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Larval Environments Affect The Implementation Of The Sterile Insect Technique On Aedes Mosquitoes, Katherine G. Evans
Larval Environments Affect The Implementation Of The Sterile Insect Technique On Aedes Mosquitoes, Katherine G. Evans
Theses and Dissertations
Sterile Insect Technique (SIT) is an effective method for controlling pests and has been increasing in popularity to control Aedes mosquitoes. SIT involves inundating an area with sterilized male mosquitoes to reduce egg hatch and ultimately the number of wild adults. Previous research indicates that enacting mortality early in the larval life of Aedes mosquitoes can result in counter-productive effects, due to reduced competition among developing larvae. I hypothesize that the effectiveness of SIT at reducing adult populations is modified by densitydependent mortality and density-dependent growth among larvae. I performed two field-to-lab studies and developed an individual-based model to test …
“Money Is Just A Tool, Not The Goal”: Examining Messages That Parents Tell Their Children About Money, Hannah Delorto
“Money Is Just A Tool, Not The Goal”: Examining Messages That Parents Tell Their Children About Money, Hannah Delorto
Theses and Dissertations
Parental communication plays a crucial role in a young person's development of financial knowledge and behaviors, such as budgeting, saving, investing, retirement preparation, consumer skills, and avoiding excessive debt. This research focuses on emerging young adults aged 18-25, who are a vulnerable group of spenders. Participants shared memorable messages they received about money and rated the messages for positive or negative affect. Then, they answered questions measuring their financial literacy, perceived financial wellbeing, attitude about the importance of money. Four major themes were represented in the data of memorable messages: daily habits, long-term goals, family finances, and the meaning of …
Boys Build, Girls Glam: An Examination Of Adult Language Use In Informal Steam Education Settings, Alexandra Horton
Boys Build, Girls Glam: An Examination Of Adult Language Use In Informal Steam Education Settings, Alexandra Horton
Theses and Dissertations
Research on STEAM education has primarily focused on formal education settings such as schools, with more research needed in informal or non-traditional education settings. As children frequently engage with informal and non-traditional education settings, it is important to understand how we can transmit information in these settings to bolster and further the learning process and increase engagement. STEAM fields have been plagued by racial and gender inequities that have led to these groups being marginalized and excluded (Blair et al., 2017; Eaton et al., 2020; Martin, 2018; Martin & Phillips, 2019; Miller et al., 2018; Newall et al., 2018, Sheffield …
The Experiences Of Black Male Dei Administrators At Historically White Institutions, Kwame R. Patterson Jr
The Experiences Of Black Male Dei Administrators At Historically White Institutions, Kwame R. Patterson Jr
Theses and Dissertations
This study examined the lived experiences of Black male Diversity, Equity, and Inclusion (DEI) administrators at Historically White Institutions (HWIs) in higher education. Drawing from Critical Race Theory (CRT) and the Campus Racial Climate Model, this research utilized a qualitative narrative inquiry to understand how Black male DEI administrators experience and navigate their roles, as well as the support systems they rely on to overcome barriers encountered at HWIs. I conducted interviews with 11 Black male DEI administrators from various HWIs. Three major themes emerged from the analysis: (1) Limited Legacy: What am I here for?, which highlights the unique …
Analyzing Factors Influencing Employee Turnover In Tech Companies: A Predictive Modeling Approach, Shinjon Ghosh
Analyzing Factors Influencing Employee Turnover In Tech Companies: A Predictive Modeling Approach, Shinjon Ghosh
Theses and Dissertations
Employee turnover poses substantial challenges for technology firms, and understanding its key drivers through predictive modeling is essential for developing effective retention strategies. This study investigates factors influencing employee turnover in technology companies by implementing a predictive modeling approach on the IBM HR Analytics Employee Attrition dataset. The research aims were identifying key factors contributing to employee attrition, developing predictive models to forecast turnover risk, and analyzing interactions among significant predictors. By examining a range of features, the results highlight significant variables (Over Time, Monthly Income, Marital Status, etc.) of attrition and offer actionable insights for developing targeted employee retention …
