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A Robust Experimental Design For Conceptual Model Discrimination Based On Information Theory, Hai V. Pham, Frank T.C. Tsai Aug 2023

A Robust Experimental Design For Conceptual Model Discrimination Based On Information Theory, Hai V. Pham, Frank T.C. Tsai

Faculty Publications

This study introduces firm information gain for model discrimination based on Shannon entropy and worst-case scenario experimental design. Firm information gain is the minimal additional information gained by an experimental design with respect to existing information. Robust experimental design aims to maximize the firm information gain by searching for the least number of new pumping wells and observation wells. Robust experimental design includes a Bayes factor threshold to ensure that new data provide strong evidence for model discrimination. To maximize the firm information gain, a framework is proposed that combines the parallel-sequential genetic algorithm (GA) for parallel computing and the …


Performance Optimization Study Of The Neuromorphic Radiation Anomaly Detector, James Ghawaly, Aaron Young, Andrew Nicholson, Brett Witherspoon, Nick Prins, Mathew Swinney, Cihangir Celik, Catherine Schuman, Karan Patel Aug 2023

Performance Optimization Study Of The Neuromorphic Radiation Anomaly Detector, James Ghawaly, Aaron Young, Andrew Nicholson, Brett Witherspoon, Nick Prins, Mathew Swinney, Cihangir Celik, Catherine Schuman, Karan Patel

Faculty Publications

This work reports on new results and insights from the optimization of spiking neural networks developed for gamma-ray radiation anomaly detection. Our previous paper introduced the first known neuromorphic algorithm for this application, demonstrating promising results and insights into optimal hyperparameter selection - particularly in the choice of data input encodings. Since the first paper, we have tested the algorithms on new datasets to investigate transferability from one background radiation environment to another. We have also performed a new hyperparameter optimization experiment with this new dataset to investigate the impact of new radiation data formatting techniques, the inclusion or neuronal …


The Water Mills Of The Historic Río Arriba In Northcentral New Mexico, 1598-1975, José A. Rivera Ph.D, Thomas F. Glick Ph.D Aug 2023

The Water Mills Of The Historic Río Arriba In Northcentral New Mexico, 1598-1975, José A. Rivera Ph.D, Thomas F. Glick Ph.D

Faculty Publications

The water mills of New Mexico played a major role in the agricultural economy of the Río Arriba for centuries following the introduction of wheat from the Old World to the Americas. Wheat, in its ground form as flour, was a staple during the Spanish colonial period. To process raw wheat, local grist mills (molinos) were essential infrastructure as were the aceq uias (ditches) that powered them. Situated near the banks of rivers, the internal components of each mill were driven by the gravity force of water from an acequia, itself diverted from the river. Researchers have documented …


Spectral Broadening Effects On Pulsed-Source Digital Holography, Steven A. Owens, Mark F. Spencer, Glen P. Perram Aug 2023

Spectral Broadening Effects On Pulsed-Source Digital Holography, Steven A. Owens, Mark F. Spencer, Glen P. Perram

Faculty Publications

Using a pulsed configuration, a digital-holographic system is setup in the off-axis image plane recording geometry, and spectral broadening via pseudo-random bit sequence is used to degrade the temporal coherence of the master-oscillator laser. The associated effects on the signal-to-noise ratio are then measured in terms of the ambiguity and coherence efficiencies. It is found that the ambiguity efficiency, which is a function of signal-reference pulse overlap, is not affected by the effects of spectral broadening. The coherence efficiency, on the other hand, is affected. As a result, the coherence efficiency, which is a function of effective fringe visibility, is …


Oxidation Of Hafnium Diboride—Silicon Carbide At 1500 °C In Air; Effect Of Compressive Stress, Anthony J. Degregoria, Marina B. Ruggles-Wrenn, Glen E. Pry Aug 2023

Oxidation Of Hafnium Diboride—Silicon Carbide At 1500 °C In Air; Effect Of Compressive Stress, Anthony J. Degregoria, Marina B. Ruggles-Wrenn, Glen E. Pry

