novel insights between non-interacting sets of literature. To date, numerous statistical and machine learning-based methods have been applied in the biomedical domain to find treatments for diseases such as Raynaud's disease, Parkinson's disease, and Multiple Sclerosis. However, the lack of standardized practices and creation of bespoke methodologies produces a scenario where the adoption of LBD remains challenging in real-world systems. Our work addresses these concerns through the improvement of five critical areas: 1) error propagation within LBD's a priori dependent tasks, 2) exploring the integration of modern …

Beyond Homogeneity: Exploring Causal Heterogeneity In Psychopathology, Philip B. Vinh

Theses and Dissertations

Traditional models in psychiatric research often impose assumptions of causal homogeneity, treating population-level associations as reflective of uniform underlying mechanisms. This dissertation challenges that assumption by introducing statistical and machine learning frameworks designed to detect and model causal heterogeneity in the development of psychopathology. Central to this approach is the advancement of finite mixture structural equation modeling (FM-SEM) to identify latent subgroups characterized by distinct, and sometimes opposing, causal pathways.

The dissertation comprises three integrated empirical studies. The first introduces mixDoC, a finite mixture extension of the classical Direction of Causation (DoC) model applied to twin data, enabling the detection …

Persistence Of Southwestern White Pine (Pinus Strobiformis) In An Ever-Changing Environment, Shruti Shrivastava

Theses and Dissertations

Abstract

Local adaptation is known to occur in multiple tree species, with climate as a main force for it occurring, indicating adaptation and changes occurring in spatially and temporally changing climates. With increased climate change, the question now is what future persistence may appear as, and by what magnitude trait mismatch may occur. This is observed in southwestern white pine (Pinus strobiformis) for drought-response traits: resistance (RT), recovery (RC), and resilience (RS). Using previously collected data, the genetic and phenotypic diversity were analyzed. Using that information, the trait values for individuals where only genetic data was known was …

Diversification Of Avifauna In The Darién Highland Region: Ecological Niche Modeling, Phylogeography And Morphology To Explore Speciation In The Montane Species Myadestes Coloratus, Chlorospingus Tacarcunae And Margarornis Bellulus., Jorge L. Garzon Vasquez Mrs

Theses and Dissertations

Montane regions in the Neotropics are hotspots of endemism and biodiversity, yet their species are especially vulnerable to the impacts of climate change and geographic isolation. This thesis investigates the evolutionary and ecological dynamics of three bird species endemic to the Darién Highlands of eastern Panama: Myadestes coloratus, Chlorospingus tacarcunae, and Margarornis bellulus. Through an integrative approach combining ecological niche modeling, morphometric analysis, and genomic data from ultraconserved elements (UCEs) and mitochondrial DNA, the study explores the historical biogeography, current distribution patterns, population structure, and phenotypic differentiation of these montane taxa. Ecological niche models revealed that suitable habitat …

Mctr3 Mitigates Cigarette Smoke-Induced Pulmonary Inflammation And Damage, Yu Par Aung Myo

Theses and Dissertations

Smoking is deleterious to our lungs and can precipitate in a multitude of diseases, including chronic obstructive pulmonary disease (COPD). COPD is primarily caused by smoking cigarettes and is a major public health issue. Current therapies only treat symptoms without halting or reversing disease progression, thus highlighting a large unmet need to develop new therapies. We address this gap by investigating maresin conjugate in tissue regeneration 3 (MCTR3) which is a newly discovered specialized pro-resoling mediator (SPM) that has the novel property of promoting tissue repair and regeneration in addition to being anti-inflammatory and pro-resolving. For the first time, we …

The Role Of Context Sensitivity On The Association Between Racial Discrimination And Emotional Dysfunction Among Black American Adolescents, Summer C. Bryant

Theses and Dissertations

Black adolescents are at an increased risk of experiencing racial discrimination due to a developmental period marked by increased time away from parents and caregivers and increased time with peers (Fisher et al., 2000). Empirical research has shown that Black adolescents’ experiences of racial discrimination are associated with emotion dysfunction (Benner et al., 2018; Seaton et al., 2009; Sellers et al., 2006). Although previous research has identified racial factors such as racial socialization and racial identity as protective factors by reducing the impact of racial discrimination on Black adolescents’ emotion dysfunction (Gibson et al., 2021; Neblett et al., 2008), we …

Modeling The Impacts Of The Current And Projected Temperatures On Spongy Moth Population Dynamics, Adrienne B. Spring

