Digital Commons Network^™

Adverse Childhood Experiences Predict Mortality Risk: The Role Of Social Support & Social Strain, Meredith A. Willard

Graduate Theses, Dissertations, and Problem Reports (ETD)

Childhood adversity has long-lasting negative effects across the lifespan including increased mortality risk. The love and support individuals receive from others, also known as social support, has shown to be a protective factor against ACEs. However, little research has investigated the amplifying effects of social conflict and strain that often accompanies social relationships. Utilizing data from the Midlife Development in the U.S. (MIDUS) study, I tested whether higher levels of social support would buffer the negative effects of adverse childhood experiences on mortality risk, and whether higher levels of social strain would amplify these associations. The sample included 6,150 participants …

Three Essays On Misinformation, Mistrust And Their Influence On Public Health Policy, Eli Kochersperger

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the first chapter I identify the impact of fentanyl exposure misinformation- namely, the erroneous belief that momentary, passive contact with the potent opioid fentanyl can be seriously harmful- on first responder behavior during overdose events, and on overall opioid-related mortality. I examine changes in opioid-related mortality following one particularly well-covered episode involving an Ohio police officer in 2017, wherein the officer appeared to experience an acute opioid overdose after touching what was believed to be fentanyl. Employing a synthetic differences-in-differences identification strategy, I find areas with greater media exposure to this misinformation exhibit marked increases in opioid overdose deaths; …

Analysis Of Mutations In Protein Kinase Ck2 Linked To Okur-Chung’S Neuropathy, Shivani Maan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The studies described here focus on mutations in protein kinase CK2 linked to ‘Okur-Chung’s Neuropathy’ (OCN), a neurodevelopmental disorder that involves 10 distinct mutations in the catalytic subunit CK2α. It has, however, remained unresolved if these mutations impair CK2α activity or if they also impair association with the regulatory CK2β subunit, which is necessary for assembly of the α2β2 holoenzyme. I describe efforts to resolve these questions for two mutations, Y50S and D175G. The former is thought to reside at the CK2α-CK2β interface, whereas the latter is located proximal to the active site. I therefore hypothesized that Y50S should perturb …

Transparent And Conductive Gallium Oxide Electrode For Simultaneous Recording And Optogenetic Stimulation, Christopher Patrick Carey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neural electrode technology has been around for centuries since the times of Galvani. In early electrophysiology experiments metal wires were used to induce contractions in dissected animals. The metal wire electrode has since been a standard tool to both stimulate and record neural activity. In the past two decades, a new strategy for neural stimulation has been formulated based on the emergent field of optogenetics. Optogenetics refers to the use of light-sensitive proteins genetically imbedded in the membrane of a neuron to elicit neural activity. This technique offers more selectivity in the stimulation of neurons. Typical optogenetic neural electrodes, or …

Mechanistic Insight Into Maternal Electronic Cigarette Exposure And Its Effect On Offspring Cerebrovascular And Neurocognitive Health, Amber Victoria Mills

Graduate Theses, Dissertations, and Problem Reports (ETD)

Electronic cigarettes (Ecigs) have replaced smoking within the last decade as the preferred nicotine delivery system because of their advertising to the younger populations. Marketing has sold the idea that use of Ecigs are safer than smoking and this messaging has resonated with youth and other vulnerable populations such as pregnant women. Despite over 2.8 million children under 18 (Annual National Youth Tobacco Survey) deciding to vape, there is little information about what this will do to the next generation. Further, it is that same population that will soon reach an age when they will want to have children. There …

Effect Of Haemonchus Contortus Excretory/Secretory Protein On Differences In Host Neutrophil Migration, Hannah Grace Teddleton

Graduate Theses, Dissertations, and Problem Reports (ETD)

An excretory/secretory (E/S) product, common to many helminths, is neutrophil inhibitory factor (NIF) which negatively affects neutrophil migration and activity. Due to differences in neutrophil accumulation between parasite resistant St. Croix STC and parasite susceptible Suffolk (SUF) sheep, we hypothesized that Hc-NIF may inhibit migration of SUF and not STC neutrophils. Previous studies have demonstrated that Hc produces NIF but is not commercially available. To test our hypothesis, neutrophils were cultured from STC and SUF sheep in the presence of Ancylostoma caninum-derived NIF (Ac-NIF) and measure chemotaxis to Interleukin-8 (IL-8), HcLA or H. contortus third-stage excretory/secretory (E/S) products. Neutrophils were …

