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Tung Tried: Agricultural Policy And The Fate Of A Gulf South Oilseed Industry, 1902-1969, Whitney Adrienne Snow May 2013

Tung Tried: Agricultural Policy And The Fate Of A Gulf South Oilseed Industry, 1902-1969, Whitney Adrienne Snow

Theses and Dissertations

The U.S. tung oil industry began as a government experiment in plant diversification but businessmen mistakenly interpreted this interest as an endorsement of domestic production and began growing tung trees in the Gulf South states of Florida, Georgia, Alabama, Mississippi, Louisiana, and Texas. The new crop quickly caught the attention of paint, varnish, and ink companies in the northern and Midwestern states and created a buzz among chemurgists like Henry Ford and other industrialists who eagerly expanded tung acreage. With the erection of the first crushing mill in 1928, the tung oil industry began but it did not acquire any …


Comparison Of Indigenous And Bio-Augmented Pentachlorophenol (Pcp) Degrading Bacteria For Remediation Of Pcp In Contaminated Groundwater, Vaibhav V. Joshi May 2013

Comparison Of Indigenous And Bio-Augmented Pentachlorophenol (Pcp) Degrading Bacteria For Remediation Of Pcp In Contaminated Groundwater, Vaibhav V. Joshi

Theses and Dissertations

The objective was to compare pentachlorophenol (PCP) degradation in contaminated groundwater by indigenous and bio-augmented (Sphingobium chlorophenolicum and Burkholderia cepacia) PCP degrading bacteria. Indigenous bacteria were identified by cloning and sequencing of 16S rDNA fragments while PCP concentrations were determined by GC-ECD. Gene expression for PCP degrading enzymes: chlorophenol 4-monooxygenase (TftD, B. cepacia) and pentachlorophenol-4-monooxygenase (pcpB, S. chlorophenolicum), was determined by RT-PCR. B. cepacia, a PCP degrading bacteria was identified as dominant indigenous bacteria. PCP concentrations correlated negatively with PCP tolerant bacteria and relative fold gene expression in treatments with air-sparging (phase2) compared to without air-sparging (phase1). PCP concentrations decreased …


Conversation Of Values: A Community Perspective Of Sustainable Development Criteria Concerning Revitalization Efforts In Jackson, Mississippi, Kenneth Barry White May 2013

Conversation Of Values: A Community Perspective Of Sustainable Development Criteria Concerning Revitalization Efforts In Jackson, Mississippi, Kenneth Barry White

Theses and Dissertations

Jackson, Mississippi is currently undergoing a revitalization movement in an attempt to revive its blighted downtown core. While physical development is crucial to revival of the downtown landscape, the cultural landscape must also be considered. I hypothesized that developers, business owners, and residents working or living in and around downtown Jackson would report differing desires, positions, and values concerning four elements of sustainable development: cultural, economic, political, and environmental. Ultimately, the hypothesis was refuted according to the quantitative data analysis. However, there were different understandings within the qualitative data where the substance of this research project can be found. These …


Creation Of A Computational Simulation Of Maternal Trauma In Motor Vehicle Accident, Benjamin C. Weed May 2013

Creation Of A Computational Simulation Of Maternal Trauma In Motor Vehicle Accident, Benjamin C. Weed

Theses and Dissertations

Maternal trauma is the leading non-obstetric cause of maternal and fetal death. Because the anatomy of a pregnancy is distinct, and highly transient, the pregnant woman and her fetus are both susceptible to injuries which are not seen in the typical trauma patient. The pregnant uterus, the placenta, and the fetus are all relatively poorly supported, as compared with non-transient abdominal or thoracic organs, which can lead to injuries such as uterine rupture, placental abruption, and fetal trauma or death. The leading cause of maternal trauma is automotive collision, and other common causes include violence, falls, and other accidents. Automotive …


Scrotal Castration As A Safe And Effective Means Of Male Canine Sterilization, Kimberly A. Woodruff May 2013

