Digital Commons Network^™

Long-Term Effects Of A Physical Activity Intervention In High School Girls, Russell R. Pate, Ruth P. Saunders, Rod K. Dishman, Cheryl L. Addy, Marsha Dowda, Dianne S. Ward

Faculty Publications

Background - Physical activity decreases during childhood and adolescence, and physical activity levels are significantly lower in females than males, particularly during adolescence. Schools are attractive settings in which to implement interventions designed to promote physical activity in girls and young women, but few studies have tested the sustained effects of such interventions.

Design - Cross-sectional. Data were collected in 2002-2003 and analyzed in 2006-2007.

Setting/Participants - 1594 adolescent girls in 22 high schools.

Intervention - The intervention, Lifestyle Education for Activity Program (LEAP), was designed to increase physical activity in 9^th grade girls through two channels: changes in …

Toward A Population Health Model Of Segmented Assimilation: The Case Of Low Birth Weight In Los Angeles, Brian K. Finch, Nelson Lim, William Perez, D. Phuong Do

Faculty Publications

The authors adapt the segmented assimilation theory to a model population health, which posits that assimilation is actually harmful to migrants' health. The authors also specify models of individual and contextual factors to indirectly test the theory of segmented assimilation - a theory that posits interactions between individual and residential circumstances. Using Year 2000 vital statistics data merged with 2000 U.S. census data from Los Angeles County, the authors model the probability of being born low birth weight among the native and foreign born. Results confirm an immigrant advantage at the individual level and protective effects of immigrant coresidence at …

Byu Adds "Zing" To New Carbonated Yogurt Product In Stores Nationwide, Byu News

Faculty Publications

A BYU professor invented the "zing" in General Mills' new carbonated yogurt product, now available in stores all over the country. Fizzix(TM), an extension of Yoplait's Go-GURT brand that comes in a tube, adds a tangy tingle to the traditional fruity flavor of yogurt that will "fizzify" the tongues of the 8- to 12-year-olds the company is targeting.

Effect Of An Herbal Extract Number Ten (Nt) On Body Weight In Rats, David A. York, Sonyja Thomas, Frank L. Greenway, Zhijun Liu, Jennifer C. Rood

Faculty Publications

Background: Chinese herbal extract Number Ten (NT) is a dietary herbal formulation prepared from rhubarb, ginger, astragalus, red sage and tumeric. This study tested the effectiveness of NT in reducing body weight gain in rats. Methods: Sixty female Wistar rats were fed a high fat diet and acclimated to gavage feeding. The rats were divided into five treatment groups: (1) Control (n = 15); (2) NT-H (n = 15), 1.5 g/day; (3) NT-L (n = 10), 0.75 g/day; (4) Pr-fed (n = 10), pair fed to NT-H; (5) d-FF (n = 10), d-fenfluramine 2 mg/kg. Ten rats per group were …

Comparative Microbiota Of Rickettsia Felis-Uninfected And -Infected Colonized Cat Fleas, Ctenocephalides Felis, Walairat Pornwiroon, Michael T. Kearney, Claudia Husseneder, Lane D. Foil, Kevin R. Macaluso

Faculty Publications

Fleas serve as arthropod vectors for several emerging and re-emerging infectious disease causing agents including, Rickettsia felis. Although the prevalence of R. felis infection in colonies of fleas has been examined, the influence of the R. felis infection on flea microbiota has not been investigated. We identified three colonies of cat fleas, Ctenocephalides felis, with varying prevalence of R. felis infection (Louisiana State University (LSU), 93.8; Professional Laboratory and Research Services Inc. (PLRS), 16.4; Elward II (EL), 0) and subsequently utilized polymerase chain reaction amplification, restriction fragment length polymorphism analysis and sequencing of the 1.4-kb portions of 16S rRNA genes …

Description, Life History, And Parasitism Of A New Species Of Delphacid Planthopper (Hemiptera: Fulgoroidea), James T. Cronin, Stephen W. Wilson

Faculty Publications

Delphacodes scolochloa Cronin & Wilson (Hemiptera: Fulgoroidea) is a newly discovered delphacid planthopper that feeds and oviposits exclusively on the stems of sprangletop, Scolochloa festucacea (Willd.) Link (Poaceae: Pooidae), in the prairie pothole region of northeastern North Dakota. D. scolochloa is bivoltine. It is also wing dimorphic, but populations are composed predominantly of flightless brachypters. Macropters incur a substantial cost in terms of reduced longevity and fecundity relative to brachypters. The parasitoid complex attacking D. scolochloa consists of two egg parasitoids, Anagrus nigriventris Girault and Anagrus columbi Perkins (Hymenoptera: Mymaridae), and one undescribed dryinid. The proportion of eggs parasitized averages …

