Digital Commons Network^™

Military-Related Exposures, Social Determinants Of Health, And Dysbiosis: The United States-Veteran Microbiome Project (Us-Vmp), Lisa A. Brenner, Andrew J. Hoisington, Kelly A. Stearns-Yoder, Christopher E. Stamper, Jared A. Heinze, Teodor T. Postolache, Daniel A. Hadidi, Claire A. Hoffmire, Maggie A. Stanislawski

Faculty Publications

Significant effort has been put forth to increase understanding regarding the role of the human microbiome in health- and disease-related processes. In turn, the United States (US) Veteran Microbiome Project (US-VMP) was conceptualized as a means by which to serially collect microbiome and health-related data from those seeking care within the Veterans Health Administration (VHA). In this manuscript, exposures related to military experiences, as well as conditions and health-related factors among patients seen in VHA clinical settings are discussed in relation to common psychological and physical outcomes. Upon enrollment in the study, Veterans complete psychometrically sound (i.e., reliable and valid) …

A Comparison Of Machine Learning Techniques For Taxonomic Classification Of Teeth From The Family Bovidae, Gregory J. Matthews, Juliet K. Brophy, Maxwell Luetkemeier, Hongie Gu, George K. Thiruvathukal

Faculty Publications

This study explores the performance of machine learning algorithms on the classification of fossil teeth in the Family Bovidae. Isolated bovid teeth are typically the most common fossils found in southern Africa and they often constitute the basis for paleoenvironmental reconstructions. Taxonomic identification of fossil bovid teeth, however, is often imprecise and subjective. Using modern teeth with known taxons, machine learning algorithms can be trained to classify fossils. Previous work by Brophy et al. [Quantitative morphological analysis of bovid teeth and implications for paleoenvironmental reconstruction of plovers lake, Gauteng Province, South Africa, J. Archaeol. Sci. 41 (2014), pp. 376–388] uses …

A Time Series Of Water Column Distributions And Sinking Particle Flux Of Pseudo-Nitzschia And Domoic Acid In The Santa Barbara Basin, California, Blaire P. Umhau, Claudia R. Benitez-Nelson, Clarissa R. Anderson, Kelly Mccabe, Christopher Burrell

Faculty Publications

Water column bulk Pseudo-nitzschia abundance and the dissolved and particulate domoic acid (DA) concentrations were measured in the Santa Barbara Basin (SBB), California from 2009–2013 and compared to bulk Pseudo-nitzschia cell abundance and DA concentrations and fluxes in sediment traps moored at 147 m and 509 m. Pseudo-nitzschia abundance throughout the study period was spatially and temporally heterogeneous (L⁻¹ to 3.8 × 10⁶ cells L⁻¹, avg. 2 × 10⁵ ± 5 × 10⁵ cells L⁻¹) and did not correspond with upwelling conditions or the total DA (tDA) concentration, which was also spatially …

Neonatal Vitamin A Injection Promotes Cattle Muscle Growth And Increases Oxidative Muscle Fibers, Bo Wang, Wei Nie, Xing Fu, Jeanene M. De Avila, Yannan Ma, Mei Jun Zhu, Martin Maquivar, Steven M. Parish, Jan R. Busboom, Mark L. Nelson, Min Du

Faculty Publications

© 2018 The Author(s). Background: Vitamin A and its metabolite, retinoic acid (RA), are important regulators of cell differentiation and organ morphogenesis. Its impact on beef cattle muscle growth remains undefined. Method: Angus steer calves were administrated with 0 (control) or 150,000IU vitamin A (retinyl palmitate in glycerol, i.m.) per calf at birth and 1 month of age. At 2 months of age, a biopsy of the Biceps femoris muscle was obtained to analyze the immediate effects of vitamin A injection on myogenic capacity of muscle cells. The resulting steers were harvested at 14months of age. Results: Vitamin A administration …

