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Feasibility Of Applying Motion Magnification In Subsurface Defect Detection For Concrete And Fiber-Reinforced Polymer Specimens, Nagavardhani Malineni

Graduate Theses, Dissertations, and Problem Reports (ETD)

Irrespective of size and complexity, every civil infrastructure needs certain scrutiny regarding its structural health to ensure its serviceability during its lifetime. In olden times such scrutiny was done with the aid of destructive testing methods using sensors that required cumbersome and expensive installations and led to the destruction of at least part of the tested specimen. However, in recent times, many non-destructive methods, such as acoustic emission testing, electromagnetic testing, and laser testing methods have emerged, leaving the specified tested specimen undisturbed. With the advancement in sensor technology like motion magnification and with the help of access to high-speed …

Investigating The Role Of Nudt7-Mediated Peroxisomal Coa Degradation In The Regulation Of Hepatic Coa Levels And Lipid Metabolism, Schuyler Dan Adams Vickers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Coenzyme A (CoA) is a crucial cofactor required to support many essential metabolic processes, including fatty acid oxidation and synthesis, the TCA cycle, and bile acid synthesis. In order to support the proper functioning of these pathways, CoA levels within the cell must be properly regulated. The regulation of CoA levels is achieved through a balance between CoA synthesis and CoA degradation. While the regulation of CoA synthesis has been extensively characterized, the degradation of CoA has received less attention. As such, the contribution of CoA degradation to the regulation of metabolism is poorly understood. NUDT7 is a CoA-specific Nudix …

Nature Based Interventions For Human Health And Wellbeing, Samantha I. Moyers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background. Nature exposure is beneficial to human health and wellbeing, but the evidence base for nature-based interventions (NBI) is still developing. Heterogeneous naming conventions, study designs, and intervention contexts create difficulty in accessing and assessing the evidence for such programming. While NBI have been utilized in a wide array of human populations, no interventions have been reported among clinical health professions students. Aims. The objectives of this dissertation project were to review the existing body of NBI literature exploring health and wellbeing outcomes, and to conduct a feasibility trial of forest bathing, a type of NBI, among clinical students. Specifically, …

The Development Of A New Pertussis Booster Formulation Via The Implementation Of New Adjuvants And Utilization Of Alternate Routes Of Administration, Megan Ashley Dejong

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pertussis (whooping cough) is a respiratory disease caused by airborne transmission of the Gram-negative bacterium, Bordetella pertussis. Prior to the development of the first pertussis vaccines (whole cell (wP) vaccines), the incidence of pertussis was in the hundreds of thousands of cases per year, which led to the death of many children, as the infection is most severe in younger populations. Thankfully, the wP formulation resulted in a dramatic decrease in the number of annual pertussis cases, nearly eradicating the disease. However, as wP contained the whole B. pertussis bacterium (and its lipooligosaccharide (LOS)), reactogenicity issues became apparent, leading …

Cancer Promoting Neutrophil Extracellular Traps In The Pancreatic Ductal Adenocarcinoma Tumor Microenvironment, Abby Ivey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pancreatic adenocarcinoma (PDAC) is a very aggressive disease with an overall survival rate at 12%. This poor outcome at diagnosis is in part due to the lack of effective treatment options. The aggressive disease and unique tumor microenvironment generated during disease initiation and progression has contributed to the lack of effective therapeutic options. Chemotherapy options have not improved overall survival substantially, the effectiveness of radiation therapy remains controversial, and immunotherapies provide little to no benefit when added to current standards of care. Thus, there is a critical need for new therapeutics that can either target PDAC alone or be added …

Artificial Light At Night Disrupts Pain Behavior And Cerebrovascular Structure In Mice, Jacob Raymond Bumgarner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Artificial Light at Night Disrupts Pain Behavior and Cerebrovascular Structure in Mice

Jacob R. Bumgarner

Circadian rhythms are intrinsic biological processes that fluctuate in function with a period of approximately 24 hours. These rhythms are precisely synchronized to the 24- hour day of the Earth by external rhythmic signaling cues. Solar light-dark cycles are the most potent environmental signaling cue for terrestrial organisms to align internal rhythms with the external day. Proper alignment and synchrony of internal circadian rhythms with external environmental rhythms are essential for health and optimal biological function.

