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Graduate Theses, Dissertations, and Problem Reports (ETD)

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Subjective Norms, Attitudes, Body Mass Indices, And Self Esteem As Influences On Women's Body Image Behavior, Amy Martha Rogers Dec 2000

Subjective Norms, Attitudes, Body Mass Indices, And Self Esteem As Influences On Women's Body Image Behavior, Amy Martha Rogers

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to demonstrate associations between perception of body image among women and their body image behaviors. A study was needed to determine the extent to which subjective norms, attitudes, beliefs, Body Mass Index, and self esteem were associated with body image behaviors. The target population in this study was female students at West Virginia University. Data collection was facilitated through an anonymous mailed questionnaire sent to 400 female students at West Virginia University. Components of Theory of Reasoned Action were used to develop a questionnaire. Questions assessed participants' attitudes and beliefs, subjective norms, and outcomes …

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Evaluation Of Survival And Recovery Characteristics Of Bifidobacteria As Indicators Of Fecal Pollution Of Water, John Elmer Ravenscroft Jr. Dec 2000

Evaluation Of Survival And Recovery Characteristics Of Bifidobacteria As Indicators Of Fecal Pollution Of Water, John Elmer Ravenscroft Jr.

Graduate Theses, Dissertations, and Problem Reports (ETD)

The examination of aquatic environments for bacterial indicator organisms has proven to be a useful and well-established practice for the purpose of monitoring microbiological water quality. An ideal indicator of fecal pollution should be present in sufficient density to allow detection, present simultaneous with pathogen(s), incapable of aftergrowth in external aquatic environments, easy to enumerate, and exclusively of fecal origin. Unfortunately, none of the indicator organisms presently in use today meet all of these requirements. Bifidobacteria show promise as indicators of fecal pollution in water with the additional ability of potentially distinguishing between fecal pollution of human and animal origin. …

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Mycorrhizal Roles In Broomsedge Plants Under Phosphorus Limitation And Aluminum Toxicity, Jianchang Ning Dec 2000

Mycorrhizal Roles In Broomsedge Plants Under Phosphorus Limitation And Aluminum Toxicity, Jianchang Ning

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation examined roles of arbuscular mycorrhizal fungi, of which Glomus clarum is the primary species, in broomsedge (Andropogon virginicus) plants under limited Pi and elevated Al conditions in four studies. The inoculum originated from an acidic coal-mining site in Morgantown, WV. The inoculum of Glomus clarum from INVAM collection, which originated from an alkaline coal-mining site in WV, was also used in two of the studies. Plants were grown in an acid-washed sand culture system with a modified Hoagland's nutrient solution in a growth chamber.;Mycorrhizal fungal colonization pronouncedly enhanced growth of broomsedge plants under limited Pi and/or elevated Al …

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Characterization Of The Airway Epithelial Bioelectric Mechanisms Associated With The Effects Of Epithelium-Derived Relaxing Factor In Guinea Pig-Isolated Trachea, Richard Alan Johnston Dec 2000

Characterization Of The Airway Epithelial Bioelectric Mechanisms Associated With The Effects Of Epithelium-Derived Relaxing Factor In Guinea Pig-Isolated Trachea, Richard Alan Johnston

Graduate Theses, Dissertations, and Problem Reports (ETD)

In response to elevated serosal or mucosal tonicity, guinea-pig tracheal epithelium releases the non-nitric oxide, non-prostanoid, epithelium-derived relaxing factor (EpDRF), which subsequently diffuses to the underlying airway smooth muscle to initiate relaxation. Hypertonicity-induced smooth muscle relaxation via EpDRF involves epithelial Na+ and Cl- channels and is preceded by a depolarization of the transepithelial potential difference (Vms). By utilizing two different experimental systems, this study sought to characterize further the airway epithelial bioelectric mechanisms associated with the effects of EpDRF in guinea-pig isolated trachea.;First, this study attempted to elucidate which epithelial ion channels and/or transporters are involved in the depolarization of …

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Electrospray Ionization Mass Spectrometry For The Analysis Of Proteins And Small Molecules, Gregg A. Pratt Dec 2000

Electrospray Ionization Mass Spectrometry For The Analysis Of Proteins And Small Molecules, Gregg A. Pratt