Scrotal Castration As A Safe And Effective Means Of Male Canine Sterilization, Kimberly A. Woodruff

Theses and Dissertations

For years, a prescrotal technique has been the only accepted method of male dog sterilization, as dogs are considered to be “scrotal conscious.” The prevailing thought has been that a scrotal incision will cause more complications including swelling and induction of self-trauma. There is, however, little in the scientific literature that confirms or contradicts this thinking. In this study 437 apparently healthy male dogs over the age of 6 months were randomly allocated into 2 treatment groups and castrated by either a prescrotal (n=206) or scrotal incision (N=231). Complications were recorded up to 72 hours following the procedure. The focus …


Sedimentation Processes Of Perdido Bay, Natalie Jade Sigsby May 2013

Sedimentation Processes Of Perdido Bay, Natalie Jade Sigsby

Theses and Dissertations

The purpose of this research was to identify the forcing factors and processes of sediment transport in Perdido Bay. Data were collected from Perdido Bay in July 2011 and used in the development of a three-dimensional sediment transport model using EFDC as well as in the estimation of a sediment budget. Water and bed samples, water quality readings for salinity, temperature, dissolved oxygen, turbidity, depth, and pH, and velocity measurements were collected. Using field and historical data, an EFDC model was created to simulate the processes of salinity and sediment transport. The model successfully demonstrated the movement of sediment through …


Effect Of Dominance In Captive Female White-Tailed Deer (Odocoileus Virginianus), Eric S. Michel May 2013

Effect Of Dominance In Captive Female White-Tailed Deer (Odocoileus Virginianus), Eric S. Michel

Theses and Dissertations

Factors associated with rank position are poorly understood whereas even fewer studies assessed if benefits were associated with increased rank position when resources were unlimited. I assessed whether age, body mass, size, and testosterone levels were important in rank establishment among 132 captive female white-tailed deer (Odocoileus virginianus). I also assessed if the benefit metrics of improved body condition, decreased stress level, and earlier parturition date were related to rank position. Deer in each of 9 study pens had a linear hierarchy with a mean h Œ of 0.39 (SD = 0.09). Rank position was moderately related to age (P < 0.1) and was strongly related to body mass and size (P < 0.01). There was no relationship between benefit metrics and rank position (P < 0.1). Although increased body mass, size, and age improved rank position there were no benefits associated with increased rank when resources were unlimited.


Effect Of Plant-Based Filtration And Bio-Treatment On Toxicity Of Bio-Oil Process Water, Toktam Moghbeli May 2013

Effect Of Plant-Based Filtration And Bio-Treatment On Toxicity Of Bio-Oil Process Water, Toktam Moghbeli

Theses and Dissertations

This study evaluated physical and biological treatments of bio-oil process water to decrease organic contaminants. A three-sequential-column filtration system compared four treatments: three columns filled with kenaf only; three columns filled with wood shavings only; first column filled with wood shavings and two with kenaf; and first column filled with kenaf and two with wood shavings. The kenaf and wood shavings were composted after filtration. The filtrate water underwent further bio-treatment by adding aeration and selected bacteria. After filtration and bio-treatment, oil and grease concentrations were reduced over 80 percent and toxicity reduced over 90 percent. There were no significant …


Beaver Movements On Managed Land In The Southeastern United States, Lance Forest Mcclintic May 2013

Beaver Movements On Managed Land In The Southeastern United States, Lance Forest Mcclintic

Theses and Dissertations

I studied movement characteristics and vegetative resources effects on home range size of beavers at Redstone Arsenal (RSA) in north central Alabama, USA. Beavers were captured and radio tagged from 11 wetlands during winter and spring of 2011. I monitored movements of radio-tagged beavers using radio telemetry from May 2011–April 2012. Beavers moved faster, presumably more favorable to central place foraging, in wetland as they proceeded farther away from the central place, but did not in upland. Additionally, distributions of hourly distances from lodges were bimodal. Home range, core areas, and distance from lodge did not differ between age classes. …


