Digital Commons Network^™

Identification Of Virulence Factors In Edwardsiella Ictaluri, Jingjun Lu

Theses and Dissertations

Edwardsiella ictaluri is the causative agent of enteric septicemia of catfish (ESC), which is one of the most important diseases impacting the US catfish industry. Though this disease has been very common, progress has been slow to find an economical and practical treatment method. Our long-term goal is to determine the mechanisms of E. ictaluri virulence in ESC. The overall objective of this study was to identify E. ictaluri genes required for host encounter and serum resistance and to determine their roles in pathogenesis. The central hypothesis is that E. ictaluri must differentially regulate its genes to invade fish and …

An Exploration Of Carbon-Filled Carbon Nanotubes As A Potential Material In Coronary Stents, Kristopher Neil Jones

Theses and Dissertations

The purpose of this research is to explore the potential of using carbon-infiltrated carbon nanotubes (CI-CNT) as a material for coronary artery stents. Stents are commonly fabricated from metal, which may not perform as well as many polymers and ceramics in biomedical applications. Pyrolytic carbon, a ceramic, is currently used in medical implant devices due to its preferrable biocompatibility properties. Micro-patterned pyrolytic carbon devices can be created by growing carbon nanotubes, and then filling the space between with amorphous carbon via chemical vapor deposition. We prepared multiple samples of two different planar stent-like flexible geometries and smaller cubic structures out …

Domestic In Nature, Emily Shepardson

Theses and Dissertations

I am a painter and a printmaker. My imagery consists of houses, barns, birds, trees, hands, and gloves. On the surface these items represent home, nature, and female identity. On another level they symbolize an inner world of dreams, wishes, and losses. My paintings contain aspects of collage, they combine paint, paper, and low relief. I paint layers of transparent and opaque images and colors in order to achieve a dreamy and ethereal effect. In printmaking, I combine my imagery in layers by printing small plates and stencils next to and on top of one another until a dense, multifaceted …

Work | Space (The Laborer Revealed), Nicole Farrand

Theses and Dissertations

There is always a moment, within the act of work, when the decision comes into question; the mind is aware of the goal, seeking out the destination, but the body brings the reality of the task to light, at first whispering its doubts, then speaking in full volume. The physical intensity of the creation of my work enlivens this debate between the consciousness of my intellect and the limitations of my being. The act of defining myself as an artist serves as a challenge to my body’s capabilities as a maker. Through this relationship with my work, I have become …

Modernization Of The Mock Circulatory Loop: Advanced Physical Modeling, High Performance Hardware, And Incorporation Of Anatomical Models, Charles Taylor

Theses and Dissertations

A systemic mock circulatory loop plays a pivotal role as the in vitro assessment tool for left heart medical devices. The standard design employed by many research groups dates to the early 1970's, and lacks the acuity needed for the advanced device designs currently being explored. The necessity to update the architecture of this in vitro tool has become apparent as the historical design fails to deliver the performance needed to simulate conditions and events that have been clinically identified as challenges for future device designs. In order to appropriately deliver the testing solution needed, a comprehensive evaluation of the …

Automatic Segmentation Of Pressure Images Acquired In A Clinical Setting, Anathea Pepperl

Theses and Dissertations

One of the major obstacles to pressure ulcer research is the difficulty in accurately measuring mechanical loading of specific anatomical sites. A human motion analysis system capable of automatically segmenting a patient's body into high-risk areas can greatly improve the ability of researchers and clinicians to understand how pressure ulcers develop in a hospital environment. This project has developed automated computational methods and algorithms to analyze pressure images acquired in a hospital setting. The algorithm achieved 99% overall accuracy for the classification of pressure images into three pose classes (left lateral, supine, and right lateral). An applied kinematic model estimated …

Inhibition Of Retinoic Acid Receptors Results In Defasciculation Of The Trigeminal Nerve In Xenopus Laevis, Jeremy Thompson

Theses and Dissertations

The anatomy of the cranial peripheral nervous system has been studied for over a century, yet surprisingly little is known about how the nerves are guided to their targets. The study of the development of these nerves has important implications for our understanding of craniofacial anomalies and possible treatments for both injury and genetic disorders of nerve development such as Goldenhar-Gorlin syndrome. We have discovered that retinoic acid (RA) may play a role in the development of the trigeminal nerve. Inhibition of retinoic acid receptors (RAR) results in trigeminal nerves that become unbundled or defasciculated in the eye region. To …

Fragments Of Social Life, Rachel Leah Cohn

Theses and Dissertations

This paper examines selected events from biography and how those events have influenced my philosophies about art-making as well as the work I have produced while a graduate student at Virginia Commonwealth University. This thesis is an attempt to give an expanded context for my work through various lenses, including the personal, the traumatic, the historical and the material.

