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Full-Text Articles in Entire DC Network
Physiological And Yield Responses Of Soybean Cultivars To Heat And Drought Stresses, Sadikshya Poudel
Physiological And Yield Responses Of Soybean Cultivars To Heat And Drought Stresses, Sadikshya Poudel
Theses and Dissertations
Soybean (Glycine max L.) is an important legume crop often exposed to heat and drought stresses during reproductive and early-seed setting stages, resulting in lower yields and seed quality. Ten soybean cultivars were phenotyped for individual (drought or heat) and combined stress tolerance. Under drought, reduced stomatal conductance and increased canopy temperature significantly reduced seed number (46%) and weight (35%). Heat stress alone reduced seed number (19%) and weight (23%) compared to control. Moreover, a degree increase in daytime temperature above 32 °C during the reproductive stage reduced seed weight by 4% and 7% under well-watered and drought conditions, respectively. …
Evaluation Of Conservation Practices Effect On Water Quality Using The Swat Model, Vivek Venishetty
Evaluation Of Conservation Practices Effect On Water Quality Using The Swat Model, Vivek Venishetty
Theses and Dissertations
The deterioration of water quality due to human-driven alternations has an adverse effect on the environment. More than 50% of surveyed surface water bodies in the United States (US) are classified as impaired waters as per the Clean Water Act. The pollutants affecting the water quality in the US are classified as point and non-point sources. Pollutant mitigation strategies such as the selective implementation of best management practices (BMPs) based on the severity of the pollution could improve water quality by reducing the amounts of pollutants. Quantifying the efficiency of a specific management practice can be difficult for large watersheds. …
Interspecies Differences In Organophosphate Anticholinesterase Inhibition Potency And Reactivation Using Novel Oximes, Katie Elizabeth Strickland
Interspecies Differences In Organophosphate Anticholinesterase Inhibition Potency And Reactivation Using Novel Oximes, Katie Elizabeth Strickland
Theses and Dissertations
Organophosphates are insecticides which result in acute adverse signs when exposed at toxic doses by animals and lead to death if left untreated. The current treatment for organophosphate toxicity includes atropine and the federally approved oxime 2-PAM. However, 2-PAM is not very effective at crossing the blood brain barrier which results in prolonged inactivation of acetylcholinesterase, which is the primary target of organophosphates, in the brain even after administration. The novel oximes, Oxime 15 and Oxime 20, are able to cross the blood brain barrier and reactivate the inhibited acetylcholinesterase. In this experiment with six animal species frequently used in …
Evaluation Of The Relationship Between Hair Coat Shedding Ability, Apparent Forage Digestibility, And Mineral Status In Angus Cattle, Margaret P. Loftin
Evaluation Of The Relationship Between Hair Coat Shedding Ability, Apparent Forage Digestibility, And Mineral Status In Angus Cattle, Margaret P. Loftin
Theses and Dissertations
The objective of this study was to evaluate the relationship between hair coat shedding ability, apparent forage digestibility, and mineral status in Angus cattle. Fecal samples were collected on fall calving Angus cows for one week in March, May, and July from 2019-2022. Liver and plasma samples were collected in March and July each year. Fecal samples were analyzed for DM, ash, NDF, and ADF while liver and plasma were analyzed for mineral concentration. Data were analyzed using MIXED procedure of SAS with significance declared at P0.05. Interactions were detected for DM, NDF, ADF, and liver Fe concentration. The model …
Effects Of Sampling Methodologies On Mycoplasma Gallisepticum Tissue Populations In Commercial Layer Pullets, Sethulakshmi Kattupparayil Sasidharan
Effects Of Sampling Methodologies On Mycoplasma Gallisepticum Tissue Populations In Commercial Layer Pullets, Sethulakshmi Kattupparayil Sasidharan
Theses and Dissertations
Mycoplasma gallisepticum (MG) can cause chronic respiratory disease in chickens and infectious sinusitis in turkeys. Impacts of MG infections can include increased morbidity and mortality, decreased egg production, hatchability and feed efficiency. Biosecurity and bio-surveillance remain the primary means of deterrence as the current means of control are not wholly effective. Further applications towards the characterization of MG-related disease control requires proper understanding of the characteristics of MG infections with accurate and efficient quantification of in vivo MG populations. To this end, study was conducted to determine the in vivo MG populations in infected pullets and to determine impact of …
