Digital Commons Network^™

Exploring Teacher Perception And Implementation Of Sae Programming In West Virginia, Joseph Laflamme

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to describe the phenomenon of SAE implementation in West Virginia. This study utilized qualitative research methodology to describe teacher perceptions of how student, guardian, school and community factors affect SAE program implementation. Additionally, teacher backgrounds, including formative experiences, training and education, guiding philosophies, skills, and motivation were explored. This study describes contextual aspects of population, economics, state guidelines and local agriculture industries. This is done through a document review of student reported records as well as surveys and teacher interviews.

Back To The Future Self: Parsing Age Effects In Future Self-Continuity Through Goal Orientation And Life Satisfaction, Laken A. Mooney

Graduate Theses, Dissertations, and Problem Reports (ETD)

Future self-continuity, the psychological feeling of connectedness between the current and the future self, is a relatively novel concept that is associated with and has been used to predict a variety of outcomes. Though work investigating individual differences is comparatively rare, a robust demographic difference found in previous literature is the presence of an age effect, where older adults are more likely to feel a greater connection to their future self than younger adults. This finding has been replicated a number of times, but potential mechanisms behind this observed age-related increase have yet to be fully uncovered. Limited previous work …

Xanthine Oxidoreductase, Uric Acid, And Iron Homeostasis: Discovering A Therapeutic Vulnerability In Breast Cancer, Matthew G. Chapa

Graduate Theses, Dissertations, and Problem Reports (ETD)

Breast cancer remains the most frequently diagnosed malignancy and the leading cause of cancer related deaths in women. This is primarily due to distant metastases, which highlight the necessity to understand factors that contribute to breast cancer progression. Emerging evidence associates decreased tumor xanthine oxidoreductase (XOR) expression with a poorer prognosis, increased recurrence, and a more aggressive phenotype. While healthy liver, intestines, kidneys, and breast tissue have robust XOR expression and activity, there is a tendency for this to be ablated in carcinogenesis. This dissertation investigates XOR's role in breast cancer and identifies its product, uric acid (UA), as possessing …

Beyond The Byproduct: Exploring Approaches For The Sustainable Valorization Of Underutilized Food Streams, Ian Phillip Israelsen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Commercial food processing creates side-streams that are often underutilized yet hold significant potential for valorization into value-added products for consumer use. This study investigated and compared potential protein recovery and lipid removal potential from buckwheat (BW; Fagopyrum esculentum Moench), brewer’s spent grain (BSG), and coffee silverskin (CSS) using a one-step solvent defatting and a pH-shift protein extraction method. The extraction efficiency yields of extracted protein from CSS were significantly lower than all other samples (p < 0.05). The defatted BW had the highest extracted protein content of all samples (p < 0.05). The sizes of the proteins in the starting materials were observed using LDS-PAGE. These sizes were consistent with published values and were mostly unchanged by defatting or the pH-shift process. The presence of 13S globulins and 2S albumins was confirmed under reducing conditions. Protein extracted from all native samples had lower total free essential amino acids than the starting materials. Native CSS and extracted protein followed this trend, but in defatted CSS and respective extracted protein samples, the extracted proteins had higher free essential amino acid content than the starting materials, sometimes of twofold or greater (p < 0.05). After defatting, the percentage of lipids recovered compared to the original lipid content of the samples was measured. The crude fat content of native and hexane-defatted BW flour was higher than BW flour defatted with ethanol (p < 0.05). The lipid extraction efficiency of all samples ranged from 4.6 ± 1.26%–43.20 ± 14.21%. The mass of the native and defatted starting materials were recorded at each step of processing, with reasonable losses assessed. The major non-polar lipid classes in each starting material were separated using thin-layer chromatography. All samples also displayed the presence of polar lipids. When comparing starting materials, BW and BSG was the most effective for protein extraction, and BSG has the most promise for lipid removal and potential for upcycling. All the examined starting materials show promise for use in food supplementation, especially as native flours. These products are plentiful and considered underutilized or side-stream products.