Fungi Isolated From Integument And Guts Of Coptotermes Formosanus And Their Antagonistic Effect On Gleophyllum Trabeum, Poornima Jayasimha, Gregg Henderson

Faculty Publications

In our earlier efforts to demonstrate the spread of a brown rot fungus, Gloeophyllum trabeum (Pers.) Murrill by Formosan subterranean termites, Coptotermes formosanus Shiraki, we found not only that C. formosanus does not spread G. trabeum but also that G. trabeum did not survive in the presence of C. formosanus. Further investigation of this antagonistic interaction between this termite and fungus led to a hypothesis that green-spored fungi may be carried by termites and that they play a role in suppressing the growth of G. trabeum. Fungal cultures were isolated from integument and guts of laboratory-maintained colonies of C. formosanus …

Classifying Foods In Contexts: How Adults Categorize Foods For Different Eating Settings, Christine E. Blake, Carole A. Bisogni, Jeffrey Sobal, Carol M. Devine, Margaret M. Jastran

Faculty Publications

This project examined adults' food cognitions by applying schema theory to explain how adults categorized foods for different contexts. Qualitative interviews and repeated card sort activities for different eating contexts were conducted to elicit as many food categories as possible form 42 US adults. Participants labeled card sort piles with their own words, providing 991 card sort labels. Qualitative analysis of the labels resulted in the emergence of 12 category types. Personal-experience-based types were specific to the individual (e.g., Preference). Context-based types were related to situational aspects of eating episodes (e.g., Location). Food-based types were related to intrinsic properties of …

Algal Regulation Of Extracellular Enzyme Activity In Stream Microbial Communities Associated With Inert Substrata And Detritus, Steven T. Rier, Kevin A. Kuehn, Steven N. Francoeur

Faculty Publications

We tested the hypothesis that algae influence the activities of extracellular enzymes involved in mineralization processes within microbial assemblages in streams. We tested the prediction that the factors that influence algal biomass and photosynthesis (i.e., diel fluctuations in photosynthetically active radiation [PAR], long-term variations in light regime, and community development stage) would have a corresponding effect on extracellular enzyme activities. We also tested the prediction that algae would influence enzyme activities on inorganic substrata and in detrital communities where they ultimately would influence plant litter decomposition rates. We allowed microbial communities to develop on inert substrata (glass-fiber filters) or on …

Physical Activity And Public Health: Updated Recommendation For Adults From The American College Of Sports Medicine And The American Heart Association, William L. Haskell, I-Min Lee, Russell R. Pate, Kenneth E. Powell, Steven N. Blair, Barry A. Franklin, Caroline A. Macera, Gregory W. Heath, Paul D. Thompson, Adrian Bauman

Faculty Publications

Summary - In 1995 the American College of Sports Medicine and the Centers for Disease Control and Prevention published national guidelines on Physical Activity and Public Health. The Committee on Exercise and Cardiac Rehabilitation of the American Heart Association endorsed and supported these recommendations. The purpose of the present report is to update and clarify the 1995 recommendations on the types and amounts of physical activity needed by healthy adults to improve and maintain health. Development of this document was by and expert panel of scientists, including physicians, epidemiologists, exercise scientists, and public health specialists. This panel reviewed advances in …

Physical Activity And Public Health In Older Adults: Recommendation From The American College Of Sports Medicine And The American Heart Association, Miriam E. Nelson, W. Jack Rejeski, Steven N. Blair, Pamela W. Duncan, James O. Judge, Abby C. King, Carol A. Macera, Carmen Castaneda-Sceppa

Faculty Publications

Objective - To issue a recommendation on the types and amounts of physical activity needed to improve and maintain health in older adults.

Participants - A panel of scientists with expertise in public health, behavioral science, epidemiology, exercise science, medicine, and gerontology.