Theses and Dissertations

The spongy moth (Lymantria dispar) is an invasive forest pest that has caused significant ecological damage across the United States. Its invasion front is shaped by a number of factors, including temperature in both the northern and southern regions. With ongoing climate change, areas that were previously uninhabitable may become increasingly favorable for moth population establishment and expansion, while other areas may experience thermal stress limiting persistence. This study develops a temperature-driven population model to analyze how temperature affects the spongy moth population dynamics along the invasion front. This model incorporates temperature effects on fecundity, stage-specific survival rates, …

Plza Rna Chaperone Activity In Borreliella Burgdorferi Is Modulated By C-Di-Gmp, Dhara T. Patel

Theses and Dissertations

Lyme disease (LD) is the most common arthropod-borne disease in North America and is caused by the spirochete, Borreliella burgdorferi. LD spirochetes are maintained in an enzootic cycle of Ixodes species ticks and a vertebrate reservoir. The secondary messenger molecule c-di-GMP plays a crucial role in adaptive responses during the enzootic cycle. PlzA is the sole c-di-GMP-binding protein identified to date that is encoded by all Borreliella burgdorferi isolates. PlzA has both c-di-GMP-dependent and c-di-GMP-independent functions and is required to complete the enzootic cycle. The full range of PlzA regulatory activities has yet to be fully defined. We determined …

Tate Speech: Radicalizing Rhetoric And Multi-Level Marketing Recruitment Strategies In The Manosphere, Carey J. Priebe

Theses and Dissertations

My study aims to uncover the discursive elements behind the gendered recruitment strategies that influencers in the manosphere use to pipeline men and boys into their multi-level marketing (MLM) companies. In the manosphere, male supremacy is presented as desirable and subversive, and MLMs take advantage of emotional and economic instability. My study examines the Twitter (X) account of the popular manosphere influencer Andrew Tate as I trace his rhetoric along a three-pronged gendered recruitment framework, a framework originally developed around women selling essential oils online and funneling their followers into radical alt-right politics. What are the similarities and differences in …

Functional Analysis Of Ligand Binding Domain 2 Of Outer Surface Protein C Of Borreliella Burgdorferi, Gavin Z. Chambers

Theses and Dissertations

Lyme disease, caused by the spirochete Borreliella burgdorferi, is the most common vector-borne infection in North America. A critical determinant of early mammalian infection is outer surface protein C (OspC), a highly variable lipoprotein that is indispensable for spirochete survival but whose precise function remains unresolved. OspC has been proposed to mediate diverse roles, including complement evasion, adhesion, tissue dissemination, and antiphagocytic activity, supporting the view that it is a multifunctional virulence factor. Structural studies have identified two putative ligand-binding domains (LBD1 and LBD2), with LBD2 forming a solvent-exposed crown at the membrane-distal end of the OspC dimer.

To …

Mental Health Trends Among African American High School Students, Alexis Heard

Theses and Dissertations

Adolescent mental health is a major public health issue in the U.S., with African American high school students facing a rapidly worsening crisis. While all youth show increasing rates of depression, anxiety, and suicidality, African American adolescents are at greater risk due to systemic racism, socioeconomic inequality, community violence, and limited access to culturally responsive care. Few national studies have examined mental health trends among this group. This study (1) examines 2011–2021 trends in persistent sadness/hopelessness, suicidal ideation, suicide planning, and suicide attempts among African American high school students; (2) explores gender differences; and (3) compares these patterns to national …

The Relationship Of Preschool Variables To Kindergarten Early Literacy Skills, Michele Davis Thomason

Theses and Dissertations

This dissertation examines the impact of Pre-K experiences on kindergarten early literacy skills in Alabama. A convergent parallel mixed-methods research design was utilized to analyze assessment data from My Teaching Strategies AlaKiDS and Renaissance STAR Early Literacy for kindergarten students in a rural Alabama district. The students were divided into three groups for comparison: students who attended Alabama First Class Pre-K, those in private programs using the Abeka curriculum, and students with no formal Pre-K experience. The dissertation utilized Vygotsky’s constructivist theory to assess the influence of early educational environments on the cognitive development through scaffolding and the Zone of …

Assessing Electronic Nicotine Delivery System Abuse Liability Among People Who Smoke Cigarettes: Impact Of Device Characteristics, Nicotine Flux, And Flavor, Elizabeth Oluwapelumi Ogunleye