The modern human environment on Earth is no longer …

Fish Community Responses To Environmental And Anthropogenic Conditions In West Virginia, Katherine A. Adase

Graduate Theses, Dissertations, and Problem Reports (ETD)

While Appalachia is among the oldest region in the world, Central West Virginia watersheds have been impacted by anthropogenic stressors, including extractive mining and timber harvest industries, as well as urban development and agriculture, damns, fracking, mining, and land cover use. This study aims to understand how natural environmental and human altered gradients impact affect diversity of fish communities in streams and rivers throughout Central Appalachia. The first chapter aims to provide insights into the impact of the 2016 low head dam removals on taxonomic and functional fish communities in the West Fork River, West Virginia and the potential benefits …

Spatial Distribution And Insect Associations Of Frangula Alnus In The Allegheny National Forest, Pennsylvania: Implications For Management, Jennifer S. Greenleaf

Graduate Theses, Dissertations, and Problem Reports (ETD)

Glossy buckthorn (Frangula alnus) (Rosales: Rhamnaceae) is an invasive European shrub that threatens to displace native vegetation in roadsides and disturbed forests in North America due to its lack of natural enemies, long leaf-out periods, and its ability to survive in a multitude of soil conditions. While well understood in its native range, information regarding F. alnus in North America is lacking. Currently, the management of this invasive shrub relies on an understanding of where it can be found and thus where treatments should be applied. This research investigated the spatial distribution patterns of F. alnus and profiled …

Effects Of Climate Change And Landscape-Scale Forest Management On Avian Communities, Abundance, And Nest Success In The Appalachian Mountains, Hannah L. Clipp

Graduate Theses, Dissertations, and Problem Reports (ETD)

Birds are integral components of ecosystems and account for billions of dollars in tangible benefits to humans. As such, recent continental declines of bird species have ecological and economic consequences, providing the impetus for my dissertation research. I identified knowledge gaps and proposed novel questions about how birds in the Appalachian Mountains are influenced by changing environmental conditions due to climate change and forest management. The Appalachian Mountains encompass an important biogeographical region with high conservation value due to its myriad habitats and corresponding bird species diversity. Thus, there is a critical need to evaluate the effects of shifting climate …

Role Of Cytochrome P450-Epoxygenases And Adenosine A2a Receptor In The Modulation Of Vascular Response, Stephanie Onyeka Agba

Graduate Theses, Dissertations, and Problem Reports (ETD)

Coronary Artery Disease (CAD) is one of the leading causes of mortality worldwide, and endothelial dysfunction is a major precursor for cardiovascular diseases (CVDs). Arachidonic acids in the vascular endothelial membrane are metabolized by the cytochrome P450-epoxygenases (CYP-2Js and 2Cs) to produce epoxyeicosatrienoic acid (EETs). These anti-inflammatory metabolites or oxylipins have been shown to be cardio-protective against ischemic/reperfusion injury and mediate vasodilation through hyperpolarization of smooth muscle cells. However, the enzyme called soluble epoxide hydrolase (sEH), mainly hydrolyzes EETs to its inactive form (DHETs) and an increase in sEH upregulates Angiotensin-II type1-receptor and facilitates endothelial dysfunction.

we previously showed vascular …

Health-Related Quality Of Life And Disease Severity Among Patients With Idiopathic Pulmonary Fibrosis, Rowida Mohamed

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Predicting the survival of patients with Idiopathic pulmonary fibrosis (IPF) remains challenging due to the remarkable heterogeneity of the disease prognosis. Therefore, developing reliable prognostic tools is of utmost importance in IPF management. Nomograms currently represent the most accurate and discriminatory tools for predicting outcomes, with superior performance compared to the commonly used integer score approach. Therefore, developing a valid local nomogram to predict mortality among patients with IPF is warranted. Additionally, IPF is characterized by a high symptom burden significantly impacting Health-related quality of life (HRQoL). Accurately measuring HRQoL in IPF is crucial as it guides treatment decisions …

3d Printed Microfluidic Devices For Advanced Fluid Manipulation In Biomedical Applications, Kathrine Curtin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microfluidics are valuable devices in the biomedical field because of their remarkable ability to manipulate small volumes of fluid and facilitate reactions. Microfluidics have several significant advantages, including their portability, low cost, fast reaction times, and high surface area-to-volume ratios. Microfluidics be used for a multitude of applications, including bioanalysis, cellular assays, organ-on-a-chip, and separations. Microfluidics have great potential to overcome many challenges in conventional chemical and biological applications. However, complex fabrication procedures in silicon, glass, thermoplastics, and (polydimethylsiloxane) PDMS devices make rapidly optimizing and prototyping devices challenging. Microfluidic fabrication techniques have been a long-standing challenge in successfully translating these …

