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Graduate Theses, Dissertations, and Problem Reports (ETD)

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The Origin And Properties Of Ionized Gas In The Inner Milky Way Disk, Dylan J. Linville Jan 2025

The Origin And Properties Of Ionized Gas In The Inner Milky Way Disk, Dylan J. Linville

Graduate Theses, Dissertations, and Problem Reports (ETD)

The interstellar medium (ISM) plays a crucial role in the evolution of the Milky Way and other spiral galaxies, but numerous gaps remain in our collective understanding of its properties. The now-complete Green Bank Telescope (GBT) Diffuse Ionized Gas Survey (GDIGS) and the ongoing extension, the GBT Diffuse Ionized Gas Survey at Low Frequencies (GDIGS-Low), offer the opportunity to study the ionized component of the ISM at unmatched sensitivity and resolution. In this dissertation I will utilize GDIGS and GDIGS-Low data to improve our understanding of discrete regions of ionized gas. I first use GDIGS to provide improved measurements of …


Impact Of Whey And Soy Protein Supplementation On Resistance Training In Young Adults: A Systematic Literature Review And Meta-Analysis, Brandon Eric Davis Jan 2025

Impact Of Whey And Soy Protein Supplementation On Resistance Training In Young Adults: A Systematic Literature Review And Meta-Analysis, Brandon Eric Davis

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Whey protein is a popular supplement in sports nutrition. Soy protein is an alternative to whey protein, often marketed to individuals following a plant-based diet. Evaluating whey and soy protein supplementation's role in resistance exercise is essential for providing evidence-based nutrition guidance.

Objective: The study aim was to conduct a systematic literature review and meta-analysis on randomized control trials (RCTs) on the effectiveness of whey and soy protein supplementation on outcomes of lean body mass (LBM), squat, bench press, and plasma essential plasma amino acid (EAA) levels in young adult men and women.

Methods: The databases …


Manualized Interventions For Individuals Experiencing Or At-Risk For Adverse Life Experiences, Sierra P. Mcnicol Jan 2025

Manualized Interventions For Individuals Experiencing Or At-Risk For Adverse Life Experiences, Sierra P. Mcnicol

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Occupational practitioners have a role in providing holistic and client-centered care to promote occupational performance, engagement, and satisfaction through occupations. Many individuals are impacted in the engagement of occupations secondary to adverse life experiences that have caused mental distress, difficulties in cognitive processing, limited social support, and disruptions in social determinants that have made accessing services complicated. Purpose: Due to the increased likelihood of individuals who have experienced adverse life experiences experiencing occupational distress and performance limitations, the capstone student aimed to develop an understanding of adverse life experiences, provide insight into the needs of the population, and develop …


Effectiveness Of A Cumulative Pediatric-Based Practical Examination At Increasing Student Perceived Competence And Confidence In Working With Pediatric Populations, Nicholas J. Passarelli Jan 2025

Effectiveness Of A Cumulative Pediatric-Based Practical Examination At Increasing Student Perceived Competence And Confidence In Working With Pediatric Populations, Nicholas J. Passarelli

Graduate Theses, Dissertations, and Problem Reports (ETD)

Current literature highlights the importance of utilizing practical examinations and actual patients and covering vast pediatric content within occupational therapy curricula. The needs assessment conducted within The West Virginia University Division of Occupational Therapy revealed that the division currently employs all three of these factors within their curriculum, however a gap presented in the combination of these three factors together. This project aimed to diminish this gap through the development, implementation, and execution of a pediatric practical examination, utilizing actual pediatric patients, to determine whether the examination is effective in increasing student perceived competence and confidence in working with pediatric …


Experimental And Finite Element Analysis Of Vacuum Infused Frp Composite Π-Joints, Jared Cameron Short Jan 2025

Experimental And Finite Element Analysis Of Vacuum Infused Frp Composite Π-Joints, Jared Cameron Short

Graduate Theses, Dissertations, and Problem Reports (ETD)

Composite π-joints are valued for their potential to reduce component weight, lower manufacturing costs, and simplify assembly. In aerospace applications, these qualities become even more valuable; however, these advantages must be carefully balanced with the safety requirements. Given the complexity of the π-joint geometry, it is crucial to determine and optimize the mechanical properties to ensure quality, performance, and safety throughout the lifetime of the part.

