Digital Commons Network^™

Health Disparities By Sexual Orientation Components In The United States, Julia Kay Wolf

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fundamental cause theory encourages researchers to consider broad social conditions that put people at risk of individual-level health-related risks that can lead to health disparities between social groups. Stigma has recently been proposed as a fundamental cause of health disparities as it influences multiple disease outcomes, affects access to resources, and is consistently related to health inequities across historical and geographical contexts. Minority stress theory describes how sexual minorities endure excess stressors in the form of prejudice and discrimination due to their stigmatized status. Considering both frameworks, I explore how stigmatized sexual orientation minority respondents compare to their sexual majority …

Enabling Technologies For Medicinal Chemistry And Synthesis: I. Cannabinoids; Ii. Illudalic Acid; Iii. Microwave Chemistry, Harvey Fulo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Innovations in synthetic organic chemistry such as methodology improvements, new chemical transformations and technology development significantly impact medicinal chemistry and continue to drive the drug discovery process. Here, new cyclization strategies to the pharmaceutically underexplored 3,3-dimethylcyclopentane-fused aromatic rings are described. One approach highlighted oxidative cycloisomerization, intermolecular [2+2+2] cyclotrimerization and 5-exo-dig cyclization providing indanoylindole cannabinoids that possess high affinity for human CB₂ receptors at nanomolar concentration and good selectivity index. Another featured a [4+2]-type benzannulation reaction which enabled the 5-step synthesis of illudalic acid — compared to the current available sequences (≥16 steps) — and its equipotent and LAR-selective analogs. …

Investigation Of Novel Mitoneet Ligand Nl-1 As A Therapeutic For Cerebral Ischemia And Reperfusion Injury, Pushkar Saralkar

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cerebral ischemia reperfusion injury, a common type of stroke, is a neurodegenerative disorder which is the leading cause of permanent disability in the United States. The underlying pathophysiology of ischemic stroke involves an occlusion in the vasculature supplying oxygen and glucose to the brain tissue, leading to infarction and eventual necrosis in the brain. MitoNEET is an outer mitochondrial protein that plays a role in the cellular bioenergetics and its redox physiology. Although its endogenous ligands are under investigation, mitoNEET binds molecules with a thiazolidinedione moiety, such as NL-1. This dissertation investigates NL-1 as a potential cytoprotective agent, and its …

Analysis Of Cell Signaling Perturbations In Response To Chronic Localized Infections, Amber Nicole Prince

Graduate Theses, Dissertations, and Problem Reports (ETD)

The tissue-level response to pathogens involves an intricate series of signal transduction events, influenced by immune and healing mediators that alert the host to danger and eliminate the infection. Disruptions to normal signaling events can compromise the host’s ability to respond and lead to the development of chronic infections that cannot be resolved without clinical intervention. Prolonged inflammation due to chronic infection can damage tissues and compromise healing processes, thus, the interactions of immune and healing mediators in signaling cascades are intimately linked to tissue health outcomes. Studying signaling networks relevant to these responses provided a more thorough understanding of …

Self-Efficacy, Trait Mindfulness, And Self-Compassion As Predictors Of Risk Of Substance Use Relapse, Jonathan Nauser

Graduate Theses, Dissertations, and Problem Reports (ETD)

Previous research has demonstrated relationships among mindfulness, self-efficacy, and substance use relapse. Contrasting research has found mindfulness and self-efficacy are not consistently negatively associated with substance use relapse demonstrating a need for further research. An additional factor that has been found to associated with substance use is self-compassion; however, it has yet to be examined in relation to the process of relapse. The current study assessed self-efficacy, self-compassion, and mindfulness and their associations with substance use relapse, with the aim of predicting relapse risk. Higher levels of relapse risk were found to be negatively associated with self-efficacy, trait mindfulness, and …

Evaluating Avian Occupancy On Sites Treated With Nrcs Conservation Practices Implemented To Benefit Cerulean (Setophaga Cerulea) And Golden-Winged Warblers (Vermivora Chrysoptera) In West Virginia, Lincoln R. Oliver

Graduate Theses, Dissertations, and Problem Reports (ETD)

The USDA - Natural Resources Conservation Service (NRCS) supports private lands conservation across the United States to benefit imperiled focal wildlife species using conservation practices to restore habitat. Through the Cerulean Warbler (Setophaga cerulea) Forestland Enhancement Project (CWAFEP) and the Working Lands for Wildlife (WLFW) Golden-winged Warbler (Vermivora chrysoptera) initiative, the NRCS provides technical and financial assistance to private landowners to implement conservation practices that follow science-based habitat management guidelines. Few research studies have evaluated focal species-specific occupancy on CWAFEP and/or WLFW sites, and research is required to inform each conservation project’s effectiveness in an adaptive …

