Digital Commons Network^™

Applications Of Sodium Azide In The Synthesis Of Tetrazines And Hydrolysis Reactions, My Le

Honors Theses

The inverse electron demand Diels−Alder cycloadditions of heterocyclic azadienes have provided a robust methodology for synthesizing highly substituted and functionalized heterocycles. It is widely used in organic synthesis and the pharmaceutical industry in the divergent construction of screening libraries and bioorthogonal conjugation. Each heterocyclic azadiene was found to possess a unique reactivity toward different classes of dienophiles, display predictable modes of cycloaddition, and exhibit substantial substituent electronic effects impacting their intrinsic reactivity and cycloaddition regioselectivity. Synthesis of 1,2,4,5-tetrazine has been reported in the literature since the late 19th century, showing scientists' tremendous interest in its application.

Herein we attempt to …

The Effect Of Advocate Disclosure On The Perceptions Of A Young Adult Who Stutters, Madeline Simpson

Honors Theses

This between-group study designed weighed the effects of stuttering disclosure on the perceptions of a 17-year-old male who stutters, relative to perceived speech skills and personality characteristics. 1225 participants were included in the dataset analysis with a balanced distribution across all ranges. The conditions used in this study include (1) control condition (no disclosure), as well as experimental conditions including: (2) father disclosure, (3) male coach disclosure, (4) female teacher disclosure, (5) girlfriend disclosure, and (6) female speech-language pathologist. Participants in the control group only viewed the primary video stimulus, while participants in the experimental groups viewed one of the …

Effects Of Crowding Agents On I-Motif Dna, Hayden Brines

Honors Theses

Deoxyribonucleic acid (DNA) is a well-known double stranded, helical, biological molecule. In addition to its more commonly known structure, DNA can also form more complicated structures like G-quadruplexes and i-motifs (iM). The iMs are formed by cytosine rich DNA and are a four stranded structure that is typically looped around itself. The iM formation is typically pH-dependent and is favored in more acidic conditions; the pKa value is approximately 6.5. This pKa value allows for potential in vivo formation, since the cells have a pH of approximately 7.3. Due to this, iMs are thought to be powerful, innovative molecules for …

The Interhemispheric Connectivity Of The Auditory Cortices, Taylor Stansberry

Honors Theses

Objectives: The corpus callosum of the brain is an important and complex structure that allows communication between the two cerebral hemispheres. In the absence of knowledge about the relationship between the corpus callosum and the auditory cortices, this study aimed to investigate how the structure changes. Specifically, we explored the age associated changes of interhemispheric connectivity between left and right cortices through the corpus callosum.Methods: Using Diffusion Tensor Imaging (DTI) data from enhanced Nathan Kline Institute- Rockland Sample (NKI-RS), we isolated the auditory corpus callosum, or the section of the corpus callosum that transfers auditory information, in each individual subject …

Chemical And Co-Solute Effects Of Polyethylene Glycol On I-Motif Formation, Lindsey Rutherford

Honors Theses

DNA typically forms Watson and Crick double helix structures in which adenine, thymine, guanine, and cytosine pair with their complimentary DNA base. However, DNA i-motif structures can form in cytosine rich DNA, typically under slightly acidic conditions (~pH 6). DNA i-motifs are four stranded secondary structures in which cytosine pairs with cytosine to form a quadruplex. The i-motifs are typically formed in acidic conditions because of the protonation in the C•C base pair between one of the three hydrogen bases. Recent studies have suggested i-motifs can also form under neutral conditions, which is more realistic for a cell. It is …

Has Maize Overtaken Our Reality? A Personal Briefing, Biochemical Comparison, Agrigenomics, And History Of Maize, Nader Pahlevan

Honors Theses

Maize (Zea mays ssp. Mays) is a revolutionary cereal grain that has raced to the world’s most popular staple crop, transforming societies and impacting history. This paper aims to build and portray the story maize has created through its journey to world domination. The important details that encompass this literature are maize’s cultural significance in my life’s story, the comparison of various starches broken down into amylose and amylopectin ratios, a summative historical account on maize’s spread throughout numerous parts of the old world, and the genetical analysis of maize that explains the key features that have led …