This thesis aims to investigate the effects of various factors on the mechanical properties of a composite π-joint manufactured using vacuum-assisted resin transfer molding (VARTM) processes. The discussion focuses on the effects of fabric …


Intersectional Embodiment: An Exploration Of Women's Bodily Experiences In Contemporary American Literature, Olivia Marie Wertz Jan 2025

Intersectional Embodiment: An Exploration Of Women's Bodily Experiences In Contemporary American Literature, Olivia Marie Wertz

Graduate Theses, Dissertations, and Problem Reports (ETD)

My dissertation, “Intersectional Embodiment: An Exploration of Women’s Bodily Experiences in Contemporary American Literature,” examines contemporary American novels written by women of color in order to illuminate how women of color protagonists realize, protect, and embrace their bodies as a source of power in a deeply oppressive society. Specifically, I analyze Kindred (1979) and the Parable novels (1993, 1998) by Octavia Butler, Displacement (2019), a graphic novel by Kiku Hughes, and Severance (2018) by Ling Ma. In these novels, women protagonists must account for their bodies to understand their identity, combat oppressive bodily control, and, above all, survive. Both neo-historical …


Not Everyone Believes Sports Are A Safe Space: Development Of A Toxic Sports Fandom Scale, Thomas Dean Bobbitt Jan 2025

Not Everyone Believes Sports Are A Safe Space: Development Of A Toxic Sports Fandom Scale, Thomas Dean Bobbitt

Graduate Theses, Dissertations, and Problem Reports (ETD)

Dysfunctional Fan Behavior is a concept developed by Wakefield & Wann (2006), that describes fan conduct, consisting of complaining and confrontation, that ruins the experience of sporting events for others. However, there is some evidence that although the items featured in the Dysfunctional Fan Behavior Scale are perceived as negative, they are also viewed as relatively normal and appropriate of sports fans. Bobbitt et al.’s (in press) qualitative analysis suggests that these behaviors are not always considered destructive or toxic in the context of sport, but the line between benignly rude and genuinely antisocial behavior is somewhat difficult to define. …


Microwave-Assisted Reduction Of Critical Metal Oxides From E-Waste Mixture, Kurundu Shavinka Jayasekera Jan 2025

Microwave-Assisted Reduction Of Critical Metal Oxides From E-Waste Mixture, Kurundu Shavinka Jayasekera

Graduate Theses, Dissertations, and Problem Reports (ETD)

The efficient recovery of critical metals such as tantalum (Ta), manganese (Mn), gallium (Ga), and indium (In) from electronic waste (e-waste) is essential for resource sustainability and environmental protection. This research uses microwave-assisted treatment to investigate the carbothermal reduction of these four specific metals from their oxides in a simulated e-waste mixture, with carbon black serving as both a microwave coupling and reduction agent. Microwave processing is used specifically because it offers a rapid and energy-efficient alternative to conventional thermal methods, with the benefits of intrinsic heating and selective heating of materials. The first experiments centered on understanding the effects …


No Sweeter Sound: Musicking, Transformative Pedagogy, And Collaborative Social Justice In Choral Settings, Natalie N. Shaffer Jan 2025

No Sweeter Sound: Musicking, Transformative Pedagogy, And Collaborative Social Justice In Choral Settings, Natalie N. Shaffer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Choral music and its conductors occupy a tenuous space in the 21st century. In a new cultural atmosphere influenced by Gen Z’s expectation of welcomed diversity and intolerance for injustice, TikTok’s rapid global dissemination of information and entertainment, and society’s desire for authentic connection following the Covid-19 pandemic, the dichotomies present in choral music are no longer being glossed over. Difficult conversations about race, gender, ableism, social location, and other intersectional identifiers are at the forefront of academic writing and conference presentations. Evidence of transformative pedagogy and culturally responsive practices are beginning to appear in classroom syllabi and rehearsal techniques …


Chopin’S Innovation And Influence On The French Piano School: From His Predecessors To His Principal Students And Successors, Wenjun Xia Jan 2025

Chopin’S Innovation And Influence On The French Piano School: From His Predecessors To His Principal Students And Successors, Wenjun Xia

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chopin’s Innovation and Influence on the French Piano School: From his Predecessors to his Principal Students and Successors

