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Velocity-Resolved Reverberation Mapping Of Five Bright Seyfert 1 Galaxies, G. De Rosa, M. M. Fausnaugh, C. J. Grier, B. M. Peterson, K. D. Denney, Keith Horne, M. C. Bentz, S. Ciroi, Elena Dalla Bontà, M. D. Joner, S. Kaspi, C. S. Kochanek, R. W. Pogge, S. G. Sergeev, M. Vestergaard, S. M. Adams, J. Antognini, C. Araya Salvo, E. Armstrong, J. Bae, A. J. Barth, T. G. Beatty, A. Bhattacharjee, G. A. Borman, T. A. Boroson, M. C. Bottorff, J. E. Brown, J. S. Brown, M. S. Brotherton, C. T. Coker, C. Clanton, V. Cracco, S. M. Crawford Oct 2018

Velocity-Resolved Reverberation Mapping Of Five Bright Seyfert 1 Galaxies, G. De Rosa, M. M. Fausnaugh, C. J. Grier, B. M. Peterson, K. D. Denney, Keith Horne, M. C. Bentz, S. Ciroi, Elena Dalla Bontà, M. D. Joner, S. Kaspi, C. S. Kochanek, R. W. Pogge, S. G. Sergeev, M. Vestergaard, S. M. Adams, J. Antognini, C. Araya Salvo, E. Armstrong, J. Bae, A. J. Barth, T. G. Beatty, A. Bhattacharjee, G. A. Borman, T. A. Boroson, M. C. Bottorff, J. E. Brown, J. S. Brown, M. S. Brotherton, C. T. Coker, C. Clanton, V. Cracco, S. M. Crawford

Faculty Publications

We present the first results from a reverberation-mapping campaign undertaken during the first half of 2012, with additional data on one active galactic nucleus (AGN) (NGC 3227) from a 2014 campaign. Our main goals are (1) to determine the black hole masses from continuum-Hβ reverberation signatures, and (2) to look for velocity-dependent time delays that might be indicators of the gross kinematics of the broad-line region. We successfully measure Hβ time delays and black hole masses for five AGNs, four of which have previous reverberation mass measurements. The values measured here are in agreement with earlier estimates, though there is …


R1 Retrotransposons In The Nucleolar Organizers Of Drosophila Melanogaster Are Transcribed By Rna Polymerase I Upon Heat Shock, Himanshu S. Raje, Molly E. Lieux, Patrick J. Dimario Oct 2018

R1 Retrotransposons In The Nucleolar Organizers Of Drosophila Melanogaster Are Transcribed By Rna Polymerase I Upon Heat Shock, Himanshu S. Raje, Molly E. Lieux, Patrick J. Dimario

Faculty Publications

The ribosomal RNA genes (rDNA) of Drosophila melanogaster reside within centromere-proximal nucleolar organizers on both the X and Y chromosomes. Each locus contains between 200–300 tandem repeat rDNA units that encode 18S, 5.8S, 2S, and 28S ribosomal RNAs (rRNAs) necessary for ribosome biogenesis. In arthropods like Drosophila, about 60% of the rDNA genes have R1 and/or R2 retrotransposons inserted at specific sites within their 28S regions; these units likely fail to produce functional 28S rRNA. We showed earlier that R2 expression increases upon nucleolar stress caused by the loss of the ribosome assembly factor, Nucleolar Phosphoprotein of 140 kDa (Nopp140). …


Proton Therapy For Adults With Mediastinal Lymphomas: The International Lymphoma Radiation Oncology Group Guidelines, Bouthaina Shbib Dabaja, Bradford S. Hoppe, John P. Plastaras, Wayne Newhauser, Katerina Rosolova, Stella Flampouri, Radhe Mohan, N. George Mikhaeel, Youlia Kirova, Lena Specht, Joachim Yahalom Oct 2018

Proton Therapy For Adults With Mediastinal Lymphomas: The International Lymphoma Radiation Oncology Group Guidelines, Bouthaina Shbib Dabaja, Bradford S. Hoppe, John P. Plastaras, Wayne Newhauser, Katerina Rosolova, Stella Flampouri, Radhe Mohan, N. George Mikhaeel, Youlia Kirova, Lena Specht, Joachim Yahalom

