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This Too Shell Pass: Investigating The Function Of B Cells During An Immune Response In The Red-Eared Slider, Trachemys Scripta, Christen Renee Fairow Jun 2023

This Too Shell Pass: Investigating The Function Of B Cells During An Immune Response In The Red-Eared Slider, Trachemys Scripta, Christen Renee Fairow

Theses and Dissertations

B cells are crucial components of the immune system during an infection that produce antibodies. It is not known how B cells function or if different cell subsets exist in reptiles. Understanding how B cells function in reptiles can help protect endangered species by clarifying how their immune system may be different from other vertebrates but may also allow for the development of new human medicines. First, we aimed to identify turtle leukocytes based on their morphological characteristics. Blood smears were made and photos were taken on a light microscope equipped with a digital camera. Using this method, we were …


Interpreting Communally: How Service Learning Impacts Interpreting Proficiency, Breanna Michele Jones Jun 2023

Interpreting Communally: How Service Learning Impacts Interpreting Proficiency, Breanna Michele Jones

Theses and Dissertations

Experiential learning programs, such as service learning, help students in Spanish medical interpreting classes to increase their interpreting proficiency, which includes their cultural understanding, linguistic performance, and professionalization (Rudvin & Tomassini, 2011). Currently, the Spanish Medical Interpretation class at Brigham Young University uses a community service-learning project in which students volunteer six hours of their time interpreting within the community. While largely beneficial, not all community partners offer the same learning opportunities. Some include training and shadowing programs, while others do not. This thesis seeks to determine the impact that shadowing opportunities and community partnerships have on interpreting proficiency and …


The Effects And Reversibility Of Combination Inhaled Corticosteroids On Phonation Threshold Pressure (Ptp) And Phonation Threshold Flow (Ptf) In Ex Vivo Rabbit Larynges, Melanie Elizabeth Blauer Jun 2023

The Effects And Reversibility Of Combination Inhaled Corticosteroids On Phonation Threshold Pressure (Ptp) And Phonation Threshold Flow (Ptf) In Ex Vivo Rabbit Larynges, Melanie Elizabeth Blauer

Theses and Dissertations

Although combination inhaled corticosteroids (ICs) are known to cause voice disorders in otherwise vocally healthy individuals, it is unknown whether those adverse effects can be reversed by the cessation of treatment. Quantitative aerodynamic measures such as phonation threshold pressure (PTP) and phonation threshold flow (PTF) can be used to identify the development of, and recovery from, vocal pathologies. We examined the effects and reversibility of ICs on laryngeal aerodynamics. This study was a mid-project investigation as part of a larger ongoing project. The 18 larynges were from rabbits that received ICs, a control condition, or no treatment. Experimental group rabbits …


Exploring Posttraumatic Growth In Aphasia: A Qualitative Investigation, Camille Williams Jun 2023

Exploring Posttraumatic Growth In Aphasia: A Qualitative Investigation, Camille Williams

Theses and Dissertations

This study explored posttraumatic growth (PTG) in people with aphasia and is the qualitative arm of a larger mixed-methods study. After an initial experimental session, 23 people with aphasia (PWA; 12 females and 11 males) completed a 60-minute semi-structured interview during which participants expanded on previously-given questionnaire responses and then answered five additional open-ended questions about communication in their daily life. Interviews were transcribed orthographically and coded using reflexive codebook analysis. A stakeholder with aphasia was consulted to gain insight from an insider's perspective about whether codes and themes captured the experiences of aphasia. Reflexive codebook analysis revealed two themes. …


Understanding The Experiences Of Mothers Of Children With Stunting In Two Egyptian Communities Focusing On The First 1000 Days, Rana Abdelgawad Jun 2023

Understanding The Experiences Of Mothers Of Children With Stunting In Two Egyptian Communities Focusing On The First 1000 Days, Rana Abdelgawad

Theses and Dissertations

Introduction: Egypt’s stunting prevalence is 13% with multiple initiatives and programs tackling child malnutrition. While many of them are promising, not all address the child’s first 1000 days, in addition to missing the cultural aspect for the Egyptian population which affects their dietary practices and health seeking behaviors.

