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Devolution Of Implementing Policymaking In Network Governments, Charles H. Koch Jr. Jan 2007

Devolution Of Implementing Policymaking In Network Governments, Charles H. Koch Jr.

Faculty Publications

No abstract provided.


A Return To Descartes: Property, Profit, And The Corporate Ownership Of Animals, Darian M. Ibrahim Jan 2007

A Return To Descartes: Property, Profit, And The Corporate Ownership Of Animals, Darian M. Ibrahim

Faculty Publications

No abstract provided.


Solar Dryer Construction And Use Manual Version 2.0 2007, Jonathan P. Rowe, Jonathan K. Russon, Frost M. Steele Jan 2007

Solar Dryer Construction And Use Manual Version 2.0 2007, Jonathan P. Rowe, Jonathan K. Russon, Frost M. Steele

Faculty Publications

In less time than it took to develop this solar dryer and manual, the Pacific Island area has experienced several typhoons and tropical depressions followed by significant flooding and destruction of homes, properties and crops. It is in the interest of all people to become more self-reliant in times of need and to have the basic necessities available at a moment’s notice to sustain life until transportation, commerce, health care, agriculture, water, etc. can be restored to a more normal state. This work was done to enable the people of the Pacific islands to meet some of their food requirements …


Establishment Of Cecidochares Connexa (Diptera: Tephritidae) In Guam And Its Effect On The Growth Of Chromolaena Odorata (Asteraceae), Z. T. Cruz, R. Muniappan, G. V.P. Reddy Nov 2006

Establishment Of Cecidochares Connexa (Diptera: Tephritidae) In Guam And Its Effect On The Growth Of Chromolaena Odorata (Asteraceae), Z. T. Cruz, R. Muniappan, G. V.P. Reddy

Faculty Publications

The gall fly Cecidochares connexa (Macquart) (Diptera: Tephritidae) was imported from Indone ia into Guam in 1998. It was field-established at one location in north central Guam, and its spatial and temporal spread in Guam was monitored. The biology of the gall fly is presented. No relationship between the number of larvae in a gall and the dimensions of the gall (length and width) existed. Three pupae per gall was the most frequent density with a range of 1-7. The sex ratio of flies emerging from galls was 1:1 irrespective of number of larvae per gall. C. connexa-induced galls established …


Rules For Radicals: A Politics Of Patent Law, Kali Murray Oct 2006

Rules For Radicals: A Politics Of Patent Law, Kali Murray

Faculty Publications

No abstract provided.


Physical Activities In Adolescent Girls: Variability In Energy Expenditure, Karin A. Pfeiffer, Kathryn H. Schmitz, Robert G. Mcmurray, Margarita S. Treuth, David M. Murray, Russell R. Pate Oct 2006

Physical Activities In Adolescent Girls: Variability In Energy Expenditure, Karin A. Pfeiffer, Kathryn H. Schmitz, Robert G. Mcmurray, Margarita S. Treuth, David M. Murray, Russell R. Pate

Faculty Publications

Background - Understanding interindividual variability of energy expended in common activities is important for determining precise estimates of energy expenditure in surveillance studies and clinical trials. The purpose of this study was to describe the variability in energy expenditure for selected physical activities among adolescent girls.

Methods - Seventy-four adolescent girls (aged 13 to 14 years) participated in this cross-sectional investigation. Data were collected in 2001 and analyzed in 2004. Energy expenditure was measured by indirect calorimetry for ten activities and during a submaximal cycle ergometer test, which was used to estimate cardiorespiratory fitness. Variability in energy expended for the …


Adult Phyllophaga Ephilida Host Plant Feeding Preference, Aboubacar Diagne, Richard N. Story, Abner M. Hammond Sep 2006

Adult Phyllophaga Ephilida Host Plant Feeding Preference, Aboubacar Diagne, Richard N. Story, Abner M. Hammond

Faculty Publications

Limited biological information about Phyllophaga ephilida, a major sweet potato pest in Louisiana, is available. In 2001 and 2002, a study was conducted in the laboratory to investigate the feeding preference of adult Phyllophaga ephilida (Say) (Coleoptera: Scarabaeidae) for the foliage of eight woody plant species: water oak (Quercus nigra L.), live oak (Quercus virginiana Mill.), red maple (Acer rubrum L.), slash pine (Pinus caribaea Morelet), pecan (Carya illinoensis (Wangenh) K. Koch), sweetgum (Liquidambar styraciflua L.), southern magnolia (Magnolia grandiflora L.), and American elm (Ulmus americana L.). Beetles were placed in an arena with the eight host plants and allowed …