Do Dung Beetles Sink Or Swim: Exploring Dung Beetle Response To Precipitation, Lauren T. Cheshire

Graduate Theses, Dissertations, and Problem Reports (ETD)

Global climate change constitutes a major threat to biodiversity and ecosystem services of many taxa. The Mid-Atlantic region of the US is expecting to see an increase in extreme precipitation events and flooding. Dung beetles (Scarabaeinae) can tunnel below the soil surface allowing for water to dissipate into the soil rather than sit on the surface and add to flooding conditions. They also can decrease methane emission by burying dung and reduce outbreaks of pests that prefer the moist environment of dung. They contribute roughly $380 million in ecosystem services annually in the United States, yet their services may be …

Effects Of High-Frequency Repetitive Transcranial Magnetic Stimulation On Impulse Control Disorders In Patients With Parkinson’S Disease On Dopamine Replacement Therapy, Katherine Muksuris

Graduate Theses, Dissertations, and Problem Reports (ETD)

Parkinson’s Disease (PD) is a debilitating neurodegenerative disorder that causes motor deficits, cognitive impairment, and depression. The mainstay treatment for motor symptoms is dopamine replacement therapy (DRT). DRT takes the form of levodopa or dopamine receptor agonists (DAs) and can be associated with impulse control disorders (ICDs). Currently, the approach to mitigating these side effects is to decrease or stop DRT, which may cause motor symptoms to return, making the management of the disorder more difficult. Repetitive transcranial magnetic stimulation (rTMS) is a noninvasive neuromodulation treatment for multiple disorders, including its approved use for smoking cessation. It has also shown …

Influence Of Different Rates Of Biochar And Deer Browsing On Pollinator Plant Productivity, Bipana Bhusal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Biochar, a carbon-rich material produced from biomass, has received wide attention for soil amendment to improve plant growth. However, its specific effects on pollinator friendly plants remain poorly understood. In this study, we evaluated the effects of four biochar application rates (0, 10, 20, 40 tons/acre) and deer exclusion (fenced vs. unfenced) on the productivity of three pollinator species; Buckwheat (Fagopyrum esculentum), Plains Coreopsis (Coreopsis tinctoria), and Black-eyed Susan (Rudbeckia hirta) in a utility right of way with a history of mining. A split-plot randomized design was used, and the results were analyzed using …

Apostrophes And Archimedes, Evan J. Clough

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis explores how digital collage and printmaking can be used to develop images through acts of subjective response, intentional and unintentional acts of appropriation, and parodic re-use and comparison pushed to absurd extremes. It looks at the history of creating art for parodic re-use and examines “Pataphysics”, developed by and for art appropriation of pattern, form, and process. These concepts are explained and related to visual artwork that was made for the thesis exhibition Apostrophes and Archimedes Cast Shadow Puppets. The exhibition invites viewers to experience the phenomenon of observation, automatic transformation, and creation using multiple modalities of exaggerated …

Computational Approaches For Diagnosing Neurological Impairments And Restoring Functional Movement, Serhii Bahdasariants

Graduate Theses, Dissertations, and Problem Reports (ETD)

Movement is the primary means by which the nervous system converts intention into interaction with the environment. Voluntary limb motion enables standing, walking, reaching, and grasping—actions that underpin self‑care, communication, and economic participation. When diseases or injuries disrupt the pathways that couple neural commands to muscle forces—whether through cortical stroke, spinal cord injury, or peripheral nerve damage—mechanical outcomes such as weakness, loss of coordination, and ultimately reduced mobility emerge. These impairments cascade into secondary complications, increased dependence on caregivers, and diminished quality of life. Quantifying how the lesion alters the mechanics of movement is therefore essential for designing targeted interventions …

Soft Epilogue, Chloe C. Firth

Graduate Theses, Dissertations, and Problem Reports (ETD)

This written document is the accompanying thesis for my Master of Fine Arts Exhibition, Soft Epilogue. The work in the exhibition serves as a visual record of my eating disorder recovery process. Each piece in the exhibition addresses a selected writing prompt from the book 8 Keys to Recovery from an Eating Disorder, published in 2011 and written by therapists and former eating disorder patients Carolyn Costin and Gwen Schubert Grabb. The book provides information and journal assignments regarding the development of eating disorders, how to maintain the motivation to recover, and how to navigate the complicated emotions …

Efficacy Of Salmonella Surrogate Enterococcus Faecium In Poultry And Mash Broiler Feed, Corey Waitman Coe

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation investigates the efficacy of Enterococcus faecium as a surrogate for Salmonella in validating thermal inactivation processes during poultry feed manufacturing and meat processing. The research encompassed three studies evaluating microbial kinetics in mash broiler feed, reconstructed ground chicken, and predictive modeling approaches to improve food safety interventions. In the first study, the thermal resistance of nalidixic acid-resistant Salmonella Typhimurium and E. faecium was compared in mash broiler feed heated at 75–95°C. Results demonstrated that E. faecium exhibited significantly greater heat resistance than Salmonella, with longer shoulder times, greater tailing effects, and higher D-values, supporting its use as …

Customer Segmentation And Fuel Economy Prediction Using Telemetry Data From Heavy-Duty Trucks, Batishahe Selimi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Heavy-duty trucks constitute only a modest fraction of on-road vehicles, yet their intensive duty cycles and high fuel demands yield a disproportionately large share of transportation fuel use and greenhouse gas emissions. Addressing this imbalance requires data-driven tools that capture the realities of fleet operation and translate complex telemetry into actionable insight.

