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Graduate Theses, Dissertations, and Problem Reports (ETD)

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Predicting Species Composition In An Eastern Hardwood Forest With The Use Of Digitally Derived Terrain Variables, Richard D. Flanigan May 2010

Predicting Species Composition In An Eastern Hardwood Forest With The Use Of Digitally Derived Terrain Variables, Richard D. Flanigan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis addresses the need for improved classification of remotely sensed imagery in the complex hardwood forests of West Virginia. A geographic information system (GIS) was used in conjunction with forest plot data to develop a model to predict species composition in the eastern hardwood forest of West Virginia. The study area was located on the West Virginia University Research Forest (WVURF) in northern West Virginia. Terrain variables including aspect, curvature and slope change drastically at a local scale within the forest to greatly influence species composition. Light Detection and Ranging (LiDAR) data was collected for the entire WVURF, which …

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The Effect Of Omega-3 Enhanced Fish Consumption On C-Reactive Protein Levels In Post-Menopausal Women, Megan Moran May 2010

The Effect Of Omega-3 Enhanced Fish Consumption On C-Reactive Protein Levels In Post-Menopausal Women, Megan Moran

Graduate Theses, Dissertations, and Problem Reports (ETD)

Heart disease is the leading killer of women in the United States, and the risk is 2--3 times greater in post-menopausal than pre-menopausal women. C-Reactive protein (CRP) is an inflammatory marker that has been identified as a risk factor for heart disease. The objective of this study was to determine if increased consumption of omega-3 fatty acids, known for their anti-inflammatory properties, reduced CRP levels in post-menopausal women. A sample of 29 women was recruited to participate in the study. Participants were randomly assigned to two groups, experimental and control. The women were instructed to follow a heart healthy diet …

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Evaluation Of Information Transfer Between Beef Producers And Extension Agents In West Virginia, Travis J. Cullen May 2010

Evaluation Of Information Transfer Between Beef Producers And Extension Agents In West Virginia, Travis J. Cullen

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to determine how information passes between beef producers and Extension Agents in West Virginia in the areas of teaching methods and program advertisement. A descriptive research design was used to collect the data for this study. The target population was all beef producers and the Agriculture and Natural Resource Extension Agents in West Virginia. The study found that beef producers and Extension Agents tend to be in agreement on both the methods of advertising and teaching. However, the programs beef producers desire differs from those the West Virginia University Extension Service offers.

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Polycystic Ovarian Syndrome: Proposal For An Evidence -Based Management Plan For An Electronic Patient Record System, Mary Elizabeth Durant May 2010

Polycystic Ovarian Syndrome: Proposal For An Evidence -Based Management Plan For An Electronic Patient Record System, Mary Elizabeth Durant

Graduate Theses, Dissertations, and Problem Reports (ETD)

The goal of this change project was to improve the diagnosis and management of women with polycystic ovarian syndrome (PCOS) through the use of an electronic patient record system. PCOS is one of the top ten missed or delayed diagnoses as well as an inconsistently or mismanaged women's health issue in primary care.;The chronic care model, known pathophysiology, and evidence-based research composed the framework used in developing the diagnosis and management plan for an electronic record system in a free health care clinic.;The project was evaluated based on the following four objectives: (1) To provide an educational session at a …

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Molecular Characterization Of The Murf Gene Family: Potential Role In Rainbow Trout Muscle Degradation, Jiannan Wang May 2010

Molecular Characterization Of The Murf Gene Family: Potential Role In Rainbow Trout Muscle Degradation, Jiannan Wang

Graduate Theses, Dissertations, and Problem Reports (ETD)

As United States and world seafood demand increases, aquaculture production becomes more important. Limited knowledge of molecular regulation of muscle growth and flesh quality hinders genetic improvement of these important traits in fish. Our goal is to enhance muscle growth and fillet quantity in rainbow trout, Oncorhynchus mykiss. Muscle growth is determined primarily by rate of protein turnover. Unlike mammals, rapidly growing fish have reduced protein degradation rather than increased protein synthesis. Studies in mammals showed that muscle atrophy results from increased protein breakdown, and is associated with activation of the ubiquitin proteasome pathway including induction of the muscle-specific ubiquitin …