Wenjun Xia

This research explores Frédéric Chopin's (1810–1849) innovations in piano technique, pedagogy, composition, and interpretation, as well as Chopin’s subsequent influence on the French piano school. Before Chopin’s arrival in Paris, the French piano school was primarily influenced by Louis Adam (1758–1848) and Friedrich Kalkbrenner (1785–1849), whose approaches dominated piano pedagogy at the Paris Conservatoire. Early French piano pedagogy centered on finger precision, virtuosity, and clarity, but expressivity was somewhat limited. Chopin initiated Romantic trends that emphasized emotion and natural physical …


Commissioning And Recording Project Of New Works For Saxophone Focused On Promoting Mexican Composers And Educating Future Commissioning Saxophonists, Andres Efrain Santana Jan 2025

Commissioning And Recording Project Of New Works For Saxophone Focused On Promoting Mexican Composers And Educating Future Commissioning Saxophonists, Andres Efrain Santana

Graduate Theses, Dissertations, and Problem Reports (ETD)

This document chronicles my process of commissioning and recording five new works by Mexican composers. To contextualize this experience, I provide a brief introduction of Mexican contemporary music history, an overview of second-hand accounts of the commissioning process, my personal experience of this process, and my assessment of the impact these works could have on the classical saxophone repertoire. As I have reflected on my own experience with historical and contemporary accounts of the commissioning process, it has become clear that personal connections between composers and performers and the patronage or funding linked to those relationships are just as important …


Xanthine Oxidoreductase, Uric Acid, And Iron Homeostasis: Discovering A Therapeutic Vulnerability In Breast Cancer, Matthew G. Chapa Jan 2025

Xanthine Oxidoreductase, Uric Acid, And Iron Homeostasis: Discovering A Therapeutic Vulnerability In Breast Cancer, Matthew G. Chapa

Graduate Theses, Dissertations, and Problem Reports (ETD)

Breast cancer remains the most frequently diagnosed malignancy and the leading cause of cancer related deaths in women. This is primarily due to distant metastases, which highlight the necessity to understand factors that contribute to breast cancer progression. Emerging evidence associates decreased tumor xanthine oxidoreductase (XOR) expression with a poorer prognosis, increased recurrence, and a more aggressive phenotype. While healthy liver, intestines, kidneys, and breast tissue have robust XOR expression and activity, there is a tendency for this to be ablated in carcinogenesis. This dissertation investigates XOR's role in breast cancer and identifies its product, uric acid (UA), as possessing …


Adolescent Attachment And Anxiety: Examining Implicit Emotion Beliefs And Emotion Regulation As Mediators, Jeanette Pool Jan 2025

Adolescent Attachment And Anxiety: Examining Implicit Emotion Beliefs And Emotion Regulation As Mediators, Jeanette Pool

Graduate Theses, Dissertations, and Problem Reports (ETD)

Anxiety is a growing issue in adolescents, with teens reporting worse and more severe symptoms in recent years (Kajastus et al., 2023; Solmi et al., 2022). There are several risk factors for developing anxiety symptoms, including cognitive, behavioral, and social factors. Effective emotion regulation (ER) has been shown to be helpful in decreasing anxiety symptom levels (Arbulu et al., 2023; Cho et al., 2019). A better understanding of precursors of ER could aid in prevention of anxiety. ER may stem from our personal beliefs about emotions (Ford et al., 2018; Schroder et al., 2015; Tamir et al., 2007) and to …


La2nio4+Δ-Based Solid Oxide Electrolysis Cell (Soecs) Electrodes Enhanced With Complex Perovskite Nanocatalyst Processed By Surfactant-Enabled Infiltration, Cole Samuel Klemstine Jan 2025

La2nio4+Δ-Based Solid Oxide Electrolysis Cell (Soecs) Electrodes Enhanced With Complex Perovskite Nanocatalyst Processed By Surfactant-Enabled Infiltration, Cole Samuel Klemstine

Graduate Theses, Dissertations, and Problem Reports (ETD)

As the world seeks to reduce its reliance on hydrocarbons, the demand for sustainable hydrogen production methods has become increasingly critical. Hydrogen is a versatile energy carrier that can be produced from various sources, including water, natural gas, and biomass. Replacing the burning of hydrocarbons with hydrogen could significantly reduce greenhouse gas emissions, contributing to global climate goals like those set by the US H2NEW program. The joint technologies of solid oxide fuel and electrolysis cells present the ability to both produce and process clean hydrogen to meet these goals. Development into solid oxide fuel cells has been met with …


Reassessing Hourly Neurological Assessments: A Quality Improvement Initiative, Haley Lynne Johnson Jan 2025

