Digital Commons Network^™

The Communal Integration Of Nutrition-Related Cultural Assets Of The Latinx Population In The Greater Dayton Area, Hannah G. Waters

Honors Theses

Due to a series of social determinants, the Latinx population currently living in the United States is suffering from a multitude of health disparities. Many of these disparities are nutrition-related, as the health issues that most disproportionately affect the Latinx population are obesity and diabetes. In order to treat these dietary-related health disparities, one must examine the complex aggregation of factors that work to influence specific dietary habits. Dietary habits are impacted by a series of individual, social and environmental factors. Moreover, in the case of Latinx nutrition, the level of acculturation, or cultural adjustment, plays a crucial role in …

How To Defend Those Who Defend: An Examination Of The Underfunding Of The Public Defender System, Selma M. Newbill

Honors Theses

The public defender system in the United States is in an indigent defense crisis because it is often unable to provide adequate representation to the citizens that the United States Constitution requires them to give. The growing attention on the system today is shedding light on public defenders’ stifling caseloads and on the people who are failing to receive the legal representation to which they are entitled by the constitution. The lack of political prioritization, the systemic inequities throughout the criminal justice system, and the underfunding of the public defender system has often rendered public defenders unable to provide their …

Investigating Pirna Expression And Function In Regenerating Tissue Of Segmented Annelid Capitella Teleta, Beatriz Schueng Zancanela

Honors Theses

Regeneration can be observed virtually in all animals and previous studies have identified numerous genes involved in this process. In some invertebrates, the P-element induced wimpy testis (PIWI) genes appear to have an essential role. One such organism is Capitella teleta, a widely accepted model for annelid development. PIWI proteins are associated with small non-coding RNA called PIWI-interacting RNAs or piRNAs, which are involved in transposon silencing in the germline cells of many animals. It was previously believed that these proteins were only expressed in germline cells, however, recent studies have shown expression in somatic tissues as well. The function …

The Impact Of Oxygen Availability And Oxidative Stress On Regulation Of Cyclic-Dimeric-Gmp In Listeria Monocytogenes Strain F2365, Anna G. Welch

Honors Theses

Listeria monocytogenes is a gram-positive foodborne pathogen that results in the infectious disease listeriosis. The second messenger molecule cyclic-dimeric-GMP has been found to be responsible for the regulation of expression of many of its virulence factors with diguanylate cyclases and phosphodiesterases regulating the intracellular concentration of cyclic-di-GMP through synthesis and degradation, respectively. This study investigated the possibility that the availability of oxygen is the environmental signal crucial to the regulation of these enzymes. Prior research in our laboratory has demonstrated that the intracellular concentration of cyclic-di-GMP is elevated when exposed to anaerobic conditions. Due to this, it was hypothesized that …

Biochemical And Computational Characterization Of Small Regulatory Rnas In The Eastern Oyster, Crassostrea Virginica, Isabelle Townsend

Honors Theses

RNA interference (RNAi) is a process by which gene expression is regulated using small non-coding RNAs (ncRNAs). Three classes of ncRNAs, including micro-RNA (miRNA), short-interfering RNA (siRNA), and Piwi-interacting RNA (piRNA), are readily distinguishable in eukaryotic systems based on unique characteristics such as read sizes, overlap signatures, and mode of biogenesis. In this study, a method for purification of small RNAs was explored in the eastern oyster, Crassostrea virginica. This method involved the use of Sepharose beads for anion exchange chromatography to enhance purification of Argonaute associated small RNAs. Following RNA extraction and purification, small RNA libraries were created …

Effects Of Patient’S Race On Pain Perception And Treatment In Nursing Students, Christian J. Phillips

Honors Theses

This study investigates whether a patients’ race affects how nursing students evaluate the patient’s pain. Undergraduate and graduate nursing students (N = 117) recruited from the University of Southern Mississippi School of Nursing were presented with a clinical vignette detailing a 35-year-old man in the emergency department presenting with extreme left shoulder pain. They were randomly assigned to either a Black or a White patient condition. The patient’s race was revealed through an attached photograph, with each condition represented by one of eight unique photographs. Participants evaluated the patient’s current pain level and time to be triaged; the patient’s …

Effects Of Prescribed Fire On Soil Respiration In A Longleaf Pine Forest., Caroline E. Paxton