Faculty Publications

Among adult lymphoma survivors, radiation treatment techniques that increase the excess radiation dose to organs at risk (OARs) put patients at risk for increased side effects, especially late toxicities. Minimizing radiation to OARs in adults patients with Hodgkin and non-Hodgkin lymphomas involving the mediastinum is the deciding factor for the choice of treatment modality. Proton therapy may help to reduce the radiation dose to the OARs and reduce toxicities, especially the risks for cardiac morbidity and second cancers. Becauseproton therapymay have some disadvantages, identifying the patients and the circumstances that may benefit the most from proton therapy is important. We …


Metallo-Polyelectrolytes As A Class Of Ionic Macromolecules For Functional Materials, Tianyu Zhu, Ye Sha, Jing Yan, Parasmani Pageni, Md Anisur Rahman, Yi Yan, Chuanbing Tang Oct 2018

Metallo-Polyelectrolytes As A Class Of Ionic Macromolecules For Functional Materials, Tianyu Zhu, Ye Sha, Jing Yan, Parasmani Pageni, Md Anisur Rahman, Yi Yan, Chuanbing Tang

Faculty Publications

The fields of soft polymers and macromolecular sciences have enjoyed a unique combination of metals and organic frameworks in the name of metallopolymers or organometallic polymers. When metallopolymers carry charged groups, they form a class of metal-containing polyelectrolytes or metallo-polyelectrolytes. This review identifies the unique properties and functions of metallo-polyelectrolytes compared with conventional organo-polyelectrolytes, in the hope of shedding light on the formation of functional materials with intriguing applications and potential benefits. It concludes with a critical perspective on the challenges and hurdles for metallo-polyelectrolytes, especially experimental quantitative analysis and theoretical modeling of ionic binding.


Evidence That A Respiratory Shield In Escherichia Coli Protects A Low-Molecular-Mass Fe-Ii Pool From O2-Dependent Oxidation, Joshua D. Wofford, Naimah Bolaji, Nathaniel Dziuba, Franklin Wayne Outten, Paul A. Lindahl Oct 2018

Evidence That A Respiratory Shield In Escherichia Coli Protects A Low-Molecular-Mass Fe-Ii Pool From O2-Dependent Oxidation, Joshua D. Wofford, Naimah Bolaji, Nathaniel Dziuba, Franklin Wayne Outten, Paul A. Lindahl

Faculty Publications

Iron is critical for virtually all organisms, yet major questions remain regarding the systems' level understanding of iron in whole cells. Here, we obtained Mössbauer and EPR spectra of Escherichia coli cells prepared under different nutrient iron concentrations, carbon sources, growth phases, and O2 concentrations to better understand their global iron content. We investigated wild-type cells and those lacking Fur, FtnA, Bfr, and Dps proteins. The coarse-grain iron content of exponentially growing cells consisted of iron-sulfur clusters, variable amounts of nonheme high-spin FeII species, and an unassigned residual quadrupole doublet. The iron in stationary-phase cells was dominated by …


Hydrological Connectivity In Vegetated River Deltas: The Importance Of Patchiness Below A Threshold, Kyle Wright, Matthew Hiatt, Paola Passalacqua Oct 2018

Hydrological Connectivity In Vegetated River Deltas: The Importance Of Patchiness Below A Threshold, Kyle Wright, Matthew Hiatt, Paola Passalacqua

Faculty Publications

No abstract provided.


Galveston Bay And Coastal Ocean Optical-Geochemical Response To Hurricane Harvey From Viirs Ocean Color, Eurico J. D’Sa, Ishan Joshi, Bingqing Liu Oct 2018

Galveston Bay And Coastal Ocean Optical-Geochemical Response To Hurricane Harvey From Viirs Ocean Color, Eurico J. D’Sa, Ishan Joshi, Bingqing Liu

Faculty Publications

Dissolved and particulate organic carbon, suspended particulate matter concentrations, and their optical proxies colored dissolved organic matter absorption and backscattering coefficients were studied in Galveston Bay, Texas, following the extreme flooding of Houston and surrounding areas due to Hurricane Harvey (25–29 August 2017) using field and ocean color observations. A three-step empirical-semianalytic algorithm for determination of colored dissolved organic matter absorption and backscattering coefficients revealed the dynamics of dissolved organic carbon and particle distribution from Visible and Infrared Imaging Radiometric Suite ocean color. Environmental drivers, especially floodwater discharge and winds, strongly influenced the spatiotemporal distribution of dissolved/particulate material in the …


