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Graduate Theses, Dissertations, and Problem Reports (ETD)

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Exploring The Use Of Human Metrology For Biometric Recognition, Nikhil Mallikarjun Reddy Burri Dec 2007

Exploring The Use Of Human Metrology For Biometric Recognition, Nikhil Mallikarjun Reddy Burri

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis explores the possibility of incorporating human body measurements in a biometric framework. While metrological features have been used for identifying persons in the late 19th century, there is limited work in automating this process for surveillance applications. We first establish the relevance of using metrological features in biometric systems by studying two anthropometric data-sets (NASA and NHANES). We then propose a technique to automatically extract a subset of these measurements from a video sequence. A robust segmentation technique (HMMF) to detect moving pixels corresponding to human objects is used in the first stage. Next, we use Active Contours …


Experimental Studies Of Electromagnetic Signals To Enhance Radio Imaging Method (Rim), William D. Monaghan Dec 2007

Experimental Studies Of Electromagnetic Signals To Enhance Radio Imaging Method (Rim), William D. Monaghan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Safety and production of coal mining operations could be greatly affected by geologic anomalies such as faults, sandstone intrusions, dykes, fracture zones, sudden thinning and severe undulation of the coal seam. Since most of these geologic anomalies in coal seams effect the attenuation rate of electromagnetic signal that passes through them, the radio imaging method (RIM), using a signal in the kilohertz range, is capable of locating the zones of geologic anomalies in underground coal mines with the help of tomographic reconstruction programs. RIM technology is a promising geophysical tool for exploring the geologic anomalies ahead of modern longwall faces, …


Creating A Gis Database For Nursing Home Evacuation Planning In Virginia Beach, Virginia, Randall S. Dawson Dec 2007

Creating A Gis Database For Nursing Home Evacuation Planning In Virginia Beach, Virginia, Randall S. Dawson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The focus of this project is planning for the evacuation of the nursing homes of the city of Virginia Beach, VA in response to a hurricane risk. The population of nursing homes, hospitals and assisted living communities need special assistance when evacuating an area, requiring medications, equipment, and specialized transportation. Previous studies for Virginia Beach have provided generalizations for the population as a whole yet have provided very little data for specific communities such as nursing homes. A GIS database is created for nursing home establishments including road networks, bridges and tunnels, elevation, estimated facility population, and recently modeled storm …


Accessibility Of Wvu Websites For Individuals With Vision Impairments, Sarah Jacobin Dec 2007

Accessibility Of Wvu Websites For Individuals With Vision Impairments, Sarah Jacobin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recently, several major corporations have been sued because their websites are inaccessible to individuals with disabilities. For a website to be accessible, people with disabilities should be able to navigate and interact with the site. Individuals who are blind or have vision impairments have difficulty accessing websites because they commonly use assistive technology to interpret content. This study is the first comprehensive study assessing the accessibility of WVU websites for individuals with disabilities, specifically individuals with visual impairments and comparing those results to those of two comparable universities in other areas of the country. Compliance with specifications of website accessibility …


Application Of The Emotional Intelligence Construct To College Student Binge Drinking, Jeffrey P. Dulko Dec 2007

Application Of The Emotional Intelligence Construct To College Student Binge Drinking, Jeffrey P. Dulko

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although extensive research has been conducted in the area of college binge drinking, relatively little has been done to study differences among alcohol-related consequences. Some initial studies have shown that binge drinking and its consequences are associated with several emotional and interpersonal factors, but there has been no comprehensive examination of these relationships. The theory of emotional intelligence (EI) has been developed recently to study emotional and interpersonal factors as an integrated construct. Research of the relationship between alcohol and EI could provide a valuable base for which to design alcohol prevention programs that target improving emotional and interpersonal deficits …


Design And Real-Time Control Of Shipboard Power System Testbed, Pradeep Pant Dec 2007

Design And Real-Time Control Of Shipboard Power System Testbed, Pradeep Pant

Graduate Theses, Dissertations, and Problem Reports (ETD)