Exploring Nitrate Reduction In A Saturated Riparian Buffer Through A Three-Dimensional Reactive Contaminant Transport Model In An Agricultural Area, Mcclean County Central Illinois, Franklin O. Ijigade
Exploring Nitrate Reduction In A Saturated Riparian Buffer Through A Three-Dimensional Reactive Contaminant Transport Model In An Agricultural Area, Mcclean County Central Illinois, Franklin O. Ijigade
Theses and Dissertations
Saturated Riparian Buffers (SRBs) are engineered systems designed to remove nitrate and other pollutants from subsurface agricultural drainage water before it reaches a stream. This study utilizes a high-resolution coupled flow and reactive transport model to explore nitrate reduction and transformation in an SRB in glacial sediment. The influence of material heterogeneity on nitrate reduction and the effectiveness of the SRB was investigated. The study was conducted in an SRB located next to and receiving tile drainage water that ultimately drains into a 3rd order stream (T3 stream) in central Illinois, USA. A three-dimensional steady groundwater flow model was …
Application Of Floating Wetlands To Improve Urban Stream Quality, Daniel Chukwunonso Chukwudi
Application Of Floating Wetlands To Improve Urban Stream Quality, Daniel Chukwunonso Chukwudi
Theses and Dissertations
Urban streams, impacted by urbanization, often exhibit degraded water quality due to elevated nutrient levels, a phenomenon known as urban stream syndrome. This study evaluates the effectiveness of a floating wetland (FW) system in reducing nitrate as nitrogen (NO₃-N) and phosphate (PO₄³⁻) concentrations in a side canal of the Chicago River’s North Branch, adjacent to Goose Island, Chicago, IL, from 2018 to 2023. Using a five-year dataset, including new sampling from May to December 2023, the research addresses two questions: (1) Do FWs lower NO₃-N and PO₄³⁻ concentrations in urban streams? and (2) Do FWs exhibit seasonal differences in nutrient …
A Novel Silver-Ruthenium-Based Antimicrobial Kills Gram-Negative Bacteria Through Oxidative Stress-Induced Macromolecular Damage, Patrick O. Tawiah
A Novel Silver-Ruthenium-Based Antimicrobial Kills Gram-Negative Bacteria Through Oxidative Stress-Induced Macromolecular Damage, Patrick O. Tawiah
Theses and Dissertations
The rise in drug-resistant bacteria, together with the decline in antibiotic development, requires new strategies for infectious disease control. Gram-negative pathogens are particularly challenging to combat due to their outer membrane. This study highlights the effectiveness of the silver-containing antimicrobial AGXX® against the Gram-negative bacterium Escherichia coli. AGXX® is a promising silver coating that presumably kills bacteria through the generation of reactive oxygen species (ROS). However, neither AGXX®’s mode of action is fully understood, nor have its effects on Gram-negative bacteria or bacterial response and defense mechanisms towards AGXX® been studied in detail. Here, I report that the bactericidal …
Theoretical Frameworks Used To Conceptualize The Intersection Of Deafness, Emotional/Behavioral Disabilities, And Language In The Literature: A Scoping Review, Jeremy M. Peterson
Theoretical Frameworks Used To Conceptualize The Intersection Of Deafness, Emotional/Behavioral Disabilities, And Language In The Literature: A Scoping Review, Jeremy M. Peterson
Theses and Dissertations
The prevalence rate for emotional/behavioral disabilities (EBD) among d/Deaf and hard of hearing (d/DHH) students has consistently been reported as statistically elevated in the literature, but the exact reason for this high prevalence has not been determined. Factors such as diagnostic shadowing, language proficiency, and difficulties in interfamilial communication have been identified; however, recent research suggests that language deprivation may also be a contributing factor. The intersection of language deprivation and EBD among d/DHH students has been studied by numerous researchers, but, to date, no one has explored how these topics are conceptualized together in the literature. As such, I …
The Impact Of Ableist Microaggressions On The Supervisory Relationship And The Influence Of Burnout And Self-Efficacy, Berenice Contreras
The Impact Of Ableist Microaggressions On The Supervisory Relationship And The Influence Of Burnout And Self-Efficacy, Berenice Contreras
Theses and Dissertations
Disabled graduate students often report experiences of ableist microaggressions in their training environment, as disability-affirming practices tend to be overlooked in higher education institutions (Kattari et al., 2019; Lund et al., 2020). Ableist microaggressions are verbal, nonverbal, and/or environmental messages that relay derogatory messages toward individuals with disabilities and normalize everyday forms of discrimination enacted toward them (Annamma et al., 2013; Kattari et al., 2019). Studies demonstrate that disabled graduate students in health psychology graduate programs report higher exposure to ableist experiences during pre internship training and supervision compared to other stages of graduate training (Lund et al., 2014; Lund …