A Comparison Of Fish Health Indices Applied To Freshwater Species Of The Chesapeake Bay Watershed, Josiah B. Jensen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fish kills, increased disease prevalence, and endocrine disruption have been observed in multiple freshwater fish populations of the Chesapeake Bay watershed (CBW). Some of these health issues occur in conjunction with declining abundance. A combination of multiple stressors is believed to be weakening sensitive fish species in non-tidal regions of the CBW. Fish health indices such as Deformity, Erosion, Lesion, and Tumor counts (DELTs), and the Health Assessment Index (HAI) are simple tools designed to evaluate the general health of fish populations. Both indices could be widely applied in the watershed because they require limited training and equipment. However, their …

Conversion And Utilization Of Forest Logging Residues For Value Added Bioproducts In The Eastern United Sates, Wanhe Hu

Graduate Theses, Dissertations, and Problem Reports (ETD)

Four major studies were conducted in this dissertation to investigate the quality, availability, pretreatment, conversion, and utilization of forest logging residues in the eastern United States. The introduction and conclusion sections are found in the first and last chapters. The quality of various-year-naturally-decomposed softwood and hardwood forest logging residues from three subregions of the eastern United States was investigated in the second chapter. In the third chapter, the combustion and energy properties of hydrothermal, inert, and oxidative torrefied red maple were investigated. Finally, in the fourth and fifth chapters, KMnO₄ surface-modified logging residue hydrochars were used to investigate the …

Advancing Assessments Of Climate Change Vulnerability Of West Virginia Watersheds, Joseph T. Molina

Graduate Theses, Dissertations, and Problem Reports (ETD)

As climate change is becoming increasingly recognized as a threat to aquatic ecosystems, climate adaptation planning, in both the long- and short-term, is becoming more common in natural resource management. There is a need within conservation agencies to develop methodologies and assessments that support adaptation planning to efficiently disperse conservation dollars and effectively strengthen ecological and community resilience as climate changes continue. My thesis aims to provide West Virginia natural resources managers with climate vulnerability assessments that can be used to determine where and how conservation efforts should be administered. Additionally, I demonstrate a methodology that can be built upon …

The Eruption Of Disruption: The Manifestation Of Disrupting Whiteness In Secondary Social Studies In Appalachia, Elizabeth Disalvo Osborne

Graduate Theses, Dissertations, and Problem Reports (ETD)

This phenomenological dissertation explores the lived experiences of secondary social studies educators situated in the Appalachian region. Hermeneutic phenomenology was used as a philosophical and methodological approach to gather insights into this phenomenon. Interviews were conducted with three educators to capture their experiences from their childhoods, to their teaching careers, and into their current personal lives. These experiences were analyzed using a Whole-Part-Whole process to understand how they came to disrupt whiteness, the ways they did so, and their understanding of the impact disrupting whiteness for creating learning environments, developing curriculum and making instructional decisions. The findings revealed how these …

Diversification Of Ergot Alkaloid Biosynthesis In Natural And Engineered Fungi, Kyle Austin Davis

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ergot alkaloids are a complex family of tryptophan-derived mycotoxins produced by a diverse range of fungi that occupy a wide variety of ecological niches including soil saprotrophs, plant endophytes, pathogens of plants or insects, and opportunistic pathogens of humans and other mammals. Ergot alkaloids are a similarly diverse family of chemicals that elicit a variety of pharmacological activities in animals due to their resemblance to neurotransmitters and high binding affinity for neurological receptors, including those that bind adrenaline, dopamine, and 5-hydroxytryptamine receptors. These structural similarities allow us to create medicines aimed at treating a range of neurological diseases and disorders …

