Digital Commons Network^™

Validation Of Commercial Dna Tests For Quantitative Beef Quality Traits, A. L. Van Eenennaam, J. Li, R. M. Thallman, R. L. Quaas, M. E. Dikeman, C. A. Gill, D. E. Franke, M. G. Thomas

Faculty Publications

Associations between 3 commercially available genetic marker panels (GeneSTAR Quality Grade, GeneSTAR Tenderness, and Igenity Tender-GENE) and quantitative beef traits were validated by the US National Beef Cattle Evaluation Consortium. Validation was interpreted to be the independent confirmation of the associations between genetic tests and phenotypes, as claimed by the commercial genotyping companies. Validation of the quality grade test (GeneSTAR Quality Grade) was carried out on 400 Charolais x Angus crossbred cattle, and validation of the tenderness tests (GeneSTAR Tenderness and Igenity Tender-GENE) was carried out on over 1,000 Bos taurus and Bos indicus cattle. The GeneSTAR Quality Grade marker …

Using Public Health Indicators To Measure The Success Of Hypertension Control, Richard S. Cooper

Faculty Publications

No abstract provided.

Gstm1, Gstt1, Gstp1, And Gsta1 Polymorphisms And Urinary Isothiocyanate Metabolites Following Broccoli Consumption In Humans, Susan E. Steck, Marilie D. Gammon, James R. Hébert, Denise E. Wall, Steven H. Zeisel

Faculty Publications

Isothiocyanates (ITC) are potentially anticarcinogenic phytochemicals formed from the metabolism of glucosinolates and are found in cruciferous vegetables as well as a select number of other foods. ITC are both substrates for and inducers of glutathione S-transferase (GST) phase II metabolizing enzymes involved in carcinogen detoxification as well as effectors of phase I pathways. Previous studies report mixed results on the interaction between cruciferous vegetable intake, GST polymorphisms, and risk of cancer. We conducted a study of 114 healthy human subjects between 18 and 50 y of age to examine the biologic mechanism underlying the associations, specifically, to assess …

The Administrative Judiciary's Independence Myth, James E. Moliterno

Faculty Publications

No abstract provided.

Use Of Complementary And Alternative Medicine For Weight Control In The United States, Patricia A. Sharpe, Heidi M. Blanck, Joel E. Williams, Barbara E. Ainsworth, Joan M. Conway

Faculty Publications

Objectives: The purpose was to assess the prevalence and correlates of complementary and alternative medicine use for weight control.

Design: A list-assisted random-digit-dialed telephone survey of adults was conducted in the fall of 2002 (n = 11,211). The focus of the study was complementary and alternative medicine (CAM) use, other than dietary supplements, in the previous 12 months.

Settings/location: The sample of respondents was drawn from the total noninstitutionalized U.S. adult population residing in telephone-equipped locations.

Subjects: The sampling procedures were designed to obtain adequate representation of Hispanic and non-Hispanic black respondents. Data from the total sample of 11,211 …

Landscape Context Outweighs Local Habitat Quality In Its Effects On Herbivore Dispersal And Distribution, Kyle J. Haynes, Forrest P. Dillemuth, Bryan J. Anderson, Alyssa S. Hakes, Heather B. Jackson, S. Elizabeth Jackson, James T. Cronin

Faculty Publications

Past studies with spatially structured herbivore populations have emphasized the primacy of intrinsic factors (e.g., patch quality), patch geometry (e.g., patch size and isolation), and more recently landscape context (e.g., matrix composition) in affecting local population abundance and dispersal rate. However, few studies have examined the relative importance of each factor, or how they might interact to affect herbivore abundance or dispersal. Here, we performed a factorial field experiment to examine the independent and interactive effects of patch quality (plant biomass, leaf protein, leaf phenolics) and matrix composition [mudflat or non-host grass (Bromus inermis)] on planthopper (Prokelisia crocea) emigration from …