Recovery Of Phosphate And Dissolved Organic Matter From Aqueous Solution Using A Novel Cao-Mgo Hybrid Carbon Composite And Its Feasibility In Phosphorus Recycling, Ronghua Li, Jim J. Wang, Zengqiang Zhang, Mukesh Kumar Awasthi, Dan Du, Pengfei Dang, Qian Huang, Yichen Zhang, Lu Wang

Faculty Publications

Metal oxide-Carbon composites have been developed tailoring towards specific functionalities for removing pollutants from contaminated environmental systems. In this study, we synthesized a novel CaO-MgO hybrid carbon composite for removal of phosphate and humate by co-pyrolysis of dolomite and sawdust at various temperatures. Increasing of pyrolysis temperature to 900 °C generated a composite rich in carbon, CaO and MgO particles. Phosphate and humate can be removed efficiently by the synthesized composite with the initial solution in the range of pH 3.0–11.0. The phosphate adsorption was best fitted by pseudo-second-order kinetic model, while the humate adsorption followed the pseudo-second-order and the …

Reduced Carbon Use Efficiency And Increased Microbial Turnover With Soil Warming, Jianwei Li, Gangsheng Wang, Melanie A. Mayes, Steven D. Allison, Serita D. Frey, Zheng Shi, Xiao-Ming Hu, Yiqi Luo, Jerry M. Melillo

Faculty Publications

Global soil carbon (C) stocks are expected to decline with warming, and changes in microbial processes are key to this projection. However, warming responses of critical microbial parameters such as carbon use efficiency (CUE) and biomass turnover (rB) are not well understood. Here, we determine these parameters using a probabilistic inversion approach that integrates a microbial-enzyme model with 22 years of carbon cycling measurements at Harvard Forest. We find that increasing temperature reduces CUE but increases rB, and that two decades of soil warming increases the temperature sensitivities of CUE and rB. These temperature sensitivities, which are derived from decades-long …

Response To Turner 2017, Amy M. Schueller, Robert T. Leaf, Raymond M. Mroch Iii, Geneviève M. Nesslage

Faculty Publications

No abstract provided.

Carboxypeptidase O Is A Lipid Droplet-Associated Enzyme Able To Cleave Both Acidic And Polar C-Terminal Amino Acids, Linnea C. Burke, Hazel O. Ezeribe, Anna Y. Kwon, Donnell Dockery, Peter J. Lyons

Faculty Publications

Carboxypeptidase O (CPO) is a member of the M14 family of metallocarboxypeptidases with a preference for the cleavage of C-terminal acidic amino acids. CPO is largely expressed in the small intestine, although it has been detected in other tissues such as the brain and ovaries. CPO does not contain a prodomain, nor is it strongly regulated by pH, and hence appears to exist as a constitutively active enzyme. The goal of this study was to investigate the intracellular distribution and activity of CPO in order to predict physiological substrates and function. The distribution of CPO, when expressed in MDCK cells, …

Effects Of Pain Neuroscience Education On Physician Assistant Students Understanding Of Pain And Attitudes And Beliefs About Pain., Kory Zimney, Adriaan Louw, Julie Johnson, Suzanne Peppers, Kevin Farrell

Faculty Publications

PURPOSE: Evaluate the effectiveness and efficiency of two different pain neuroscience education (PNE) lectures provided to physician assistant (PA) students. Primary outcomes explored were knowledge of pain and shift in attitudes and beliefs about chronic pain after the lecture.

METHODS: A PNE lecture was provided at two separate university PA programs. One program received a two-hour PNE lecture with a case-based example. The other program received a one-hour PNE lecture without the casebased example. Measurement of change for pre and post-test pain knowledge and attitudes and beliefs about chronic pain were recorded.