Impact Of Daily Sweet Potato Ingestion On Alterations To The Gut Microbiome, Cholesterol, And Exercise Performance, Johneric William Smith
Impact Of Daily Sweet Potato Ingestion On Alterations To The Gut Microbiome, Cholesterol, And Exercise Performance, Johneric William Smith
Theses and Dissertations
The importance of the gut microbiome is being explored in relation to multiple facets of health and performance. The purpose of this study was to explore the impact of frequent sweet potato ingestion on alterations of the gut microbiome which might in turn alter cholesterol levels and exercise performance. Thirteen recreationally trained males had fecal and blood samples collected and exercised to fatigue with and without exogenous carbohydrate ingestion prior to and following 42 days of daily sweet potato ingestion. Bacterial communities were extracted from fecal samples and bacterial DNA were sequence. Blood samples were analyzed for total cholesterol, low-density …
Evaluation Of Nutrient Digestibility Of Weaned Calves From Early And Late Shedding Dams, Jennifer Keele
Evaluation Of Nutrient Digestibility Of Weaned Calves From Early And Late Shedding Dams, Jennifer Keele
Theses and Dissertations
Researchers have investigated several factors that could alter fetal growth, including nutrient restriction (Valiente et al., 2021), hair shedding (Gray et al., 2011), and extreme hot and cold temperatures (Davidson et al., 2022). Hot temperatures and increased humidity percentages in the southeast United States caused researchers to investigate the hair coats of Angus cattle in the commercial production setting. An improvement in fiber digestibility and calf birth and weaning weights has been observed in Angus dams that shed 50% of the winter hair coat by May (Gray et al., 2011; Burnett et al., 2021). Our objective of this experiment was …
Marxist Analysis Of Social And Economic Narratives In Childrens' Cartoons, Shane Mcgregor
Marxist Analysis Of Social And Economic Narratives In Childrens' Cartoons, Shane Mcgregor
Theses and Dissertations
Using a Marxist framework with a grounding in critical literacy, this study employs a content analysis methodology to analyze 25 episodes of five of the most popular children’s television cartoons in order to understand how these cartoons portray economic and social systems, as well as how the messages these cartoons express would tend to support these systems. In so doing, this research hopes to provide a conceptual framework that educators and parents can use as a guide for demonstration of a critical approach to understanding the curriculum of children’s media inside or outside of the classroom. Educators can modify this …
The Neuroprotective Role Of Vitamin D In Humans With A Traumatic Brain Injury: A Systematic Review, Hailey Jarr
The Neuroprotective Role Of Vitamin D In Humans With A Traumatic Brain Injury: A Systematic Review, Hailey Jarr
Theses and Dissertations
Traumatic brain injuries (TBI) are a leading cause of death worldwide. Previous research supports a relationship between vitamin D and TBI outcomes; however, they rely on animal studies. This review aimed to examine the neuroprotective role of vitamin D in humans with TBIs. A search was completed (PubMed and Scopus) following PRISMA guidelines to review publications investigating the relationship between vitamin D deficiency (VDD) and TBI or the effect of vitamin D supplementation on TBI outcomes; 8 studies were identified. Patients had the
/="/">following ranges: 26-88% deficiency and 62-88% insufficiency compared to 24% and 49% of the general population. …
Patterns Of Distribution And Dispersion Of Silver Carp In An Oxbow Lake, Jordan Besson
Patterns Of Distribution And Dispersion Of Silver Carp In An Oxbow Lake, Jordan Besson
Theses and Dissertations
Oxbow lakes are highly productive waterbodies that host multiple life stages of many freshwater aquatic species. Oxbow lakes also provide habitat to Silver Carp (Hypophthalmichthys molitrix), which has enabled populations to grow and expand within the United States. Silver Carp are undesirable because they can compete for resources with native fishes. My goal was to identify patterns of distribution and dispersion of Silver Carp in Moon Lake, Mississippi, to assist and inform precise harvesting of fish. I implanted thirty-five adult Silver Carp with acoustic tags that I released into Moon Lake. I observed that Silver Carp were disproportionately …
Identifying Limitations In Using Diagnostic Testing For Absorption Of Passive Maternal Immunity In Neonatal Beef Calves To Predict Pre-Weaning Disease, Alexis Charlotte Thompson