Composition And Oxidative Potential Of Fine Particulate Matter From Pascagoula, Ms, Madison Smith

Honors Theses

Air pollution is one of the top-ranking risk factors for death and disability around the world, accounting for nearly 4.9 million deaths worldwide (State of Global Air, 2019). Specifically, fine particulate matter (PM_2.5) has been linked to many adverse effects on human health, yet there is very little known about what characteristics exactly cause these adverse health effects and how they cause them. Samples for this study were collected from Pascagoula, MS, a small town located on the Mississippi Gulf Coast that is home to many industrial yards, from September 2013 to December 2013. Black carbon (BC) analysis …

Bringing Biochemistry Home: Transforming Milk Into Yogurt, Ashley King

Honors Theses

Communicating the beauty and complexity of biochemistry to students in a large classroom during the pandemic: what a challenge! We undertook a novel endeavor in the Department of Chemistry and Biochemistry by introducing a mandatory kitchen chemistry experiment in a lecture course. Milk, the epitome of our identity as mammals, also contains all of the major biochemical macromolecules studied in Biochemistry I. Further, the making of yogurt invokes physical processes that are the major processes and molecular forces that dominate the content of the course. Here, we report the results of massive parallel experiment conducted in the kitchens of the …

Coronary Artery Disease: An Analysis Of Its Biochemical, Physiological, And Sociological Causes And Effects, Alexander Velazquez

Honors Theses

Coronary Artery Disease (CAD) is a serious condition caused by a buildup of plaque in your coronary arteries, the blood vessels that bring oxygen-rich blood to your heart. It disproportionately affects millions of Americans and thousands of Mississippians. This study presents the results of an evaluation of causation factors of CAD, the physiological impact on the body, and the contributing determinants of CAD on Mississippi populations. Data on Mississippi populations were collected using the Center for Population Studies at the University of Mississippi. A multitude of individuals were evaluated when examining the causes and effects of CAD including men, women, …

Exploring Mental Health & Covid-19: How A Pandemic Could Become America's Next Mental Health Crisis, Ashley D. Shofner

Honors Theses

A pandemic can be described as an epidemic disease that has spread over a large geographical area and has become prevalent in numerous sectors of the globe. In 2020, just over 100 years since our last major pandemic, the 1918 Influenza outbreak, the global community is facing yet another threat: COVID-19. While an individual’s physical health has consistently been of concern, research has failed to adequately consider the other dimensions of health. Among these additional dimensions of health is mental health and psychosocial wellbeing.

Pandemics and emerging infectious diseases have become modern influencers of mental health patterns. By analyzing the …

Investigating The Effects Of A P53 Mutation And Various Tyrosine Kinase Inhibitors On Glioma Progression And Therapy Resistance In Drosophila, Kaitlyn M. Alleman

Honors Theses

Gliomas, which are brain tumors that arise from glial cells, are some of the most aggressive and lethal types of tumors. These brain tumors are difficult to treat because not enough information regarding the mutations present in these tumors exists. This project studies effects of a p53 mutation on Drosophila glioma progression and then will test to see if this results in resistance to current chemotherapy. The main goal of this endeavor is to investigate the numerous defects occurring at the cellular and biochemical level in gliomas, which will give insight into why these types of tumors are so difficult …

The Effects Of Helicopter Parenting In Emerging Adulthood: An Investigation Of The Roles Of Involvement And Perceived Intrusiveness, Abigail T. Flower

Honors Theses

The term “helicopter parent” describes parents who provide extensive support with high constraint to their children with a variety of possible negative outcomes (Comstock, 2019). The present study examined the effects of intensive (i.e., “helicopter”) parenting among emerging college-aged adults by comparing evaluative and descriptive measures of intensive parenting and examining their differential associations with college students’ achievement and well-being. There were three main hypotheses of the study. First, I predicted that perceptions of parental intrusiveness, captured by an evaluative measure, would be more strongly correlated with negative outcomes (e.g., poorer grades, greater depression etc.) than would the frequency and …