Goal: Determine what challenges mothers face for their child’s nutrition and what learnings from exemplars countries can help Egypt reduce the malnutrition and stunting problem

Aim: Finding insights about the factors influencing child nutrition during 1st 1000 days and what programs can be implemented based on successful past experiences in exemplar countries. …


The Reversibility Of Effects Of Combination Inhaled Corticosteroids On Sustained Phonation Pressure And Flow In Ex Vivo Rabbit Larynges, Elisabeth Barlow Jun 2023

The Reversibility Of Effects Of Combination Inhaled Corticosteroids On Sustained Phonation Pressure And Flow In Ex Vivo Rabbit Larynges, Elisabeth Barlow

Theses and Dissertations

The purpose of this thesis is to investigate the reversibility of the adverse effects of combination inhaled corticosteroids (ICs) on vocal fold health as part of a five-year study. This pilot study tested the hypothesis that the adverse effects of ICs may be induced and then reversed, using a benchtop model and rabbit larynges measuring sustained subglottic pressure and airflow. Eighteen rabbits were assigned randomly to 5 subgroups, including baseline, induction experimental, induction control, reversibility experimental, and reversibility control. Baseline rabbits did not receive any treatment. Both experimental groups, induction and reversibility, were administered Advair until visual-perceptual ratings were noted. …


Neurodivergent College Students And Therapy Dogs In Higher Education, Georgia Jean Majka Jun 2023

Neurodivergent College Students And Therapy Dogs In Higher Education, Georgia Jean Majka

Theses and Dissertations

The purpose of this study is to examine neurodivergent students in higher education and their experience with The Shreiber Family Pet Therapy Program at Rowan University to investigate whether therapy dogs reduce anxiety levels and provide relaxation. In addition, this study provides recommendations for The Shreiber Family Pet Therapy program at Rowan University to promote the program and its purpose on campus. These resources are important when discussing the neurodivergent student population in higher education, which this research study discusses, based on previous research and the results found from this quantitative approach. This study also makes recommendations for Rowan University …


Examining The Validity Of The Cubed Narrative Language Measures Listening And Reading Subtests, Trevor David Read Jun 2023

Examining The Validity Of The Cubed Narrative Language Measures Listening And Reading Subtests, Trevor David Read

Theses and Dissertations

Purpose: The purpose of this study was to examine the concurrent and predictive validity of the CUBED Narrative Language Measures Listening and Reading subtests to evaluate their psychometric properties as defined by Deno (2003). Method: Evidence of concurrent validity for the NLM Listening and Reading subtests was examined using data from 1,146 preschool through third grade students. The NLM subtests were compared to similar subtests from other assessments administered at approximately the same time. Evidence of predictive validity was examined using data from these same NLM subtests from 1,512 kindergarten through third grade students, including 71 third grade students for …


“Tribal Rights Are Important Rights”: The Origins, Travails, And Impact Of The Nanticoke Lenni-Lenape Tribe V. The State Of New Jersey, Brianna Marie Dagostino Jun 2023

“Tribal Rights Are Important Rights”: The Origins, Travails, And Impact Of The Nanticoke Lenni-Lenape Tribe V. The State Of New Jersey, Brianna Marie Dagostino

Theses and Dissertations

The purpose of this thesis was to study the lawsuit case of the of Nanticoke Lenni-Lenape Tribal Nation V. John J. Hoffman and to showcase how modern-day racism ultimately led to their federal lawsuit in 2015. Racism and racist biases over Indian gaming has affected not just the Nanticoke Lenni-Lenape tribe, but tribes all over the country and has severely hindered tribes in the state and federal acknowledgment process. There are also other tribes that have had lawsuits over racial biases of Indian gaming, which will be discussed within the thesis. By using oral histories from tribal members and allies …


Investigating Eco-Evolutionary Interactions Between Hosts And Members Of Their Gut Microbiota, Logan A. Sauers Jun 2023

Investigating Eco-Evolutionary Interactions Between Hosts And Members Of Their Gut Microbiota, Logan A. Sauers

Theses and Dissertations

Evolutionary and ecological interactions between hosts and their associated microbial communities, their microbiota, and between members of these communities are vital to understand. Microbial communities are widespread across diverse host taxa and hosts receive a variety of well-documented benefits from their microbial communities. Despite the importance of understanding eco-evolutionary dynamics for colonization outcomes and the benefits these communities provide to their hosts, our current knowledge in this area remains incomplete. For example, we do not know the full extent of coevolution and specific relationships between hosts and microbes, and between the microbes themselves, across host taxa. Questions remain about how …