Production Of F1 Interspecies Hybrid Offspring With Cryopreserved Sperm From A Live-Bearing Fish, The Swordtail Xiphophorus Helleri, Huiping Yang, Leona Hazlewood, Sheila J. Heater, Paula A. Guerrero, Ronald B. Walter, Terrence R. Tiersch

Faculty Publications

Despite study of sperm cryopreservation in more than 200 fish species, production of broods from cryopreserved sperm in live-bearing fish has not been demonstrated. This has not been due to a lack of effort, but instead is a result of the unique morphology, biology, and biochemistry of reproduction in viviparous fishes. For example, sperm of Xiphophorus helleri have a cylindrical nucleus, can swim for days after being activated, have glycolytic capabilities, and can reside in the female reproduction tract for months before fertilization. These traits are not found in fishes with external fertilization. The long-standing research use of the genus …

Grass Shrimp (Palaemonetes Spp.) Play A Pivotal Trophic Role In Enhancing Ruppia Maritima, Donna Drury Mccall, Chet R. Rakocinski

Faculty Publications

Coupled trophic-engineer interactions are potentially important for maintaining habitat function and ecosystem services. As ephemeral submerged aquatic vegetation (SAV), Ruppia maritima has a short well-defined growth-senescence cycle and should benefit from any ecological interaction that enhances its physical condition and longevity. Grass shrimp (Palaemonetes spp.) are abundant facultative grazers of epiphytic algae and conveyors of nutrients in tidal marsh and SAV habitats. Grass shrimp addition consistently enhanced Ruppia biomass and shoot density in a series of three field experiments conducted in Grand Bay National Estuarine Research Reserve, Mississippi, USA. In two experiments, epiphyte grazing by grass shrimp enhanced Ruppia by …

Objectively Measured Physical Activity And Fat Mass In A Large Cohort Of Children, Andy R. Ness, Sam D. Leary, Calum Mattocks, Steven N. Blair, John J. Reilly, Jonathan Wells, Sue Ingle, Kate Tilling, George Davey Smith, Chris J. Riddoch

Faculty Publications

Background: Previous studies have been unable to characterise the association between physical activity and obesity, possibly because most relied on inaccurate measures of physical activity and obesity.

Methods and Findings: We carried out a cross sectional analysis on 5,500 12-year-old children enrolled in the Avon Longitudinal Study of Parents and Children. Total physical activity and minutes of moderate and vigorous physical activity (MVPA) were measured using the Actigraph accelerometer. Fat mass and obesity (defined as the top decile of fat mass) were measured using the Lunar Prodigy dual x-ray emission absorptiometry scanner. We found strong negative associations between MVPA and …

Seaweed And Soy: Companion Foods In Asian Cuisine And Their Effects On Thyroid Function In American Women, Jane Teas, Lewis E. Braverman, Mindy S. Kurzer, Sam Pino, Thomas G. Hurley, James R. Hébert

Faculty Publications

Seaweeds and soy are two commonly eaten foods in Asia. Both have been reported to affect thyroid function, seaweed because of its iodine content and soy because of its goitrogenic effect. Twenty-five healthy postmenopausal women (mean age 58 years) completed a double-blinded randomized crossover study. Ten capsules (5 g/day) of placebo or seaweed (Alaria exculenta), providing 475 ug of iodine/day, were consumed daily for 7 weeks. A powdered soy protein isolate (Solae Co., St. Louis, MO) providing 2 mg of isoflavones/kg of body weight, was given daily during the last week of each treatment arm. On average, …

Timescale Dependence Of Environmental And Plant‐Mediated Controls On Ch4 Flux In A Temperate Fen, Claire C. Treat, Jill L. Bubier, Ruth K. Varner, Patrick M. Crill