A Simple, Sensitive And Reliable Lc-Ms/Ms Method For The Determination Of 7-Bromo-5-Chloroquinolin-8-Ol (Clbq14), A Potent And Selective Inhibitor Of Methionine Aminopeptidases: Application To Pharmacokinetic Studies, Oscar Ekpenyong, Candace Cooper, Jing Ma, Dong Liang, Omonike Olaleye, Huan Xie Oct 2018

A Simple, Sensitive And Reliable Lc-Ms/Ms Method For The Determination Of 7-Bromo-5-Chloroquinolin-8-Ol (Clbq14), A Potent And Selective Inhibitor Of Methionine Aminopeptidases: Application To Pharmacokinetic Studies, Oscar Ekpenyong, Candace Cooper, Jing Ma, Dong Liang, Omonike Olaleye, Huan Xie

Faculty Publications

CLBQ14 is an 8-hydroxyquinoline analogue that inhibits methionine aminopeptidase (MetAP), an enzyme responsible for the post-translational modification of several proteins and polypeptides. MetAP has been validated as druggable target for some infectious diseases, and its inhibitors have been investigated as potential therapeutic agents. In this study, we developed and validated a liquid chromatography tandem-mass spectrometry (LC-MS/MS) method for the quantification of CLBQ14 in solution, and in rat plasma and urine. This method was applied to the pharmacokinetic evaluation of CLBQ14 in adult male Sprague Dawley (SD) rats. Chromatographic separation was achieved using an ultra-high-performance liquid chromatography (UHPLC) system equipped with …


Aldosterone Mediated Regulation Of Epithelial Sodium Channel (Enac) Subunits In The Rat Hypothalamus, Natalie J. Mills, Kaustubh Sharma, Masudul Haque, Meagan Moore, Ryoichi Teruyama Oct 2018

Aldosterone Mediated Regulation Of Epithelial Sodium Channel (Enac) Subunits In The Rat Hypothalamus, Natalie J. Mills, Kaustubh Sharma, Masudul Haque, Meagan Moore, Ryoichi Teruyama

Faculty Publications

© 2018 IBRO Current evidence suggests that the epithelial Na + channel (ENaC) in the brain plays a significant role in the development of hypertension. ENaC is present in vasopressin (VP) neurons in the hypothalamus, suggesting that ENaC in VP neurons is involved in the regulation of blood pressure. Our recent study demonstrated that high dietary salt intake caused an increase in the expression and activity of ENaC that were responsible for the more depolarized basal membrane potential in VP neurons. A known regulator of ENaC expression, the mineralocorticoid receptor (MR), is present in VP neurons, suggesting that ENaC expression …


Linear Bidentate Ligands (L) With Two Terminal Pyridyl N-Donor Groups Forming Pt(Ii)Lcl2 Complexes With Rare Eight-Membered Chelate Rings, Kokila Ranasinghe, Patricia A. Marzilli, Svetlana Pakhomova, Luigi G. Marzilli Oct 2018

Linear Bidentate Ligands (L) With Two Terminal Pyridyl N-Donor Groups Forming Pt(Ii)Lcl2 Complexes With Rare Eight-Membered Chelate Rings, Kokila Ranasinghe, Patricia A. Marzilli, Svetlana Pakhomova, Luigi G. Marzilli

Faculty Publications

Copyright © 2018 American Chemical Society. NMR and X-ray diffraction studies were conducted on Pt(II)LCl2 complexes prepared with the new N-donor ligands N(SO2R)Mendpa (R = Me, Tol; n = 2, 4). These ligands differ from N(H)dpa (di-2-picolylamine) in having the central N within a tertiary sulfonamide group instead of a secondary amine group and having Me groups at the 6,6′-positions (n = 2) or 3,3′,5,5′-positions (n = 4) of the pyridyl rings. The N(SO2R)3,3′,5,5′-Me4dpa ligands are coordinated in a bidentate fashion in Pt(N(SO2R)3,3′,5,5′-Me4dpa)Cl2 complexes, forming a rare eight-membered chelate ring. The sulfonamide N atom did not bind to Pt(II), consistent …


Cip2a Immunosensor Comprised Of Vertically-Aligned Carbon Nanotube Interdigitated Electrodes Towards Point-Of-Care Oral Cancer Screening, Shaowei Ding, Suprem R. Das, Benjamin J. Brownlee, Kshama Parate, Taylor Davis, Loreen R. Stromberg, Edward K.L. Chan, Joseph Katz, Brian D. Iverson, Jonathan C. Claussen Oct 2018

