Digital Commons Network^™

3d Printed Microfluidic Platforms For Elisa-Based Diagnostics: Advancing Toward Point Of Care, Brandi Marie Binkley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Protein biomarker detection is a critical analytical method for quantifying proteins in biological samples. It is relevant not only in medical diagnostics and research but also numerous other fields such as environmental monitoring, pharmacology, food safety, and biotechnology. Sensitive detection at picogram-per-milliliter (pg/mL) levels is essential for diagnosing diseases, monitoring progression, and tailoring treatments, especially for low-abundance biomarkers like troponin, interleukin 6, carcinoembryonic antigen (CEA), activin A, and vascular endothelial growth factor (VEGF). Immunoassays, particularly enzyme-linked immunosorbent assays (ELISAs), are the gold standard for protein biomarker detection due to their high specificity and sensitivity. The development of point-of-care (POC) diagnostic …

Do Dung Beetles Sink Or Swim: Exploring Dung Beetle Response To Precipitation, Lauren T. Cheshire

Graduate Theses, Dissertations, and Problem Reports (ETD)

Global climate change constitutes a major threat to biodiversity and ecosystem services of many taxa. The Mid-Atlantic region of the US is expecting to see an increase in extreme precipitation events and flooding. Dung beetles (Scarabaeinae) can tunnel below the soil surface allowing for water to dissipate into the soil rather than sit on the surface and add to flooding conditions. They also can decrease methane emission by burying dung and reduce outbreaks of pests that prefer the moist environment of dung. They contribute roughly $380 million in ecosystem services annually in the United States, yet their services may be …

What Does It All Mean? Differences In Self-Defining Memories Between Community And Adjudicated Youth, Hannah Elias

Graduate Theses, Dissertations, and Problem Reports (ETD)

Identity development is driven in large part by self-defining memories, or memories though which we base our definition of who we are (Habermas & Bluck, 2000). In adolescence, cognitive skills are gained that allow us to make meaning, or learn lessons and gain insight, from self-defining memories (Bluck & Habermas, 2001; McLean & Thorne, 2003). To determine whether there are differences in their ability to make meaning from their self-defining memories, this study compares narratives of self-defining memories between community youth and adjudicated youth. Additionally, the ability to be specific in self-defining memory recollection may differ between these groups, which …

All-Terrain Vehicle Injuries Among Youth: A Multidimensional Analysis Of Injury Patterns In West Virginia And Safety Laws Across High-Fatality States, Sabrina Chyann Beal

Graduate Theses, Dissertations, and Problem Reports (ETD)

All-terrain vehicles (ATVs) pose significant injury risks, particularly among youth. These powerful machines are widely used across recreational and rural settings. West Virginia consistently ranks among the top five states for ATV-related deaths and injuries. Safety equipment and requirements are often not followed nor enforced, causing children and adolescents to be the most vulnerable group because they tend to not wear safety equipment such as helmets, ride on roadways, carry passengers, and operate oversized ATVS. This study examined ATV injury patterns from the WVU Medicine Trauma Registry and ATV laws across the five states with the highest reported fatalities. This …

Role Of Interpretation Bias Related To Pain In A Dental Setting, Jeongwi An

Graduate Theses, Dissertations, and Problem Reports (ETD)

Studies of interpretation bias related to pain have consistently shown that patients with pain are more likely to interpret ambiguous stimuli as related to pain than healthy control participants. This phenomenon is associated with pain and related outcomes, including depression, anxiety, and avoidance behaviors, suggesting a potential target in pain management. This study aimed to examine whether the association between interpretation bias, pain, and emotional status can be replicated in a dental care population. The relation of interpretation bias to pain experience was tested in a naturalistic anxiety and pain-provoking setting (i.e., root canal treatment, RCT). A total of 82 …

Resilience In Adolescents With Type 1 Diabetes: The Relation Between Grit, Benefit-Finding, Diabetes Distress, Hba1c And Health-Related Quality Of Life, Hannah Hardiman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Type 1 diabetes (T1D) is a common pediatric chronic illness that requires complicated management by both the child and their caregiver. Research and clinical guidelines have highlighted adherence (as measured by HbA1c) and health-related quality of life (HRQOL) as important outcomes particularly in adolescents with T1D. Notably, diabetes distress has been identified as a risk factor contributing to poor adherence and HRQOL outcomes in this population. Given these considerations, understanding what resilience factors contribute to optimal outcomes is vitally important to inform future interventions. Grit and benefit-finding are two novel resilience factors that have yet to be fully evaluated …

