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Open Access Dissertations

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Obesity And Colorectal Cancer And The Knowledge, Attitudes, Beliefs And Behaviors Related To Colorectal Cancer Prevention Among Non-Hispanic Black Women In Rhode Island, Kathleen Mary Cullinen Jan 2005

Obesity And Colorectal Cancer And The Knowledge, Attitudes, Beliefs And Behaviors Related To Colorectal Cancer Prevention Among Non-Hispanic Black Women In Rhode Island, Kathleen Mary Cullinen

Open Access Dissertations

Obesity is an established risk factor for colorectal cancer (CRC). Numerous studies have linked diets low in fruits and vegetables and decreased physical activity, risk factors for obesity, with increased cancer risk. The purpose of this study was to evaluate the effect of obesity on CRC mortality; the behavioral, socioeconomic and demographic predictors of obesity; and the knowledge attitudes, beliefs and behaviors of a Rhode Island population identified at high risk for both obesity and CRC. The first study tested a model hypothesis that a diet low in fruits and vegetables, decreased physical activity, and obesity as defined by a …

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Applicability Of The Transtheoretical Model In Weight Management In An Adolescent Population In Taiwan, Yating Yeh Jan 2005

Applicability Of The Transtheoretical Model In Weight Management In An Adolescent Population In Taiwan, Yating Yeh

Open Access Dissertations

Westernized dietary preferences and life-styles are thought to have a significant impact on the increasing prevalence of obesity in Asian populations. The increased numbers of overweight and obese adolescents is becoming an emerging health issue in Asia. Meanwhile, previous studies have indicated that adolescents ' self-perceived weight is poorly related to the medical definition of overweight, and considerable numbers of clinically normal-weight persons are trying to or wish to control weight. Due to the considerable prevalence of desire for weight control among adolescents who are not overweight /obese, it is important for researchers to look at the issue of weight …

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An Assessment Of The Habitat Quality And Nutritional Intake Of North Atlantic Right Whales In Cape Cod Bay, Amy Susanna Delorenzo Jan 2005

An Assessment Of The Habitat Quality And Nutritional Intake Of North Atlantic Right Whales In Cape Cod Bay, Amy Susanna Delorenzo

Open Access Dissertations

Cape Cod Bay is the only known winter feeding ground of the North Atlantic right whale, a seriously endangered species, and is thus a critical habitat for right whales. As with most coastal ecosystems, Cape Cod Bay is a dynamic system with a high degree of variability in both physical and biological parameters. However, the extent of these fluctuations and their effect on the suitability of the bay as a right whale feeding ground is not known. This study was conducted to address variability in the biological and physical environment of the bay on different temporal and spatial scales and …

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Recombinant Antimicrobials For Feed Based Delivery In Aquaculture, Tarquin Dorrington Jan 2005

Recombinant Antimicrobials For Feed Based Delivery In Aquaculture, Tarquin Dorrington

Open Access Dissertations

Two antimicrobial peptides of marine origin, tachyplesin from the horseshoe crab (Tachyplesus tridentatus) and pleurocidin from the winter flounder (Pseudopleuronectes americanus), were evaluated for potential use in feed-based delivery in oyster aquaculture. Tachyplesin, a beta sheet structure was more effective against a range of gram-positive and gram-negative pathogens, as well as against the protozoan oyster parasite Perkinsus marinus. In addition, tachyplesin's structure made it more resistant to the effects of proteases from the oyster and the parasite. The two peptides also had different effects on the host bacteria of the oyster digestive system, with tachyplesin selectively killing more gram-negatives and …

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Energetic Constraints On Diapause In Calanus Finmarchicus: Implications For Population Dynamics In The Gulf Of Maine, Whitley Joseph Saumweber Jan 2005

Energetic Constraints On Diapause In Calanus Finmarchicus: Implications For Population Dynamics In The Gulf Of Maine, Whitley Joseph Saumweber

Open Access Dissertations

Deep basins in the Gulf of Maine act as refuge for a large population of resting Calanus finmarchicus during the summer and fall. Dynamics of this population are not well understood, however, and loss terms from advection and starvation may be large. A three part study was therefore undertaken to determine potential diapause duration in C. finmarchicus , monitor changes in the summer-fall population, and to define the relative importance of energetic limitation as a control on C. finmarchicus population dynamics. Six standard stations were sampled in and around the Gulf of Maine on a series of four research cruises …