Transition Planning And The School To Prison Pipeline: A Phenomenological Study Investigating The Lived Experiences Of Alternatively Placed High School Black Males With High Incidence Disabilities, Brandon Garrett Thornton Jun 2023

Transition Planning And The School To Prison Pipeline: A Phenomenological Study Investigating The Lived Experiences Of Alternatively Placed High School Black Males With High Incidence Disabilities, Brandon Garrett Thornton

Theses and Dissertations

The purpose of this qualitative study was to better inform researchers and practitioners on best practices to ensure that their transition planning practices are person-centered and culturally responsive. Using a phenomenological approach, purposeful sampling was used to conduct semi-structured interviews regarding the shared educational lived experiences of two alternatively placed Black high school males with high incidence disabilities with respect to their transition planning. Participants perceived their transition planning to be student-centered, and expected favorable employment outcomes. Additionally, the participants’ choice to be alternatively placed played a role in their perception of transition outcomes. School personnel also played a pivotal …


The Effect Of The Carbon Nanotubes’ Surface Area On The Morphology And Performance Of Cellulose Acetate Nanocomposite Membranes In Nanofiltration Applications, Nouran Elbadawi Jun 2023

The Effect Of The Carbon Nanotubes’ Surface Area On The Morphology And Performance Of Cellulose Acetate Nanocomposite Membranes In Nanofiltration Applications, Nouran Elbadawi

Theses and Dissertations

Population increase and climate change have significantly affected the availability of fresh water for consumption. Studies by UNESCO showed that more than 28% of the world population didn’t have access to clean safe drinking water in 2020. This situation is expected to get more serious over the coming 30 years with the limited availability of natural fresh water sources. The need to find solutions from alternative non-freshwater resources for drinking or irrigation is becoming a necessity. Within the context of desalination technologies, thousands of investigations are conducted to find the cheapest most efficient way of water treatment aiming at expanding …


An Ambiguous Hermeneutic: Doubleness In Ingmar Bergman’S Quest For Self, Ingy Aziz Jun 2023

An Ambiguous Hermeneutic: Doubleness In Ingmar Bergman’S Quest For Self, Ingy Aziz

Theses and Dissertations

One of the functions of art in all its forms is to provide the means for self-exploration and, in this way, to enable us to relate cultural representation to the question of meaning. The beauty of cinematic art is that it gives voice to our deepest and most profound concerns and enables us to bridge the gap between personal psychology and public understanding. As interpreters, we do not always unearth the answers that we seek, but we certainly gain more insight through delving into the minds of major filmmakers in the canon of modern cinema. This thesis is on the …


Catecholamine System Function Of The Medial Prefrontal Cortex Following Repetitive Mild Traumatic Brain Injury And The Use Of Benzoyl Chloride To Quantify And Identify Neurotransmitters, Leah Horvat May 2023

Catecholamine System Function Of The Medial Prefrontal Cortex Following Repetitive Mild Traumatic Brain Injury And The Use Of Benzoyl Chloride To Quantify And Identify Neurotransmitters, Leah Horvat

Theses and Dissertations

Traumatic brain injury (TBI) is a serious global health burden that causes a wide range of neurocognitive symptoms. These cognitive processes are regulated by catecholamine signaling within the prefrontal cortex (PFC) region of the brain. To quantify functional deficits in catecholamine signaling after TBI, norepinephrine (NE) and dopamine (DA) levels within the PFC were quantified from microdialysate samples using high-performance liquid chromatography coupled with electrochemical detection. A method for benzoyl chloride derivatization of neurotransmitters and analysis with liquid chromatography and tandem mass spectrometry instrumentation was also developed to detect and analyze these neurotransmitters. Overall, it was found that catecholamine signaling …


Biopolymer-Coated Magnetite Nanoparticles For The Removal Of Emerging Contaminants From Aqueous Solutions, Hebatullah Farghal May 2023

Biopolymer-Coated Magnetite Nanoparticles For The Removal Of Emerging Contaminants From Aqueous Solutions, Hebatullah Farghal

Theses and Dissertations

Abstract

Biopolymers are gaining interest as promising materials for water purification due to their functionality, biodegradability, abundance, and safe use. In this work, three biopolymer-based systems were developed as adsorbents for removing emerging contaminants from water. The first system of biopolymeric nanocomposites was synthesized by coating chitosan/xylan biopolymeric nanocomposites on magnetite nanoparticles (CsXM) to facilitate their separation from water via a magnetic field. The prepared mesoporous CsXM nanocomposite was thermally stable and possessed a particle size of 11 nm and a neutral to slightly negative charge over the studied pH range of about 3 to 10. The nanocomposite efficiently removed …