Cip2a Immunosensor Comprised Of Vertically-Aligned Carbon Nanotube Interdigitated Electrodes Towards Point-Of-Care Oral Cancer Screening, Shaowei Ding, Suprem R. Das, Benjamin J. Brownlee, Kshama Parate, Taylor Davis, Loreen R. Stromberg, Edward K.L. Chan, Joseph Katz, Brian D. Iverson, Jonathan C. Claussen

Faculty Publications

Vertically aligned carbon nanotube array (VANTA) coatings have recently garnered much attention due in part to their unique material properties including light absorption, chemical inertness, and electrical conductivity. Herein we report the first use of VANTAs grown via chemical vapor deposition in a 2D interdigitated electrode (IDE) footprint with a high height-to-width aspect ratio (3:1 or 75:25 µm). The VANTA-IDE is functionalized with an antibody (Ab) specific to the human cancerous inhibitor PP2A (CIP2A)—a alivary oncoprotein that is associated with a variety of malignancies such as oral, breast, and multiple myeloma cancers. The resultant immunosensor is capable of detecting CIP2A …


A Mobile Smoking Cessation Intervention For Mexico (Vive Sin Tabaco... ¡Decídete!): Single-Arm Pilot Study, Ana Paula Cupertino, Francisco Cartujano-Barrera, Mariana Ramírez, Rosibel Rodríguez-Bolaños, James F. Thrasher, Gloria Pérez-Rubio, Ramcés Falfán-Valencia, Edward F. Ellerbeck, Luz Myriam Reynales-Shigematsu Oct 2018

A Mobile Smoking Cessation Intervention For Mexico (Vive Sin Tabaco... ¡Decídete!): Single-Arm Pilot Study, Ana Paula Cupertino, Francisco Cartujano-Barrera, Mariana Ramírez, Rosibel Rodríguez-Bolaños, James F. Thrasher, Gloria Pérez-Rubio, Ramcés Falfán-Valencia, Edward F. Ellerbeck, Luz Myriam Reynales-Shigematsu

Faculty Publications

Background: Of the 14.3 million Mexicans who smoke, only a minority take advantage of evidence-based approaches to smoking cessation. Mobile health interventions have the potential to increase the reach of effective cessation interventions in Mexico.

Objective: This study aimed to assess the feasibility and acceptability of an innovative, personalized, and interactive smoking cessation mobile intervention developed for Mexican smokers.

Methods: We recruited 40 Mexican smokers to participate in Vive sin Tabaco... ¡Decídete!, a smoking cessation program that uses a tablet-based decision support software to drive a 12-week text messaging smoking cessation program and pharmacotherapy support. Outcome measures included participant text …


Effects Of Coumaphos And Imidacloprid On Honey Bee (Hymenoptera: Apidae) Lifespan And Antioxidant Gene Regulations In Laboratory Experiments, Ales Gregorc, Mohamed Alburaki, Nicholas Rinderer, Blair Sampson, Patricia R. Knight, Shahid Karim, John Adamczyk Oct 2018

Effects Of Coumaphos And Imidacloprid On Honey Bee (Hymenoptera: Apidae) Lifespan And Antioxidant Gene Regulations In Laboratory Experiments, Ales Gregorc, Mohamed Alburaki, Nicholas Rinderer, Blair Sampson, Patricia R. Knight, Shahid Karim, John Adamczyk

Faculty Publications

The main objective of this study was to test comparatively the effects of two common insecticides on honey bee Apis mellifera worker’s lifespan, food consumption, mortality, and expression of antioxidant genes. Newly emerged worker bees were exposed to organophosphate insecticide coumaphos, a neonicotinoid imidacloprid, and their mixtures. Toxicity tests were conducted along with bee midgut immunohistological TUNEL analyses. RT-qPCR assessed the regulation of 10 bee antioxidant genes linked to pesticide toxicity. We tested coumaphos at 92,600 ppb concentration, in combination with 5 and 20 ppb imidacloprid. Coumaphos induced significantly higher bee mortality, which was associated with down regulation of catalase …


