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On The Allelic Architecture Of Multiple Sclerosis In Sardinia, Athena Hadjixenofontos
On The Allelic Architecture Of Multiple Sclerosis In Sardinia, Athena Hadjixenofontos
Open Access Dissertations
Multiple Sclerosis (MS) is a demyelinating disease of the central nervous system with autoimmune etiology. It affects approximately 2.3 million people worldwide, but prevalence is distributed unequally with countries closer to the equator manifesting a lower prevalence of MS. The Italian island of Sardinia is an exception, with prevalence rates that are among the highest in the world. Sardinia is inhabited by a unique, isolated population that was founded approximately 10,000 years ago. The reasons for this enrichment of MS cases in Sardinia are unknown. Like most complex diseases, MS has both genetic and environmental components of susceptibility. To date, …
New Insights In The Regulation Of Mitochondrial Functions And Metabolic Reprogramming By Erbb Receptors In Cancer, Nirav Patel
New Insights In The Regulation Of Mitochondrial Functions And Metabolic Reprogramming By Erbb Receptors In Cancer, Nirav Patel
Open Access Dissertations
Dysregulation of ERBBs signaling has been known to be responsible for several types of tumors including breast, ovary, lung, pancreas, prostate and more. Functions of ERBB receptors are mainly known from its overexpression in cancers. Apart from elevated levels of ERBB receptors, low levels of ERBB signaling also play a crucial role in normal development and maintenance of mammalian organs. However, the role of signaling by the low “normal” levels of ERBB receptors is poorly understood. Altered ERBB signaling has been implicated with several cardiac defects. One of the severe side effects of ERBB2-targeted therapy in breast cancer patients is …
Fluorescence Photoactivation Based On 2-Nitrobenzyl Derivatives, Subramani Swaminathan
Fluorescence Photoactivation Based On 2-Nitrobenzyl Derivatives, Subramani Swaminathan
Open Access Dissertations
Fluorescence microscopy offers a non-invasive way to image biological species in real time. The phenomenon of diffraction, however, restricts the resolution of the conventional fluorescent microscopes to submicrometer dimensions in both the horizontal and vertical directions. This limitation can be overcome with the aid of photoactivatable fluorophores. Photoactivatable fluorophores switch from a nonemissive state to an emissive one under irradiation at an activation wavelength and then emit light in form of fluorescence upon illumination at an excitation wavelength. Such a concatenation of activation and excitation events translates into the possibility to switch fluorescence on within a defined region of space …
Population Connectivity And Larval Dispersal Of Caribbean Spiny Lobster, Andrew Sebastian Kough
Population Connectivity And Larval Dispersal Of Caribbean Spiny Lobster, Andrew Sebastian Kough
Open Access Dissertations
Studies of movement, fundamental to most life on earth, become complicated in the ocean. Many marine organisms have a complex lifecycle with a larval phase, giving them vast dispersal potential from powerful ocean currents while obscuring direct observation by virtue of their minute size. These research challenges are beginning to be circumvented through biophysical modeling of larval transport. Modeling simulates the potential pathways of larvae, but not fine-scale spatial movements. Despite their small size, larvae actively participate in their dispersive journey, detecting and responding to environmental cues. Orientation behavior has a great impact on a larva’s survival and journey, and …
The Role Of Class I Histone Deacetylases In The Transcriptional Repression Of Estrogen Receptor In Response To Hmapk Signaling, Amy Jayne Plotkin
The Role Of Class I Histone Deacetylases In The Transcriptional Repression Of Estrogen Receptor In Response To Hmapk Signaling, Amy Jayne Plotkin
Open Access Dissertations
Estrogen receptor negative (ER-) breast cancer is more aggressive and associated with both shorter disease-free and overall survival than ER positive breast cancer. Anti-estrogen therapies are not effective in ER- breast cancers, thus identifying mechanisms underlying lack of ER expression in ER- breast cancers is imperative. We have previously demonstrated that hyperactivation of MAPK (hMAPK) downstream of overexpressed EGFR or overexpression/amplification of Her2 represses ER protein and mRNA expression. Abrogation of hMAPK in ER- breast cancer cell lines and primary cultures causes re-expression of ER and restoration of anti-estrogen responses. In investigating the mechanisms underlying hMAPK repression of ER mRNA, …