Identifying Limitations In Using Diagnostic Testing For Absorption Of Passive Maternal Immunity In Neonatal Beef Calves To Predict Pre-Weaning Disease, Alexis Charlotte Thompson
Theses and Dissertations
Calves are born agammaglobulinemic and rely on colostrum consumption for the transfer of maternal passive immunity. Calves that fail to absorb adequate amounts of maternal antibodies from colostrum are commonly referred to as having failed transfer of passive immunity (FTPI). The overall aim of this dissertation was to explore the usefulness of FTPI testing in neonatal beef calves to predict their risk for subsequent illness or death. The objectives were to evaluate the impact of FTPI on pre-weaning disease in beef and dairy calves, quantify and compare the variance in IgG concentrations measured by radial immunodiffusion and serum total protein …
Marginal Agricultural Land Identification In The Lower Mississippi Alluvial Valley, Prakash Tiwari
Marginal Agricultural Land Identification In The Lower Mississippi Alluvial Valley, Prakash Tiwari
Theses and Dissertations
This study identified marginal agricultural lands in the Lower Mississippi Alluvial Valley using crop yield predicting models. The Random Forest Regression (RFR) and Multiple Linear Regression (MLR) models were trained and validated using county-level crop yield data, climate data, soil properties, and Normalized Difference Vegetation Index (NDVI). The RFR model outperformed MLR model in estimating soybean and corn yields, with an index of agreement (d) of 0.98 and 0.96, Nash-Sutcliffe model efficiency (NSE) of 0.88 and 0.93, and root mean square error (RMSE) of 9.34% and 5.84%, respectively. Marginal agricultural lands were estimated to 26,366 hectares using cost and sales …
The Effect Of Vitamin B-12 Supplementation On Visual Reaction Time In Young Adult Females, Sean M. Servalish
The Effect Of Vitamin B-12 Supplementation On Visual Reaction Time In Young Adult Females, Sean M. Servalish
Theses and Dissertations
This study investigated the effect of vitamin B-12 supplementation on visual response time (VRT). Menstrual cycle phase (MCP) and 24-hour dietary intake of B-12 were also observed as dependent variables. This blind, placebo-controlled trial observed 14 young adult females in four treatment groups: control, 500mcg (low dose), 2000mcg (moderate dose), and 3000mcg (high dose). The study lasted 28 days, with participants treated weekly, randomly assigned to each of the treatment groups. When comparing B-12 dose treatment groups to VRT baseline, there was significant improvements in VRT. Compared to the placebo, there were no significant differences among treatment groups. Significant differences …
An Investigation Into Transcription Fidelity And Its Effects On C. Elegans And S. Cerevisiae Health And Longevity, Olivia S. Dinep-Schneider
An Investigation Into Transcription Fidelity And Its Effects On C. Elegans And S. Cerevisiae Health And Longevity, Olivia S. Dinep-Schneider
Theses and Dissertations
mRNA molecules form an intermediate in the transfer of sequences from DNA to ribosomes in order to guide protein production. Errors can be introduced into mRNA, producing aberrant proteins which place a strain on cellular regulatory machinery, causing increased risks of apoptosis, cancer, and decreased fitness. These errors may be introduced due to decreased transcriptional proofreading capabilities, exposure to chemicals, or mistakes in RNA editing machinery. It is important to investigate these causes of transcription errors to better understand the long-neglected area of mRNA fidelity which has such significant impacts on our cellular functions. In this paper, it was determined …
Physiology Of Human-Equine Interaction During Substance Withdrawal, Molly Marie Friend
Physiology Of Human-Equine Interaction During Substance Withdrawal, Molly Marie Friend
Theses and Dissertations
Psychotherapy incorporating equine interaction (PIE) is emerging as an effective treatment for substance use disorder (SUD), but research is limited concerning physiological impacts during substance withdrawal. This study investigated impacts of PIE on salivary cortisol concentrations, heart rate, anxiety, and depression during SUD withdrawal. Heart rate and cortisol concentrations were measured in horses to determine potential human-horse coupling. Saliva samples and heart rates were collected pre and post PIE from residential SUD patients (n=18) and their therapy horses (n=4) during and following the withdrawal period. Participants (n=10) also completed a survey measuring anxiety and depression (P = 0.05). A …
Determining The Importance Of Propagule Pressure And Dispersal Mechanisms For The Establishment And Spread Of Crapemyrtle Bark Scale, Acanthococcus Lagerstroemiae Kuwana (Hemiptera: Eriococcidae), Erika Renee Wright
Theses and Dissertations
