Digital Commons Network^™

Discriminative Stimulus Effects Of Cannabidiol Oil In Rats, Rebecca L. Chalme

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cannabidiol (CBD) is one of the major centrally active phytocannabinoid components of cannabis, and has been approved by the FDA only for the treatment of two rare seizure disorders. However, CBD has been touted as a potential treatment for anxiety in place of more traditional treatments like benzodiazepines. Although there is some evidence of anxiolytic effects of CBD, its suitability as a substitute for benzodiazepines is unknown. This experiment was designed to assess to what extent CBD shares interoceptive discriminative-stimulus properties with the anxiolytic chlordiazepoxide (CDP), a benzodiazepine. In the present experiment, a range of doses (0-333.3 mg/kg) of over-the-counter …

Evaluation Of Dental And Skeletal Changes With Sequential Distalization Of Maxillary Molars Using Clear Aligners: A Preliminary Study, Minh Phi Nguyen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Class II malocclusions can be treated with appliances such as the pendulum appliance or distal jets to distalize maxillary molars to achieve a Class I molar relationships. It is not clear in the literature whether these clear aligners can distalize maxillary molars, dental tipping, or translation. The objective was to evaluate the dental and skeletal changes in three planes spaces using CBCT scans and compare the treatment changes with a control group of subjects. Methods: Eight Class II division 1 malocclusions patients treated with maxillary molar distalization using clear aligners. Twelve molars were evaluated for tooth movement in three …

A One-Session, Brief Acceptance And Commitment Therapy Workshop For Chronic Pain Patients: A One-Sample Pretest-Posttest Prospective Exploratory Study, Kelly Anne Thomas Ms

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chronic pain affects one in every four persons (NIH, 2010). For individuals residing in rural communities where chronic pain treatment is often not accessible (Artnak et al., 2011), a one-session brief mental health intervention is a critical healthcare need. More specifically, acceptance and commitment therapy (ACT) for chronic pain is a novel treatment approach in need of more research (Society of Clinical Psychology, 2016). This current study contributes to the gap in the literature by implementing an ACT workshop in a rural healthcare setting for individuals with chronic pain. It was hypothesized that the workshop would increase adaptive coping mechanisms …

Accuracy Of Digital Impressions At Varying Implant Depths: An In-Vitro Study, Vivia Sequeira

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Implants placed at variable depths may vary the amount of visible scannable surface of a scan body. Intraoral scanner technology uses advanced optical principles to record the surface of the scan body to accurately capture the implant position.

Purpose: To investigate the effect implant placement depth has on the accuracy of digital implant impressions using an intraoral scanner.

Materials & Methods: A partially edentulous gypsum master model was fabricated to allow the positioning of a single implant analog at different depths. Four groups based on the planned implant depths of 0, 3, 6 and 7mm and corresponding visibility of …

Valorization Of Xylan In Agroforestry Waste Streams, Harrison Appiah

Graduate Theses, Dissertations, and Problem Reports (ETD)

Valorization of Xylan in Agroforestry Waste Streams.

Harrison Appiah

Microwave-assisted deep eutectic solvent and gamma-valerolactone metallic chloride catalyzed conversion of xylan to furfural were investigated using a 2x3 factorial experimental design at two levels of percent microwave power, reaction time, and catalyst concentration. The levels of each factor studied were (20%, 60% microwave power, 2, 4 minutes, and 10, 20mg) respectively. The effect of three metallic chloride catalysts (LiCl, FeCl3.6H20, CuCl2) on the conversion of xylan to furfural was also investigated. The gamma-valerolactone-ferric chloride sent system exhibited the highest mean yield of furfural (56.50%). The next highest furfural yield of …

From Inner Segment To Outer Segment: Palmitoylation Of Photoreceptor Na+, K+-Atpase And The Importance Of Prcd In Photoreceptor Outer Segment Morphogenesis, Emily R. Sechrest

Graduate Theses, Dissertations, and Problem Reports (ETD)

Photoreceptors are specialized neuroepithelial cells which are optimized for efficient capture of light and initiation of visual transduction. These cells have several compartments which are very important for proper visual function and segregation of cellular processes, including the outer segment (OS), inner segment (IS), nucleus, and synapse. The IS houses all of the cellular organelles and biosynthetic molecular machinery the cell requires and is the site of protein synthesis. The light-sensing OS is a highly modified, primary cilium, which contains many stacks of double membranous discs which house proteins required for formation and maintenance of OS structure, as well as …