The objective of this thesis is to design and test a small scale testbed for the all-electric shipboard power distribution system. Shipboard power system is increasingly becoming more reconfigurable, and multi-agent systems are developed to automate routine operation and emergency reconfiguration. Underlying algorithms of these systems have been verified using software simulation tools. However, these simulators run in soft realtime by using simple mathematical models to represent the physical system. These models do not incorporate every aspect of the physical system. A testbed provides a cost effective physical environment to verify these algorithms and control techniques in the real world. …


Relationship Between Food Insecurity And Overweight In Preschool-Aged Children In Rural West Virginia, Melissa Webber Aug 2007

Relationship Between Food Insecurity And Overweight In Preschool-Aged Children In Rural West Virginia, Melissa Webber

Graduate Theses, Dissertations, and Problem Reports (ETD)

The prevalence of overweight among adults and children continues to increase. The purpose of this study was to evaluate demographic factors that may be associated with an increase in a child's weight status. Subjects were children aged 2 to 6 years old in two southern West Virginia counties, McDowell and Mercer. Children were weighed and measured, and BMI was calculated. Parents or other caregivers were asked to complete a questionnaire regarding child's age, household food security, and parents' age, height and weight, education level, and hours worked per week.;A positive association between food insecurity and overweight among children was present …


Teacher Perceptions Of The Effectiveness Of The Social Studies Teacher Training Program At Tabouk Teachers' College In Saudi Arabia, Awad Hamad Alhwiti Aug 2007

Teacher Perceptions Of The Effectiveness Of The Social Studies Teacher Training Program At Tabouk Teachers' College In Saudi Arabia, Awad Hamad Alhwiti

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of the study was to examine and evaluate the effectiveness of the social studies teacher training program in Tabouk Teachers' College in Saudi Arabia. This research addressed six questions concerning teacher perceptions regarding the effectiveness of the teacher preparation programs in Tabouk Teachers' College. These questions examined teacher perceptions of the effectiveness of preparation they received for teaching in schools in Saudi Arabia. This study included teachers who completed student teaching and who graduated with a major in social studies. The 40-item instrument used to gather data included demographics, internship, instructor effectiveness, curriculum, teaching skills, classroom management, and …


Insulin-Induced Endothelial Cell Proliferation And Viability In Stretched Murine Skin And Cell Culture, Carl D. Shrader Jr. Aug 2007

Insulin-Induced Endothelial Cell Proliferation And Viability In Stretched Murine Skin And Cell Culture, Carl D. Shrader Jr.

Graduate Theses, Dissertations, and Problem Reports (ETD)

We developed a novel in vivo model utilizing acute stretch to investigate endothelial cell (EC) proliferation as a marker of vascular growth in healing SKH1 mouse skin. We also used human umbilical vein endothelial cells (HUVECs) as an in vitro model system to validate postulated tissue insulin-mediated signal transduction pathway(s) using paradigms that would prove lethal in the animal model.;Dorsal distally based flaps of skin were stretched for 3 min using linear (skin hook) plus hemispherical load cycling (inflated subcutaneous silicone catheter). Unstretched, wounded skin along the back and sternum served as postoperative controls. Laser Doppler flowmetry demonstrated a three-fold …


Utility Of The Structured Inventory Of Malingered Symptomatology (Sims) And The Assessment Of Depression Inventory (Adi) In Screening For Malingering Among Disability Seeking Outpatients, Carl B. Clegg Aug 2007

Utility Of The Structured Inventory Of Malingered Symptomatology (Sims) And The Assessment Of Depression Inventory (Adi) In Screening For Malingering Among Disability Seeking Outpatients, Carl B. Clegg

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sixty-four individuals undergoing a social security disability evaluation were administered the Structured Interview of Reported Symptoms (SIRS), Structured Inventory of Malingered Symptomatology (SIMS), and Assessment of Depression Inventory (ADI). Individual results were classified as honest or suspected malingering based on SIRS scores. In addition, 63 individuals from the community were randomly assigned to complete the SIMS and the ADI honestly or as if they were malingering depression. Both malingering groups had significantly higher mean scores on the SIMS Total and ADI Feigning scales than both honest groups. The scores of the malingering groups did not significantly differ. In the clinical …