Characterization, Inheritance, And Marker Identification Of Potential Novel Genes Conditioning Resistance To Multiple Races Of Cercospora Sojina K. Hara Of Soybean (Glycine Max L.), James Brewer Blessitt May 2013

Characterization, Inheritance, And Marker Identification Of Potential Novel Genes Conditioning Resistance To Multiple Races Of Cercospora Sojina K. Hara Of Soybean (Glycine Max L.), James Brewer Blessitt

Theses and Dissertations

Soybean is an economically important crop. It is a selfertilized species grown on vast contiguous acres. These facts predispose soybean to disease epidemics. Cercospora sojina, causal agent of frogeye leaf spot, has reduced United States soybean productivity 0.3 percent on average per year between 2008 and 2010. Several states have reported the pathogen developing resistance to the strobilurin class of fungicides. To date genetic host resistance has been identified as single dominant genes (Rcs1, Rcs2, and Rcs3). However, the lifespans of Rcs1 and Rcs2 were 10 and 16 years respectively. Currently, the Rcs3 locus has been utilized in all major …


A Community That Is Like Family: Conservation Subdivision Residents' Perceptions Of Their Neighborhood, Jamie Elizabeth Lucius May 2013

A Community That Is Like Family: Conservation Subdivision Residents' Perceptions Of Their Neighborhood, Jamie Elizabeth Lucius

Theses and Dissertations

Conservation subdivisions offer an alternative to large-lot residential developments along the urban fringe. These developments pride themselves on protecting ecologically sensitive land and providing an improved quality of life for residents. As suggested by Randall Arendt (1996), these goals are achieved by clustering homes on one portion of the site, while “half or more of the buildable land area is designated as undivided, permanent open space.” In order to gain information about the priorities and motivations of conservation subdivision residents in the Southeast United States, a survey was administered to homeowners within five conservation subdivisions. The results from this study …


Evaluating The Use Of Stable Isotopes, Nirs, And Microbial Populations To Detect Dietary Changes In Dairy Calves, Kathryn Marie Cooley May 2013

Evaluating The Use Of Stable Isotopes, Nirs, And Microbial Populations To Detect Dietary Changes In Dairy Calves, Kathryn Marie Cooley

Theses and Dissertations

Three different methods were developed to evaluate nutritional status: stable isotopes, near infra-red reflectance spectroscopy (NIRS), and microbial populations. In the first method, hair samples were collected, analyzed, stable isotope content greatly differed (P < 0.01) between pre- and post-weaning samples. The second method used NIRS spectral patterns and analyzed fecal samples, and a difference was found around 2100 to 2250 nm where diets containing milk showed a peak, and non-milk diets showed a plateau. Finally, the third method involved profiling microbial populations from fecal samples as calves experienced dietary shifts. Total anaerobes, enterics, and clostridium were different (P<0.05) from pre to post-weaning states. Total anaerobe samples differed (P < 0.005) from milk, milk-grain diets to grain and grain-hay diets. Total aerobes, streptococci, and clostridium samples differed (P <0.001) from the milk diet to the other diets. In summary, all three methods showed differences when comparing pre- to post-weaning states.


Development, Evaluation And Refinement Of A Body Mass Index Formula For Large Breed Canine Patients, Dena Lynn Lodato May 2013

Development, Evaluation And Refinement Of A Body Mass Index Formula For Large Breed Canine Patients, Dena Lynn Lodato

Theses and Dissertations

The current method of quantifying obesity in the veterinary patient is the subjective body condition score; however, scant research has focused on the development of an objective measurement. The present two part study evaluates the ability to create and refine an objective body mass index (BMI) formula for the large breed canine patient. In the first part of this study, seven morphometric measurements were obtained from seventy large breed dogs allowing the creation of five BMI formulas. In the second part of this study, computed tomography images were obtained from twenty-two dogs and the total percent body fat (TBF) was …