Faculty Publications

The long-term oxidation behavior of HfB2 and of HfB2-20 vol.% SiC was studied. Test samples of each material were oxidized at 1500 °C in air using a box furnace. The exposure times were 0, 0.5, 1, 2, 3, 6, 9, 12, 15, 30, 45 and 90 h. Weight gain, oxide scale composition and oxide scale thickness were characterized for both materials. Crystal structure of the surface scales was analyzed using x-ray diffraction. Oxide scales were further characterized via scanning electron microscopy with energy dispersive spectroscopy analysis. For HfB2 the oxide scale consists predominantly of porous HfO …


Complexity Heliophysics: A [New] System Science That Transcends The Previous Boundaries Of Our Field, Ryan M. Mcgranaghan, Seebany Datta-Barua, Jeffrey Thayer, Joe Borovsky, Jay R. Johnson, Simon Wing, Dan Baker, Massimo Materassi Jul 2023

Complexity Heliophysics: A [New] System Science That Transcends The Previous Boundaries Of Our Field, Ryan M. Mcgranaghan, Seebany Datta-Barua, Jeffrey Thayer, Joe Borovsky, Jay R. Johnson, Simon Wing, Dan Baker, Massimo Materassi

Faculty Publications

Synopsis

1. The 21st century is the time of complexity. We delineate it and its importance as necessary to solve ‘wicked problems.’

2. Inherently transdisciplinary, trans-scale, and interconnected to living systems, the solution to Heliophysics’ identity crisis and to unlock the next generation of scientific discovery may be to embrace complexity.

3. With the right foresight, direction, and incentive over the next ten years, Heliophysics can become a beacon for how all of society thinks about and does complexity science.


Structural Behavior Of Reactive Powder Concrete Under Harmonic Loading, Shatha D. Mohammed, Teghreed H. Ibrahim, Ban F. Salman, Abbas A. Allawi Jul 2023

Structural Behavior Of Reactive Powder Concrete Under Harmonic Loading, Shatha D. Mohammed, Teghreed H. Ibrahim, Ban F. Salman, Abbas A. Allawi

Faculty Publications

Industrial buildings usually are designed to sustain several types of load systems, such as dead, live, and dynamic loads (especially the harmonic load produced by rotary motors). In general, these buildings require high-strength structural elements to carry the applied loads. Moreover, Reactive Powder Concrete (RPC) has been used for this purpose because of its excellent mechanical strength and endurance. Therefore, this study provides an experimental analysis of the structural behaviors of reinforced RPC beams under harmonic loads. The experimental program consisted of testing six simply supported RPC beams with lengths of 1500 mm, widths of 150 mm, and thicknesses of …


Large-Scale Nonequilibrium Molecular Studies Of Thermal Hydrate Dissociation, Meisam Adibifard, Olufemi Olorode Jul 2023

Large-Scale Nonequilibrium Molecular Studies Of Thermal Hydrate Dissociation, Meisam Adibifard, Olufemi Olorode

Faculty Publications

The energy content of methane hydrate reservoirs (MHRs) is at least twice that of conventional fossil fuels. So, there is considerable interest in their commercial development by heating, among other dissociation mechanisms. However, a few researchers have highlighted the potentially uncontrollable release of methane from MHRs, which could occur because of global warming. Therefore, it is crucial to understand the kinetics of thermal hydrate dissociation to safely develop these resources and prevent the release of this greenhouse gas into the environment. Although there have been several molecular studies of thermal dissociation, most of these use small simulation domains that cannot …


The Emergence Of Convergence, Shana M. Sundstrom, David G. Angeler, Jessica G. Ernakovich, Jorge H. Garcia, Joseph A. Hamm, Orville Huntington, Craig R. Allen Jul 2023

The Emergence Of Convergence, Shana M. Sundstrom, David G. Angeler, Jessica G. Ernakovich, Jorge H. Garcia, Joseph A. Hamm, Orville Huntington, Craig R. Allen