Developing Consensus-Based Methods For The Examination And Interpretation Of Contemporary Vehicle, Architectural, And Portable Electronic Device Glasses By Micro-X-Ray Fluorescence Spectrometry, Zachary Bailey Andrews

Graduate Theses, Dissertations, and Problem Reports (ETD)

Glass is a trace material that is commonly encountered during investigations of violent crimes. When glass is recovered at crime scenes, it can be used to establish links between suspects, victims, and the scene itself. The most discriminatory form of analysis for glass evidence is elemental analysis, and micro-X-ray fluorescence spectrometry (µXRF) is becoming an increasingly common technique used for this purpose. Recent advances in µXRF technology, such as the introduction of silicon drift detectors (SDD) and improved polycapillary optics are promising in enhancing the capabilities for the examination of glass evidence in forensic investigations. However, along with these advances …

Nephrocheck Implementation And Its Impact On The Incidence Of Acute Kidney Injury After Coronary Artery Bypass Graft Surgery, Michael Maccourtney

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

NephroCheck Implementation and its Impact on the Incidence of Acute Kidney Injury After Coronary Artery Bypass Graft Surgery

Michael C. MacCourtney

Problem Statement: Acute kidney injury occurs in up to 50% of patients after cardiac surgery (Meersche et al., 2017). This contributes to significant morbidity, mortality, and costs (Helgadottir et al., 2016). At the time of the project implementation, the standard of care involved measuring renal function utilizing serum creatinine (SCr) and urine output (UOP) without NephroCheck. These renal function labs do not represent a timely and accurate assessment of the patient’s current renal status because they do not …

“We Do Everything Together!”: Examining The Effect Of A Multitasking Chore On Narrative Engagement And Parasocial Relationships With Podcast Hosts, Hailey Morgan Scherer

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation examined the link between media multitasking behaviors and the subsequent enhancement of narrative engagement and parasocial relationships with podcast hosts. Research on parasocial relationships has long established that people can develop strong pseudo relationships through aural media, such as radio and podcasts (e.g., Horton & Wohl, 1956). This has often been attributed to the charismatic nature of hosts on audio programs (e.g., Schlütz and Hedder, 2022; Perks & Turner, 2019; Heiselberg & Have 2023), however, the ability of people to listen while they engage in other tasks (e.g., driving or doing chores) can also enhance parasocial bonds. One …

No Man’S A Stone: A Qualitative Approach To Characterizing Masculinity In Older Adulthood, Montgomery T. Owsiany

Graduate Theses, Dissertations, and Problem Reports (ETD)

Suicide rates are exceedingly high in U.S. older men (aged 65 years and older). In 2020, older men were about 6.5 times more likely to die by suicide than older women and 1.14 times more likely than men aged 18 to 64 years. Masculine norms (e.g., emotional suppression, self-reliance, anger, physical toughness risk-taking behaviors, alcohol misuse) are believed to influence the presentation of mental disorders, such as depression, which has led to the conceptualization of masculine depression. Research on masculinity often focuses on the experiences of younger men, ignoring how gender identities may change as one progresses through adulthood. Meanwhile, …

Computational Approaches For Diagnosing Neurological Impairments And Restoring Functional Movement, Serhii Bahdasariants

Graduate Theses, Dissertations, and Problem Reports (ETD)

Movement is the primary means by which the nervous system converts intention into interaction with the environment. Voluntary limb motion enables standing, walking, reaching, and grasping—actions that underpin self‑care, communication, and economic participation. When diseases or injuries disrupt the pathways that couple neural commands to muscle forces—whether through cortical stroke, spinal cord injury, or peripheral nerve damage—mechanical outcomes such as weakness, loss of coordination, and ultimately reduced mobility emerge. These impairments cascade into secondary complications, increased dependence on caregivers, and diminished quality of life. Quantifying how the lesion alters the mechanics of movement is therefore essential for designing targeted interventions …

Development Of High-Performance Coaches And Burnout, Amy Cashin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Coach education is imperative, especially as coaching moves towards professionalization (Lara-Bercial, 2022). The literature has shown that high-performance coaches are closest to fitting the criteria of a profession (Lara-Bercial et al., 2022) while also being at risk of experiencing burnout due to the high level of stress faced within their job (Bentzen et al., 2014; Olusoga & Kenttä, 2016). The purpose of this study was twofold. First to have a more comprehensive understanding of Australian Athletics high-performance coaches’ wants and needs regarding coaching education as it pertains to coach wellbeing and burnout; and second, to have an in-depth exploration of …