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A Proposed Method For Evaluation Of Morphological Changes In The Condyle And Glenoid Fossa By Cone Beam Computed Tomography, Michael R. Munn May 2010

A Proposed Method For Evaluation Of Morphological Changes In The Condyle And Glenoid Fossa By Cone Beam Computed Tomography, Michael R. Munn

Graduate Theses, Dissertations, and Problem Reports (ETD)

The difficulty with three-dimensional analyses remains with the myriad of data that is possible to derive from a volume. The goal of this study is to report 3D changes in the temporomandibular joint in a reliable and quantifiable way. The approach included plotting specific referents on the mandibular condyle and tracking them in magnitude (mm) and direction (°) on a reference plane after superimposing the cone beams three-dimensionally on the inferior alveolar nerve canal and the lower contour of the third molar tooth germ. Two sets of measurements were compared for reliability and each measurement showed varied correlation. Linear measurements …

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Satisfaction Of Contract Poultry Producers In Virginia And West Virginia, Christina L. Richmond May 2010

Satisfaction Of Contract Poultry Producers In Virginia And West Virginia, Christina L. Richmond

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study investigated the satisfaction of contract poultry producers in West Virginia and Virginia using a mailed questionnaire. Producers were asked to provide feedback on their company, income, debt load and grower relations. Producers indicated they were satisfied with their business decision to raise contract poultry. Level of satisfaction was significantly different for producers in the two states. Producers who raise pullets or broiler breeders exhibited greater satisfaction that broiler producers. The income level of producers has increased, however more producers have indicated they are losing money. Producers showed high levels of satisfaction with their service personnel who come directly …

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Aging-Dependent Effects Of Repetitive Loading Exercise And Antioxidant Supplementation On Oxidative Stress In Skeletal Muscle, Michael J. Ryan May 2010

Aging-Dependent Effects Of Repetitive Loading Exercise And Antioxidant Supplementation On Oxidative Stress In Skeletal Muscle, Michael J. Ryan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Aging is associated with a reduced ability to buffer oxidants along with an increase in oxidant production, resulting in chronic oxidative stress. Oxidative stress is a putative factor responsible for reducing function ability of skeletal muscle and increasing oxidative damage. The objective of this set of investigations was to evaluate the efficacy of reducing oxidative stress on improving muscle function the adaptive response of skeletal muscle to repetitive loading exercise in aging rodents. To achieve this objective, three methods of reducing oxidative stress were utilized; the antioxidant vitamins E&C were used to buffer oxidants, the nutraceutical resveratrol was used to …

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Stream Fish Metacommunity And Metapopulation Ecology In A Mined Appalachian Watershed, Roy Worth Martin May 2010

Stream Fish Metacommunity And Metapopulation Ecology In A Mined Appalachian Watershed, Roy Worth Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chapter one is a direct empirical evaluation of metacommunity theory using stream fishes in a heavily mined and least impaired region of the Monongahela River basin. Metacommunity ecology, a new multi-scale paradigm, has provided important insights into mechanisms controlling regional patterns of assemblage structure. Metacommunity ecology offers four competing hypothetical models---neutral, patch dynamic, species sorting, and mass effect---each differing slightly in its underlying mechanistic framework.;Our results overwhelmingly supported the mass effect metacommunity model, which suggests that regional patterns of diversity are maintained by local habitat heterogeneity and dispersal among local assemblages. However, our findings also indicated that natural communities are …

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Pretreatments And Energy Potentials Of Appalachian Hardwood Residues For Biofuel Production, Adebola Bamikole Adebayo May 2010

Pretreatments And Energy Potentials Of Appalachian Hardwood Residues For Biofuel Production, Adebola Bamikole Adebayo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Continuous increase in crude oil price and environmental issues related to toxic emissions from the use of petroleum products has necessitated the need to find an alternative renewable source of energy in woody biomass. Accordingly, properties, chemical pretreatments, and enzymatic hydrolysis of logging residues to fermentable sugars were evaluated in this study. Logging residue specimens of yellow-poplar (Liriodendron tulipifera) and red oak (Quercus rubra) were collected from fifteen previously harvested sites across West Virginia and then analyzed for their physical, chemical, and thermal properties. Results indicated that logging residues of yellow-poplar and red oak were dried in nature to a …