The Perceived Quality Of Parent-Child Relationships And Dating Violence Experiences Among A Predominantly Heterosexual, Female, And Hispanic-Identified Sample Of Emerging Adults, Amanda M. Cummings
The Perceived Quality Of Parent-Child Relationships And Dating Violence Experiences Among A Predominantly Heterosexual, Female, And Hispanic-Identified Sample Of Emerging Adults, Amanda M. Cummings
Open Access Dissertations
Dating violence has been identified as a serious public health problem among adolescents and emerging adults. Recently, there has been an increase in research focused on identifying risk and protective factors for dating violence across the individual-, relationship-, community-, and societal-levels, yet studies exploring associations between parent-child relationships and dating violence have been lacking. The present study examined the associations between the perceived quality of mother-child relationships and father-child relationships and dating violence victimization and perpetration experiences among a sample of emerging adults. A cross-sectional sample of 454 undergraduate men and women from three universities were assessed at one time …
Interactive Visualization Systems And Sensor-Based Interfaces For Robotics And Medical Data Analysis, Justin W. Stoecker
Interactive Visualization Systems And Sensor-Based Interfaces For Robotics And Medical Data Analysis, Justin W. Stoecker
Open Access Dissertations
Scientific visualization enables researchers to extract understanding from data through visual representations. Interactive visualizations are particularly powerful for exploration of data, but they require that representations can be built and manipulated in real time; this is especially difficult for large or complex data. Advances in human-computer interfaces also provide for new ways to engage with visualizations. This dissertation covers visualization systems that address challenges in visual representation, real-time rendering, and natural interaction. The first domain we consider in this dissertation is robotics. In particular, we focus on the topics of motion and autonomous behavior for robots. We cover three projects …
Gratitude: A Basic Human Emotion For Initiating And Strengthening Interpersonal Relationships, Adam Randall Smith
Gratitude: A Basic Human Emotion For Initiating And Strengthening Interpersonal Relationships, Adam Randall Smith
Open Access Dissertations
Gratitude may function to foster human sociality. Exactly how gratitude performs this putative function, however, is a question that has received little scientific attention. Many recent investigations—primarily concerned with the positive emotional outcomes associated with the experience of gratitude—have overlooked the fundamental mechanistic operations that might produce gratitude. My dissertation addresses this oversight by capitalizing on advancements in the evolutionary psychological study of function and internal regulatory variables. I approach gratitude as a psychological adaptation that functions to coordinate behaviors that initiate and strengthen interpersonal relationships in conjunction with an internal regulatory variable responsible for tracking interpersonal welfare valuation, the …
Analysis Of The Interactions Between Graphene Oxide And Biomolecules And Protein Fibrillation Using Surface Chemistry And Spectroscopy, Shanghao Li
Open Access Dissertations
Graphene oxide (GO), a novel 2−dimensional carbon based nanomaterial, has shown potential applications in biomedical and biological field, including drug and gene delivery, sensing, and bioimaging. However, one critical question needs to be addressed before any actual application: how does GO interact with biological molecules, such as amino acids, peptides, proteins, and biomembranes? In this study, spectroscopy, microscopy, and surface chemistry were applied to fundamentally understand the nature of interactions between these molecules and GO. GO was found to interact with amino acids, peptides, and proteins by fluorescence quenching. The main quenching mechanism between GO and Trp or Tyr was …
Spatial Analysis In Cancer Surveillance, Recinda Loraine Sherman
Spatial Analysis In Cancer Surveillance, Recinda Loraine Sherman
Open Access Dissertations
The burden of cancer is a significant physical, psychological, and financial toll on individuals and populations. Cancer is more common in older populations, so advances in other areas of medicine will inevitably increase the numbers of cancer patients. The development of cancer is prolonged over years which makes it difficult to identify etiologic causes. And the treatment of the disease can also be quite protracted. In the case of advanced or aggressive disease, medical treatment is sometimes reduced to a series of clinical interventions that do little to change the timeline to mortality. Currently, our most effective tool in cancer …