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Community Impacts Of Coastal Tourism In The Dominican Republic, Yolanda M. Leon Jan 2004

Community Impacts Of Coastal Tourism In The Dominican Republic, Yolanda M. Leon

Open Access Dissertations

The present work presents the results of survey research conducted in 23 coastal communities of the Dominican Republic to evaluate the impacts of tourism and also the evaluation of a particular co-management system of a tourism-related activity (whale watching in Samaná Bay). Major findings include that tourism is having a positive impact on rural livelihoods as measured by increased household income, and higher levels of job satisfaction (and in the case of female-headed households, also improved material well-being). We also evidenced strong local support for the tourism industry caused by wide agreement on perceived tourism benefits; however, residents are also …

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Ecomorphology Of The Temperate Scleractinian Astrangia Poculata: Coral-Macroalgal Interactions In Narragansett Bay, Sean Patrick Grace Jan 2004

Ecomorphology Of The Temperate Scleractinian Astrangia Poculata: Coral-Macroalgal Interactions In Narragansett Bay, Sean Patrick Grace

Open Access Dissertations

Corals and macroalgae often co-occur on temperate reefs, where macroalgae provide a majority of the bio-complexity and small scleractinian corals occur in low abundance. Macroalgae typically outcompete adjacent organisms for resources such as space, light, and nutrients, and one mechanism for this competitive success is abrasion. This study explores factors that may influence the interactions between corals and macroalgae, in particular macroalgal abrasion, on a temperate reef in Narragansett Bay, Rhode Island. Chapter One examines how exposure, depth and orientation influence the abundance and morphology of the coral Astrangia poculata, as well as the percent cover of macroalgae and other …

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Culture Of Summer Flounder Paralichthys Dentatus: Environmental And Endocrine Synchronization Of Growth And Settlement In Larvae And Identification Of Stressors In Juveniles, Steven Gavlik Jan 2004

Culture Of Summer Flounder Paralichthys Dentatus: Environmental And Endocrine Synchronization Of Growth And Settlement In Larvae And Identification Of Stressors In Juveniles, Steven Gavlik

Open Access Dissertations

This dissertation addresses the variability in settlement behavior, growth and development during metamorphosis of summer flounder. There were three objectives: (1) Expand basic knowledge of hormonal and environmental factors that regulate timing of metamorphosis, (2) develop hormonal and environmental techniques that promote synchronization of metamorphic growth and settling behavior in order to eventually reduce cannibalism in juveniles, and (3) identify stressful culture practices and evaluate their long-term effects on growth in juvenile flounder. The first and second objectives were accomplished through manipulations of cortisol and thyroxine and of rearing salinity. Endogenous whole-body cortisol concentrations increased through the late larval stages, …

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Regulation Of Cra, A Regulatory Gene Of Glycolytic And Gluconeogenic Pathways In Salmonella Enterica Serovar Typhimurium, James H. Allen Jan 2004

Regulation Of Cra, A Regulatory Gene Of Glycolytic And Gluconeogenic Pathways In Salmonella Enterica Serovar Typhimurium, James H. Allen

Open Access Dissertations

The Cra protein is a global regulator of carbon and energy metabolism for glycolysis and gluconeogenesis. The live oral vaccine candidate, Salmonella typhimurium SR-11 Fad, is unable to utilize gluconeogenic substrates as sole carbon sources and is avirulent and protective in BALB/c mice. Furthemore, the era gene is interrupted in this strain of Salmonella. The cra gene was suspected to be regulated by another global regulator, acetyl phosphate. To further investigate the regulation of the cra gene and to determine if gluconeogenesis is linked to virulence, mutations in the gluconeogenic genes fbp (fructose-1,6-bisphosphatase), maeB (NADP-dependent malic enzyme), and sfcA …

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Development And Validation Of The Proactive Health Lifestyle Measures, Norma Jean Perez Jan 2004

Development And Validation Of The Proactive Health Lifestyle Measures, Norma Jean Perez

Open Access Dissertations

This research project targets the three leading causes of death in the U.S., cardiovascular disease, cancer, and stroke through the successful development and validation of six culturally sensitive Proactive Healthy Lifestyle measures (Stage of Change, Decisional Balance, Self-Efficacy, Skills, Relapse, and Processes of Change) targeting diet, exercise, and smoking. 574 college students were recruited and completed self-report surveys. Data from 418 non-Hispanics was used to develop (N = 209) and validate (N = 209) each of the measures. Data from 155 Hispanics was used to further successfully validate the measures, and explore differences in this group with the non-Hispanic group. …

