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Kinetics And Mechanisms Of Accumulation For Liposomal Ciprofloxacin Into Rat Alveolar Macrophages, Sayeed Mossadeq

Theses and Dissertations

The kinetics and mechanism of accumulation for liposomal ciprofloxacin (Lipo-CPFX) into the rat alveolar macrophage NR8383 cells were studied in vitro, in comparison to unformulated ciprofloxacin (CPFX). Upon incubation with CPFX or Lipo-CPFX, cellular drug accumulation was determined from the cell lysates or efflux was from the extracellular media by fluorescence-HPLC. The accumulation for Lipo-CPFX reached the asymptotic values at ≥ 2 hours, which was a result of uptake and efflux. The uptake appeared to be due to liposomes, mediated via cellular energy-independent mechanism like lipid fusion. In contrast, the efflux appeared to be due to ciprofloxacin, partly cellular energy-dependent, …

Calcium/Calmodulin-Dependent Protein Kinase Ii Beta (Camkiiβ): A Regulator Of Oligodendrocyte Maturation And Myelination, Christopher Waggener

Theses and Dissertations

Oligodendrocytes are cells located in the central nervous system (CNS) that are responsible for the production of the lipid rich membrane, myelin. Myelin and the process of making and wrapping myelin around an axon (also known as myelination) are critical for normal development since they ensure proper signal conduction in the vertebrate CNS. The loss or damage of this myelin, which is typically associated with the demyelinating disease multiple sclerosis (MS), is associated with improper axonal protection along with disrupted nerve signaling which can lead to a variety of different debilitating phenotypic responses. It has been shown that there are …

The Role Of The Nucleosome Remodeling And Histone Deacetylase (Nurd) Complex In Fetal Γ-Globin Expression, Maria Amaya

Theses and Dissertations

An understanding of the human fetal to adult hemoglobin switch offers the potential to ameliorate β-type globin gene disorders such as sickle cell anemia and β-thalassemia through activation of the fetal γ-globin gene. Chromatin modifying complexes, including MBD2-NuRD and GATA-1/FOG-1/NuRD play a role in γ-globin gene silencing, and Mi2β (CHD4) is a critical component of NuRD complexes. In the studies presented in Chapter 2, we observed that the absence of MBD2 in a sickle cell mouse model leads to a decrease in the number of sickled cells observed in the peripheral blood, and significantly increases survival in these mice. Although …

Hormone Epimers Regulate Er Stress And Core Regulatory Genes: Network Analysis With Applications To Glioma And Chronic Pressure Ulcers, Thomas L. Shaak

Theses and Dissertations

DHEA has been determined to have medically significant activity and is the parent compound to the more active metabolites; 17α-AED, 17β-AED and 17β-AET, which exhibit strong biological activity that has been attributed to androgenic, estrogenic or anti-glucocorticoid activity in vivo and in vitro. This study compared DHEA, 17α-AED, 17β-AED and 17β-AET for their ability to activate the human AR, ER and GR receptors and determine the relative androgenicity, estrogenicity and glucocorticoid activity. The results show that, at the receptor level, these androstene hormones are weak AR and even weaker ER activators. Direct androstene hormone activation of the human AR, ERα, …

The Metabolic Transitions Regulated By The Estrogen-Related Receptor (Err) In Drosophila Melanogaster, Yan Li

Theses and Dissertations

In multicellular organism, bioenergetic metabolism is strictly regulated toward efficient generation of ATP. However, in certain situations, such as in limiting oxygen or in the rapidly proliferating system like growing juvenile or cancer cells, organisms apply the metabolic strategy that favors the production of biomass (e.g., nucleotides, amino acids, and lipids) over efficiency of ATP generation. The conserved estrogen-related receptors (ERRs) are master regulators in controlling metabolic homeostasis, and good candidates for mediating the metabolic transition induced by hypoxia and development. First, we investigate how dERR influences hypoxic adaptation in Drosophila melanogaster. We find that dERR is required for a …

In Vitro Lung Epithelial Cell Transport And Anti-Inflammatory Activity For Liposomal Ciprofloxacin, Ruba Darweesh