Promoting Physical Activity In Children And Youth: A Leadership Role For Schools: A Scientific Statement From The American Heart Association Council On Nutrition, Physical Activity, And Metabolism (Physical Activity Committee) In Collaboration With The Councils On Cardiovascular Disease In The Young And Cardiovascular Nursing, Russell R. Pate, Michael G. Davis, Thomas N. Robinson, Elaine J. Stone, Thomas L. Mckenzie, Judith C. Young Sep 2006

Promoting Physical Activity In Children And Youth: A Leadership Role For Schools: A Scientific Statement From The American Heart Association Council On Nutrition, Physical Activity, And Metabolism (Physical Activity Committee) In Collaboration With The Councils On Cardiovascular Disease In The Young And Cardiovascular Nursing, Russell R. Pate, Michael G. Davis, Thomas N. Robinson, Elaine J. Stone, Thomas L. Mckenzie, Judith C. Young

Faculty Publications

No abstract provided.


Effects Of Resistant Starch, A Non-Digestible Fermentable Fiber, On Reducing Body Fat, Michael J. Keenan, Jun Zhou, Kathleen L. Mccutcheon, Anne M. Raggio, H. Gale Bateman, Emily Todd, Christina K. Jones, Richard T. Tulley, Sheri Melton, Roy J. Martin, Maren Hegsted Sep 2006

Effects Of Resistant Starch, A Non-Digestible Fermentable Fiber, On Reducing Body Fat, Michael J. Keenan, Jun Zhou, Kathleen L. Mccutcheon, Anne M. Raggio, H. Gale Bateman, Emily Todd, Christina K. Jones, Richard T. Tulley, Sheri Melton, Roy J. Martin, Maren Hegsted

Faculty Publications

Objective: To assess the effects of energy dilution with non-fermentable and fermentable fibers on abdominal fat and gut peptide YY (PYY) and glucagon-like peptide (GLP)-1 expressions, three rat studies were conducted to: determine the effects of energy dilution with a non-ferment-able fiber, compare similar fiber levels of fermentable and non-fermentable fibers, and compare similar metabolizable energy dilutions with fermentable and non-fermentable fibers. Research Methods and Procedures: In Study 1, rats were fed one of three diets with different metabolizable energy densities. In Study 2, rats were fed diets with similar fiber levels using high amylose-resistant cornstarch (RS) or methylcellulose. In …


Intestinal Enzymes During Malnutrition & Infection In Rabbits, Sufia Islam, Amal K. Mitra, Ashish K. Chowdhury, Nur Haque Alam Sep 2006

Intestinal Enzymes During Malnutrition & Infection In Rabbits, Sufia Islam, Amal K. Mitra, Ashish K. Chowdhury, Nur Haque Alam

Faculty Publications

Background & objectives: Malnutrition plays an important role in the intestinal absorption of nutrients. However, reports are not consistent whether intestinal enzymes are decreased in the presence of malnutrition. It is also not clear whether simultaneous presence of malnutrition and infection adds to the problem of malabsorption of nutrients. The aim of the present study was to determine intestinal functions in terms of concentrations of disaccharidase enzymes during diarrhoea and protein energy malnutrition. Methods: Concentrations of three disaccharidase enzymes, namely maltase, sucrase and lactase were measured in nine energy-restricted and five control rabbits during diarrhoea induced by rabbit diarrhoeagenic Escherichia …


Interaction Of Ice Storms And Management Practices On Current Carbon Sequestration In Forests With Potential Mitigation Under Future Co2 Atmosphere, Heather R. Mccarthy, Ram Oren, Hyun-Seok Kim, Kurt H. Johnsen, Chris Maier, Seth G. Pritchard, Micheal Davis Aug 2006

Interaction Of Ice Storms And Management Practices On Current Carbon Sequestration In Forests With Potential Mitigation Under Future Co2 Atmosphere, Heather R. Mccarthy, Ram Oren, Hyun-Seok Kim, Kurt H. Johnsen, Chris Maier, Seth G. Pritchard, Micheal Davis