Theses and Dissertations

This clinical laboratory study utilizes subjective and behavioral economic measures to assess the abuse liability of own-brand cigarettes and six currently authorized ENDS products, examining how device characteristics, nicotine flux, and liquid flavor influence outcomes among adults who smoke cigarettes. Using a Latin-square-ordered, within-subject design, adults who smoke cigarettes (N = 30; N = 20 preferred menthol cigarettes) attended four clinical sessions: one to evaluate their own-brand cigarette and three to assess six NJOY ENDS products: 2.4% nicotine pods (~58 µg/s; tobacco and menthol flavor), 6% nicotine disposable products (~61-65 µg/s; tobacco and menthol flavor), and 5% nicotine pods (~118-123 …

Acute Effects Of Electronic Nicotine Delivery System Liquid Nicotine Form And Sweetener Among People Who Use Inhaled Tobacco Products, Mary A. Carrico

Theses and Dissertations

Research on tobacco product standards to limit the abuse liability of electronic nicotine delivery systems (ENDS) has primarily focused on nicotine concentration and flavor. Other liquid characteristics, such as protonated nicotine ratio and sweetener additives, are less understood but may serve as regulatory targets. This clinical laboratory study examined the effects of ENDS protonated nicotine ratio and sweetener presence on nicotine delivery, use behavior, and subjective effects.

Thirteen participants completed four sessions that varied by protonated nicotine ratio (0:100 vs. 40:60 freebase to monoprotonated nicotine) and sweetener status (sweetened with 3 mg/mL ethyl maltol vs. unsweetened). Each session included a …

Doves And Symbiotic Bacteria: Whole-Genome Assembly And Phylogenomic Inference, Mariam Topchyan

Theses and Dissertations

Part 1

I examined a potential source of variation in phylogenetic inference, investigating whether handling single-nucleotide polymorphisms (SNPs) through different methods during reference-guided genome assembly of pigeon and dove species impacts phylogenetic reconstruction and inference. Specifically, I created a custom consensus base calling tool that handles allelic variation through two different approaches, either ignoring heterozygous sites by denoting them as an ambiguous character “N,” or using a pseudo-random choice between supported base variants. Through reference-guided assembly of 108 dove species, I generated two datasets of alignments using both consensus calling methods, created two trees from both datasets via supermatrix and …

Improving Telehealth Education And Training: A Needs Assessment Of Healthcare Providers And Trainees, Conor Duffy

Theses and Dissertations

Telehealth utilization has increased dramatically since the start of the COVID-19 pandemic. There are many positive impacts of this shift to telehealth, including increasing access to care and reducing patient burden. However, healthcare providers report several consistent challenges that limit their ability to deliver telehealth, such as effective communication, technological difficulties, and difficulty performing physical examinations. To address these concerns, it is necessary to integrate telehealth education and training into healthcare professional training programs and healthcare systems. While many institutions have implemented telehealth education and training since the start of the pandemic, there is little consistency in the content, duration, …

Maternal Postpartum Depression And Affect Incongruence: Exploring Early Developmental Pathways To Emotion Dysregulation, Laura E. Boylan

Theses and Dissertations

Emotion dysregulation, or responses to emotions that do not meet the needs of the situational demands or social standards, has been noted as an early risk factor for later psychopathology. Maternal postpartum depression (PPD) has been shown to increase children’s risk of developing maladaptive emotion regulation (ER) strategies. One potential mechanism linking PPD to these outcomes is affect incongruence, or a mismatch between biological and emotional responses to stress. While such incongruence may be adaptive in older individuals who consciously mask their internal stress (e.g., suppressing biological arousal while appearing calms), it may be maladaptive in infants and young children …

Mutual Realm, Tyna Ontko

Theses and Dissertations

mutual realm is a container for my many meandering thoughts on speculative futures, present-day-truth-telling, and the everyday construction of histories. Informed by systems of belief and power that humans have created to grapple with the unknown, the writing illustrates how storytelling builds worlds, assigning value to things by inscribing them with desire. In parts fact and fantasy, sculpture is considered as a form of sorting, making known the ways in which my experience of living between publics whose methods for demarcating boundaries, communally agreed upon superstitions, and algorithms for assigning meaning have marked my unconscious and affect my daily trajectories. …

Strange World, Brooklin Grantz

Theses and Dissertations

My thesis work explores the intersections of masculine and feminine labors as they relate to the structure of domestic space. I utilize materials and processes akin to craft, construction and architecture, and domicile upkeep, noting what is shared between gendered and polarized fields while exploring uses of the grid in various instances as a structure to adhere to and antagonize: a binary. Highlighting the queerness and fluidity of materials and the similarities between seemingly disparate processes, I relate to the objects I make and alter through my own gender fluidity. I do not see masculinity or femininity as autonomous: the …