Theses and Dissertations

Liposomal ciprofloxacin (Lipo-CPFX) is being developed for inhalation, with a goal of sustaining the therapeutic activity, compared to unformulated ciprofloxacin (CPFX). However, the kinetics and mechanism of its sustained local lung retention and pharmacological activity are yet to be fully characterized. This project hypothesized that Lipo-CPFX enables slower and sustained lung epithelial transport and uptake, compared to CPFX, thereby producing prolonged local pharmacological actions. The human bronchial epithelial Calu-3 cells were used as monolayers to characterize the kinetics and mechanism of transport and/or uptake, and to assess the effects of such slow kinetics for Lipo-CPFX on its inhibition against lipopolysaccharide …

Role Of The Alzheimer's Amyloid Precursor Protein In High Fat Diet Induced Obesity And Regulating Macrophage Phenotype, Kendra L. Puig

Theses and Dissertations

Amyloid precursor protein (APP) derived amyloid beta peptides have been extensively investigated in Alzheimer's disease pathology of the brain. However, the function of full length APP in the central nervous system remains unclear. Even less is known about the behavior of this ubiquitously expressed protein and it metabolites outside of the central nervous system. Therefore, we sought to broaden our understanding of the expression and function of APP and its proteolytic fragments in specific non-neuronal tissues. Although the majority of research effort is currently focused on neuronal amyloid beta production and its effects on cells, prior work in our lab …

Wendell Berry's Philosophy Of Education: Lessons From Port William, Jane Margaret Schreck

Theses and Dissertations

In the midst of a proclaimed crisis in higher education, in the clamor and clamber to leverage technology for such innovations as mass open online courses and differentiated learning modules, in the speculative frenzy of preparing students for the careers of a fantasy future, and in the swirl of angst about funding accountability and economic relevance, Wendell Berry's philosophy of education declares that the essential element missing from most current discussions and considerations of education is love. As explained in his essays and revealed in his fiction and poetry, Berry's philosophy centers on love as the best animator of learning: …

Targeted Overexpression Of Metallothionein In Endothelial Cells Mitigates Pulmonary Alveolar Basement Membrane Thickening In Transgenic Diabetic Ove Mice: An Unbiased Tem Morphometric Analysis, Holly M. Hewitt

Theses and Dissertations

Damage to small blood vessels in diabetes mellitus (DM) is largely the result of hyperglycemia-driven oxidative stress. This is manifested morphologically by capillary basement membrane (BM) thickening, the hallmark feature of chronic complications of DM. In an effort to demonstrate pulmonary alveolar BM (PABM) thickness increase in DM and to demonstrate for the first time, mitigation of the increase utilizing antioxidant therapy, we crossed the severely diabetic transgenic mouse (OVE) with Jtmt, our recently developed transgenic animal model that overexpresses the antioxidant metallothionein (MT) in endothelial cells. Unbiased TEM morphometry demonstrated that PABM widths in diabetic (OVE) mice were significantly …

Exploring The Differences In Deep Convective Transport Characteristics Between Quasi-Isolated Strong Convection And Mesoscale Convective Systems Using Seasonal Wrf Simulations, Brandon Clifford Bigelbach

Theses and Dissertations

It has been shown in several previous studies that there is a relationship between mesoscale storm type and deep convective mass transport characteristics. For example, a previous simulation study showed that a supercell storm transported significantly more

tracers into the stratosphere than did a multicell storm in an environment with identical thermodynamic structure. We utilize the Weather Research and Forecasting (WRF) model (version 3.2.1) with chemistry to simulate mass transport during the convective season of 2007 in the U.S. Southern Great Plains at convection-resolving scale (2 km). The storms that were resolved in the model were then classified using an …

Determination Of Optimum Polymer Dose For Grand Forks Wastewater Treatment Plant Lagoon Sludge Dewatering, Dipesh Das

Theses and Dissertations

The Grand Forks Waste Water Treatment Plant (GFWWTP) is currently sending its waste activated sludge (WAS) from the activated sludge treatment process to an existing on-site wastewater treatment lagoon which has been in operation since 2003. The plant produces approximately 65,000 gallons of WAS per day. Because of this high level of loading, the existing lagoon system is no longer considered as a treatment option for the produced sludge. The Plant Authority is trying to find a sustainable solution for sludge disposal and for this reason the GFWWTP is interested in introducing screw press system for the dewatering process. As …

Seasonal Resource Selection, Site-Specific Brood Predictors, And Nest Characteristics Of Greater Prairie-Chicken Hens In Northwestern Minnesota, Nathaniel Emery

Theses and Dissertations

Greater prairie-chickens, Tympanuchus cupido pinnatus, are in decline across the majority of their already receding range due to changing land use patterns and habitat fragmentation. The Agassiz Beach Ridge region of northwestern Minnesota harbors one of the only sustained to increasing populations of prairie-chickens in the country due to the conversion of marginal agricultural lands to grasslands and through conservation entities securing tracts of land allowing for habitat stability and dispersal.