Faculty Publications

[1] Ice storms are disturbance events with potential impacts on carbon sequestration. Common forest management practices, such as fertilization and thinning, can change wood and stand properties and thus may change vulnerability to ice storm damage. At the same time, increasing atmospheric CO2 levels may also influence ice storm vulnerability. Here we show that a nonintensively managed pine plantation experienced a ∼250 g C m−2 reduction in living biomass during a single storm, equivalent to ∼30% of the annual net ecosystem carbon exchange of this ecosystem. Drawing on weather and damage survey data from the entire storm cell, …


Health In Cuba, Richard S. Cooper, Joan F. Kennelly, Pedro Orduñez-Garcia Aug 2006

Health In Cuba, Richard S. Cooper, Joan F. Kennelly, Pedro Orduñez-Garcia

Faculty Publications

The poorer countries of the world continue to struggle with an enormous health burden from diseases that we have long had the capacity to eliminate. Similarly, the health systems of some countries, rich and poor alike, are fragmented and inefficient, leaving many population groups underserved and often without health care access entirely. Cuba represents an important alternative example where modest infrastructure investments combined with a well-developed public health strategy have generated health status measures comparable with those of industrialized countries. Areas of success include control of infectious diseases, reduction in infant mortality, establishment of a research and biotechnology industry, and …


Quality Of Cornmeal Stored Long-Term In A Low Oxygen Atmosphere, Megan L. Bingham, Heather F. Pahulu, Lynn V. Ogden, Oscar A. Pike Jun 2006

Quality Of Cornmeal Stored Long-Term In A Low Oxygen Atmosphere, Megan L. Bingham, Heather F. Pahulu, Lynn V. Ogden, Oscar A. Pike

Faculty Publications

The U.S. Department of Homeland Security recommends food be included in efforts to be prepared for natural disasters and other emergencies. Cornmeal packaged in No. 10 cans with a low oxygen atmosphere has been available in the retail market for decades, but the effect of long-term storage on quality is unknown. This study assessed the quality of cornmeal stored up to 33 years at ambient temperatures in a low oxygen atmosphere. Sixteen samples of cornmeal packaged in No. 10 cans ranging in age from < 1 to 33 years were obtained from donors. The cornmeal and products made from the cornmeal (cornbread and cornmeal hot cereal) were evaluated by a 50-member consumer panel using a 9-point hedonic scale for attributes including appearance, aroma, flavor, texture, and overall acceptability. Acceptance for everyday use and emergency situations was also determined. Additional measurements included can headspace oxygen, can seam integrity, color, and water activity. There was no significant decrease over storage time in aroma, texture, flavor, and overall acceptability in cornmeal, cornbread or cornmeal cereal. Hedonic scores for cornmeal aroma and appearance ranged from 5.2 to 6.3 and 5.6 to 7.0, respectively. Hedonic scores for overall acceptability of cornmeal cereal and corn bread varied from 4.5 to 6.0 and 6.0 to 7.1, respectively. Acceptance of the cornmeal in a regular diet ranged from 44 to 76 % and emergency acceptance ranged from 90 to 99 %. Headspace oxygen ranged from 0.309 to 20.4%. CIE L*a*b* color values ranged from 82.0-86.6, 3.2-10.1, and 26.3-50.8, respectively. Cornmeal appearance hedonic scores correlated with CIE b* color values (r2=0.75). Water activity ranged from .28 to .58. Cornmeal, especially as an ingredient in a recipe, is capable of retaining a high percentage of consumer acceptance over long periods of time and can be included in long-term food storage efforts.


Association Of Physical Activity With Hormone Receptor Status: The Shanghai Breast Cancer Study, Swann Arp Adams, Charles E. Matthews, James R. Hébert, Charity G. Moore, Joan E. Cunningham, Xiou-Oi Shu, Jeanette Fulton, Yutang Gao, Wei Zheng Jun 2006

Association Of Physical Activity With Hormone Receptor Status: The Shanghai Breast Cancer Study, Swann Arp Adams, Charles E. Matthews, James R. Hébert, Charity G. Moore, Joan E. Cunningham, Xiou-Oi Shu, Jeanette Fulton, Yutang Gao, Wei Zheng