The Effect Of Moisture Addition With A Mold Inhibitor On Feed Manufacture, Pellet Quality, And Broiler Performance, John M. Hott Aug 2007

The Effect Of Moisture Addition With A Mold Inhibitor On Feed Manufacture, Pellet Quality, And Broiler Performance, John M. Hott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Commercial broilers are typically fed pelleted diets because of the resulting improvements in bird performance and ease of transportation. Feed and feed manufacturing costs account for 60--70% of the total costs incurred in a broiler production system. Feed manufacturers are constantly searching for ways to decrease production costs while maintaining high pellet quality. Moisture addition at the mixer has been shown to increase pellet quality and decrease pellet mill energy consumption. However, past literature has incorporated high moisture addition levels that may be impractical in an industrial setting. The objective of this study was to determine the effects of moisture …


Increased Resistance Of Escherichia Coli O157:H7 To Electron Beam Following Repetitive Irradiation At Sub-Lethal Doses And An Analysis Of Genes Of Escherichia Coli Resistance, Leah Levanduski Aug 2007

Increased Resistance Of Escherichia Coli O157:H7 To Electron Beam Following Repetitive Irradiation At Sub-Lethal Doses And An Analysis Of Genes Of Escherichia Coli Resistance, Leah Levanduski

Graduate Theses, Dissertations, and Problem Reports (ETD)

One way that food processors in the United States control food-borne pathogens in a non-thermal manner is with the application of electron beam (e-beam) radiation. Increased resistance of Escherichia coli O157:H7 to various stressors such as pH, temperature, ionic strength, and antibiotics has been demonstrated; therefore, the objective of this study was to determine if the D10-value for E. coli O157:H7 ( E. coli) in ground beef increases due to repetitive exposure to e-beam at sub-lethal levels. Ground beef samples were inoculated with an ATCC strain of E. coli and incubated to approximately 109 CFU/g followed by e-beam processing. Survivors …


Integrating Participatory Gis And Political Ecology To Study Flood Vulnerability In The Limpopo Province Of South Africa, Nthaduleni S. Nethengwe Aug 2007

Integrating Participatory Gis And Political Ecology To Study Flood Vulnerability In The Limpopo Province Of South Africa, Nthaduleni S. Nethengwe

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation research examines differential flood vulnerability in the Thulamela municipality of the Limpopo Province of South Africa. The study employs a triangulation of methods within a Participatory GIS methodology. Political ecology is the conceptual framework. The main premise of this research is that household flood vulnerability is socially constructed in that it is mediated by locally contingent political, socio-economic and environmental factors. As a result, conventional solutions that focus mainly on the physical event are ineffective and costly because they ignore local knowledge about flood hazards and flood coping mechanisms that have been disrupted or destroyed. The study integrates …


Parametric Design Of A Coal Mine Refuge Chamber, Michael A. Fasouletos Aug 2007

Parametric Design Of A Coal Mine Refuge Chamber, Michael A. Fasouletos

Graduate Theses, Dissertations, and Problem Reports (ETD)

In 2006 several coal mine tragedies led to safety legislation to provide post accident protection to coal miners. One of the key aspects of this legislation was providing refuge areas where miners could await rescue if they could not escape the mine. These refuge areas were to be designed to sustain life for four days, providing the miners with adequate oxygen, carbon dioxide scrubbing, food, water, and reasonable temperature control. A parametric study has been performed to provide further definition of the requirements in order to design an optimal portable refuge chamber with little or no electricity to enhance the …


Pressure Deflection Behavior Of Candidate Materials For A Morphing Wing, Christopher Griffin Aug 2007

Pressure Deflection Behavior Of Candidate Materials For A Morphing Wing, Christopher Griffin

Graduate Theses, Dissertations, and Problem Reports (ETD)

The research presented in this thesis evaluates different materials that are candidates to be used as a flexible skin on a morphing wing. A morphing wing has come to be known as any wing that changes shape. Although there has been a great amount of research involved in designing morphing mechanisms, the information for applicable wing skin material has been limited. A morphing wing skin needs to be flexible enough to deflect and morph to a new shape given a suitable amount of force, yet strong enough to resist the aerodynamic forces encountered during flight. It also must remain elastic …