Transfer Of Learning In Perception With Haptic Sensory Substitution Devices, Tyler Joseph Duffrin May 2023

Transfer Of Learning In Perception With Haptic Sensory Substitution Devices, Tyler Joseph Duffrin

Theses and Dissertations

Studies have shown that perceiving a given property of a wielded object requires task-specific sensitivity to the patterns of mechanical stimulation that support perceiving that property. The same is true for perceiving properties of the environment by means of a wielded object. Recently, studies using the Enactive Torch—a novel vibrotactile sensory-substitution device—have shown that these mechanical stimulation patterns are invariant across medium (Favela et al., 2018; 2021). The current study used a transfer of recalibration paradigm to the invariant patterns of mechanical stimulation that support perceiving surface distance by means of a wooden rod and by means of the Enactive …


Morphogenetic Engineering Of Synthetic Protocell Systems, Qinyu Zhu May 2023

Morphogenetic Engineering Of Synthetic Protocell Systems, Qinyu Zhu

Theses and Dissertations

Observing and studying how life forms behave, i.e., their movement, adaptability, and so on, have enabled human beings to develop new technologies or optimize existing ones. One of the more noticeable phenomena in Nature is morphogenesis. Morphogenetic processes exist in different stages of biological development, from cellular division to tissue and organ formation. It is easy to observe shape development during mophogenesis due to emerging imaging techniques. However, it is hard to understand this process due to its complex organization, and the morphogenetic responses can be induced by a variety of chemicals or mechanical stresses and are subject to the …


Multicultural Mentor Texts In 2nd Grade Writer’S Workshop, Ellen R. Carbonaro May 2023

Multicultural Mentor Texts In 2nd Grade Writer’S Workshop, Ellen R. Carbonaro

Theses and Dissertations

The purpose of this study is to investigate the effects of the utilization of relevant multicultural mentor texts during writer’s workshop instruction in the second-grade classroom. The specific aim is to understand how students relate these diverse texts to their development of literacy skills. This study is grounded in the theoretical frameworks of culturally relevant education and reader response theory. Upon data triangulation analysis, the following themes were identified: 1. Multicultural Literature Promoted Engagement with Literacy, 2. Multicultural Mentor Texts as a Guide for Writing, and 3. Writing as a Social Practice within the Writer’s Workshop Model. Implications regarding the …


Enhanced Throughput Of Aligned Polyacrylonitrile Nanofiber Manufacturing Via High-Speed Centrifugal Spinning, Jason Glen Ippolito May 2023

Enhanced Throughput Of Aligned Polyacrylonitrile Nanofiber Manufacturing Via High-Speed Centrifugal Spinning, Jason Glen Ippolito

Theses and Dissertations

Centrifugal spinning is noteworthy due to its high fiber throughput rate compared to other nanofiber manufacturing platforms. It is capable of producing fibers in the nanoscale diameter range from a multitude of polymers, such as polyacrylonitrile (PAN). With a traditional centrifugal spinner, fiber can be rapidly spun and collected on static posts. However, the use of static posts inevitably forms a dense ring of fiber after a short period of time, and this fiber build up can halt the spinning process. In this work, the factors that influence the throughput and scalability of highly aligned centrifugally spun PAN fibers are …


The Intolerance Of Uncertainty: Transdiagnostic Connections Across Schizophrenia And Mood Spectrums, Obsessive Beliefs, And Physical Health, Sharanjit D. Pujji May 2023

The Intolerance Of Uncertainty: Transdiagnostic Connections Across Schizophrenia And Mood Spectrums, Obsessive Beliefs, And Physical Health, Sharanjit D. Pujji

Theses and Dissertations

The ways in which we classify mental health symptoms are closely tied to etiological understandings and interventions. While prominent diagnostic systems are categorical in nature, dimensional and transdiagnostic conceptualizations may provide increased precision and specificity that are more representative of “real-world” symptom presentations. The intolerance of uncertainty (IOU), an aversive state formed in reaction to the fear of the unknown, may have transdiagnostic value across mental and physical health. The current study sought to examine dimensionality across traditionally distinct symptoms and the transdiagnostic role of IOU across physical and mental health through the lens of the schizophrenia spectrum with an …