Nesting and brood-rearing habitats are the most limiting factors for population sustainability. Hens were marked with radio transmitters on booming grounds and at nest sites discovered by nest-dragging. Their …

Historical Hydroclimatic Change At Theodore Roosevelt National Park: 1895 - 2011, Rhonda Jan Fietzek-Devries

Theses and Dissertations

Although numerous studies have examined hydroclimatic and climatic changes in our national parks, no previous studies have examined historical temperature, precipitation and hydroclimatic changes at Theodore Roosevelt National Park (South Unit). Documenting historical trends in temperature, precipitation and hydroclimatic variables is important for understanding present and future changes in vegetation and wildlife.

I used 117 (1895 - 2011) years of temperature and precipitation data obtained from the PRISM data network to construct the historical climatic water budget time series based upon the Thornthwaite water-budget model. Trend lines and descriptive statistics are used to analyze the monthly, seasonal and annual climatic …

"Oh, Must We Dream Our Dreams And Have Them Too?": Transformation Through Reading In Elizabeth Bishop's "Questions Of Travel," Carol Shields' Swann, And Florian Henckel Von Donnersmarck's The Lives Of Others, Carissa Anne Green

Theses and Dissertations

Literary scholars study how readers interpret texts. Louise Rosenblatt, Wolfgang Iser, and Wayne Booth analyze how readers interact with texts transactionally, as a phenomenology, and as an ethical exchange. This grounding gives a foundation on which to analyze and define the interpretive acts demonstrated by readers. Poststructuralist critics Roland Barthes and Jacques Derrida open up the idea of "text," allowing us to apply our phenomenology of reading beyond words on paper. I apply the work of these and other critics not to the readers of texts but to characters depicted within texts who are readers. These characters confront unfamiliar texts, …

A Sustainable Bio-Solids Management For The Grand Forks Wastewater Treatment Plant, Hasibul Hasan

Theses and Dissertations

The Grand Forks Waste Water Treatment Plant (GFWWTP) is currently sending its waste activated sludge (WAS) from the activated sludge treatment process to an existing on-site wastewater treatment lagoon which has been in operation since 2003. The plant produces approximately 65,000 gallons of WAS per day. Because of this high level of loading, the existing lagoon system is likely to get replaced by a more sustainable treatment option. Several methods were considered and studied thoroughly for this research, and - on site land application shows some potential. After surveying the Municipal Waste Water Treatment Facilities of the five neighboring states …

Exploring The Impact Of Zebra Mussels (Dreissena Polymorpha) On Residental Lakeshore Property Values In Otter Tail And Becker Counties, Minnesota, Spencer Crowley Mcgrew

Theses and Dissertations

Aquatic invasive species are a major problem in Minnesota. Aquatic invasive species disrupt ecosystems, limit lake recreation, and reduce lakeshore property values. They are expensive to manage, and often impossible to eradicate once established. In this study, I am asking this question: What is the economic impact, in terms of residential shoreline property values, of the introduction of the invasive zebra mussel (Dreissena polymorpha) into large and popular freshwater lakes in west central Minnesota?

Past studies comparing lakeshore property values based on environmental factors such as water quality, clarity, lake size, and depth have shown that these environmental amenities are …

This Is Only A Test, Laurel Lynn Perez

Theses and Dissertations

This is Only a Test, a collection of original poems, explores perception: how we view ourselves both in our everyday lives and in our obsessions. My hope is to not necessarily to critique these moments or years in our lives, but to expose them in my aesthetic project, using free verse, form, and invented form to set them against the unexpected. By using and departing from traditional poetic forms I create new ways of reading the content. In writing about themes such as fertility, relationships, sexuality, and violence I look at the places we never meant to be in view …

Epileptic Seizure Detection And Prediction From Electroencephalogram Using Neuro-Fuzzy Algorithms, Ahmed Fazle Rabbi