Faculty Publications

Science is increasingly a collaborative pursuit. Although the modern scientific enterprise owes much to individuals working at the core of their field, humanity is increasingly confronted by highly complex problems that require the integration of a variety of disciplinary and methodological expertise. In 2016, the U.S. National Science Foundation launched an initiative prioritizing support for convergence research as a means of “solving vexing research problems, in particular, complex problems focusing on societal needs.” We discuss our understanding of the objectives of convergence research and describe in detail the conditions and processes likely to generate successful convergence research. We use our …


Modulating Water Distribution And The Intramicellar Assembly Of Sequence-Defined Ionic Peptoid Block Copolymers By The Ionic Monomer Position, Bailee N. Barrett, Chi Huan Tung, Guan Rong Huang, Istiak Hossain, Chang Woo Do, Vijay T. John, Wei Ren Chen, Donghui Zhang Jul 2023

Modulating Water Distribution And The Intramicellar Assembly Of Sequence-Defined Ionic Peptoid Block Copolymers By The Ionic Monomer Position, Bailee N. Barrett, Chi Huan Tung, Guan Rong Huang, Istiak Hossain, Chang Woo Do, Vijay T. John, Wei Ren Chen, Donghui Zhang

Faculty Publications

The intramicellar mass heterogeneity of a series of sequence-defined ionic peptoid block copolymers carrying a single charged monomer has been determined through contrast variation small-angle neutron scattering analysis. We observe that the internal micellar structure, namely, the number density radial distributions of invasive water and peptoid polymer, is significantly impacted by the location of the ionic monomer. By positioning the ionic monomer progressively closer to the hydrophilic/hydrophobic block junction, the micelles become less compact with increasing levels of chain folding and invasive water to accommodate electrostatic repulsion among the ionic monomers via solvation. This results in increasingly smaller micellar aggregates …


Liposomes For The Treatment Of Brain Cancer—A Review, Richu Raju, Waad H. Abuwatfa, William G. Pitt, Ghaleb A. Husseini Jul 2023

Liposomes For The Treatment Of Brain Cancer—A Review, Richu Raju, Waad H. Abuwatfa, William G. Pitt, Ghaleb A. Husseini

Faculty Publications

Due to their biocompatibility, non-toxicity, and surface-conjugation capabilities, liposomes are effective nanocarriers that can encapsulate chemotherapeutic drugs and facilitate targeted delivery across the blood–brain barrier (BBB). Additionally, strategies have been explored to synthesize liposomes that respond to internal and/or external stimuli to release their payload controllably. Although research into liposomes for brain cancer treatment is still in its infancy, these systems have great potential to fundamentally change the drug delivery landscape. This review paper attempts to consolidate relevant literature regarding the delivery to the brain using nanocarriers, particularly liposomes. The paper first briefly explains conventional treatment modalities for cancer, followed …


A Review On Membrane Fouling Prediction Using Artificial Neural Networks (Anns), Waad H. Abuwatfa, Nour Al Sawaftah, Naif Darwish, William G. Pitt, Ghaleb A. Husseini Jul 2023

A Review On Membrane Fouling Prediction Using Artificial Neural Networks (Anns), Waad H. Abuwatfa, Nour Al Sawaftah, Naif Darwish, William G. Pitt, Ghaleb A. Husseini

Faculty Publications

Membrane fouling is a major hurdle to effective pressure-driven membrane processes, such as microfiltration (MF), ultrafiltration (UF), nanofiltration (NF), and reverse osmosis (RO). Fouling refers to the accumulation of particles, organic and inorganic matter, and microbial cells on the membrane’s external and internal surface, which reduces the permeate flux and increases the needed transmembrane pressure. Various factors affect membrane fouling, including feed water quality, membrane characteristics, operating conditions, and cleaning protocols. Several models have been developed to predict membrane fouling in pressure-driven processes. These models can be divided into traditional empirical, mechanistic, and artificial intelligence (AI)-based models. Artificial neural networks …


Wave Optics Approach To Solar Cell Brdf Modeling With Experimental Results, Madilynn Compean, Todd V. Small, Milo W. Hyde Iv, Michael Marciniak Jul 2023

Wave Optics Approach To Solar Cell Brdf Modeling With Experimental Results, Madilynn Compean, Todd V. Small, Milo W. Hyde Iv, Michael Marciniak

