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Abundance-Habitat Relationships And Aquatic-Terrestrial Habitat Selection Patterns For Wood Turtles (Glyptemys Insculpta) In The Upper Midwest, Jena Marie Staggs

Graduate Theses, Dissertations, and Problem Reports (ETD)

Due to range-wide population declines, wood turtles (Glyptemys insculpta) are under review for federal listing under the United States Endangered Species Act. Understanding how habitat associations and environmental conditions influence population dynamics and spatiotemporal activity patterns is fundamental for designing effective management strategies. Previous work on wood turtle abundance-habitat relationships focused on broad-scale habitat relationships in northeastern North America. However, regional differences in climate, habitat conditions, and land use patterns limit their applicability to much of the wood turtle’s Upper Midwest distribution. Much of our understanding of wood turtle activity patterns is based on radiotelemetry studies, which are biased towards …

Conditioned Reinforcement By Stimuli Correlated And Uncorrelated With A Schedule Of Food Reinforcement, Lillian L. Skiba-Thayer

Graduate Theses, Dissertations, and Problem Reports (ETD)

A conditioned reinforcer derives its function from a relation to an established reinforcer. Respondent views suggest that a stimulus acquires reinforcing properties because it is positively correlated with an established reinforcer. Alternatively, the information hypothesis suggests that a stimulus acquires reinforcing properties because it reduces uncertainty about the likelihood of an established reinforcer. Observing response procedures have been used to evaluate the function of stimuli positively correlated (S+), negatively correlated (S-), and uncorrelated (S1, S2) with established reinforcers such as food (with animals) or money (with humans). Verbal instructions have been shown to alter the extent to which correlated and …

Shear Bond Strength Comparison Of Bioceramics To Root Dentin., Yasmin Alma Hoffman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: The purpose of this study is to compare the push-out bond strength of four bioceramic materials to root dentin that are used during regenerative endodontics. The bioceramics being tested are: 1. Biodentine®(Septodont); 2. EndoSequence BC Putty Fast Set® (Brasseler); 3. NeoPutty® (Avalon Biomed); and 4. ProRoot MTA White® (Dentsply). A high bond strength of a bioceramic material to root dentin will provide excellent seal. An improved seal may correlate with less potential for contamination of the pulp space that is undergoing regenerative activity. This in turn will lead to long-term clinical success. The null hypothesis is that there is …

Navigating Place And Gender: A Multicontextual Critical Narrative Inquiry Of Rural Trans* Student Experiences, Jessie Lynn O'Quinn

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this critical narrative study was to understand how rural West Virginia trans* students navigate cultural norms of their rural home communities and higher education contexts. An essential part of this critical narrative was to provide rural trans* students with an avenue to share their unique experiences and give them a platform to share their voices. The resulting narratives suggested that the normative tensions rural trans* college students experience across contexts stemmed from negative regional experiences that reinforced traditional gender norms. Negative home contexts and experiences forced students to feel like they had to build walls and distance …

The Economics Of Pollution: Policy, Productivity, And The Structure Of Road Networks, Michael Cary

Graduate Theses, Dissertations, and Problem Reports (ETD)

Air pollution impacts economic activity, and economic activity creates air pollution. Understanding this endogenous nexus is critical as any policy designed to affect one will inevitably affect the other. My research explores both sides of this nexus. Specifically, I identify the economic effects from the implementation of an international climate agreement (the Kyoto Protocol); I determine the impact of air pollution on physical productivity and determine which pollutants are responsible for this effect; and I link urban form to vehicular emissions via the structure of road networks.

The first chapter of this dissertation studies the impact of the Kyoto Protocol …

Extension Of The Ergot Alkaloid Gene Cluster, Samantha Joy Fabian

Graduate Theses, Dissertations, and Problem Reports (ETD)

Specialized metabolites produced by fungi impact human health. A large portion of the pharmaceuticals currently on the market are derived from metabolites biosynthesized by microbes. Ergot alkaloids are a class of fungal metabolites that are important in the interactions of environmental fungi with insects and mammals and also are used in the production of pharmaceuticals. In animals, ergot alkaloids can act as partial agonists or antagonists at receptors for 5-hydroxytryptamine (serotonin), dopamine, and noradrenaline as ergot alkaloids have chemical structures similar to those neurotransmitters. Therefore, they affect insects and mammals that consume them and can be used to produce drugs …