Oxygen Deprivation Influences The Survival Of Listeria Monocytogenes In Gerbils, Jillian Harris, Oindrila Paul, Si Hong Park, Sally White, Khemraj Budachetri, Daniel M. Mcclung, Jessica G. Wilson, Alicia K. Olivier, Justin A. Thornton, Paul R. Broadway, Steven C. Ricke, Janet R. Donaldson Oct 2018

Oxygen Deprivation Influences The Survival Of Listeria Monocytogenes In Gerbils, Jillian Harris, Oindrila Paul, Si Hong Park, Sally White, Khemraj Budachetri, Daniel M. Mcclung, Jessica G. Wilson, Alicia K. Olivier, Justin A. Thornton, Paul R. Broadway, Steven C. Ricke, Janet R. Donaldson

Faculty Publications

Listeria monocytogenes is a facultative anaerobic foodborne pathogen capable of surviving harsh environments. Recent work has indicated that anaerobic conditions increase the resistance capability of certain strains to environmental stressors. The goal of the study was to conduct a preliminary study to determine whether exposure to anaerobic conditions prior to infection increases the ability to survive in vivo. Gerbils were inoculated with one of five doses of the L. monocytogenes strain F2365 by oral gavage: phosphate-buffered saline (control), 5 × 106 colony forming units aerobic culture (low aerobic), 5 × 108 aerobic culture (high aerobic), 5 × 10 …


Fibroblast Growth Factor 21, Adiposity, And Macronutrient Balance In A Healthy, Pregnant Population With Overweight And Obesity, Elizabeth F. Sutton, Christopher D. Morrison, Jacqueline M. Stephens, Leanne M. Redman Oct 2018

Fibroblast Growth Factor 21, Adiposity, And Macronutrient Balance In A Healthy, Pregnant Population With Overweight And Obesity, Elizabeth F. Sutton, Christopher D. Morrison, Jacqueline M. Stephens, Leanne M. Redman

Faculty Publications

© 2018, © 2018 Taylor & Francis. Aim of the study: The regulation and actions of fibroblast growth factor 21 (FGF21) are responsive to energy status and macronutrient balance, and investigations of FGF21 in normal pregnancy, which could be informative for FGF21 biology, are seldom. The goal of our study was to examine FGF21 levels in a contemporary healthy, pregnant population. Methods: We phenotyped 43 women with overweight and obesity during pregnancy for weight, body composition, and fasting blood. Serum FGF21 was measured during the first and third trimesters. Placentas were collected at delivery. Results: Maternal FGF21 concentrations were positively …


Microbial Community Structure And Functional Potential Of Lava-Formed Gotjawal Soils In Jeju, Korea, Jong Shik Kim, Dae Shin Kim, Keun Chul Lee, Jung Sook Lee, Gary M. King, Sanghoon Kang Oct 2018

Microbial Community Structure And Functional Potential Of Lava-Formed Gotjawal Soils In Jeju, Korea, Jong Shik Kim, Dae Shin Kim, Keun Chul Lee, Jung Sook Lee, Gary M. King, Sanghoon Kang

Faculty Publications

© 2018 Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The Gotjawal areas of Jeju Island, Korea, are comprised of unmanaged forests growing on volcanic soils. They support unique assemblages of vascular plants from both northern and southern hemispheres, but are threatened by human disturbance. The health and ecosystem function of these assemblages likely depends in part on the diversity and community structure of soil microbial communities, about which little is …


3d Mri Modeling Of Thin And Spatially Complex Soft Tissue Structures Without Shrinkage: Lamprey Myosepta As An Example, Bradley M. Wood, Guang Jia, Owen Carmichael, Kevin Mcklveen, Dominique G. Homberger Oct 2018

3d Mri Modeling Of Thin And Spatially Complex Soft Tissue Structures Without Shrinkage: Lamprey Myosepta As An Example, Bradley M. Wood, Guang Jia, Owen Carmichael, Kevin Mcklveen, Dominique G. Homberger

Faculty Publications

© 2018 Wiley Periodicals, Inc. 3D imaging techniques enable the nondestructive analysis and modeling of complex structures. Among these, MRI exhibits good soft tissue contrast, but is currently less commonly used for nonclinical research than X-ray CT, even though the latter requires contrast-staining that shrinks and distorts soft tissues. When the objective is the creation of a realistic and complete 3D model of soft tissue structures, MRI data are more demanding to acquire and visualize and require extensive post-processing because they comprise noncubic voxels with dimensions that represent a trade-off between tissue contrast and image resolution. Therefore, thin soft tissue …