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this document methods for the analysis of proteins and small molecules by Electrospray Ionization Mass Spectrometry (ESI-MS) are discussed. Section I contains an introduction to ESI-MS, including a brief history of mass spectrometry, a discussion of the current theories developed to explain the mechanisms at work during the electrospray process, and an explanation of Ion Trap Mass Spectrometry (IT-MS), the type of mass analyzer used for the studies outlined in subsequent chapters.;Section II is devoted to the analysis of proteins by ESI-MS. Chapter 2 gives an overview of protein structure and reviews previous work in the area of protein …

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Essential Oil Treatments To Control Varroa Destructor Anderson And Trueman 2000 (Formerly Varroa Jacobsoni Oudemans 1904) (Mesostigmata: Varroidae), Maria Mercedes Vargas-Sarmiento Dec 2000

Essential Oil Treatments To Control Varroa Destructor Anderson And Trueman 2000 (Formerly Varroa Jacobsoni Oudemans 1904) (Mesostigmata: Varroidae), Maria Mercedes Vargas-Sarmiento

Graduate Theses, Dissertations, and Problem Reports (ETD)

Varroa destructor Anderson and Trueman is the most serious pest of the honey bee Apis mellifera L. Varroa mites transmit several viruses, bacteria and fungi to the bees, which results in a condition called parasitic mite syndrome. There are no completely reliable treatments available to control the mite. Fifty-one beehives were artificially infested with varroa mites by using three different methods. Observations on mite population growth during three infestation attempts were made. All colonies were inspected for the presence of the small hive beetle, Aethina tumida Murray, no beetles were found. Thirty-five colonies were used to establish the efficacy of …

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Prognostic Factors For Treatment Outcome In Young Children With Autism, Susannah Grimm Poe Dec 2000

Prognostic Factors For Treatment Outcome In Young Children With Autism, Susannah Grimm Poe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the past decade, systematic applications of Applied Behavior Analysis (ABA) have proven an effective means of improving the prognosis for many young children with autism. But in every study indicating that a number of participants have experienced good outcomes from these interventions, there remain a sizeable number of participants who have not had the same success. This study was undertaken to examine the differences between the parental rankings for each of eight characteristics across three points in time for children with autism to determine who succeeds with this kind of treatment and who does not. These parental reports were …

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Carbon Products From Coal Liquefaction Fractions, Lizbeth Laureano-Perez Dec 2000

Carbon Products From Coal Liquefaction Fractions, Lizbeth Laureano-Perez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Processes for coal conversion are being evaluated for the development of carbon-product precursors. In the present work, coal hydrogenation is being considered as a procedure for tailoring the properties of the precursors for specific carbon products. The object of this project is to assess and evaluate such coal-derived materials for use as precursors for the production of carbon products. Coal liquefaction samples are made under various processing conditions, e.g. temperature, pressure, solvent. Softening points, ash content and coking values of the samples are determined at WVU by ASTM methods. These samples were also examined for coke microstructure and mesophase formation. …

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Predicting A Detailed Permeability Profile From Minipermeameter Measurements And Well Log Data, Shawn D. Nines Dec 2000

Predicting A Detailed Permeability Profile From Minipermeameter Measurements And Well Log Data, Shawn D. Nines

Graduate Theses, Dissertations, and Problem Reports (ETD)

Permeability, along with the porosity, comprises one of the two most important properties in petroleum engineering with respect producing hydrocarbon fluids.;It is standard practice in the petroleum industry to determine permeability in one of two ways. These are pressure transient testing and core analysis. Both methods are expensive in their own ways. This research focuses on a way to minimize or limit the need for both of these testing procedures.;The purpose of this research was to utilize well log data, mainly gamma ray and density, Minipermeameter values, and basic information such as depth and spatial coordinates to predict permeability in …

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Hazardous Materials Database, Veena Vadiala Dec 2000

Hazardous Materials Database, Veena Vadiala

Graduate Theses, Dissertations, and Problem Reports (ETD)

Handling and disposal of a retired object can be a major component of its Life Cycle Cost. Often, during dismantling of a retired object many hazardous materials are released. Disposal of hazardous materials also need to comply with various federal regulations. Agencies like Occupational Safety and Health Administration (OSHA) and the Environmental Protection Agency (EPA) set these safety regulations. It is possible to apply generic exposure and release controls to protect workers from hazardous materials. For the most cost effective hazard controls it is necessary first to identify the materials and their properties of primary concern. There is a need …

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West Virginians' Perceptions And Use Of Complementary Medicine, Joshua David Blevins Dec 2000

West Virginians' Perceptions And Use Of Complementary Medicine, Joshua David Blevins