Application Of Metagenomic And Molecular Microbiology Techniques To Elucidate Sources Of Fecal Pollution And Anthropogenic Antibiotic Resistance Genes To Surface Water: A Step Towards A “One Health” Approach To Watershed Management, Mehedi Hasan Tarek

Graduate Theses, Dissertations, and Problem Reports (ETD)

The use of fecal indicator bacteria, such as Escherichia coli, is a widely established regulatory and monitoring practice to detect surface water contamination associated with fecal pollution. However, the detection or quantification of fecal indicator bacteria alone does not accurately inform the sources of fecal pollution. The development of molecular and metagenomic methods that target the DNA of microorganisms has resulted in a host of new tools for monitoring fecal pollution and its sources, as well as for understanding emerging microbial threats, such as antimicrobial resistance. Antimicrobial resistance is a critical “One Health” challenge presenting a high risk to …

Beneficial Impacts Of Nod-Like Receptor X1(Nlrx1) Agonism In Lipopolysaccharide-Induced Inflammation, Mena Mansy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: The regulation of inflammation at the cellular and molecular level is a crucial aspect of homeostasis that is often overlooked, and most treatment options are limited to antibiotics and symptom management drugs. The development of novel molecular-targeted therapy options downstream of pathogen recognition receptors will fulfill the unmet need for therapy to effectively regulate excessive inflammation. NLRX1 is a unique NOD-like receptor that is anti-inflammatory in nature; it has been shown to negatively regulate the NF-kB pathway and has been involved in the pathology of a plethora of inflammatory disorders. This makes NLRX1 an emerging therapeutic molecular target for …

Social Comparison Theory: The Effects Athletic Influencers Have On An Appalachian Generation Z Men Audience, Aaron David Dickens

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many studies have highlighted how social comparison can influence self-efficacy for exercise, body dissatisfaction, and motivation to exercise, especially on social media; in addition, several studies have supported how attitude toward exercise and confidence toward exercise correlate with one another. However, these dependent variables have not been examined in the context of Generation Z men in Appalachia. Despite the lack of literature on this demographic, it is important to study Generation Z Appalachian men because of the frequent health problems this demographic faces, such as obesity. Therefore, this pretest-posttest between-subjects lab experiment investigated how an athletic influencer on social media …

Relationships Between Watershed Factors And Community Size Spectra Of The Fish And Macroinvertebrate Communities Of Mid-Appalachian Streams, Jarrett Hunter Landreth

Graduate Theses, Dissertations, and Problem Reports (ETD)

Freshwater lotic communities are complex, dynamic, and quick to respond to changes to their environment. However, some of the specific mechanisms driving those changes are yet to be fully understood. Here, I will use the community size spectrum (CSS) approach to assess how the CSS slope, elevation, and food chain length of the fish and macroinvertebrate assemblages as well as the combined CSS of 15 mid-sized (2nd-4th order) streams in north-central West Virginia respond across gradients of watershed factors (drainage area, mean drainage slope, mean drainage elevation, drainage % forest, and stream specific conductance). Field sampling was carried out in …

Leveraging Data Science To Promote Sustainable Resource Recovery From Organic Waste Streams In Rural Regions, Mohammed Tamim Zaki

Graduate Theses, Dissertations, and Problem Reports (ETD)

Conventional organic waste management practices such as landfilling contribute to global warming and degrade environmental and human health. Alternatively, organic waste can be treated to recover energy, nutrients, and carbon through resource recovery technologies. Data science could benefit such technologies by improving their efficiency through data-driven process modeling along with reducing environmental and economic burdens via life cycle assessment (LCA) and techno-economic analysis (TEA), respectively.

A critical review of 616 peer-reviewed articles published during 2002–2022 was conducted on the use of data science methods in resource recovery from organic waste. Although applications of machine learning (ML) methods have drastically increased …

Cluster Homolog Of Immunoglobulin-Like Receptor Genes In Chicken Immune Responses, Brandi A. Sparling

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores the identity and role of immunoglobulin-like (Ig-like) receptors in chickens, with focus on their implications in disease and disease progression. These receptors, wisely expressed across immune cells, interact with the major histocompatibility complex (MHC) class I molecules to modulate immune responses in mammals. Due to the insufficient representation of chicken Ig-like receptors in online databases, this study systematically annotates the chicken Cluster Homolog of Immunoglobulin-like Receptors (CHIR) genes using advanced bioinformatic techniques, aligning with the release of the 7th edition of the chicken genome assembly that comprises builds for a broiler and layer chicken. The analysis identifies …

