Digital Commons Network^™

Kinetics Of Atmospheric Reactions Of Biogenic Volatile Organic Compounds: Measurement Of The Rate Constant Ofthujone + Cl· At 296 K And Calculation Ofthe Equilibrium Constant For The Ho2ch2ch2o2· H2o Complex, Marie Coy Killian

Theses and Dissertations

Biogenic volatile organic compounds (VOCs) react with Cl and OH radicals and the resulting radicals combine with oxygen to form peroxy radicals RO2. Organic peroxy radicals can then react with NO to form NO2, a precursor of tropospheric ozone. The work presented here explored the initial reaction between Cl and thujone, a VOC emitted by Great Basin sagebrush. The rate constant for the reaction of thujone + Cl at 296 K was measured with the method of relative rates with FTIR for detection of reactants. LEDs were used to photolyze Cl2 to generate Cl in the reaction cell. Thujone was …

Orthogonal Expression Of Metabolic Pathways, George Howard Mcarthur Iv

Theses and Dissertations

Microbial metabolism can be tailored to meet human specifications, but the degree to which these living systems can be repurposed is still unknown. Artificial biological control strategies are being developed with the goal of enabling the predictable implementation of novel biological functions (e.g., engineered metabolism). This dissertation project contributes genetic tools useful for modulating gene expression levels (extending promoters with UP elements) and isolating transcription and translation of engineered DNA from the endogenous cellular network (expression by orthogonal cellular machinery), which have been demonstrated in Escherichia coli for the production of lycopene, a 40-carbon tetraterpene carotenoid with antioxidant activity and …

Trophies, Plaques, And Rewards: An Application Of Cognitive Evaluation Theory To Volunteer Motivation., John Kim

Theses and Dissertations

Using Cognitive Evaluation Theory, this research evaluated the impact non-monetary extrinsic incentives in the form of department t-shirts had on the intrinsic motivation of Virginia career and volunteer firefighters. Intrinsic motivation was measured using the Work Extrinsic and Intrinsic Motivation Scale (WEIMS). The survey was administered to a randomly selected sample of firefighters. Data was analyzed using multiple regression analysis. Results suggested that the department t-shirt did not have a statistically significant impact on intrinsic motivation of both career and volunteer firefighters. Additionally, this study suggested that volunteer firefighters did not perceive salary as an inequitable reward. This was exhibited …

Ethanol Regulation Of Glucocorticoid Responsive Genes, Blair Costin

Theses and Dissertations

Glucocorticoid hormones modulate acute and chronic behavioral and molecular responses to drugs of abuse including psychostimulants and opioids. Acute ethanol activates the hypothalamic pituitary adrenal (HPA) axis causing the release of adrenal glucocorticoid hormones, but following chronic ethanol the HPA axis is dysregulated in both humans and rodents. Thus, there is growing evidence that glucocorticoids might also modulate behavioral and molecular responses to ethanol. Previous microarray studies in the Miles’ laboratory have shown that the well-known glucocorticoid responsive gene, Serum and Glucocorticoid-regulated Kinase 1, Sgk1, is prominently up regulated by acute ethanol (2 g/kg) in the prefrontal cortex (PFC) of …

Multiple-Instance And One-Class Rule-Based Algorithms, Dat Nguyen

Theses and Dissertations

In this work we developed rule-based algorithms for multiple-instance learning and one-class learning problems, namely, the mi-DS and OneClass-DS algorithms. Multiple-Instance Learning (MIL) is a variation of classical supervised learning where there is a need to classify bags (collection) of instances instead of single instances. The bag is labeled positive if at least one of its instances is positive, otherwise it is negative. One-class learning problem is also known as outlier or novelty detection problem. One-class classifiers are trained on data describing only one class and are used in situations where data from other classes are not available, and also …

The Art Of Perl: How A Scripting Language (Inter)Activated The World Wide Web, Norberto Gomez Jr.