Normalizing Delinquency And Risky Behaviors In Adolescents: An Application Of Neutralization Theory To Parents, Audrey Cheatham
Normalizing Delinquency And Risky Behaviors In Adolescents: An Application Of Neutralization Theory To Parents, Audrey Cheatham
Theses and Dissertations
Sykes and Matza’s Neutralization theory (1957) was originally developed to explain juvenile involvement in delinquency, with extensions of this theory being used to understand crime in other offending groups. Although neutralization theory has been widely applied to offenders, far less research has examined how neutralizations may be used by non-offending groups. This study applies Sykes and Matza’s framework to parents to explore whether neutralizations are being employed to justify risk taking or delinquent behavior among their adolescent children. This study also examines how parents differentiate risky behavior from delinquent behavior, as well as their attitude towards seven major risk factors …
A Qualitative Inquiry Into The (In)Visibility Of Black Women Leaders In Social Work, Jere Murry
A Qualitative Inquiry Into The (In)Visibility Of Black Women Leaders In Social Work, Jere Murry
Theses and Dissertations
Black women are vital contributors and innovators within the American workforce yet remain underrepresented in executive leadership roles in most employment settings. Despite the contributions Black women have made, our continued marginalization is a direct result of systemic oppression and institutionalized racism, which persistently deny Black women opportunities for leadership ascension (Beckwith et al., 2016; Collins, 2000; Crenshaw et al., 1995; Wilkerson, 2020). This qualitative narrative approach explores the stories of Black women leaders employed in the child welfare and foster care system, illuminating the challenges, including systemic barriers, limited mentorship, and inequitable career advancement practices. This study examines the …
The Role And Impact Of Forensic Evidence In The Prosecution Of Homicide Cases In The U.S., Afrah Dakhilallah A. Altubait
The Role And Impact Of Forensic Evidence In The Prosecution Of Homicide Cases In The U.S., Afrah Dakhilallah A. Altubait
Theses and Dissertations
The role of forensic evidence in the criminal justice process is an important topic that is significant from both theoretical and practical perspectives. The present study is dedicated to a critical analysis of the role of this evidence in the U.S. criminal justice process with a focus on its significance in homicide cases. Specifically, this dissertation examines the implications of using DNA and fingerprint evidence for convictions in U.S. homicide cases by analyzing the role of DNA and fingerprint evidence in the prosecution of “hot” and “cold” homicide cases, discussing the main ways in which forensic evidence assists with prosecuting …
Psychopathology, Symptomology, And Treatment In Offspring Of Holocaust Survivors: An Integrative Systematic Review, Lauren Grace Escobar
Psychopathology, Symptomology, And Treatment In Offspring Of Holocaust Survivors: An Integrative Systematic Review, Lauren Grace Escobar
Theses and Dissertations
This systematic review aimed to evaluate the symptomatology, psychopathology, and treatment of offspring of Holocaust survivors (OHS). A comprehensive search was conducted in PsycINFO, PILOTS/PTSD, and Scopus for studies published between 1990 and 2024. Inclusion criteria required peer-reviewed studies, reports, books, literature reviews, and meta-analyses written or translated into English that reported on symptomatology and treatment for OHS. From an initial yield of 2,532 articles, 119 studies met the inclusion criteria. The findings suggest that OHS demonstrate significantly higher levels of symptomatology and psychopathology reported in nine clinical classifications. Psychodynamic treatment was the most commonly found treatment modality for OHS, …
Understanding The Mentorship Experiences Of Underrepresented Minority Stem Leaders, Elias Saade
Understanding The Mentorship Experiences Of Underrepresented Minority Stem Leaders, Elias Saade
Theses and Dissertations
There is a growing need to diversify the science, technology, engineering, and math (STEM) workforce to better address complex societal challenges and drive innovation. However, persistent disparities limit the retention and advancement of underrepresented minorities (URMs) as leaders in STEM. Mentorship has emerged as a promising practice to address these challenges, yet a gap in the literature remains regarding the impact mentorship had on URM STEM leaders’ personal and professional development. This study responds to that gap by exploring the lived experiences of URM STEM leaders and their perceptions of how mentorship shaped their career development, psychosocial growth, and identity …