Lipid Binding Properties Of Huntingtin As A Novel Therapeutic Target, Chathuranga Siriwardhana

Graduate Theses, Dissertations, and Problem Reports (ETD)

As protein aggregation is the defining hallmark of all amyloid diseases, a common therapeutic strategy is to develop molecules that inhibit aggregation. However, this approach has yielded limited success. Many amyloid proteins directly interact with lipid membranes. These interactions promote distinct aggregation pathways and often result in membrane damage leading to toxicity. As a result, directly targeting the ability of amyloids to bind lipid membranes represents a novel therapeutic strategy. As a proof of principle, the interaction between lipid membranes and mutant huntingtin protein (htt) aggregates was used to test this strategy. Mutant htt containing an expanded polygulatmine (polyQ) domain …

Exogenous Factors That Impact Huntingtin Aggregation, Adam Skeens

Graduate Theses, Dissertations, and Problem Reports (ETD)

While expansion of a polyglutamine (polyQ) domain is the immediate cause of huntingtin (htt) aggregation associated with Huntington’s Disease (HD), other cellular factors modify aggregation. These include interactions with cellular membranes, protein biding partners, molecular crowding, and proteinaceous seeds. Here, two important factors are biophysically characterized: 1) the interaction of htt with endomembranes and 2) proteinaceous seeds obtained from a variety of htt-derived peptides. In the first project, the aggregation of htt at bilayer interfaces and in the presence of divalent cations was investigated. A major cellular factor implicated in altered htt aggregation is the binding of lipids. Furthermore, the …

A Comparison Of Endodontic Root-End Filling Techniques: A Micro-Computed Tomography Analysis, Leslie P. Wolford

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction:

Researchers have compared the differences between hand compaction of root-end filling bioceramics with compaction of these materials by hand followed by ultrasonic activation (hand/ultrasonic compaction). Additionally, most of these researchers did not utilize Micro-Computed Tomography (Micro-CT) analysis. The purpose of this study is to evaluate total porosity volume of three different bioceramics when compacted by hand alone versus hand/ultrasonic compaction using Micro-CT Analysis.

Materials and methods:

Seventy-two human maxillary central incisors were selected for the study. All were subjected to orthograde endodontic treatment followed by root tip resection 3-mm from the apex at a 90° to the long axis …

The Role Of Nedd9 In Her2-Driven Breast Cancer., Marc Louis Purazo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tumor initiation is often driven by unrestricted proliferation. One such driver of proliferation is Human epidermal growth factor receptor 2 (HER2). HER2 is a receptor tyrosine kinase that is part of the epidermal growth factor receptor family (EFGR) that is commonly overexpressed in breast cancer. HER2 positive (+) breast cancers often respond to anti- HER2 therapy, yet many patients eventually develop resistance. Multiple mechanisms contribute to resistance, including activation of HSP90, PI3K/Akt or Src that rely on adaptor molecules (GRB2, p130cas, NEDD9). Neural precursor cell expressed, developmentally downregulated protein 9 (NEDD9) is an adaptor protein that promotes integrin signaling. We …

State Estimation, Covariance Estimation, And Economic Optimization Of Semi-Batch Bioprocesses, Ronald Hunter Alexander

Graduate Theses, Dissertations, and Problem Reports (ETD)

One of the most critical aspects of any chemical process engineer is the ability to gather, analyze, and trust incoming process data as it is often required in control and process monitoring applications. In real processes, online data can be unreliable due to factors such as poor tuning, calibration drift, or mechanical drift. Outside of these sources of noise, it may not be economically viable to directly measure all process states of interest (e.g., component concentrations). While process models can help validate incoming process data, models are often subject to plant-model mismatches, unmodeled disturbances, or lack enough detail to track …

Cell Signaling And Stress Response In The Yeast Saccharomyces Cerevisiae: A Study Of Snf1, Scott E. Arbet Ii

Graduate Theses, Dissertations, and Problem Reports (ETD)