Faculty Publications

Light curve analysis is often used to discern information about satellites in geosynchronous orbits. Solar panels, comprising a large part of the satellite’s body, contribute significantly to these light curves. Historically, theoretical bidirectional reflectance distribution functions (BRDFs) have failed to capture key features in the scattered light from solar panels. In recently published work, a new solar cell BRDF was developed by combining specular microfacet and “two-slit” diffraction terms to capture specular and periodic/array scattering, respectively. This BRDF was experimentally motivated and predicted many features of the solar cell scattered irradiance. However, the experiments that informed the BRDF were limited …


A Hybrid Metaheuristic And Computer Vision Approach To Closed-Loop Calibration Of Fused Deposition Modeling 3d Printers, Graig S. Ganitano, Shay V. Wallace, Benji Maruyama, Gilbert L. Peterson Jul 2023

A Hybrid Metaheuristic And Computer Vision Approach To Closed-Loop Calibration Of Fused Deposition Modeling 3d Printers, Graig S. Ganitano, Shay V. Wallace, Benji Maruyama, Gilbert L. Peterson

Faculty Publications

Fused deposition modeling (FDM) is one of the most popular additive manufacturing (AM) technologies for reasons including its low cost and versatility. However, like many AM technologies, the FDM process is sensitive to changes in the feedstock material. Utilizing a new feedstock requires a time-consuming trial-and-error process to identify optimal settings for a large number of process parameters. The experience required to efficiently calibrate a printer to a new feedstock acts as a barrier to entry. To enable greater accessibility to non-expert users, this paper presents the first system for autonomous calibration of low-cost FDM 3D printers that demonstrates optimizing …


Simulation Study Of Shape Memory Polymer Networks Formed By Free Radical Polymerization, Collin D. Wick, Andrew J. Peters, Guoqiang Li Jul 2023

Simulation Study Of Shape Memory Polymer Networks Formed By Free Radical Polymerization, Collin D. Wick, Andrew J. Peters, Guoqiang Li

Faculty Publications

Chemically crosslinked thermoset shape memory polymers (TSMPs) can combine the strength required for load-carrying structures and devices with stimuli-responsive properties that can allow them to perform crack-closing or other multifunctional properties. In particular, UV-curable shape memory polymers can be used for 3D printing, and to enhance the design process, a molecular dynamics (MD) modeling framework was developed to study them. The MD simulations were used to calculate thermophysical, mechanical, and shape memory properties for glycerolate dimethacrylate after UV-curing to form a polymer network. Semiquantitative agreement between simulation and experiment was achieved for the different properties compared. By examining various conditions …


Hyperspectral Point Cloud Projection For The Semantic Segmentation Of Multimodal Hyperspectral And Lidar Data With Point Convolution-Based Deep Fusion Neural Networks, Kevin T. Decker, Brett J. Borghetti Jul 2023

Hyperspectral Point Cloud Projection For The Semantic Segmentation Of Multimodal Hyperspectral And Lidar Data With Point Convolution-Based Deep Fusion Neural Networks, Kevin T. Decker, Brett J. Borghetti

Faculty Publications

The fusion of dissimilar data modalities in neural networks presents a significant challenge, particularly in the case of multimodal hyperspectral and lidar data. Hyperspectral data, typically represented as images with potentially hundreds of bands, provide a wealth of spectral information, while lidar data, commonly represented as point clouds with millions of unordered points in 3D space, offer structural information. The complementary nature of these data types presents a unique challenge due to their fundamentally different representations requiring distinct processing methods. In this work, we introduce an alternative hyperspectral data representation in the form of a hyperspectral point cloud (HSPC), which …


Complex Systems Methods Characterizing Nonlinear Processes In The Near-Earth Electromagnetic Environment: Recent Advances And Open Challenges, Georgios Balasis, Michael A. Balikhin, Sandra C. Chapman, Giuseppe Consolini, Ioannis A. Daglis, Reik V. Donner, Jürgen Kurths, Milan Paluš, Jakob Runge, Bruce T. Tsurutani, Dimitris Vassiliadis, Simon Wing, Jesper W. Gjerloev, Jay Johnson, Massimo Materassi, Tommaso Alberti, Constantinos Papadimitriou, Pouya Manshour, Adamantia Zoe Boutsi, Mirko Stumpo Jul 2023