RESULTS: Students at both universities showed medium effect …

Departmental Action Teams: A Five-Year Update On A Model For Sustainable Change, Daniel Reinholz, Mary Pilgrim, Karen Falkenberg, Courtney Ngai, Gina Quan, Sarah Wise, Chris Geanious, Joel Corbo, Noah Finkelstein

Faculty Publications

Fostering sustainable improvements in undergraduate education remains a formidable challenge. To address this challenge, our team has developed the Departmental Action Team (DAT) model. DATs are small working groups of faculty, students, and staff, that work collaboratively to envision, plan, develop, and build sustainable structures in their department. To support the uptake of such structures, DATs collect and analyze data to reflect on the root causes of an issue, which they use to shift beliefs, values, and practices within their context. This paper provides a five-year status report on the DAT project. We describe the history of the model, its …

Reactive Ion Plasma Modification Of Poly(Vinyl‐Alcohol) Increases Primary Endothelial Cell Affinity And Reduces Thrombogenicity, Patrick Jurney, Deirdre Anderson, Grace Pohan, Evelyn Yim, Monica Hinds

Faculty Publications

Bulk material properties and luminal surface interaction with blood determine the clinical viability of vascular grafts, and reducing intimal hyperplasia is necessary to improve their long‐term patency. Here, the authors report that the surface of a biocompatible hydrogel material, poly(vinyl alcohol) (PVA) can be altered by exposing it to reactive ion plasma (RIP) in order to increase primary endothelial cell attachment. The power and the carrier gas of the RIP treatment are varied and the resultant surface nitrogen, water contact angle, as well as the ability of the RIP‐treated surfaces to support primary endothelial colony forming cells is characterized. Additionally, …

Carbon Fixation: Closing The Circle, Marylou C. Machingura, James V. Moroney

Faculty Publications

© Machingura and Moroney. In Chlamydomonas the different stages of the Calvin-Benson cycle take place in separate locations within the chloroplast.

Loss Of Dbc1 (Ccar2) Affects Tnfα-Induced Lipolysis And Glut4 Gene Expression In Murine Adipocytes, Ashley A. Able, Allison J. Richard, Jacqueline M. Stephens

Faculty Publications

© 2018 Society for Endocrinology Published by Bioscientifica Ltd. STAT5A (signal transducer and activator of transcription 5A) is a transcription factor that plays a role in adipocyte development and function. In this study, we report DBC1 (deleted in breast cancer 1 - also known as CCAR2) as a novel STAT5A-interacting protein. DBC1 has been primarily studied in tumor cells, but there is evidence that loss of this protein may promote metabolic health in mice. Currently, the functions of DBC1 in mature adipocytes are largely unknown. Using immunoprecipitation and immunoblotting techniques, we confirmed that there is an association between endogenous STAT5A …

Dna Methylomes Of Bovine Gametes And In Vivo Produced Preimplantation Embryos, Zongliang Jiang, Jianan Lin, Hong Dong, Xinbao Zheng, Sadie L. Marjani, Jingyue Duan, Zhengqing Ouyang, Jingbo Chen, Xiuchun Tian

Faculty Publications

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. DNA methylation is an important epigenetic modification that undergoes dynamic changes in mammalian embryogenesis, during which both parental genomes are reprogrammed. Despite the many immunostaining studies that have assessed global methylation, the gene-specific DNA methylation patterns in bovine preimplantation embryos are unknown. Using reduced representation bisulfite sequencing, we determined genome-scale DNA methylation of bovine sperm and individual in vivo developed oocytes and preimplantation embryos. We show that (1) the major wave of genome-wide demethylation was completed by the 8-cell stage; (2) promoter …

An Ethanolic Extract Of Artemisia Scoparia Inhibits Lipolysis In Vivo And Has Antilipolytic Effects On Murine Adipocytes In Vitro, Anik Boudreau, Allison J. Richard, Jasmine A. Burrell, William T. King, Ruth Dunn, Jean Marc Schwarz, David M. Ribnicky, Jennifer Rood, J. Michael Salbaum, Jacqueline M. Stephens

Faculty Publications

© 2018, American Physiological Society. All rights reserved. An ethanolic extract of Artemisia scoparia (SCO) has metabolically favorable effects on adipocyte development and function in vitro and in vivo. In diet-induced obese mice, SCO supplementation significantly reduced fasting glucose and insulin levels. Given the importance of adipocyte lipolysis in metabolic health, we hypothesized that SCO modulates lipolysis in vitro and in vivo. Free fatty acids and glycerol were measured in the sera of mice fed a high-fat diet with or without SCO supplementation. In cultured 3T3-L1 adipocytes, the effects of SCO on lipolysis were assessed by measuring glycerol and free …