Functionalized Carbon Nanohoops: Synthesis, Structural Insights, And Pathways To Carbon Nanobelts, Liu Li

Graduate Theses, Dissertations, and Problem Reports (ETD)

The development of structurally defined carbon nanostructures has gained significant attention due to their unique electronic, optical, and mechanical properties. Cycloparaphenylenes (CPPs), also known as carbon nanohoops, and carbon nanobelts (CNBs) represent key molecular segments of carbon nanotubes (CNTs), offering a bottom-up approach to precise nanotube construction. This dissertation focuses on the synthesis, structural characterization, and functionalization of CPPs, as well as the development of novel synthetic pathways for CNBs. A series of functionalized CPPs, including [9]-, [12]-, [15]-, and [18]CPPs, were synthesized using a modular approach involving a Diels-Alder adduct formation followed by a Ni(cod)2/bpymediated macrocyclization reaction. The influence …

Soft Epilogue, Chloe C. Firth

Graduate Theses, Dissertations, and Problem Reports (ETD)

This written document is the accompanying thesis for my Master of Fine Arts Exhibition, Soft Epilogue. The work in the exhibition serves as a visual record of my eating disorder recovery process. Each piece in the exhibition addresses a selected writing prompt from the book 8 Keys to Recovery from an Eating Disorder, published in 2011 and written by therapists and former eating disorder patients Carolyn Costin and Gwen Schubert Grabb. The book provides information and journal assignments regarding the development of eating disorders, how to maintain the motivation to recover, and how to navigate the complicated emotions …

Numerical Investigation Of Pressurized Oxy-Combustion, Lei Li

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pressurized oxy-combustion (POC) is emerging as a promising and transformative technology for carbon capture, utilization, and storage, offering advantages of low cost, low emissions, and high efficiency. POC operates by burning pulverized coal at elevated pressures in a recycled flue gas environment, typically rich in O₂ and CO₂. This study presents a comprehensive numerical investigation into the fundamental behaviors of lab-scale POC reactors, using both two-dimensional (2D) Reynolds-Averaged Navier-Stokes (RANS) simulations and three-dimensional (3D) Large-Eddy Simulations (LES) with the commercial computational fluidized dynamics (CFD) software package ANSYS Fluent.

The primary focus of this work is to understand flame stabilization and …

A Limb-Speed-Driven Locomotor Control System And Its Ability To Adapt, Emily Marie Herrick

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite how simple walking may seem, the locomotor control system is structurally and functionally complex. Its hierarchical organization of supraspinal and spinal networks with forward and feedback pathways has many interactions at multiple levels that are dependent on the dynamics of a high-dimensional musculoskeletal system. Having a comprehensive understanding of sensorimotor integration within a healthy locomotor control system is crucial for understanding changes to the system due to age or neurologic disease and developing effective technologies to recover mobility in those populations. In this dissertation, we address persistent gaps in knowledge pertaining to how the nervous system controls locomotion.

In …

Apostrophes And Archimedes, Evan J. Clough

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis explores how digital collage and printmaking can be used to develop images through acts of subjective response, intentional and unintentional acts of appropriation, and parodic re-use and comparison pushed to absurd extremes. It looks at the history of creating art for parodic re-use and examines “Pataphysics”, developed by and for art appropriation of pattern, form, and process. These concepts are explained and related to visual artwork that was made for the thesis exhibition Apostrophes and Archimedes Cast Shadow Puppets. The exhibition invites viewers to experience the phenomenon of observation, automatic transformation, and creation using multiple modalities of exaggerated …

Efficacy Of Salmonella Surrogate Enterococcus Faecium In Poultry And Mash Broiler Feed, Corey Waitman Coe

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation investigates the efficacy of Enterococcus faecium as a surrogate for Salmonella in validating thermal inactivation processes during poultry feed manufacturing and meat processing. The research encompassed three studies evaluating microbial kinetics in mash broiler feed, reconstructed ground chicken, and predictive modeling approaches to improve food safety interventions. In the first study, the thermal resistance of nalidixic acid-resistant Salmonella Typhimurium and E. faecium was compared in mash broiler feed heated at 75–95°C. Results demonstrated that E. faecium exhibited significantly greater heat resistance than Salmonella, with longer shoulder times, greater tailing effects, and higher D-values, supporting its use as …