Non-native scale insects can be economically and ecologically important pests of urban forests. Crapemyrtle bark scale (CMBS), Acanthococcus lagerstroemiae Kuwana (Hemiptera: Eriococcidae), is a scale that causes declines in plant vigor for a popular ornamental tree in the southeastern United States, crapemyrtle (Lagerstroemia spp.). CMBS has spread rapidly throughout the Southeast and, more recently, into the Middle Atlantic. Despite problems associated with CMBS, important aspects of its ecology, such as the role of propagule pressure in establishment and the relative importance of mechanisms for between-tree dispersal, are not well-understood. We used field and laboratory studies to investigate these aspects of …
Roles Of Limestone Particle Size Ratios And Phytase Levels On Performance, Eggshell Quality, And Bone Health In Post-Peak And Late-Lay Single-Cycled W-36 Laying Hens, Charis Waters
Theses and Dissertations
The objective of this study was to investigate the role of fine (F) and coarse (C) limestone ratios and exogenous phytase at standard and superdosing activity on performance, egg quality, and bone quality of post-peak and late-lay Hy-Line W-36 hens. For post-peak, treatments with 40F:60C at 0 and 400 FTU/kg (standard phytase activity) increased hen-day egg production (HDEP). Treatments with 15F:85C decreased feed intake (FI) but also increased unsaleable eggs (UE) and eggshell-breaking strength (EBS). Treatments with 0 and 400 FTU/kg increased albumen quality and treatments with 1500 FTU/kg increased bone quality and phytate breakdown. For late-lay, interaction effects of …
Remote Physiological Monitoring Of The Giant Panda And Red Panda Using Near Infrared Spectroscopy And Machine Learning, Qingyu Sheng
Remote Physiological Monitoring Of The Giant Panda And Red Panda Using Near Infrared Spectroscopy And Machine Learning, Qingyu Sheng
Theses and Dissertations
Appropriate conservation decisions and efforts must be based on real−time and accurate information about wildlife populations. However, it is extremely challenging to monitor the population demography and physiological traits of many threatened and secretive animal species through direct observation and capture. Near infrared spectroscopy (NIRS) has the potential to be a remote tool to address questions concerning wildlife physiology and demography by analyzing “signs” of animals without seeing or capturing them. In this dissertation, two species, the giant panda (Ailuropoda melanoleuca) and red panda (Ailurus fulgens) are used as a case study, to demonstrate NIRS’ feasibility …
Wildfire-Altered Geotechnical Soil Properties And Their Impact On Subsequent Landslides, James M. Kellogg
Wildfire-Altered Geotechnical Soil Properties And Their Impact On Subsequent Landslides, James M. Kellogg
Theses and Dissertations
Wildfires result in altered landscape as well as altered soil properties. Wildfires result in conditions that are more susceptible to geotechnical hazards introducing a potential for cascading events in the surrounding area. Shallow landslides are common geohazards that result in a post-wildfire landscape. With an ever-increasing trend in wildfires, the need to better understand the impact wildfire-altered soil properties play on shallow slope instability is critical to evaluating risks to existing or planned development, mitigation of potentially hazardous conditions, prioritization of post-wildfire planning, and post-wildfire response preparation. The primary objective of this study is to provide a comprehensive overview of …
Identifying Barriers To Data Use On U.S. Beef Cow-Calf Operations And Developing Solutions To Improve Cow-Calf Record-Keeping, William Isaac Jumper
Identifying Barriers To Data Use On U.S. Beef Cow-Calf Operations And Developing Solutions To Improve Cow-Calf Record-Keeping, William Isaac Jumper
Theses and Dissertations
Cattle health and production records (CHPR) are data collected by cattle producers and veterinarians in the form of measurements, observations, counts of events over time, and physiologic attributes that describe individual and group-level health and production. These data are useful to both veterinarians and cattle producers for making evidence-based decisions on cow-calf operations. Currently, there are no uniform, industry-wide methods of capturing and recording CHPR in the U.S. cow-calf industry. Although many cow-calf producers in the U.S. are thought to collect some form of CHPR, it is believed that relatively few are doing so in an electronic manner that facilitates …
Northern Bobwhite (Colinus Virginianus) Breeding Season Calling Behavior And Roost Site Selection In A Working Agricultural Landscape In Clay County, Mississippi, Olivia A. Lappin