The Impact Of Covid-19 On Autistic Youth Mental Health, Chase Keyler
The Impact Of Covid-19 On Autistic Youth Mental Health, Chase Keyler
Theses and Dissertations
This systematic literature review explores the experiences of autistic youth during the COVID-19 pandemic and their ongoing adjustment in a post-pandemic world. It focuses on three primary concerns: the impact of the COVID-19 pandemic on ASD youth’s mental health, the factors influencing their adjustment during and after lockdown, and post-pandemic recommendations for clinicians and stakeholders. A comprehensive electronic literature search was conducted across six databases within the EBSCOhost platform. Study selection consisted of three phases: an initial screening (title and keywords), a partial review (title, keywords, and abstract), and a full- text analysis. The results of this systematic review indicate …
Virulence Of Emerging Tick-Borne Pathogens, Borrelia Miyamotoi And Borrelia Mayonii, Dawn Cleveland
Virulence Of Emerging Tick-Borne Pathogens, Borrelia Miyamotoi And Borrelia Mayonii, Dawn Cleveland
Theses and Dissertations
As climate change leads to global expansion of tick habitats, tick borne diseases are becoming more prevalent and widespread, and emerging tickborne pathogens Borrelia miyamotoi and Borrelia mayonii warrant increased study. Both B. miyamotoi and B. mayonii are vectored by the same tick species as B. burgdorferi, the causative agent of Lyme disease, with B. miyamotoi causing a relapsing fever-like disease known as Borrelia miyamotoi disease (BMD), and B. mayonii causing a Lyme disease-like illness with atypical clinical presentations. This dissertation has two aims. The first aim encompasses B. miyamotoi infection and growth, isolation of clonal populations, and characterization of …
The Relationship Between Social Determinants Of Health And Maternal Risk Factors With Severe Maternal Morbidity, Rebecca Smith
The Relationship Between Social Determinants Of Health And Maternal Risk Factors With Severe Maternal Morbidity, Rebecca Smith
Theses and Dissertations
Severe maternal morbidity (SMM) remains a significant public health concern in the United States, with rates rising despite advancements in obstetric care. While extensive research has examined the role of clinical risk factors in SMM, less attention has been given to the influence of social determinants of health (SDOH). Factors such as financial insecurity, food access, housing stability, intimate partner violence, social connectedness, and transportation barriers may contribute to maternal health disparities. This study aims to assess the relationship between SDOH and SMM, evaluating how these non-clinical factors shape maternal health outcomes. Additionally, this research explores the impact of maternal …
High School Counselor Perceptions Of Trauma-Informed Professional Development And Implementation Of Trauma-Informed Practices In Schools, Kimberly Faulkner-Camacho
High School Counselor Perceptions Of Trauma-Informed Professional Development And Implementation Of Trauma-Informed Practices In Schools, Kimberly Faulkner-Camacho
Theses and Dissertations
This non-experimental phenomenological study aimed to delve into school counselors’ perspectives on what motivates them to participate in trauma-informed professional development, the necessary supports for implementing trauma-informed practices in schools (TIPS), and the barriers that impede implementation. Drawing on situated learning theory and adult learning theory, this study sought to enhance the understanding of school counselors’ views on trauma-informed professional development and implementation. These frameworks offered valuable insights into how to support counselors and shed light on existing barriers. The study involved 10 traditional public high school counselors from non-charter schools in Los Angeles County, selected through snowball and purposive …
Implications For Clinical Practice When Working With Latino Families Affected By Pediatric Cancer: An Integrative Systematic Review, Karla Rodas
Theses and Dissertations
The high mortality rates within the Latino population due to childhood cancer and the psychosocial challenges that come with the disease warrants attention from mental health professionals to provide support and address the distress experienced by affected families. The purpose of this integrative systematic review was to identify the psychosocial challenges faced by Latino families navigating childhood cancer, explore the role of cultural resiliency as a coping mechanism, and examine culturally adapted clinical interventions that integrate cultural resilience, promote psychosocial well-being, and improve treatment engagement. A PRISMA flow diagram was used to illustrate the systematic review process, which included 11 …