The Economics Of Crime And Reform: Essays On Alcohol Taxation, Cannabis Legalization, And Felon Enfranchisement., Georgios Marios Bantis

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation comprises three independent yet thematically connected essays that explore the effects of public policy on crime and municipal finance. The first chapter examines the relationship between alcohol taxation and crime, assessing how changes in excise tax policies influence criminal activity. The second chapter investigates the fiscal implications of felon enfranchisement, analyzing how the restoration of voting rights to individuals with felony convictions affects municipal public finance. The third chapter evaluates the impact of cannabis legalization on crime, focusing on whether and how legalization alters crime patterns. Each chapter is structured as a stand-alone research paper. A general introduction …

Social Support And Negative Affective Outcomes Among Older Adults: Does Valuing Autonomy Matter?, Erika Anne Fenstermacher

Graduate Theses, Dissertations, and Problem Reports (ETD)

While not a normal part of aging, depression is a concerning psychiatric condition in late life. Depression is a significant predictor of suicide and the highest rates of suicide occur in middle aged and older adult men. One factor that may protect against depression and suicidal behavior among older adults is social support. Social support has been demonstrated as a significant factor in health and well-being. Perceived social support has consistently been linked to beneficial outcomes, however, received support has mixed results with some studies even finding received support to be harmful. For individuals who highly value autonomy, it may …

Customer Segmentation And Fuel Economy Prediction Using Telemetry Data From Heavy-Duty Trucks, Batishahe Selimi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Heavy-duty trucks constitute only a modest fraction of on-road vehicles, yet their intensive duty cycles and high fuel demands yield a disproportionately large share of transportation fuel use and greenhouse gas emissions. Addressing this imbalance requires data-driven tools that capture the realities of fleet operation and translate complex telemetry into actionable insight.

This dissertation introduces a unified machine-learning framework that operates exclusively on high resolution time-series data collected from fifty-nine diesel trucks deployed across Southern California. It begins by constructing a multi-modal feature space that blends statistical summaries of key engine signals, static vehicle descriptors, and Mel-Frequency Cepstral Coefficients, thereby …

Framing The Moment, Shaping The Movement: News Narratives And Public Response To George Floyd’S Death, Madison B. Hall

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study investigates how national and local U.S. news outlets framed George Floyd, Black Lives Matter protesters, and the surrounding protests in the immediate aftermath of Floyd’s death. Through a qualitative content analysis of YouTube news broadcasts posted between May and June 2020, coupled with thematic analysis of popular comments that viewers posted in response, this research explores media narratives and public understanding of a pivotal moment of an ongoing racial justice movement. Drawing on framing theory and construal level theory, the study identifies a range of frames (i.e., pro-accountability, anti-accountability, raw perspective, neutral, episodic, riot) used by both local …

Long-Term Trends In Brook Trout Production And Large Wood In West Virginia Headwater Streams, Halden D. Edwards

Graduate Theses, Dissertations, and Problem Reports (ETD)

Projects which monitor long-term trends in ecosystems are uncommon but extremely important in the management of fish and wildlife populations. By assessing the health of a population or community over multiple temporal scales, these projects provide true insight into population success that cannot be elucidated through short-term studies. Long-term projects are also instrumental in assessing the effects of disturbance events as they provide valuable baseline information which can be compared to data collected post-disturbance. Even more importantly, long-term studies can help identify crucial changes in the health and success of imperiled and invaluable species to improve and focus conservation efforts. …

Artificial Intelligence Approaches For Membrane Fouling Prediction, Yazhou Jiang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Membrane fouling is a persistent and critical challenge in membrane-based separation processes, resulting in reduced performance, higher energy consumption, and increased operational and maintenance costs. Addressing this issue is essential for improving the long-term sustainability and efficiency of membrane systems. This report provides a comprehensive overview of membrane fouling mechanisms—such as organic, inorganic, particulate, and biofouling—and explores the growing role of Artificial Intelligence (AI) in tackling this problem. Specifically, it reviews three widely adopted AI techniques: Artificial Neural Networks (ANN), Fuzzy Logic (FL), and Support Vector Machines (SVM). Each method is examined through a series of literature-based case studies, …