Remembering To Look Down: A Thing Not A Thesis, George Ian Mcmahon

Theses and Dissertations

Thoughts on magic, spectacle, arena, material, materiality, marrow, invincibility, repetition, performance, sculpture, time, decay, temporality, tools, marks, gestures, writing, coaxing, site, stage, architecture, and movement.

Effects Of Ischemia And Reperfusion On The Local Regulation Of Oxygen Consumption, Tissue Oxygenation And Blood Supply In Rat Skeletal Muscle., Sami Dodhy

Theses and Dissertations

In resting muscle, blood flow is regulated to meet the demand for O2 by the tissue. A modified ischemia (I)/reperfusion(R) investigation was systematically run and PISFO2, PaO2, Q and VO2 were observed. Twenty-nine spinotrapezius muscles from male Sprague-Dawley rats (284±20 grams) were surgically exteriorized for intravital microscopy to test a model relating blood flow, O2 supply and O2 demand. The model can aid in the understanding of the regulation of tissue PO2. The interstitial PO2 (PISFO2) and perivascular PO2 (PaO2) measurements were made using phosphorescence quenching microscopy (PQM). O2 consumption (VO2) values were obtained with a quasi-continuous, flash-synchronized, pressurized airbag …

The Effects Of Intervention Involving A Robot On Compliance Of Four Children With Autism To Requests Produced By Their Mothers, Holly Jeanne Nelson

Theses and Dissertations

The current study presents the use of a humanoid robot to facilitate compliant behaviors to two types of directives in four children with autism. The children participated in a three-month intervention program that incorporated core components of the SCERTS model in order to facilitate social communication (Prizant, Wetherby, Rubin, & Laurent, 2003). Treatment sessions were comprised of 40 minutes of traditional treatment and 10 minutes of interaction with a humanoid robot. Pre- and post-intervention assessment were conducted, each involving a 5 minute interaction with the child's mother, in which they were presented with directives in the form of physical manipulation …

Shift, Gittins George

Theses and Dissertations

Using Eduard Manet and Alex Katz as an entry point into my work, I will discuss seven of my paintings in detail. I will examine formal structures within the work such as color and space and how they relate to the content. I will discuss ideas of voyeurism and notions of the gaze while examining film as a presence. Artifice, art history, desire, personal memory, nostalgia and everyday life are starting points for formal explorations within the work.

Hissār, Sohail Abdullah

Theses and Dissertations

Hissaar is a noun and a verb, it is the periphery and the extremities, and the walls and the fortress. And it is to encircle, to wrap and to contain. This paper is an inexhaustive account of thoughts, experiences and lessons learned, of varying forms that influence my aesthetic sensibilities, my art-value system, and my art- ethical concerns. They provide for my art the impetus for its perpetual (and perhaps circular) journey. It is about finding connections between the fraying ends of free floating ideas. The following fragments explores how words make ideas, ideas make images, images make memory; memory …

Naturalis Historia, Reconstructed, Sarah Briland

Theses and Dissertations

When Pliny the Elder wrote Naturalis Historia around 70 A.D., the idea of natural history contained and connected biology, geology, and mineralogy with the history of painting and sculpture. Art was an extension of the natural world as its materials were extracted from plants, animals, and, particularly, mined and quarried pigments, stone, and metals. In my developing body of work, Incidents of Naturalis Historia, Reconstructed, I combine wasps’ nests, architectural fragments, and other found objects excavated from my surrounding environment with elements of glass that resemble lichen, crystallization, and geologic specimens. These works simulate artifacts of an alternative history; one …

Locus, Claire Krueger

Theses and Dissertations

This thesis explores my practice as it has progressed into video and video installation. I detail my use of cinematic tropes and mechanisms as they function within a spatial installation. I discuss the relationship of my work to other artists such as Pierre Huyghe, Janet Cardiff and George Bures Miller, and Kevin Cooley who also deal with themes of landscape, spatial displacement, and video viewing. My work has evolved to video installation from a need to experience the traditionally flat viewing plane of photography in a more experiential way. The Locus installation is multi sensory, in that it addresses smell, …

Differential Behavioral Effects Of Ketamine Between Adolescent And Adult Sprague-Dawley Rats, Maria A. Greenwood

Theses and Dissertations

The dissociative anesthetic ketamine has been subject to growing abuse worldwide, particularly in adolescents. This project compared the effects of ketamine in conditioned place preference and intravenous self-administration in adolescent (PND 28-50) and adult (>PND70) Sprague-Dawley rats. Cocaine served as a positive control. In CPP, adolescents demonstrated preferences for ketamine, while adults developed an aversion. In the self-administration procedure, adults acquired the behavior more rapidly, but there was no difference in the percentage of subjects reaching acquisition nor in responding under a progressive ratio schedule for either drug. The CPP results suggest that adolescents have a greater sensitivity to …

Underlying Conditions, Mariam Eqbal

Theses and Dissertations

The following is a collection of lines, curves, and dots on a plane, representing words fashioned to communicate my ideas about relationships between the thing and the nothing. This thesis is like a map, a contour drawing, an assortment of lines, speaking my thoughts about things in relation to space and time. As something moving, developing into another thing, as a thing sentient, I am viewing, performing, and processing incremental acts. I am looking at water and I can see my reflection break into a thousand pieces. It is like watching time.