Complex Systems Methods Characterizing Nonlinear Processes In The Near-Earth Electromagnetic Environment: Recent Advances And Open Challenges, Georgios Balasis, Michael A. Balikhin, Sandra C. Chapman, Giuseppe Consolini, Ioannis A. Daglis, Reik V. Donner, Jürgen Kurths, Milan Paluš, Jakob Runge, Bruce T. Tsurutani, Dimitris Vassiliadis, Simon Wing, Jesper W. Gjerloev, Jay Johnson, Massimo Materassi, Tommaso Alberti, Constantinos Papadimitriou, Pouya Manshour, Adamantia Zoe Boutsi, Mirko Stumpo

Faculty Publications

Learning from successful applications of methods originating in statistical mechanics, complex systems science, or information theory in one scientific field (e.g., atmospheric physics or climatology) can provide important insights or conceptual ideas for other areas (e.g., space sciences) or even stimulate new research questions and approaches. For instance, quantification and attribution of dynamical complexity in output time series of nonlinear dynamical systems is a key challenge across scientific disciplines. Especially in the field of space physics, an early and accurate detection of characteristic dissimilarity between normal and abnormal states (e.g., pre-storm activity vs. magnetic storms) has the potential to vastly …


The Characteristics Of Successful Military It Projects: A Cross-Country Empirical Study, Helene Berg, Jonathan D. Ritschel Jul 2023

The Characteristics Of Successful Military It Projects: A Cross-Country Empirical Study, Helene Berg, Jonathan D. Ritschel

Faculty Publications

In the armed forces, successful digitalization is crucial to ensure effective operations. Much of the existing literature on project factors during the planning and execution phases of public IT projects do not focus specifically on military sector projects. Therefore, the paper aims to provide empirical insights into the characteristics of successful military IT projects. Data from such projects in NATO countries and agencies were collected through interviews and project documents. The findings relating to the main variable of interest, “delivery of client benefit,” supported previous findings on IT project performance. Medium-sized projects performed better than small and large projects, and …


Gender (In)Equity In Global Mental Health Research: A Call To Action, The Women In Global Mental Health Research Group, Kelly Rose-Clarke Jul 2023

Gender (In)Equity In Global Mental Health Research: A Call To Action, The Women In Global Mental Health Research Group, Kelly Rose-Clarke

Faculty Publications

In this commentary, we build on work by Gurung and colleagues which highlighted gender inequity in the global mental health research workforce in Nepal (Gurung et al., 2021). We seek to increase awareness of the under-representation of women in global mental health research and its consequences, and we call for change. By women, we refer to all people who identify as women, including trans people. The commentary is informed by conversations with women who are global mental health researchers in the Global North and South at various stages of their careers.


Electrically-Evoked Referred Sensations Induce Embodiment Of Rubber Limb, Anthony Nguyen, Brooke Draggoo, Brooklyn Tobias, Payton Dubose, Katharine Polasek Jul 2023

Electrically-Evoked Referred Sensations Induce Embodiment Of Rubber Limb, Anthony Nguyen, Brooke Draggoo, Brooklyn Tobias, Payton Dubose, Katharine Polasek

Faculty Publications

Introduction: Electrical stimulation is increasingly relevant in a variety of medical treatments. In this study, the quality of referred sensations evoked using surface electrical stimulation was evaluated using the rubber hand and foot illusions.

Methods: The rubber hand and foot illusions were attempted under 4 conditions: (1) multi-location tapping; (2) one-location tapping; (3) electrical stimulation of sensation referred to the hand or foot; (4) asynchronous control. The strength of each illusion was quantified using a questionnaire and proprioceptive drift, where a stronger response suggested embodiment of the rubber limb.