Honors Theses

Prescribed fire is a common tool used to increase the herbaceous diversity in longleaf pine forest understories and to eliminate competition from undesirable mid-canopy species. Little is known about the effects of these fires on the soil respiration rates within these forests. A study of the effects of prescribed fire on soil respiration was conducted within a longleaf pine stand at the Lake Thoreau Environmental Center to examine soil respiration across seasons and before and after a prescribed fire. Soil CO₂ efflux rates were measured using a LICOR LI-8100A gas flux system with long-term chambers from October 2020 to …

Unraveling The Mummy: The Effects Of Natural Mummification On The Recovery And Degradation Of Dna, Marissa Gandolfo-Muller

Honors Theses

As demonstrated through casework and research studies (Hawass et al., 2010; Gielda & Rigg, 2017), anthropogenic mummification and modern-day embalming can expedite degradation of DNA. Current research in the field of forensic mummification is sparse and little research has been done on quantifying naturally mummified DNA (Leccia et al., 2018; Shved et al., 2014). This research focuses on observing and quantifying the differences in the recovery and degradation of DNA from specimens that have been naturally mummified. This research on natural, forensic mummies is a blend of experimental archeology and postmortem DNA analysis.

In this study, two control specimens and …

Study Of The Role Of The Msaabcr Operon In Tricarboxylic Acid Cycle Activity And Persister Cell Formation In Staphylococcus Aureus, Karsen Motter

Honors Theses

Staphylococcus aureus is an important human pathogen that causes wide arrays of infections ranging from minor skin infections to lethal systemic conditions such as infective endocarditis, osteomyelitis, sepsis and pneumonia. These systemic diseases are often difficult to treat due to the presence of persister cells. Persister cells are a phenotypic variant of the bacterial population that exhibit extreme and transient antibiotic tolerance accompanied by a transient halt in growth. Upon cessation of antibiotic treatment, however, persisters resume growth which results in recurrence of infections. This characteristic of persister cells therefore displays high clinical significance. In this study, we show the …

Vertebrates At The University Of Southern Mississippi: A Wildlife Survey Of The Bear Point Bayou And Surrounding Gulf Park Campus, Hannah Mcduffie

Honors Theses

This project originated through interest in pursuing a field-based research project involving vertebrates. After conducting literature reviews, it was found that the Bear Point Bayou running through the University of Southern Mississippi Gulf Park Campus has never been thoroughly surveyed for species richness (Mohrman et al. 2016). Four sites on the Gulf Park Campus were chosen and vertebrate species were documented and identified through observations, camera trapping, and vocalization recordings. These data, along with species previously recorded on campus, were combined, and compared to data from the Gulf Coast Phenology Trail of which the Gulf Park Campus was recently added …

The Diversity And Evolution Of Phenazine Biosynthesis Pathways In Enterobacterales, Christian Leise

Honors Theses

Enterobacterales is an order of Gram-negative bacteria that encompasses plant and animal pathogens and organisms of industrial importance. Some of these bacteria produce secondary metabolites classified as phenazines (Phz). Studies in other groups of microorganisms revealed that phenazines are redox-active and exhibit broad antibacterial, antifungal, and antiparasitic activity. Enterobacterales are known to produce phenazines, but details about the diversity, biochemistry, and function of phenazine metabolites in these organisms are missing. In this work, we screened the National Center for Biotechnology Information (NCBI) GenBank for genome sequences of phenazine-producing (Phz+) Enterobacterales. Additionally, genomes of Phz+ strains Pectobacterium carotovorum cc303 and …

Synesthesia: Does It Have An Effect On Memory?, Elizabeth Evans

Honors Theses

Synesthesia is a perceptual phenomenon involving an inducer (a stimulus) and a concurrent (elicited response). For many years, scientists have striven to explain synesthesia and whether or not there are benefits to the people who experience it. In this study, nineteen participants were asked to complete a questionnaire in an attempt to see if their synesthetic experiences gave them advantages in the area of memory. The seven questions were given in a multiple-choice or text entry format, and the questionnaire was approved by The University of Southern Mississippi’s IRB before being sent out in an electronic format. The majority of …

Interregulation Between Msaabcr Operon And Ccpe To Determine Staphylococcal Metabolism And Virulence, Erin R. Cox