Unconventional Gas Pipeline Right-Of-Way Influence On Wildlife, Samuel C. Knopka

Graduate Theses, Dissertations, and Problem Reports (ETD)

The development of horizontal drilling for natural gas in the Marcellus Shale in 2004 resulted in rapid exploitation of the play in the Appalachian Basin. This “unconventional” gas well drilling accesses resources deeper beneath the surface and allows for multiple wells to be co-located on a single well pad. This results in fewer, but larger, well pads on the landscape than traditional vertical wells. Pipelines are required to transport the volumes of gas produced by unconventional wells to production facilities and market. The effects of gathering pipelines, which transport gas from well pads to larger transport pipelines, are poorly studied …

Effect Of Diet, Age, And Genetics On Rainbow Trout Growth And Fillet Quality, Bahareh Adami

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this study, analyses were conducted to compare fillet quality attributes of 4 rainbow trout families (40, 102, 159, and 187) fed fish meal (FM) and a fishmeal free diet (FMF), to two age endpoints, 9 and 11 months.

Previous studies with rainbow trout have evaluated the effect of age, diet, and family on growth performance and fillet quality. However, a comprehensive analysis of variables is lacking for rainbow trout, one of the main aquatic foods for domestic and international consumers.

Separable muscle as a percent whole body weight was not affected by diet, family, age, and the interaction of …

Climatic Drivers Of Growth In Mixed Conifer Forests Of The Sierra Nevada For Different Tree Sizes And Thinning Treatments, Andrew M. Hirsch

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Mediterranean region of northern California is projected to get increasingly warmer under all Intergovernmental Panel on Climate Change (IPCC) emission scenarios, with future precipitation projections not showing much of a trend. This poses a problem to the already dry summers that are experienced in the Mediterranean region of California. If precipitation does not increase alongside temperatures, the dry seasons will likely only get drier. The use of dendroclimatology to assess how mixed conifer species in the Sierra Nevada responded to past climate is a key resource that can be used to infer how trees may respond to a future …

Adolescents' Production Of Clausal Structures In Written Narrative, Expository, And Persuasive Genres, Lauren Elizabeth Myers

Graduate Theses, Dissertations, and Problem Reports (ETD)

The aim of this study was to analyze written language samples across different genres and age groups to provide information about the production of complex syntax emergence as time progresses across tasks. Complex syntax is both a socially and academically relevant language skill that is observed to advance well into later life. In order to analyze language samples as a way to identify language abilities, we must first understand syntactic emergence across genres with adolescents who are identified as having typical language development. In this study, written narrative, expository, and persuasive language samples were elicited from sixty typical adolescents in …

Fundamentals And Applications Of Charge Transfer Dissociation Mass Spectrometry, Zachary Joseph Sasiene

Graduate Theses, Dissertations, and Problem Reports (ETD)

The recent surge in the analysis of biomolecules has driven the need for robust, versatile, sensitive, and accurate techniques such as tandem mass spectrometry for compound structural elucidation. Many fragmentation techniques have been developed over the years, but each has their own limitations, such as the loss of post-translational modifications (PTMs), inability to fragment singly charged precursor ions or cost prohibitiveness. To help fill this gap, a novel fragmentation technique called charge transfer dissociation (CTD) has been developed to fragment stored precursor ions through radical-driven fragmentation processes using kiloelectronvolt reagent cations. CTD has shown success in the analysis of proteins, …

Describing A Putative Corollary Discharge Circuit In Drosophila, Kaitlyn Nicole Boone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Corollary discharge (CD) circuits provide critical information about movement and behavior to provide context to sensory processing. However, to date, there has not been a comprehensive study of CD circuits at a single-cell level. In this thesis, I aimed to resolve the connectivity of ascending histaminergic neurons, consisting of two pairs, the mesothoracic pair (MsAHNs) and metathoracic pair (MtAHNs) at a single-cell level and characterize contexts of activation. Using transgenic techniques, connectomics and transcriptomics, we identify neural populations receiving input from the AHNs and neural populations with significant output to the AHNs. We explored where the AHNs predominantly receive synaptic …

Ecology Of Freshwater Turtles And Other Wetland Wildlife In A North-Central West Virginia Watershed, Darien N. Lozon

Graduate Theses, Dissertations, and Problem Reports (ETD)

