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Multi-Assay Nutritional Metabolomics Profiling Of Low Vitamin A Status Versus Adequacy Is Characterized By Reduced Plasma Lipid Mediators Among Lactating Women In The Philippines: A Pilot Study, Catherine M. Johnson

Master's Theses

Background: A significant portion of lactating women in less developed countries have vitamin A (VA) deficiency. Lactation has substantial effects on a mother’s metabolism and VA is known to be needed in multiple biological processes, including growth, vision, immunity, and reproduction.

Objective: The objective of this pilot study was to utilize metabolomics profiling to conduct a broad, exploratory assessment of differences in plasma metabolites associated with low VA status versus adequacy in lactating women.

Methods: Plasma samples from lactating women who participated in a survey in Samar, Philippines, were selected from a cross-sectional study based on plasma retinol concentrations indicating …

Demographic Predictors Of Math Anxiety In Elementary School Children, Melissa Flowerdew

Master's Theses

Due to the increasing interest of math anxiety in elementary level children, the present study explored math anxiety contextual factors through Bandura’s (1999) Social Cognitive perspective. Using the Children’s Anxiety in Math Scale, researchers gathered data from 40 primary-level children to explore four demographic predictors--grade-level, special needs identification, gender identity, and socioeconomic status-- of mathematics anxiety through a hierarchical regression model. Findings indicated demographic variables accounted for over one-third of the variance in math anxiety scores at 38.2% (grade level: 12.3%, SNI: 8.4%, gender identity: 13.2%, SES: 4.1%). With the exception of socioeconomic status, all predictors were significant. These findings …

Long Term Recovery From Cattle Grazing Disturbance And Climate Impacts At Capitol Reef National Park, Utah, Erin Tessens

Master's Theses

Cattle grazing has influenced the environment in the western United States since European settlement in the 1800’s. Continuous and heavy grazing on arid and semi-arid rangelands has resulted in decreased biodiversity, changes in vegetation structure, and vulnerability to exotic plant invasion. Heavy grazing has also been linked to decreased cryptobiotic soil due to trampling and susceptibility to erosion. With a lack of effective means of successful habitat restoration, there is a rising concern among land managers to maintain these intricate systems, notably under the threat of climate change. Consequently, there is a critical need to understand these system’s response to …

Noninvasive Measurement Of Arterial Compliance With A Blood Pressure Cuff Using A Surrogate Arm Bench Top Model For Oscillometric Use, Shane Wilsey

Master's Theses

A surrogate arm model was created that is capable of being used for oscillometry. This model is capable of being used as a bench top model for blood pressure cuff devices. The arm consists of endplates and internal supports that are 3D printed with ABS, a silicone rubber outer sleeve, and interchangeable arteries made from two silicone rubber strips glued together at the edges. The interchangeable arteries have varying compliances that can be used as different inputs for oscillometric testing. A process was established to measure the artery compliances with a curve fit correlation of 0.95. However, testing revealed that …

Comparison Of Two Advanced Oxidation Processes For Their Production Of Hydroxyl Radicals And Evaluation Of A Uv/Ozone Aop At Varying Uv Fluence For Treating Diclofenac, Alexandra Cass

Master's Theses

This study explores the efficacy of two advanced oxidation processes for generation of hydroxyl radicals to promote degradation of emerging contaminants. Drought and water shortage have become pressing issues caused by our world’s changing climate. Water reclamation and reuse are increasingly important options for relieving this water stress. Water reuse runs the risk of reintroducing recalcitrant compounds that can accumulate in our bodies and environment. Advanced treatment methods that degrade these compounds are vital to protect our health and the health of the environment while providing necessary water resources. Advanced oxidation processes (AOPs) have shown great promise for removing recalcitrant …

Hook-Billed Kite, Chondrohierax Uncinatus, Southbound Autumn Migration In Belize And Mexico, Ryan Anthony Phillips

Master's Theses

The Hook-billed Kite (Chondrohierax uncinatus) is a specialized Neotropical raptor with a wide distribution that has received little research attention. The species is considered non-migratory across most of its distribution, but there are scattered records of Hook-billed Kite migration. The present research describes, for the first time, the largest southbound autumn migration of Hook-billed Kite in Belize and Mexico and evaluates the effects of environmental conditions on this phenomenon. Eight years of count data were collected in Belize and 25 years in Mexico. During a total of 3093.1 count hr from 2013-2020 in Belize, 39928 Hook-billed Kites were counted on …