Maternal Encouragement Of Modest Behavior, Temperamental Shyness, And Anxious Withdrawal Linkages To Chinese American Children's Social Adjustment: A Moderated Mediation Analysis, Merve Balkaya, Charissa S.L. Cheah, Jing Yu, Craig H. Hart, Shuyan Sun

Faculty Publications

© 2018 John Wiley & Sons Ltd Past studies have revealed potential differences in the functional meaning and social evaluation of children's temperamental shyness between Chinese interdependence-oriented and North American independence-oriented cultural contexts. However, very little is known about shy Chinese American children's adjustment in Western school contexts and potential pathways underlying their adjustment. To address this gap in the literature, we examined the associations between Chinese American children's temperamental shyness and their social adjustment outcomes, including peer exclusion, prosocial behavior, and assertiveness/leadership skills. In addition, the mediating role of children's display of anxious-withdrawn behavior and the moderating role of …

Enhancer-Driven Alternative Promoters Of Imprinted Genes, Joomyeong Kim, Bambarendage P.U. Perera, Subash Ghimire

Faculty Publications

© 2018 Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. In the current study, we characterized the expression and histone modification profiles of the alternative promoters found within imprinted Igf2r, Mest, Zac1, Peg3, Snrpn and non-imprinted Myc loci. In terms of expression pattern, the alternative promoters are highly tissue-specific, which is in a stark contrast to the ubiquitous expression of the corresponding main promoters. The alternative promoters are associated with the …

Performance Of Bt-Susceptible And -Heterozygous Genotypes Of Spodoptera Frugiperda (J.E. Smith) Possessing Single- Or Dual-Gene Resistance Alleles In Sequential Feedings Of Non-Bt And Cry1a.105/Cry2ab2 Maize Leaf Tissues, Yiwan Zhou, Ying Niu, Graham P. Head, Paula A. Price, Fangneng Huang

Faculty Publications

We simulated larval feeding behavior in seed blends of non-Bt and Bt maize to determine if seed blends create more favorable conditions for heterozygous-resistant insects over their Bt-susceptible counterparts. Survival, growth, development, and progeny production of four genotypes of the fall armyworm, Spodoptera frugiperda, Bt-susceptible (aabb), Cry1A.105 heterozygous resistant (Aabb), Cry2Ab2 heterozygous resistant (aaBb), and Cry1A.105/Cry2Ab2 heterozygous resistant (AaBb), were evaluated in eight feeding sequences (Seq 1–8) of non-Bt and MON 89034 Bt maize leaf tissue expressing the Cry1A.105 and Cry2Ab2 proteins. We report variation in the performance of the four genotypes across the feeding sequences and biological parameters measured. …

Stress Fluctuation, Crack Renucleation And Toughening In Layered Materials, C. J. Hsueh, L. Avellar, B. Bourdin, G. Ravichandran, K. Bhattacharya

Faculty Publications

It has been established that contrast in the elastic properties can lead to enhancement of fracture toughness in heterogeneous materials. Focussing on layered materials as a model system, we show that this enhancement is a result of two distinct phenomena – first, fluctuations in stress leading to regions where the stress intensity at the crack is considerably smaller than that of the macroscopically applied value; and second, the lack of stress intensity when a crack is at a compliant to stiff interface thereby requiring renucleation. Using theoretical, computational and experimental methods, we study two geometries – a layered material and …

Anticyclonic Eddy Edge Effects On Phytoplankton Communities And Particle Export In The Northern South China Sea, Lei Wang, Bangqin Huang, Edward A. Laws, Kuanbo Zhou, Xin Liu, Yuyuan Xie, Minhan Dai

Faculty Publications

No abstract provided.