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A Meta-Analytic Examination Of Decisional Balance Across Stage Transitions: A Cross-Sectional Analysis And Cross-Sequential Cross-Validation, Kara L. Hall Jan 2004

A Meta-Analytic Examination Of Decisional Balance Across Stage Transitions: A Cross-Sectional Analysis And Cross-Sequential Cross-Validation, Kara L. Hall

Open Access Dissertations

Interventions to decrease unhealthy behaviors and to increase healthy behaviors are crucial for health promotion and disease prevention. Computerized tailored interventions provide a promising approach for creating positive health behavior change. The Transtheoretical Model (TTM) delineates a way to conceptualize behavior change, provides the foundation for developing assessments of an individual's readiness to change, and is utilized in tailoring interventions for actualizing behavior change. To produce optimally tailored interventions: (1) theory that guides interventions needs to be comprehensively tested; and (2) empirical data that drive the tailored interventions needs to be systematically generated. This program of research tests theoretical assumptions …

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Metabolic Routing Of Macronutrients In Migratory Songbirds: Effects Of Diet Quality And Macronutrient Composition Revealed Using Stable Isotopes, David William Podlesak Jan 2004

Metabolic Routing Of Macronutrients In Migratory Songbirds: Effects Of Diet Quality And Macronutrient Composition Revealed Using Stable Isotopes, David William Podlesak

Open Access Dissertations

Animals metabolically route specific macronutrients from their diet to build and maintain tissue and researchers generally assume that the isotopic composition of an animal's tissues resembles the isotopic composition of their diet. However, animals regularly change diets and they may route dietary macronutrients differently to specific tissues. Thus, I studied metabolic routing of dietary macronutrients into animal tissue because it is fundamentally important for understanding the nutritional ecology of wild vertebrates and because such knowledge is essential for accurate interpretation of stable isotope patterns in consumers and consumed. Songbirds that routinely switch their diets during migration provide a particularly interesting …

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Parents' Descriptions Of Ideal Home Nursing Care For Their Technology Dependent Child, Themselves, And Their Families, Michele A. Mendes Jan 2004

Parents' Descriptions Of Ideal Home Nursing Care For Their Technology Dependent Child, Themselves, And Their Families, Michele A. Mendes

Open Access Dissertations

Home care for technology dependent children is one of the fastest growing segments of the U.S. health care industry, but the nursing literature lacks clear directions to guide home care nurses in planning a family-centered practice. The purpose of this study is to address this gap in the literature by eliciting descriptions of ideal home nursing care for technology dependent children from the perspective of their parents, and to compile a comprehensive list of helpful and supportive nursing interventions. A qualitative, descriptive design was used to examine the following research questions: (1) How do parents of technology dependent children describe …

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The Factors That Influence Middle-Level Teachers To Incorporate Community Service-Learning Into The Curriculum, Andrea J. Hainey-Turcotte Jan 2004

The Factors That Influence Middle-Level Teachers To Incorporate Community Service-Learning Into The Curriculum, Andrea J. Hainey-Turcotte

Open Access Dissertations

This study examined the factors that influence middle level educators to incorporate community service-learning into the curriculum. Eleven participants, each a teacher of middle level students, were interviewed to determine the extent to which they were influenced by intended outcomes for students, the community, and themselves. Findings suggest that participants were influenced to include community service-learning in the curriculum by anticipated benefits to students and community members. Participants hoped that by engaging in community service-learning activities, their students would become better scholars, leaders, citizens, and/or people. It would seem that participants were also driven to the implementation of community service-learning …

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The Role Of Glycolytic Substrates In The Initiation And Maintenance Phases Of Colonization Of The Mouse Large Intestine By Escherichia Coli Mg1655 And Escherichia Coli Edl933, Regina L. Miranda Jan 2004

The Role Of Glycolytic Substrates In The Initiation And Maintenance Phases Of Colonization Of The Mouse Large Intestine By Escherichia Coli Mg1655 And Escherichia Coli Edl933, Regina L. Miranda

