Digital Commons Network^™

Computational Pipeline For Human Transcriptome Quantification Using Rna-Seq Data, Guorong Xu

LSU New Orleans Theses and Dissertations

The main theme of this thesis research is concerned with developing a computational pipeline for processing Next-generation RNA sequencing (RNA-seq) data. RNA-seq experiments generate tens of millions of short reads for each DNA/RNA sample. The alignment of a large volume of short reads to a reference genome is a key step in NGS data analysis. Although storing alignment information in the Sequence Alignment/Map (SAM) or Binary SAM (BAM) format is now standard, biomedical researchers still have difficulty accessing useful information. In order to assist biomedical researchers to conveniently access essential information from NGS data files in SAM/BAM format, we have …

The King's Speech: A Rhetorical Analysis Of Shakespeare's Henry Iv, Part I, Chance Sweat

LSU New Orleans Theses and Dissertations

Recent scholarship has explored the “Machiavellian” actions of Prince Hal in Henry IV, Part 1 ; yet the classical rhetorical pedagogy of Renaissance Britain suggests that the speeches in the play lead to a transformation in Hal that is antithetical to the emergent understanding of Hal as a great manipulator. Falstaff uses the ruse of rhetoric instructor in order to construct a classical rhetorical argument for his own ends, and Henry IV gives a passionate yet formally adept (and classically rhetorical) plea to his son in order to incite change. An analysis of Falstaff and Henry’s arguments as well as …

Organizing For Freedom: The Angola Special Civics Project, 1987-1992, Lydia Pelot-Hobbs

LSU New Orleans Theses and Dissertations

During the 1980s and 1990s, the US prison system was expanding at an unprecedented rate. This research charts how prisoners at the nation’s largest maximum-security prison, the Louisiana State Penitentiary, commonly referred to as Angola, founded the Angola Special Civics Project to collectively organize for prison reform. Using a combination of oral history and archival research, this thesis argues that the Angola Special Civics Project emerged during an era of political opportunity created by the coupling of political openings and contractions. Unlike outside advocates who focused their reform efforts on internal conditions, the Angola Special Civics Project centering of prisoners’ …

The Effect Of Tool Rotation Speed And Clamping On Deformation In Friction Stir Welded 6061-T6511 Aluminum Extrusions, Travis Lee Smith

LSU New Orleans Theses and Dissertations

Friction Stir Welding (FSW) was used to perform Bead on Plate (BOP) welds on 6061-T6511 aluminum extrusions. Using a DOE approach, tool rotation speed, clamp spacing, and clamping force were altered to ascertain their effects on distortion in the welded panels. Mechanical forces were monitored during the weld process. Both linear and out of plane distortion were measured on the welded extrusions. The Vickers hardness of the weld nugget was measured. The effect of each parameter on weld distortion was discovered and the mechanism of this link was suggested.

Identification And Characterization Of Enzymes Involved In The Biosynthesis Of Different Phycobiliproteins In Cyanobacteria, Avijit Biswas

LSU New Orleans Theses and Dissertations

A multi-plasmid, co-expression system was used to recreate the biosynthetic pathway for phycobiliproteins from the cyanobacterium Synechococcus sp. PCC 7002 in E. coli. This system efficiently produced chromophorylated allophycocyanin (ApcA/ApcB), -phycocyanin, and -phycocyanin. This system was used to demonstrate that CpcS-I and CpcU proteins are both required attaching PCB to allophycocyanin subunits ApcD (AP-B) and ApcF (18). The N-terminal, AP-like domain of ApcE (LCM99) was produced in soluble form and shown to have intrinsic bilin lyase activity. In addition, this system was used to chromophorylated CpcA from Synechococystis sp. PCC 6803 with a non-cognate bilin; PEB with the aid …

“Emancipation From That Degrading Yoke”: Thomas Jefferson, William Eaton And “Barbary Piracy” From 1784 To 1805, Stacy Meyers