Finite Element Modeling And Experimental Characterization Of Overmolded Gold-Palladium Composite Leads And Their Mechanical Behavior Under Cyclic Fatigue For Long-Term Implant Applications, Tanner Davis

Graduate Theses, Dissertations, and Problem Reports (ETD)

Auditory Nerve Interface (ANI) devices, currently in developmental stages, represent an emerging neuroprosthetic approach to restore hearing in severe sensorineural loss patients by directly stimulating the auditory nerve via the Utah Slanted Electrode Array (USEA). Conventional implant leads are comprised of multiple wires made from materials such as platinum-iridium and MP35N stainless steel that are overmolded in an insulative material. These materials are not possible to bond with to electrode array used in the ANI device, necessitating the evaluation of a new material like Au-Pd to ensure its functionality and durability under cyclic stresses. This gap necessitates characterization of alternative …

Reactive Model-Free Control For Underactuated Vehicles Operating In Resource-Constrained Environments, Austin Lane Sponaugle

Graduate Theses, Dissertations, and Problem Reports (ETD)

Buoyancy-driven, underactuated vehicles offer a compelling solution for long-duration autonomous operations in constrained environments. By leveraging buoyancy to generate lift, these vehicles can remain aloft for extended periods with minimal power requirements, making them well-suited for applications in atmospheric monitoring, planetary exploration, and communications. However, their limited actuation authority, strong coupling with environmental forces, and operating domains introduce significant control challenges, including nonlinearity, sensitivity to external disturbances, and limited observability.

This work investigates the design and implementation of lightweight, reactive model-free control strategies that enable robust autonomy in such constrained settings. A digital Extremum Seeking Controller (ESC) is first implemented …

Dynamically Scaling Biomimetic Robots Through Parallel Viscoelastic Actuators, Foster Olaf Holmquist

Graduate Theses, Dissertations, and Problem Reports (ETD)

Robots provide a unique proving ground for testing scientists’ understanding of neuromuscular systems. Synergistically, mimicking biological control laws and mechanics could offer robotics the robust and adaptive locomotion of animals. Previous work has utilized the wealth of research on insect biomechanics to develop scaled robotic models but doing so presented a problem: while these robots’ larger scale has simplified manufacturing, the scale has also shifted its mechanics from those of the modeled organisms. While animals walk, forces are generated that describe their mechanics and influence motor control. The musculoskeletal structure and stretch of muscles result in viscous and elastic forces, …

The Effect Of Cerebellar Feedback On The Spatial Processing Of Conspecific Signals In The Electrosensory System, Dakota Ray Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

Animals have evolved sensory systems to help decipher important environmental signals that are vital for their survival. Feedback pathways are necessary to allow these signals to stand out in a noisy background, enabling the animal to focus on them more effectively. The electrosensory system of Apteronotus leptorhynchus allows the fish to sense the modulations caused by other conspecifics to allow for proper responses during social interactions. Electroreceptors detect these EODs, and afferent nerves project these signals to the ELL, where electrosensory information is projected to other parts of the brain. Cerebellar feedback to the ELL allows the suppression of lower …

Molecular Characterization Of Fungus-Arthropod Associations, Angie M. Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Arthropods, or phylum Arthropoda, are the largest group of animals, accounting for over three quarters of all animal records on NCBI Taxonomy. And yet, only 40% of all deposited sequences on NCBI Nucleotide come from arthropods. Clearly, arthropods are an understudied group of animals: they are small, often drab, and lead lives very alien to our own. Arthropods have chitinous exoskeletons, segmented bodies, and jointed appendages, and include crabs, barnacles, insects, spiders, and millipedes. Since the era of DNA based studies took off in the 1980s, DNA and other small molecules have been integral to our understanding of the Earth …

A Quality Improvement Initiative To Decrease Sugammadex Expenditures, Jordan Austin Gardo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

A Quality Improvement Initiative to Decrease Sugammadex Expenditures

Jordan Gardo

Introduction/Background: The manufacturer’s suggestion in the dosing of the Sugammadex is

generally dosed at 2-4 mg/kg of total body weight (TBW) depending on the extent of

neuromuscular blockade. Evidence suggests that in the morbidly obese population, this

can lead to excessive dosing due to the drug's hydrophilic properties, making it less cost-

effective. Dosing based on adjusted body weight (ABW) leads to dose reductions while

achieving full reversal of neuromuscular blockade as measured by quantitative train-of-

four monitoring, resulting in lower costs from decreased vial usage.

Purpose: The purpose …