Spreading The News About The Novel Conjugation Mechanism In Streptomyces Bacteria, Gregg S. Pettis Oct 2018

Spreading The News About The Novel Conjugation Mechanism In Streptomyces Bacteria, Gregg S. Pettis

Faculty Publications

© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd The hallmark of mycelial spore-forming bacteria of the genus Streptomyces is their prolific production of antibiotics and other bioactive secondary metabolites as part of a complex morphological and physiological developmental program. They are further distinguished by a conjugation mechanism that differs substantially from the single-strand mode of DNA transfer via Type IV secretion, which is exhibited by numerous unicellular Gram-negative and Gram-positive bacteria. At the crux of the novel intermycelial transfer event in Streptomyces spp. is a membrane pore composed of a single plasmid protein (TraB), which also …


Particulate Matter Containing Environmentally Persistent Free Radicals Induces Ahrdependent Cytokine And Reactive Oxygen Species Production In Human Bronchial Epithelial Cells, Ashlyn C. Harmon, Valeria Y. Hebert, Stephania A. Cormier, Balamurugan Subramanian, James R. Reed, Wayne L. Backes, Tammy R. Dugas Oct 2018

Particulate Matter Containing Environmentally Persistent Free Radicals Induces Ahrdependent Cytokine And Reactive Oxygen Species Production In Human Bronchial Epithelial Cells, Ashlyn C. Harmon, Valeria Y. Hebert, Stephania A. Cormier, Balamurugan Subramanian, James R. Reed, Wayne L. Backes, Tammy R. Dugas

Faculty Publications

© 2018 Harmon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Particulate matter (PM) is emitted during the combustion of fuels and wastes. PM exposure exacerbates pulmonary diseases, and the mechanism may involve oxidative stress. At lower combustion temperatures such as occurs in the cool zone of a flame, aromatic compounds chemisorb to the surface of metal-oxide-containing PM, resulting in the formation of surface-stabilized environmentally persistent free radicals (EPFR). Prior studies showed …


Paper Promises For Drug Innovation, Erika Lietzan Oct 2018

Paper Promises For Drug Innovation, Erika Lietzan

Faculty Publications

Innovation does not stop when a new medicine is launched. Development of new uses for already approved drugs, in particular, can make profound contributions to the public health. Whether a new use is suspected during the initial premarket trials, identified through focused research after approval, or discovered serendipitously by physicians treating patients, however, it requires extensive clinical testing before it can be approved by FDA. This testing takes time and money — three to five years on average, and as much as $300 million. This Article considers the incentives that federal law offers to companies to make this investment: patent …


Class Specific Discrimination Of Volatile Organic Compounds Using A Quartz Crystal Microbalance Based Multisensor Array, Stephanie R. Vaughan, Nicholas C. Speller, Pratap Chhotaray, Kevin S. Mccarter, Noureen Siraj, Rocío L. Pérez, Yue Li, Isiah M. Warner Oct 2018

Class Specific Discrimination Of Volatile Organic Compounds Using A Quartz Crystal Microbalance Based Multisensor Array, Stephanie R. Vaughan, Nicholas C. Speller, Pratap Chhotaray, Kevin S. Mccarter, Noureen Siraj, Rocío L. Pérez, Yue Li, Isiah M. Warner

Faculty Publications

© 2018 Elsevier B.V. The use of quartz crystal microbalance (QCM) sensor arrays for analyses of volatile organic compounds (VOC) has attracted significant interest in recent years. In this regard, a group of uniformed materials based on organic salts (GUMBOS) has proven to be promising recognition elements in QCM based sensor arrays due to diverse properties afforded by this class of tunable materials. Herein, we examine the application of four novel phthalocyanine based GUMBOS as recognition elements for VOC sensing using a QCM based multisensor array (MSA). These synthesized GUMBOS are composed of copper (II) phthalocyaninetetrasulfonate (CuPcS4) anions coupled with …


Community Phylogeny Of The Globally Critically Imperiled Pine Rockland Ecosystem, Lauren B. Trotta, Benjamin Baiser, Jennifer Possley, Daijiang Li, James Lange, Sarah Martin, Emily B. Sessa Oct 2018