Reassessing Hourly Neurological Assessments: A Quality Improvement Initiative, Haley Lynne Johnson

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Reassessing Hourly Neurological Assessments: A Quality Improvement Initiative

Haley Lynne Johnson, BSN, RN

Background: Stroke and brain injuries remain among the leading causes of mortality in the United States, accounting for 162,890 deaths in 2021 (Centers for Disease Control and Prevention [CDC], 2021). Although frequent neurological assessments are essential for monitoring patient status, limited evidence supports the prolonged use of hourly neuro-checks (LaBuzetta et al., 2022). Excessive neuro-check frequency has been linked to sleep deprivation, an increased risk of delirium, and inefficient resource utilization (Kishore et al., 2021).

Purpose: This project aimed to assess the impact of …


Apostrophes And Archimedes, Evan J. Clough Jan 2025

Apostrophes And Archimedes, Evan J. Clough

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis explores how digital collage and printmaking can be used to develop images through acts of subjective response, intentional and unintentional acts of appropriation, and parodic re-use and comparison pushed to absurd extremes. It looks at the history of creating art for parodic re-use and examines “Pataphysics”, developed by and for art appropriation of pattern, form, and process. These concepts are explained and related to visual artwork that was made for the thesis exhibition Apostrophes and Archimedes Cast Shadow Puppets. The exhibition invites viewers to experience the phenomenon of observation, automatic transformation, and creation using multiple modalities of exaggerated …


Soft Epilogue, Chloe C. Firth Jan 2025

Soft Epilogue, Chloe C. Firth

Graduate Theses, Dissertations, and Problem Reports (ETD)

This written document is the accompanying thesis for my Master of Fine Arts Exhibition, Soft Epilogue. The work in the exhibition serves as a visual record of my eating disorder recovery process. Each piece in the exhibition addresses a selected writing prompt from the book 8 Keys to Recovery from an Eating Disorder, published in 2011 and written by therapists and former eating disorder patients Carolyn Costin and Gwen Schubert Grabb. The book provides information and journal assignments regarding the development of eating disorders, how to maintain the motivation to recover, and how to navigate the complicated emotions …


Molecular Characterization Of Fungus-Arthropod Associations, Angie M. Martin Jan 2025

Molecular Characterization Of Fungus-Arthropod Associations, Angie M. Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Arthropods, or phylum Arthropoda, are the largest group of animals, accounting for over three quarters of all animal records on NCBI Taxonomy. And yet, only 40% of all deposited sequences on NCBI Nucleotide come from arthropods. Clearly, arthropods are an understudied group of animals: they are small, often drab, and lead lives very alien to our own. Arthropods have chitinous exoskeletons, segmented bodies, and jointed appendages, and include crabs, barnacles, insects, spiders, and millipedes. Since the era of DNA based studies took off in the 1980s, DNA and other small molecules have been integral to our understanding of the Earth …


Evaluating Osmia Cornifrons As An Effective Pollinator For Agricultural Crops: An Analysis Of Hair Morphology And Sex-Ratio Distorting Bacterium, Wolbachia, Nellie Heitzman Jan 2025

Evaluating Osmia Cornifrons As An Effective Pollinator For Agricultural Crops: An Analysis Of Hair Morphology And Sex-Ratio Distorting Bacterium, Wolbachia, Nellie Heitzman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Effective pollination relies not only on the presence of pollinators but also on their morphological adaptations and reproductive biology. In this study, I investigated both the external hair morphology and the intracellular bacterial infection status of Osmia cornifrons, a solitary bee species increasingly used in orchard pollination due to its efficiency and early seasonal activity. Using Scanning Electron Microscopy (SEM), I characterized the distribution and structure of two major hair types present on the bee's body: branched and unbranched. Branched hairs were found widely distributed across the head, thorax, dorsal abdomen, and legs, and appeared to function primarily in incidental …


Authoritarian Control Over Election Administration, Nazar Boyko Jan 2025

Authoritarian Control Over Election Administration, Nazar Boyko

Graduate Theses, Dissertations, and Problem Reports (ETD)

Street-level election bureaucracy (SLEB) remains one of the least studied aspects of authoritarian elections. Our current understanding of micro-level election administration in autocracies is fragmented and unsystematic. In this dissertation, I develop and test a theory of authoritarian SLEB that comprehensively addresses key elements of election administration: the incentivization and mobilization of election commissioners, the establishment of control over election commissions, the monitoring of their performance, and the post-election reshaping of their personnel composition. Drawing on rational choice theory, I propose a framework in which autocrats are seen as outcome-maximizers who strategically design SLEB to achieve their electoral goals. The …