Honors Theses

Staphylococcus aureus is a complex human pathogen that causes problems in both healthcare and community settings. Staphylococcus aureus is a gram-positive, sphere shaped bacterium that usually colonizes in the nasal cavity of healthy individuals. Staphylococcus aureus infections are a growing health concern due to its ability to produce virulence factors, such as pigmentation, protease production, and capsule formation. Two regulators in S. aureus virulence factors are msaABCR, which is a newly characterized operon, and ccpE. In order to determine the interaction between these two regulators in regulating virulence and metabolism in S. aureus, ccpE and ccpE/ …

Mitochondrial Replacement And Mitonuclear Interactions In The Broadstripe Topminnow (Fundulus Euryzonus), Auburn Ansley

Honors Theses

When species hybridize, mitochondrial and nuclear introgression can take place. This is commonly observed in freshwater fish species, such as the Fundulus notatus complex, which is composed of F. notatus, F. olivaceus, and F. euryzonus. The broadstripe topminnow, F. euryzonus, is only found in the Amite and Tangipahoa Rivers in the Lake Pontchartrain drainage, where it overlaps in distribution with F. olivaceus. Previous studies determined that F. euryzonus in the Tangipahoa River system possesses mitochondrial DNA (mtDNA) from F. olivaceus as a result of hybridization between the two species. The goal of this thesis was …

Phosphodiesterase D Is Involved In Bile Resistance In Listeria Monocytogenes, Sophia A. Ali

Honors Theses

Listeria monocytogenes is a deadly foodborne bacterium that is responsible for almost 20% of food-related deaths in the United States. Listeria monocytogenes contaminates ready-to-eat products such as cheese, deli meat, and ice cream. Once ingested, it invades the intestinal lining and can enter the bloodstream, causing listeriosis. There is a gap in the knowledge of the pathogenesis of L. monocytogenes in how it is able to survive in the gastrointestinal tract in the presence of bile, which has bactericidal properties. Previous studies have suggested that the second messenger cyclic-dimeric-GMP may be involved in the regulation of virulence factors of Listeria. …

Assessing The Occurrence Of Trichechus Manatus Latirostris In Mobile Bay, Alabama Using Edna, Elora Pierce

Honors Theses

In the past several decades Florida manatees (Trichechus manatus latirostris) have begun to expand their summer range into the northern Gulf of Mexico. Because this is a recent occurrence, not much is known about their habitat use and distribution in this region. Citizen-sourced sighting data suggests that Florida manatees frequent subembayments of Mobile Bay, Alabama, reaching a sighting peak in August. To assess the occurrence of manatees in this area environmental DNA surveys were used from winter (February 19-20) and summer (August 21-22) of 2018. At each of the 21 sites ranging from the mouth of Mobile Bay, …

Utilizing The Oxygen-Barrier Properties Of Poly(Isobutylene) To Enhance Photon Upconversion In Thermoplastic Elastomers, Haley Carroll

Honors Theses

Upconversion (UC) is the conversion of low energy light to higher energy light. It is utilized for a broad range of applications such as solar energy harvesting, microscopy of cellular biology, and cancer therapy. Specifically, triplet-triplet annihilation UC (TTA-UC) involves two dye species, a sensitizer and an annihilator, which are capable of upconverting noncoherent light such as sunlight. Traditionally, TTA-UC is achieved in solution, rubbers, or glasses, however, this work probes the capabilities of a thermoplastic elastomer (TPE) matrix to examine the effect of phase-separated morphology on the energy transfers necessary for TTA-UC. The system herein consists of poly(styrene-isobutylene-styrene) (SIBstar) …

Investigating Subcellular Localization Of Somatic Piwi Proteins In The Annelid Capitella Teleta, Tierra D. Cooper

Honors Theses

PIWI proteins are well known for their various roles and localization in germline development of diverse organisms, yet their specific role and function in somatic tissue remains elusive. PIWI proteins are essential for the biogenesis of PIWI-interacting RNA (piRNA), silencing mechanisms, sexual reproduction, and regeneration. piRNAs are PIWI bound RNAs that play an essential role in transposon silencing. PIWI and piRNAs expression and localization have been discovered in the model organism Drosophila melanogaster and mice; however, its localization in the C. teleta annelid is currently unknown and the subject remains an active area of research. In the present study, the …