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Effects Of Prenatal Stress On Lever-Press Acquisition, Delay Discounting, And Ethanol Self -Administration In Rats, Natalie R. Bruner May 2010

Effects Of Prenatal Stress On Lever-Press Acquisition, Delay Discounting, And Ethanol Self -Administration In Rats, Natalie R. Bruner

Graduate Theses, Dissertations, and Problem Reports (ETD)

It is well established in animal research that the hypothalamic-pituitary-adrenal (HPA) axis plays a central mediating role in the detrimental effects of prenatal stress in mother and offspring. The prenatally stressed (PS) rat exhibits a number of behaviors indicative of increased HPA activity relative to control (CON) rats. Because of these behaviors, PS rats may learn novel responses more quickly than CON rats. However, there are mixed findings in the literature as to whether prenatal stress facilitates or impedes learning. Experiment 1 was designed to examine effects of prenatal stress on lever-press acquisition using 10-s delays to food pellet delivery …

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Alterations In Hepatic Enzyme Activity And Progesterone Clearance In Lactating Dairy Cows, Caleb Owens Lemley May 2010

Alterations In Hepatic Enzyme Activity And Progesterone Clearance In Lactating Dairy Cows, Caleb Owens Lemley

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the cow, inadequate concentrations of progesterone during gestation may lead to an abrupt termination of pregnancy. The primary organ involved in progesterone catabolism is the liver, which contains an abundance of cytochrome P450 isozymes (CYP; EC 1.14.14.1) and aldo-keto reductases (AKR; EC 1.1.1.145-151), which are involved in the first phase of steroid inactivation, before second phase conjugation and excretion of the steroid metabolite by UDP-glucuronosyltransferases (UGT; EC 2.4.1.17). The objectives of the current experiments were to: (1) determine the effect of feeding two isoenergetic and isonitrogenous diets formulated to cause divergent insulin secretion, on hepatic progesterone catabolic enzyme activity …

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The Use Of Microbial Community Fingerprinting As A Marker For Tracking The Source Of Water: Application To Pathogen And Groundwater Source Tracking, Mary Clement May 2010

The Use Of Microbial Community Fingerprinting As A Marker For Tracking The Source Of Water: Application To Pathogen And Groundwater Source Tracking, Mary Clement

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microbial community structure in groundwater is extremely complex and constitutes a fingerprint of water. Terminal restriction fragment length polymorphism (T-RFLP) analysis is a polymerase chain reaction (PCR)-based fingerprinting method that is commonly used for comparative microbial community analysis, and to track specific bacterial markers in complex environments.;The Objective of this research was to propose an innovative approach for tracking the origin of groundwater, microbial source tracking and origin of surface water, based on the specific structure of the microbial community using T-RFLP analysis, which can be used for microbial source tracking.;The first specific aim was to validate the use of …

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Comparing The Use Of Qpcr On Isolated Smallmouth Bass (Micropterus Dolomieu) And Rainbow Trout (Oncorhynchus Mykiss) Hepatocytes For Estrogen Screening, Emily Breana Chambers May 2010

Comparing The Use Of Qpcr On Isolated Smallmouth Bass (Micropterus Dolomieu) And Rainbow Trout (Oncorhynchus Mykiss) Hepatocytes For Estrogen Screening, Emily Breana Chambers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Exposure of fish to estrogenic endocrine disrupting chemicals (EEDCs) is believed to be a contributing factor of intersex (the presence of immature oocytes in the testes) and fish kills in the Potomac and Shenandoah Rivers. EEDCs are often seen in the effluent of wastewater treatment plants. A simple and common way to look at the estrogenicity of water samples is to deploy Passive Organic Chemical Integrative Samplers (POCIS) and extract the hydrophilic compounds from the filters with methanol. Samplers were deployed upstream and downstream of two wastewater treatment plants, one on the Conococheague River and one on the Monocacy River, …

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Religious And Environmental Values In Pcusa And Ucc Church Camp Programs, Ross S. Bash May 2010

Religious And Environmental Values In Pcusa And Ucc Church Camp Programs, Ross S. Bash

Graduate Theses, Dissertations, and Problem Reports (ETD)

The association between denominational affiliation, as the independent variable, and (1) religious values, (2) environmental values, and (3) the integration of religious values and environmental values, as the dependent variables, in church camp programs of the Presbyterian Church (U.S.A.) (PCUSA) and the United Church of Christ (UCC) was examined through survey research of PCUSA and UCC church camps across the United States. The results indicated that there is a significant statistical relationship between PCUSA and UCC church camp programs by reference to denominational affiliation for all three dependent variables.