Narrowing The Gap Between Imaginary And Real Artifacts: A Process For Making And Filming Diegetic Prototypes, Al Hussein Wanas

Theses and Dissertations

Critical Design uses designed artifacts as a critique of consumer culture. However, the complex nature of these artifacts prompted designers to focus on the artifact and present it in an informative, but relatively isolated fashion.The theoretical framework for this thesis is drawn from a similar, yet more recent, design criterion called Design Fiction. The artifacts of Design Fiction are called Diegetic Prototypes: fictional prototypes that function in the social sphere of a film’s structure. This research develops a method for analyzing and creating artifacts, in reference to psychoanalysis theories on the human psyche and perception of objects. It then explores …

The Quest For A Transcendental Hero, William Joseph Nolen

Theses and Dissertations

This work begins by briefly exploring the historical, cultural, and economic factors springing up from the rise of scientific and economic materialism in the crux of capitalism. It is argued that the bourgeois value system strangled the possibility of heroic action in the public arena and thereby eliminated the poet’s ability to find a heroic figure in nineteenth century America. The focus is then shifted to the Transcendentalist movement (Emerson, Thoreau and Fuller), who fear the loss of the hero as the loss of inspiration for mankind and the loss of subject matter for poets. The works and ideas of …

Tissue Engineering Composite Biomimetic Gelatin Sponges For Bone Regeneration, Isaac Rodriguez

Theses and Dissertations

The field of tissue engineering aims to develop viable substitutes with the ability to repair and regenerate the functions of damaged tissue. Common practices to supplement bone regeneration in larger defects include bone graft biomaterials such as autografts, allografts, xenografts, and synthetic biomaterials. Autologous bone grafting is the current gold-standard procedure used to replace missing or damaged bone. However, these grafts have disadvantages such as donor site morbidity, limited availability, and the need for a secondary surgery. The focus of this study is to tissue engineer a lyophilized gelatin composite sponge composed of hydroxyapatite (HA), chitin whiskers (CW), and preparations …

Shockheaded Peter: A Junk Opera. Costume Design., J. Theresa Bush

Theses and Dissertations

My concept is rooted in the fairy tail land of the dark woods like so many other terrifying fairy tails from my youth. Shockheaded Peter, our boogie man, is of the trees. The blood of the rotten children feeds the trees in this concept for the marbles that fall from our children literally representing blood will flow down the rake, across tin gutters, and into a huge tin washbasin out of which grows our gnarly set tree, a gnarly knobby willow. Peter comes from this tree that is fed by the blood of children. I set my costumes in the …

Determinants Of Abuse-Related Effects Of Monoamine Releasers In Rats, Clayton T. Bauer

Theses and Dissertations

Monoamine releasers constitute a class of compounds that promote release of dopamine, serotonin, and/or norepinephrine. These compounds have a range of different uses in the medical setting, including treatment of attention deficit hyperactivity disorder, narcolepsy, and obesity. A major limitation of many of these compounds (i.e. amphetamine, methamphetamine, phenmetrazine) is their propensity for abuse; however, not all monoamine releasers are abused (i.e. fenfluramine). The goal of this dissertation was to examine pharmacological determinants of abuse-related effects produced by monoamine releasers in two preclinical assays in rats: intracranial self-stimulation (ICSS) and drug discrimination. First, this work confirmed and expanded upon previous …

Accuracy Of Limited Field Cone Beam Computed Tomography In The Detection Of Buccal Cortical Plate Perforations Due To Periapical Lesions, Dan-Linh Ha

Theses and Dissertations

Pre-surgical planning for endodontic microsurgery is facilitated by the use of cone beam computed tomography (CBCT). The purpose of this study was to determine whether limited field CBCT accurately predicts buccal cortical plate perforations due to endodontic lesions. Thirty-five roots that underwent microsurgical root end resection were included in this study. Prior to the surgery, 90 voxel CBCTs were taken with a Carestream 9300. The scans were analyzed by an endodontic resident and oral radiologist to determine the presence of a perforation in the buccal plate. These findings were compared to the clinical appearance of the bone. There was a …