Results: 45 able-bodied individuals and two individuals with amputations participated in …


A Systematic Literature Review Of Cleanroom Ventilation And Air Distribution Systems, Arup Bhattacharya, Mohammad Saleh Nikoopayan Tak, Shervin Shoai-Naini, Fred Betz, Ehsan Mousavi Jul 2023

A Systematic Literature Review Of Cleanroom Ventilation And Air Distribution Systems, Arup Bhattacharya, Mohammad Saleh Nikoopayan Tak, Shervin Shoai-Naini, Fred Betz, Ehsan Mousavi

Faculty Publications

Cleanroom ventilation systems are well-established; however, the advantages and limitations of current practices need to be examined and explained further. This study begins by looking over the history of cleanroom ventilation systems that creates the basis for understanding ventilation rate specifications, terminologies employed in ventilation effectiveness, and recognizing scientific studies that correlate ventilation effectiveness with air change rates. This systematic review includes a comprehensive summary that contains a set of historical data and evidence that may be used to specify ventilation requirements in cleanrooms. Scientific articles are classified in terms of laboratory experiments, simulations/numerical analysis, or hybrid. HVAC designers and …


Extended Sentinel Monitoring Of Helicoverpa Zea Resistance To Cry And Vip3aa Toxins In Bt Sweet Corn: Assessing Changes In Phenotypic And Allele Frequencies Of Resistance, Galen P. Dively, Tom P. Kuhar, Sally V. Taylor, Helene Doughty, Kristian Holmstrom, Daniel O. Gilrein, Brian A. Nault, Joseph Ingerson-Mahar, Anders Huseth, Dominic Reisig, Shelby Fleischer, David Owens, Kelley Tilmon, Francis Reay-Jones, Pat Porter, Jocelyn Smith, Julien Saguez, Jason Wells, Caitlin Congdon, Holly Byker, Bryan Jensen, Chris Difonzo, William D. Hutchison, Eric Burkness, Robert Wright, Michael Crossley, Heather Darby, Tom Bilbo, Nicholas Seiter, Christian Krupke, Craig Abel, Brad S. Coates, Bradley Mcmanus Jul 2023

Extended Sentinel Monitoring Of Helicoverpa Zea Resistance To Cry And Vip3aa Toxins In Bt Sweet Corn: Assessing Changes In Phenotypic And Allele Frequencies Of Resistance, Galen P. Dively, Tom P. Kuhar, Sally V. Taylor, Helene Doughty, Kristian Holmstrom, Daniel O. Gilrein, Brian A. Nault, Joseph Ingerson-Mahar, Anders Huseth, Dominic Reisig, Shelby Fleischer, David Owens, Kelley Tilmon, Francis Reay-Jones, Pat Porter, Jocelyn Smith, Julien Saguez, Jason Wells, Caitlin Congdon, Holly Byker, Bryan Jensen, Chris Difonzo, William D. Hutchison, Eric Burkness, Robert Wright, Michael Crossley, Heather Darby, Tom Bilbo, Nicholas Seiter, Christian Krupke, Craig Abel, Brad S. Coates, Bradley Mcmanus

Faculty Publications

Transgenic corn and cotton that produce Cry and Vip3Aa toxins derived from Bacillus thuringiensis (Bt) are widely planted in the United States to control lepidopteran pests. The sustainability of these Bt crops is threatened because the corn earworm/bollworm, Helicoverpa zea (Boddie), is evolving a resistance to these toxins. Using Bt sweet corn as a sentinel plant to monitor the evolution of resistance, collaborators established 146 trials in twenty-five states and five Canadian provinces during 2020–2022. The study evaluated overall changes in the phenotypic frequency of resistance (the ratio of larval densities in Bt ears relative to densities in non-Bt ears) …


Substantially Enhanced Landscape Carbon Sink Due To Reduced Terrestrial-Aquatic Carbon Transfer Through Soil Conservation In The Chinese Loess Plateau, Lishan Ran, Nufang Fang, Xuhui Wang, Shilong Piao, Chun Ngai Chan, Siliang Li, Yi Zeng, Zhihua Shi, Mingyang Tian, Yi Jun Xu, Junyu Qi, Boyi Liu Jul 2023