Saccharomyces cerevisiae are yeast that are unicellular eukaryotic organisms that are well studied as a model organism for understanding fundamental cellular processes. The ability of yeast to sense nutrient availability is crucial for their survival, growth, and reproduction. Yeast cells use various mechanisms to sense and respond to nutrient availability, including transporter-mediated uptake, receptor-mediated signaling, and sensing of metabolites. The subcellular localization of nutrient-sensing components is crucial for yeast function in nutrient sensing and signaling. Protein complexes, such as the AMP-activated protein kinase (AMPK) pathway, in nutrient sensing and response, as well as the downstream effects of these pathways …

Specialized Metabolism In Retina, Retinal Pigmented Epithelium, And Testis, Siyan Zhu

Graduate Theses, Dissertations, and Problem Reports (ETD)

The retina and its neighboring retinal pigmented epithelium (RPE) are high energy-demanding and metabolically active tissues with specialized and complementary metabolism. They are metabolically interdependent and impact each other’s viability. Interestingly, many of the metabolic features in the retina and RPE are shared with the testis. For example, testis is also energy costly due to continuous sperm differentiation and it has similar metabolic inter-dependence between different testis cells. Both the retina and testis are vulnerable to mitochondrial metabolic impairments.

We conducted three research projects to understand 1) the nutrient utilization and communication in retina and RPE; 2) The profiling of …

Testing The Theory Of Resilience And Relational Load In The Context Of Empty Nesters, Lauren E. Fellers

Graduate Theses, Dissertations, and Problem Reports (ETD)

In examining the marital communication of recent empty nesters, the aim of this study was to test the theory of resilience and relational load (TRRL) (Afifi et al., 2016). The postparental period that follows directly after children leave their parents’ places of residence may present unique challenges for married individuals to navigate together. The TRRL offers a potential explanation as to why some married couples demonstrate resilience and flourish through a stressful season while others do not. To test the TRRL in this context, 113 married persons who became empty nesters within the previous 18 months filled out an online …

Associations Among Executive Functioning, Family Functioning, Adolescent Responsibility, And Adherence In Pediatric Inflammatory Bowel Disease, Cecily Newel Conour

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Inflammatory Bowel Disease (IBD) is a common cause of chronic pain for adolescents in the United States. Adherence to the treatment regimen is a significant concern, particularly for adolescents. Barriers to adherence are varied, but include cognitive factors, such as forgetting. Parent involvement is associated with increased adherence in this population, though adolescent involvement is less studied. Family functioning is associated with adherence to medication regimen across pediatric chronic illnesses, including IBD treatment regimen. To better inform clinical care, this study aims to understand the relations among adolescent’s responsibility, executive functioning, family functioning, and adherence. Methods: The current study …

Integrated Immunity-Based Methodology For Uav Monitoring And Control, Ryan G. Mclaughlin

Graduate Theses, Dissertations, and Problem Reports (ETD)

A general integrated and comprehensive health management framework based on the artificial immune system (AIS) paradigm is formulated and an automated system is developed and tested through simulation for the detection, identification, evaluation, and accommodation (DIEA) of abnormal conditions (ACs) on an unmanned aerial vehicle (UAV). The proposed methodology involves the establishment of a body of data to represent the function of the vehicle under nominal conditions, called the self, and differentiating this operation from that of the vehicle under an abnormal condition, referred to as the non-self. Data collected from simulations of the selected UAV autonomously flying a set …

Social Determinants Of Health And Functional Disability At Mid- And Late-Life, Carly Pullen

Graduate Theses, Dissertations, and Problem Reports (ETD)

The number and proportion of older adults living in the U.S. and globally are increasing. Not only are more adults living longer (Rania et al., 2021), there is evidence that people are

entering late life with more functional disability than in previous generations (He & Larsen,

2014). Social determinants of health include demographic and environmental influences than can impact a range of health outcomes beyond more direct risk factors (Duran and Perez-Stable, 2019). Using data from 243,693 community-dwelling adults who completed the 2020 Behavioral Risk Factor Surveillance System survey, we tested the associations among social determinants of health (i.e., age, …

Material Characterization Of Thermoplastic Polyurethane (Tpu) And Thermoplastic Elastomers (Tpe) For Development Of 3d-Printed Surrogate Organs For Medical Training, Anastasia Elizabeth Lucci