Using Dual-Language Texts To Support Literacy For Emergent Bilingual Students, Amanda Dranchak May 2023

Using Dual-Language Texts To Support Literacy For Emergent Bilingual Students, Amanda Dranchak

Theses and Dissertations

This paper provides a qualitative account of how linguistically inclusive curriculums can enhance literacy instruction. The specific aim of this study is to explore the impact on literacy instruction of emergent bilingual students when the curriculum includes dual-language texts. Methods of data collection include student interviews, text discussions, student work, and student self-assessments. The findings show that the dual language literature positively affected the emergent bilingual students. Students enjoyed increased levels of engagement and a high level of confidence in their reading abilities. The research demonstrates the value of a culturally and linguistically inclusive instructional environment. With the inclusion of …


Identification And Quantification Of Allelochemicals Associated With Weed Suppression In Sweetpotato, Varsha Varsha May 2023

Identification And Quantification Of Allelochemicals Associated With Weed Suppression In Sweetpotato, Varsha Varsha

Theses and Dissertations

Mississippi ranks among the leading states in sweetpotato production in the US. However, managing weeds in sweetpotato fields presents a significant challenge, and the limited availability of herbicides labeled for use in sweetpotato makes it even more challenging. The widespread and repeated use of herbicides has led to the development of weed resistance. In addition, herbicides are incompatible with the organic production system. Finding sustainable weed management strategies to provide weed control options for organic cultivation and detain the development of herbicide-resistant weeds under conventional crop production is imperative. The natural chemicals released by the plants, also called allelochemicals, can …


Development And Evaluation Of An Automated Tactical Tillage Tool To Control Weeds In Row-Crop Production Systems, Grace Mccormick Friday May 2023

Development And Evaluation Of An Automated Tactical Tillage Tool To Control Weeds In Row-Crop Production Systems, Grace Mccormick Friday

Theses and Dissertations

Weed control is an integral part of a successful overall production strategy in row- cropping systems and has the potential to reduce or eliminate yield losses that negatively affect profitability. Timely and correctly selected herbicide applications are the major keys for effective weed control in a majority of instances. However, there are negative factors that contribute to ineffectiveness and weed escape issues that currently lack viable options for management. Sparsely populated late-season weeds that emerge after lay-by herbicide applications and weeds that have become tolerant and resistant to traditional herbicide chemistries are of greatest concern. Historically, these weeds would have …


Determining The Impact Of Post-Harvest Water Management On Chironomid Abundance, Agrochemical Biomass And Potential Trophic Biomagnification, Mason Thomas May 2023

Determining The Impact Of Post-Harvest Water Management On Chironomid Abundance, Agrochemical Biomass And Potential Trophic Biomagnification, Mason Thomas

Theses and Dissertations

Agriculture has diminished shorebirds’ natural habitat in the Mississippi Alluvial Valley. Remaining natural stopover sites are supplemented with agricultural fields during the fall and winter. This study evaluates the impact of 4 different post-harvest water management strategies on shorebird food abundance and potential agrochemical biomagnification. Chironomid samples estimated abundance, biomass, and chironomid agrochemical concentration in each field. A risk assessment of agrochemical biomagnification to shorebirds was made across all treatments. Of treatments represented on all study sites, winter treatment had greatest chironomid abundance and biomass. Models indicated that days since flood initiation, start date, and temperature are significant predictors of …


The Impact Of Environmental Education Delivery Methods And Outreach Messaging On Attitudes, Interests, And Intended-Behavior Changes Toward Nature, Vanessa Elizabeth Hoffman May 2023

The Impact Of Environmental Education Delivery Methods And Outreach Messaging On Attitudes, Interests, And Intended-Behavior Changes Toward Nature, Vanessa Elizabeth Hoffman

Theses and Dissertations

Environmental education (EE) is important for environmental awareness and stewardship. Involving youth and adults in EE ensures current and future generations will conserve natural resources. This thesis explores impacts of delivery methods on participants’ environmental attitudes and behaviors toward nature through middle school classroom- and camp-based EE instruction, focus group interviews with rural minority youth, and adult responses to aquatic conservation outreach messaging. I observed no difference in environmental attitudes between online versus in-person delivery of EE lessons for middle-schoolers. Outdoor-based learning may be more impactful for EE than experiential, classroom-based learning. Focus group outcomes suggested youth EE programs should …


Physiological And Proteomic Analysis Of Soybean In Response To Drought And Heat Stress, Amandeep Kaur May 2023

Physiological And Proteomic Analysis Of Soybean In Response To Drought And Heat Stress, Amandeep Kaur

Theses and Dissertations

Drought and temperature stress are the two most important factors limiting crop productivity. A physiological and proteomic analysis of soybean was performed to investigate the negative impact of these variables. The previous studies reported the beneficial effect of silicon under stress conditions. Silicon supplementation may promote plant growth under drought stress, although the mechanism to alleviate drought stress is uncertain. The proteomic analysis of chloroplast proteins was carried out to study the response of silicon supplementation under drought stress.