Validation Of A New Technique Of Production Data Analysis For Single And Multi-Layer Formations Under Controlled Environment, Domingo A. Mata Aug 2007

Validation Of A New Technique Of Production Data Analysis For Single And Multi-Layer Formations Under Controlled Environment, Domingo A. Mata

Graduate Theses, Dissertations, and Problem Reports (ETD)

Intelligent Production Data Analysis - IPDA, is a new methodology for Reservoir Characterization based only on monthly production rate data. This technique combines conventional methods of production data analysis (decline curve analysis, type curve matching and history matching) with intelligent systems. The study targets the validation of this methodology under a controlled environment, attempting three main objectives: Identifying Sweet Spots, Forecasting Reserves and recognizing under-performer wells.;The study investigates the behavior of five different reservoirs, modeled using a commercial simulator. The structure, parameters and heterogeneity of each configuration was inspired by existing formations. Records of production rate data were generated from …


Frequency Of Aeromonas Spp. Detection In Rainbow Trout And Recirculation Aquaculture Systems And The Storage Stability Of Fillets, Tabetha Marie Littler Aug 2007

Frequency Of Aeromonas Spp. Detection In Rainbow Trout And Recirculation Aquaculture Systems And The Storage Stability Of Fillets, Tabetha Marie Littler

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recirculating aquaculture systems enable increased fish production per volume of water. Unfortunately, recirculation systems also provide conditions for bacterial growth. Aeromonas, a genus of bacteria ubiquitous in the environment, contains fish pathogens, human pathogens, and/or spoilage organisms. Of the salmonid species, rainbow trout are the most susceptible to Motile Aeromonad Septicemia (MAS), the fish disease caused by Aeromonas spp. In humans, illnesses such as gastroenteritis, septicemia, and wound infections are caused by ingestion or exposure to Aeromonas spp. The objective of this study was to survey Aeromonas spp. present in the production system, on the fish, and on the butterfly …


Electronic Instrumentation Of Coal Slurry Impoundments For Real-Time Data Collection To Support Automated Monitoring, James A. Altobello May 2007

Electronic Instrumentation Of Coal Slurry Impoundments For Real-Time Data Collection To Support Automated Monitoring, James A. Altobello

Graduate Theses, Dissertations, and Problem Reports (ETD)

Following the breakthrough and release of coal slurry from the Martin County Coal Corporation impoundment near Inez, Kentucky on October 11, 2000 the United States Congress requested the National Research Council (NRC) to examine ways to reduce these types of accidents. The NRC completed their study titled "Coal Waste Impoundments" which identified numerous areas of concern and the committee presented recommendations for improving the design, operation, and safety of coal slurry impoundments. This research addresses the National Research Council's findings for introducing state-of-practice electronic instrumentation for monitoring parameters within the embankment and slurry pool area of an impoundment. This research …


Specifying Security Requirements Improvement For Ieee Standard 830, Jacob D. Mccarty May 2007

Specifying Security Requirements Improvement For Ieee Standard 830, Jacob D. Mccarty

Graduate Theses, Dissertations, and Problem Reports (ETD)

This paper presents a concept on how the software requirements specifications template provided by IEEE Standard 830 could be updated to ensure that security is analyzed during the early stages of the software development lifecycle. This improved security requirement in the software requirements specifications will ensure that software developers will have a more clear understanding of how to protect digital information.


Indian Warfare, Household Competency, And The Settlement Of The Western Virginia Frontier, 1749 To 1794, John M. Boback May 2007

Indian Warfare, Household Competency, And The Settlement Of The Western Virginia Frontier, 1749 To 1794, John M. Boback

Graduate Theses, Dissertations, and Problem Reports (ETD)

The thesis of this dissertation is that Indian-related violence and warfare had a profound influence on the duration and nature of the frontier experience of those men and women who settled in the western Virginia backcountry between 1749 and 1794. Recurrent attacks by Shawnees, Delawares, Mingos, and Indians from the Great Lakes region caused such widespread death, destruction, and depopulation that it effectively prolonged the period of austere and difficult living conditions for over forty years. This conclusion contradicts the assertions of some recent scholars who have argued that crude living conditions lasted for only a year or two on …