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to determine how West Virginian's perceive and use complementary medicine. The first objective was to determine the frequency of respondents who had used at least one complementary therapy in the last year. The second objective was to determine the most commonly used complementary therapies among respondents. The third objective was to examine the demographic characteristics of respondents who used complementary therapies. The fourth objective was to determine if certain types of medical problems led to complementary medicine use among respondents. The fifth objective was to show the percentage of users who informed their physician …

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Factors Influencing Brook Trout (Salvelinus Fontinalis ) Abundance In Forested Headwater Streams With Emphasis On Fine Sediment, James Philip Hakala Dec 2000

Factors Influencing Brook Trout (Salvelinus Fontinalis ) Abundance In Forested Headwater Streams With Emphasis On Fine Sediment, James Philip Hakala

Graduate Theses, Dissertations, and Problem Reports (ETD)

The influence and relative importance of fine sediment on wild brook trout (Salvelinus fontinalis) population size was assessed. Brook trout production in headwater streams was inversely proportional to substrate permeability in one of two years, suggesting substrate composition influenced production. Results suggest the critical fine sediment size for brook trout in this study, is between 0.063 mm and 1.0 mm. Further, fine sediment (<0.063 mm) should not exceed 0.6--1.0% of spawning substrate, or negative population effects may be incurred. Under normal flow conditions, fine sediment was a principal determinant of juvenile trout abundance relative to other physical and biological factors. Adult trout abundance was principally a function of stream discharge, and is potentially further influenced by fine sediment impacts on juvenile recruitment. Spatially restricted food resources, created by drought-induced low flows in year 2 of the study, are believed to have over-powered mechanisms influencing trout abundance under normal flows.

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Diversity Of Ectomycorrhizal Fungi On Two Reclaimed Surface Mines Differing In Soil Properties, Betsy S. Kurnik Dec 2000

Diversity Of Ectomycorrhizal Fungi On Two Reclaimed Surface Mines Differing In Soil Properties, Betsy S. Kurnik

Graduate Theses, Dissertations, and Problem Reports (ETD)

I analyzed community structure of ectomycorrhizal fungi on white pine (Pinus strobus) on two reclaimed surface mines in eastern Ohio. One site was acidic and metal-stressed, the other was alkaline. Completely different community composition was found on the two sites by PCR/ITS/RFLP analyses of field collected mycorrhizae. However, Shannon-Wiener index values indicated that the two communities were equally diverse. A population of isolates of one species common to both sites was obtained from trap cultures planted with white pine. The identity of this species as Wilcoxina mikolae was established through DNA sequencing of the ITS region. The W. mikolae populations …

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Evaluation Of Maternal Serum Triple Screen As An Identifier Of Trisomy 21 Pregnancy, Jonnie Ann Lane Dec 2000

Evaluation Of Maternal Serum Triple Screen As An Identifier Of Trisomy 21 Pregnancy, Jonnie Ann Lane

Graduate Theses, Dissertations, and Problem Reports (ETD)

Maternal serum triple screen is used to identify women less than 35 years of age who are at an increased risk of having a fetus with Down syndrome. The screen identifies 5% of women tested as being at an increased risk but only 2--3% of these women actually have a fetus with chromosome abnormalities, indicating a high false positive rate. Medical records for 900 cases referred for maternal serum triple screen and maternal age were reviewed. The percentages of abnormal karyotypes in the two populations were not significantly different from each other or the expected value. While 3% of all …

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Exploring Visitor Meanings Of Place In The National Capital Parks - Central, Wei-Li Jasmine Chen Dec 2000

Exploring Visitor Meanings Of Place In The National Capital Parks - Central, Wei-Li Jasmine Chen

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study uses a new approach to interpretative research based on (1) understanding the meanings visitors attach to park resources, and (2) examining the connections that visitors made after attending an on-site interpretive program. The study was conducted at the National Capital Parks in Washington, DC. This study revealed that many visitors to the Lincoln Memorial, the Vietnam Veterans Memorial, and the Korean War Veterans Memorial (i.e. the Triangle) seek something of value for themselves, including everything from connecting with the past and rededicating themselves to the ideals of the nation. The study incorporated mixed method design, including purposeful sampling …

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Effects Of Pain And Fear Stimulus Intensity Levels On Pain Responding In Chronic Pain Patients, John Thomas Sorrell Dec 2000