Machine Learning And Rna Bioinformatics, Jason Rafe Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

The applied science of bioinformatics encompasses computational analysis of molecular biology data. Advances in genomics and DNA sequencing technology have enabled computational analysis of ribonucleic acids (RNAs), which play diverse and critical roles in most cells. To assist the study of human RNA, we trained machine learning models on RNA nucleotide sequences, devoid of domain knowledge. We built models that distinguish long non-coding lncRNA from protein-coding mRNA, and models that predict the cytoplasmic vs. nuclear preferences of lncRNAs. In a review of published lncRNA subcellular localization classifiers, we show that the commonly used validation protocol generates optimistic performance measures, and …

Selective Recovery Of Various Critical Metals From Acid Mine Drainage Sludge, Gorkem Gecimli

Graduate Theses, Dissertations, and Problem Reports (ETD)

Non-fuel minerals and commodities vital to the modern economy and national security are known as critical minerals. They are essential to modern high-tech industries, defense applications, clean technology, fuel cells, mobile phones, high-capacity batteries, permanent magnets, phosphors, and metal catalysts, among other things. Over fifty elements are deemed essential to varying degrees (European Commission, 2017; Department of Energy, 2020; Burton, 2022). Given their current position, a single supplier or small group of producers can easily dominate the mineral pricing as they supply the majority of these essential minerals. Due to the extended area of usage, ongoing population expansion, and increased …

Comparison Between Coronally Advanced Flap With Sub-Epithelial Connective Tissue Graft And Tunnel Technique With Sub-Epithelial Connective Tissue Graft In Achieving Mean Root Coverage At Multiple Recession Defects: A Retrospective Study, Christian Daniel Davila

Graduate Theses, Dissertations, and Problem Reports (ETD)

Aim: The primary objective is to retrospectively compare the mean root coverage (MRC) achieved using a coronally advanced flap and sub-epithelial connective tissue graft (CAF + CTG) with that achieved using the tunnelling technique and sub-epithelial connective tissue graft (TUN + CTG) for the treatment of multiple recession defects. The secondary objectives are to compare the proportion of complete root coverage (CRC) achieved with CAF + CTG compared to TUN + CTG, compare the proportion of CRC achieved in these two techniques with respect to level of training (American board-certified periodontist or Graduate periodontics residents) and to evaluate the …

Effects Of Land Cover Changes And Hydropower Development On Fish Communities In Amazonian Floodplain Rivers, Samuel Baron Grinstead Hessburg

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Amazonian River systems, diverse fish communities depend on the floodplains for providing feeding opportunities and critical spawning habitat during the seasonal flood pulse, which annually submerges floodplain forests. In the Amazon, large-scale deforestation and hydroelectric dam construction can have profound impacts on entire ecosystems by altering river-floodplain connectivity. This study attempts to improve understanding of Amazonian fish responses to these two stressors. The first chapter aims to understand how deforestation impacts floating meadow fish assemblages. For this study, we surveyed fish in floodplain lakes along the lower Amazon River, recording their abundance in relation to forest cover and local …

Puberty-Associated Changes In Kiss1r, Mc3r, And Mc4r In The Ewe, Ashleigh Caitlyn Thomson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The neural mechanisms that control the onset of puberty are not completely understood. In livestock, the onset of puberty relies on an increase in gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH). Kisspeptin, neurokinin B, and dynorphin neurons (KNDy) and their receptors, Kiss1r, NK3R, and KOR, respectively, are important regulators of puberty. Previous data from our laboratory has shown that KNDy neurons may be responding to other inputs, including the melanocortin system, that will affect the timing of puberty. Given this, we examined kisspeptin receptor (Kiss1r) mRNA expression in GnRH neurons as well as examining melanocortin 3 and 4 receptor …

S(+)-Propylnorapomorphine Hydrochloride Alters Mutant Huntingtin Aggregation And Membrane Interactions, Gabriella M. Pettipas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder resulting from an abnormally long CAG repeat in the huntingtin gene. This results in an expanded polyglutamine (polyQ) domain in the huntingtin protein (htt) that directly leads to self-assembly of huntingtin (htt) into a variety of aggregate species that underlie cellular toxicity. Interaction of protein aggregates with a variety of lipid membranes has been linked to disease pathology. Here, the ability of a small molecule S(+)-propylnorapomorphine (NM) hydrochloride to modulate htt-exon1(46Q) aggregation and interactions with lipid membranes was determined. ThT assays were performed to quantify fibril formation of htt in the …