Digestibility Of Different Multi-Species Native Warm-Season Grass Mixtures Grown In Varied Harvest Regimen, Janet Moromoke Ogunlade May 2013

Digestibility Of Different Multi-Species Native Warm-Season Grass Mixtures Grown In Varied Harvest Regimen, Janet Moromoke Ogunlade

Theses and Dissertations

Study was conducted to evaluate in vitro digestibility of native warm-season grasses. Three grasses were used: big bluestem (Andropogon gerardii Vitman), little bluestem (Schizachyrium scoparium Nash), and indiangrass (Sorghastrum nutans Nash). There were no differences in NDF, ADF, FAT and OM of the three grass species. However, DM, hemicellulose and CP were slightly different in the three grass species. Also, the frequency nested in cutting effects was determined. In vitro dry matter disappearance of big bluestem, little bluestem and indiangrass was evaluated to determine rate of disappearance. The 100 % indiangrass revealed the greatest rate of disappearance for IVDMD and …


Effect Of Trailer Stocking Density On Cattle Behavior During Truck Transport, Lyndi L'Rae Jury May 2013

Effect Of Trailer Stocking Density On Cattle Behavior During Truck Transport, Lyndi L'Rae Jury

Theses and Dissertations

This experiment was conducted to assess the effects of either low or high trailer stocking density (SD) on cattle behavior. British x Continental steers (n = 200; mean BW = 361.53 ± 54.4 kg) were loaded onto one of two truck-towed trailers. Steer behavior and positional orientation were recorded by video camera (8 h). Aggressive behaviors were more frequent (P < 0.0001) in low (178 ± 34.46 kg BW/m2) than high (333 ± 31.84 kg BW/m2) SD. Chin-resting occurred more often (P < 0.05) in low SD, yet there was no difference (P = 0.98) between SD for mounting. There was a SD × trip effect for loss of balance (P < 0.05), ruminating (P < 0.0001), and lying behaviors (P < 0.0001). At both SD, side-left and side-right were the preferred (P < 0.05) standing orientations. These results suggest that SD can affect cattle behavior and orientation en route.


Use Of Biophotonic Models To Monitor Biological Compounds Via The Angiogenic System, Ramey C. Youngblood May 2013

Use Of Biophotonic Models To Monitor Biological Compounds Via The Angiogenic System, Ramey C. Youngblood

Theses and Dissertations

Angiogenesis is a central process to both physiological and pathological aspects of living organisms. Understanding the angiogenic system via the key mediator, vascular endothelial growth factor (VEGF), has led to the development of biophotonic models capable of monitoring how this process is programmed. The whole animal model tested here is based on the involvement of angiogenesis in a wound healing environment. This model proved to be functional as a system monitor but lacked the precision to yield significant results between the biological compounds tested (estrogen, methoxychlor, and relaxin). The in vitro model is based on angiogenesis in a cancer environment. …


Development Of Safe And Efficacious Live Attenuated Edwardsiella Ictaluri Vaccines Against Enteric Septicemia Of Catfish, Neeti Dahal May 2013

Development Of Safe And Efficacious Live Attenuated Edwardsiella Ictaluri Vaccines Against Enteric Septicemia Of Catfish, Neeti Dahal

Theses and Dissertations

Edwardsiella ictaluri is the causative agent of enteric septicemia of catfish (ESC), which is the most economically important disease of farm-raised channel catfish. E. ictaluri is considered a facultative intracellular pathogen like other well-known species in the Enterobacteriaceae, and it is capable of surviving inside channel catfish neutrophils and macrophages. Its ability to survive inside neutrophils and macrophages has made the development of an effective vaccine against ESC particularly challenging. The goal is to develop a safe, efficacious live attenuated ESC vaccine that is practical and economically beneficial to catfish producers. In this study, single and combination of mutations in …