Open Access Dissertations

Escherichia coli is a gram-negative motile rod, and is one of the most common bacterial strains isolated from the human intestinal tract (1,2). E. coli K-12, strain MG1655, is a non-pathogenic laboratory strain of E. coli whose genome has been fully sequenced (1). E. coli O157:H7, strain EDL933, a shigatoxin-producing E. coli (STEC), is a human pathogen, whose genome has also been fully sequenced (34). E. coli EDL933 was first isolated in August of 1982 from patients in the United States suffering from an outbreak of bloody diarrhea, which was traced to contaminated hamburgers (8,9,10,11). E. coli O157:H7 infections are …

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Biological Control Of The Lily Leaf Beetle, Lilioceris Lilii In North America, Marion Secrest Gold Jan 2003

Biological Control Of The Lily Leaf Beetle, Lilioceris Lilii In North America, Marion Secrest Gold

Open Access Dissertations

The lily leaf beetle, Lilioceris lilii (Scopoli), first officially reported in the U.S. in 1992, has become a serious pest of native and cultivated lilies throughout its North American distribution. With no effective North American natural enemies, this exotic insect poses a potential economic threat to horticulturists and an environmental threat to native lilies. This study presents the results of an ongoing classical biological control program initiated against L. lilii in North America. Based on its Eurasian geographic distribution, L. lilii seems capable of spreading throughout much of North America. In Europe, the lily leaf beetle is well known but …

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Longitudinal Study Of The Acquisition Of Locomotion, Motor Activity, And Infant Development, Janette Baird Jan 2003

Longitudinal Study Of The Acquisition Of Locomotion, Motor Activity, And Infant Development, Janette Baird

Open Access Dissertations

This study explored the relationship between the age of development of 5 locomotor behaviors, the level of infant activity for the locomotor behaviors, and the performance of the sample of infants on standardized indices of infant development (Fagan Test of Infant Intelligence, FTII; Bayley Scales of Infant Development, BSID-II). A total of 157 infants were assessed at 6, 9, and 12 months for their activity levels on the 5 locomotor behaviors of interest, and the age at which each locomotor behavior occurred was obtained through records maintained by each infants' caregiver. The results of the regression path analysis indicates that …

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Ferulic Acid And Peroxidase Infusion Into Disparate Vegetables And Its Effect On Texture, Karen Rose Conca Jan 2003

Ferulic Acid And Peroxidase Infusion Into Disparate Vegetables And Its Effect On Texture, Karen Rose Conca

Open Access Dissertations

The texture quality of canned fruits and vegetables can be severely degraded or softened due to thermal processing. Increased ferulic acid cross-linking and cell-cell adhesion has been associated with increased firmness of texture in fruits and vegetables. In order to test such a theory for maintaining firmness and cross-linkages of ferulic acid after thermal processing, vegetables were pre-treated/infused with peroxidase enzyme and ferulic acid. Carrots, beets and Chinese water chestnuts were chosen for their extreme differences in texture. Various methods for measuring texture were tested. Flat head compression was selected as the most sensitive method of assessing texture. Various infusion …

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Micropropagation Of Carnivorous Plants, Kenneth Stephen Uhnak Jan 2003

Micropropagation Of Carnivorous Plants, Kenneth Stephen Uhnak

Open Access Dissertations

A simple, efficient system for seed surface disinfestation and in vitro germination was developed for the carnivorous pitcher plants Darlingtonia californica and Sarracenia leucophylla. Of the disinfectants tested, hydrogen peroxide or 10% Clorox® were most effective for disinfesting seeds of D. californica, while concentrated sulfuric acid worked best for S. leucophylla. Differences in the effectiveness of sterilants were associated by differences in seed coat morphology. Seeds of D. californica imbibed at 4-7°C in sterile deionized water with surfactant and gibberellin germinated earlier than seeds without exposure to gibberellin. Unimbibed seeds of S. leucophylla germinated …

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Drug Benefit Plans For Elderly Under Managed Care And Utilization Of Lipid Lowering Agents, Susan M. Abughosh Jan 2003

Drug Benefit Plans For Elderly Under Managed Care And Utilization Of Lipid Lowering Agents, Susan M. Abughosh

Open Access Dissertations

Many health maintenance organizations (HMOs) have implemented programs providing varying degrees of annual drug coverage for Medicare beneficiaries enrolled in their plans. Older adult plan members in an HMO operating in central Massachusetts were able to choose among 3 drug benefit options starting January 1, 1994: full coverage for prescription drugs, a maximum of $1000/year in coverage, or no drug coverage. As such, cost containment policies have been shown to affect prescription drug use and other types of health service utilization. The unintended effects of this policy are important to consider. This research investigated the effects of type of drug …