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Constraints To Participation In An Outdoor Orientation Program, Madeleine Hoden May 2010

Constraints To Participation In An Outdoor Orientation Program, Madeleine Hoden

Graduate Theses, Dissertations, and Problem Reports (ETD)

Outdoor orientation programs have shown success in helping students transition to college life. Research has shown that outcomes for students participating in a first year seminar include achieving higher grade point averages, higher persistence, measured by completion of a degree, and taking less time to complete a degree program. This study used Crawford, Godbey, and Jackson's hierarchical model of structural, interpersonal and intrapersonal constraints to leisure. An online survey was used to measure constraints to participation in Adventure WV. Students tended to perceive structural constraints as the most common constraints. Results indicated that female students tended to perceive intrapersonal constraints …

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Designing A Face Detection Captcha, Adam C. Day May 2010

Designing A Face Detection Captcha, Adam C. Day

Graduate Theses, Dissertations, and Problem Reports (ETD)

Completely Automated Tests for Telling Computers and Humans Apart (CAPTCHAs) are quickly becoming a standard for security in every online interface that could be the subject to spam or other exploitation. The majority of today's CAPTCHA technologies rely on text-based images, which present the user with a string of distorted characters and asks the user to type out the characters. The problem with CAPTCHAs is that they are often difficult to solve and can generally be successfully defeated using techniques such as segmentation and optical character recognition. We introduce an image face recognition based CAPTCHA which presents the user with …

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Residual Feed Intake, Body Composition And Fertility In Yearling Beef Heifers, Kevin S. Shaffer May 2010

Residual Feed Intake, Body Composition And Fertility In Yearling Beef Heifers, Kevin S. Shaffer

Graduate Theses, Dissertations, and Problem Reports (ETD)

One hundred thirty-seven spring born yearling beef heifers of English breed types were used to determine the relationships between residual feed intake (RFI) and growth rate, body composition, mature size, and fertility. Heifers were housed in a drylot facility during the trial and data were collected over a two-year period (year 1, n=67; year 2, n=70). Individual feed intake, body weight (BW), body condition score (BCS ), hip height (HH), and carcass ultrasound measurements (subcutaneous rib and rump fat; RIF and RUF, respectively, longissimus muscle area; LMA, and intramuscular fat; IMF ) were collected. Individual feed intakes were expressed as …

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Utilization Of Leaf Meal As A Potential Feed Source, Jason K. Smith May 2010

Utilization Of Leaf Meal As A Potential Feed Source, Jason K. Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Alternative feeds are currently utilized by producers throughout much of animal agriculture as a method of reducing feed costs. Under careful resource management, and proper understanding of their potential effects on animal performance, some deciduous tree leaves may prove to be a valuable feed resource, as substantial tonnage exists in the forests of the United States. In order to assess their value as a feed source, fall-dropped Quercus alba (white oak) and Liriodendron tulipifera (tulip poplar) leaves were collected at the Appalachian Farming Systems Research Center in Beaver, WV by the USDA-ARS. Proximate analysis and fiber determination, as well as …

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Bat Use Of Created And Natural Wetlands, Marcia L. Maslonek May 2010

Bat Use Of Created And Natural Wetlands, Marcia L. Maslonek

Graduate Theses, Dissertations, and Problem Reports (ETD)

Compensatory wetland mitigation is a common practice to account for wetland losses due to dredging and filling under the Clean Water Act, but successful replacement of function is rarely achieved. Small, isolated wetlands also receive no federal protection, and are usually not included in accounts of losses. Although latest reports show an increase in wetlands for the first time, this is due in large part to voluntary construction of open water ponds, while the loss of freshwater emergent wetlands continues to decline. Research on the wildlife functions of wetlands has focused on plants, invertebrates, avian or amphibian species. But wetlands …

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Topological Visualization Of Tensor Fields Using A Generalized Helmholtz Decomposition, Lierong Zhu May 2010

Topological Visualization Of Tensor Fields Using A Generalized Helmholtz Decomposition, Lierong Zhu