Effects Of Pain And Fear Stimulus Intensity Levels On Pain Responding In Chronic Pain Patients, John Thomas Sorrell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fear has been found to increase and decrease pain responding in various contexts, and the effects of fear and pain stimulus intensity levels on pain responding have not been examined. Forty chronic pain patients were exposed to an algometer pressure pain device which stimulated different fingers; there were two levels of pressure pain (i.e., high and low). Also, there were two levels of induced fear (i.e., high and low). Significant main effects of pain stimulus intensity level were found on threshold pain time, tolerance pain time, and self-reports of fear and pain. Also, a significant main effect of gender was …

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Removal Of Uranium From Aqueous Wastes Using Electrically Charged Carbon Nanofibers, Shannon Lee Stover Dec 2000

Removal Of Uranium From Aqueous Wastes Using Electrically Charged Carbon Nanofibers, Shannon Lee Stover

Graduate Theses, Dissertations, and Problem Reports (ETD)

The presence of aqueous uranium wastes is a problem in the United States and their treatment/disposal is desirable. Treatment methods have been developed but result in concentration opposed to conversion to a disposable form. This technique involves recovery of uranium as a solid, providing an advantage over other methods. The technique utilizes carbon nanofibers as electrodes which successfully electrosorb uranium ions. Fibers with varying surface characteristics were evaluated in the removal process and all were determined to be equally and extremely effective. Various experimental parameters were evaluated including applied potential, pH, and flow rate. The critical applied potential at which …

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Breastfeeding Personal Efficacy Beliefs Of Women University Students, Ann Pollard Cleveland Dec 2000

Breastfeeding Personal Efficacy Beliefs Of Women University Students, Ann Pollard Cleveland

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many women do not initiate breastfeeding although breastfeeding promotes their baby's health. Three reasons why women do not breastfeed are women's mental organization, cultural beliefs about breastfeeding, and women's breastfeeding personal efficacy beliefs. The conceptual framework for personal efficacy beliefs is Bandura's social-cognitive theory (1992, 1995, 1997). This dissertation used a new research instrument to examine women university students' breastfeeding personal efficacy beliefs. The instrument was tested by mail survey techniques that resulted in a 70.6% respondent rate. University women's personal efficacy beliefs about breastfeeding factored into five factor subscales. In order of magnitude, the personal efficacy belief factors were …

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Development Of A Membrane Resistance Based Modeling Framework For Comparison Of Ultrafiltration Processes, David Albert Masciola Dec 2000

Development Of A Membrane Resistance Based Modeling Framework For Comparison Of Ultrafiltration Processes, David Albert Masciola

Graduate Theses, Dissertations, and Problem Reports (ETD)

A parametric study was conducted to develop a more general modeling framework as a basis for comparison of ultrafiltration membrane modules and separation processes. Discrete experiments were performed to examine the effects of operating parameters on permeate flux behavior in the tubular and high-shear rotary ultrafiltration (HSRUF) systems using a synthetic metal working (MW) fluid as a surrogate feed stream due to its relevance in industrial process and treatment applications. The conventional thin-film model was successfully applied to permeate flux data collected at low feed oil concentrations; however, model predictions at high concentrations were physically meaningless due to the formation …

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Effects Of Plant Flavonoids On The Fate Of Polynuclear Aromatic Hydrocarbons In Rhizosphere Soil, Xiujin Qui Aug 2000

Effects Of Plant Flavonoids On The Fate Of Polynuclear Aromatic Hydrocarbons In Rhizosphere Soil, Xiujin Qui

Graduate Theses, Dissertations, and Problem Reports (ETD)

High-molecular-weight polynuclear aromatic hydrocarbons (PAHs) are persistent in the environment although a wide variety of microorganisms can metabolize PAHs. In the past decades, laboratory and filed studies have shown that PAH degradation in soil is often limited by poor bioavailability and oxygen availability. Bound residue formation of PAHs with macromolecules of soil organic matter is an important fate mechanism. More recently, phytoremediation for PAH-contaminated soils is being explored. It is believed that PAH degradation may be enhanced in rhizosphere soil due to the improved aeration condition and the active soil microbial community sustained by root exudates. Whether certain root exudates …

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Induction Of Myosin Cross-Reactive Antibody And Cytolytic T Cell Responses In Mice With Streptococcus Pyogenes, Cynthia Ann Cunningham Aug 2000

Induction Of Myosin Cross-Reactive Antibody And Cytolytic T Cell Responses In Mice With Streptococcus Pyogenes, Cynthia Ann Cunningham