Phenotypic Characterizations Of Msaabcr Operon Deletion In Staphylococcus Epidermidis Rp62a, Raelyn Williams

Honors Theses

Staphylococcus epidermidis is a human pathogen that is increasingly known for its role in hospital infections associated with implantable medical devices. Antibiotic resistance has become a concerning issue for these infections as this bacteria have various virulence traits that help to evade immune response and antibiotic treatment. Currently, the most effective way to treat S. epidermidis infection is removal of the implant and long-term antibiotic treatment. S. epidermidis causes infection by expressing several protein factors that induce biofilm formation, the bacteria’s primary virulence mechanism. The purpose of this study was to perform the phenotypic characterizations of the msaABCR operon in …

The Role Of Gac/Rsm Signal Transduction Pathway In The Biology Of Antagonistic Marine Bacterium Gynuella Sunshinyii 449, Anhthu Truong

Honors Theses

Gynuella sunshinyii is a newly characterized bacterium with a remarkable ability to produce multiple secondary metabolites that act as potential antibiotics for medical applications. Currently, little is known about gene regulation in this unusual microorganism. In 2018, studies conducted of rhizosphere microbiomes of tidal marsh grasses on Deer Island, MS, resulted in multiple isolates of halophilic, aerobic, Gram-negative bacteria with antagonistic properties. One of the most active antagonists was identified as Gynuella sunshinyii 449. The genome of strain 449 was sequenced with Illumina MiSeq, assembled with Unicycler and SPAdes, and annotated with RASTtk. The annotation revealed a 6.09 Mb genome …

The Role Of Parenting Practices In Adolescent Aggression And Impulsivity, Elizabeth G. Payne

Honors Theses

Parents have an influential role in adolescent development. Previous findings have found a relationship between parenting practices and adolescent behavioral problems. The purpose of this study was to analyze different parenting practices’ influence on impulsive and aggressive behaviors in adolescents. This study uses data gathered from a unique population of at-risk adolescents from a military-style bootcamp who were asked about their parents’ behaviors and their own behaviors. Findings from this study expand upon previous research on parenting practices by analyzing a specific population of at-risk youth and looking at specific forms of impulsive and aggressive behaviors. Data was collected through …

Molecular Characterization Of Galectin From Amblyomma Americanum In Context Of Α-Gal Syndrome, Sumar Beauti

Honors Theses

The lone star tick Amblyomma americanum is a vector of various disease-causing pathogens and tick-borne alpha-gal syndrome (AGS) with rapidly expanding populations in the south- and northeast regions of the United States. This study aimed to molecularly characterize galectin and determine its involvement in galactose-α-1,3-galactose (α-gal) synthesis, transport, reproductive fitness, and microbial homeostasis in this tick. The lone star tick galectin possesses two conserved carbohydrate recognition domains and shares homology with other Ixodid tick galectins. Time and tissue-dependent expression data shows that galectin is constantly expressed in salivary glands, midgut, and ovary tissues. An RNA interference approach was used to …

Energy Therapies In Nursing Practice: Effects, Barriers, And Limitations, Sydney J. Stone

Honors Theses

Complementary and alternative medicine (CAM) is a growing field of holistic treatment modalities that aim to promote health and well-being or treat illness. Energy therapies are a subsection of CAM that have the potential to revolutionize the way that nurses manage their patient’s comfort. Past research has noted that more money is spent in the United States each year on CAM than is spent on conventional care, and researchers have identified the high spending as a discrepancy due to CAM’s general inaccessibility. This study aimed to analyze the existing research and evidence pertaining to the effects of energy therapies and …

Leadership, Job Stress And Uncertainty Among Nurses During The Covid-19 Pandemic: Impacts And Implications In Lieu Of Pertinent Theoretical Constructs, Davis Woodson

Honors Theses

The purpose of this study is to determine the effect of the COVID-19 pandemic on practicing nurses in the United States. The study considered the effect of communication, self-efficacy, intolerance to uncertainty, and life satisfaction on nurses’ job satisfaction; additionally, this study considered the extent to which nurses perceived organizational response efficacy was predicted by their perceptions of communication and perceived threat susceptibility. A total of 191 nurses participated in the online survey. The study revealed that life satisfaction was positively predicted by communication, self-efficacy, and life satisfaction in multiple regression analyses. Perceived communication positively predicted perceived organizational response efficacy …