Substantially Enhanced Landscape Carbon Sink Due To Reduced Terrestrial-Aquatic Carbon Transfer Through Soil Conservation In The Chinese Loess Plateau, Lishan Ran, Nufang Fang, Xuhui Wang, Shilong Piao, Chun Ngai Chan, Siliang Li, Yi Zeng, Zhihua Shi, Mingyang Tian, Yi Jun Xu, Junyu Qi, Boyi Liu

Faculty Publications

Soil conservation is of global importance, as accelerated soil erosion by human activity is a primary threat to ecosystem viability. However, the significance and role of soil conservation in reshaping landscape carbon (C) accounting has not been comprehensively integrated in the terrestrial C sink. Here, we present the first integrated assessment of the modified terrestrial C sink and aquatic C transport due to soil conservation for the semiarid Chinese Loess Plateau (CLP), the world's most vulnerable region to soil erosion. We show a surprisingly low terrestrial-aquatic C transfer that offset the terrestrial net ecosystem productivity by only 7.5%, which we …


A Modified-Herringbone Micromixer For Assessing Zebrafish Sperm (Mags), Jorge A. Belgodere, Mustafa Alam, Valentino E. Browning, Jason Eades, Jack North, Julie A. Armand, Yue Liu, Terrence R. Tiersch, W. Todd Monroe Jul 2023

A Modified-Herringbone Micromixer For Assessing Zebrafish Sperm (Mags), Jorge A. Belgodere, Mustafa Alam, Valentino E. Browning, Jason Eades, Jack North, Julie A. Armand, Yue Liu, Terrence R. Tiersch, W. Todd Monroe

Faculty Publications

Sperm motility analysis of aquatic model species is important yet challenging due to the small sample volume, the necessity to activate with water, and the short duration of motility. To achieve standardization of sperm activation, microfluidic mixers have shown improved reproducibility over activation by hand, but challenges remain in optimizing and simplifying the use of these microdevices for greater adoption. The device described herein incorporates a novel micromixer geometry that aligns two sperm inlet streams with modified herringbone structures that split and recombine the sample at a 1:6 dilution with water to achieve rapid and consistent initiation of motility. The …


Gene Editing Provides A Tool To Investigate Genes Involved In Reproduction Of Pigs, Rodney D. Geisert, Destiny N. Johns, Caroline A. Pfeiffer, Riley M. Sullivan, Caroline G. Lucas, Constantine A. Simintiras, Bethany K. Redel, Kevin D. Wells, Thomas E. Spencer, Randall S. Prather Jul 2023

Gene Editing Provides A Tool To Investigate Genes Involved In Reproduction Of Pigs, Rodney D. Geisert, Destiny N. Johns, Caroline A. Pfeiffer, Riley M. Sullivan, Caroline G. Lucas, Constantine A. Simintiras, Bethany K. Redel, Kevin D. Wells, Thomas E. Spencer, Randall S. Prather

Faculty Publications

CRISPR-Cas9 gene editing technology provides a method to generate loss-of-function studies to investigate, in vivo, the specific role of specific genes in regulation of reproduction. With proper design and selection of guide RNAs (gRNA) designed to specifically target genes, CRISPR-Cas9 gene editing allows investigation of factors proposed to regulate biological pathways involved with establishment and maintenance of pregnancy. The advantages and disadvantages of using the current gene editing technology in a large farm species is discussed. CRISPR-Cas9 gene editing of porcine conceptuses has generated new perspectives for the regulation of endometrial function during the establishment of pregnancy. The delicate orchestration …


Economic Viability Of Implementing Structural Health Monitoring Systems On The Support Structures Of Bottom-Fixed Offshore Wind, Mario Vieira, Brian Snyder, Elsa Henriques, Craig White, Luis Reis Jul 2023

Economic Viability Of Implementing Structural Health Monitoring Systems On The Support Structures Of Bottom-Fixed Offshore Wind, Mario Vieira, Brian Snyder, Elsa Henriques, Craig White, Luis Reis