Community Phylogeny Of The Globally Critically Imperiled Pine Rockland Ecosystem, Lauren B. Trotta, Benjamin Baiser, Jennifer Possley, Daijiang Li, James Lange, Sarah Martin, Emily B. Sessa

Faculty Publications

© 2018 Botanical Society of America Premise of the Study: Community phylogenetic methods incorporate information on evolutionary relationships into studies of organismal assemblages. We used a community phylogenetic framework to investigate relationships and biogeographic affinities and to calculate phylogenetic signal of endemism and invasiveness for the flora of the pine rocklands—a globally critically imperiled ecosystem with a significant portion of its distribution in South Florida, United States. Methods: We reconstructed phylogenetic relationships of 538 vascular plant taxa, which represent 92.28% of the vascular flora of the pine rocklands. We estimated phylogenetic signal for endemism and invasiveness using phylogenetic generalized linear …


Fertilization Influences The Nutrient Acquisition Strategy Of A Nomadic Vine In A Lowland Tropical Forest Understory, Carrie L. Woods, Saara J. Dewalt, Catherine L. Cardelús, Kyle E. Harms, Joseph B. Yavitt, S. Joseph Wright Oct 2018

Fertilization Influences The Nutrient Acquisition Strategy Of A Nomadic Vine In A Lowland Tropical Forest Understory, Carrie L. Woods, Saara J. Dewalt, Catherine L. Cardelús, Kyle E. Harms, Joseph B. Yavitt, S. Joseph Wright

Faculty Publications

© 2018, Springer Nature Switzerland AG. Aims: Tropical tree and lianas in the understory are limited by soil nutrients despite growing in extremely low light. It is not known if nomadic vines are also limited by nutrients in low light conditions. Methods: We measured differences in root architecture and mycorrhizal colonization, and leaf nutrients of a nomadic vine, Philodendron fragrantissimum (Araceae), in nitrogen (N) and phosphorus (P) fertilization plots in a lowland tropical moist forest in central Panama to measure potential nutrient limitation. Results: Relative to plants in control plots, leaf P concentration was 54% higher and leaf N concentration …


Using A Phic31 "Disintegrase" To Make New Attp Sites In The Drosophila Genome At Locations Showing Chromosomal Position Effects, Mukesh Maharjan, Robert K. Maeda, François Karch, Craig M. Hart Oct 2018

Using A Phic31 "Disintegrase" To Make New Attp Sites In The Drosophila Genome At Locations Showing Chromosomal Position Effects, Mukesh Maharjan, Robert K. Maeda, François Karch, Craig M. Hart

Faculty Publications

© 2018 Maharjan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. An engineered phiC31 "Disintegrase" able to make an attP site in Drosophila out of an attRattL pair is described. This was used to generate attP sites at genomic locations where a mini-white (mini-w) transgene was subject to chromosomal position effects (CPE). The first step was random genomic integration of a P-element-based transposon with an insulated mini-w transgene. We then removed the …


Trans-Allelic Mutational Effects At The Peg3 Imprinted Locus, Corey L. Bretz, Joomyeong Kim Oct 2018

Trans-Allelic Mutational Effects At The Peg3 Imprinted Locus, Corey L. Bretz, Joomyeong Kim

Faculty Publications

© 2018 Bretz, Kim. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. How one allele interacts with the other for the function of a gene is not well understood. In this study, we tested potential allelic interaction at the Peg3 imprinted locus with several mutant alleles targeting an Imprinting Control Region, the Peg3-DMR. According to the results, maternal deletion of the Peg3-DMR resulted in 2-fold up-regulation of two paternally expressed genes, Peg3 and Usp29. …


An Overview Of Factors Affecting Distribution Of The Atlantic Surfclam (Spisula Solidissima), A Continental Shelf Biomass Dominant, During A Period Of Climate Change, Eileen E. Hoffman, Eric N. Powell, John M. Klink, Daphne M. Munroe, Roger Mann, Dale B. Haidvogel, Diego A. Narváez, Xinzhong Zhang, Kelsey M. Kuykendall Oct 2018

An Overview Of Factors Affecting Distribution Of The Atlantic Surfclam (Spisula Solidissima), A Continental Shelf Biomass Dominant, During A Period Of Climate Change, Eileen E. Hoffman, Eric N. Powell, John M. Klink, Daphne M. Munroe, Roger Mann, Dale B. Haidvogel, Diego A. Narváez, Xinzhong Zhang, Kelsey M. Kuykendall