The goal of wetland mitigation in the United States is to achieve a no-net-loss of wetlands; however, mitigated wetlands must be monitored to ensure wetland function is comparable to natural wetlands. In this study, relationships between land use practices and freshwater turtle abundance, wetland connectivity, and heavy metal bioaccumulation were investigated. In Chapter 1, a summary of pre-restoration species abundance and diversity is provided for anurans, birds, benthic macroinvertebrates, fishes, small mammals, plants, and turtles in riparian wetlands along Ruby Run, a tributary of Deckers Creek in north-central West Virginia, USA. These data provide a baseline for comparison after mitigation …

Exploring The Perceptions Of Produce Processors Operating In Non-Profit Commercial Kitchens In West Virginia, Megan Christine Govindan

Graduate Theses, Dissertations, and Problem Reports (ETD)

According to the Centers for Disease Control and Prevention, 48 million people get sick, 128,000 are hospitalized and 3,000 die each year from foodborne illness. The Food Safety Modernization Act (FSMA) shifted the focus of the Food and Drug Administration from response, to prevention of foodborne illness. The FSMA identified seven rules related to food safety prevention measures, including inspection and compliance, response and enhanced partnerships to ensure food safety along the food system, and employee-training compliance measures. Increasing access to healthy, local foods has economic, public health, and environmental benefits. Farm to Institution policies are becoming more popular nationally, …

Observational Measurement Of Attachment In Toddlers With Disruptive Behavior Using The Strange Situation Procedure And Attachment Q-Set, Corey C. Lieneman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Child-caregiver attachment is an important factor in healthy child development and is often targeted by early intervention programs. To assess the efficacy of these interventions, attachment must be accurately measured across the toddler years in populations referred for treatment of externalizing behavior problems. The Strange Situation Procedure (SSP; Ainsworth et al., 1978) and Attachment Q-Set (AQS; Waters, 1987) are empirically-validated measures of child-caregiver attachment, each with unique strengths and weaknesses. Previous research has reached mixed conclusions on relations between the observer-report AQS and SSP, depending on sample and study characteristics, and a review of the literature did not produce any …

Mitochondrial Aspects Of Neuronal Pathology In Triple-Transgenic Alzheimer’S Disease Mice, John Zachary Cavendish

Graduate Theses, Dissertations, and Problem Reports (ETD)

Alzheimer’s disease (AD) is a fatal, progressive neurodegenerative disease afflicting millions of people in the United States alone and is the only one of the top leading causes of morbidity and mortality with no effective disease-modifying therapies. It is the most common form of dementia, affecting one in three people over the age of 85. While the hallmarks of the disease include accumulation of beta-amyloid-based extracellular plaques and hyperphosphorylated tau-based intracellular neurofibrillary tangles, treatment strategies centered on removing or mitigating these components of AD have all failed in humans. Mitochondrial dysfunction has been increasingly recognized as an early and consistent …

Bypassing The Blood-Brain Barrier: A Physical And Pharmacological Approach For The Treatment Of Metastatic Brain Tumors, Samuel A. Sprowls

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation (a) provided an in depth literature review of methods to disrupt the BBB/BTB and improve therapeutic distribution to brain tumors, (b) evaluated the use of azacitidine as a single agent therapy for the treatment of brain metastasis of breast cancer and a potential molecular mechanism by which brain tropic cells are sensitized to hypomethylating agents, (c) determined the impact cannabidiol has on P-glycoprotein mediated efflux at the blood-brain barrier and its potential for use as a single agent treatment for metastatic brain tumors, (d) developed a preclinical radiation therapy protocol for use in small animals and in vitro …

Evaluation Of Population Structure, Age, Growth, And Mortality Of Blue Catfish And Flathead Catfish Within The Robert C. Byrd Pool Of The Ohio And Kanawha Rivers, Joseph Vincent Siegel

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Ohio River is continuing to recover from a long history of anthropogenic impacts. Blue Catfish were considered locally extirpated from the West Virginia sections and have been reintroduced through fingerling stockings beginning in 2002. Flathead Catfish are naturally sustaining in the pool and combined with Blue Catfish provide two popular recreational fisheries. The Blue Catfish and Flathead Catfish studies shared similar objectives: (1) evaluating size structure, (2) estimating growth rates, and (3) estimating annual mortality. From 2017 to 2020, we collected catfish annually through low frequency electrofishing and trotline surveys at 10 fixed locations. Electrofishing catch rates for Blue …

Tree Species Influence On Microbial Functional Gene Abundance And N Cycling In Riparian Soil, Elizabeth Matejczyk