Distribution And Habitat Use Of The Santa Clara County Population Of Red-Bellied Newts (Taricha Rivularis), Joie De Leon

Master's Theses

Anthropogenically-induced habitat loss and degradation have increased extinction rates in amphibians worldwide, yet little is known about many remaining populations. A disjunct population of red-bellied newt (Taricha rivularis), an endemic California species, was discovered 130 km south of its previously known range. Here I document the range and breeding phenology of this population and contrast its mesohabitat use with that of other sympatric newts. Surveys across two years suggest that the southern population of T. rivularis is confined to a 1 km segment of Stevens Creek, and the population follows an early-March to late-April migratory breeding pattern, similar to one …

Humpback Whale, Megaptera Novaeangliae, Feeding Dynamics From The Perspective Of The Individual: Insights From Demography, Life History, And Bio-Logging, Jennifer Tackaberry

Master's Theses

Studies of fission-fusion societies provide a framework in which to compare the feeding dynamics across demographics, unrestrained by stable associations or relatedness. This study used bio-logger data and surface observations combined with long-term population data from the Gulf of Maine humpback whale, Megaptera novaeangliae, population to investigate the influence of demographics on feeding methods, and time spent feeding, and to determine if a coordinated feeding method, kick-feeding, was a cooperative behavior. The results suggest that demographics did influence the feeding method used and highlighted the need to determine how energetic needs change across the feeding season. Adult females did not …

A Meta-Analysis Of Youth Gender Prejudice Interventions, Molly Ackerman-Pulliam

Master's Theses

This meta-analysis of youth gender prejudice interventions provides an estimate of the overall effectiveness of empirical efforts to reduce expressions of gender prejudice or promote gender egalitarian attitudes among child and adolescent samples. Studies eligible for inclusion were quasi- or fully-experimental designs with child and adolescent participants ranging from 5-17 years of age that quantitatively measured gender prejudice reduction or increases in an egalitarian view of gender. The final sample of 31 studies were located via database searches using both general and specific keywords and Boolean operators. Effect sizes for measures of gender prejudice were calculated using Cohen’s d as …

Changes In Allochronic Breeding Populations Of Pink Salmon (Oncorhynchus Gorbuscha) Of Prince William Sound, Alaska, Claire Dormody

Master's Theses

Prince William Sound, Alaska is home to one of the largest pink salmon (Oncorhynchus gorbuscha) fisheries in the world. Salmon populations there have been fished commercially for over a century, and the State’s documentation of commercial fishing provides a valuable dataset in order to examine abundance trends of species in this region. For this thesis research, I analyzed the fluctuations in abundance and isolated even year and odd year breeding populations of pink salmon to assess whether there is a numerical discrepancy between pink salmon that spawn in odd years and the population that spawns in even years. Analysis of …

Urban Agroecology For Food Sovereignty And Biodiversity: Are Urban Community Farms Or Gardens More Effective?, Heidi L. Giancola

Master's Theses

Industrial agriculture is accurately criticized for eliminating biodiversity and destroying food sovereignty. Urban agroecosystems, usually individual plots in community gardens, are promoted to restore ecological services and equity to food systems. Recently, collectively tended urban community farms have developed, with explicit social justice goals. This study directly contrasts the effectiveness of the urban community farm and garden models. Spatial analysis is used to confirm that community farms enhance geographic access for healthy food priority areas compared to gardens in the San Francisco bay area. An online survey of farm staff and gardeners from Marin to Santa Clara County resulted in …

Epiphyte-Host Dynamics Between Pyropia And Nereocystis In Central California, Daniel Joseph Gossard