Estimating Home Range Size And Density Of White-Tailed Deer (Odocoileus Virginianus) In West Virginia, Sarah M. Pesi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Big game hunting is an important source of revenue and recreation in the United States. Of big game species, white-tailed deer (Odocileus virginianus) are the most heavily pursued. White-tailed deer are a widespread generalist species, capable of existing at high densities. Hunting is often used as the primary method of managing deer populations, particularly in the eastern United States, where there is a lack of large predators. When populations reach high densities, there can be negative effects on the environment and conflicts with humans. Managers must consider both creating adequate hunting opportunities and reducing negative impacts when managing …

Life After Sport: A Program Development Plan For Student-Athletes, Loryn Shawn Frame

Graduate Theses, Dissertations, and Problem Reports (ETD)

College athletics stakeholders have had growing concerns regarding student-athletes’ transition out of collegiate athletics. Research specific to student-athletes following college athletics has explored mental health, role identity, and occupational participation. This program development plan addresses occupational therapy’s role in assisting student-athletes transition out of collegiate athletics. The needs assessment conducted with West Virginia University athletic staff revealed two gaps in programming: athletic identity including leisure exploration, and a program to help student-athletes who want to enter health professional programs. Life After Sport comprises two programs, one to help student-athletes transition into a health professional program, titled Athletes in Medicine (AIM), …

Women In Extension Persevering In Leadership Roles, Lexis Danielle Trickett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the last several decades, the rates at which women have been pursuing higher levels of education have steadily increased. However, there is a discrepancy between the number of women graduating, and the amount entering the workforce, specifically in leadership roles. The purpose of this study was to share a current profile of women in the West Virginia Cooperative Extension Service by allowing them to describe their career journeys, supports and barriers they may or may not have faced, and mentoring experiences. By using a non-experimental quantitative methodology, a population of male and female Extension Agents and Specialists were surveyed …

The Names Of All Wild Things: Field Guides And Environmental Thinking, Frank Izaguirre Phd

Graduate Theses, Dissertations, and Problem Reports (ETD)

The project first traces the earliest origins of natural history writing, surveying how natural history writing from antiquity presented and categorized the living world, and then how the rise of print culture and growing popularity of natural history writing would facilitate the rise of European empires. Values and conventions emerged that would later feed into the field guide, like the pairing of text and illustration to aid identification and the decontextualizing of plants and animals from their environments.

Near the end of the nineteenth century, the field guide genre emerged. Some wildly popular books, mostly written by women, emphasized the …

Characterization Of Host Cell Specific Interactions During Spotted Fever Group Rickettsiae Infection, Emerson Towey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Vector-borne diseases have been increasing over the past few years due to changes in climate and disturbances in the vectors’ natural environment. To mitigate the risks of these diseases, it is necessary to understand pathogen biology and how it interacts with a host to cause disease. Here we focus our attention on the tick-borne pathogen, Rickettsia rickettsii. R. rickettsii is a member of the spotted fever group (SFG) Rickettsiae and is the etiological agent of Rocky Mountain spotted fever (RMSF), a fatal human disease that is lacking an effective vaccine. Members of the bacterial genus Rickettsia are obligate intracellular endoparasites …

A Mixed Methods Approach To Evaluation And Modeling Of Ergonomic Stressors Due To Mass Decedent Handling, Vaishakhi Suresh

Graduate Theses, Dissertations, and Problem Reports (ETD)

Work-related injuries, illnesses, and musculoskeletal disorders (MSDs) significantly impact the U.S. healthcare industry, leading to lost working days, high medical costs, and increased turnover rates among healthcare workers (HCWs). Increased physical and mental health risks due to the handling of deceased during COVID-19 pandemic has exacerbated these issues. While the ergonomic risks of patient handling are well-studied, limited research exists on the ergonomic risks of manual handling of the deceased. Therefore, the primary objective of this study was to explore the challenges associated with decedent handling so that effective safety interventions to reduce the risk of injury and mental distress …

An Observational Census Of Post-Merger Galaxies And Supermassive Black Hole Pair Evolution, Gregory Walsh

Graduate Theses, Dissertations, and Problem Reports (ETD)