In Vitro And In Vivo Toxicological Evaluation Of Emissions From The Fused Filament Fabrication Three-Dimensional Printing, Mariana T. Farcas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fused filament fabrication (FFF), a three-dimensional (3-D) printing process, is an emerging technology that has recently gained wide popularity among both consumers and manufacturers. As filament is heated to above its glass transition temperature in a 3-D printer, a portion may undergo thermal decompostion, which releases ultrafine particles (UFP) and volatile organic compounds (VOCs) with potential adverse respiratory health effects are released into the air. This study's central hypotheses is that emissions generated during 3-D printing are toxic and exposure to these emissions induces pulmonary and systemic adverse health effects. Considering that currently, limited understanding is available on the health …

A Deeper Inquiry Into The Transitional Needs Of Student Veterans, Nathan Calvin Bridendolph Jr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Previous research revealed that veterans transitioning to post-secondary institutions have unique characteristics (PNPI, 2018; Wurster et al., 2013) and may suffer from a constellation of physical, psychological, and social problems that can impact their ability to perform to their full potential on campus (Ackerman et al., 2009; Alschuler & Yarab, 2016; Igielnik, 2019). Despite knowing the struggles student veterans (SVs) face when transitioning to campus, no studies were found that directly gain SVs’ input on what they believe will help their transition. The current study sought to gain a holistic perspective of SV needs by using the biopsychosocial model and …

An Examination Of The Start Now Dialectical Behavior Therapy-Based Intervention Plus A Behavioral Level System On Male Inmate Misbehavior, Aggressive Behavior, And Mental Health, Victoria Disciullo

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to evaluate the impact of the START NOW program + behavioral level system (BLS) in a self-contained therapeutic community (SCTC) on inmate misbehavior at a correctional facility in a southern state. The SCTC includes the START NOW manualized mental health treatment, recreation groups, process groups as needed, and additional positive reinforcement to target inmate prosocial behavior (i.e., level system to obtain privileges). Inmate data was evaluated at 3-months pre-START NOW intervention and 3-months post-START NOW intervention to determine if there were differences in the number of referrals to mental health services for misbehavior, write-ups …

The Role Of Tree Species, The Herb Layer, And Watershed Characteristics On Nitrogen Cycling In A Central Appalachian Hardwood Forest, Sian E. Eisenhut

Graduate Theses, Dissertations, and Problem Reports (ETD)

Little is known about the forest herbaceous layer’s contributions to nitrogen (N) cycling under varying levels of N deposition, at different stages of forest succession, in watersheds with different aspects, and under different tree species. The objectives of this study were to quantify nitrogen reductase activity (NRA) of two tree species, red maple and sugar maple, and surrounding common herb-layer species at the tissue (foliage, roots) and plot level and to assess how these species affect the concentrations of nitrate, ammonium, and organic N in soil water collected in lysimeters beneath these trees. Different watersheds located at the Fernow Experimental …

Various Operational Aspects Of The Extraction Of Critical Minerals From Acid Mine Drainage And Its Treatment By-Product, Zhongqing Xiao

Graduate Theses, Dissertations, and Problem Reports (ETD)

Acid mine drainage (AMD) is environmentally hazardous acidic water, which is continuously generated in large volumes at coal mining sites, typically when pyrite is exposed to oxygen and water. As its continuous migration under the geologic conditions, AMD tends to leach metal elements out from the surrounding minerals, leading to high concentrations of metal elements, like iron, aluminum, magnesium, manganese, lead, arsenic, as well as critical cobalt and rare earth elements (REEs). AMD must be treated and monitored to meet the effluent limits, under U.S.C §1251, the Clean Water act, before being discharged to receiving streams.