Development Of Helical Chiral Catalysts And Their Applications In Asymmetric Catalysis, Zhili Peng
Development Of Helical Chiral Catalysts And Their Applications In Asymmetric Catalysis, Zhili Peng
Open Access Dissertations
The design and development of novel chiral catalysts for efficient construction of carbon-carbon bonds, especially in an enantioselective manner, stays as a major goal for organic chemists. In the last two decades, various organocatalysts have been developed to solve important problems in asymmetric organic synthesis. Novel chiral catalysts based on previously unexplored 1-azahelicenes have been developed by our group and found great applications in mechanistically unrelated reactions. As part of Ph.D. research projects, the design and development of new helical chiral catalysts based on 11, 12-benzo-1-aza[6]helicene N-oxide and their applications in asymmetric synthesis are presented in this thesis. First, the …
Combinatorial Therapeutic Vaccination Strategies For Hematologic Malignancies In The Early Period Following Experimental Autologous Hematopoietic Stem Cell Transplantation, Robert G. Newman
Open Access Dissertations
Tumor relapse is the primary cause of mortality in patients with hematologic cancers following autologous hematopoietic stem cell transplantation (HSCT). Vaccination early post-HSCT can exploit both the state of lymphopenia and minimal residual disease for the generation of anti-tumor immunity. Here, multiple vaccinations utilizing lymphoma cells engineered to secrete the heat shock protein fusion gp96-Ig within two weeks of T cell replete syngeneic HSCT led to antigen cross-presentation and increased survival of lymphoma bearing mice. To enhance vaccine efficacy, IL-2 was directed to predominantly memory phenotype CD8+ T lymphocytes and natural killer cells via administration bound to anti-IL-2 monoclonal antibody …
Examining Selective Pressures Shaping Retrotransposon Distribution Within Genes, Sara Linker
Examining Selective Pressures Shaping Retrotransposon Distribution Within Genes, Sara Linker
Open Access Dissertations
Retrotransposons (RTs) comprise approximately half of the human genome and contribute to chromatin structure, regulatory motifs, and protein-coding sequences. Since RT insertions can disrupt functional genetic elements as well as introduce new sequence motifs to a region, they have the potential to effect the function of genes that harbour insertions as well as those nearby. A detailed characterization of RT density in genes could help inform predictions of the functional consequence of de novo as well as polymorphic insertions. The research presented here examines the selective pressures that modify the distribution of RTs within genes. It further examines these pressures …
Small Molecule Activation At Nickel And Platinum Centers Containing Bulky Tin And Tempo Radical Ligands, Anjaneyulu Koppaka
Small Molecule Activation At Nickel And Platinum Centers Containing Bulky Tin And Tempo Radical Ligands, Anjaneyulu Koppaka
Open Access Dissertations
Metal complexes have long been recognized as fundamental in activating simple small molecules, which provide low-barrier reaction pathways through binding. One way to make highly reactive metal complexes is by reacting metal precursors with sterically demanding ligands that can create electronic unsaturation on the metal center, where activation can occur. A series of sterically crowded metal complexes have been synthesized by reactions of Pt(COD)2 with bulky tin ligands of the type HSnR3 [ R = But, Ph and C6H2Me3(mesitylene)]. These new complexes were studied for activation of small molecules like H2, CO, C2H2, C2H4, and catalytic H2- D2 exchange reactions …
New Isotopic Methods To Better Understand The Effects Of Ocean Acidification On Net Ecosystem Calcification And Coral Calcification Rates In Bermuda, Alyson M. Venti
New Isotopic Methods To Better Understand The Effects Of Ocean Acidification On Net Ecosystem Calcification And Coral Calcification Rates In Bermuda, Alyson M. Venti
Open Access Dissertations
The health and future of coral and coral reef ecosystems remains threatened by global climate change (GCC) and ocean acidification (OA). There is a need to better understand the current health of coral reef ecosystems, particularly those in threshold environments which will likely be affected first by pressures from GCC and OA. This need can be addressed by answering two critical questions: 1) what are the current net ecosystem calcification (NEC) rates in threshold environments? And 2) what physical, biological or chemical parameter is/are driving these calcification rates? Bermuda, located at the northern threshold to support tropical coral reefs, provides …