Master's Theses

Macroalgal epiphytism is widespread, but many unique epiphyte-host interactions remain poorly understood. Pyropia nereocystis is an annual northeastern Pacific species that has evolved to primarily epiphytize the annual kelp Nereocystis luetkeana. I examined three aspects of Pyropia-Nereocystis epiphyte-host dynamics in the southern extent of the host’s range: (1) temporal variation in epiphtyte presence; (2) growth of gametophyte and sporophyte (conchocelis) stages using transplantation experiments; and (3) effects of epiphyte depth and host characteristics on the recruitment and biomass of the epiphyte. Pyropia exhibited a shift in presence on Nereocystis that differed interannually as a function of Nereocystis density and Nereocystis …

Versatile Disubstituted Imidazole Ligands For Suzuki-Miyaura Coupling Under Mild Conditions, Daniel Parker

Master's Theses

Synthesis of small molecules for medicine, agriculture, or research often requires the formation of carbon-carbon and carbon-heteroatoms bonds, and palladium catalyzed reactions have emerged as a potent technique for these cross-coupling reactions. To address increasing catalysts demands, our research has focused on the development of a versatile catalyst platform bearing imidazole pendants as ligands to support cross-coupling reactions under mild conditions, an area of current interest. A series of bridged imidazole ligands was synthesized using a modular approach and characterized by 1H NMR spectroscopy. The catalytic activity of the complexes was measured for cross-coupling reactions in aerobic conditions, over a …

The Relationship Between Different Forms Of Exercise And Adhd Symptomatology, Matthew A. Reynolds

Master's Theses

Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder associated with executive function deficits as well as negative life outcomes in both childhood and adulthood. Current treatments to reduce symptoms of ADHD include stimulant medications that act as catecholamine agonists. These medications often cause undesirable side effects, to which end better understanding the effects of non- pharmacological treatments on symptoms of ADHD is critical. Research conducted in children suggests that exercise may be an effective tool for symptom reduction. However, the relationship between exercise and ADHD symptoms remains unclear for adults. The aim of this study was to address this …

Examination Of Flow Patterns During Droplet Formation And In Venous Valve Mimic Using Μ-Piv, Vignesha Vijayananda

Master's Theses

In this work, particle image velocimetry (PIV) was used for fluid flow visualization in both continuous and segmented-flow microfluidics. Droplet microfluidics is known for its precise and consistent volume dispersion between microliters and picoliters in volume for application ranging from molecular synthesis, drug discover, and diagnostics. But the influence of junction geometry on the process of drop formation has not been investigated. In this study, µ-PIV was used to study the internal flow during the drop formation process in flow-focus microfluidic device with and without constriction. It was found that in case of flow-focus with constriction, the shear force on …

Evaluating Effect Of Folic Acid On Mouse Embryonic Fibroblast Cells With Impaired Dna Repair System, Suneeti Madhavan

Master's Theses

Mandatory folic acid (FA) fortification programs have been introduced in many countries since 1998 [1]. This fortification program, especially when combined with vitamin supplements taken for health purposes, can lead to higher-than-normal levels of synthetic FA in the body [2]. Recent research has linked high levels of synthetic FA (more than the daily requirement of 400μg/day) with increased incidences of cancer [2-7]. In comparison, low levels of FA have been shown to cause DNA damage due to impaired DNA repair processes [8,9]. This study hypothesizes that increased levels of FA give rise to DNA modification. Therefore, if cells harbor an …

The Effects Of Vine Age On Vine Performance, Fruit, And Wine Composition Of Zinfandel In The Templeton Gap Ava, California, Vegas L. Riffle

Master's Theses

A two-year study was conducted at a commercial vineyard in California’s Templeton Gap AVA to evaluate the effect of vine age on viticultural, enological, and sensory attributes of Vitis vinifera L. cv. Zinfandel grapes and wines. The experimental block was dry farmed, conventionally managed, with own-rooted Zinfandel vines that, when determined unproductive, were replaced with new vines of genetically identical scion plant material grafted onto St. George (Vitis rupestris Scheele) rootstock. Treatments included Young vines (5 to 12 years old), Control (representative proportion of young to old vines in the block), and Old vines (40 to 60 years old). …

Analysis Of Dietary Intake, Body Composition And Biomarkers In Adults With Type 2 Diabetes Mellitus, Prediabetes And Without Diabetes, Sarah Thuytrinh Nguyen