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cadaveric specimens are a necessary, albeit limited, resource for training medical students on basic surgical skills. The availability of surrogate 3D-printed organs would readily allow access to resources that could reduce or potentially eliminate the need for cadaveric specimens or, at a minimum, provide students the opportunity to practice with 3D-printed surrogates before transitioning to those specimens. This research focuses on determining which thermoplastic material most closely mimics mechanical properties such as hardness and stiffness of human organs and allows 3D printing surrogate organs to be used as safe, educational tools. Relatively “soft” materials such as thermoplastic polyurethanes (TPU) and …

Solid State Nmr Analysis Of Insect Wing Membranes, Samuel Eddy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Solid State NMR is a powerful tool for analyzing the chemical composition of insects. Previous studies have used Solid State NMR to investigate the chemical composition and investigate the sclerotization (tanning) process of insect exoskeletons. Acquiring sufficient exoskeleton sample for analysis has been relatively simple. This is however not the case for other components of an insect’s physiology, particularly the wing membrane material. The wings of an insect only compose a small percentage of their overall body mass, and many hundreds to thousands of insects must be processed in order to achieve a few milligrams of wing membrane sample. The …

Effects Of Light/Dark Cycle Shifts On Delay Discounting, Katya A. Nolder

Graduate Theses, Dissertations, and Problem Reports (ETD)

Shift-work conditions are a necessity for the constant provision of certain medical, industrial, and commercial services in modern society. Shift work is associated with the increased risk of many negative health outcomes, including type 2 diabetes, heart disease, reproductive issues, and cancer. Shift workers experience multiple levels of circadian-rhythm disruption, which may increase maladaptive decision making, and this may mediate the relation between shift work and negative health outcomes. Circadian-rhythm disruptions can be systematically investigated in the basic laboratory, and one type of procedure involves changing the light/dark cycle in the animal vivarium. The current study investigated effects of light/dark …

Rumen And Urine Amine/Phenol-Metabolome Of Beef Steers With Divergent Residual Feed Intake, Taylor S. Sidney

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cattle production is one of the most important agricultural industries in the United States. Despite its overall production efficiency, the industry is facing considerable adversities to its long-term sustainability due to population growth, decreased land availability, and the increase in global warming. Since feed efficiency imposes an immense influence on the cost of production for animals and is a major cost determinant for profitability, the improvement of feed utilization through the understanding of animal-to-animal variation in feed efficiency is pertinent to increase farm productivity and profitability. Residual feed intake (RFI) is a measure of feed efficiency and is defined as …

Biologically – Plausible Load Feedback From Dynamically Scaled Robotic Model Insect Legs, William Zyhowski

Graduate Theses, Dissertations, and Problem Reports (ETD)

Researchers have been studying the mechanisms underlying animal motor control for many years using computational models and biomimetic robots. Since testing some theories in animals can be challenging, this approach can enable unique contributions to the field. An example of a system that benefits from this modeling and robotics approach is the campaniform sensillum (CS), a kind of sensory organ used to detect the loads exerted on an insect's legs. The CS on the leg are found in groups on high-stress areas of the exoskeleton and have a major influence on the adaptation of walking behavior. The challenge for studying …

Missile Modeling And Simulation Of Nominal And Abnormal Scenarios Resulting From External Damage, James Manuel Floyd Iii

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis presents the development of a six-degree-of-freedom flight simulation environment for missiles and the application thereof to investigate the flight performance of missiles when exposed to external damage. The simulation environment was designed to provide a realistic representation of missile flight dynamics including aerodynamic effects, flight control systems, and self-guidance. The simulation environment was designed to be modular, expandable, and include realistic models of external damage to the missile body obtained by adversarial counteraction.

The primary objective of this research was to examine missile flight performance when subjected to unspecified external damage, including changes in trajectory, stability, and controllability, …

Long-Term Prescription Opioid Use, Cancer Stage, And Economic Burden Among Older Cancer Survivors With Non-Cancer Chronic Pain Conditions, Rudi Safarudin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Opioids are commonly prescribed to treat pain conditions, which are highly prevalent among older adults (≥65 years). Recent evidence shows that opioids may have carcinogenic effects through immunosuppression and free-radical pathways, which could promote cancer growth. Two population-based studies have reported an association of opioid use with incident cancer. Many individuals with non-cancer chronic pain conditions (NCPCs) may use opioids for a long term. However, the association of long-term prescription opioid (LPO) use with cancer stage at diagnosis is still unknown. Furthermore, evidence suggests that individuals with cancer and pre-existing NCPCs may persistently use opioids, even in the absence of …