Faculty Publications

This study examined daily, seasonal, and interannual variations in CH4 emissions at a temperate peatland over a 5‐year period. We measured net ecosystem CO2 exchange (NEE), CH4 flux, water table depth, peat temperature, and meteorological parameters weekly from the summers (1 May to 31 August) of 2000 through 2004 at Sallie's Fen in southeastern New Hampshire, United States. Significant interannual differences, driven by high variability of large individual CH4 fluxes (ranging from 8.7 to 3833.1 mg CH4 m−2 d−1) occurring in the late summer, corresponded with a decline in water table level and an increase in air and peat temperature. …

Validation Of The Physical Activity Questionnaire For Older Children In Children Of Different Races, Justin B. Moore, John C. Hanes Jr., Paule Barbeau, Bernard Gutin, Roberto P. Treviño, Zenong Yin

Faculty Publications

The Physical Activity Questionnaire for Older Children (PAQ-C) is a validated self-report measure of physical activity widely used to assess physical activity in children (8-14 years of age). To date, however, the instrument has been validated in largely White Canadian samples. The purpose of the present article is to determine the pscyhometric properties of the PAQ-C for African American, European American, and Hispanic children. Two studies were conducted in which independent samples were administered the PAQ-C, along with varying indices of cardiovascular fitness, fatness, and psychological measures related to physical activity. Results showed that the reliability and validity of the …

A Dairy-Based Espresso Beverage Manufactured Using Three Different Coffee Bean Roasts, Charles A. Boeneke, John U. Mcgregor, Kayanush J. Aryana

Faculty Publications

The objective of this study was to evaluate the effects of coffee bean roast on the acceptability of a dairy-based espresso drink. Espresso coffee was made from freshly ground coffee beans with light-medium roast, between roast (between medium and dark) and dark French roast in order to show the influence of roasting on flavor. Samples were prepared by blending skim milk, soft-serve ice cream mix, ice and fresh espresso extracts. No significant differences (P < 0.05) were detected in total solids and fat content among treatments. Viscosity for the light-medium roasted beverage was significantly (P < 0.05) higher than the others. The beverage pH increased with increased darkening of roast. The beverage made with the light-medium roasted coffee had significantly (P < 0.05) higher preference scores for coffee flavor and overall flavor than beverages made with the between and French roasts. The between and French roasted treatments were shown to have a more intense coffee flavor by trained sensory panelists. Consumers in this study preferred a less intense coffee flavor. © 2007, Blackwell Publishing.

Food Insecurity And Subsequent Weight Gain In Women, Sonya J. Jones, Edward A. Frongillo Jr.

Faculty Publications

Objective: Cross-sectional data indicate that a relationship between household food insecurity and overweight exists among women in the USA. Cross-sectional data cannot determine if food insecurity leads to overweight as some have hypothesised. The purpose of the present study was to examine the relationship of food insecurity with subsequent weight gain in women using data from the Panel Study of Income Dynamics (PSID).

Design, setting and subjects:Panel data from the 1999 and 2001 PSID, a nationally representative sample of households, were analysed using multivariate regression procedures.

Results: Average weight gain among all women (n=5595) was 1.1 kg …

Model-Based Compartmental Analysis Indicates A Reduced Mobilization Of Hepatic Vitamin A During Inflammation In Rats, Sin Gieng, Michael Green, Joanne Green, Francisco Rosales

Faculty Publications

Vitamin A (VA) kinetics was studied in rats with marginal VA stores before, during, and after inflammation. Rats received orally [11,12-3H(N)]retinol ([3H]VA; day 0), and inflammation was induced on day 21 with lipopolysacchride (LPS) for 3 days (n = 5) or recombinant human interleukin-6 (rhIL-6) for 7 days (n = 5). Both the fraction of [3H]VA and retinol concentrations in plasma were reduced significantly by LPS or rhIL-6. Compartmental analysis using the Windows version of Simulation, Analysis, and Modeling software was applied to group mean data, and non-steady-state models were developed. After absorption, VA kinetics was described by a three-compartment …