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Assessing The Impact Of Habitat And Stock Enhancement For The American Lobster (Homarus Americanus), In Narragansett Bay, Rhode Island, Kathleen Monan Castro Jan 2003

Assessing The Impact Of Habitat And Stock Enhancement For The American Lobster (Homarus Americanus), In Narragansett Bay, Rhode Island, Kathleen Monan Castro

Open Access Dissertations

Habitat enhancement and stock enhancement methods were examined as to their effect on abundance of the lobster, Homarus americanus, in Dutch Harbor in Narragansett Bay, Rhode Island. Six experimental artificial reefs for lobster were established in Dutch Harbor in February 1997. The reefs were constructed identically consisting of two portions made up of cobble and boulders. The area was monitored for 6 months before placement and five years after placement using a combination of visual surveys by SCUBA divers, trap sampling, a tag-recapture program and airlift sampling for young of the year. Two areas were used as comparison sites, a …

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The Effects Of Distress And Depression On Exercise Behaviors Of Type 2 Diabetics: Applying The Transtheoretical Model, Kelly Lynn Fisher Jan 2003

The Effects Of Distress And Depression On Exercise Behaviors Of Type 2 Diabetics: Applying The Transtheoretical Model, Kelly Lynn Fisher

Open Access Dissertations

Adherence to self-management regimens among individuals with type 2 diabetes is a major issue. The purpose of this study was to examine the relationship of adjustment distress and depression, in relation to the Transtheoretical Model constructs of stage of change, processes of change, decisional balance, and self-efficacy for exercise behavior. A convenience sample of 125 type 2 diabetics between the ages of 33 to 86 (mean age of 62.3), were from a diabetes outpatient education center. Data was collected by mailing the following instruments: demographic survey, Short Geriatric Depression Scale, Problem Areas In Diabetes Survey, and Exercise versions of Stages …

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The Influence Of Cognitive Reserve On Neuropsychological Functioning After Coronary Artery Bypass Grafting, Susan Anne Legendre Jan 2003

The Influence Of Cognitive Reserve On Neuropsychological Functioning After Coronary Artery Bypass Grafting, Susan Anne Legendre

Open Access Dissertations

Neuropsychological functioning is negatively affected in many individuals who undergo coronary artery bypass grafting (CABG). However, the degree and consistency of this impairment is variable. Research in the fields of Alzheimer's disease and HIV suggests that the impact of various assaults to the central nervous system may be mediated through some form of cognitive reserve. The concept of cognitive reserve (CR) is based on the hypothesis that an individual's genetic make-up or experiences acquired throughout life, such as educational and occupational attainment, provide a reserve which allows an individual to sustain greater cerebral injury before clinical presentation of dysfunction. The …

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The Nutritional Ecology Of Songbirds: How Food Quality, Diet Preferences, And Food Limitation Influence Nutrient Storage And Use During Migration, Barbara Jean Pierce Jan 2003

The Nutritional Ecology Of Songbirds: How Food Quality, Diet Preferences, And Food Limitation Influence Nutrient Storage And Use During Migration, Barbara Jean Pierce

Open Access Dissertations

Birds during migration alternate between periods of fasting during active flight and then intense re-feeding at stopover sites as they prepare for their next flight. At stopover sites, birds must select foods that satisfy their elevated nutrient and energy requirements even though food quantity and quality at these sites is often unpredictable. The primary goals of my research were to (1) examine the effects of food quality, food quantity, and digestive physiology on the dynamics of nutrient reserves in a small migratory songbird, (2) determine how nutrient preferences and diet influence the composition of nutrient reserves in a small migratory …

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The Regulation And Function Of Inositol Phosphorylceramide Synthase In The Yeast Saccharomyces Cerevisiae, Angeleah Arlene Browdy Jan 2002

The Regulation And Function Of Inositol Phosphorylceramide Synthase In The Yeast Saccharomyces Cerevisiae, Angeleah Arlene Browdy

Open Access Dissertations

IPC Synthase has been shown to be an essential membrane bound enzyme in yeast that catalyzes the transfer of PI to ceramide to yield the primary sphingolipid found in yeast, Inositolphosphorylceramide. A biochemical approach was taken to investigate the biological factors that regulate IC synthase activity in order to better understand the sphingolipid synthesis. A highly purified IPC synthase was purified over 1000-fold from the yeast Saccharomyces cerevisiae by a procedure that included Triton X-100 solubilization, as well as a series of ion exchange and hydrophobic column chromatography steps. The highly purified enzyme fraction contained three major bands at 45, …