Graduate Theses, Dissertations, and Problem Reports (ETD)

Impetigo and pharyngitis are two common suppurative infections in man elicited by Streptococcus pyogenes that lead to autoimmune complications in susceptible individuals. Although the underlying mechanisms for the onset of post-streptococcal autoimmunity have yet to be established, experimental information gained thus far suggests that the resultant pathology is a consequence of immunological cross-reactivity between streptococcal antigens, namely the anti-phagocytic factor M protein and host proteins, specifically myosin. Experimental animal model systems developed to date have predominantly relied on the immunization of adult animals with M protein emulsed in adjuvant. In the laboratory we have undertaken studies with mice to examine …

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Quality And Postharvest Performance Of Cut Roses Grown In Root Media Containing Coal Bottom Ash, Marlene Karen Cross Aug 2000

Quality And Postharvest Performance Of Cut Roses Grown In Root Media Containing Coal Bottom Ash, Marlene Karen Cross

Graduate Theses, Dissertations, and Problem Reports (ETD)

The vase life of roses grown in coal bottom ash (CBA)-amended media was evaluated. CBA is enriched in calcium, a nutrient implicated in delaying senescence. Four rose cultivars, Cara Mia, Dakota, Santa Fe and Pink Osiana, were grown (from started eye plants) in four media: a 50% CBA medium and a peat: vermiculite medium amended with calcitic and dolomitic lime (1:1) were used as "high calcium" media, whereas a 25% CBA medium and a peat: vermiculite medium amended with dolomitic lime only were used as "low calcium" media. Yield was not different for roses from different media, and a subjective …

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Effects Of Acetaminophen On Estrogen-Responsive Alkaline Phosphatase In Ishikawa Endometrial Cancer Cells, Janet A. Dowdy Aug 2000

Effects Of Acetaminophen On Estrogen-Responsive Alkaline Phosphatase In Ishikawa Endometrial Cancer Cells, Janet A. Dowdy

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examines the effect of acetaminophen (APAP) on the estrogen-responsive gene alkaline phosphatase in Ishikawa endometrial cancer cells. Basal levels of alkaline phosphatase activity were significantly inhibited in cells treated with 0.03, 0.1, 0.3 and 1mM APAP compared to control. Alkaline phosphatase activity induced with 1nM estradiol was significantly inhibited in a concentration dependent manner by 0.0.1, 0.3 and 1mM APAP. APAP did not directly inhibit the activity of alkaline phosphatase at any of the concentrations tested. Increasing estradiol concentrations (0.01, 0.1 and 1nM) did not alter the level of inhibition of alkaline phosphatase activity by 0.1, 0.3 or …

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Assessing West Virginia Nipf Owner Characteristics And Preferred Assistance Topics, Daniel Joseph Magill Aug 2000

Assessing West Virginia Nipf Owner Characteristics And Preferred Assistance Topics, Daniel Joseph Magill

Graduate Theses, Dissertations, and Problem Reports (ETD)

Four hundred and fifty-one Non-Industrial-Private-Forest (NIPF) owners (49% response rate) in West Virginia responded to a survey of their forestry assistance needs. Most of the socioeconomic characteristics of the respondents, their reasons for owning the property, the types of activities they were engaged in on the property and the agencies that provided assistance by themselves significantly (Chi2 & ANOVA) differentiated those interested and not interested in obtaining assistance. However, when all 39 factors were considered together (Logistic Regression) only a small number of these factors were needed to explain the difference between landowners wanting and not wanting a specific assistance …

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Surface-Enhanced Raman Spectroscopic Studies Of The Orientation Of Organonitriles On Metal Colloids, Ramaa Nagapattinam Ramakrishnan Aug 2000

Surface-Enhanced Raman Spectroscopic Studies Of The Orientation Of Organonitriles On Metal Colloids, Ramaa Nagapattinam Ramakrishnan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The orientation of adsorbates on metal colloids was studied by Surface enhanced Raman spectroscopy. We studied the orientation of cyanopyridines, aminomethylpyridines and 4-biphenylcarbonitrile on gold and copper colloids as a function of concentration of the adsorbates. It was found that the orientation of the adsorbate did not change as a function of concentration when adsorbed on gold colloids and was end-on for all concentrations. The orientation of 4-biphenylcarbonitrile was flat-on on gold colloid. The orientation of the adsorbate changed with change in concentration when adsorbed on copper colloids. The orientation was flat-on for higher concentrations and end-on for lower concentrations …