Transport Mechanism Of Human Glutamate Transporter Eaat3, Xiaoyu Wang
Transport Mechanism Of Human Glutamate Transporter Eaat3, Xiaoyu Wang
Open Access Dissertations
Excitatory amino acid transporters (EAATs) remove glutamate from synapses in a reuptake process driven by the Na+ gradient to prevent glutamate concentrations from reaching neurotoxic levels. Malfunction of this reuptake mechanism is implicated in stroke. However, methods to treat glutamate transporter malfunction are limited due to the lack of knowledge of the detailed transport mechanism of these transporters under physiological and pathological conditions. To date no crystal structure of human glutamate transporter is available. All crystal structures of glutamate transporter are from the archaeal glutamate transporter homologue Gltph. Inspection of the available Gltph crystal structures in different states gave rise …
Syndrome Specific And Non-Syndrome Specific Predictors Of Developmental Change In Higher Functioning Children With Autism, Kim E. Ono
Open Access Dissertations
There is a wide range of variability in symptoms and comorbid behaviors among individuals with Autism Spectrum Disorder (ASD). Variability is seen both between individuals at a given point in time and in patterns of change within individuals over time. The goals of the current study were to examine (1) initial levels and rates of change in social reciprocity and internalizing and externalizing behavior problems, and (2) the effects of initial temperament, verbal IQ, and symptom severity on mean initial levels and rates of change in social reciprocity and comorbid internalizing and externalizing behaviors. The sample consisted of higher functioning …
Synthesis And Dna-Binding Studies With Two Stericallyfriendly Porphyrin Frameworks, Srijana Ghimire
Synthesis And Dna-Binding Studies With Two Stericallyfriendly Porphyrin Frameworks, Srijana Ghimire
Open Access Dissertations
Peripheral substituents on cationic porphyrins play a significant role during binding with DNA hosts. Possible applications of these systems in photodynamic therapy as well as in anti-bacterial and anti-cancer therapies motivate the binding studies. For characterizing DNA binding motifs different methods are useful including absorption, emission, and circular dichroism spectroscopies, as well as viscometry and X-ray crystallography. With the classic H2 T4 porphyrin, or 5,10,15,20-tetra(N-methylpyridinium-4-yl)porphyrin, the mode of binding varies with the base composition of the DNA host. The porphyrin binds adenine-thymine rich sequences externally whereas intercalation occurs in guanine-cytosine rich sequences. The McMillin group has made some …
High Fidelity Simulations Of Electrokinetic Phenomena In Microfluidic Devices, Qian Li
High Fidelity Simulations Of Electrokinetic Phenomena In Microfluidic Devices, Qian Li
Open Access Dissertations
Electroosmotic flow with electrokinetic effects is the primary method of fluid handling in micro-total analysis systems. Under external applied electric fields, electrokinetic micro-devices allow for innovative functionality in a wide range of microfluidic applications including sample injection, separation, rapid micromixing, and miniaturized chemical and biochemical analysis and detection. This dissertation focuses on simulations of two electrokinetic phenomena, isotachophoresis (ITP) and electrokinetic instability (EKI). A set of coupled governing equations including the incompressible Naiver-Stokes equations, Nernst-Planck transport equations and a charge conservation equation are solved in the simulation. A multiblock in-house solver based on high-order finite difference schemes is developed to …
Metal-Protein Interactions For Proteomics Applications, Keerthi B Jayasundera
Metal-Protein Interactions For Proteomics Applications, Keerthi B Jayasundera
Open Access Dissertations
In the quest of deciphering biological mechanisms, the understanding of proteins and their functions is a vital component. However the complexity of the proteome poses an immense challenge for analytical chemists to explore it comprehensively. Currently mass spectrometry is at the forefront of proteome analysis, providing great capabilities to couple with modern separation techniques for wide applications. One major objective of current proteomic technologies is to identify proteins of interest among high background, in particular from complex biological samples. A variety of techniques have been developed over the years to simplify protein samples, which are broadly classified as enrichment, high …