Graduate Theses, Dissertations, and Problem Reports (ETD)

Analysis and visualization of fluid flow datasets has become increasing important with the development of computer graphics. Even though many direct visualization methods have been applied in the tensor fields, those methods may result in much visual clutter. The Helmholtz decomposition has been widely used to analyze and visualize the vector fields, and it is also a useful application in the topological analysis of vector fields. However, there has been no previous work employing the Helmholtz decomposition of tensor fields. We present a method for computing the Helmholtz decomposition of tensor fields of arbitrary order and demonstrate its application. The …

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Long Term Ampk Activation Limits Obesity Induced Muscle Atrophy, Joshua C. Drake May 2010

Long Term Ampk Activation Limits Obesity Induced Muscle Atrophy, Joshua C. Drake

Graduate Theses, Dissertations, and Problem Reports (ETD)

The aim of this study was to identify obesity-induced alterations in regulatory mechanisms of skeletal muscle mass and how they would be altered with long term (8 weeks) AMPK-agonist treatment. 8 week old male, lean (L) wild type [body weight (BW) = 26.9 g] and ob/ob (O) [BW = 46.2 g] mice were fed an AMP kinase (AMPK) activator, beta-GPA (F), mixed at a 1% concentration within their food or normal chow as control (C) for 8 weeks. Following an overnight (12 hr) fast, all mice were sacrificed and the gastrocnemius complex was excised for analysis. Muscle mass was lower …

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Vibrant Amusement, Bretton J. Kern May 2010

Vibrant Amusement, Bretton J. Kern

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis is a description and analysis of the ceramic work of Bretton J. Kern. This thesis includes a description and images of the work as well as a discussion of the technical and conceptual content of the work.

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The Effects Of Non-Thermal Electron Beam Irradiation On Quality Indicators Of Peanut Butter, Amanda El-Rawas Jan 2010

The Effects Of Non-Thermal Electron Beam Irradiation On Quality Indicators Of Peanut Butter, Amanda El-Rawas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Electron beam (e-beam) irradiation is an effective non-thermal processing step for the reduction of Salmonella in peanut butter. The objective of this study was to evaluate the effects of e-beam irradiation on the quality of peanut butter. Peanut butter samples were exposed to e-beam doses of 0 (control), 3.2, 7.0, or 27.7 kGy, confirmed by film dosimetry. Doses correlated with a 5- and 12-log reduction of Salmonella respectively; with 27.7 kGy representing an extreme dose. Samples were examined over a 15-day period at 22°C. Color analysis (L, a*, b*), spreadability, and thiobarbituric acid reactive substances test (TBARS) for lipid oxidation …

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On The Mechanisms Underlying Neural Repression By E(Spl)M8 In Drosophila, Bhaskar Kahali Jan 2010

On The Mechanisms Underlying Neural Repression By E(Spl)M8 In Drosophila, Bhaskar Kahali

Graduate Theses, Dissertations, and Problem Reports (ETD)

Notch signaling is an evolutionary conserved pathway that mediates binary cell-fate specification throughout animal development. Through a process termed lateral inhibition, Notch signaling drives two equipotent cells to adopt distinct fates. Binary cell-fate determination has been exceptionally well studied during Drosophila neurogenesis, particularly, in eye and bristle development. In the eye, Notch mediates the selection of R8 photoreceptors from clusters of R8 precursors by antagonizing the activity of the proneural activator Atonal (Ato). In the bristle, Notch drives the selection of the sensory organ precursors (SOP's) from a group of equipotential cells that expressed the proneural activators encoded by the …

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Brook Trout Response To Canopy And Large Woody Debris Manipulations In Appalachian Streams, Jonathan Ma. Niles Jan 2010

Brook Trout Response To Canopy And Large Woody Debris Manipulations In Appalachian Streams, Jonathan Ma. Niles

Graduate Theses, Dissertations, and Problem Reports (ETD)

The forested riparian area along many central Appalachian streams contains large volumes of harvestable timber. Best management practices (BMP) and streamside management zones (SMZ) have been developed to minimize the impacts of riparian timber harvest. Large woody debris (LWD) is an important component of forested streams and its role in chemical, biological, and physical processes in streams is complex. The extraction of timber within the streamside management zone reduces the amount of material available for aquatic structure.;Three-250 m study reaches were established on eight Appalachian headwater streams. Four of the streams were assigned the treatment of having a 50% basal …