This dissertation introduces a unified machine-learning framework that operates exclusively on high resolution time-series data collected from fifty-nine diesel trucks deployed across Southern California. It begins by constructing a multi-modal feature space that blends statistical summaries of key engine signals, static vehicle descriptors, and Mel-Frequency Cepstral Coefficients, thereby …

Neuroendocrine Regulation Of The Luteinizing Hormone Surge And Puberty Onset In Sheep, Max J. Griesgraber

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), and follicle stimulating hormone (FSH) are essential for the reproduction of all mammalian species, including both sheep and humans. GnRH is secreted by hypothalamic neurons into the hypophyseal portal vasculature in a pulsatile manner, which then acts within the anterior pituitary to elicit the release of LH and FSH into wider systemic circulation. In females LH and FSH act on the ovaries to promote estradiol (E2) production, follicular development, and under certain conditions in regard to LH, ovulation. Furthermore, while E2 inhibits GnRH and subsequent LH secretion under most conditions, at high concentrations E2 …

Molecular Characterization Of Fungus-Arthropod Associations, Angie M. Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Arthropods, or phylum Arthropoda, are the largest group of animals, accounting for over three quarters of all animal records on NCBI Taxonomy. And yet, only 40% of all deposited sequences on NCBI Nucleotide come from arthropods. Clearly, arthropods are an understudied group of animals: they are small, often drab, and lead lives very alien to our own. Arthropods have chitinous exoskeletons, segmented bodies, and jointed appendages, and include crabs, barnacles, insects, spiders, and millipedes. Since the era of DNA based studies took off in the 1980s, DNA and other small molecules have been integral to our understanding of the Earth …

Comparative Analysis Of Electrode Encapsulation Reliability: Planar Vs. Thermoformed Interdigitated Neural Electrodes In Biomedical Engineering, Tatum Peyerl

Graduate Theses, Dissertations, and Problem Reports (ETD)

Durable dielectric encapsulation is crucial for the long-term function of implantable neural electrodes; however, the reliability of curved, thermoformed coatings remains underexplored. This study addresses that gap by comparing planar (non-thermoformed) and thermoformed interdigitated neural electrodes (IDEs) encapsulated in Polyimide-2611. Both device types underwent accelerated in vitro aging in phosphate‑buffered saline (PBS) at 67 °C for approximately 91 days, simulating two years of physiological exposure. Post-accelerated aging evaluation included electrochemical impedance spectroscopy (EIS) from 0.1 Hz to 100 kHz to monitor moisture‑induced leakage, and high‑voltage breakdown testing under saline immersion to assess dielectric strength. After aging, all electrodes maintained impedance …

Evaluating Osmia Cornifrons As An Effective Pollinator For Agricultural Crops: An Analysis Of Hair Morphology And Sex-Ratio Distorting Bacterium, Wolbachia, Nellie Heitzman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Effective pollination relies not only on the presence of pollinators but also on their morphological adaptations and reproductive biology. In this study, I investigated both the external hair morphology and the intracellular bacterial infection status of Osmia cornifrons, a solitary bee species increasingly used in orchard pollination due to its efficiency and early seasonal activity. Using Scanning Electron Microscopy (SEM), I characterized the distribution and structure of two major hair types present on the bee's body: branched and unbranched. Branched hairs were found widely distributed across the head, thorax, dorsal abdomen, and legs, and appeared to function primarily in incidental …

P23h Rhodopsin Accumulation Disrupts Synaptic Protein Levels In Rod Photoreceptors, Samantha Thompson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rod photoreceptors are specialized neurons located in the retina, the neural tissue that lines the back of the eye. Rod photoreceptors are necessary for dim light and peripheral vision through the photoactivation rhodopsin (Rho). Rho is synthesized in the inner segment of rods before being unidirectionally trafficked to the outer segment. In diseases such as retinitis pigmentosa (RP), Rho trafficking can be disrupted, leading to progressive rod cell death. In many cases of RP, Rho is mislocalized to the rod photoreceptor synapse (“spherule”). The impact of Rho mislocalization on spherule structure and protein organization is poorly understood. We hypothesize that …

Media Framing Of The Local And National News Coverage Of The East Palestine Train Derailment, Lauren Delo