Incarcerated Youth Returning To Society As Adults: An Examination Of Their Lived Experiences And Challenges, Kimberly R. Lawrance
Incarcerated Youth Returning To Society As Adults: An Examination Of Their Lived Experiences And Challenges, Kimberly R. Lawrance
Theses and Dissertations
When children have been incarcerated, they face collateral consequences that make it nearly impossible to recover from when they return home. Collateral consequences are the adverse effects experienced by incarcerated people and their families often causing restrictions or missed opportunities after interaction with the criminal justice system (Moise, Simonsen, & Kearl, 2023). Collateral consequences can be related to housing, employment, and education, to name a few. These lost opportunities are concerning for youth because most have never held a job or finished high school. Collateral consequences often set them up for failure leading to the revolving doors of jails and …
Exploring The Role Of Transcription Factor Nrf1 In Autophagy And Inhibiting The Nrf1 Bounce-Back Response, Madison A. Ward
Exploring The Role Of Transcription Factor Nrf1 In Autophagy And Inhibiting The Nrf1 Bounce-Back Response, Madison A. Ward
Theses and Dissertations
Cells exposed to proteotoxic stress invoke adaptive responses aimed at restoring proteostasis. Our previous studies have established a firm role for the transcription factor Nuclear factor-erythroid derived-2-related factor-1 (Nrf1) in responding to proteotoxic stress elicited by inhibition of cellular proteasome. Following proteasome inhibition, Nrf1 mediates the synthesis of new proteasomes, thus enabling cells to mitigate proteotoxic stress. Here, we report that under similar circumstances, multiple components of the autophagy-lysosomal pathway (ALP) were transcriptionally upregulated in an Nrf1-dependent fashion, thus providing the cells with an additional route to cope with proteasome insufficiency. In response to proteasome inhibitors, Nrf1-deficient cells exhibited profound …
Lis1 Depletion In Adult Mouse Projection Neurons Causes Neurodegeneration And Severe Neurological Phenotypes, Anne Ventrone
Lis1 Depletion In Adult Mouse Projection Neurons Causes Neurodegeneration And Severe Neurological Phenotypes, Anne Ventrone
Theses and Dissertations
Proper LIS1 function is crucial for human brain development, as mutations in LIS1 lead to lissencephaly, a severe neurodevelopmental disorder caused by defective proliferation, differentiation and migration of neural precursors. Our understanding of LIS1's functions after brain development is limited. LIS1 regulates cytoplasmic dynein 1 (dynein), an essential microtubule motor protein in cell division and migration, but also a cellular transport motor. We previously showed that tamoxifen (TX)-induced global knockout of LIS1 using a Cre/Lox system (Act-Lis1iKO) in young adult mice results in severe neurological defects and rapid lethality. This strongly supports LIS1's critical post- developmental role, but the underlying …
Characterization Of Methylated Histone Modifications During Simian Virus 40 Early Transcription, Jacob Otto Haugen
Characterization Of Methylated Histone Modifications During Simian Virus 40 Early Transcription, Jacob Otto Haugen
Theses and Dissertations
Modifications of histone proteins play an important role in transcription. These modifications regulate how chromatin is compacted by the histone proteins and either made available for transcription or prevents transcription machinery from proceeding. The modifications can be added or removed by histone acetyltransferases and histone methyltransferases for the former, and histone deacetylates and demethylases for the latter. To explore the dynamicity of these modifications we used the simple but effective Simian Virus 40 (SV40) model. SV40 continues to be an important tool in understanding eukaryotic transcription. It experiences similar processes as cellular chromatin, utilizing host proteins to propagate its own …
Assessing Teachers’ Conceptual Leadership Perceptions Of School-Site Administrators As An Approach To Improve Teacher Morale, Darlene C. Landy
Assessing Teachers’ Conceptual Leadership Perceptions Of School-Site Administrators As An Approach To Improve Teacher Morale, Darlene C. Landy
Theses and Dissertations
The purpose of this study was to have teachers complete a self-assessment survey to assess their conceptual belief of the leadership styles of school-site administrators that are conducive to high teacher morals. The questions addressed how teacher morale affected the teachers according to the number of years they had been teaching, their college majors, their highest college degree, their gender, and their ethnicity. The teachers who participated in this study were volunteers who had to be teaching for 2 years or more.