Recently, the research is …

Traditional And Modern Breeding Strategies Towards Developing Resilient Crops: Two Case Studies In Tomato, Estefania Tavares Flores

Graduate Theses, Dissertations, and Problem Reports (ETD)

Crop designing for resilience traits to produce satisfactorily under adverse conditions and scenarios is a priority for the future of global food security. In this thesis, two different approaches addressing major stress factors for tomato cropping are presented. We first aimed to domesticate tomato de novo as a high-salinity resistant crop using the wild relative, S. cheesmaniae via gene editing. We conducted single-gene and multiplex knock-out strategies using CRISPR/Cas9 genome editing to create loss-of-function alleles for the domestication-related genes BIF, CycB, J2, EJ2, MULTI, SP, SP5G, and FW11.3 in order to create a novel, halophyte tomato harboring an inherent high-salinity …

Utilizing The Coughing Rat Model Of Pertussis To Improve Vaccine Efficacy, Jesse M. Hall

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pertussis, also known as whooping cough, is a respiratory disease caused by the highly contagious, Gram-negative pathogen Bordetella pertussis (Bp). Infection occurs through inhalation of aerosolized droplets containing Bp, which then colonizes ciliated epithelial cells of the respiratory tract. Here, Bp expresses toxins and virulence factors that lead to leukocyte recruitment, paroxysmal cough, and impairment of host innate responses. Currently, in developed countries, acellular pertussis vaccines (aP; DTaP; Tdap) are used to prevent Bp infection and whooping cough disease. However, we currently realize that the aP vaccine efficacy quickly wanes resulting in a reemergence of pertussis. Recent …

Using Molecular Dynamics To Characterize The Relationship Between Membrane Components And Dynamics And Supramolecular Organization Of Membrane Proteins, Eric Sefah

Graduate Theses, Dissertations, and Problem Reports (ETD)

Molecular dynamics (MD) simulations have gained impetus as a technique for elucidating structural and dynamical information about membrane proteins (MP). In particular, coarse-grained (CG) MD simulations have provided valuable information about the relationship between membrane components and supramolecular organization of MPs. In this work MD simulations are used to characterize the effects of hopanoids on bacterial membrane dynamics and association in a model protein proteorhodopsin (PR), as well as the role of the extended C-terminus of the human adenosine receptor (A_2AR) on its dimerization. PR is found to dimerize in a manner that is dependent on both the …

Novel Mechanisms Of Neuronal And Hormonal Regulation Of Large Intestinal Electrolyte Transport, Andrew John Nickerson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The mammalian colon plays a vital role in maintaining overall electrolyte and fluid homeostasis in the body, and it possesses the ability to either secrete or absorb water depending upon the needs of the organism. Secretion or absorption of electrolyte ions, such as Na⁺, Cl^- and K⁺, drives the movement of water across the colonic epithelium – the inner gut lining where all transport takes place. Aberrant absorptive and/or secretory activity within the colonic epithelium is central to the pathogenesis of many gastrointestinal diseases, such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), constipation …

Targeting Calcium Homeostasis For The Treatment Of Multiple Myeloma, Osama M. Elzamzamy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Multiple myeloma (MM) is a hematological malignancy characterized by the neoplastic proliferation of the plasma cells. MM is a relatively rare cancer that accounts for about 1.8% of all cancers and is the second most common hematologic malignancies, and despite the advancement from untreatable to treatable malignancy, it is yet incurable. Calcium ions (Ca²⁺) play an important role as second messengers in regulating a plethora of physiological and pathological processes, hence cytoplasmic Ca²⁺ is tightly regulated with strict spatial and temporal control to initiate, maintain, and terminate appropriate signaling pathways and phenotypes including cellular proliferation, cell cycle …

Effects Of Nonexamples On Concept Formation, Catherine Louise Williams

Graduate Theses, Dissertations, and Problem Reports (ETD)

Concept formation is affected by the examples and nonexamples provided during training, but the degree to which examples and nonexamples should differ is unknown. Two experiments compared concept formation when different kinds of nonexamples were used during training. Both experiments included a within-subject comparison of concept formation following three training conditions: a) nonexamples that were more similar to the examples, b) nonexamples that are less similar to the examples, and c) no nonexamples. Arbitrary concepts were trained in Experiment 1 and concepts akin to those that could be taught in a classroom were trained in Experiment 2. Before and after …

Targeting The Cerebrovasculature In Sepsis: A Focus On The Brain Microvascular Endothelium, Divine C. Nwafor

Graduate Theses, Dissertations, and Problem Reports (ETD)