Faculty Publications

The Atlantic surfclam (Spisula solidissima) is a dominant member of the biological community of the Middle Atlantic Bight continental shelf and a commercially harvested species. Climate warming is affecting the biology and distribution of this species, which provides an opportunity to investigate the processes and conditions that are restructuring this fishery and the implications for ecological and socioeconomic systems. A Management Strategy Evaluation (MSE) developed for the surfclam fishery provides a mechanistic description of the surfclam's response to climate change and understanding of the cascade of effects initiated by changes in oceanographic conditions that ultimately appear as social and economic …


Design And Cost Analysis Of A Self-Contained Mobile Laboratory For Commercial-Scale Aquatic Species Cryopreservation, William M. Childress, Rex H. Caffey, Terrence R. Tiersch Oct 2018

Design And Cost Analysis Of A Self-Contained Mobile Laboratory For Commercial-Scale Aquatic Species Cryopreservation, William M. Childress, Rex H. Caffey, Terrence R. Tiersch

Faculty Publications

© Copyright by the World Aquaculture Society 2018 Although aquatic species cryopreservation protocols have been studied around the world over the past 60 yr., germplasm repository development efforts and commercialization have begun only recently. The goal of this project was to develop a self-contained mobile laboratory for on-site high-throughput cryopreservation of aquatic species. The objectives of this study were to: (1) identify how a mobile laboratory would function in different operational scenarios, (2) customize an enclosed cargo trailer to function as a mobile laboratory, (3) evaluate the laboratory layout and ability of cryopreservation equipment to operate from generator power, and …


Social Class Divergence In Family Transitions: The Importance Of Cohabitation, Heather M. Rackin, Christina M. Gibson-Davis Oct 2018

Social Class Divergence In Family Transitions: The Importance Of Cohabitation, Heather M. Rackin, Christina M. Gibson-Davis

Faculty Publications

Objective: This study examined trends in familial transitions by maternal education and whether transitions rose because of changes in prevalence (the share of children exposed to a relationship state, either marriage or cohabitation) or churning (the number of entrances and exits conditional on being exposed to a relationship state). Background: Children's experiences of transitions, an important predictor of well-being, have leveled off in recent decades. Plateauing in transitions may reflect heterogeneity by socioeconomic status. Method: Data came from the National Survey of Family Growth on firstborn children observed from ages 0 to 5 among mothers aged 15 to 34 at …


Measurement And Modeling Of The Spatiotemporal Dynamics Of Beach Surface Moisture Content, Phillip P. Schmutz, Steven L. Namikas Oct 2018

Measurement And Modeling Of The Spatiotemporal Dynamics Of Beach Surface Moisture Content, Phillip P. Schmutz, Steven L. Namikas

Faculty Publications

The spatiotemporal dynamics of surface moisture exert a significant influence on the operation of aeolian transport systems at many beaches. However, we currently lack the detailed understanding of variability in surface moisture required to incorporate it into aeolian models. This problem is addressed here through direct measurements and simulation modeling of beach surface moisture over a twelve-day period, and through quantification of the relative contributions of evaporation, condensation and groundwater inputs to surface moisture. It was found that the beach surface can be characterized spatially in terms of three moisture zones: a consistently dry zone (40%). The relative influence of …


Detrital Nutrient Content And Leaf Species Differenitally Affect Growth And Nutritional Regulation Of Detritivores, Halvor M. Halvorson, Chris L. Fuller, Sally A. Entrekin, J. Thad Scott, Michelle A. Evans-White Oct 2018

Detrital Nutrient Content And Leaf Species Differenitally Affect Growth And Nutritional Regulation Of Detritivores, Halvor M. Halvorson, Chris L. Fuller, Sally A. Entrekin, J. Thad Scott, Michelle A. Evans-White

Faculty Publications

© 2018 The Authors Resource nutrient content and identity are common bottom–up controls on organismal growth and nutritional regulation. One framework to study these factors, ecological stoichiometry theory, predicts that elevated resource nitrogen (N) and phosphorus (P) contents enhance organism growth by alleviating constraints on N and P acquisition. However, the regulatory mechanisms underlying this response – including whether responses depend on resource identity – remain poorly understood. In this study, we tested roles of detrital N and P contents and identity (leaf species) in constraining growth of aquatic invertebrate detritivores. We synthesized results from seven detritivore species fed wide …