Anticyclonic Eddy Edge Effects On Phytoplankton Communities And Particle Export In The Northern South China Sea, Lei Wang, Bangqin Huang, Edward A. Laws, Kuanbo Zhou, Xin Liu, Yuyuan Xie, Minhan Dai

Faculty Publications

No abstract provided.

Return To The Repressive: Re-Thinking Nature- Culture In Contemporary Feminist Theory, Deborah Goldgaber

Faculty Publications

In "History of Sexuality"(Vol I.) Foucault argued that repression is the wrong model of power, understanding it in exclusively negative terms, as external to the body it constrains and inhibits. Power may also be positive, productive, and constitutive of the body and its possibilities. Thus, an adequate account of the relation between cultural forces and the body, Foucault argues, must challenge the "repressive hypothesis"(RH). Contemporary feminist accounts of the body are structured by this same oppositional view of power Foucault assumed: to call on Rosi Braidotti's distinction, discursive (cultural) forces are either negative or repressive (potestas) or positive and empowering …

Biological Effects On Uranium Isotope Fractionation (238U/235U) In Primary Biogenic Carbonates, Xinming Chen, Stephen J. Romaniello, Achim D. Herrmann, Elias Samankassou, Ariel D. Anbar

Faculty Publications

© 2018 Elsevier Ltd Determining whether U isotopes are fractionated during incorporation into biogenic carbonates could help to refine the application of 238U/235U in CaCO3 as a robust paleoredox proxy. Recent laboratory experiments have demonstrated that heavy uranium (U) isotopes were preferentially incorporated into abiotic aragonite, with an isotope fractionation of ∼0.10‰ (238U/235U). In contrast, no detectable U isotope fractionation has been observed in most natural primary biogenic carbonates, but the typical measurement precision of these studies was ±0.10‰ and so could not resolve a fractionation of the magnitude observed in the laboratory. To resolve this issue, we have developed …

The Gut Microbiome And Aquatic Toxicology: An Emerging Concept For Environmental Health, Ondrej Adamovsky, Amanda Buerger, Alexis M. Wormington, Naomi Ector, Robert J. Griffitt, Joseph H. Bisesi Jr., Christopher J. Martyniuk

Faculty Publications

The microbiome plays an essential role in the health and onset of diseases in all animals, including humans. The microbiome has emerged as a central theme in environmental toxicology, as microbes interact with the host immune system in addition to its role in chemical detoxification. Pathophysiological changes in the gastrointestinal tissue caused by ingested chemicals, and metabolites generated from microbial biodegradation, can lead to systemic adverse effects. This critical review dissects what we know about the impacts of environmental contaminants on the microbiome of aquatic species, with special emphasis on the gut microbiome. We highlight some of the known major …

Temporal Dynamics Of Lipid And Fatty Acid Characteristics Of Gulf Menhaden, Brevoortia Patronus In The Northern Gulf Of Mexico, Robert R. Leaf, Jesse Trushenski, Nancy Brown-Peterson, Michael J. Andres

Faculty Publications

Gulf Menhaden, Brevoortia patronus, in the northern Gulf of Mexico support a large commercial fishery and are thought to play an important trophic role in the coastal ecosystem. The temporal dynamics of both fatty acid and oil content have a direct impact on the value of Gulf Menhaden to predators and to the fishery. In this work, we describe how oil content of Gulf Menhaden varies with season, sex, age, condition, and tissue and investigate how fatty acid composition of mature (137.5 mm FL) female tissues varies with season, month, and tissue type. We found pronounced temporal (January to …

Oprm1 Genotype Interacts With Serotonin System Dysfunction To Predict Alcohol-Heightened Aggression In Primates, Carlos A. Driscoll, Stephen G. Lindell, Melanie L. Schwandt, Stephen J. Suomi, J. Dee Higley, Markus Heilig, Christina S. Barr