Faculty Publications

Evidence exists that breast tumors differing by estrogen receptor (ER) and progesterone receptor (PR) status may be phenotypically distinct diseases resulting from dissimilar etiologic processes. Few studies have attempted to examine the association of physical activity with breast cancer subtype. Such research may prove instructive into the biological mechanisms of activity. Consequently, this investigation was designed to assess the relationship between physical activity and hormone receptor-defined breast cancers in a population of Asian women in which the distribution of receptor types differed from traditional Western populations. Participants, ages 25 to 64 years, were recruited into this population-based, case-control study of …


Aha/Acc Guidelines For Secondary Prevention For Patients With Coronary And Other Atherosclerotic Vascular Disease: 2006 Update, Sidney C. Smith Jr., Jerilyn Allen, Steven N. Blair, Robert O. Bonow, Lawrence M. Brass, Gregg C. Fonarow, Scott M. Grundy, Loren Hiratzka, Daniel Jones, Harlan M. Krumholz, Lori Mosca, Richard C. Pasternak, Thomas A. Pearson, Marc A. Pfeffer, Kathryn A. Taubert May 2006

Aha/Acc Guidelines For Secondary Prevention For Patients With Coronary And Other Atherosclerotic Vascular Disease: 2006 Update, Sidney C. Smith Jr., Jerilyn Allen, Steven N. Blair, Robert O. Bonow, Lawrence M. Brass, Gregg C. Fonarow, Scott M. Grundy, Loren Hiratzka, Daniel Jones, Harlan M. Krumholz, Lori Mosca, Richard C. Pasternak, Thomas A. Pearson, Marc A. Pfeffer, Kathryn A. Taubert

Faculty Publications

No abstract provided.


Positive Association Between Resting Energy Expenditure And Weight Gain In A Lean Adult Population, Amy Luke, Ramon Durazo-Arvizu, Guichan Cao, Adebowale Adeyemo, Bamidele Tayo, Richard Cooper May 2006

Positive Association Between Resting Energy Expenditure And Weight Gain In A Lean Adult Population, Amy Luke, Ramon Durazo-Arvizu, Guichan Cao, Adebowale Adeyemo, Bamidele Tayo, Richard Cooper

Faculty Publications

Background: Weight gain in adulthood is common, from modest gains in developing countries to substantial increases in Western societies. Evidence of the importance of energy expenditure in adult weight change has been limited to studies conducted in Pima Indians, in whom resting energy expenditure (REE) was found to be inversely associated with weight gain. Objective: The aim was to determine whether REE was predictive of weight change in lean Nigerian adults. Design: Weight was measured in 744 adults on 2-4 occasions over 5.5 y. REE was measured in the second follow-up examination. Sex-specific, mixed-effects models with REE, fat-free mass, and …


Mid-Season Prediction Of Wheat-Grain Yield Potential Using Plant, Soil, And Sensor Measurements, K. Girma, K. L. Martin, R. H. Anderson, D. B. Arnall, K. D. Brixey, M. A. Casillas, B. Chung, B. C. Dobey, S. K. Kamenidou, S. K. Kariuki, E. E. Katsalirou, J. C. Morris, J. Q. Moss, C. T. Rohla, B. J. Sudbury, B. S. Tubana, W. R. Raun May 2006

Mid-Season Prediction Of Wheat-Grain Yield Potential Using Plant, Soil, And Sensor Measurements, K. Girma, K. L. Martin, R. H. Anderson, D. B. Arnall, K. D. Brixey, M. A. Casillas, B. Chung, B. C. Dobey, S. K. Kamenidou, S. K. Kariuki, E. E. Katsalirou, J. C. Morris, J. Q. Moss, C. T. Rohla, B. J. Sudbury, B. S. Tubana, W. R. Raun

Faculty Publications

The components that define cereal-grain yield potential have not been well defined. The objective of this study was to collect many differing biological measurements from a long-term winter wheat (Triticum aestivum L.) study in an attempt to better define yield potential. Four treatments were sampled that annually received 0, 45, 90, and 135 kg N ha-1 at fixed rates of phosphorus (P) (30 kg ha-1) and potassium (K) (37 kg ha -1). Mid-season measurements of leaf color, chlorophyll, normalized difference vegetative index (NDVI), plant height, canopy temperature, tiller density, plant density, soil moisture, soil NH4-N, NO3-N, organic carbon (C), total …


Resisting Deep Capture: The Commercial Speech Doctrine And Junk-Food Advertising To Children, David Yosifon May 2006

Resisting Deep Capture: The Commercial Speech Doctrine And Junk-Food Advertising To Children, David Yosifon

Faculty Publications

The present Article is more precisely dedicated to analyzing, from a critical realist perspective, the wisdom and constitutional viability of one possible policy response to the obesity crisis: a ban on junk-food advertising to children.