Global China In Pakistan: Mixed-Methods Analysis Of Symbolic Domination, Social Media Geopolitics, And Security Corridors, Davide Giacomo Zoppolato Jan 2025

Global China In Pakistan: Mixed-Methods Analysis Of Symbolic Domination, Social Media Geopolitics, And Security Corridors, Davide Giacomo Zoppolato

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores the discursive and material dimensions of China’s global rise through a case study of the Belt and Road Initiative (BRI) in Pakistan. As China becomes a leading provider of development finance, its infrastructure-led approach is reshaping geographies across the Global South. Focusing on the China–Pakistan Economic Corridor (CPEC)—the BRI’s flagship project—I examine how Chinese and Pakistani actors co-produce development through discourses, infrastructures, and security regimes. Structured around three articles, the dissertation analyzes: (1) the official diplomatic discourses of Chinese state institutions and their reception by Pakistani media outlets; (2) the curated geopolitical discourses of Chinese social media …


Introduction Of Polar Functional Groups To Pyridinedipyrrolide Zirconium Photosensitizers Through Post-Synthetic Modifications, Marisa Nicole Tordella Jan 2025

Introduction Of Polar Functional Groups To Pyridinedipyrrolide Zirconium Photosensitizers Through Post-Synthetic Modifications, Marisa Nicole Tordella

Graduate Theses, Dissertations, and Problem Reports (ETD)

Photosensitizers are vital components to applications such as photoredox catalysis, photodynamic therapy, and various avenues of solar energy production. The abilities of transition metal photosensitizers containing precious metals have been well studied over the years. However, because of the growing scarcity of these metals, there has been a shift in finding more earth abundant alternatives. To help fill this gap in the literature, the Milsmann group has developed and studied the photophysical properties of Zr(IV) complexes with various ligand systems. Due to the electron deficient metal center, these complexes exhibit ligand-to-metal charge transfer (LMCT), unlike the metal-to-ligand charge transfer commonly …


Media Framing Of The Local And National News Coverage Of The East Palestine Train Derailment, Lauren Delo Jan 2025

Media Framing Of The Local And National News Coverage Of The East Palestine Train Derailment, Lauren Delo

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research examined digital news coverage of the East Palestine train derailment to determine the frames and dominant frames used in both local and national coverage of the disaster, and whether these frames differed over time. Specifically, this research used a content analysis of digital newspaper articles from five media outlets—three national level and two local level—and applied the frames identified from Colistra’s (2010) study of print news coverage of the 1972 Buffalo Creek mine disaster. Results revealed significant differences between the most frequently used frames between local and national coverage, shifts in frame usage over time within each media …


Investigating The Physiological, Metabolomic, And Transcriptomic Changes In Response To Telomerase Deletion In Saccharomyces Cerevisiae, Taizina Momtareen Jan 2025

Investigating The Physiological, Metabolomic, And Transcriptomic Changes In Response To Telomerase Deletion In Saccharomyces Cerevisiae, Taizina Momtareen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Telomerase is an enzyme found in rapidly dividing cells that maintains chromosome ends and prevents the loss of genetic information during cell division. When telomerase is inactive, cells gradually lose their telomeres and eventually stop dividing. In yeast, a rare subset of cells can bypass this growth arrest by switching to a recombination-based mechanism to maintain telomeres. Therefore, yeast provides a powerful system to study this adaptation, as telomerase-deficient strains can generate 'survivors' that mimic the Alternative Lengthening of Telomeres (ALT) pathway observed in some human tumors. In this work, my goal was to examine how a telomerase-null (TLC1 …


“Situation Is Out Of Control:” Drug Use, Violence, Resistance, And Politics During The Vietnam War, Andrew Domenic Ray Jan 2025

“Situation Is Out Of Control:” Drug Use, Violence, Resistance, And Politics During The Vietnam War, Andrew Domenic Ray

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Vietnam War was notable because it was a very violent and very public war. Throughout the course of the conflict, the United States imposed anti-guerilla warfare on a grand scale. American soldiers were often given orders to destroy the enemy at all costs; but in a war with no discernable front lines, the difference between combatant and non-combatant was, at best, blurry. The way the war was being fought, coupled with its unpopularity and emerging counterculture at home, made the Vietnam War a hub for illicit drug use among American soldiers. American soldiers use drugs for a number of …