Epithelial Mechanisms In Airway Responses Induced By Hyperosmolarity, Yi Jing May 2007

Epithelial Mechanisms In Airway Responses Induced By Hyperosmolarity, Yi Jing

Graduate Theses, Dissertations, and Problem Reports (ETD)

There is airway dilation in normal people but obstruction in asthmatic patients during exercise; this has been thought to be initiated by hyperventilation causing evaporative water loss and hyperosmolarity of the airway surface liquid (ASL). In animal experiments the epithelium can detect osmolarity increases in the ASL and mediate relaxation of airway smooth muscle by releasing epithelium-derived relaxing factor (EpDRF), while in asthmatic patients this local regulatory function could be altered because of damage to epithelium. Challenged by hyperosmolarity, epithelial cells undergo shrinkage, volume-related ion transport, and release regulatory mediators, such as EpDRF. The changes in epithelial ion transport and …


A Grounded Theory Of Software Process Improvement Model Adoption, W. Grant Norman May 2007

A Grounded Theory Of Software Process Improvement Model Adoption, W. Grant Norman

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study, using a grounded theory methodology, analyzed data collected from software developers and IT professionals on software process improvement (SPI) adoption. The study is presented within a backdrop of organizational change steps described by John P. Kotter in his 1996 book, Leading Change. Software quality problems and failures have caused many financial losses, injuries, and even deaths. In the mid 1980s, as a means of mitigating these problems, the Department of Defense (DoD) and Carnegie Mellon University (CMU) began work on the Capability Maturity Model (CMMRTM) In 2001, the model was superseded by a more robust model, the Capability …


Tattooing And High-Risk Behavior Among Adolescents, Tiffany Lynn Stickel May 2007

Tattooing And High-Risk Behavior Among Adolescents, Tiffany Lynn Stickel

Graduate Theses, Dissertations, and Problem Reports (ETD)

High-risk behavior in the domains of tobacco, alcohol, and drug use, and sexual behavior were investigated in relation to tattoo presence/absence among adolescents aged 18-22 years. Adolescents' motivations to become tattooed were also explored. Participants included 400 tattooed and non-tattooed (147 male, 253 female) students at West Virginia University. Self-report questionnaires, which included items from the 2005 Youth Risk Behavior Surveillance System (YRBSS) as well as items derived by the investigator, were used to assess risk-taking behavior, tattoo presence/absence, and motives for obtaining/not obtaining tattoos. A significant relationship between high-risk behavior and tattooing was found using crosstabulation and chi-square analyses, …


Perceived Social Support Systems During Athletic Injury Recovery In Collegiate Club Sport Athletes, Jacqueline A. Loutsch May 2007

Perceived Social Support Systems During Athletic Injury Recovery In Collegiate Club Sport Athletes, Jacqueline A. Loutsch

Graduate Theses, Dissertations, and Problem Reports (ETD)

Context. Social support systems can be a key aspect in the overall health of the athlete, both physically and psychologically. It is important to be aware if an athlete is receiving the appropriate social support to aid them through their career. Objective. The purpose of this study is to determine which types of social support systems are used and who provides the different types of support. A secondary purpose is to investigate the types of social support that are perceived to be most important. Design. A prospective descriptive analysis about the influence of social support systems after an athlete is …


Comparison Of Two Electrofishing Gears (Backpack And Parallel Wires) And Abundances Of Fishes Of The Upper Greenbrier River Drainage, Angela D. Burns May 2007

Comparison Of Two Electrofishing Gears (Backpack And Parallel Wires) And Abundances Of Fishes Of The Upper Greenbrier River Drainage, Angela D. Burns

Graduate Theses, Dissertations, and Problem Reports (ETD)

The type of electrofishing gear influences capture efficiencies and abundance estimates of stream fishes. Few studies have examined the Holton and Sullivan (1954) parallel wires method of electrofishing. For this study, I modeled removal data with seven sampling occasions of three common species, western blacknose dace (Rhinichthys obtusus), fantail darter (Etheostoma flabellare), and mottled sculpin ( Cottus bairdi) from 10 paired sites in the upper Greenbrier River drainage, West Virginia, and estimated capture efficiencies of two electrofishing gear types (the Holton and Sullivan parallel wires method with alternating current and backpack units with pulsed-direct current). Ten candidate models represented alternative …