Evidence - The expert panel reviewed existing consensus statements and relevant evidence from primary research articles and reviews of the literature. Process: After drafting a recommendation for the older adult population and reviewing drafts of the Updated Recommendation from the American College of Sports Medicine (ACSM) and the American Heart Association (AHA) for Adults, the panel issued a …

Functional Analysis Of Polyphenol Oxidases By Antisense/Sense Technology, Piyada Thipyapong, Michael J. Stout, Jutharat Attajarusit

Faculty Publications

Polyphenol oxidases (PPOs) catalyze the oxidation of phenolics to quinones, the secondary reactions of which lead to oxidative browning and postharvest losses of many fruits and vegetables. PPOs are ubiquitous in angiosperms, are inducible by both biotic and abiotic stresses, and have been implicated in several physiological processes including plant defense against pathogens and insects, the Mehler reaction, photoreduction of molecular oxygen by PSI, regulation of plastidic oxygen levels, aurone biosynthesis and the phenylpropanoid pathway. Here we review experiments in which the roles of PPO in disease and insect resistance as well as in the Mehler reaction were investigated using …

Seed Preferences Of Wintering Henslow's Sparrows, Jennifer K. Dimiceli, Philip C. Stouffer, Erik I. Johnson, Claudia Leonardi, Edgar B. Moser

Faculty Publications

The Henslow's Sparrow (Ammodramus henslowii), a species of conservation concern, winters primarily in longleaf pine (Pinus palustris) forest ecosystems in the southeastern U.S. These pine savannas have been reduced to 5% of their former range, with remaining patches requiring active management with fire to maintain characteristic structure and plant diversity. Wintering Henslow's Sparrow abundance tracks growing-season fires; bird abundance peaks in the winter following burning, then declines in subsequent winters. Fire also determines dominant plant species, suggesting that Henslow's Sparrows may respond to abundance of preferred seeds. To determine diet preferences of Henslow's Sparrows, we tested seeds from eight species …

Agreement Between Student-Reported And Proxy-Reported Physical Activity Questionnaires, Marsha Dowda, Russell R. Pate, James F. Sallis, Patty S. Freedson, Wendell C. Taylor, John R. Sirard, Stewart G. Trost

Faculty Publications

Parents and 531 students (46% males, 78% white) completed equivalent questionnaires. Agreement between student and parent responses to questions about hypothesized physical activity (PA) correlates was assessed. Relationships between hypothesized correlates and an objective measure of student's moderate-to-vigorous physical activity (MVPA) in a subset of 177 students were also investigated. Agreement between student and parent ranged from r=.34 to .64 for PA correlates. Spearman correlations between MVPA and PA correlates ranged from -.04 to .21 for student report and -.14 to .32 for parent report, and there were no statistical differences for 8 out of 9 correlations between parent …

Assessment Of Genetic Diversity In The Usda And Cip-Fao International Nursery Collections Of Quinoa (Chenopodium Quinoa Willd.) Using Microsatellite Markers, S. A. Christensen, D. B. Pratt, C. Pratt, P. T. Nelson, M. R. Stevens, Eric N. Jellen, Craig E. Coleman, Daniel J. Fairbanks, A. Bonifacio, Michael J. Maughan

Faculty Publications

Quinoa (Chenopodium quinoa Willd.) is a staple food crop for millions of impoverished rural inhabitants of Andean South America where it has been cultivated for millennia. Interest in quinoa, due largely to its superior nutritional characteristics, is fuelling a growing export market and has led to an increased focus on genetic research and the development of quinoa breeding programmes throughout South America. The success of these breeding programmes will rely heavily on the development of core germplasm collections and germplasm conservation. We report the development of a set of fluorescence-tagged microsatellite molecular markers that can be used to characterize genetic …

Weekend And Weekday Patterns Of Physical Activity In Overweight And Normal-Weight Adolescent Girls, Margarita S. Treuth, Diane J. Catellier, Kathryn H. Schmitz, Russell R. Pate, John P. Elder, Robert G. Mcmurray, Robert M. Blew, Song Yang, Larry S. Webber

Faculty Publications

Objective - To describe the patterns (specifically comparing weekdays and weekends classified by intensities) of physical activity (PA) measured by accelerometry in adolescent girls.

Research Methods and Procedures - Healthy sixth grade girls (n=1603), 11 to 12 years old, were randomly recruited from 36 schools participating in the Trial of Activity in Adolescent Girls. Age, ethnicity, socioeconomic status, weight, and height were taken. PA patterns were measured for 6 days using accelerometry.