Theses and Dissertations

This dissertation presents innovative approaches based on fuzzy logic in epileptic seizure detection and prediction from Electroencephalogram (EEG). The fuzzy rule-based algorithms were developed with the aim to improve quality of life of epilepsy patients by utilizing intelligent methods. An adaptive fuzzy logic system was developed to detect seizure onset in a patient specific way. Fuzzy if-then rules were developed to mimic the human reasoning and taking advantage of the combination in spatial-temporal domain. Fuzzy c-means clustering technique was utilized for optimizing the membership functions for varying patterns in the feature domain. In addition, application of the adaptive neuro-fuzzy inference …

A Case Study Of Elementary Teachers' Conceptions Of Environmental Literacy In Relationship To A Tall Grass Prairie Restoration Project, Teresa Jayne Shume

Theses and Dissertations

The purpose of this qualitative case study was to describe seven elementary teachers' conceptions of environmental literacy in relationship to a tall grass prairie restoration project and to explore ways in which the tall grass prairie restoration project for third grade contributed to enhancing educational learning experiences. The research questions were: 1. What are teachers' conceptions of environmental literacy for third grade students? 2. How does the prairie restoration trip contribute to teachers' capacity to teach for environmental literacy of third grade students? 3. What is the pedagogical value of the prairie restoration project? The theoretical frameworks underpinning this study …

Creating The Role Of Iago: An Analysis Of The Pre-Rehearsal Process, Jordan Brent Wolfe

Theses and Dissertations

The process of creating a character entails much more than memorizing a few lines, learning blocking, and repeating them on stage. An important aspect for an actor passionate about improving their craft as an artist comes during the pre-rehearsal process. Before the audience enters the theatre, or the director has taken control of the actors on stage, it is important, as an actor, to begin to develop the many aspects of the character that he or she will bring to the play. This thesis is a character study of Iago, from Othello. The first chapter will explore a textual analysis …

Acetate Increases Brain Purine Metabolism And Glial Cell Culture Fatty Acid Content: Studies Investigating Glyceryl Triacetate's Therapeutic Mechanism Of Action, Dhaval Paresh Bhatt

Theses and Dissertations

Acetate supplementation is anti-inflammatory and neuroprotective in rat models of neuroinflammation and Lyme neuroborreliosis. Oral administration of glyceryl triacetate (GTA) is used to induce acetate supplementation in rodents, which increases plasma acetate and brain acetyl-CoA levels. Acetyl-CoA is a ubiquitous substrate for several biochemical pathways including carbohydrate, protein, and lipid metabolism. Acetate increases the in vivo and in vitro acetylation-state of histones and is associated with altered cytokine balance. However, the effect that acetate supplementation has on brain purine and lipid content remains unknown. Since labeled-acetate is incorporated into the purine and lipid pools, we proposed that acetate supplementation by …

An Investigation Of Gender's Role In Three- And Four-Year-Old Children's Musical Play, Kari Jo O'Keefe

Theses and Dissertations

Young children engage in many activities, with play being the most prominent. Musical play occurs when children freely investigate sound through vocal, instrumental, or other means. Identifying the extent of musical play - and the role that gender plays in musical play - may provide important information about children's natural tendencies.

The purpose of this study was to investigate gender's role in three- and four-year-old children's musical play, and to investigate further the general nature of musical play. A grounded theory methodology was used, and data was gathered from field and individual child observations, and parent and preschool teacher surveys. …

Grassland Songbird Response To Landscape Composition And Vegetation In Northeastern And East-Central North Dakota, Dustin James Vanthuyne

Theses and Dissertations

Grassland songbirds are declining faster and more consistently than other avifauna of North America. This has resulted in many species being placed on state and federal lists as species of concern. These declines can be linked to former and current land use practices which have resulted in an extensive loss of habitat. Natural resource agencies are trying to offset these habitat losses by reconstructing grasslands formerly cropped areas and protecting remaining grassland tracts. Few studies have addressed how the location in the landscape and vegetation composition of these reconstructions affects grassland birds. This thesis describes data collected from 32 locations …