The blood-brain barrier (BBB) is a critical interface between the systemic circulation and the brain. It is a specialized multicellular unit composed of brain microvascular endothelial cells (BMECs), pericytes, a basement membrane, and astrocytic end foot processes. BMECs are a principal component of the BBB that provide the structural framework needed for the stringent transport of molecules into the brain. BMEC dysfunction permits the trafficking of neurotoxins from systemic circulation into the brain, which ultimately exacerbates BBB dysfunction and neuroinflammation. Studies have shown that BBB dysfunction is a key determinant of cognitive decline in sepsis. However, there are critical knowledge …

A Short Film That Addresses Substance Use Disorders In Rural Communities And Strategies In Prevention And Treatment Of Addiction, Sarah R. Poteracki

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research paper and accompanying video (link to the short film is here: https://drive.google.com/drive/folders/1etDnUmEvkOds8J0EKnq1gnFO79-GggjY) offer insight into the current state of research collaboration surrounding substance use disorders in West Virginia. This professional project explores the importance of collaboration both in a research and clinical setting and in the social realm. The film was shown at the 2nd annual meeting for the Appalachian Node of the National Institute on Drug Abuse Clinical Trials Network on February 25, 2021. I interviewed 14 researchers and experts from a diverse range of disciplines who shared insights and strategies in the prevention and treatment of …

Remediation Of Spatial Skills In First Semester Calculus Using Haptics-Based Applications, Kristen L. Murphy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Spatial reasoning is required for many topics in undergraduate mathematics as well as other STEM fields. In calculus specifically, there are few interventions that assess students’ spatial skills and provide remediation. Haptic technology is a novel approach to spatial skills remediation due to its unfamiliarity to students and its flexibility with regard to models. The purpose of this dissertation is two-fold: first, to determine what level of spatial abilities students possess upon entering an undergraduate calculus course, and second, to determine whether haptic feedback will enhance an intervention for improving spatial skills.

The dissertation research was a mixed-methods study using …

Improving Performance And Durability Of Intermediate Temperature Proton‐Conducting Solid Oxide Electrolysis Cells Via Materials Design And Catalyst Surface Engineering, Hanchen Tian

Graduate Theses, Dissertations, and Problem Reports (ETD)

Solid oxide steam electrolysis cell, a promising electrical-chemical conversion device for the next generation efficient hydrogen production and energy storage, has been actively studied because of their high energy conversion efficiencies and prospective applications as electrochemical reactors. After decades of research on protonic ceramic materials, remarkable advances have been made in the protonic ceramic electrochemical cells (PCECs) air electrode and electrolyte. However, the existing air electrodes are far from satisfying the requirements of practical applications, a series of issues, including the lack of active and durable electrodes, greatly limit the commercialization. To date, the systematic development of triple conducting catalysts …

Understanding Visitor Use At Florida Keys National Marine Sanctuary, Melissa Maria Hurtado Alvarez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Effective management and monitoring of visitor recreational use is fundamental in marine protected areas. It is common to hear that tourism and outdoor recreation are often qualified as double-edged activities, making valuable socioeconomic contributions but also causing degradation of the environment. The convergence of social and natural resource research and practices can help managers to create better policies that will maximize human benefits from, and minimize human pressures on, ocean and coastal environments. For this study online surveys were sent via Qualtrics to email addresses obtained from the state of Florida during the summer of 2020. The first paper assessed …

Quantitation Of Fentanyl And Metabolites From Blow Fly Tissue And Development Effects Of Fentanyl On Lucilia Sericata, Joseph Allen Cox

Graduate Theses, Dissertations, and Problem Reports (ETD)

Death investigations often involve collaborative work between different forensic disciplines, especially with regards to pathology, toxicology, and entomology. This dissertation investigates the effects of designer opioids on the development of blow fly (Lucilia sericata) larvae and the ability to detect and quantify the drugs and/or metabolites present in each stage of the fly’s life cycle. Therefore, we evaluated human tissue fortified with fentanyl at four treatment concentrations, and blow fly larvae were placed to feed on the drug infused tissue. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method was developed, validated, and applied to extract …

Environmental Controls On The Terrestrial Water Cycle In Forested Mountain Ecosystems., Luis Andrés Guillén

Graduate Theses, Dissertations, and Problem Reports (ETD)

Water is a key resource to natural ecosystems and human societies alike, and the water cycle is fundamentally linked to the climate and the characteristics of catchments. However, the challenges posed by environmental change makes it imperative to understand how the water cycle is affected by biotic and abiotic factors, in particular, in areas that are crucial sources of water like forested headwater catchments. Therefore, this doctoral dissertation aims to advance the knowledge on the dynamics between climate, vegetation and landscape that determine the water balance of forested mountain ecosystems. This document presents five chapters, an introductory chapter, three standalone …