LSU New Orleans Theses and Dissertations

The following essay examines the image of "Barbary piracy" created by two prominent political figures, Thomas Jefferson and William Eaton, and by the American public from 1784 to 1805, and how those images shaped the policy of the American-Barbary War. Eaton‟s Orientalist approach to describing piracy and the North African population limited his views of this region, thus reducing the American conflict to the annihilation of animalistic "brutes." Jefferson‟s practical approach to describing piracy and the North African population focused on emancipating the region from the corrupting influence of greed, allowing him the necessary flexibility to solve the conflict by …

Defending Desire: Resident Activists In New Orleans‟ Desire Housing Project, 1956-1980, Takashi Michael Matsumaru

LSU New Orleans Theses and Dissertations

The Desire Housing Project opened in 1956 as a segregated public housing development in New Orleans‟ Upper Ninth Ward. The Desire neighborhood, one of the few neighborhoods in the city where black homeownership had been encouraged, was transformed by the project. Hundreds of former Desire residents were displaced by the mammoth project, which became home to more than 13,000 residents by 1958. Built on what had once been a landfill, the Desire Housing Project came to epitomize the worst in public housing, before it was torn down by 2001. Although the project was isolated from the rest of the city …

Factors That Affect Harem Stability In A Feral Horse (Equus Caballus) Population On Shackleford Banks Island, Nc, Jessa Madosky

LSU New Orleans Theses and Dissertations

Mammal species often live in social groups, but the factors that promote group cohesion can be difficult to analyze due to the prevalence of strong group affiliations. Feral horses maintain stable harems of one or two males and several females, and harem stability is strongly related to individual fitness. Anecdotal evidence and an early study in the non-breeding season suggest that management of the Shackleford Banks island horses with immunocontraception reduces harem stability in the population, providing an opportunity to study the factors that influence harem stability. I investigated the effects of the immunocontraceptive PZP on harem stability during the …

Will All Of You Stop Being A-Holes, Please?, April Blevins

LSU New Orleans Theses and Dissertations

None

The Relationship Between Graduate Counseling Students’ Meaning In Life And Their Crisis, Lorraine M. Dinkel

LSU New Orleans Theses and Dissertations

Viktor Frankl published Man’s Search for Meaning in 1946, documenting the horrors of the concentration camps. Based on his prison experience in the camps, Frankl (1984) believed that meaning in life could be found in suffering. The theoretical framework for this research study was based on Frankl’s theory of logotherapy, an extension of existentialism. In today’s society, we can find many parallels to Frankl’s descriptions of suffering in the natural and human-made disasters that have occurred such as the 1999 shooting at Columbine, the levee failure in 2005 following Hurricane Katrina, the floods in the spring of 2011 in the …

Synthesis And Characterization Of Nanostructures In Porous Anodic Aluminum Oxide Templates, Jin-Hee Lim

LSU New Orleans Theses and Dissertations

In this study, template-based methods are used for the fabrication of various nanostructures such as nandots, nanorods, nanowires, nanotubes, and core-shell structures. Porous alumina membranes were employed as templates and metal nanostructures were synthesized in the templates by electrodeposition. By using lithography techniques, controlled patterned nanostructures were also fabricated on alumina templates. The magnetic properties of the various metal nanostructures were investigated. The pore size, interpore distance, and pore geometry highly affect magnetic properties of nanostructures grown in the templates. Hexagonally ordered porous alumina templates can be fabricated by two-step anodization. The pore diameters and interpore distances were readily controlled …

Subsurface Controls On Mainland Marsh Shoreline Response During Barrier Island Transgressive Submergence, Mary Ellison

LSU New Orleans Theses and Dissertations

Many recent studies have sought to understand the response of barrier islands and their attendant marshes to sea level rise. The Mississippi River delta plain, specifically the Chandeleur Islands and associated interior wetlands in eastern Louisiana, serves as an excellent natural laboratory for studying these responses. This region is presently undergoing the highest rates of shoreline erosion (> 15 m yr -1) in North America as wetlands are converted to open water in a regime of subsidence-driven rapid relative sea-level rise (~1 cm yr-1). Three conceptual models were developed based on the geomorphic relationships observed in the marsh that describe …