Energy Efficient Hybrid Computing Systems Using Spin Devices, Mrigank Sharad
Energy Efficient Hybrid Computing Systems Using Spin Devices, Mrigank Sharad
Open Access Dissertations
Emerging spin-devices like magnetic tunnel junctions (MTJ's), spin-valves and domain wall magnets (DWM) have opened new avenues for spin-based logic design. This work explored potential computing applications which can exploit such devices for higher energy-efficiency and performance. The proposed applications involve hybrid design schemes, where charge-based devices supplement the spin-devices, to gain large benefits at the system level. As an example, lateral spin valves (LSV) involve switching of nanomagnets using spin-polarized current injection through a metallic channel such as Cu. Such spin-torque based devices possess several interesting properties that can be exploited for ultra-low power computation. Analog characteristic of spin …
Human And Canine Personality Assessment Instruments To Predict Successful Adoptions With Shelter Dogs, Sheryl Lynn Walker
Human And Canine Personality Assessment Instruments To Predict Successful Adoptions With Shelter Dogs, Sheryl Lynn Walker
Open Access Dissertations
Animal shelters are often over-crowded with animals, and efforts to match potential adopters with shelter dogs, to improve the quality of adoptions, are increasing. However, a lack of evidence-based practices makes matching difficult. This research was conducted to investigate the role of dog and human personality, using questionnaire-based measurements, on adoption success in two Indiana shelters, Clinton County Humane Society and the Humane Society of Indianapolis. Ultimately, the aim of this project was to assess dog personality, human personality, and satisfaction, to evaluate adoption success in shelter dogs.^ The present thesis contains three studies exploring dog and human personality traits, …
Novel Energy Efficient Electrohydraulic Steer-By-Wire Technology, Naseem Daher
Novel Energy Efficient Electrohydraulic Steer-By-Wire Technology, Naseem Daher
Open Access Dissertations
Improving energy efficiency, productivity, safety, and comfort of mobile machines is of utmost priority to original equipment manufacturers, suppliers, and consumers given the escalating fuel prices and increased awareness to the environment and workplace hazards. A major breakthrough in the realm of high power motion control is pump displacement controlled (DC) actuation, which does away with hydraulic valves for motion control and uses a variable displacement pump as both a flow source and final control element, thus eliminating throttling losses associated with hydraulic control valves. This work deals with researching and implementing DC technology for realizing the steering function of …
Interfacial And Spectroscopic Studies Of Biomacromolecules And Cdse(Zns) Quantum Dots, Nicholas Franklin Crawford
Interfacial And Spectroscopic Studies Of Biomacromolecules And Cdse(Zns) Quantum Dots, Nicholas Franklin Crawford
Open Access Dissertations
Understanding of biomacromolecule interactions at the molecular level raises certain difficulties however with the Langmuir monolayer technique, interfacial interactions in two dimensions can be accurately measured to access and characterize physical, electrostatic and photophysical properties. Macromolecules such as serum albumin which comprises 50% of human blood plasma protein content is one focus of research in this thesis for its unique involvement in many biological functions. The important nature of this class of protein demands that it be studied in detail while modifying the experimental conditions in two dimensions to observe it in all types of environments. While different from bulk …
Immigrant Diversity And Host Country Acculturation In Leader-Member Dyads, Monica M. Sharif
Immigrant Diversity And Host Country Acculturation In Leader-Member Dyads, Monica M. Sharif
Open Access Dissertations
Leadership scholars have investigated the importance of leader-member dyads for several years. However, research has largely omitted the consideration of immigrant diversity with regards to leader-member relationships and outcomes in the organization. The US has experienced an influx of talented immigrants that are joining the workforce and, therefore, important to today’s economy. In a sample of two organizations, one based in South America, and another headquartered in Miami, Florida, I propose two models. One model examines the role of country of origin incongruence and LMX and the other examines the role of host country acculturation and LMX. Immigrants come from …
Mechanisms Of Mitochondrial Regulation And Ischemic Neuroprotection By The Pkcε Pathway, Kahlilia Morris-Blanco