Emotional Intelligence As A Moderator Of The Relationship Between Psychopathic Personality Traits And Relational Aggression, Aria T. Smith

Honors Theses

Psychopathy is a robust predictor of aggressive behavior and psychopathic personality traits have been shown to predict relational aggression in non-clinical samples of college students. Given the manner in which emotional intelligence appears to be useful in navigating complex social interactions, some have suggested that it may be involved in certain forms of deceitful or manipulative behavior, including those that may be part of relational aggression. The current project evaluated the role of psychopathic personality traits and emotional intelligence in the prediction of relational aggression among college students. In addition to examining the direct relationship of these variables to relational …

“Sticks And Stones”: Experiencing Microaggressions From The Perspectives Of The Victim, Bystander, And Perpetrator, Emily Halvorson

Honors Theses

For over thirty years, microaggressions have been studied for their weight on members of groups like ethnic and racial minorities, women and members of the LGBTQ community. Microaggressions are the routine, derogatory interactions like slights gestures, snubs or minor insults. Microaggressions yield physical and psychological distress to victims and communicate to marginalized groups the biases and prejudices against them harbored by majority group members. My thesis study explored factors such as quality of psychical and psychological health, physical health symptoms, occurrence of physical pain, resilience, and self efficacy and relationships with racial, gender, and sexual orientation-based microaggressions. I analyzed these …

Breast Cancer Treatment Causing Cardiovascular Complications: How Can We Prevent It?, Abigail L. Reese

Honors Theses

Breast cancer has the highest incidence rate of any cancer in the United States, and it is the second most deadly. It comprises 30% of all diagnosed cancers in women, and it is predicted that the incidence of breast cancer will continue to rise. Most common chemotherapeutic agents utilized in the treatment of breast cancer are known to be cardiotoxic, each with differing mechanisms and possible damage done to the patient. In turn, cardiovascular diseases are the number one killer of women in the United States, and their prevalence is continually growing. It is unknown exactly how many women who …

An Analysis Of The Pollinators Of Echinacea Purpurea In Relation To Their Perceived Efficiency And Color Preferences, Carmen Black

Honors Theses

This study aimed to better understand how insects interacted with species of Echinacea in Tennessee and specifically their preference to floral color. Based on previous studies I expected the main visitors to be composed of various bees, beetles and butterflies. Based on previous studies, I hypothesized that most bees (especially social bees) would most likely pollinate the purple/violet morphs of the flower versus the white morph. I also expected the bees would have the most visits to the coneflowers versus any other taxonomic group. I believe bees to be the majority of pollinators based upon the results of Stucky, Gadd, …

Design During A Pandemic: Application Of The Well Building Standard To Historic Engel Stadium, Laurel Getty

Honors Theses

Can buildings prevent the spread of disease through their design? This study argues that by employing the WELL Building Standard to an adaptive reuse project during the COVID-19 pandemic, human health and wellbeing is increased. Research shows that adapting existing buildings to the WELL Building Standard improves air quality, promotes clean contact, and strengthens immune systems (The International WELL Building Institute, 2020). Additionally, adapting existing historic structures maintains cultural significance within the community. This thesis creates a hypothetical adaptive reuse plan for adapting Engel Stadium in Chattanooga, Tennessee into Engel Concourse, a gathering place for students, UTC faculty, and community …

A Metabolomics-Based Approach To The Screening Of Endometrial Cancer: Development Of A Gas Chromatography-Ion Trap Mass Spectrometry-Based Method, Allycia Lee

Honors Theses

Endometrial Cancer (EC) is the most common malignant tumor of the female reproductive tract. Currently there are no methods for effectively screening EC, but one method that is thought to be useful in screening EC is metabolomics. Metabolomics involves the analysis of metabolites which are low molecular weight inorganic and organic chemicals that are substrates, intermediates, and by-products of enzyme-mediated biochemical reactions in the cell. Previous research shows that the metabolic signature of EC patients are discernable from those of healthy patients. To aid in the development of an effective screening method for EC, blood serum, urine, and saliva samples …