Graduate Theses, Dissertations, and Problem Reports (ETD)

Little is known about how above-ground vegetation may differentially influence the below-ground microbial community structure, abundance, and function. Abundance of soil microbial N cycling genes responsible for nitrification (amoA), denitrification (nirK and nirS), and nitrous oxide reduction (nosZ) may vary with tree species and increasing N availability, and these variables may be used to predict production of NO₃^- and N₂O from soil. Variability of nitrification and denitrification rates have also been linked to tree mycorrhizal associations, with soil beneath tree species associated with arbuscular mycorrhizal (AM) exhibiting greater denitrification rates …

Soil Microbial Nitrogen-Transforming Genes Influenced By Tree Species And Fertilization In An Appalachian Hardwood Forest, Emma G. Fox-Fogle

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many forest ecosystems have been receiving elevated nitrogen (N) deposition due to human activity. Increased ecosystem N threatens soil fertility, water and air quality, and modifies the soil microbially-mediated N cycle in complex ways. The current study investigates how the abundance of key functional N cycling genes within soil bacteria is altered by N deposition and further mediated by different dominant tree species at the Fernow Experimental Forest, WV. Soils were analyzed from two watersheds, where watershed 3 (WS 3) has been receiving experimental applications of N fertilizer that are 2x historic ambient N deposition and 5x the current ambient …

Effect Of Fecal Egg Count Estimated Breeding Value On Antibody Production In Katahdin Lambs, Kelsey L. Bentley

Graduate Theses, Dissertations, and Problem Reports (ETD)

With current pressures to seek out genetic solutions to combat antimicrobial resistance, genetic resistance to the gastrointestinal nematode Haemonchus contortus may reduce need for prophylactic antibiotic use. The Katahdin breed has effectively utilized post-weaning fecal egg count estimated breeding value (PFEC EBV) to reduce parasitism within their flocks, but there still remains significant opportunity for selection within breed. In a previous study, Katahdin sheep sired by low PFEC sires had greater survivability to weaning than lambs sired by high PFEC rams and preweaning was caused by other diseases than parasitism. Those data lead to the hypothesis that selection for PFEC …

Employing Exoglycosidase And Transferase Enzymes In Capillary Nanogel Electrophoresis For The Determination Of N-Glycan Linkages And Enzyme Michaelis-Menten Constants., Lloyd Bwanali

Graduate Theses, Dissertations, and Problem Reports (ETD)

As a post translational modification protein glycosylation plays a crucial role in protein signaling, binding, kinetics and folding. In disease diagnosis and prognosis, monitoring glycosylation has been identified as a biomarker. Sialylation and sialic acid linkage in N-glycans are markers of cancers including liver, pancreatic and kidney cancer. Quantification of sialic acid linkage is analytically challenging because of the diverse linkages and the presence of heterogenous branching. A capillary electrophoresis method is reported that integrates a unique combination of enzymes and lectins to modify sialylated asparagine-linked glycans (N-glycans) in real time in the capillary so that N-glycan structures containing α2–6-linked …

Women, Water, And Well-Being: Gendered Experiences Of Household Water Contamination In Parkersburg, West Virginia, Emily Brooke Tingler

Graduate Theses, Dissertations, and Problem Reports (ETD)

DuPont Washington Works chemical plant knowingly pumped hundreds of thousands of pounds of toxic C8 waste into local waterways, water tables, and landfills for over 40 years, contaminating the drinking water for more than 100,000 residents in and around Parkersburg, West Virginia. Drawing on feminist political ecology and the political ecology of water with literature on perceptions of risk in contaminated communities, environmental reproductive justice, modern water, and hydrosocial waterscapes, I examine, through a qualitative case study, the lived experiences of women who have an intimate understanding of C8 water contamination from the DuPont Washington Works chemical plant. Specifically, I …

Independent, Semi-Automated Classification Of Petrographic Features In Volcanic Rocks Using Fiji And Weka, Holly Danielle Pettus

Graduate Theses, Dissertations, and Problem Reports (ETD)

Traditional methods of collecting quantitative petrographic data from thin sections (modal mineralogy, size distribution, shapes, etc.) are time- and labor-intensive, and rarely have sample sizes adequate to statistically describe complex rocks (i.e. volcanic rocks). Although manual counting and measurements are now routinely supplemented by digital image analysis, the majority of quantitative petrographic studies still go through a manual digitization stage where object classes are traced before further analyses. This is a major rate-limiting step that reproduces the same problems of small n-values resulting from significant effort. We have valuated the potential and limitations of using the Trainable Weka Segmentation (TWS) …