Northern Bobwhite (Colinus Virginianus) Breeding Season Calling Behavior And Roost Site Selection In A Working Agricultural Landscape In Clay County, Mississippi, Olivia A. Lappin
Theses and Dissertations
Research on northern bobwhite calling behavior and roosting ecology during the breeding season has received limited attention. However, variability in calling rate in response to conspecifics and environmental factors may affect avian monitoring strategies. We conducted 10-minute breeding season surveys (April-August) on B. Bryan Farms, Mississippi, from 2021-2022 to evaluate the impact of conspecifics and environmental covariates on male bobwhite calling rate. We observed a positive linear effect of conspecifics and temperature on calling rate. We did not find sufficient evidence to support a relationship between calling rate and cloud cover or barometric pressure. We also analyzed breeding season third-order …
Evolution And Epidemiology Of Channel Catfish Virus (Ccv), Arun Venugopalan
Evolution And Epidemiology Of Channel Catfish Virus (Ccv), Arun Venugopalan
Theses and Dissertations
Channel catfish virus disease (CCVD) is the principal viral disease in the United States catfish industry. The CCVD is caused by channel catfish virus (CCV), with mortality reaching up to 100% in fingerlings. CCV is assigned taxonomically to the family Alloherpesviridae, genus Ictalurivirus, species Ictalurid herpesvirus 1 (IcHV-1). To date, virulence, immunogenicity, and genome plasticity of the CCV field isolates have not been investigated. Three genotypes of CCV (IcHV-1A, IcHV-1B, and BCAHV) were identified using restriction fragment length polymorphism (RFLP) analysis. Virulence assessment of three representative isolates of RFLP groups suggests that IcHV-1B (pooled survival [mean ± SE]: 58.3% ± …
Macroecological Patterns Of Frugivorous Fishes’ Diversity (Serrasalmidae) In The Amazon Drainage Basin, Karold Vivianna Coronado Franco
Macroecological Patterns Of Frugivorous Fishes’ Diversity (Serrasalmidae) In The Amazon Drainage Basin, Karold Vivianna Coronado Franco
Theses and Dissertations
The Amazon River drainage basin is known as the most biodiverse region in the world. Regarding freshwater fish, this region contains around 15% of the world's fish richness. The fish family Serrasalmidae have a variety of feeding preferences including Pacus (herbivores) and Piranhas (carnivores). Fruit eating fish species are evolutionarily the oldest species in the family and have a mutualistic interaction with flooded forests. Considering the diversity of feeding habits of the Serrasalmidae family, it represents an excellent model to study ecological questions related to factors that influence the spatial distribution of species and factors that contribute to the maintenance …
The Influence Of Overwatering, Underwatering, And Waterlogging On The Growth Of Kale (Brassica Oleracea Var. Acephala), Skyler R. Brazel
The Influence Of Overwatering, Underwatering, And Waterlogging On The Growth Of Kale (Brassica Oleracea Var. Acephala), Skyler R. Brazel
Theses and Dissertations
The Intergovernmental Panel on Climate Change has predicted that there will be increases in precipitation and heat-induced drought events globally. Information on kale response to waterlogging is minimal. The purpose of this project was to identify the response of kale to three treatments of water stress: underwatering, overwatering, and waterlogging. Plant pigments analyzed displayed a varied response to underwatering and overwatering, with concentrations changing with maturity but with reductions and no changes, respectively, at full harvest maturity. Glucosinolate concentrations were also influenced by maturity and increased under waterlogging and underwatering but no differences with overwatering. Overall, water stress to any …
Surface Modification Of Wood Using Nano-Sized Titania Particles Coated By Liquid-Precursor Flame Spray Pyrolysis, Ganesh Sedhain
Surface Modification Of Wood Using Nano-Sized Titania Particles Coated By Liquid-Precursor Flame Spray Pyrolysis, Ganesh Sedhain
Theses and Dissertations
Wood is a renewable resource and versatile material used in tasks ranging from tools and furniture to advanced engineering structures. Although wood is light, mechanically robust, environmentally friendly, and abundant, some inherent properties of wood, such as degradation due to moisture and UV radiation from sunlight, are less desirable for extended service life and dimensional stability. In this dissertation, a novel surface modification of wood is explored by depositing nano-sized titania particles on wood veneers and cross-laminated timber (CLT) blocks by liquid-precursor flame spray pyrolysis to confer reversible wettability switching and enhanced durability to UV irradiation. The reaction between a …