Massive galaxy mergers are a fundamental consequence of the dynamic evolution of the Universe and play a central role in the evolution of galaxies. Because all massive galaxies harbor a central supermassive black hole (SMBH; M ≥ 10⁶ M_⊙), studying these merging systems with electromagnetic (EM) techniques constrains galaxy evolution models and provides a systematic means to examine the astrophysical mechanisms that facilitate the growth of SMBHs. These growing SMBHs are observable across the EM spectrum as Active Galactic Nuclei (AGN). Massive galaxy mergers are a natural formation mechanism for an SMBH pair, eventually evolving into an …

On Confidence And Sense Of Belonging In Cybersecurity Students: Analysis & Prediction, Sadaf Amna Sarwari

Graduate Theses, Dissertations, and Problem Reports (ETD)

In recent years, there has been a rapid expansion of cybersecurity programs across higher education institutions in response to the widening skills gap in the cybersecurity job market. This study adopts quantitative and qualitative approaches to identify factors influencing West Virginia University (WVU)’s LANE Department of Computer Science and Electrical Engineering (LCSEE) students’ confidence and sense of belonging in the cybersecurity field. The results are based on data collected from surveys administered to LCSEE students in April 2022 and April 2023. The responses were analyzed using descriptive & inferential statistics and logistic regression techniques. Additionally, the 2023 data was utilized …

Light Wavelength Modulates Search Behavior Performance In Zebrafish, Matthew Reid Waalkes

Graduate Theses, Dissertations, and Problem Reports (ETD)

Long-term internal states drive many behaviors in animals. Zebrafish is a common vertebrate model conducive to finding the neural coding to these behaviors. In the Horstick lab, the larval zebrafish light search behavior is central to our research. This behavior, which is the locomotor response to the loss of light in larval zebrafish, is controlled by multiple neural populations that drive different internal states. The sensory neurons initially responsible for this behavior are photosensitive due to the expression of light-absorbing proteins called opsins. Opsins vary in the ranges of wavelengths of light that they absorb, and are expressed in distinct …

Bioenergy Cropping Systems Established On Marginal Lands: Effects Of Land Use, Energy Crop, And Fertility Amendments On Soil Health, Mica M. Keck

Graduate Theses, Dissertations, and Problem Reports (ETD)

Bioenergy crops offer a promising strategy for renewable energy production, accompanied by carbon (C) storage benefits when managed sustainably. Utilizing marginal lands for energy feedstock production presents an opportunity for bioenergy generation and concurrent climate mitigation without competing with food crops. However, achieving optimal yields requires strategies to restore soil fertility, which require increased understanding of the interactive effects of prior land use, energy crop species, and fertility amendments available. This study, conducted as part of the Mid-Atlantic Sustainable Biomass (MASBio) Consortium, investigated the effects of biochar incorporation—a C-rich charcoal derived from biomass, intended for soil enhancement—on bioenergy cropping systems …

Estradiol’S Role In Timely Puberty Onset In The Ewe, Eliana Gerardine Aerts

Graduate Theses, Dissertations, and Problem Reports (ETD)

In female sheep, the release of gonadotropin-releasing hormone (GnRH) from the hypothalamus triggers luteinizing hormone (LH) to be released from the pituitary. Luteinizing hormone, in turn, acts on the ovaries to elicit estradiol (E₂) release. E₂ acts in a negative feedback manner to control the amount of LH released. The increase in LH release that drives puberty onset results from a decrease in sensitivity to E₂ as the animal ages. However, the neural mechanisms mediating this change in sensitivity are not known, as is where in the hypothalamus E₂-negative feedback occurs. In the arcuate …

The Impact Of Occupational Therapy In Lactation Services, Sarah Adrienne Mcaninch

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many benefits to breastfeeding exist for both mothers and infants, yet exclusive breastfeeding rates remain low across the nation. These benefits include decreased chance of infections for infants, low infant mortality, and decreased chances of breast and ovarian cancers for mothers (Binns et al., 2016; CDC, 2023). Education strategies have recently become imperative for health organizations to help mothers sustain their ability to breastfeed within the recommended six-month timeframe. Current literature on education supports that women who receive education at some point in their lactation journey are more likely to reach the six-month period and have more confidence in their …

Finding “A Good Thing”: Embodying The Traumatic Sustainably, Erika Kuhn

Graduate Theses, Dissertations, and Problem Reports (ETD)