Imagine Being Free: Decolonizing Actor Training In University And Conservatory Programs, Hope R. Ward

Theses and Dissertations

While blackface is considered the first proper form of American theater, I want to consider how the auction of enslaved Africans functioned as the original form of American theater. The auction block acted as a kind of interactive performance in which enslaved bodies were surveilled and evaluated as a kind of spectacle. In many ways, the auction block inaugurated White viewing practices of the Black body that reverberated in the day-to-day practices of slavery and subsequent visual media like the refashioning of blackface minstrel tropes within contemporary entertainment (plays, television, film). In Imagine Being Free: Decolonizing Actor Training in University …

Supervisor Support And Job Satisfaction: The Mediating Roles Of Work-Life Balance And Perceived Employability With Gender As A Moderator, Tori Lomelino

Theses and Dissertations

This study explored the relationship between supervisor support and job satisfaction, offering work-life balance and perceived employability as key mechanisms through which this relationship occurs. It also explored gender differences in the relationship between work-life balance and job satisfaction, and perceived employability and job satisfaction. Participants were full-time employees in the U.S, recruited via Prolific, who completed an online survey. While supervisor support was found to have positively influenced job satisfaction through perceived employability, the role of work-life balance was less clear. Interaction effects including gender were not significant. This study highlights the significant role of perceived employability as a …

Dynamic Expression Of Transposable Elements Contributes To Molecular And Behavioral Adaptations To Cocaine, Gabriella M. Silva

Theses and Dissertations

The comorbidity of substance use disorder (SUDs) — specifically cocaine use disorder (CUD)— with anxiety and social deficit disorders complicates effective long-term treatment. More research is needed to understand the brain-region and cell type-specific mechanisms underlying these disorders to better address treatment gaps. This dissertation investigates the role of the transcription factor of interest ZFP189, a Krüppel-associated box (KRAB) zinc finger protein (ZFP), in governing pro-social behavior and the development of CUD across interconnected brain regions. We show that ZFP189 directly regulates transposable elements (TEs), repetitive DNA sequences capable of mobilizing and reinserting into the genome, which leads to subsequent …

Physiological Responses And Plant Trait Interactions With Coastal Dune And Swale Development, Emily C. Riffe

Theses and Dissertations

Vegetation is crucial to coastal ecosystems experiencing climate change and storm-induced disturbance. Plant species interact with the physical environment and are considered “ecosystem engineers.” Sand dunes and barrier islands are at the forefront of climate change impacts as they are barriers that protect landward habitat and communities. Nature based solutions are utilized to protect infrastructure on developed coastlines. Understanding how dominant plant species interact with and alter the physical environment enhances our understanding of the mechanisms of change and consequences to the system. The goal of this research was to quantify physiological and functional trait differences in dominant species found …

Post Opera, Suzy Lykins

Theses and Dissertations

Post Opera is a spell and work of embodied research which explores medicalization, desire, labor, and belief. Through methods of performance, sound, text and installation, I facilitate encounters of trans-femininity, spiritual power and connectivity. An opening wound, a set of psychic scalpels, sutures and bandages prepare this text for your consumption, take care to digest her well.

A Comparison Of Involved And Uninvolved College Students' Stress And Coping During Covid-19, Abbie Alsene

Theses and Dissertations

In this study, I compare the stress and coping of involved students and uninvolved students at liberal arts colleges during COVID-19. The study seeks to understand if there are differences between the two student populations. Three research questions guide the study. First, how has COVID-19 affected the stress levels of involved students compared to uninvolved students at liberal arts colleges? Second, how do involved students cope with their stress related to COVID-19 compared to uninvolved students at liberal arts colleges? And lastly, what is the relationship between how involved college students view COVID-19 and their levels of stress in comparison …

Investigation Of The Human Temporal Styloid Process As A Bony Indicator Of Speech, Sommer A. Martin

Theses and Dissertations

The temporal styloid process in humans serves as an attachment point for the styloglossus, stylohyoid, and stylopharyngeus, which are involved in tongue movement and deglutition. I hypothesized that the length of the ossified styloid process in humans is the result of selection for increased musculature that would support tongue movements involved in speech. This hypothesis assumes that the relative length of the ossified temporal styloid process is unique to humans. A comparative analysis of the presence or absence of the temporal styloid process was conducted on ten genera of Old-World primates, and it was found that the length of this …