Fiber Separation From Milled Corn And Sorghum Using The Elusieve Processfor Value Addition To Feed And Biofuel Production, Tejas S. Pandya May 2013

Fiber Separation From Milled Corn And Sorghum Using The Elusieve Processfor Value Addition To Feed And Biofuel Production, Tejas S. Pandya

Theses and Dissertations

Corn is widely used as animal feed as well as for fuel ethanol production. Fiber present in corn is not digested well by non-ruminants such as chicken and swine. Also, this fiber does not participate in conversion of starch to ethanol. Fiber separation from corn flour using the Elusieve process, a combination of sieving and air classification, would result in high starch animal feed and increase ethanol productivity. The objective of the first part of this dissertation was to understand the effect of retention screen size in the hammer mill on fiber separation from corn flour using the Elusieve process. …


Bacterial Community Succession During Soil And Ecosystem Development, Shankar Ganapathi Shanmugam May 2013

Bacterial Community Succession During Soil And Ecosystem Development, Shankar Ganapathi Shanmugam

Theses and Dissertations

Organism succession during ecosystem development has been well studied for aboveground plant communities while the associated pattern of change in microbial communities remains largely unknown. A study was conducted along developmental sand-dune chronosequences bordering Lake Michigan at Wilderness State Park and Altamaha river valley of southeast Georgia with the hypothesis that soil bacterial communities will follow a pattern of change that is associated with soil, plant, and ecosystem development. This study site included 5 replicate sites along 14 dunes ranging in age from 105 to 500,000 years since deposition. The microbial composition and diversity in the soil was studied using …


A Morphological And Genetic Study Of Taxonomy And Evolutionary Divergence In Xanthisma Gracile And Xanthisma Spinulosum, Lavanya Challagundla May 2013

A Morphological And Genetic Study Of Taxonomy And Evolutionary Divergence In Xanthisma Gracile And Xanthisma Spinulosum, Lavanya Challagundla

Theses and Dissertations

Discerning the basis of phenotypic and genotypic differences within and between taxa is crucial for understanding the evolution of species, subspecies or varieties and races. In this dissertation, I have presented three studies, which use morphological characters and genetic Amplified Fragment Length Polymorphisms (AFLPs) to differentiate cytotypes, populations and species of the genus Xanthisma. The first study is aimed at clarifying the species status of Haplopappus ravenii, which has been considered to be a separate species by some taxonomists and a race of Xanthisma gracile by other researchers. Considering the morphological species concept and the genotypic cluster definition of a …


Microhabitat Use By Translocated Wild Turkeys In The Mississippi Delta, Matthew Ryan Mckinney May 2013

Microhabitat Use By Translocated Wild Turkeys In The Mississippi Delta, Matthew Ryan Mckinney

Theses and Dissertations

Eastern wild turkeys (Meleagris gallapavo silvestris) were extirpated from most of the Mississippi Alluvial Valley (MAV) by 1980 by overharvest and habitat loss. Federal programs have restored areas of the MAV to hardwood forest, potentially warranting restoring wild turkeys to the MAV. As part of a pilot restoration study, I gathered data on resource use and nest sites from July 2010–August 2011. I observed intraspecific niche specialization and spatial niche separation between genders. Spatiotemporal variation in resource availability and intraspecific competition appear to be factors influencing intraspecific niche specialization. Mature hardwood forest was primary habitat selected by translocated wild turkeys. …


Neural Protection In The Central Nervous System Against Nerve Agent Surrogates Using Novel Pyridinium Oximes, Ronald B. Pringle May 2013

Neural Protection In The Central Nervous System Against Nerve Agent Surrogates Using Novel Pyridinium Oximes, Ronald B. Pringle