This was a quantitative study that used the Teacher Rapport With Principal dimension of the Purdue Teacher Opinionnaire …
The Biomechanics Of Braking Squat And Split Braking Squat: An Experimental Approach, Ariel Atias
The Biomechanics Of Braking Squat And Split Braking Squat: An Experimental Approach, Ariel Atias
Theses and Dissertations
An experimental study was undertaken to investigate the biomechanics of braking and split braking squat among NCAA jumpers under three incremental loading conditions (BW, BW+50%, BW+100%). Fifteen males (age 20.6±2.61years, weight 80.36±10.52kg, height 183.06±5.22cm) performed both exercises. One-way RMANOVA, Bonferroni post-Hoc tests were conducted independently on each exercise. Braking squat showed 38% reduction in peak vertical ground reaction force from BW to BW+50%, 53% from BW to BW+100% (all p≤0.001); split braking squat, 34% reduction from BW to BW+50%, 63% from BW to BW+100%, 29% from BW+50% to BW+100% (all p≤0.001). Braking squat showed 53% increase in ground contact time …
More Than Players, More Than Coaches: Institutional Agents And Youth Development In Urban And Suburban Minority-Majority Neighborhoods, Rainah N. Folk
More Than Players, More Than Coaches: Institutional Agents And Youth Development In Urban And Suburban Minority-Majority Neighborhoods, Rainah N. Folk
Theses and Dissertations
Institutional agents play a crucial role in shaping youth development. While the institutional agent framework is well established in postsecondary research, less is known about its role during mid-adolescence. Using relational ethnography and responsive interviewing, this study compares an urban and a suburban high school in minority-majority neighborhoods in Cook County, Illinois, to explore how development is influenced through relationships between sport coaches and high school athletes. Athletes in both contexts face significant challenges tied to neighborhood conditions and socioeconomic status. In response, coaches form deeply caring and supportive relationships, though their roles diverge in ways shaped by their urban …
The Development Of A One Pot, Two Reaction Synthetic Preparation Of 1,2,4-Oxadiazole, And Synthesis Of Ataluren, A Novel Drug In The Fight Against Duchenne Muscular Dystrophy. Efforts Towards The Synthesis Of A Potential Protease Inhibitor Against The Main Protease (3clpro) Of Covid-19, Austin J. Carter
Theses and Dissertations
1,2,4-Oxadiazoles are five-membered heterocyclic structures first synthesized by Tiemann and Krüger in 1884. Recent discoveries suggest an array of useful biological properties.In preparation of 1,2,4-oxadiazoles, the most prevalent precursors are amidoximes, which can be created via the reaction of nitriles with hydroxylamine hydrochloride and a base. The amidoximes are reacted with an activated carboxylic acid to yield an acylated intermediate. This intermediate can be exposed to a base to cyclize into the 1,2,4-oxadiazole. This thesis describes the efforts in combining these three steps into a unified one-pot synthesis.
Currently, there are a significant number of commercially available FDA approved and …
The Path From Acceptance To Adoption For Linguistic Innovations Dealing With Gender In The Language Classroom, Juan Carlos Acevedo Fontecha
The Path From Acceptance To Adoption For Linguistic Innovations Dealing With Gender In The Language Classroom, Juan Carlos Acevedo Fontecha
Theses and Dissertations
This dissertation investigates the factors influencing the acceptance, rejection, or adoption of gender inclusive and gender-non-identifying forms among language users. The study draws on responses from 25 students in a linguistic class to evaluate innovations in English and from 23 instructors of Spanish to evaluate innovations in Spanish. Participants took part in a survey designed to evaluate existing forms endorsed by the United Nations, and gender-non-identifying forms, such as the -e morpheme in Spanish or singular they in English, promoted by community-generated guides. Quantitative and qualitative data were analyzed side by side to better understand the participants’ reasoning regarding these …
Site-Specific Modification Of Antibodies Utilizing Microbial Transglutaminase For Improved Immunoassay Performance, Emily Beitello
Site-Specific Modification Of Antibodies Utilizing Microbial Transglutaminase For Improved Immunoassay Performance, Emily Beitello
Theses and Dissertations
Immobilization strategies used for fixing antibodies to solid phase surfaces significantly contribute to the antigen capture capacity and influences the performance and sensitivity of immunoassays. Traditional methodologies result in the random orientation and/or the weak attachment of immobilized antibodies. Different formats of immunoassays scope in applicability to many fields of research and everyday use. Improving the efficiency of immobilizing antibodies in immunoassays is a beneficial endeavor to increase assay sensitivity. Here, we investigated the use of enzyme-mediated conjugation of antibodies to facilitate a highly oriented immobilization. Microbial transglutaminase (mTG) catalyzes the covalent bond formation between a plethora of primary amine …