Contemporary dramatic work such as the play Anatomy of a Suicide by Alice Birch demands a significant toll on the actors embodying the characters. Western pedagogies that emphasize a need for the utmost authenticity must be merged with the modern practice of self-care and trauma informed boundaries. This document outlines the merging of traditional acting techniques of characterization with solutions for self preservation such as preparation, mental and physical upkeep, a personalized set of character de-rolement practices, and social rituals. Through an analysis of the text itself, its context, author, and the lens of one actor’s experience portraying the character …

Examining Invalid Brief Child Abuse Potential Inventory Profiles Within A Randomized Controlled Trial For Toddler-Aged Children And Their Parents, Lindsay Rebecca Druskin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Child abuse is a significant public health concern that impacts children worldwide. Efforts to connect at-risk parents with prevention and intervention programs require the use of high-quality measures that evaluate child abuse potential. Researchers frequently use the Child Abuse Potential Inventory (Milner, 1986) and the Brief Child Abuse Potential Inventory (BCAP; Ondersma et al., 2005) which contain Validity indices that evaluate response patterns such as social desirability responding. The measure developers recommended that abuse risk scores from invalid profiles be disregarded in clinical and research settings; however, little is known about factors that may relate to invalid responses and whether …

Synthetic Elaboration Of Β-Carbonyl Alkylboronic Esters, Mason D. Hamilton Ph.D.

Graduate Theses, Dissertations, and Problem Reports (ETD)

Organoboron compounds are some of the most synthetically versatile compounds in organic chemistry due to the many valuable transformations of the C-B bond. This synthetic versatility combined with the pharmacophoric nature of carboxylic acids has led to an increased interest in the one-pot difunctionalization of vinyl arenes using CO₂ and pinacol boranes. Recently, much progress has been made to improve the scope and versatility of boracarboxylation reactions to now include electron-deficient and α-methyl substituted vinyl arenes. However, the potential transformations of boracarboxylated products have remained unexplored. Here, methodologies to transform the β-aryl alkylboronic ester into new C-C, C-N, and …

Sex, Drugs, And Tbi: The Effects Of Sex Hormones And Trkb Activation On Alcohol-Related Behavior Following A Traumatic Brain Injury, Robin B. Oliverio

Graduate Theses, Dissertations, and Problem Reports (ETD)

Traumatic brain injury (TBI) and alcohol use disorder (AUD) exhibit a bidirectional relationship: AUD increases the risk of incurring a TBI and, inversely, a history of TBI increases the risk of future AUD. The second portion of this relationship has not been as thoroughly investigated. Our lab previously demonstrated that a juvenile TBI significantly increases alcohol self-administration in females compared to uninjured females and the injured females developed a conditioned place preference (CPP) for ethanol. Given this stark sex difference, this dissertation will examine the mechanism through which this occurred by investigating how manipulations of sex steroids might affect this …

Cell-Type-Specific Effects Of Synaptic Zinc In Mouse Auditory Cortex, Mason Mccollum

Graduate Theses, Dissertations, and Problem Reports (ETD)

Synaptic zinc signaling modulates synaptic activity and is present in specific populations of cortical neurons, suggesting that synaptic zinc contributes to the diversity of intracortical synaptic microcircuits and their auditory tuning properties. Stimulus-specific adaptation is a hallmark of sensory processing in which a repeated stimulus results in diminished successive neuronal responses, but a deviant stimulus will still elicit robust responses from the same neurons. Recent work has established that synaptically released zinc is an endogenous mechanism that shapes neuronal responses to sounds in the auditory cortex. Here, to understand the role of zinc signaling in the auditory cortex and the …

Characterizing Prescribed Fire With Terrestrial Lidar In The New Jersey Pine Barrens, Samuel Rhule Stockton

Graduate Theses, Dissertations, and Problem Reports (ETD)

Prescribed burning has become a commonly used tool in the mitigation of wildfire, though monitoring the way it changes ecosystems has historically been a time-intensive process. Rapid change across ecosystems has necessitated advancements in remote sensing technologies to quantify the changes taking place. Single-scan terrestrial LiDAR scanning is one such method of monitoring these changes through the quantification of ecosystem structural characteristics. Acute disturbance events such as fire can transform the structure of an ecosystem, and by extension, change the way that ecosystem functions. The purpose of this study is to analyze the changes in vegetation density and distribution following …