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research examined digital news coverage of the East Palestine train derailment to determine the frames and dominant frames used in both local and national coverage of the disaster, and whether these frames differed over time. Specifically, this research used a content analysis of digital newspaper articles from five media outlets—three national level and two local level—and applied the frames identified from Colistra’s (2010) study of print news coverage of the 1972 Buffalo Creek mine disaster. Results revealed significant differences between the most frequently used frames between local and national coverage, shifts in frame usage over time within each media …

Investigating The Physiological, Metabolomic, And Transcriptomic Changes In Response To Telomerase Deletion In Saccharomyces Cerevisiae, Taizina Momtareen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Telomerase is an enzyme found in rapidly dividing cells that maintains chromosome ends and prevents the loss of genetic information during cell division. When telomerase is inactive, cells gradually lose their telomeres and eventually stop dividing. In yeast, a rare subset of cells can bypass this growth arrest by switching to a recombination-based mechanism to maintain telomeres. Therefore, yeast provides a powerful system to study this adaptation, as telomerase-deficient strains can generate 'survivors' that mimic the Alternative Lengthening of Telomeres (ALT) pathway observed in some human tumors. In this work, my goal was to examine how a telomerase-null (TLC1 …

Exploration Of Coping And Mental Health In West Virginia Farmers, Jodi Meadows Richmond

Graduate Theses, Dissertations, and Problem Reports (ETD)

Compared nationally, West Virginia currently ranks lowest for life expectancy, highest for drug related deaths and 35^th for access to adequate health (WV DHHR Organization Assessment & Strategic Plan, 2022). The state also ranks first in the nation for depression with one in four adults experiencing some form of depressive condition each year (CDC, 2020). The rurality of the state may limit social interaction and availability of medical treatment facilities (Brew et al., 2016).

The situation is even more dire for those in farming-related occupations. Farmers often struggle with management stress, utilizing appropriate coping strategies, and accessing mental health …

White Paper And Black Water: Environmental Control Of Labor In Appalachian Paper Mill Communities, Matthew C. Powell

Graduate Theses, Dissertations, and Problem Reports (ETD)

In several small Appalachian communities, the West Virginia Pulp & Paper Company dominated the local landscape. The company stripped the mountainsides of spruce trees for pulpwood and used rivers for industrial processes and waste disposal. The small towns along these rivers and between these mountains relied on paper mills for employment. Corporate control over the natural environment, and water more specifically, was a necessary requirement for paper production, but also acted as a source of power over employees and their communities. When paper workers sought to organize, the company responded by leveraging their control over the environment against them to …

From Sequencing To Conservation: Genomic Analysis Of Three Sportfish Species In West Virginia, Andrew Johnson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Situated in the Appalachian Mountains, one of the oldest mountain ranges on Earth, West Virginia waters boast rich ichthyofauna including native sportfish species walleye (Sander vitreus), largemouth bass (Micropterus nigricans), and muskellunge (Esox masquinongy). These three species are all native to the contemporary Ohio River watershed and play a major recreational and ecological role in local fisheries. Ecologically, all three species are apex predators and play a key role in ecosystems by directly influencing local fish assemblages. As dominant apex predators all three species are highly sought after in recreational fisheries with largemouth bass …

Evidence-Based Lighting Design For Healthcare: Health Effects Of Timed Blue-Light At Night (Blan) On Night-Shift Staff, Seyedeh Nazli Hosseini

Graduate Theses, Dissertations, and Problem Reports (ETD)

The effects of light at night on night-shift clinical staff in healthcare settings is a topic of growing significance, considering the widespread adoption of artificial lighting and the increasing prevalence of night-shift work in the healthcare industry. The intersection of neuroscience and architecture provides a unique lens to explore the intricate relationship between lighting conditions and the circadian hygiene of night-shift medical staff. This project investigates the strategies associated with light exposure schedules for staff during night-shift work in healthcare settings.

Exposure to artificial light at night may contribute to mood disturbances and sleep disorders and consequently affect alertness and …

Simulation Of Annpet For Neural Network Training And System Performance Benchmarking, Pete Martone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Monte Carlo (MC) simulations are often used to provide insight into complex physical phenomenon. They can generate realistic results without the necessity to construct costly and complex apparatus. The ultimate value of these simulations depend on how accurately the physics can be modeled and if a sufficient volume can be produced to account for statistical variance. In medical imaging device development, MC simulations of Positron Emission Tomography (PET) scanners have typically been used to model the performance of new systems. The imaging group in the Department of Radiology have developed a unique detector design for a pre- clinical PET scanner …