Master's Theses

Our study provided an analysis and comparison of specific blood values, dietary intake, body composition, and inflammatory markers (high sensitivity-C-reactive protein (HS-CRP), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6)) between adults with type 2 diabetes mellitus (T2DM) and prediabetes (PDM) to adults without diabetes. A total of 22 participants (PDM/T2DM n=12, controls n=10) in the San Luis Obispo, CA area completed the study prior to our ending recruitment due to Covid-19. Body composition data were collected through DXA scans. Dietary intake was assessed using a 3-day food record survey and nutritional analysis conducted using ESHA food processing software. In addition, …

Analysis Of The Slo Bay Microbiome From A Network Perspective, Lien Viet Nguyen

Master's Theses

Microorganisms are key players in the ecosystem functioning. In this thesis, we developed a framework to preprocess raw microbiome data, build a correlation network, and analyze co-occurrence patterns between microbes. We then applied this framework to a marine microbiome dataset. The dataset used in this study comes from a year-long time-series to characterize the microbial communities in our coastal waters off the Cal Poly Pier. In analyzing this dataset, we were able to observe and confirm previously discovered patterns of interactions and generate hypotheses about new patterns. The analysis of co-occurrences between prokaryotic and eukaryotic taxa is relatively novel and …

Upending The "Racial Death-Wish": Black Gay Liberation And The Culture Of Black Homophobia, Kailyn Pope

Master's Theses

This thesis analyzes the origin and impact of Black homophobia found in activist spaces of mid- to late-twentieth-century American society. Black gay Americans were subjected to intersecting forms of systemic and cultural oppression that were exceedingly hard to escape due to both the homophobia in Black spaces and the racism in gay spaces. Black gay activists and artists thus had to create their own avenues of expression where they and others could fully embrace what it meant to be Black and gay. This work utilizes a Black feminist framework to explore the roots of Black homophobia and how this type …

Metal Dust Cloud Distribution Characterization Through Image Analysis, Shelby Lampshire

Master's Theses

With the increasing development of metal additive manufacturing technology, the present need for accurate explosivity testing of high density and exotic metal powders is under active research. The accuracy of such tests depends upon the uniformity of the dust dispersion within the testing chambers during ignition. There is a need for further research to understand the dust cloud dispersion process in order to determine the best time for ignition. This study explores a methodology of using high-speed footage and image analysis to characterize the uniformity of a dust cloud temporally that future applications may build upon.

This thesis consisted of …

Effects Of Plant Growth-Promoting Bacteria And Fungi On Strawberry Plant Health, Fruit Yield, And Disease Susceptibility, Mary Maher

Master's Theses

Studies on plant growth-promoting rhizobacteria (PGPR) and fungi (PGPF) as biostimulants have shown significant positive effects on plant health, fruit yield, or pest management. However, very few published studies to date have been specific regarding their effects on strawberries (Fragaria × ananassa), particularly on soilborne disease prevalence in organically grown strawberries. Empirical data on the results of using these products in commercial growing applications under various conditions would be highly valuable, especially for organic growers who have limited synthetic chemical pesticides, herbicides and fertilizers registered for use. The objective of this study is to evaluate the efficacy of …

Analysis And Optimization Of A Colorimetric Nanosensor For Rapid Detection Of Escherichia Coli In Water, Sarah M. Stabler

Master's Theses

Safe drinking water is essential for life, yet at least two billion people around the world consume water contaminated with pathogens among other pollutants. Standard methods like polymerase chain reaction (PCR) and membrane filtration have been developed to detect enteric pathogens in water. However, these methods are limited in their accessibility due to long wait times to obtain results, and the requirements of skilled expertise, electricity, and laboratory equipment. This research has focused on addressing some of these limitations by analyzing the mechanisms of work and optimizing an indirect colorimetric nanosensor developed in previous research. The colorimetric nanosensor investigated herein …

Selection Of Overwintering Microclimate By Migratory Western Monarch Butterflies, Kiana Saniee

Master's Theses

Migratory species are expected to demonstrate habitat selection that occurs at multiple spatial and temporal scales. Western monarch butterflies migrate seasonally to overwinter in groves at geographically predictable locations along the California coast. To date, overwintering habitat selection by western monarch butterflies has been studied assuming that habitat selection occurs where overwintering aggregations form, meaning at the spatial scale where monarchs form dense overwintering aggregations within overwintering groves. We argue that since western monarch butterflies are migratory, studies of habitat selection could have commingled selection at different scales into a single spatial scale. This likely leads to ignoring some levels …