Weird And Cosmic Short Horror Fiction And The Illustration Of Existential Fears, Brianna Williams

Honors Theses

The literary genre of horror fiction is uniquely capable of presenting its readers with the horrific, terrifying, and repulsive while remaining an acceptable and sought-after genre. Moreover, the horror genre is also often able to serve as a type of lens through which readers can recognize socio-historical and cultural fears. Within the many subgenres of horror, cosmic and weird horror continue in this ability, but are able to present their readers with more abstract, existential fears. This research is an exploration and analysis of the ability of cosmic and weird horror to reveal the real, personal existential fears of their …

Shining A Light Into The Darkness: The Developmental Impacts Of Internment In U.S. Immigration Detention Centers On Detained Latinx Children, Rebecca Messer

Honors Theses

Americans are concerned about immigration politics and how to handle the migration of immigrants into the United States, especially those from Latinx countries who immigrate illegally. In response, the United States government has formed detention centers to house the children of these illegal immigrants. While the immediate safety and developmental appropriateness of current separation practices are of concern, few have considered what the long-term developmental and transgenerational impacts on Latinx immigrant children, held within these detention centers, will be. This thesis concludes that the negative physical, emotional, and psychological impacts both in the short and long terms are expected to …

Examining The Effects Of Exogenous Polyunsaturated Fatty Acids On The Antimicrobial Activity Of Piscidins In Vibrio Cholerae, Elizabeth Allen

Honors Theses

Antimicrobial peptides are found in all organisms and have gained notoriety in recent years for their effectiveness against a variety of pathogens. Piscidins, first isolated from the mast cells of vertebrates, comprise a family of these host defense peptides. Piscidins 1 (P1) and 3 (P3), found in sea bass and other fishes, are isoforms with different modes of action. P1 induces membrane perturbation, while P3 binds DNA to exert antibacterial activity. Each has the ability to bind Cu2+ (P1Cu and P3Cu) which dramatically impacts their antimicrobial characteristics. The purpose of this study was to investigate the activity of metallated and …

Construction And Analysis Of Three Multi-Partite Synthetic Microbial Communities, Alexander J. Lazzara, Jacob K. Fanning

Honors Theses

Microbial Communities are of interest to molecular biologists hoping to understand the nature of metabolic interactions between co-existing, or possibly mutualistic, organisms. These interactions are ubiquitous in nature, but understanding the molecular mechanisms involved remains challenging and not well understood. Here, we design three tri-partite microbial circuits based on possible interactions among involved microbes, which are discussed and may suggest mutualistic interactions. Carbon and nitrogen molecular pathways and the intracellular metabolism of each microbe is discussed. We present minimal growth media that will ensure that organisms utilize available resources, which may originate from metabolic processes in neighboring microbes, simulating a …

Molecular Mechanisms Of Oxidative Stress On Molecular Motors, Addison Caspari-Linhardt

Honors Theses

Astronauts encounter many forms of ionizing radiation during space travel. Ionizing radiation has been seen to cause cancer, cataracts, radiation sickness, tissue damage, hereditary effects, and damage to the central nervous system. Ionizing radiation can damage DNA molecules through oxidation, as well as other vital proteins like molecular motors. Kinesin-1 is a molecular motor protein that essential to intracellular transport. Microtubules serve as the “roadway” for kinesin transport, where kinesin converts chemical energy from ATP hydrolysis into mechanochemical “steps” to move along the microtubules. Optical trapping has been used in previous research to measure single kinesin motor properties. We have …

Changing The Script: An Investigation Of How Gender Roles And Stereotypes Influence Women’S Career Choice In Marketing, Skylar Laine Read

Honors Theses

The purpose of this thesis is to investigate how the portrayal of gender roles and stereotypes have influenced young women growing up and how this representation has affected women’s career choices—specifically in sales. This thesis will explore the evolution of the Disney Princess, how the gender roles of these characters have influenced young girls’ perception of their role models, career choices, and perceptions of themselves in the workplace. This exploration is done through three different studies. The first is a case study of three Disney Princesses and the portrayal of women in their respective eras. The second study consists of …