Manipulating The Perineuronal Net In The Deep Cerebellar Nucleus, Deidre E. O'Dell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Perineuronal nets (PNN) are a type of specialized extracellular matrix in the central nervous system. The PNN forms during postnatal development but the ontogeny of the PNN has yet to be elucidated. Studying the PNN in the rat brain may allow us to further understand the PNN’s role in development, learning, and memory. The PNN is fully developed in the deep cerebellar nuclei (DCN) of rats by post-natal day 18. By using enzymatic digestion of the PNN with chondroitinase ABC (ChABC), we studied how digestion of the PNN affects cerebellar-dependent eyeblink conditioning (EBC) and performed electrophysiological recordings from DCN neurons. …

Bravery In The Delicate: An Actor's Exploration Of Thea Elvsted In Henrik Ibsen's Hedda Gabler, Mary Catherine Goff

Graduate Theses, Dissertations, and Problem Reports (ETD)

This purpose of this document is twofold: to examine the process of character creation over the course of my experiences performing the role of Thea Elvsted in West Virginia University’s spring 2020 production of Henrik Ibsen’s Hedda Gabler, and to define Thea’s dramatic significance to Hedda Gabler as a whole. The following text will provide a detailed look at the research I collected in the interest of building a cohesive view of the world of the play. Additionally, it will describe the various techniques I employed throughout the rehearsal and performance process of the production, with specific attention paid to …

Weed Recognition In Agriculture: A Mask R-Cnn Approach, Sruthi Keerthi Valicharla

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recent interdisciplinary collaboration on deep learning has led to a growing interest in its application in the agriculture domain. Weed control and management are some of the crucial tasks in agriculture to maintain high crop productivity. The inception phase of weed control and management is to successfully recognize the weed plants, followed by providing a suitable management plan. Due to the complexities in agriculture images, such as similar colour and texture, we need to incorporate a deep neural network that uses pixel-wise grouping for identifying the plant species. In this thesis, we analysed the performance of one of the most …

Effects Of Changes In Land Use Land Cover And Climate On Long-Term Total Dissolved Solids Trends In The Mid-Atlantic Region Of The United States, Melaine Edouard Kolimedje

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Mid-Atlantic region (MAR) of the U.S. is subjected to a variety of stressors that affect the headwaters of the major rivers. Some of these stressors are abandoned mine drainage, agriculture, municipal point sources, urban areas, out-of-basin diversions, competing water uses, rapid population growths in the lowlands, alterations in water availability due to climate change and habitat alteration. In addition to these regional stressors, the rapid population growths and energy sources shifting have resulted in changes in land use and land cover (LULC) over the last few decades. The interactive effects of LULC and interannual/long-term climate changes have resulted in …

Improving Characterization Of Small Molecules Using Enhanced Molecular Reaction And Ionization Techniques, Sandra N. Majuta

Graduate Theses, Dissertations, and Problem Reports (ETD)

Metabolomics is an emerging “omics” field, which is comprised of the study of small molecule metabolites in biological systems. It can be argued that the abundances of metabolites within biological samples, such as tissue and plasma, are indicative of various physiological states. Thus, metabolomics analysis has the potential in the clinical scientific field to be an effective tool for early disease diagnosis as well as for proposing and monitoring new treatments for diseases. However, full characterization of the metabolome complement is often hampered by the wide diversity of metabolites, which also exhibit an extremely large concentration range in biological samples. …

Pseudomonas Aeruginosa Vaccine Development Using Dual-Species Whole Cell And Subunit Vaccines, Catherine Bell Blackwood

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen which can cause severe, recurrent, and chronic infections. The pathogen is highly adaptable, and pneumonia caused by it is associated with high morbidity and mortality, especially in individuals with the genetic disorder cystic fibrosis. Antibiotic resistance amongst clinical isolates of P. aeruginosa is steadily increasing, and multi-drug resistant strains are prevalent. There is currently no vaccine available for commercial use against P. aeruginosa. For these reasons, we sought to understand the immunity to P. aeruginosa induced by whole cell vaccination and identify antigens for development of future subunit vaccines. In this dissertation, …