The Effects Of Genetic Differences In An Innate Immune Receptor On Immune Responses To Haemonchus Contortus In Sheep, Denzel Deshawn Middleton

Graduate Theses, Dissertations, and Problem Reports (ETD)

Little is known about differences observed in early immunological signaling events that result in cellular infiltration and Th2 cytokine production that mediate parasite expulsion. Parasite resistant St. Croix (STC) sheep have been used as model for full host protective immunity as they present the ability to respond to larval stages of H. contortus. RNA sequencing analysis of peripheral blood mononuclear cells (PBMC) stimulated with H. contortus larval antigen (HcLA) revealed significant upregulation in NLRP3 and its pathway component TLR4. Predicted protein structural software identified distinct differences in NLRP3 protein structure in STC and parasite susceptible Suffolk (SUF) sheep. NLRP3 …

Exploring Early Signaling Associated With Complex Stressors, Julia A. Penatzer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Humans are constantly exposed to a vast number of stressors in our everyday lives from social interactions to physical activity to chemical exposures, to name a few. These stressors have significant impacts on an individual, from the cellular to whole organismal level. The body contains an intricate communication system that elicits a number of both biological and physiological responses as it attempts to maintain homeostasis. Observing these responses to stressors, can offer insight into a number of outcomes, which can be beneficial (increasing life longevity) or detrimental (leading to a disease state). By measuring specific biological adaptations at particular time …

Grouping Algorithms For Informative Array Testing In Disease Surveillance, David Sokolov

Graduate Theses, Dissertations, and Problem Reports (ETD)

In order to maintain normal operations and prevent unnecessary morbidity and mortality during times of disease outbreak, institutions find a need to conduct frequent and widespread testing of their constituents, often under significantly limited testing resource constraints. Faced with the challenge of how best to allo- cate these limited resources to maximum effect, institutions are increasingly turning to group (or “pooled”) testing, which involves testing strategically-chosen groups of patient samples rather than individual samples, producing significant testing resource savings under certain regimes of disease prevalence. While group test- ing can be conducted without any a priori knowledge of individual disease …

Biocompatible And Multifunctional Trityl Spin Probes For Electron Paramagnetic Resonance Spectroscopy, Teresa D. Gluth

Graduate Theses, Dissertations, and Problem Reports (ETD)

The primary objective of my thesis was to develop and utilize a biocompatible multifunctional trityl spin probe for concurrent measurement of pO₂, pH_e, and [P_i] in vivo by electron paramagnetic resonance (EPR) spectroscopy (Chapter 2). My first goal was to synthesize the proposed probe we are terming HOPE71. Secondly, HOPE71 was characterized by X-band and L-band EPR spectroscopy. Next, the biocompatibility of HOPE71 was assessed through an albumin binding test, cytotoxicity assays, and in vivo intravenous tolerance. Then, the use of HOPE71 to measure the target parameters was demonstrated in a breast cancer …

Huntingtin Aggregation At Interfaces Associated With Membranes And Organelles, Adewale Vincent Adegbuyiro

Graduate Theses, Dissertations, and Problem Reports (ETD)

Huntington’s Disease (HD) is a genetic neurodegenerative disease caused by the expansion of polyglutamine (polyQ) domain within the first exon (exon1) of the huntingtin (htt) protein. Due to this mutation within the polyQ domain, htt aggregates into various toxic species such as oligomers, fibrils, and other amorphous aggregates. While the aggregation of htt strongly correlates with polyQ length, other factors, e.g. interaction with membranes or organelles and posttranslational modifications (PTMs), modulate aggregation. The first 17 N-terminal amino acids (Nt17) that precede the polyQ in htt-exon1 enhances aggregation and facilitated binding of htt to membranous organelles, promoting morphological changes and disfunction. …

The Effects Of Dementia Knowledge On Dementia Worry, Attitudes, Social Comfort, Empathic Concern, And Personal Distress, Alexandria Rose Ebert

Graduate Theses, Dissertations, and Problem Reports (ETD)

The present study examined the effects of different types of dementia knowledge on attitudes and affective reactions towards persons with dementia. Work has demonstrated that individuals with higher levels of personhood-based knowledge have lower levels of personal dementia fear and higher levels of social comfort. However, to our knowledge, work has not examined dementia attitudes more broadly or differentiated causal relations among different forms of dementia knowledge and attitude outcomes. Participants (N = 334) aged 19-78 (M = 44.53, SD = 16.57) were randomized into one of five experimental conditions: (1) biomedical-knowledge (BK; read biological and medical facts about …