Results - Adolescent girls spend most of their time in sedentary (52% to 57% of the day) and light activity (40% to 45% of the day) …

Genome-Wide Association Scan Shows Genetic Variants In The Fto Gene Are Associated With Obesity-Related Traits, Angelo Scuteri, Serena Sanna, Wei Min Chen, Manuela Uda, Giuseppe Albai, James Strait, Samer Najjar, Ramaiah Nagaraja, Marco Orrú, Gianluca Usala, Mariano Dei, Sandra Lai, Andrea Maschio, Fabio Busonero, Antonella Mulas, Georg B. Ehret, Ashley A. Fink, Alan B. Weder, Richard S. Cooper, Pilar Galan, Aravinda Chakravarti, David Schlessinger, Antonio Cao, Edward Lakatta, Gonçalo R. Abecasis

Faculty Publications

The obesity epidemic is responsible for a substantial economic burden in developed countries and is a major risk factor for type 2 diabetes and cardiovascular disease. The disease is the result not only of several environmental risk factors, but also of genetic predisposition. To take advantage of recent advances in gene-mapping technology, we executed a genome-wide association scan to identify genetic variants associated with obesity-related quantitative traits in the genetically isolated population of Sardinia. Initial analysis suggested that several SNPs in the FTO and PFKP genes were associated with increased BMI, hip circumference, and weight. Within the FTO gene, rs9930506 …

Suppression Of Growth Of A Brown Rot Fungus, Gloeophyllum Trabeum, By Formosan Subterranean Termites (Isoptera: Rhinotermitidae), Poornima Jayasimha, Gregg Henderson

Faculty Publications

Although a brown rot fungus, Gloeophyllum trabeum (Pers.) Murrill, has been shown to be attractive and beneficial to subterranean termites, including Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), no research has been conducted to determine whether the association is mutualistic. We first investigated whether the fungus could be spread by C. formosanus, because this would represent an obvious benefit to the fungus. We found that Formosan subterranean termites did not spread G. trabeum, rather they suppressed its growth. To further investigate this phenomenon, wood chips were inoculated with G. trabeum, and fungal growth was measured in treatments with termites or without termites …

Mentors And Students Seeking Knowledge, Meredith Jones-Gray

Faculty Publications

No abstract provided.

Neighborhood Context And Ethnicity Differences In Body Mass Index: A Multilevel Analysis Using The Nhanes Iii (1988-1994), D. Phuong Do, Tamara Dubowitz, Chloe E. Bird, Nicole Lurie, José J. Escarce, Brian K. Finch

Faculty Publications

A growing body of literature has documented a link between neighborhood context and health outcomes. However, little is known about the relationship between neighborhood context and body mass index (BMI) or whether the association between neighborhood context and BMI differs by ethnicity. This paper investigates several neighborhood characteristics as potential explanatory factors for the variation of BMI across the United States; further, this paper explores to what extent segregation and the concentration of disadvantage across neighborhoods help explain ethnic disparities in BMI. Using data geo-coded at the census tract-level and linked with individual-level data from the Third National Health and …

Disparities In Mental Health Utilization Among Persons With Chronic Diseases, Saundra H. Glover, Keith T. Elder, Sudha Xirasagar, Jong-Deuk Baek, Crystal N. Piper, Dayna Campbell

Faculty Publications

This study used Aday and Andersen's Behavioral Model of Health Services Use to examine to role of chronic disease and the joint impact of race and chronic disease type on mental health utilization. Using data from Community Tracking Survey Household Survey, we tested the assumption that chronic disease, chronic disease type, and race are related to lower rates of mental health visits when adjusted for predisposing, enabling, and need factors. After adjusting for population characteristics, we found that race significantly moderated the impact of chronic disease type on mental health utilization, showing that African Americans with cardiovascular disease were half …

Quality Of Hermetically Packaged Dehydrated Carrots During Long-Term Storage, S. R. Bartholomew, Laura K. Jefferies, Oscar A. Pike