Investigating The Effects Of Flotation Restricted Environment Stimulation Therapy On Neural Networks In Chronic Pain Patients Via Functional Magnetic Resonance Imaging And Other Modalities, Tyler Austin Mcgaughey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chronic lower back pain (CLBP) is one of the leading causes of disability worldwide, with an estimated direct cost of over 600 billion dollars in the US alone. CLBP profoundly impacts the patient, their support team, and the healthcare system. Widespread changes to the patient's well-being, including mental health, are seen in CLBP and contribute to a downward spiral that often leads to disability. Chronic pain is poorly understood and is difficult to treat because of its subjective nature. Recent improvements in pain assessment in the clinic and in biomedical research have had a profoundly impacted the field. More than …

Forage Grass Growth Habit And Allelopathy In Birdsfoot Trefoil Germination And Establishment, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Birdsfoot trefoil (Lotus corniculatus L.) (BFT) is a high quality, tanniniferous forage legume that has been shown to mitigate gastrointestinal nematode (GIN) infection in sheep. Reliable establishment of birdsfoot trefoil in existing pastures is difficult due to BFT’s poor seedling vigor, susceptibility to crown and root rot, and, perhaps chiefly, competition from other pasture plants. Most existing pastures in the Northeastern United States are primarily composed of cool-season grasses, many of which can be highly competitive. A common form of interplant competition is allelopathy, which occurs when secondary metabolites produced by a plant inhibit a necessary growth process of …

Study Of Barrier Face Coverings And Respirator Exhalation Filtration Efficiency And Exhaled Air Velocity During Wear, Weihua Yang

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study aimed to assess the effectiveness of different types of respiratory protection, including National Institute for Occupational Safety and Health (NIOSH) Approved respirators and surgical masks in healthcare settings, as well as barrier face coverings (BFCs) used by the public as source control. The assessment was based on three components: total outward leakage (TOL), particle size distribution, and exhalation airflow velocity. To achieve this, the study utilized headform testing systems to measure TOL and airflow velocity. Specifically, a hard surface headform was used for NIOSH Approved respirators and surgical masks, while two soft surface headforms of different sizes were …

Sense And Sensitivity: Spatial Structure Of Conspecific Signals During Social Interaction, Keshav Ramachandra

Graduate Theses, Dissertations, and Problem Reports (ETD)

Organisms rely on sensory systems to gather information about their environment. Localizing the source of a signal is key in guiding the behavior of the animal successfully. Localization mechanisms must cope with the challenges of representing the spatial information of weak, noisy signals. In this dissertation, I investigate the spatial dynamics of natural stimuli and explore how the electrosensory system of weakly electric fish encodes these realistic spatial signals. To do so In Chapter 2, I develop a model that examines the strength of the signal as it reaches the sensory array and simulates the responses of the receptors. The …

Development And Characterization Of Monoclonal Antibodies Against Pseudomonas Aeruginosa, Jason Kang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Antimicrobial resistance (AMR) has emerged as a global crisis, posing significant challenges to the treatment and management of infectious diseases. The escalating prevalence of antibiotic-resistant bacteria has made many antimicrobial therapies ineffective, necessitating the development of alternative strategies to combat microbial infections. The rise of multidrug-resistant pathogens, such as Pseudomonas aeruginosa and Serratia marcescens, further exacerbates this problem, as these pathogens exhibit high levels of resistance to multiple antibiotics and cause severe infections in vulnerable populations. In response to the need for alternative therapeutic approaches, monoclonal antibodies (mAbs) have emerged as promising candidates for combating AMR. In this work, we …

Imitation Learning For Swarm Control Using Variational Inference, Hafeez Olafisayo Jimoh

Graduate Theses, Dissertations, and Problem Reports (ETD)

Swarms are groups of robots that can coordinate, cooperate, and communicate to achieve tasks that may be impossible for a single robot. These systems exhibit complex dynamical behavior, similar to those observed in physics, neuroscience, finance, biology, social and communication networks, etc. For instance, in Biology, schools of fish, swarm of bacteria, colony of termites exhibit flocking behavior to achieve simple and complex tasks. Modeling the dynamics of flocking in animals is challenging as we usually do not have full knowledge of the dynamics of the system and how individual agent interact. The environment of swarms is also very noisy …

Spatial Variability In Above Ground Carbon Within An Appalachian Forest, Megan A. Ponczek

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forests have long been recognized for their provision of air and water quality ecosystem services to society; but more recently, they have become valuable to the carbon credit markets used as a tool for mitigating climate change. Quantifying above ground carbon storage in forest ecosystems is essential for these carbon credit markets and can also provide insight into factors that control the spatial distribution of carbon in forests. The goal of this study is to assess the degree to which three factors: topography, tree species, and legacies of logging, impact the spatial variability in above ground carbon within a 400x500m …