Enzymes As Feed Additive To Aid In Responses Against Eimeria Species In Coccidia-Vaccinated Broilers Fed Corn-Soybean Meal Diets With Different Protein Levels, J. Parker, E. O. Oviedo Rondon, Beatrice A. Clack, S. Clemente-Hernandez, J. Osborne, J. C. Remus, H. Kettunen, H. Makivuokko, E. M. Pierson

Faculty Publications

This research aimed to evaluate the effects of adding a combination of exogenous enzymes to starter diets varying in protein content and fed to broilers vaccinated at day of hatch with live oocysts and then challenged with mixed Eimeria spp. Five hundred four 1-d-old male Cobb-500 chickens were distributed in 72 cages. The design consisted of 12 treatments. Three anticoccidial control programs [ionophore (IO), coccidian vaccine (COV), and coccidia-vaccine + enzymes (COV + EC)] were evaluated under 3 CP levels (19, 21, and 23%), and 3 unmedicated-uninfected (UU) negative controls were included for each one of the protein levels. All …

Assessment Of Genetic Diversity In The Usda And Cip-Fao International Nursery Collections Of Quinoa (Chenopodium Quinoa Willd.) Using Microsatellite Markers [Abstract], S. A. Christensen, Donald B. Pratt, C. Pratt, P. T. Nelson, M. R. Stevens, Eric N. Jellen, C. E. Coleman, D. J. Fairbanks, A. Bonifacio, Peter J. Maughan

Faculty Publications

No abstract provided.

Red-Cockaded Woodpecker Foraging Behavior, D. Craig Rudolph, Richard N. Conner, Richard R. Schaefer, Nancy E. Koerth

Faculty Publications

We studied Red-cockaded Woodpeckers (Picoides borealis) to examine the effect of status and gender on foraging behavior. Foraging behavior of breeding pairs extended beyond separation by foraging height to include zones (bole, trunk in crown, primary limb, secondary limb) of the tree used and foraging methods (scaling, probing, excavating). Helper males and juvenile females maintained partial spatial separation from breeding adults. Helper males maintained spatial separation from breeding adults by exploiting limbs within tree crowns in both longleaf (Pinus palustris) and loblolly-shortleaf (P. taeda, P. echinata) pine forests, but also increased use of boles in loblolly-shortleaf pine in concert with …

Sex And Seasonal Co-Variation Of Arginine Vasotocin (Avt) And Gonadotropin-Releasing Hormone (Gnrh) Neurons In The Brain Of The Halfspotted Goby, Karen P. Maruska, Mindy H. Mizobe, Timothy C. Tricas

Faculty Publications

Gonadotropin-releasing hormone (GnRH) and arginine vasotocin (AVT) are critical regulators of reproductive behaviors that exhibit tremendous plasticity, but co-variation in discrete GnRH and AVT neuron populations among sex and season are only partially described in fishes. We used immunocytochemistry to examine sexual and temporal variations in neuron number and size in three GnRH and AVT cell groups in relation to reproductive activities in the halfspotted goby (Asterropteryx semipunctata). GnRH-immunoreactive (-ir) somata occur in the terminal nerve, preoptic area, and midbrain tegmentum, and AVT-ir somata within parvocellular, magnocellular, and gigantocellular regions of the preoptic area. Sex differences were found among all …

Laboratory Evaluation Of Diflubenzuron As A Feed-Through For Control Of Immature Sand Flies (Diptera: Psychodidae), T. M. Mascari, M. A. Mitchell, E. D. Rowton, L. D. Foil

Faculty Publications

The benzoylurea chitin synthesis inhibitor diflubenzuron was evaluated as a rodent feed-through for the control of immature stages of Phlebotomus papatasi Scopoli (Diptera: Psychodidae). The development and survival of second instars of P. papatasi larvae that were fed feces from Syrian hamsters, Mesocricetus auratus, that had been fed a diet containing 0, 8.97, 89.7, or 897 ppm diflubenzuron was evaluated. No pupation or adult emergence occurred when larvae were fed feces from hamsters that were fed diets containing diflubenzuron. The mortality of sand flies fed feces from treated hamsters was coincident with pupation of the controls, suggesting a specific effect …