Communicated Narrative Sensemaking In The Grandparent-Grandchild Relationship, Madison Clara Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Storytelling is a primary form of communication between grandparents and grandchildren that has been shown to strengthen relational bonds and enhance grandchildren’s well-being. However, the specific narrative features and interactional processes involved in grandparent-grandchild storytelling remain underexplored, particularly regarding how they influence well-being and perceptions of generational differences. Guided by Communicated Narrative Sensemaking (CNSM) theory, this study examined the content (story framing) and process (interactional sensemaking behaviors) of grandparent-grandchild storytelling episodes as reported by young adult grandchildren (N = 110). Participants completed an online survey in which they recounted a storytelling episode with a grandparent and reported on individual well-being …

Filling Research Gaps In Occupational Health In Thermal Spray Coatings: A Scoping Review, Lab Characterization, And Lung Deposition Of Cold Sprayed Emissions, Bailley N. Reggetz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Thermal Spray Coating (TSC) processes are industrial surface treatment methods used to create coatings for repair, protection, and performance enhancement of components exposed to wear, corrosion, heat, or other harsh conditions. The most commonly used TSC techniques (flame spraying, electric arc spraying, HVOF spraying, plasma spraying, and cold spraying) are used across numerous industries including aerospace, automotive, biomedicine, and energy due to their ability to enhance surface properties such as providing corrosion resistance, wear resistance, and thermal insulation. While TSC techniques have been commercially available for nearly a century, they have undergone continuous development to accommodate more specialized applications, unique …

Revitalizing The Urban Riverfront: Redesigning The West End Bridge In Pittsburgh,Pa For Sustainable Multi-Use Connectivity And Public Space Creation., Mina Kargari Rezapour

Graduate Theses, Dissertations, and Problem Reports (ETD)

Urban waterfronts are critical assets for cities, offering opportunities for ecological restoration, public engagement, and multi-modal transportation. However, many riverfronts remain underutilized due to infrastructure barriers and lack of accessibility.

Pittsburgh’s riverfront is dynamic asset that supports recreation, cultural expression, and community interaction. The Three River Heritage Trail Loop, a visionary project led by River Life over the past 20 years, seeks to connect diverse neighborhoods and natural assets along the city’s rivers. At the heart of this initiative is the West End Bridge, serving as the bookend and a pivotal gateway to complete the loop and critical link for …

Evaluation And Identification Of Novel Viruses In Wild Fishes Using Varied Molecular Biology Techniques, Clayton Douglas Raines

Graduate Theses, Dissertations, and Problem Reports (ETD)

Viruses are among the most abundant biological entities on Earth, yet their diversity and ecological roles in aquatic vertebrates, particularly fishes, remain poorly characterized. This dissertation advances fish virology through integrative approaches that combine historical context, molecular diagnostics, and genomic discovery. A novel hepadnavirus (ApHBV) was identified in asymptomatic Alosa pseudoharengus, emphasizing the prevalence of subclinical infections in migratory forage species. In Catostomus commersonii, transcriptomic profiling revealed sexually dimorphic immune responses to hepatitis B and C-like viruses, highlighting complex host-pathogen dynamics. In Micropterus nigricans, next-generation sequencing uncovered a novel nackednavirus and multiple adomaviruses within hyperpigmented lesions, expanding …

Neurobiological Variables Mediating The Relationship Between Breast Cancer And Sleep Disruption, Brittany Domenique Elliott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neurobiological Variables Mediating the Relationship between

Breast Cancer and Sleep Disruption

Brittany D. Elliott, MS

Breast cancer is the most commonly diagnosed malignancy in women, with more than two million new cases diagnosed world-wide each year. Improvements in screening technology and diagnostics have increased survival rates, but cancer-associated sleep disruptions persist for the majority of breast cancer survivors well beyond the treatment period. In addition to reducing quality of life, reduced sleep quality is associated with greater incidences of affective dysfunction, reduced treatment efficiency, aggressive cancer progression, increased symptom severity, and increased mortality by all causes. Thus, identifying the variables …

Non-Lethal Sampling Methods For Fish Health Assessments Using Smallmouth Bass Micropterus Dolomieu, Lindsey Nicole Hartzell

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the early 2000s, fish kills of different species, including smallmouth bass Micropterus dolomieu, were reported in areas of the Potomac River drainage, including the Shenandoah River in Virginia. Since the first reports of fish kills and lesions affecting smallmouth bass, they have continued to show signs of adverse health effects. Additionally, in some areas of the Potomac River drainage, population declines have occurred, and lethal sampling is discouraged. To develop a holistic, integrative approach to assess fish health, Smallmouth Bass were collected and sampled using non-lethal techniques at three sites within the Shenandoah River drainage, VA and one …