Spatial Processing Of Conspecific Signals In Weakly Electric Fish: From Sensory Image To Neural Population Coding, Oak Everette Milam

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this dissertation, I examine how an animal’s nervous system encodes spatially realistic conspecific signals in their environment and how the encoding mechanisms support behavioral sensitivity. I begin by modeling changes in the electrosensory signals exchanged by weakly electric fish in a social context. During this behavior, I estimate how the spatial structure of conspecific stimuli influences sensory responses at the electroreceptive periphery. I then quantify how space is represented in the hindbrain, specifically in the primary sensory area called the electrosensory lateral line lobe. I show that behavioral sensitivity is influenced by the heterogeneous properties of the pyramidal cell …

The Birds And The Bees And The Willows: Understanding The Reproductive Biology Of Salix Nigra Marsh., Julianne Gmys Grady

Graduate Theses, Dissertations, and Problem Reports (ETD)

Salix nigra is a common tree-form willow found throughout the eastern United States and Canada in riparian and wetland habitats. Like other members of the Salicaceae, it is dioecious with separate male and female individuals. While Salix has been thought to be strictly insect pollinated and Populus wind pollinated, several species of Salix have had wind pollination present in varying amounts by species. S. nigra’s pollination syndrome has not been studied extensively, though it is assumed to be insect pollinated. Unlike other willows with wind pollination, S. nigra is basal on the Salix phylogenetic tree. This study was conducted …

Developing New Ionization Strategies For Characterizing Structural Flexibility, Heterogeneity, And Transformation Of Biopolymers, Daud Sharif

Graduate Theses, Dissertations, and Problem Reports (ETD)

Biopolymers, such as proteins and nucleic acids, serve important functions dictated by their structural folding. Information excavated from the intricate structural arrangements of biopolymers have been proved to be vital in diagnosing diseases, unveiling disease mechanisms, and developing new drugs. A continuous effort has been put to improve the characterization techniques of these biomolecules so that more precision can be achieved in terms of detailing their structure-function relationship. Mass spectrometry (MS) is one such technique that has become very relevant for structural biology with the emergence of soft ionization techniques such as electrospray ionization (ESI). A prerequisite to the application …

Intersectional Microaggressions And Disordered Eating In Asian American Women, Spring Szoka

Graduate Theses, Dissertations, and Problem Reports (ETD)

Disordered eating in college-age women is well-documented. Less is known about minoritized populations and disordered eating for college-age women, especially Asian American women. As Asian Americans are often subjected to the “model minority” myth, less research has been dedicated to understanding disordered eating for this population. Small, everyday slights motivated by the model minority myth and other racial stereotypes are known as microaggressions. Asian American women hold an intersectional identity that places them at risk for experiencing microaggressions from their identity as both a woman and as an Asian American. The purpose of this study was to examine relationships between …

Exploring Prenatal Care In A Rural Appalachian State: A Project Watch Study Of Barriers And Facilitators In All Births From May 2018 To March 2022, Madelin Gardner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: PNC is essential in protecting the health of birthing person and infant. Teenage and advanced maternal age (AMA) birthing persons are known risk factors for poor birth outcomes. However, less is known about whether these age groups are associated with inadequate PNC. Births to teenagers continue to be of concern in rural areas however, little is known about the association between inadequate PNC and poor infant outcomes in teenage populations. Previous studies have determined that greater risk of inadequate PNC has been linked to more rural areas compared to more urban areas. WV is the third most rural state …

Chemotherapy-Induced Pdl-1 Expression In Cancer-Associated Fibroblasts Promotes Chemoresistance In Nsclc, Gayathri Heenatigala Palliyage

Graduate Theses, Dissertations, and Problem Reports (ETD)

A cure for cancer is out of reach for most patients due to chemoresistance. Cancer-associated fibroblasts (CAFs), one of the most abundant types of stromal cells in the tumor microenvironment, are known to provide functional and structural support for tumor progression. These cells also play a role in cancer chemoresistance, but a detailed understanding of the process, particularly in chemoresistant lung cancer, is lacking. In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). We demonstrated that chemotherapy-stimulated CAFs …