Theses and Dissertations

In 1987, computer programmer and linguist Larry Wall authored the general-purpose, high-level, interpreted, dynamic Unix scripting language, Perl. Borrowing features from C and awk, Perl was originally intended as a scripting language for text-processing. However, with the rising popularity of the Internet and the advent of Tim Berners-Lee’s World Wide Web (Web), in the 1990s, Perl soon became the glue-language for the Internet, due in large part to its relationship to the Hypertext Transfer Protocol (HTTP) and the Common Gateway Interface (CGI). Perl was the go-to language for on the fly program writing and coding, gaining accolades from the likes …

Characterization Of The Neurotrophic Factor Brain-Derived Neurotrophic Factor (Bdnf) In Intestinal Smooth Muscle Cells, Mohammad Alqudah

Theses and Dissertations

Brain-derived neurotrophic factor (BDNF) belongs to the neurotrophin family of secreted proteins, which include in addition to BDNF, nerve growth factor (NGF) and neurotrophin 3-6 (NT-3-6). BDNF mediates its functions by activating two cell surface receptors, pan-neurotrophin receptor (P75NTR) and tropomyosin-related kinase B (TrkB) and their downstream intracellular cascades. BDNF is best known for its role in neuronal survival, regulation of neuronal differentiation, migration and activity-dependent synaptic plasticity. However, BDNF is widely expressed in non-neuronal tissues as well. The localization and the function of BDNF in intestinal smooth muscle cells (SMCs) are not well defined. Thus, the main purpose of …

Racial/Ethnic Differences In Fatality Rates From Motor Vehicle Crashes: An Analysis From A Behavioral And Cultural Perspective, Huda Hamdan

Theses and Dissertations

Ethnic/racial minorities in the United States are overrepresented in fatalities from motor vehicle crashes (MVC). Growing evidence indicates that there are differences among racial/ethnic groups in risk of involvement in fatal crashes. Based on previous research, numerous factors may be involved in high racial/ethnic fatality rates from MVCs, including failure to use safety equipment, driving while under the influence of alcohol/drug, red light running, and speeding. Using data from the Office of the Chief Medical Examiner (OCME) and the FR300P Police Crash Report, this project explores differences in variables associated with traffic safety behavior and traffic law obedience between non-White …

Sildenafil Attenuates Ethanol-Induced Cardiomyocyte Injury And Preserves Cardiac Function Through Protein Kinase G-Dependent Signaling, Gregory R. Sturz

Theses and Dissertations

Background: Ethanol is a cardiotoxic substance that damages the heart by increasing apoptosis, free radical formation and calcium overloading. Consequently, there is an increase in cell death leaving fewer functioning myocytes leading to heart failure. Sildenafil is a phosphodiesterase type-5 (PDE-5) inhibitor approved for treatment of erectile dysfunction. Studies from our lab have demonstrated that PDE-5 inhibition reduces myocardial infarct size and attenuates post-ischemic cardiac dysfunction in both ischemia-reperfusion and permanent coronary artery ligation models. Therefore, in the present study, we hypothesized that treatment with sildenafil will prevent cardiotoxicity associated with acute alcohol exposure by reducing myocyte apoptosis and preserving …

Regulation Of Gastrointestinal Smooth Muscle Function By Hydrogen Sulfide, Ancy Nalli

Theses and Dissertations

Inhibitory neurotransmitters, chiefly nitric oxide and vasoactive intestinal peptide, cause MLC20 dephosphorylation and muscle relaxation via inhibition of myosin light chain (MLC) kinase and activation of MLC phosphatase. Hydrogen sulfide (H2S) produced as a byproduct by luminal sulfate-reducing commensal bacteria or as an endogenous signaling molecule synthesized from L-cysteine via cystathionine-γ-lyase (CSE) and cystathionine-β-synthase (CBS) regulates muscle contraction. However, the role of H2S in the regulation of MLC phosphatase activity and MLC20 phosphorylation is not known. The aim of the present study was to examine the expression of CSE and CBS in smooth muscle cells and to elucidate the molecular …

Studies On The Reaction Of High-Dose Hydroxocobalamin And Ascorbic Acid With Carbon Monoxide: Implications For Treatment Of Carbon Monoxide Poisoning, Joseph Roderique

Theses and Dissertations

Based upon experimental evidence from the 1970’s we proposed that a reduced form of hydroxocobalamin should be capable of producing carbon dioxide (CO2) from carbon monoxide (CO) in blood, and that this conversion should be detectable. Using resonance raman spectroscopy we demonstrated that a mixture of hydroxocobalamin and ascorbic acid could create the reduced form of hydroxocobalamin. We used a closed-loop circulation system with a hollow-fiber membrane oxygenator to produce carboxyhemoglobin. Using sensitive gas monitoring equipment to the gas-out port of the oxygenator we analyzed the CO and CO2 concentrations coming from the oxygenator. The mixture of hydroxocobalamin and ascorbic …

Management Of Anticoagulation In Patients Undergoing Catheter Ablation Of Atrial Fibrillation, Traci L. Buescher

Theses and Dissertations

No abstract provided.