Mechanisms Of Mitochondrial Regulation And Ischemic Neuroprotection By The Pkcε Pathway, Kahlilia Morris-Blanco
Open Access Dissertations
Mitochondrial dysfunction following cerebral ischemia is a major contributor to tissue injury and neurodegeneration. Therefore, investigating the pathways that lead to mitochondrial protection following cerebral ischemic injury represents a major strategy in the development of therapeutic approaches. The PKCepsilon enzyme has been shown to translocate to the mitochondria where it is involved in providing global mitochondrial protection against ischemic insults. The goal of this study was to better understand the downstream pathways that drive PKCepsilon-mediated mitochondrial neuroprotection which may facilitate therapeutic approaches to cerebral ischemic injury. Initial experiments performed in vitro revealed that PKCepsilon functionally cooperates with the transcriptional regulator …
Cognitive Mechanisms Of Anger Regulation: The Role Of Executive Function, Katherine G. Denny
Cognitive Mechanisms Of Anger Regulation: The Role Of Executive Function, Katherine G. Denny
Open Access Dissertations
Anger is a prevalent and powerful emotion, arising when another person has thwarted a self-relevant goal or event, and increased effort is required for goal achievement. While the majority of research indicates that anger is an affective precursor to aggression, recent research implies that anger may also have beneficial consequences – at least for some people in some situations. With the negative cost of anger often being severe, and yet the potential that anger utilization can lead to enhanced performance, it is important to understand individual differences in the ability to regulate and utilize anger. To explore this area of …
Living In Limb/O: Reading Dismemberment And Orphanhood In Contemporary Spanish Caribbean Literature, Sara Jill Gusky
Living In Limb/O: Reading Dismemberment And Orphanhood In Contemporary Spanish Caribbean Literature, Sara Jill Gusky
Open Access Dissertations
Seeking alternative metaphors for understanding the Caribbean experience as it is illustrated in contemporary Spanish Caribbean novels, this dissertation explores broken bodyscapes in order to approach the organizing metaphor of dismemberment. I establish a metaphorical connection between the body with missing limbs and the family tree with missing limbs in order to map the space of limb/o in which both allegorical and literal orphans are depicted in the following novels: La casa de la laguna (1997) by Rosario Ferré, Nuestra señora de la noche (2006) and Sirena Selena vestida de pena (2000) by Mayra Santos-Febres, El reino de este mundo …
The Effect Of A Culturally-Informed Therapy For Schizophrenia On Caregiver Burden, Perceptions Of Interdependence And Self-Conscious Emotions, Giulia Caitlin Suro
The Effect Of A Culturally-Informed Therapy For Schizophrenia On Caregiver Burden, Perceptions Of Interdependence And Self-Conscious Emotions, Giulia Caitlin Suro
Open Access Dissertations
Research has demonstrated that taking care of a patient with schizophrenia has serious mental health costs to caregivers that include high levels of burden and poorer overall mental health. Thus, it is necessary to pinpoint predictors of caregiver distress and develop strategies to reduce it. We have developed a family focused, culturally-informed treatment for schizophrenia (CIT-S). We examined this intervention and its ability to decrease the maladaptive behaviors, beliefs, and values that we believe contribute to caregiver distress. CIT-S was compared against a three-session psychoeducation condition in a sample of 113 caregivers of patients with schizophrenia. Specifically, we hypothesized that …
Queer Transnationality: Narrative, Theatre, And Performance Across Temporal, Spatial, And Social Geographies, Alexandra Gonzenbach Perkins
Queer Transnationality: Narrative, Theatre, And Performance Across Temporal, Spatial, And Social Geographies, Alexandra Gonzenbach Perkins
Open Access Dissertations
This study investigates the ways narrative, theatre, and performance art negotiate identities across Cuba, the Dominican Republic, Puerto Rico, the United States, and larger global contexts. I research how physical, social, and temporal spaces transform enactments and receptions of text and performance. Particularly, I investigate the effects of transnationality in works that represent queer subjectivities. I intervene in Queer Studies, expanding the notion of queer beyond sexuality to include nationality, race, and class. While identity politics emerges around fixed categories of identity, I argue that representations of subjectivity in transnational networks of production dissociate politicized identification from the rigid geographic …