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Effects Of Essential Oils On Rumen Fermentation, Eating Behavior And Milk Production In Lactating Dairy Cattle, Lisa Rachel Tager Jan 2010

Effects Of Essential Oils On Rumen Fermentation, Eating Behavior And Milk Production In Lactating Dairy Cattle, Lisa Rachel Tager

Graduate Theses, Dissertations, and Problem Reports (ETD)

The objectives of the current studies were to: (1) investigate the effects of cinnamaldehyde (CIN), eugenol (EUG), and capsicum oil (CAP) on rumen fermentation and pH characteristics in continuous culture using a corn-based dairy ration; (2) to assess the in vivo effects of CAP at the recommended dosage, a blend of CIN and EUG at the recommended dosage, and a blend of CIN and EUG at a high dosage closer to levels used in vitro, on rumen fermentation, rumen pH, milk production, and feeding behavior; and (3) to assess the effects of CAP in a larger scale study on eating …

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Aztec Musical Styles In Carlos Chavez's "Xochipilli: An Imagined Aztec Music" And Lou Harrison's "The Song Of Quetzalcoatl": A Parallel And Comparative Study., Shawn M. Roberts Jan 2010

Aztec Musical Styles In Carlos Chavez's "Xochipilli: An Imagined Aztec Music" And Lou Harrison's "The Song Of Quetzalcoatl": A Parallel And Comparative Study., Shawn M. Roberts

Graduate Theses, Dissertations, and Problem Reports (ETD)

From the appearance of Amaldeo Roldan's Ritmica No. 5 and No. 6 in 1930 and Edgard Varese's Ionisation in 1931, until the United States involvement in World War II brought a hiatus to the genre, over fifty works for percussion ensemble were published. At the height of this outpouring, two works for percussion ensemble on a similar cultural topic were written, Carlos Chavez's Xochipilli: An Imagined Aztec Music (1940) and Lou Harrison's The Song of Quetzalcoatl (1941). Within Chavez and Harrison's titles are the names of two well-known and highly revered Aztec deities, Xochipilli and Quetzalcoatl. Consequently, this project determines …

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Extracellular Matrix Mediated Cartilage Regeneration: A Novel Three-Dimensional In Vitro Microenvironment To Facilitate Cell Expansion And Chondrogenesis, Fan He Jan 2010

Extracellular Matrix Mediated Cartilage Regeneration: A Novel Three-Dimensional In Vitro Microenvironment To Facilitate Cell Expansion And Chondrogenesis, Fan He

Graduate Theses, Dissertations, and Problem Reports (ETD)

Joint articular cartilage is vulnerable for degenerative disease after trauma due to the limited self-healing ability. Mesenchymal stem cells (MSCs) are considered a promising source for cartilage repair and regenerative medicine because of their excellent capacities of self-renewal and multipotent differentiation. Conventional in vitro expansion system, however, limits the utilization of MSCs for cartilage regeneration because in monolayer culture MSCs rapidly lose their self-renewal ability and differentiation potential. The lack of proper microenvironment in the current two-dimensional expansion system is attributed to the loss of self-renewal and multipotent differentiation in MSCs. Three-dimensional (3D) cell-deposited extracellular matrix (ECM) provides a novel …

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Development Of Nanofluidic/Microfluidic Interfaces As Analyte Concentrators For Proteomic Samples, Kathleen C. Reschke Jan 2010

Development Of Nanofluidic/Microfluidic Interfaces As Analyte Concentrators For Proteomic Samples, Kathleen C. Reschke

Graduate Theses, Dissertations, and Problem Reports (ETD)

We have developed a nanofluidic microfluidic analyte concentrator for application to proteomic samples. This dissertation includes a study investigating the current rectification behavior of the device including a temporal characterization of the ON state to OFF state transition and the OFF state to ON state transition as a function of device design. An analyte concentrator compatible with proteomic samples is presented and the current rectifying phenomenon is utilized to ensure adequate analyte delivery for the enrichment process. Finally, characterization of a coating technique to alter the surface charge of the nanofluidic element from anionic to cationic is presented.

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