An Experiment To Test For Rapid Evolution In An Introduced Ectomycorrhizal Symbiosis, Valerie Rewa

Honors Theses

The rapid evolution of introduced ectomycorrhizal-plant partnerships is an under- explored topic that may have immense impacts on ecosystems around the world. This experiment sought to identify and quantify this evolution and its impacts on both fungal colonization as well as plant growth. I used a laboratory experiment to analyze these factors in native and exotic genotypes of Suillus cothurnatus and Pinus species. Much of the data was not able to be collected, but that which was did not support the presence of rapid evolution in the mutualistic partnership. Pine species was seen to have a significant effect on plant …

Examining The Role Of The Drosophila Melanogaster Unc13 Protein In Open Field Activity Using Rnai, Islam Orabi

Honors Theses

Unc13 are proteins in the presynaptic neurons essential in controlling synaptic vesicle fusion and synaptic transmission. Recently, the reduction of Drosophila melanogaster Unc13 proteins (Dunc13), were found to result in a resistance to alcohol, highly reminiscent of tolerance formation. I investigated whether genetically reducing Dunc13 activity in Drosophila brain regions leads to different forms of behavioral plasticity using an open field activity paradigm. In my approach, a Dunc13 RNAi transgene was expressed in the Drosophila brain within the mushroom body, the ellipsoid body, and in all neurons. The activities of the flies were examined in the open field paradigm to …

The Biodiversity Of Deadwood-Associated Arthropods In The Southern Appalachian Mountains, Isabelle Ong

Honors Theses

A biodiversity hotspot is a location that has significantly elevated levels of biodiversity including many species found nowhere else, and which is also in danger of losing much of this diversity. By identifying biodiverse regions, conservation efforts can be targeted to those locations where they are likely to have the most beneficial impacts. We looked at deadwood associated arthropods within the Southern Appalachian Mountains to examine centers of biodiversity. Nine logs were sampled, three of which were located in Bankhead National Forest and six were located in the Great Smoky Mountains. Polymerase chain reaction was used to amplify the mitochondrial …

Being Female And Indigenous: Barriers To Reducing Bolivia's Maternal Mortality Rates Under Evo Morales, Channell Cole

Honors Theses

This thesis seeks to answer the question: What are the barriers to attempts to reduce Bolivia’s maternal mortality rate under Evo Morales? While Morales’ presidency began in 2006, the timeline is from 2004 to the present to account for changes due to his policy. Using activity theory and social capital theory, I argue that machismo and racism are two social factors that are barriers to efforts to reduce the maternal mortality rate. Machismo manifests itself uniquely in Bolivia, as I argue through a comparison to Paraguay. Machismo is also riddled with a history of anti-indigenous racism. I examine the Rockefeller …

Immunomodulatory Biomaterials For Cancer Immunotherapy, Larry Donnell Stokes Jr

Honors Theses

Cancer immunotherapy has become an effective treatment in the toolbox of oncologists. Immunotherapy offers a less toxic alternative to standard cancer treatments such as chemotherapy and can have prolonged curative effects to decrease cancer recurrence. Today, many drugs and biological agents have been developed that target the immune system and elicit an antitumor/cancer response. These agents are known collectively as cancer immunotherapies. While immunotherapies have radically improved treatment outcomes for many cancer patients, there are drawbacks to using these treatments. Immunotherapy treatments have poor clinical responses in patients with tumors that lack immunogenicity. Some of the treatments also pose a …

A Comparison Of Approval Ratings Of Gender Inclusive Spanish Suffixes Between Learners And Speakers Of Spanish., Chandler Nichols

Honors Theses

The following study aimed to investigate the correlation between varying levels of Spanish learners who had taken different levels of Spanish courses, and fluent speakers and their approval ratings of gender inclusive suffixes in Spanish. The study used a survey in order to gather information and approval ratings. The results indicated that higher level Spanish learners on average had lower approval ratings of the three gender inclusive suffixes included in the survey.