Diet Options For Cardiovascular Risk Reduction, Ruth Eckstrom

Theses and Dissertations

Cardiovascular disease is a leading killer in the United States and worldwide. Diet as a modifiable risk factor can have a beneficial impact on the reduction of cardiovascular risk. The purpose of this document is to determine the evidence that supports the most effective cardioprotective dietary interventions which can be recommended to patients to reduce the cardiovascular risk factors of elevated low density lipoprotein cholesterol (LDL-C) and hypertension. An extensive review of the literature was conducted to detinning the evidence that supports the most effective cardio-protective dietary interventions using Pub Med, CINAHL, and the World Wide Web. Based on the …

The Many Faces Of Lyme Disease, Blythe H. Kersula

Theses and Dissertations

Lyme disease is the most common vector-borne disease in the United States today, and cases

continue to grow every year. Lyme disease can be challenging to diagnose due to the many

presenting symptoms. This independent study is meant to describe symptoms of Lyme Jess

traditionally discussed in medical and nursing literature when considering how and when to

~ diagnose Lyme disease. It is also meant to examine common diagnostic problems in pediatric,

maternal, and elderly populations. This was accomplished with the use of a literature review, ~ resulting in the development of a white paper and educational brochure for dissemination …

Mask, Mannequin, And The Modern Woman: Surrealism And The Fashion Photographs Of George Hoyningen-Huene, Hillary Anne Carman

Theses and Dissertations

In this thesis I consider photographs of the mannequin by Vogue's fashion photographer, George Hoyningen-Huene. Little scholarship has been written on Huene, as well as many other fashion photographers of the twentieth century. I examine four of Huene's works and his appropriation of the surrealist aesthetic, specifically the use of the mask and mannequin, which were directed at female spectators during the interwar atmosphere and development of the identity of the interwar modern woman. These images include Life-mask of Dolores Wilkinson (1933), Antoine with One of His Creations (1933), Scarf and Gloves by Chanel, Mannequin by Pierre Imans (1934) …

The Role Of Nicotinic Acetylcholine Receptors In Ethanol Responsive Behaviors And Drinking, Anton Dawson

Theses and Dissertations

The high co-morbidity between alcohol (ethanol) and nicotine abuse suggests that nicotinic acetylcholine receptors (nAChRs), which are thought to underlie nicotine dependence, may also be involved in alcohol dependence. A genomic region that encodes the Alpha5* nAChR subtype has recently been shown to be associated with alcohol dependence phenotypes in humans. Therefore, the aim of this study was to determine the role of Alpha5* nAChRs in ethanol-responsive behaviors upon acute administration in mice as well as in their drinking behavior. We conducted tests in mice lacking the Alpha5 coding gene (Chrna5) in ethanol-induced hypothermia, hypnosis, anxiolysis, and conditioned place preference. …

The Influence Of Media On Himba Conceptions Of Dress, Ancestral And Cattle Worship, And The Implications For Culture Change, Austin Sterling Cameron

Theses and Dissertations

For the Himba, deeply embedded cultural symbols—traditional daily way of life, traditional beliefs about the sacredness of cattle, and religious beliefs of ancestral worship—are of ancient origin and have been retained in their culture throughout all of recorded history. While they still exist in Himba society today, some scholars have observed a potential widespread generational shift in adherence to these core cultural values and beliefs. This study presents the findings of 41 in-depth interviews with members of the Himba tribe in northern Namibia—specifically Opuwo and Otutati—ages 18 to 65. It examines the degree to which cultural differences are emerging as …

Evaluation Of The Thorax Of Manduca Sexta For Flapping Wing Micro Air Vehicle Applications, Brian C. Cranston