Neuroendocrine Function Of Female Youth With Callous-Unemotional Traits, Andrew Gostisha

LSU New Orleans Theses and Dissertations

Callous-unemotional (CU) traits have been shown to designate a particular subgroup of antisocial youth that are particularly violent, recidivistic, and more likely to continue offending in adulthood. Disordered neuroendocrine function may be a mechanism for the development of CU traits. We examined whether altered stress responsivity served as a mechanism linking stress exposure and the expression of CU traits. Participants were 15 incarcerated adolescent girls with CU traits. Measures of CU traits, stress exposure, and salivary cortisol were collected. Results revealed girls with CU traits had higher morning levels of cortisol, an intact cortisol awakening response (CAR), and flatter diurnal …

Dark Consciousness: Theory Of Mind And Henry James’S The Golden Bowl, Adam Maillet

LSU New Orleans Theses and Dissertations

Using the psychological concepts of Theory of Mind and embodied cognition, the author explores and questions the traditional readings of Henry James's novel, The Golden Bowl, and its protagonist, Maggie Verver. Although the majority of critics view her as a positive character, James takes great effort to subvert her thoughts and mislead the reader. Despite lacking a modern technical vocabulary, James remains acutely aware of how human cognitive structures both process a text and function within a social setting.

Out Of True, Andrew David Bryan

LSU New Orleans Theses and Dissertations

In this paper, I will detail the process that went into the making of my thesis film, Out of True. The areas I will cover include Writing, Directing, Production Design, Cinematography, Editing, Sound, as well as Technology and Workflow. Special emphasis will be given to Directing and the new directing style I experimented with in an effort to create not only believable but engaging performances. I will then assess the success of this experiment through the use of audience questionnaires.

Metabolism And Cryo-Sensitivity Of Domestic Cat (Felis Catus) And Cheetah (Acinonyx Jubatus) Spermatozoa, Kimberly Terrell

LSU New Orleans Theses and Dissertations

Teratospermia (ejaculation of ≥ 60% structurally abnormal spermatozoa) is prevalent among felids facing extinction risk, including the cheetah. This trait also occurs in certain domestic cat populations, providing a valuable research model. Multiple components of sperm function are disrupted in teratospermic cats, and even structurally normal spermatozoa from these ejaculates may be functionally compromised. Teratospermic ejaculates are highly sensitive to damage during cryopreservation, limiting the success of genome resource banking programs for species conservation. Although both teratospermia and cryopreservation are linked to disruptions in multiple energy-dependent sperm processes, the metabolism of these cells has not been investigated. This project explored …

Freecell And Other Stories, Susan Louvier

LSU New Orleans Theses and Dissertations

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Simin Daneshvar's Savushun: Examining Gender Under Patriarchy, Yasaman Jahed

LSU New Orleans Theses and Dissertations

The author covers issues of gender and Iranian national identity as reflected in Iran’s first published woman novelist, Simin Daneshvar. Her novel, Savushun, is the first novel to be published by an Iranianwoman in 1969. The novel depicts Iran at the start of the country’s governmental factions in 1941 when Reza Shah Pahlavi overthrew years of Iranian dynasty and established a monarchy. This thesis explores how the novel is a vital part of Iran’s historical literature as well as essential to the present day discussion of gender and politics, especially for women within the patriarchal paradigm.

Sea: A Novel Computational And Gui Software Pipeline For Detecting Activated Biological Sub-Pathways, Thair Judeh

LSU New Orleans Theses and Dissertations

With the ever increasing amount of high-throughput molecular profile data, biologists need versatile tools to enable them to quickly and succinctly analyze their data. Furthermore, pathway databases have grown increasingly robust with the KEGG database at the forefront. Previous tools have color-coded the genes on different pathways using differential expression analysis. Unfortunately, they do not adequately capture the relationships of the genes amongst one another. Structure Enrichment Analysis (SEA) thus seeks to take biological analysis to the next level. SEA accomplishes this goal by highlighting for users the sub-pathways of a biological pathways that best correspond to their molecular profile …