Perilous Place: Personal Stories Point To Possible Solutions To Widespread Flooding In The Mississippi Delta, Jared Poland

Honors Theses

The purpose of this thesis is to investigate and create journalistic stories highlighting the Yazoo Backwater Pumps Projects relationship to climate change while utilizing narrative storytelling techniques. Before explaining the methodology used for conducting research and interviews, the researcher describes the influence that innovations of mass communication channels have had on the way humans form groups and persuasively advocate for their positions. The researcher describes their historical perspective of mass media innovations that were vital considerations during their discovery and investigation of this politically divisive issue. The researcher more specifically focuses on the innovations that have occurred since the digital …

Determine The Effects Of Propionate On The Activation Of Macrophages Against The Intracellular Pathogen Listeria Monocytogenes, Leah O. Allen

Honors Theses

The main goal of this research is to see how propionate, a common food preservative and an important metabolite in humans, alters the activation of our immune system. The effects of propionate on macrophage activation will be determined by using nitrite and LDH assays. For these assays, different concentrations of propionate will be tested to determine how macrophages respond to the activation by LPS and interferon gamma. Another goal of this project is to determine the effects of propionate and macrophage activation on intracellular survival of L. monocytogenes. A gentamicin protection assay will be used to better establish the role …

Leaf Phenology And Freeze Tolerance Of The Invasive Tree Pyrus Calleryana (Rosaceae) And Potential Native Competitors, Abby E. Hay

Honors Theses

Pyrus calleryana is quickly becoming one of the most problematic invasive species in the Eastern and Central part of the United States. Pyrus calleryana is an early successional species that quickly proliferates in highly disturbed soils such as old fields and younger prairies. Currently, this species poses a large threat to land managers as it changes the successional trajectories of forest and prairie systems, creating new novel ecosystems. Pyrus calleryana outcompetes most native early successional species and is relatively understudied. The mechanism in which Pyrus calleryana utilizes to dominate ecosystems are not fully understood, but one theory is Pyrus calleryana …

Determine The Effects Of Propionate On The Interactions Between Macrophages And Listeria Monocytogenes, Stephanie M. Johnson

Honors Theses

Listeria monocytogenes is an opportunistic and intracellular foodborne pathogen that can be deadly in high risk populations. During infection in the human body, L. monocytogenes may encounter macrophages, a type of white blood cell that is critical in innate immune response both by directly targeting invading pathogens and by eliciting adaptive immune responses. During intestinal as well as peripheral infections, both L. monocytogenes and macrophages may encounter propionate, a common gut microbiome metabolite. Although propionate is shown to have various regulatory and nutritional functions, its effects on infection outcome is not well understood. Therefore, the goal of this research is …

Development Of A Computational Framework For Estimating Knee Joint Contact Forces In Running, Sean W. Kapp

Honors Theses

The prevalence of running as a form of exercise and the necessity of walking for simple locomotion obscure massive forces and moments within the body. An especial area of concern is the knee, as common among these injuries is knee pain as a result from high impact on the ground or ground reaction forces. These forces are altered by the foot strike pattern of the individual; in this study, either rearfoot strike (RFS) or forefoot strike (FFS). This alteration will impact internal forces conducted upward through the body. Given the complexity of the motion of running and the forces involved, …

An Exploration Of The Widely Observed Mechanisms Permitting Freeze Tolerance & The Potential Of Cope’S Gray Treefrog, Dryophytes Chrysoscelis, Samuel A. Ripley

Honors Theses

Over 80% of the Earth’s surface is exposed to seasonal cold temperatures less than 5.0oC. Ectotherms implement a variety of strategies to survive seasonal, or permanent, cold exposure. Some of the most common overwintering strategies are migration, hibernation, and freeze avoiding behavior. However, freeze tolerance is a minority choice among ectotherms. This strategy permits organisms to survive between 50.0 to 70.0% of their total body water volumes frozen primarily in extracellular spaces for up to several months at a time. Freeze tolerant organisms undergo minimal supercooling of their body fluids to ensure ice formation is slow and produce a wide …