Lashes To Ashes, Exploring The Hidden Dimensions Of Human Hair, Rania Chamsine

Theses and Dissertations

Hair is power, beauty and seduction: a reflection of ethnicity and religion, and even a canvas for self-expression. A key feature in defining identity and social status, it holds the essence of our individuality. However, once removed from its original and natural setting—the epidermis—hair is seen as waste, and often evokes disgust. The objective of this thesis is to explore human hair, which particularly in the Arabic-Islamic region, carries great significance and raises many religious, cultural, and gender issues. Through design, and informed by critical design theory, I explore how this corporeal material can be reused and re-presented as a …

Not Another Space Suit: Fusing Technology And Indigenous Solutions To Facilitate Thermal Comfort, Alia Khairat

Theses and Dissertations

Unseen, unheard and unconsidered, Qatar’s migrant worker population is building one of the richest countries in the world. They labor in Qatar’s high heat index1 climate, which is coincidentally comparable to an Oriental sauna, wearing the most rudimentary of clothes. Working up to 60 hours a week, migrant workers fall victim to heat stress and dozens are hospitalized daily, starting as early as March and increasing in numbers during the peak months of June to August. Since clothes are essentially a “second skin,” affecting the rate and efficiency with which heat is exchanged between the body and its surroundings, a …

Microbial Evaluation Of The Calamus Heated Gutta-Percha Delivery System, Christopher Smart Dds

Theses and Dissertations

If gutta-percha cartridges are contaminated with bacteria prior to obturation then contamination of the root canal system may result. Successful treatment not only depends on bacterial elimination but also depends on prevention of recontamination. This study was motivated by the observation that endodontists frequently use single use gutta-percha cartridges on multiple patients. The goal of this study was to determine if cross contamination occurred when a single gutta-percha cartridge in the Calamus™ Flow System was used on multiple patients. An effective recovery method was established during a pilot study using Enterococcus faecalis as a sample bacterium. Microbial recovery was obtained …

Effects Of Mu Opioid Receptor Agonists On Intracranial Self-Stimulation In The Absence And Presence Of “Pain” In Rats, Ahmad Altarifi

Theses and Dissertations

Pain is a significant health problem. Mu opioid receptor agonists are used clinically as analgesics, but their use is constrained by high abuse liability. Intracranial self-stimulation (ICSS) is a preclinical behavioral procedure that has been used to assess abuse potential of opioids, and drug-induced facilitation of ICSS is interpreted as an abuse-related effect. ICSS can also be used as a behavioral baseline to detect affective dimensions of pain. Specifically, pain-related depression of ICSS can model pain-related depression of behavior and mood, and drug-induced blockade of pain-related ICSS depression can serve as a measure of affective analgesia. This dissertation used mu …

Pet Therapy And The Associated Benefits On The Elderly, Lindsey Senger

Theses and Dissertations

Pet therapy (also known as Animal Assisted Therapy {AAT}) today involves both human and animal interactions. Pet therapy is utilized most commonly with the elderly living in Long Term Care (LTC) facilities or assisted living facilities to promote health and healing for the patients. It is especially beneficial for those who are chronically ill, may suffer from disabilities or may have depression. The feeling of isolation and loneliness that accompanies depression is not healthy, especially for the elderly population. Research has shown that the health and well-being of the elderly who suffer from depression, decline rapidly when compared to those …

Evaluation And Management Of The Adult Patient With Dermatomyositis: A Review For The Advanced Practice Nurse, Tana L. Bock

Theses and Dissertations

PURPOSE: To examine a case of adult dermatomyositis (DM) and provide a clinical review that clarifies the pathogenesis, diagnosis, evaluation, and management of DM in the adult patient.

DATA SOURCES: Findings from the history, physical examination, and diagnostic testing of a 57-year-old woman with dermatomyositis. A search of literature from 1970 to 2013 was conducted including textbooks, Pub Med and CINAHL databases with the search terms "dermatomyositis," "idiopathic inflammatory myopathies," "polymyositis," "pathogenesis," "myopathies," "pathogenesis," "therapy," and "treatment."

CONCLUSION: Dermatomyositis is one of the idiopathic inflammatory myopathies affecting individuals of all ages. Its underlying pathology is related to …

Race, Power, And White Womanhood: The Obsessions Of Tom Watson And Thomas Dixon Jr., Tara Nicole Kowasic

Theses and Dissertations

Thomas Dixon Jr. (1864 -1946) and Thomas E. Watson (1856-1922), two controversial and radical figures, are often credited with the second coming of the Ku Klux Klan. Dixon, writer of novels and plays such as The Leopard’s Spots (1902) and The Clansman (1905), and Watson, politician, prolific writer, and publisher of Watson’s Magazine and The Jeffersonian, reached the masses and saturated popular culture with their racial agenda. As each of these men had especially long careers, this thesis focuses on particular times and specific issues. With Dixon, the writing of The Clansman (1905) and production of The Birth of a …