Theses and Dissertations

The tobacco hornworm hawkmoth (Manduca sexta) provides an excellent model from which to gather knowledge pertaining to the development of a Flapping Wing Micro Air Vehicle (FWMAV). One of the major challenges in design of a FWMAV is the energy demanding nature of low Reynolds number flapping flight. Therefore, an understanding of the power required by the flight muscles to actuate the wings is essential for the design of a FWMAV. The M.sexta wing/thorax mechanism was evaluated as a mechanical system in order to gain insight to the mechanical power required to produce the full natural wing stroke. A unique …

Molecular Analysis Of The Localization And Function Of Caspase-Like Proteins In Dictyostelium Discoideum And The Parasitic Protozoan Acanthamoeba Castellanii, Entsar Jabbar Saheb Kano

Theses and Dissertations

Two novel families of caspase-like proteins, metacaspases and paracaspases, have been discovered but have remained poorly characterized. Metacaspase type-1 (Acmcp) has been identified in Acanthamoeba castellanii , which is an opportunistic protozoan parasite that causes several diseases in humans. On the other hand, paracaspase (Ddpcp) has been found in the protozoan Dictyostelium discoideum . D. discoideum is well-researched model organism that is appropriate for studying these caspase-like proteins. In this study, we investigated the localization and molecular function of metacaspase and paracaspase proteins by over-expressing them in D. discoideum . Separate cell lines over-expressing a GFP-tagged version of the full …

Glycoconjugates Prevent Bacterial Dissemination And Cellular Death During B. Cereus/ B. Anthracis Infection, Mohamed Hassen Lahiani

Theses and Dissertations

Bacillus cereus group species including Bacillus cereus and Bacillus anthracis produce highly resistant spores. Dormant spores can survive for long period in harsh environmental conditions. Spores are engulfed by macrophages. Mammalian macrophages are the first line defenders immune cells through its phagocytic activity. Glycoconjugates (GCs) are a family of synthetic carbohydrates that possesses numerous bactericidal and immunodulatory properties. In my study, I used a multivariate approach to study the effects of GCs on B. cereus and B. anthracis Sterne phagocytosis. During in vitro study, I found that GC1 and GC3 were the best candidates that inhibited B. cereus and B. …

Relationship Between The Quality Of Educational Facilities, School Climate, And School Safety Of High School Tenth Graders In The United States, Darnell Bell

Theses and Dissertations

The purpose of the study was to understand the relationships among facility conditions, school climate, and school safety of high school tenth graders in the United States. Previous research on the quality of educational facilities influence on student achievement has varied. Recent research has suggested that the quality of educational facilities may have an influence on student achievement. If a relationship exists between the quality of educational facilities and student achievement, information and insight can be provided to school districts, legislatures and school leaders for creating budgets for facilities plans. Administrators and Students Questionnaires, and the Facilities Checklist of the …

Meaningful Learning Experiences: The Influence Of Group-Based Adventure Recreation On Behavioral Addiction Treatment, Robert Levon Henderson

Theses and Dissertations

Adventure recreation (AR) has been found to supplement addiction treatment and recovery in valuable ways. However, questions about the components and processes of AR and its influence on behavioral addiction specifically, e.g., gambling, video gaming, pornography, etc., still remain. Using the Meaningful Learning Experiences Model (an AR-based framework) and a quasi-experimental pre-post design, the present study examined AR's influence on three therapeutic principles (authenticity, social network support, and therapeutic engagement) that have been found to significantly influence addiction recovery. Seventy-five participants with sexually related addictions, namely pornography addicts, supplemented their 12-Steps recovery meetings with a Meaningful Learning Experience and scuba …

Integrating New Literacies, Namely Visuals And Video, Into A Traditional, Basal-Text Driven Curriculum In A Secondary Classroom, Stephanie Moon

Theses and Dissertations

The purpose of this thesis was to create a curriculum for a secondary language arts classroom that took traditional lesson plans from an anthology and integrated visuals/videos into the lesson plans to increase rigor, relevance, and engagement in the classroom. Each unit starts with visuals/video then moves to texts and the anthology. This progression is designed to allow the students to move from a familiar place where they feel comfortable and confident, the visual/video, to a medium they are less comfortable and confident with, the text. Another goal of this curriculum is overwhelming engagement throughout each unit. Also, by building …

Part I: An Artificial Neural Network Model Of Tuberculosis Patient Data, Part Ii: A Dft Computational Model Of Metal Hydrides, William Orson Griffin