Faculty Publications

Offshore wind (OSW) energy is a renewable source with strong prospects of development that may decisively contribute towards energy independence. Offshore wind is, however, not yet ubiquitously cost competitive, and frequently requires support schemes to finance its extensive capital requirements. Therefore, cost reduction strategies are necessary for the future development of offshore wind technologies. Even if structural health monitoring (SHM) systems are currently applied for the inspection of critical mechanical structures, they have not been the focus of research from offshore wind stakeholders. The main goal of this study is to evaluate the viability of SHM systems on the support …


Effects Of Alkylation On Potency Of Benz[A]Anthracene For Ahr2 Transactivation In Nine Species Of Freshwater Fish, Justin Dubiel, Hunter Johnson, Andreas N.M. Eriksson, A. Hontela, Jon A. Doering, Steve Wiseman Jul 2023

Effects Of Alkylation On Potency Of Benz[A]Anthracene For Ahr2 Transactivation In Nine Species Of Freshwater Fish, Justin Dubiel, Hunter Johnson, Andreas N.M. Eriksson, A. Hontela, Jon A. Doering, Steve Wiseman

Faculty Publications

Polycyclic aromatic hydrocarbons (PAHs) are naturally occurring or anthropogenic organic chemicals that can activate the aryl hydrocarbon receptor 2 (AhR2) and induce toxicity in fishes. Alkyl PAHs are more abundant than nonalkylated PAHs in certain environmental matrices and there is growing evidence that alkylation can increase potency, dependent on the position of alkylation. However, it is unknown if the effect of alkylation on potency is conserved across species. In addition, relatively little is known regarding the extent of interspecies variation in sensitivity to PAHs and alkyl PAHs. Therefore, objectives of the present study were to characterize potency of benz[a]anthracene (BAA) …


Transcription Factor Pecs Mediates Agrobacterium Fabrum Fitness And Survival, George C. Nwokocha, Prava Adhikari, Asif Iqbal, Hannah Elkholy, William T. Doerrler, John C. Larkin, Anne Grove Jul 2023

Transcription Factor Pecs Mediates Agrobacterium Fabrum Fitness And Survival, George C. Nwokocha, Prava Adhikari, Asif Iqbal, Hannah Elkholy, William T. Doerrler, John C. Larkin, Anne Grove

Faculty Publications

The transcriptional regulator PecS is encoded by select bacterial pathogens. For instance, in the plant pathogen Dickeya dadantii, PecS controls a range of virulence genes, including pectinase genes and the divergently oriented gene pecM, which encodes an efflux pump through which the antioxidant indigoidine is exported. In the plant pathogen Agrobacterium fabrum (formerly named Agrobacterium tumefaciens), the pecS-pecM locus is conserved. Using a strain of A. fabrum in which pecS has been disrupted, we show here that PecS controls a range of phenotypes that are associated with bacterial fitness. PecS represses flagellar motility and chemotaxis, which are processes that are …


Pastoral Leadership: A Catalyst For Whole-Church Ministry, Boubakar Sanou Jul 2023

Pastoral Leadership: A Catalyst For Whole-Church Ministry, Boubakar Sanou

Faculty Publications

No abstract provided.


Population-Specific Responses In Eastern Oysters Exposed To Low Salinity In The Northern Gulf Of Mexico, Kyle A. Sirovy, Sandra M. Casas, Jerome F. La Peyre, Morgan W. Kelly Jul 2023

Population-Specific Responses In Eastern Oysters Exposed To Low Salinity In The Northern Gulf Of Mexico, Kyle A. Sirovy, Sandra M. Casas, Jerome F. La Peyre, Morgan W. Kelly

Faculty Publications

Eastern oysters, Crassostrea virginica, are facing rapid environmental changes in the northern Gulf of Mexico and can respond to these changes via plasticity or evolution. Plastic responses can immediately buffer against environmental changes, although this buffering may impact the organism’s ability to evolve in subsequent generations. While plasticity and evolution are not mutually exclusive, the relative contribution and interaction between them remains unclear. In this study, we investigated the roles of plastic and evolved responses of C. virginica acclimated to low salinity using a common garden experiment with four populations exposed to two salinities. We used three transcriptomic analyses (edgeR, …