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An Analysis Of The Predictive Power Of The Transtheoretical Model Of Behavior Change Applied To Dietary Fat Intake, Julie A. Wright Jan 2002

An Analysis Of The Predictive Power Of The Transtheoretical Model Of Behavior Change Applied To Dietary Fat Intake, Julie A. Wright

Open Access Dissertations

The present research was designed to advance the theory and science of health behavior change by evaluating one of the most commonly used behavior change theories, the Transtheoretical Model (TTM). Two criteria were selected for evaluation, empirical adequacy (predictive power) and generalizability of the TTM to dietary fat intake. Predictive power was evaluated by testing evidence-based a priori predictions. Generalizability was evaluated by testing a priori predictions founded on smoking data and tested on diet data. Longitudinal data from a large scale randomized control trial on adults recruited from primary care practices were used to test a priori predictions of …

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Genetic Transformation Of Bentgrasses For Fungal Disease Resistance, Yuexia Wang Jan 2002

Genetic Transformation Of Bentgrasses For Fungal Disease Resistance, Yuexia Wang

Open Access Dissertations

Genetic transformation of cultivated plants is a new tool that could be used to improve plants for higher yield, better quality, and enhanced stress tolerance. To establish a stable and efficient biolistic-mediated genetic transformation system for bentgrass trait improvement, this research focused on: (1) comparing growth media for embryogenic callus induction and plantlet regeneration; (2) transferring bar, chitinase, glucanase and barnase genes into embryogenic calli of creeping and velvet bentgrasses, and regenerating transgenic plants from the transformed calli; (3) evaluating the transgenic plants by using herbicide glufosinate, polymerase chain reaction (PCR), Southern blot and northern blot hybridization assays; and (4) …

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Market Information And Fisheries Management: Improving Efficiency In Private Property Right Management Systems, Eyjolfur Gudmundsson Jan 2002

Market Information And Fisheries Management: Improving Efficiency In Private Property Right Management Systems, Eyjolfur Gudmundsson

Open Access Dissertations

Fisheries economics concentrates on solving the problem of economic overexploitation in fisheries. The field took its first steps, as a specialized study area within economics, in the early 1950s. The approach since then, for solving the problem of overexploitation, has mostly been one of neo-classical economics, with emphasis on the production side of the problem. A potential solution to the overexploitation problem is the privatization of fisheries in order to maximize long-term economic benefits. Recent studies have shown that prices and certain product characteristics are an important factor for determining the optimal management of fisheries. This study tries to establish …

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An Exploratory Study Of The Effects Of A Training Program On The Ability To Prepare A Breakfast Meal By Clients With Mild Alzheimer's Disease, Alicia J. Curtin Jan 2002

An Exploratory Study Of The Effects Of A Training Program On The Ability To Prepare A Breakfast Meal By Clients With Mild Alzheimer's Disease, Alicia J. Curtin

Open Access Dissertations

Alzheimer's disease (AD) is characterized by the insidious onset of an array of cognitive deficits that progress over time and lead to changes in an individual's ability to even perform simple tasks, such as preparing meals. Recently, researchers have demonstrated that clients with mild AD have preserved procedural memory function despite significant cognitive deficits. Procedural memory is an automatic, unconscious process, requiring practice and repetition to activate the particular structures involved in learning tasks. Skills training is a strategy using repetition and practice to enhance the client's ability to perform a certain task. An exploratory design using direct observation and …

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β-Glucuronidase Assay For The Detection Of Escherichia Coli In Foods, Seref Tagi Jan 2001

β-Glucuronidase Assay For The Detection Of Escherichia Coli In Foods, Seref Tagi

Open Access Dissertations

Enzyme assays were developed for the measurement of β-D-glucuronide (GUS) as a rapid detection method for E. coli in food. A fluorescent assay utilizing 4-methylumbelliferyl-β-D-glucuronide as substrate was optimized and tested with growth cultures of E. coli. A relationship between E. coli and the level of GUS activity was established. A chemiluminescent assay using a 1,2-dioxetene derivative as substrate was compared to the fluorescent assay, and was found to be 10x more sensitive. Anti-E. coli GUS antibodies were covalently immobilized on magnetic beads and used in a 30 min immunocapture method. GUS was recovered from E. coli grown at 37°C …

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