Theses and Dissertations

PART I -- The data of tuberculosis patients and a control group were analyzed with the artificial neural network in order to find genetic factors which inhibit and promote the patient to developing drug resistant tuberculosis. The data came from populations in Kyrgyzstan containing genetic and physical records as well as the type of tuberculosis they had contracted. Three models based on the data were developed: first, a model which differentiates TB from control patients, second, a model which differentiates both TB and control patients, and finally, a model which distinguishes infiltrative from chronic type tuberculosis. Factors which influenced the …

Machine Conscious Architecture For State Exploitation And Decision Making, Mark M. Derriso

Theses and Dissertations

This research addressed a critical limitation in the area of computational intelligence by developing a general purpose architecture for information processing and decision making. Traditional computational intelligence methods are best suited for well-defined problems with extensive, long-term knowledge of the environmental and operational conditions the system will encounter during operation. These traditional approaches typically generate quick answers (i.e., reflexive responses) using pattern recognition methods. Most pattern recognition techniques are static processes which consist of a predefined series of computations. For these pattern recognition approaches to be effective, training data is required from all anticipated environments and operating conditions. The proposed …

Emulation Of Industrial Control Field Device Protocols, Robert M. Jaromin

Theses and Dissertations

It has been shown that thousands of industrial control devices are exposed to the Internet, however, the extent and nature of attacks on such devices remains unknown. The first step to understanding security problems that face modern supervisory control and data acquisition (SCADA) and industrial controls networks is to understand the various attacks launched on Internet-connected field devices. This thesis describes the design and implementation of an industrial control emulator on a Gumstix single-board computer as a solution. This emulator acts as a decoy field device, or honeypot, intended to be probed and attacked via an Internet connection. Evaluation techniques …

Investigation Into The Ratio Of System Operations And Support Costs To Life-Cycle Costs For Department Of Defense Weapon Systems, Gary L. Jones

Theses and Dissertations

As the arsenal of weapons managed by the Department of Defense (DoD) ages, the country's leadership is forced to make decisions regarding what to do with current and projected funding to maintain military defensive and offensive capabilities. The acquisition community at many levels has stated that O&S costs are about 70 percent of the total life-cycle costs of the average system, the other 30 percent being spent in the acquisition phase. This golden ratio does not appear to come from empirical evidence. There has been little research into the actual ratio of O&S-to-acquisition costs. This research found a significant departure …

Aerial Refueling Simulator Validation Using Operational Experimentation And Response Surface Methods With Time Series Responses, Alexander P. Hillman

Theses and Dissertations

An important program in the Department of Defense is the KC-46 Supertanker. Dubbed the future of the Air Force's aerial refueling inventory, the KC-46 will replace dozens of ailing previous generation tanker aircraft. The Aerial Refueling Airplane Simulator Qualification document governs the methods by which Air Mobility Command validates its simulators, some of which will be KC-46 simulators in the near future. The methodology set forward in this thesis utilizes historical data of aircraft performance from similar air frames to gain statistical insight into the performance design space of the KC-46. Leveraging this insight, the methodology provides through a framework …

Analysis Of Effects Of Organizational Behavior On Evolving System Of Systems Acquisition Programs Through Agent Based Modeling, Klayton S. Bobsein

Theses and Dissertations

Every weapon system operates in context with one or more system of systems (SoS). It is the SoS that provides warfighting capability. Each system is managed independently by a program office with program-centric priorities. These systems must be interconnected and interoperable, so the program office must collaborate across the SoS. The SoS are always changing and evolving, triggered by variety of stimuli. Acquisition program offices can be characterized with a set of organizational behaviors that respond to the environment, are influenced by SoS architecture, and can be described by their fitness and contribution to SoS. Using Geert Hofstede's cultural dimensions, …

Fate Of Malathion In An Activated Sludge Municipal Wastewater Treatment System, Edward B. Walters

Theses and Dissertations

Organophosphate compounds are used as pesticides and in chemical warfare agents such as nerve agents. VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioate) is one of the most toxic and environmentally persistent of these nerve agents. This research examined the fate of malathion, a pesticide and surrogate of VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioate), in bench-scale activated sludge (AS) sequencing batch reactors. Sorption kinetics and sorption equilibrium isotherm experiments indicate that sorption to AS biomass was not a statistically important removal mechanism. However, approximately 90% of the initial 4.25 mg L-1 malathion concentration degraded primarily via heterotrophic activity.