Automating Deep-Sea Video Annotation, Hanson Egbert

Master's Theses

As the world explores opportunities to develop offshore renewable energy capacity, there will be a growing need for pre-construction biological surveys and post-construction monitoring in the challenging marine environment. Underwater video is a powerful tool to facilitate such surveys, but the interpretation of the imagery is costly and time-consuming. Emerging technologies have improved automated analysis of underwater video, but these technologies are not yet accurate or accessible enough for widespread adoption in the scientific community or industries that might benefit from these tools.

To address these challenges, prior research developed a website that allows to: (1) Quickly play and annotate …

Evaluation Of Blood Vessel Mimic Scaffold Biocompatibility, Nicole M. Abraham

Master's Theses

The Tissue Engineering Research Lab at California Polytechnic State University, San Luis Obispo focuses on creating tissue-engineered blood vessel mimics (BVMs) for use in preclinical testing of vascular devices. These BVMs are composed of electrospun scaffolds made of an assortment of polymers that are seeded with different cell types. This integration of polymers with cells leads to the need for biocompatibility testing of the polymer scaffolds. Many of the lab’s newest scaffolds have not been fully characterized for biologic interactions. Therefore, the first aim of this thesis developed methods for in vitro cytotoxicity testing of polymers used in the fabrication …

Habitat Heterogeneity Affects The Thermal Ecology Of The Federally Endangered Blunt-Nosed Leopard Lizard, Nicole Gaudenti

Master's Theses

Global climate change is already contributing to the extirpation of numerous species worldwide, and sensitive species will continue to face challenges associated with rising temperatures throughout this century and beyond. It is especially important to evaluate the thermal ecology of endangered ectotherm species now so that mitigation measures can be taken as early as possible. A recent study of the thermal ecology of the federally endangered Blunt-Nosed Leopard Lizard (Gambelia sila) suggested that they face major activity restrictions due to thermal constraints in their desert habitat, but that large shade-providing shrubs act as thermal buffers to allow them …

Examining The Ecological And Social Implications Of Parklets And Plazas Across Multiple Urban Scales, Joshua L. Muller

Master's Theses

Faced with population increases but stagnant capital improvements and impacts from global warming, cities around the world are experimenting with smaller-scale and cheaper strategies in order to accommodate the new influx of residents. New York City has led the way in converting low-efficiency intersection space into public plazas with a limited range of permanently installed elements and San Francisco has pioneered the concept of the parklet, which converts two to four street parking spaces into a modular and flexible pedestrian space. I seek to answer two questions about these spaces: What are common factors influencing the viability and successful implementation …

Microfluidic Electrical Impedance Spectroscopy System Automation And Characterization, Keaton Frahmann

Master's Theses

In this work, a novel microfluidic cell culture platform capable of automated electrical impedance measurements and immunofluorescence and brightfield microscopy was developed for further in-vitro cellular research intended to optimize cell culture conditions. The microfluidic system design, fabrication, automation, and design verification testing are described. Electrical and optical measurements of the 16 parallel cell culture chambers were automated via a custom LabView interface. A proposed design change will enable gas diffusion, removing the need for an environmental enclosure and allow long-term cell culture experiments. This "lab on a chip" system miniaturizes and automates experiments improving testing throughput and accuracy while …

Development Of A Portable Low-Moisture Food Pasteurization Device Using Rf Heating, Eric Jason Ohata

Master's Theses

Bacterial presence in low-moisture foods such as flour, cereals, baby formula, and spices, have become a concern due to sanitizing challenges. The food industry currently focuses on wet food sanitation as opposed to low-moisture foods because of bacteria’s inability to reproduce in low water activity media. Traditionally, food processing RF heating pasteurizes in mass quantities while an equivalent consumer device does not exist the market today. A consumer product would help eliminate food waste by providing an easy way to sanitize food and extend shelf life. The Portable Food Pasteurization (PFP) project is an interdisciplinary project involving the Electrical Engineering, …