This Article seeks not only to show that an effective junk-food advertising ban could pass constitutional scrutiny, but also to demonstrate, through the rigor of a constitutional analysis, the wisdom of such an approach to this substantial social problem. Simultaneously, my purpose is to show, in the context of a difficult First Amendment question, that the critical realist approach to legal theory is capable of …


Antagonisms, Mutualisms And Commensalisms Affect Outbreak Dynamics Of The Southern Pine Beetle, Richard W. Hofstetter, James T. Cronin, Kier D. Klepzig, John C. Moser, Matthew P. Ayres Apr 2006

Antagonisms, Mutualisms And Commensalisms Affect Outbreak Dynamics Of The Southern Pine Beetle, Richard W. Hofstetter, James T. Cronin, Kier D. Klepzig, John C. Moser, Matthew P. Ayres

Faculty Publications

Feedback from community interactions involving mutualisms are a rarely explored mechanism for generating complex population dynamics. We examined the effects of two linked mutualisms on the population dynamics of a beetle that exhibits outbreak dynamics. One mutualism involves an obligate association between the bark beetle, Dendroctonus frontalis and two mycangial fungi. The second mutualism involves Tarsonemus mites that are phoretic on D. frontalis ("commensal"), and a blue-staining fungus, Ophiostoma minus. The presence of O. minus reduces beetle larval survival ("antagonistic") by outcompeting beetle-mutualistic fungi within trees yet supports mite populations by acting as a nutritional mutualist. These linked interactions potentially …


Peptide Yy And Proglucagon Mrna Expression Patterns And Regulation In The Gut, Jun Zhou, Maren Hegsted, Kathleen L. Mccutcheon, Michael J. Keenan, Aochun Xi, Anne M. Raggio, Roy J. Martin Apr 2006

Peptide Yy And Proglucagon Mrna Expression Patterns And Regulation In The Gut, Jun Zhou, Maren Hegsted, Kathleen L. Mccutcheon, Michael J. Keenan, Aochun Xi, Anne M. Raggio, Roy J. Martin

Faculty Publications

Objective: Peptide YY (PYY) and glucagon-like peptide-1 are important in the control of energy homeostasis and are both secreted from the gut in response to ingested nutrients. However, more studies are needed on nutrient regulation of their gene expression patterns in specific areas of the gut. This study detailed PYY and proglucagon (the gene that encodes glucagon-like peptide-1) gene expression patterns and regulation in the gut. We further examined the regulation of PYY and proglucagon mRNA by a diet containing fermentation- resistant starch (in vivo) and butyrate (in vitro). Research Methods and Procedures: Quantitative real time reverse transcriptase-polymerase chain reaction …


Seasonal Variation In Food Intake, Physical Activity, And Body Weight In A Predominantly Overweight Population, Yunsheng Ma, Barbara C. Olendzki, W. Li, Andrea R. Hafner, David E. Chiriboga, James R. Hébert, Mary Jane Campbell, M. Sarnie, Ira S. Ockene Apr 2006

Seasonal Variation In Food Intake, Physical Activity, And Body Weight In A Predominantly Overweight Population, Yunsheng Ma, Barbara C. Olendzki, W. Li, Andrea R. Hafner, David E. Chiriboga, James R. Hébert, Mary Jane Campbell, M. Sarnie, Ira S. Ockene

Faculty Publications

OBJECTIVE:

To describe seasonal variation in food intake, physical activity, and body weight in a predominantly overweight population.

DESIGN:

A longitudinal observational study.

SETTING:

Most of the study participants were recruited from a health maintenance organization (HMO) in central Massachusetts, USA. Additional individuals of Hispanic descent were recruited from outside of the HMO population to increase the ethnic diversity of this sample.