Swift And Stewart: The Societal Background And Influence Of Satirists In Turbulent Times, Jon Nathan Raby

LSU New Orleans Theses and Dissertations

In this paper, I consider the success of Jonathan Swift’s The Drapier’s Letters and Jon Stewart’s The Daily Show in changing the political climate of the world around them. By analyzing the political background of America in the 2000s and the Irish reaction to William Woods’ patent in the 1720s, I prove the influence of Stewart and Swift’s satire. I then analyze the specific tactics each employs in order to achieve an audience and influence change, concluding by comparing the similar tactics that each use, including persona, irony, and humor as a veil of serious intent.

Towards The Synthesis Of Magnesidin, Subramanya Pingali

LSU New Orleans Theses and Dissertations

Magnesidin is a magnesium chelate of the 3-hexanoyl and 3-octanoyl tetramic acid derivatives isolated from Psuedomonas magensiorubra. Its activity against grampositive bacteria was found to be a specific target for Gingivitis, a dental plaque.Although the synthesis of magnesidin has been reported earlier, it was not reproducible. The highly polar nature and it’s ability to exhibit tautomerization makes their chemical behavior complex and difficult to predict its structure. A variety of reactions and an in depth understanding of the chemical structure is necessary to attain the synthesis of these compounds. This dissertation focuses on addressing the attempts towards the synthesis …

Lily Bart And Isabel Archer: Women Free To Choose Lifestyles Or Victims Of Fate?, Heidi Elizabeth Braden

LSU New Orleans Theses and Dissertations

This thesis argues that Isabel Archer of Henry James’s novel The Portrait of a Lady and Lily Bart of Edith Wharton’s novel The House of Mirth were nineteenth-century characters struggling to assert their social and sexual independence in a male dominated society. Although Isabel inherits a fortune that allegedly enables her to have more autonomy than Lily, both characters are negatively affected by their inability to conceive of their lives outside of social convention.

A Study On Remaining Useful Life Prediction For Prognostic Applications, Gang Liu

LSU New Orleans Theses and Dissertations

We consider the prediction algorithm and performance evaluation for prognostics and health management (PHM) problems, especially the prediction of remaining useful life (RUL) for the milling machine cutter and lithium ‐

ion battery. We modeled battery as a voltage source and internal resisters. By analyzing voltage change trend during discharge, we made the prediction of battery remain discharge time in one discharge cycle. By analyzing internal resistance change trend during multiple cycles, we were able to predict the battery remaining useful time during its life time. We showed that the battery rest profile is correlated with the RUL. Numerical results …

Synthesis Of Chiral Intermediates By Deritivization Of Monosaccharides, Sanjeeva Dodlapati

LSU New Orleans Theses and Dissertations

Conformationally constrained bicyclic amino acids are invaluable in the synthesis of natural products and peptidomimetics. Aeruginosins contain novel bicyclic amino acid, 2--‐carboxy--‐6--‐hydroxyl octahydrindole (Choi) as the core structure. Aeruginosins are tetra peptide serine protease inhibitors isolated from marine sponges and cyanobacterial water blooms. Rigid bicyclic amino acid(Choi)is an essential core structure, which strongly influences biological activity of aeruginosin family members. Aeruginosins showed promising inhibitory activity against thrombin, trypsin, and factor VIIa. Thrombin and factor VIIa play a major role in blood clotting cascade; excessive coagulation leads to thrombosis and other cardiovascular diseases. Several research groups have reported a number of …

Pulsed Laser Deposition Of Thin Film Heterostructures, Ezra Garza

LSU New Orleans Theses and Dissertations

Thin films of Strontium Ruthenate have been grown on Strontium Titanate and Lanthanum Aluminate (100) substrates by pulsed laser deposition. X-ray diffraction results show that the films grown on the Strontium Titanate are amorphous and polycrystalline on the Lanthanum Aluminate. Resistances versus temperature measurements show that the films exhibit semiconducting characteristics. In addition to the growth of Strontium Ruthenate thin films, multilayer heterostructures of Terfenol-D thin films on polycrystalline Lead Titanate thin films were grown by pulsed laser deposition. By using a novel experimental technique called magnetic field assisted piezoelectric force microscopy it is possible to investigate the magnetoelectric coupling …