The Role Of The Specific, Profilaggrin-Containing Keratohyalin Granules In The Developing Epidermis Of The Fetal Horse Hoof, Hermann H. Bragulla, Dominique G. Homberger

Faculty Publications

The adult equine hoof is subdivided into distinct segments with various keratinization modes. In the periople and bulbs of the heel, the epidermis forms a Stratum granulosum with basophilic keratohyalin granules during soft keratinization, whereas in the coronet, wall proper, sole, and frog, the epidermis undergoes hard keratinization by keratinizing and comifying without forming keratohyalin granules. The present study tests the hypothesis that the presence of specific (profilaggrin-containing) keratohyalin granules in the hoof epidermis is correlated with the water-binding capacity and mechanical properties of the hoof horn. To identify these specific profilaggrin-containing keratohyalin granules, tissue samples of fetal hooves were …

Common Single-Nucleotide Polymorphisms Act In Concert To Affect Plasma Levels Of High-Density Lipoprotein Cholesterol, Victor Spirin, Steffen Schmidt, Alexander Pertsemlidis, Richard S. Cooper, Jonathan C. Cohen, Shamil R. Sunyaev

Faculty Publications

The identification of DNA sequence variants underlying human complex phenotypes remains a significant challenge for several reasons: individual variants can have small phenotypic effects or low population frequencies, and multiple allelic variants may act in concert to affect a trait. We evaluated the combined effect of allelic variants in seven genes involved in high-density lipoprotein (HDL) metabolism, using forward stepwise regression. Analysis of all known common single-nucleotide polymorphisms (SNPs) in the seven candidate genes revealed four variants that were associated with incremental changes in HDL cholesterol levels in three independent samples. Conversely, analysis of 660 polymorphisms in eight genes that …

Biology And Host Specificity Of Gall-Inducing Acythopeus Burkhartorum (Coleoptera: Curculionidae), A Biological-Control Agent For The Invasive Weed Coccinia Grandis (Cucurbitaceae) In Guam And Saipan, Anantanarayanan Raman, Zerlene T. Cruz, Rangaswamy Muniappan, Gadi V.P. Reddy

Faculty Publications

An introduced cucurbit Coccinia grandis (Linnaeus) Voigt has grown into problem proportions in Hawaii and the Pacific islands of Guam and Saipan. The biology of Acythopeus burkhartorum OBrien, 1998, a potential biological-control agent of C. grandis, has been described. By inducing the gall a sink for nutrients and by deriving nutrition, A. burkhartorum places C. grandis under stress. Especially during the late larval stage, the weevil displays an unusual behaviour of shredding dry gall tissues with its mandibles to prepare the pupal case and using the shredded sclerenchyma fibres to fill the open and cut ends of the pupal case. …

Benefits Of Urea-Molasses Block Supplementation And Symptomatic And Tactical Anthelmintic Treatments Of Communally Grazed Indigenous Goats In The Bulwer Area, Kwazulu-Natal Province, South Africa, A. F. Vatta, J. F. De Villier, S. A. Gumede, R. C. Krecek, N. P. Mapeyi, R. A. Pearson, M. F. Smith, M. O. Stenson, L. J.S. Harrison

Faculty Publications

This study was carried out with the cooperation of farmers owning communally grazed indigenous goats in southwestern KwaZulu-Natal Province, South Africa, where farmers had identified poor reproductive performance in their herds as one of their major problems. The aim was to quantify the effects of 3 interventions and the interaction between these interventions on goat productivity and gastrointestinal nematode infection. The interventions were: urea-molasses block supplementation during the dry winter seasons of 2004 and 2005, tactical anthelmintic treatment with ivermectin (400 μg/kg) during the wet summer period (on 3 January 2005) and symptomatic treatment with ivermectin (400 μg/kg) of all …

Antioxidant Activity And Phenolic Composition In 'Beauregard' Sweetpotato Are Affected By Root Size And Leaf Age, Malkeet S. Padda, D. H. Picha