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Scale, Ecological Fallacy, And The River Continuum Concept, Hope M. Childers Aug 2000

Scale, Ecological Fallacy, And The River Continuum Concept, Hope M. Childers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Concern over scale is not new, and it is not restricted to geographers. Spatial and temporal scaling is a conceptual and methodological problem for all sciences using geographic information. This paper teases out issues of scale and ecological fallacy from the literature and discusses how these issues influence the applicability of an influential theoretical framework in stream ecology, the River Continuum Concept (RCC). Investigators are faced with decisions regarding scale during sampling location selection, field data capture, and subsequent data interpretation. A thorough understanding of the heterogeneity of stream habitats and the life histories of the organisms being studied could …

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Dynamical Analysis Of Infant Social Referencing, Melissa Sue Atkins Aug 2000

Dynamical Analysis Of Infant Social Referencing, Melissa Sue Atkins

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of the present study was to examine relations between joint attention, language, maternal sensitivity, and temperament and the emergence of social referencing in infants and to closely observe variability in social referencing. Five infants, 7 months of age, were observed on a weekly basis for a period of four months. All five infants were engaging in social referencing behavior at the beginning of the study, which is much earlier than the literature indicates; therefore, the onset of the behavior was missed. However, results did indicate variability in social referencing behavior, in that infants referenced more in the laboratory …

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Effects Of Nitrogen And Water On Growth, Photosynthesis, And Leaf Properties Of Deciduous Tree Species With Consequences For Gypsy Moth Herbivory, Teri S. Myers Aug 2000

Effects Of Nitrogen And Water On Growth, Photosynthesis, And Leaf Properties Of Deciduous Tree Species With Consequences For Gypsy Moth Herbivory, Teri S. Myers

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study addressed the hypothesis that plants exposed to elevated N availability are more susceptible to drought and insect herbivory. I grew seedlings of gray birch (Betula populifolia), yellow birch (B. alleghaniensis), and red oak (Quercus rubra) for two years under varying levels of N and water availability in the field. Growth and biomass allocation were examined for the three species. Photosynthetic response was determined and feeding trials with gypsy moth larvae were conducted with both birch species. I found that the effects of nitrogen on growth and biomass allocation depended on species, while water had no significant effect on …

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Development And Application Electrochemical And Spectrophotometric Methods Based Upon Ac Potential Modulation For Characterization Of Hybrid Bilayer Membranes And Electroactive Self -Assembled Monolayers Supported On Gold Electrodes, Dmitri A. Brevnov Aug 2000

Development And Application Electrochemical And Spectrophotometric Methods Based Upon Ac Potential Modulation For Characterization Of Hybrid Bilayer Membranes And Electroactive Self -Assembled Monolayers Supported On Gold Electrodes, Dmitri A. Brevnov

Graduate Theses, Dissertations, and Problem Reports (ETD)

Applications of the electrochemical method, AC Voltammetry (ACV), and its modifications based upon monitoring ac current as a function of ac potential modulation are described. The first chapter involves investigating the effect of a short peptide, melittin, on both hybrid bilayer membrane (HBM) dielectric properties and HBM resistance to heterogeneous electron transfer. These effects are discussed in terms of three possible mechanisms by which melittin may affect HBMs. In the second chapter, a modification of ACV, second harmonic ACV, is considered. A data processing method is suggested to analyze data collected at high perturbation frequencies at which the effect of …

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Structural Determinants Of Murine Leukemia Virus (Mlv) Reverse Transcriptase (Rt) Important For Fidelity And Drug Resistance In Vivo, Elias Konstantine Halvas Aug 2000

Structural Determinants Of Murine Leukemia Virus (Mlv) Reverse Transcriptase (Rt) Important For Fidelity And Drug Resistance In Vivo, Elias Konstantine Halvas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Error-prone DNA synthesis by retroviral reverse transcriptases (RTs) is a major contributor to variation in retroviral populations generating mutants that exhibit altered host tropisms, resistance to antiviral drugs, and the ability to escape the host's immune system. To identify structural elements of murine leukemia virus (MLV) RT important for fidelity and drug-resistance in vivo, we developed a D17-based encapsidating cell line (ANGIE P) which is designed to express the amphotropic MLV envelope. ANGIE P cell line also contains an MLV-based retroviral vector (GA-1), which encodes a wild-type bacterial beta-galactosidase gene (lacZ) and a neomycin phosphotransferase gene. Transfection of ANGIE P …

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