Similarly, temperature stress, mainly heat stress, has an adverse effect on soybean plants. The comparative analysis of two soybean cultivars, DS25-1 …


Development Of Live Attenuated A. Hydrophila Vaccines And Understanding Of Macrophage-Aeromonas Hydrophila Interactions, Eda Ozdemir May 2023

Development Of Live Attenuated A. Hydrophila Vaccines And Understanding Of Macrophage-Aeromonas Hydrophila Interactions, Eda Ozdemir

Theses and Dissertations

Channel catfish farming is the largest aquaculture industry in the U.S., and virulent Aeromonas hydrophila is an important re-emerging bacterial pathogen of farmed catfish. A. hydrophila is ubiquitous in the aquatic environment and causes motile Aeromonas septicemia (MAS) in farmed catfish. A. hydrophila infection is challenging to treat due to rapidly progressive mass mortalities. Although florfenicol has been used in managing outbreaks, acute onset of anorexia, drug cost, and emergence of antimicrobial-resistant strains are significant concerns. Prevention of MAS by vaccination could be a promising approach, but a commercial vaccine is not available. Thus, it is critical to develop effective …


Understanding Dietary And Thermal Influences On Invasive Cichlids In Puerto Rico Reservoir Systems, Jacob A. Moreland May 2023

Understanding Dietary And Thermal Influences On Invasive Cichlids In Puerto Rico Reservoir Systems, Jacob A. Moreland

Theses and Dissertations

Jaguar Guapote (Parachromis managuensis) and Amphilophus spp. were assessed for effects of temperature on standard metabolic rate (SMR) and lower dissolved oxygen tolerance (LDOT), efficacy of gastric lavage for diet analysis, and diet in Puerto Rico reservoirs. Fish were acclimated to 22°C, 28°C, and 34°C and SMR and LDOT were measured using intermittent respirometers. Jaguar Guapote acclimated to 34°C had the greatest SMR, with 22°C and 28°C having similar SMR values. Amphilophus spp. acclimated to 28°C were similar to fish acclimated at 22°C and 34°C. Fish were resilient to high temperatures, losing equilibrium more rapidly at the coldest temperature, 22°C. …


Eating German, The American Way: German And American Cooking Traditions, Potato Salad, And The Culinary Assimilation Of German Immigrants, 1820-1920., Scott Wooley May 2023

Eating German, The American Way: German And American Cooking Traditions, Potato Salad, And The Culinary Assimilation Of German Immigrants, 1820-1920., Scott Wooley

Theses and Dissertations

“Eating German, the American Way” explores how and why the mayonnaise-based potato salad came to be a staple of American culinary tradition. It examines how native-born Americans and German immigrants in the nineteenth century identified themselves based on their culinary traditions and what they ate and how the interactions between, and accessibility of, those traditions created a new identity based on the sharing of recipes as the two groups mingled and assimilated to each other. It uses food as a way to understand the processes of assimilation by defining the distinctions between the two groups based on their separate repertoire …


Molecular Characterization Of Seminal Plasma From Boars With Divergent Sperm Quality, Notsile Hleliwe Dlamini May 2023

Molecular Characterization Of Seminal Plasma From Boars With Divergent Sperm Quality, Notsile Hleliwe Dlamini

Theses and Dissertations

Seminal plasma (SP) is the microenvironment of spermatozoa whose composition reflects semen quality and constitutes an excellent source for detecting non-invasive biomarkers. This study characterizes good vs. poor quality semen using seminal extracellular vesicles coupled proteins and miRNA. Fresh boar semen samples were screened, centrifuged, and SP supernatants were collected for near-infrared spectroscopy (NIRS) analysis. EVs were extracted from SP (SP-EVs), characterized, and those of extremely poor or good sperm quality groups were subjected to proteomics and small RNA sequencing. Significant differences were set for P