Enhancing The Verification-Driven Learning Model For Data Structures With Visualization, Yashwanth Reddy Kondeti

LSU New Orleans Theses and Dissertations

The thesis aims at teaching various data structures algorithms using the Visualization Learning tool. The main objective of the work is to provide a learning opportunity for novice computer science students to gain a broader exposure towards data structure programming. The visualization learning tool is based on the Verification-Driven Learning model developed for software engineering. The tool serves as a platform for demonstrating visualizations of various data structures algorithms. All the visualizations are designed to emphasize the important operational features of various data structures. The learning tool encourages students into learning data structures by designing Learning Cases. The Learning Cases …

Topochemical Manipulation Of Layered Perovskites, Elisha A. Josepha

LSU New Orleans Theses and Dissertations

Topochemical strategies, techniques that allow one to effectively manipulate the structures of nonmolecular solids once a crystal lattice is established, are effective in the low temperature (< 500 °C) modification of solid state structures, allowing the preparation of nonmolecular compounds not accessible by standard synthetic routes. Some of the techniques, ion exchange, intercalation/deintercalation, have proven to be excellent synthetic methods for preserving specific frameworks. The combination of these techniques can allow one to create a multistep approach that can be used to design new compounds with interesting properties.

As an expansion to the field of topotactic reactions, a multistep approach was developed towards the synthesis of the new compounds (A xM0.5Cly)LaNb2O7 (where A = Rb, Cs; M = Fe, Ni; x ≈ 1.5;y ≈ 1) at temperatures below 400oC. The first reaction step involved the ion exchange of the host materials (ALaNb2O7, A = Rb, Cs) to form the products M0.5LaNb2O7 (where M = Fe, Ni), a structure open to further …

Grand Isle, Bruce Horack

LSU New Orleans Theses and Dissertations

A novel about a man injured while working on an oilrig in the Gulf of Mexico, set primarily in Louisiana, Nevada, and California. While recovering from his injury, the protagonist is contacted by his dead brother’s daughter—a person whom he did not know existed—and he journeys to San Francisco in search of her.

Thermoelectric Properties Of Zr0.5hf0.5ni1-Xpdxsn0.99sb0.01 And Effect Of Nanoinclusions On Transport Properties Of Half Heuslers, Rumana Yaqub

LSU New Orleans Theses and Dissertations

Thermoelectric materials convert temperature gradients into electricity and vice-versa. These materials utilize the Seebeck effect for power generation and function without moving parts and are highly reliable. The efficiency of thermoelectric devices is related to the dimensionless figure of merit for the constituent materials, defined as where S is the Seebeck coefficient, is the electrical conductivity, is the thermal conductivity and T is the temperature. Maximizing ZT is very challenging because of interdependence of parameters, for example, increasing the electrical conductivity by increasing the carrier concentration invariably lowers S and vice versa. Presently numerous thermoelectric materials are being investigated by …

Water In Protein Cavities: Free Energy, Entropy, Enthalpy, And Its Influences On Protein Structure And Flexibility, Hongtao Yu

LSU New Orleans Theses and Dissertations

Complexes of the antibiotics novobiocin and clorobiocin with DNA gyrase are illustrative of the importance of bound water to binding thermodynamics. Mutants resistantto novobiocin as well as those with a decreased affinity for novobiocin over clorobiocinboth involve a less favorable entropy of binding, which more than compensates for amore favorable enthalpy, and additional water molecules at the proteinligandinterface.Free energy, enthalpy, and entropy for these water molecules were calculated by thermodynamicintegration computer simulations. The calculations show that addition of thewater molecules is entropically unfavorable, with values that are comparable to the measuredentropy differences. The free energies and entropies correlate with the …