Spatial Conservation Planning In The Southeastern United States: Alignments And Opportunities, Bradly Stewart Thornton
Spatial Conservation Planning In The Southeastern United States: Alignments And Opportunities, Bradly Stewart Thornton
Theses and Dissertations
Conservation managers and planners need the ‘best available science’ to support robust and defensible decisions, ensuring that public resources are appropriately allocated. Spatial planning products and decision-support tools developed for this purpose should enable partner organizations to achieve focus, coordination, and increased effectiveness in their investments and actions. Whereas conservation partnerships have historically created distinct planning tools, there is increasing interest for improved coordination, communication, and unifying biological datasets to improve the cohesiveness of regional management activities. We sought to inform spatial conservation planning efforts in the southeastern United States through the development of species distribution models for focal avian …
The Mississippi Timber Severance Tax: Its Economic Impacts To Forestry And The State Economy, Sakar Nepal
The Mississippi Timber Severance Tax: Its Economic Impacts To Forestry And The State Economy, Sakar Nepal
Theses and Dissertations
Millions of dollars are collected through Mississippi’s timber severance tax every year which then funds the Forest Resource Development Program (FRDP). This study analyzed their contributions to Mississippi’s economy and found that the total possible contribution was estimated to be $6.0 million in industrial output and 222 full-time and part-time jobs in 2019. However, only about 70 percent of the FRDP funds were expended in that year, and the actual contribution was short by $1.80 million in output and 80 full-time and part-time jobs. This study also examined the impact of the severance tax and FRDP on forest investment, using …
Host-Acquired Virus Genes Support An Ancient Antiviral Role Of The Pirna Pathway In Dipterans, Rebecca Christian
Host-Acquired Virus Genes Support An Ancient Antiviral Role Of The Pirna Pathway In Dipterans, Rebecca Christian
Theses and Dissertations
Endogenous viral elements (EVEs) have been recently investigated as a source of transgenerational immune memory. These “viral fossils” are abundant in Aedes mosquitoes and partner with the host’s primary antiviral defense system, the RNA interference (RNAi) pathways. This partnership appears unique to mosquitoes, which encode an expansion of the Piwi endoribonucleases. To interrogate EVE-Piwi partnerships and their role in antiviral defense, I performed a comparative small RNA analysis of two naturally occurring EVE-virus pairs – one in the mosquito Aedes albopictus, and one in the midge Chaoborus americanus. Both express an EVE related to the nucleoprotein of their …
Dead Men Tell No Tales: How The British Empire Destroyed Pirates With Monstrous Legal Rhetoric, Ashley L. Nef
Dead Men Tell No Tales: How The British Empire Destroyed Pirates With Monstrous Legal Rhetoric, Ashley L. Nef
Theses and Dissertations
The state often enacts violence against marginalized groups by rendering them monstrous. The early eighteenth century saw early and stellar instances of this phenomenon in the way the British Empire pursued and executed pirates. These "golden age" pirates represented an extraordinary cross-section of marginalization politically, economically, socially, and otherwise, all of which threatened the political and social mores of Imperial Britain. In order to implement a policy and practice of pirate annihilation, British authorities constructed pirates as monstrous by racializing, dehumanizing, and emphasizing the supernatural quality of pirates. This study analyzes three eighteenth-century piracy trial transcripts--those of William Kidd, Stede …
Developing Genotypic And Phenotypic Systems For Early Analysis Of Drug-Resistant Bacteria, Yesman Akuoko
Developing Genotypic And Phenotypic Systems For Early Analysis Of Drug-Resistant Bacteria, Yesman Akuoko
Theses and Dissertations
Antimicrobial resistance in bacteria is a global health challenge with a projected fallout of 10 million deaths annually and cumulative costs of over 1 trillion dollars by 2050. The currently available tools exploited in the detection of bacteria or their DNA can be expensive, time inefficient, or lack multiplex capabilities among others. The research work highlighted in this dissertation advances techniques employed in the phenotypic or genotypic detection of bacteria and their DNA. In this dissertation, I present polymethyl methacrylate-pressure sensitive adhesive microfluidic platforms developed using a time-efficient, inexpensive fabrication technique. Microfluidic devices were then equipped with functionalized monoliths and …