Cognitive Components, Information Search Processes, And Outcomes In A Decision Making Task, Beth A. Stafford May 2007

Cognitive Components, Information Search Processes, And Outcomes In A Decision Making Task, Beth A. Stafford

Graduate Theses, Dissertations, and Problem Reports (ETD)

Data from 49 younger adults with a mean age of 20.8 years old was used to examine the relations among cognitive abilities, information search strategies, and decision quality. Participants completed computerized relocation tasks for hypothetical individuals. A multiple linear regression was used to test the relations among the cognitive components fluid ability (Gf), crystallized ability (Gc), working memory (WM) and decision quality. A multiple linear regression was used to test the relations among the search strategies order of information searched, amount of information searched, search selectivity, and decision quality. A hierarchical regression was used to test the relations among cognitive …


Perceptions Of Calf Pool Participants Toward West Virginia Calf Pool Organizations, Carrie M. Stemple May 2007

Perceptions Of Calf Pool Participants Toward West Virginia Calf Pool Organizations, Carrie M. Stemple

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to determine perceptions of West Virginia calf pool producers with their local organization. This study was a descriptive survey research conducted with West Virginia calf pool producers who participated in a calf pool in 2005 and/or 2006. The majority of participants in the study reported that they were satisfied with the calf pool organizations, and would continue to participate. Over half reported that they planned to increase their production within five years in order to market more calves through the calf pool. The average calf pool producer in West Virginia is over 50 years …


Student Nagging Behavior In The College Classroom, Katie Neary Dunleavy May 2007

Student Nagging Behavior In The College Classroom, Katie Neary Dunleavy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nagging is a persuasive tactic yet to be fully explored in instructional communication. Nagging involves an exchange in which a student makes persistent requests of an instructor who fails to comply. The purpose of this dissertation was to examine nagging in the college classroom in order to ensure nagging was not just an aspect of other instructional concepts. The purpose was also to determine why students choose to nag, to examine the strategies students use to nag, and to examine the perceptions of students and instructors of these strategies. Finally, the purpose was also to examine nagging as a potentially …


Molecular Mechanisms Of Chromium(Vi)-Induced Apoptosis And Malignant Transformation, Neelam Azad May 2007

Molecular Mechanisms Of Chromium(Vi)-Induced Apoptosis And Malignant Transformation, Neelam Azad

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hexavalent chromium [Cr(VI)] compounds are redox cycling environmental carcinogens that induce apoptosis as the primary mode of cell death. Apoptosis is abnormally regulated in various disorders including cancer. Therefore, to understand the etiologies of these diseases, it is important to delineate the biochemical and molecular pathways involved in the regulation of apoptosis. The main objective of this study was to characterize the molecular mechanisms involved in Cr(VI)-induced apoptosis and malignant transformation. We found that both death receptor and mitochondrial pathways of apoptosis are involved in Cr(VI)-induced apoptosis, with the latter being more dominant. Consequently, overexpression of the mitochondrial anti-apoptotic protein …


Roles Of Luteal And Allantoic Function In Late Embryonic/Early Fetal Pregnancy Failures In Cattle, Justin D. Rhinehart May 2007

Roles Of Luteal And Allantoic Function In Late Embryonic/Early Fetal Pregnancy Failures In Cattle, Justin D. Rhinehart

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pregnancy failures during placentation in lactating dairy cows have been associated with low concentrations of peripheral serum progesterone (P 4) and poor development of the chorioallantoic membranes. Experiment I was done to determine if pregnant cows with high or low concentrations of serum P4 differed in luteal production of P4 and/or clearance of injected P4. Luteal tissue was removed from pregnant cows with ≥ 4.0 ng/mL (High) or ≤ 2.5 ng/mL (Low) serum P4 during d 28 to 34 post-insemination. Luteal tissue was assayed for P4 by radioimmunoassay and for expression of mRNA for preproendothelins 1 and 3, endothelin converting …