Faculty Publications

Although the notion that alcohol promotes violence is widespread, not all individuals are aggressive while intoxicated. Genetic variation could be a contributing factor to individual differences in alcohol-heightened aggression. The present study examines the effects of OPRM1C77G genotype on responses to threat in rhesus macaques under normal conditions and following alcohol administration. Prior studies have shown that a low CSF level of 5-HIAA is a trait marker for individuals prone to escalated aggression. We wanted to examine whether the predictive value for this marker on aggression was moderated by OPRM1 genotype. Animals were administered alcohol (BAC 100-200 mg%), were provoked …

Seascape Connectivity Of Gulf Sturgeon Acipenser Oxyrinchus Desotoi Population Units Across The Northern Gulf Of Mexico, Page E. Vick, Mark S. Peterson, William T. Slack

Faculty Publications

Critical habitat was designated in 2003 for federally threatened anadromous Gulf sturgeon to aid in population recovery. This study examined overwintering Gulf sturgeon spatial use and movement through critical habitat monitored by the Ship Island acoustic array from 2011 to 2015. Previous studies observed western population Gulf sturgeon (Pearl and Pascagoula rivers) overwintering near the ends and within the passes of the barrier islands of the Mississippi Sound, USA. Recent telemetry studies detected eastern population fish (Escambia, Blackwater, Yellow, and Choctawhatchee rivers) overwintering as far west as Mobile Bay, Alabama; however, this study is the first to observe eastern population …

An Investigation Of Engineering Design Cognition And Achievement In Primary School, Greg J. Strimel, Scott R. Bartholomew, Eunhye Kim, Liwei Zhang

Faculty Publications

This study examined the design cognition and achievement results of both kindergarten and fourth grade students engaged in engineering design-based instructional activities. Relationships between design cognition and student grade level, as well as quality of student work, were investigated. 30 concurrent think-aloud protocols were collected from individual primary students as they worked in groups to design and make a solution to a design task. The concurrent think-aloud protocols were examined and coded to determine the duration of time the participants devoted to a pre-established set of mental processes for technological problem solving. Significant differences between kindergarten and fourth grade participants …

Characterization Of Embyronic Stem Cell-Differentiated Fibroblasts As Mesenchymal Stem Cells With Robust Expansion Capacity And Attenuated Innate Immunity, William D'Angelo, Bohan Chen, Chandan Gurung, Yan-Lin Guo

Faculty Publications

Background: Mesenchymal stem cells (MSCs) isolated from adult tissues (Ad-MSCs) have shown great promise for use in regenerative medicine. However, their poor in vitro expansion capacity and tissue scarcity have been major limitations. In this study, we demonstrate that mouse embryonic stem cells (mESCs) can differentiate into cells with MSC properties.

Methods: Using previously established methods that characterize Ad-MSCs, we analyzed mESC-differentiated fibroblasts (mESC-FBs), including plastic adherence, clonogenic growth, MSC marker expression, tri-lineage differentiation potential, and the capacity to express immunomodulators.

Results: Although previously characterized as mESC-differentiated fibroblasts (mESC-FBs), these cells exhibit major properties of Ad-MSCs. However, …

Genetic And Psychological Factors Interact To Predict Physical Impairment Phenotypes Following Exercise-Induced Shoulder Injury, Paul A. Borsa, Jeffrey J. Parr, Margaret R. Wallace, Samuel S. Wu, Yunfeng Dai, Roger B. Fillingim, Steven Z. George

Faculty Publications

Background: We investigated interactions between genetic and psychological factors in predicting shoulder impairment phenotypes. We hypothesized that pro-inflammatory genes would display stronger relationships compared with pain-related genes when combined with psychological factors for predicting phenotypic changes.
Subjects and methods: Altogether, 190 participants completed a 5-day experimental protocol. An experimental shoulder injury model was used to induce physical impairment, and a priori selected genetic (pain-related, pro-inflammatory) and psychological (anxiety, depressive symptoms, pain catastrophizing, fear of pain, kinesiophobia) factors were included as predictors of interest. Impairment phenotypes were injury-induced deficits in range of motion (ROM) and strength. After controlling for age, sex, …