Faculty Publications

Phenolic acids are considered important antioxidants that may help to prevent many human chronic diseases. The antioxidant activity and phenolic content of sweetpotato [Ipomoea batatas (L.) Lam.] roots and leaves of different sizes and ages, respectively, were quantified. Small roots (≈4 g root weight) had a higher antioxidant activity and phenolic content compared with full-sized marketable roots (≈300 g root weight). Phenolic content in marketable roots was significantly higher in the cortex tissue than in the internal pith tissue. The highest total phenolic content [chlorogenic acid equivalents (10.3 mg·g-1 dry weight)] and antioxidant activity [Trolox equivalents (9.7 mg·g-1 dry weight)] …

The Debtor-Patient Revisited, Melissa B. Jacoby

Faculty Publications

No abstract provided.

Change In Physical Activity Participation Among Adolescent Girls From 8th To 12th Grade, Russell R. Pate, Marsha Dowda, Jennifer R. O'Neill, Dianne S. Ward

Faculty Publications

Background: Physical activity levels of girls decline in adolescence, but little is known about changes in participation in specific types of physical activity. This study examined change in participation in specific activities during adolescence in girls. Methods: Girls (N=398, age 13.6 ± 0.6 y at baseline, 58.5% African American) from 31 middle and 24 high schools in South Carolina completed the 3-Day Physical Activity Recall (3DPAR) in 8th, 9th, and 12th grades. Girls reported their predominant activity and its intensity level in each 30-min time block on the previous 3 d. Results: Vigorous physical activity declined from 45.4% …

Characteristics Of Reduced Fat Milks As Influenced By The Incorporation Of Folic Acid, K. Achanta, C. A. Boeneke, K. J. Aryana

Faculty Publications

Folic acid plays an important role in the prevention of neural tube defects (e.g., spina bifida and anencephaly), heart defects, facial clefts, urinary abnormalities, and limb deficiencies. Milk and milk products serve as a potential source for folic acid fortification because of the presence of folate-binding proteins that seem to be involved in folate bioavailability. Although milk is not a good source of folic acid, fortification could help in the prevention of the above-mentioned defects. The objective of this study was to examine the physicochemical characteristics of reduced fat milks fortified with folic acid. Reduced fat milks were prepared using …

Genetic Diversity And Low Reproductive Success In Isolated Populations Of Utah Juniper (Juniperus Osteosperma, Cupressaceae), Loreen Allphin, Val J. Anderson, Amy F. Hunt

Faculty Publications

Utah juniper (Juniperus osteosperma) has greatly expanded its historical range in the western United States. Management plans for the species have focused on curtailing its encroachment into sagebrush and grassland communities. These plans often include burning or other methods of elimination. These methods may result in subdivision or fragmentation of existing juniper stands. We initiated a study at Dugway Proving Ground, a U.S. Army facility, to examine the effects of fragmentation on the reproductive success of Utah juniper in isolated populations. We used enzyme electrophoresis to quantify genetic variability in isolated populations. We also determined population reproductive success by examining …

Giving Birth: The Voices Of Russian Women, Lynn Clark Callister, Natalia Getmanenko, Natalia Garvrish, Marakova Olga Eugenevna, Zotina Nataliya Vladimirova, Jane H. Lassetter, Natalia Turkina

Faculty Publications

Purpose: To gain an understanding of the meaning of childbirth to women living in St. Petersburg, the Russian Federation.

Study Design and Method: Twenty-four Russian women who had given birth in the past 6 months participated in this phenomenological study and were interviewed and audio-taped about the meaning of childbirth. Data collection and analysis occurred concurrently.

Results: The Russian sociocultural context of childbirth was identified in specific themes: (1) adhering to cultural traditions, (2) accessing information, (3) maintaining a healthy lifestyle, (4) articulating the spiritual and emotional meaning of giving birth, and (5) transforming as a mother and a person. …