Pregnancy, Sexism, And The Greek System, Taylor Marie Walkky

Theses and Dissertations

Both hostile and benevolent sexism have been found to correlate with attitudes related to pregnancy (Terrell et al, in prep). Benevolent and hostile sexism have both been shown to correlate with attitudes towards sorority members as well (Walkky, unpublished). The goal of the present study was to expand on the knowledge of how sexism relates to pregnancy attitudes, especially how it relates to sorority membership. Participants read a scenario about a college woman, who was either in a sorority or not, who had just discovered that she was pregnant. In the scenario, she chose to either end the pregnancy, continue …

It's In Minnesota's Nature: An Exploration Into Building Social Capital Through Place Branding, Alyssa Michelle Chandler

Theses and Dissertations

Place branding is a growing practice in communities worldwide. While much academic research has been conducted on the subject, the majority is directed toward the study of tourist perceptions. Research on those living in a branded community is limited. Therefore, this study seeks to explore how a place brand could build or strengthen social capital among residents of a location through a shared representation of a community's identity. A case study was conducted in Grand Rapids, Minnesota where 30 residents were interviewed from diverse socio-economic backgrounds. Through the lens of the seven community capitals, discussed in Flora and Flora (2009), …

Epigenetic Patterns: Process-Driven Or Sequence-Driven?, Amanda Gefroh

Theses and Dissertations

Some of the most rapidly-evolving fields of study in the medical and research communities presently are those which investigate our genetic code, the elements and factors that control the expression of our genetic code, as well as fields of study which analyze theories about an organisms' physiology and phenotype from the information that both of the previous topics concomitantly provide. The work of the Milavetz lab, among many others, is striving to fill the gaps in knowledge about epigenetic regulation of biological processes - here by using the well-suited model virus, Simian Virus 40. It has for some time now …

A Country Of Giants, Benjamin Mace Greenlee

Theses and Dissertations

This thesis, A Country of Giants, consists of four short stories which explore the borderland between youthful innocence and adult understanding. This borderland is often considered in "coming of age" tales, a literary genre that investigates the growth of youthful characters. Through my four stories I examine themes including a loss of innocence, claiming one's identity, independence, and sexual understanding. In addition to those themes I present situations where adults lose their experiential understanding, digressing into a more innocent and uninformed state. I tend to write realistic stories, and it is my hope this use of realism develops well-rounded characters …

The Influence Of Television And Film On Interest In Space And Science, Katrina Marie Jackson

Theses and Dissertations

Entertainment media has the great potential to inspire interest in the topics it presents. The purpose of this study is to better understand how entertainment media contributes to people's interests in space and science. There is a huge variety of science communication topics in previous literature, some of which deals with television and film, but very little that specifically study how television and film can inspire interest. A historical review of pioneers in the space industry shows that many were inspired by entertainment media, which at the time consisted of science fiction novels and magazines. In order to explore the …

Inhibition Of The Ysc Type Iii Secretion System Of Yersinia Pestis By Compound D And Interaction Of Type Iii Secretion System Needle Proteins With Host Receptors, Danielle L. Jessen

Theses and Dissertations

The type III secretion system of gram-negative bacterial pathogens is a major virulence factor and functions to modulate host immune responses. Immune modulation occurs in many ways, including direct injection of effector proteins or indirect methods such as the detection of bacterial components by host immune receptors. Knowledge of these immune modulations allows for development of treatment options in an ever-increasing antibiotic-resistance climate. The studies presented here explore both areas of immune modulation. We identify Compound D as a potent inhibitor of the type III secretion system of Yersinia pestis. Through evaluation of effector secretion by bacteria grown in the …

A Mass Spectrometry-Based Method For Quantification Of Human Metallothionein Isoforms And Usefulness In Biomarker Detection Of Cancers And Metal Toxicity, Aaron Andrew Mehus

Theses and Dissertations

Current methods for detecting metallothionein (MT) protein expression lack the specificity to distinguish between all twelve human isoforms. Each, however, can be distinguished by the masses of their acetylated, cysteine-rich, hydrophilic N-terminal tryptic peptides. These properties were exploited to develop a mass spectrometry-based method for their simultaneous quantification.

Human kidney HK-2 epithelial cells expressing recombinant MT-3 were grown in the presence or absence of cadmium. Cytosolic proteins were alkylated with 14N- or 15N-iodoacetamide and digested with trypsin. The N-terminal MT peptides were enriched by two-dimensional liquid chromatography and analyzed by MALDI-TOF/TOF mass spectrometry. Relative expression was measured by combining 14N-labeled …