SUBJECTS:

Data from 593 participants, aged 20-70, were used for this investigation. Each participant was followed quarterly (five sampling points: baseline and four consecutive quarters) for 1-year period. Body weight measurements and three 24-h dietary and physical activity …


Association Between Tobacco Use And Body Mass Index In Urban Indian Population: Implications For Public Health In India, Mangesh S. Pednekar, Prakash C. Gupta, Heema C. Shukla, James R. Hébert Mar 2006

Association Between Tobacco Use And Body Mass Index In Urban Indian Population: Implications For Public Health In India, Mangesh S. Pednekar, Prakash C. Gupta, Heema C. Shukla, James R. Hébert

Faculty Publications

Background: Body mass index [BMI, weight (kg)/height (m2)], a measure of relative weight, is a good overall indicator of nutritional status and predictor of overall health. As in many developing countries, the high prevalence of very low BMIs in India represents an important public health risk. Tobacco, smoked in the form of cigarettes or bidis (handmade by rolling a dried rectangular piece of temburni leaf with 0.15–0.25 g of tobacco) or chewed, is another important determinant of health. Tobacco use also may exert a strong influence on BMI.

Methods: The relationship between very low BMI (< 18.5 kg/m2) and tobacco use was examined using data from a representative cross-sectional survey of 99,598 adults (40,071 men and 59,527 women) carried out in the city of Mumbai (formerly known as Bombay) in western India. Participants were men and women aged ≥ 35 years who were residents of the main city of Mumbai.

Results: …


Racially-Tailored’ Medicine Unraveled, Sharona Hoffman Mar 2006

Racially-Tailored’ Medicine Unraveled, Sharona Hoffman

Faculty Publications

In June 2005, the FDA approved BiDil, a heart failure medication that is labeled for use only by African-Americans and thus is the first treatment of its kind. The drug likely portends a future of growing interest in "race-based" medicine. This phenomenon is emerging at the same time that scientists, in light of the Human Genome Project, are reaching an understanding that "race" has no biological meaning, and consequently, "racially-tailored" medicine is both puzzling and troubling.

This Article explores the reasons for the new focus on "racial-profiling" in medicine. It analyzes the risks and dangers of this approach, including medical …


Anthelmintic Resistance Of Gastrointestinal Parasites In Small Ruminants, Sherrill A. Fleming, Tom Craig, Ray M. Kaplan, James E. Miller, Christine Navarre, Mike Rings Mar 2006

Anthelmintic Resistance Of Gastrointestinal Parasites In Small Ruminants, Sherrill A. Fleming, Tom Craig, Ray M. Kaplan, James E. Miller, Christine Navarre, Mike Rings

Faculty Publications

No abstract provided.


Anthelmintic Resistance Of Gastrointestinal Parasites In Small Ruminants, Sherrill A. Fleming, Tom Craig, Ray M. Kaplan, James E. Miller, Christine Navarre, Mike Rings Mar 2006

Anthelmintic Resistance Of Gastrointestinal Parasites In Small Ruminants, Sherrill A. Fleming, Tom Craig, Ray M. Kaplan, James E. Miller, Christine Navarre, Mike Rings

Faculty Publications

No abstract provided.


Anthelmintic Resistance Of Gastrointestinal Parasites In Small Ruminants, Sherrill A. Fleming, Tom Craig, Ray M. Kaplan, James E. Miller, Christine Navarre, Mike Rings Mar 2006

Anthelmintic Resistance Of Gastrointestinal Parasites In Small Ruminants, Sherrill A. Fleming, Tom Craig, Ray M. Kaplan, James E. Miller, Christine Navarre, Mike Rings

Faculty Publications

No abstract provided.


Epithelial Sodium Channel Allele T594m Is Not Associated With Blood Pressure Or Blood Pressure Response To Amiloride, John M. Hollier, Dan F. Martin, Darin M. Bell, Jia Ling Li, Mike G. Chirachanchai, Dileep V. Menon, David Leonard, Xiaodong Wu, Richard S. Cooper, Colin Mckenzie, Ronald G. Victor, Richard J. Auchus Mar 2006

Epithelial Sodium Channel Allele T594m Is Not Associated With Blood Pressure Or Blood Pressure Response To Amiloride, John M. Hollier, Dan F. Martin, Darin M. Bell, Jia Ling Li, Mike G. