Digital Commons Network^™

Induction Of Zika And Dengue Virus-Specific Cd8+ T Cells By Means Of Dna Vaccine, Natalie Jachym

Master's Theses

Zika virus (ZIKV) and Dengue virus (DENV) are flaviviruses that circulate in the same endemic regions and can co-infect the same individual. While primary infection by DENV leads to life-long immunity to the infecting serotype, it does not provide immunity to other serotypes. Moreover, ZIKV and DENV antibodies can be cross-reactive and potentially enhance infection of the other virus through a process known as antibody-dependent enhancement (ADE). In this mechanism, antibodies weakly bind the infecting virus and direct virions to immunoglobulin Fc receptors on macrophages. This promotes viral uptake and infection rather than neutralize the virus. Due to this mechanism, …

Reducing Reperfusion Injury In An Adult Rat Stroke Model With St-115 Treatment, Morgan Rae Lenz

Master's Theses

The restoration of blood flow to ischemic cerebral tissue can result in reperfusion injury, and blocking reperfusion injury can significantly aid in recovery of ischemic tissue. Bradykinin has been shown to exhibit protective effects, but does not reach protective levels during reperfusion due to inactivation by aminopeptidase P2 (APP2). Therefore, inhibiting APP2 during reperfusion is a potential therapeutic strategy to aid in the reduction of reperfusion injury, leading to the development of ST-115, an APP2 inhibitor. It was hypothesized that administration of ST-115 during ischemic stroke, and just prior to reperfusion, will result in reduced neurological injury and increased functional …

Educational Debt: Educational Loans And The Family, Keyla Navarrete

Master's Theses

Student debt is a well-documented topic in sociological literature. It is well known that there is a student loan crisis in the United States. However, kinship or familial ties in educational debt is not as studied as individual student loans. The student debt crisis seems to reach a new catastrophic level as years pass. Yet, not much research exists that looks at external sources of financing for students such as parents, grandparents, or other familial ties. This study contributes to the literature of student debt by analyzing debt patterns across those that take out loans for themselves, their spouse, or …

Dna Methylation Patterns Of Repetitive Elements On Hc21, Navy Neang

Master's Theses

Cancer is one of the most prominent diseases resulting in death, thus the need for early detection remains essential to its treatments. It is usually associated with DNA methylation changes during tumorigenesis. While many studies focus on euchromatin, some studies have looked at the methylation changes in heterochromatin, since the methylation changes of repetitive elements is common and they contribute to karyotypic instability leading to enhanced chances for more mutations in tumor progression. Using bisulfite PCR and HC21 as a model, this study examines the DNA methylation changes of three repetitive elements in prostate cancer, NBL2, satellite I subfamilies, and …

The (Mis)Education About Enslavement: The Portrayals Of Enslavement In 3rd Through 5th Grade Social Science Curriculum, Sondrea Singleton

Master's Theses

It is no secret that America's history is one that reflects participation in chattel slavery in 1619. What is untold is the ways in which that part of America's past is reflected in curriculum. The question guiding this research is, "How is enslavement portrayed in 3rd through 5th grade social science curriculum?" to better understand the portrayals, representations, and messages communicated about enslavement to young learners. This research is a qualitative exploration of the ways in which enslavement is portrayed in 3rd through 5th grade social science curriculum by two of the most widely used publishing companies, McGraw Hill and …

Emotion Reactivity And Its Relation To Daily Affect And Sleep Experiences Among First Year College Students, Elizabeth Marie Rea

Master's Theses

Individuals with high emotion reactivity may be at increased likelihood to experience emotions more intensely, frequently, and for longer periods of time than those with low emotion reactivity. This might lead to more extreme fluctuations in their daily experience of emotion. Additionally, those high in emotion reactivity may be at risk for poor sleep. The transition to college has been shown to be a difficult time for some students, and potentially contribute to these emotional fluctuations and shorter sleep duration. However, students high in emotion reactivity might be more likely to experience the negative effects of this transition than those …

A Chronic Route?: Examining The Path Between Community Violence Exposure, Posttraumatic Stress & Juvenile Delinquency In Low-Income, Urban, African-American Youth, Kaleigh Valencia Wilkins

Master's Theses

Background: Exposure to community violence (ECV; direct victimization and witnessing) can predict negative outcomes for youth such as posttraumatic stress (PTSS) and juvenile delinquent behavior (JDB). Psychosocial reactions to violence can be different based on gender. Predictors of ECV in youth is less understood. This study aims to explore potential chronic pathways between initial ECV and continued ECV for early adolescents. The relationship between ECV as a predictor and ECV as an outcome is hypothesized to be mediated by both PTSS and JDB and moderated by gender. Method: A total of 266 African-American, sixth grade students in high crime, high …

Beyond Family: Patterns Of Kin And Fictive Kin Caregivers Among Children And Youth In The Child Welfare System, Jennifer Osborne

Master's Theses

Children and youth in the child welfare system experience significant benefits from placement with custodial kin caregivers in psychological, social, relational, and educational domains (Winokur, Holtan, & Batchelder, 2018; Vasileva & Petermann, 2018). Additionally, the extant literature suggests that non-custodial kin and non-custodial fictive kin, or individuals unrelated by blood or marriage though afforded the same unofficial status as family (Taylor, Chatters, Woodward, & Brown, 2013), also contribute positive outcomes (Smetana, Campione-Barr, & Metzger, 2006). However, little research has examined the ways in which custodial kin, non-custodial kin, and non-custodial fictive kin work together to provide social support to children …

Behavioral Ecology And Physiological Health Of Managed Populations Of Juvenile Blanding's Turtles (Emydoidea Blandingii) In Urban Wetlands, Armand Andre' Cann

Master's Theses

Substantial threats to reptile species biodiversity have become apparent in the last few decades. This is partly caused by significant losses in grasslands and their associate prairie-wetland ecosystems. One Midwestern prairie-wetland species, Blanding's Turtles (Emydoidea blandingii), are at risk of extirpation due to habitat loss, fragmentation, and increased predator populations. Consequently, significant head-starting efforts, a form of reintroduction conservation using captive-reared and then released turtles, have been conducted to recover populations of E. blandingii in northeastern Illinois. However, the success of these programs both locally and range-wide have not been fully quantified. Unfortunately, many approaches to guaging success involve long-term …

Investigating The Nightime Departures Of Glaucous-Winged Gulls (Larus Glaucescens) And The Role Of Social Facilitation, Devon Leigh Mcclain

Master's Theses

Daytime behaviors and occupancy patterns of Glaucous-winged Gulls (Larus glaucescens) have been described and can be mathematically predicted based on environmental factors. However, little is known about the nighttime behaviors of Glaucous-winged Gulls. I used trail cameras to study the daytime and nighttime colony occupancy patterns of Glaucous-winged Gulls on a breeding colony on Protection Island, Washington, USA. Early in the breeding season gulls desert the colony en masse during nighttime even after some gulls have initiated clutches. Using acoustic recording units to identify an acoustic cue that signals the onset of the coordinated nightly departures from the colony, I …

A Study Of Inactive Enzyme-Homologues: The Biochemical And Biological Function Of Ecm14 In Saccharomyces Cerevisiae, Robert Christian Mcdonald

Master's Theses

Like most major enzyme families, the M14 family of metallocarboxypeptidases (MCPs) contains several pseudoenzymes predicted to lack enzyme activity and with unknown molecular function. The genome of the yeast Saccharomyces cerevisiae encodes only one member of the M14 MCP family, a pseudoenzyme named Ecm14 proposed to function in the extracellular matrix. Ecm14 is found throughout ascomycete fungi, with a group of related pseudoenzymes found in basidiomycetes. Although the prodomain of Ecm14 can be cleaved in vivo and in vitro by endopeptidases, suggesting an activation mechanism, no activity has been detected using standard carboxypeptidase substrates.

In order to examine the function …

Intelligent Cinematic Camera Control For Real-Time Graphics Applications, Ian Harris Meeder

Master's Theses

E-sports is currently estimated to be a billion dollar industry which is only growing in size from year to year. However the cinematography of spectated games leaves much to be desired. In most cases, the spectator either gets to control their own freely-moving camera or they get to see the view that a specific player sees. This thesis presents a system for the generation of cinematically-pleasing views for spectating real-time graphics applications. A custom real-time engine has been built to demonstrate the effect of this system on several different game modes with varying visual cinematic constraints, such as the rule …

Human Path Prediction Using Auto Encoder Lstms And Single Temporal Encoders, Hayden Hudgins

Master's Theses

Due to automation, the world is changing at a rapid pace. Autonomous agents have become more common over the last several years and, as a result, have created a need for improved software to back them up. The most important aspect of this greater software is path prediction, as robots need to be able to decide where to move in the future. In order to accomplish this, a robot must know how to avoid humans, putting frame prediction at the core of many modern day solutions. A popular way to solve this complex problem of frame prediction is Auto Encoder …

Black Students, White Schools, And Racism: Exploring The Experiences, Challenges, And Resilience Of Black Students At Private K-12 Predominantly White Institutions (Pwis) Through Adult Reflections, Sade Ojuola

Master's Theses

This project examines the challenging racialized experiences of Black students who attended private predominantly white institutions (PWIs) during their K-12 education, with a particular focus on the long-term impact of those experiences. The existing literature contains valuable data about the experiences of Black students in predominantly white private schools. However, an important gap in the literature exists regarding the reflections and understandings developed over time by Black adults who attended predominantly white private schools. This field project aims to explore the beliefs that were borne of those experiences and how those experiences ultimately become interwoven into a Black student’s identity …

Disordered Eating In Collegiate Athletes: Prevalence And Relationships With Body Composition, Sofia Apsey

Master's Theses

Performance pressures and emphasis on body weight and shape put collegiate athletes at risk for disordered eating. If not identified early, disordered eating behaviors could develop into a clinical eating disorder. While such behaviors are a known problem, prevalence rates are not well established in collegiate athletes. Disordered eating may also relate to physiological changes in athletes, such as body composition and performance, although this relationship is not well understood. The aims of this research were to 1) determine the prevalence of disordered eating risk in NCAA Division I student-athletes and 2) determine if such behaviors are related to baseline …

Representations Of Women And Minorities Groups In Comics, Laticia Donelle Marshall

Master's Theses

The focus of this research is to examine the representation of women and minority groups within comic books. Comic books are a cultural product that involves many actors when it comes to changing the representations of women and minorities. Therefore, this research focused on examining not only literary works but also the actions and contributions of producers and consumers throughout the publication of American comics. In the chapters, I examined the various representations of women, African Americans, Latinos, Native Americans, and Asians in comic books. To conduct my research on changing cultural productions, I used the method of textual analysis …

Electrical Characterization Of Blood Clots, William Mcgrath

Master's Theses

This work reports the use of dielectric spectroscopy to study blood and blood clots. A test setup was developed and electrode behavior was characterized with water and salt solutions. By measuring changes in electrical permittivity, trends differentiating clotting and non-clotting blood were identified. Non-clotting blood exhibited an increase in normalized permittivity at high frequencies that is credited to the sedimentation of red blood cells (RBCs) in static blood samples. Clotting blood was found to show a decrease in permittivity after some time at high frequencies. This is associated with the aggregation of red blood cells. Platelet rich plasma (PRP) and …

Expression And Purification Of Snake Antivenom Peptide In Pichia Pastoris, Israel Juarez Contreras

Master's Theses

It has been shown that a peptide of the first 10-15 N-terminal amino acids of the lethal toxin neutralizing factor (LTNF) protein found in opossums (Didelphis spp.) holds promise as a low-cost therapy for snake envenomation. To date, the 11-mer has been expressed in E. coli and shown to neutralize snake venoms. However, possible endotoxin concerns warrant an investigation into other microbial hosts. The methylotrophic yeast, Pichia pastoris, shows promise as an alternative host. Active LTNF peptide was expressed and purified in P. pastoris. This was accomplished by subcloning a tandem repeat of the first 15 N-terminal amino acids of …

The Relationship Between Flexible Work Arrangements And Work-To-Family Conflict: The Role Of Self-Control, Michelle Ching Klinker

Master's Theses

The purpose of the current study was to examine the relationship between flexible work arrangements and work-to-family conflict. Previous research has not yet examined the possible moderating impact of personal characteristics on this relationship. Therefore, this study also examined self-control as a moderator of the relationship between flexible work arrangements and employees’ experience of work-to-family conflict. A total of 131 participants participated in an on-line survey. Results showed that those who had flexplace and flextime available to them in their organizations tended to experience less work-to-family conflict than those who did not have flexplace and flextime available to them. Furthermore, …

Synthesis And Study Of Verdazyl Stable Free Radical Substituted Oligothiophenes For Magnetoresistive And Spintronic Properties, Amir Shakouri Mansouri

Master's Theses

Interest in spin polarizing materials and their application in electronic devices in the field of spintronics has been steadily growing in recent years. The development of wholly organic spin polarizing materials has been of particular interest due to the material flexibility, solution processing, synthetic modifiability, and longer spin lifetimes of organics compared to inorganic analogs. One class of organic compounds with significant room for exploration is polyconjugated π systems coupled with stable radicals. We have explored the synthesis of oligothiophenes substituted with verdazyl stable free radicals with differing thiophene chain lengths, through a series of halogenation, borylation, and palladium catalyzed …

The Deformability Of Red Blood Cells In Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (Me/Cfs), Brendan Robert Schmidt

Master's Theses

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a disease that causes profound fatigue in its sufferers as well as loss of concentration, headaches, and sleep abnormalities. Unfortunately, the disease is poorly diagnosed, and etiology is not known. Although the ME/CFS patients do not show anemic readings, and their arterial partial pressure is normal, it is possible that their extreme post-exertional malaise stems from poor microvascular perfusion and oxygenation. Previous studies have indicated that the red blood cells (RBCs) in ME/CFS patients show evidence of oxidative damage. We hypothesized that the RBC deformability metrics can serve as a reliable biomarker of ME/CFS …

Summer Habitat Used By American Kestrels In Santa Clara County, California, Nicole Tomes

Master's Theses

Urbanization can be a primary source of the loss of native species populations by causing habitat loss, fragmentation and land conversion (Tilman et al., 2001; Krauss et al., 2010; Marzluff, 2001). Birds of prey, which are mid or top carnivores, are especially at risk from the loss of habitat that can occur on both the breeding and wintering grounds. Raptors can be considered environmental indicators of habitat degradation because of their small populations, low population densities, and high position in local food webs. This research investigated the habitat preference of the American Kestrel (Falco sparverius) (kestrel), a widely distributed raptor, …

Factors Affecting Polymer Brush Thermal Stability, Alexis Sarabia

Master's Theses

Nanostructures, features that are generated on the scale of 1-100 nm, have potential in many applications including semiconductors and sensors. Mixed polymer brushes, which are composed of two or more unique polymer types covalently tethered to a solid surface, have been shown to give rise to distinct nanostructures. The solid support for these systems can be flat surfaces, such as silicon wafers, or 3-dimensional surfaces, such as nanoparticles. Polymer brushes are mechanically and chemically robust, but their thermal stability is not well understood. Because many potential applications require elevated temperatures, understanding and tuning their thermal stability is important. We studied …

Comparing Levels Of Environmental Literacy Between Stars- And Non-Stars-Rated Universities, Analisa Kathleen Campos

Master's Theses

The Sustainability Tracking, Assessment, and Rating System (STARS) is a program that measures university sustainability levels. STARS ranks university campuses based on the number of points received from individual specifications such as green buildings, access to mass transit, number of environmental courses, and more. Therefore, the rank a school receives translates directly to its level of sustainability as an institution. Currently, it is unknown if there is a relationship between a campus’s STARS-rating and individual students’ environmental literacy (EL). The purpose of this project was to analyze the impacts of university’s sustainable features on student EL. Surveys were administered to …

Start-Up Power Requirements Of Ammonia Filled Aerospace Thermosyphon Heat Pipes, Eric Chu