Theses and Dissertations

Organophosphates (OPs), including nerve agents, target the cholinergic system via inhibition of acetylcholinesterase (AChE), with subsequent overstimulation resulting in neural damage and potential detrimental long-term effects. The efficacy of novel pyridinium oxime reactivators, created with moieties to increase blood-brain barrier penetration, was tested using highly relevant sarin and VX surrogates. Glial fibrillary acidic protein (GFAP; an indicator of neural damage) and monoamines (dopamine, serotonin, and their metabolites) were measured in select brain regions via immunohistochemistry and HPLC, respectively. Adult male rats were treated ip with high, sub-lethal doses of surrogates for sarin or VX, nitrophenyl isopropyl methylphosphonate (NIMP) or nitrophenyl …


Nutritional Profile Of Native Warm-Season Grass Grown As A Mono- Or Multi-Species Pasture, Babatunde Oloyede May 2013

Nutritional Profile Of Native Warm-Season Grass Grown As A Mono- Or Multi-Species Pasture, Babatunde Oloyede

Theses and Dissertations

The objective of this study was to evaluate the nutritional profile of mono- or multi-species pastures of native warm season grasses. One of four treatments were randomly assigned to Twelve pastures: 1) BG; 2) IG; 3) Mix G; 4) Mix NG. Growing steers (n = 225) were randomly assigned to one of nine pastures. Grass samples were taken from all pastures every 28 days during a four-month period and were analyzed for nutrient composition. Bermudagrass pastures had greater crude protein and ADF, but less NDF concentrations compared with the native warm-season grasses. Crude protein, IVDMD, and NDF IVDMD concentration decreased …


Salt Marsh Sediment Biogeochemical Response To The Bp Deepwater Horizon Blowout (Skiff Island, La, And Cat Island, Marsh Point And Saltpan Island, Ms), Calista Lee Guthrie May 2013

Salt Marsh Sediment Biogeochemical Response To The Bp Deepwater Horizon Blowout (Skiff Island, La, And Cat Island, Marsh Point And Saltpan Island, Ms), Calista Lee Guthrie

Theses and Dissertations

The impact of the Deepwater Horizon blowout on coastal wetlands can be understood through investigating carbon loading and microbial activity in salt marsh sediments. Carbon influx causes pore water sulfide to increase in wetland sediment, making it toxic and inhospitable to marsh vegetation. High sulfide levels due to increased microbial activity can lead to plant browning and mortality. Preliminary analyses at Marsh Point, Mississippi indicated that sulfate reducing bacteria are more active in contaminated marsh, producing sulfide concentrations 100x higher than in noncontaminated marsh. Sediment electrode profiles, hydrocarbon contamination, and microbial community profiles were measured at three additional locations to …


Spatial And Temporal Distribution Of Microbial Pathogens In Poultry Litter And The Development Of Microbial Inactivation Constants In Waste Application, Brandy Nicole Roberts May 2013

Spatial And Temporal Distribution Of Microbial Pathogens In Poultry Litter And The Development Of Microbial Inactivation Constants In Waste Application, Brandy Nicole Roberts

Theses and Dissertations

The increase in production farming, also known as concentrated animal feeding operations (CAFOs), garners more investigations on the implications to public health regarding the disposal of the wastes of food production animals. In addition to the vast amount of animal manure produced, human biosolids is another waste residual that must be managed. The research focus was the sustainability of foodborne pathogens in waste products and the variables that manipulate these environments such as moisture, temperature, organic matter and time. The first study was designed to analyze spatial differences in microbial populations in broiler litter by investigating the relationship of intra-house …


Evaluation Of The Impact Of The Deepwater Horizon Oil Spill And Hurricanes On Wildfires In Southeast Louisiana, Richard Carley May 2013

Evaluation Of The Impact Of The Deepwater Horizon Oil Spill And Hurricanes On Wildfires In Southeast Louisiana, Richard Carley