Determination Of Spatial Origin Of Striations On Fired Projectiles, Kaitlin A. Dolan Jan 2025

Determination Of Spatial Origin Of Striations On Fired Projectiles, Kaitlin A. Dolan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Firearms examination has been a mainstay of Forensic Science for over a century. One of the principal assessments performed by firearm examiners is the comparison of fired projectiles to a suspect firearm. The AFTE theory of identification, as it pertains to firearms and toolmarks, supports the basis that the barrel of a firearm will impart class and individualizing characteristics onto the fired projectile. The characteristics are developed by the interaction of the projectile surface with the lands of the barrel. Features on the lands, which are incidental to the process of manufacturing, dictate the characteristics observed on the fired projectile. …


Asset Mapping: A Tool Used To Identify Community Resources, Gentry Shrewsbury Jan 2025

Asset Mapping: A Tool Used To Identify Community Resources, Gentry Shrewsbury

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Physical activity is vital for health and quality of life; however, for individuals with disabilities, there are numerous barriers to participation. In rural and low-socioeconomic regions, barriers such as lack of awareness, limited restrooms, and lack of public transportation create challenges when attempting to be physically active. Using the Asset-Based Community Development (ABCD) framework, this study examined physical activity resources within Mercer County, West Virginia, and evaluated their accessibility for individuals with disabilities.

Methods: A qualitative research design was implemented using three data collection strategies: (1) a google search to identify existing physical activity opportunities followed by a windshield …


Nuclear Reactions: Making Sense Of The Accident At Three Mile Island, Jordan Emily Bennett Jan 2025

Nuclear Reactions: Making Sense Of The Accident At Three Mile Island, Jordan Emily Bennett

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis examines the causes and consequences of the nuclear accident at Three Mile Island in March of 1979. First detailing the event, this thesis then examines activist narratives following the accident, with a particular focus on women’s “motherist” activism in the community outside the plant, before turning to questions of memory and the creation of a public memory of survivorship in the community surrounding the plant. In considering these questions, this thesis seeks to shed light on the differences between official and communal narratives of the accident in an effort to understand the impacts that the partial meltdown at …


Revitalizing The Urban Riverfront: Redesigning The West End Bridge In Pittsburgh,Pa For Sustainable Multi-Use Connectivity And Public Space Creation., Mina Kargari Rezapour Jan 2025

Revitalizing The Urban Riverfront: Redesigning The West End Bridge In Pittsburgh,Pa For Sustainable Multi-Use Connectivity And Public Space Creation., Mina Kargari Rezapour

Graduate Theses, Dissertations, and Problem Reports (ETD)

Urban waterfronts are critical assets for cities, offering opportunities for ecological restoration, public engagement, and multi-modal transportation. However, many riverfronts remain underutilized due to infrastructure barriers and lack of accessibility.

Pittsburgh’s riverfront is dynamic asset that supports recreation, cultural expression, and community interaction. The Three River Heritage Trail Loop, a visionary project led by River Life over the past 20 years, seeks to connect diverse neighborhoods and natural assets along the city’s rivers. At the heart of this initiative is the West End Bridge, serving as the bookend and a pivotal gateway to complete the loop and critical link for …


Evaluation And Identification Of Novel Viruses In Wild Fishes Using Varied Molecular Biology Techniques, Clayton Douglas Raines Jan 2025

Evaluation And Identification Of Novel Viruses In Wild Fishes Using Varied Molecular Biology Techniques, Clayton Douglas Raines

Graduate Theses, Dissertations, and Problem Reports (ETD)

Viruses are among the most abundant biological entities on Earth, yet their diversity and ecological roles in aquatic vertebrates, particularly fishes, remain poorly characterized. This dissertation advances fish virology through integrative approaches that combine historical context, molecular diagnostics, and genomic discovery. A novel hepadnavirus (ApHBV) was identified in asymptomatic Alosa pseudoharengus, emphasizing the prevalence of subclinical infections in migratory forage species. In Catostomus commersonii, transcriptomic profiling revealed sexually dimorphic immune responses to hepatitis B and C-like viruses, highlighting complex host-pathogen dynamics. In Micropterus nigricans, next-generation sequencing uncovered a novel nackednavirus and multiple adomaviruses within hyperpigmented lesions, expanding …