Master's Theses

Boiling and condensing of internal working fluids are essential heat transfer concepts which heat pipe technology relies on for transporting a large amount of heat between two distanced points effectively. Tilt angle and working fluid saturation temperature have a direct impact on how well the evaporator is flooded. This paper focuses on how the tilt angle and the working fluid saturation temperature affect the start-up heat input requirements for an S shaped axial grooved ammonia thermosyphon heat pipe. A series of experiments was performed by supplying and extracting heat to the evaporator and condenser of the thermosyphon heat pipe. Temperature …

Zircon Trace Element Variation From Mono Craters (California) Ashes And Domes As Recorders Of Temporal Changes In The Rhyolite Magma System, Natalie D. Baker

Master's Theses

The Pleistocene to Holocene Mono Craters in eastern California comprise at least 28 high-silica rhyolite domes, lava flows and tephra cones. To examine the geochemical evolution of the magma system, trace elements were analyzed on crystal rims of zircons separated from juvenile pumice clasts from 5 ashes in the correlative Wilson Creek Formation (spanning from 62 to 21 ka) and from 3 rhyolite domes (21, 12.5, and 7 ka). Rare earth element (REE) patterns show similar overall shapes with prominent negative Eu anomalies, indicative of similar geochemical environments of zircon crystallization, but variation in key trace elements and ratios indicate …

Climate-Driven Insect Herbivory In Mixed Coast Live Oak Woodlands Within The Mt. Hamilton Range, Santa Clara County, Michelle Domocol

Master's Theses

Climate change is expected to dramatically alter and destabilize critical functions of oak woodlands (Quercus spp.) in California. Oak woodlands support hundreds of vertebrate species and thousands of native insect species. Climate-driven changes in annual temperature, annual rainfall, and spatial climatic variability may increase insect herbivore pressures on mixed coast live oak (Quercus agrifolia) woodlands. The Mt. Hamilton Range of Santa Clara County, California offers a unique matrix to study oak-insect herbivore relationship using elevation as a proxy for climate change. This thesis research assessed the relationship between lepidopteran herbivory and coast live oak with insect surveys and the laboratory …

Relationship Between Habitat Variables And Leopard Shark (Triakis Semifasciata) Population Trends In The South San Francisco Estuary, Patrice Frazier

Master's Theses

The San Francisco Estuary (the Estuary) supports a diversity of native marine species, including the leopard shark (Triakis semifasciata). As the human population has increased in the Bay Area, elasmobranchs have simultaneously been subjected to habitat loss and changes in hydrology. On a large scale, global climate change is also affecting the Estuary's water quality. There is little research on the long-term population size of leopard sharks in the Estuary. Leopard shark catch data collected by the Marine Science Institute (MSI) and water quality data from the United States Geological Survey (USGS) from 1998 to 2017 were analyzed to assess …

Observations Of Fire Behavior On A Grass Slope During A Wind Reversal, Dianne Hall

Master's Theses

This experiment studied fire-atmospheric interactions and wildland fire behavior on a slope. A grass slope was instrumented with both in situ and remote instruments to record both meteorological conditions and the fire behavior. A headfire was lit and allowed to burn upslope through the instruments. The data collected were analyzed to determine the fire behavior, specifically fire spread (direction and rate) and flame characteristics (length, height, and angle). During the first several minutes of the experiment, fire behavior was as expected with an upslope rate of spread at 0.1 m s-1 and flame lengths between 1 m and 4 m. …

Using Blockchain Technology For The Organ Procurement And Transplant Network, Utsav Jain

Master's Theses

The organ donation system in the United States is centralized and difficult to audit by the general public. This centralized approach may lead to data integrity issues in the future. The Organ Procurement and Transplant Network (OPTN) was built and maintained by a non-governmental organization called the United Network for Organ Sharing (UNOS) under its proprietary UNet(SM) umbrella platform. This platform is made up of proprietary closed source software and does not provide the general public easy access to the organ transplant data for auditing. This study investigates the feasibility, challenges, and advantages of a blockchain-based OPTN.

A prototype of …