Faculty Publications

There is interest in the long-term storage of food for applications such as space flight, disaster relief, and personal preparedness. Dehydrated carrots packaged in hermetically sealed cans with a reduced oxygen atmosphere are available in the retail market. The quality of dehydrated carrots stored up to two years has been reported, but the effect of longer-term storage on the quality of the product is unknown. Eleven samples of dehydrated carrots representing 6 brands, packaged in size No.10 cans and stored at room temperature, were obtained from donors. Two fresh samples were purchased as controls. Samples ranged in age from < 1 to 34 years. Can headspace oxygen, can seam integrity, and dehydrated carrot water activity and color were evaluated. Samples were rehydrated for 20 minutes in filtered water that was brought to a boil. A 56-member consumer panel evaluated the rehydrated carrots for appearance, aroma, texture, flavor, and overall acceptability using a 9-point hedonic scale. Acceptance for use in everyday and emergency situations was also determined. Can headspace oxygen ranged from < 0.01% to 14.7%. All can seams were determined to be satisfactory. Water activity of the dehydrated carrots ranged from 0.31 to 0.38. L*, a* and b* values ranged from 37.8 to 44.0, 17.1 to 30.2 and 23.0 to 37.3, respectively. The orange color of most samples faded during storage. Hedonic scores for overall acceptability of dehydrated carrots declined from 7.1 to 3.4. All samples had an acceptance for use in an emergency situation of over 70%. Results indicate that dehydrated carrots decline in quality during long-term storage but retain sufficient sensory acceptance to be considered for use in long-term storage regimens.

Quality Of Hermetically Packaged Split Peas During Long-Term Storage, J. S. Chapman, Laura K. Jefferies, Oscar A. Pike

Faculty Publications

There is a market for low moisture foods that can be stored for long periods of time for use in disaster relief and other emergency situations. Split peas hermetically sealed in cans with a reduced oxygen atmosphere are available in the retail market, but the effect of long-term storage on the quality of the product is unknown. Ten samples of split peas representing 5 retail brands packaged in size No.10 cans and stored at room temperature were obtained from donors. Two fresh samples of split peas were purchased as controls. Samples ranged in age from <1 to 34 years. Can headspace oxygen, can seam integrity, and split pea water activity and color were evaluated. A 52-member consumer panel evaluated the samples, prepared as split pea soup, for appearance, aroma, texture, flavor, and overall acceptability using a 9-point hedonic scale. Acceptance for use in everyday and emergency situations was also determined. Can headspace oxygen ranged from 0.19 to 20.1%. All can seams were determined to be satisfactory. Water activity of the raw split peas ranged from 0.41 to 0.56. The green color of raw split peas decreased over time as shown by increasing CIE a* values. Flavor, appearance, texture, and overall acceptability hedonic scores ranged from 6.7 to 4.4 and decreased over time. Hedonic scores for appearance were correlated with the decrease in raw product green color (r2 = .928). Hedonic scores for texture declined over time, corresponding with increasing hardness of the peas. All samples had an acceptance in an emergency situation of over 75%. Results indicate split pea quality declines over time, but the product maintains sufficient sensory acceptance to be considered for use in applications requiring long-term storage.

Applications Of Chitosan For Improvement Of Quality And Shelf Life Of Foods: A Review, H. K. No, S. P. Meyers, W. Prinyawiwatkul, Z. Xu

Faculty Publications

Chitosan is a modified, natural biopolymer derived by deacetylation of chitin, a major component of the shells of crustacean. Recently, chitosan has received increased attention for its commercial applications in the biomedical, food, and chemical industries. Use of chitosan in food industry is readily seen due to its several distinctive biological activities and functional properties. The antimicrobial activity and film-forming property of chitosan make it a potential source of food preservative or coating material of natural origin. This review focuses on the applications of chitosan for improvement of quality and shelf life of various foods from agriculture, poultry, and seafood …

Brainstem Cholinergic Modulation Of Muscle Tone In Infant Rats, Andrew J. Gall, Amy Poremba, Mark S. Blumberg

Faculty Publications

In week-old rats, lesions of the dorsolateral pontine tegmentum (DLPT) and nucleus pontis oralis (PnO) have opposing effects on nuchal muscle tone. Specifically, pups with DLPT lesions exhibit prolonged bouts of nuchal muscle atonia (indicative of sleep) and pups with PnO lesions exhibit prolonged bouts of high nuchal muscle tone (indicative of wakefulness). Here we test the hypothesis that nuchal muscle tone is modulated, at least in part, by cholinergically mediated interactions between these two regions. First, in unanesthetized pups, we found that chemical infusion of the cholinergic agonist carbachol (22 mM, 0.1 µL) within the DLPT produced high muscle …

A Direct Role Of Jh In The Control Of Imaginal Disc Formation And Growth In Manduca, J. W. Truman, K. Hiruma, J. P. Allee, S.G.B. Macwhinnie, David Champlin Ph.D., L. M. Riddiford

Faculty Publications

No abstract provided.