Theses and Dissertations

Remotely sensed MODIS fire detections were used to examine wildfire variability from 2003-2011 in southeast Louisiana, and to determine if the Deepwater Horizon oil spill and hurricanes may have impacted fire frequency and intensity. Despite low wildfire detection rates, around 60% for fires at least 1 km2 or greater, the MODIS fire product provided a consistent and reliable source of wildfire data. As a result of Hurricane Katrina, wildfire detection frequencies doubled their average numbers during the fall of 2005 in inland areas and during the spring of 2006 in inland and coastal marsh areas. Strangely enough, the oil spill …


Examining The Role Of L-Arginine In Tissues Of The Fetoplacental Unit And Endometrium, Jonathan Michael Greene May 2013

Examining The Role Of L-Arginine In Tissues Of The Fetoplacental Unit And Endometrium, Jonathan Michael Greene

Theses and Dissertations

L-arginine is one of the most versatile amino acids due to the fact that it serves as a precursor for many molecules which have important roles in bodily functions including mammalian reproduction. The current studies sought to further examine the role that L-arginine has in mammalian reproduction utilizing both in vivo and in vitro approaches. In the first study, a novel bioluminescent murine pregnancy model was developed to monitor VEGFR2 transcription activity non-invasively in the fetoplacental unit. Secondly, the effect that dietary L-arginine supplementation has during mouse gestation was examined. L-arginine supplementation increased weight gain during the latter third of …


Fermentation Of Glycerol To Biogas Under Isobaric And Variable Pressure Conditions, Benjamin P. Hartenbower May 2013

Fermentation Of Glycerol To Biogas Under Isobaric And Variable Pressure Conditions, Benjamin P. Hartenbower

Theses and Dissertations

With consideration to the crude glycerol surplus driven by the growth in biodiesel manufacturing, alternative uses for biodiesel derived glycerol have become increasingly essential. Anaerobic digestion of the glycerol reduces the chemical oxygen demand of the glycerol waste product, while capturing biogas as an energy source that can be used on site to reduce heating cost at a biodiesel facility. In this thesis, kinetic parameters are extracted from batch experimental data and applied to steady state equations. A flow sheet for the application of anaerobic digestion to biodiesel derived glycerol is developed and explored. The economic analysis of the scenario …


Forest Stand Preference Of Sirex Nigricornis, And Sirex Noctilio Hazard In The Southeastern United States, Kevin D. Chase May 2013

Forest Stand Preference Of Sirex Nigricornis, And Sirex Noctilio Hazard In The Southeastern United States, Kevin D. Chase

Theses and Dissertations

The Eurasian wood wasp, Sirex noctilio, is considered a secondary pest in its native range; however, it has caused significant economic damage when introduced to pine plantations in the Southern Hemisphere. Sirex noctilio was recently introduced to the northeastern U.S., which has raised concerns about its potential impact on Southeastern pine plantations. This research was conducted to understand how silvicultural management affects populations of a native wood wasp, Sirex nigricornis, a wood wasp with similar ecosystem functions as S. noctilio. Sirex nigricornis abundance was higher in un-managed pine plantations than in managed plantations, mixed, and old growth forests. Additionally, geospatial …


Genetic Insight Into The Function Of Pcp1p, A Mitochondrial Rhomboid Protease, Ningyu Xiao May 2013

Genetic Insight Into The Function Of Pcp1p, A Mitochondrial Rhomboid Protease, Ningyu Xiao

Theses and Dissertations

Rhomboid peptidases are conserved intramembrane serine proteases with mitochondrial family members being involved in mitochondrial dynamics and apoptosis. The Saccharomyces cerevisiae mitochondrial rhomboid, Pcp1p, catalyzes the cleavage of two substrates: Ccp1p, which breaks down reactive oxygen species, and Mgm1p, a GTPase mediating mitochondrial fusion events. As an initial approach to determine the structural basis of Pcp1p activity, a screen to identify temperature sensitive alleles of PCP1 was performed using hydroxylamine mutagenesis. Eight mutants were identified from a pool of 30,000 colonies that exhibited either temperature sensitive growth or respiratory defects. These mutants also exhibited defects in Mgm1p and Ccp1p processing …