Exercise And Cardiovascular Events - Placing The Risks Into Perspective: A Scientific Statement From The American Heart Association Council On Nutrition, Physical Activity, And Metabolism And The Council On Clinical Cardiology, Paul D. Thompson, Barry A. Franklin, Gary J. Balady, Steven N. Blair, Domenico Corrado, N. A. Mark Estes Iii, Janet E. Fulton, Neil F. Gordon, William L. Haskell, Mark S. Link, Barry J. Maron, Murray A. Mittleman, Antonio Pelliccia, Nanette K. Wenger, Stefan N. Willich, Fernando Costa

Faculty Publications

Habitual physical activity reduces coronary heart disease events, but vigorous activity can also acutely and transiently increase the risk of sudden cardiac death and acute myocardial infarction in susceptible persons. This scientific statement discusses the potential cardiovascular complications of exercise, their pathological substrate, and their incidence and suggests strategies to reduce these complications. Exercise-associated acute cardiac events generally occur in individuals with structural cardiac disease. Hereditary or congenital cardiovascular abnormalities are predominantly responsible for cardiac events among young individuals, whereas atherosclerotic disease is primarily responsible for these events in adults. The absolute rate of exercise-related sudden cardiac death varies with …

Haplotype Association Between Haptoglobin (Hp2) And Hp Promoter Snp (A-61c) May Explain Previous Controversy Of Haptoglobin And Malaria Protection, Sharon E. Cox, Conor Doherty, Sarah H. Atkinson, Chidi V. Nweneka, Anthony J.C. Fulford, Hala Ghattas Ph.D., Kirk A. Rockett, Dominic P. Kwiatkowski, Andrew M. Prentice

Faculty Publications

Background.

Malaria is one of the strongest recent selective pressures on the human genome, as evidenced by the high levels of varying haemoglobinopathies in human populations-despite the increased risk of mortality in the homozygous states. Previously, functional polymorphisms of Hp, coded by the co-dominant alleles Hp1 and Hp2, have been variously associated with several infectious diseases, including malaria susceptibility.

Methodology/Principal Findings.

Risk of a clinical malarial episode over the course of a malarial transmission season was assessed using active surveillance in a cohort of Gambian children aged 10-72 months. We report for the first time that the major haplotype for …

Haplotype Association Between Haptoglobin (Hp2) And Hp Promoter Snp (A-61c) May Explain Previous Controversy Of Haptoglobin And Malaria Protection, Sharon E. Cox, Conor Doherty, Sarah H. Atkinson, Chidi V. Nweneka, Anthony J.C. Fulford, Hala Ghattas Ph.D., Kirk A. Rockett, Dominic P. Kwiatkowski, Andrew M. Prentice

Faculty Publications

Background

Malaria is one of the strongest recent selective pressures on the human genome, as evidenced by the high levels of varying haemoglobinopathies in human populations–despite the increased risk of mortality in the homozygous states. Previously, functional polymorphisms of Hp, coded by the co-dominant alleles Hp1 and Hp2, have been variously associated with several infectious diseases, including malaria susceptibility.

Methodology/Principal Findings

Risk of a clinical malarial episode over the course of a malarial transmission season was assessed using active surveillance in a cohort of Gambian children aged 10–72 months. We report for the first time that the major haplotype for …

Inhibitory Effects Of Estrogen Receptor Beta On Specific Hormone-Responsive Gene Expression And Association With Disease Outcome In Primary Breast Cancer, Chin-Yo Lin, Anders Strom, Li Say Kong, Silke Kietz, Jane S. Thomsen, Jason B. S. Tee, Vinsensius B. Vega, Lance D. Miller, Johanna Smeds, Jonas Bergh, Jan-Ake Gustafsson, Edison T. Liu

Faculty Publications

The impact of interactions between the two estrogen receptor (ER) subtypes, ER(alpha) and ER(beta), on gene expression in breast cancer biology is not clear. The goal of this study was to examine transcriptomic alterations in cancer cells co-expressing both receptors and the association of gene expression signatures with disease outcome. Methods: Transcriptional effects of ER(beta) overexpression were determined in a stably transfected cell line derived from ER(alpha) -positive T-47D cells. Microarray analysis was carried out to identify differential gene expression in the cell line, and expression of key genes was validated by quantitative polymerase chain reaction. Microarray and clinical data …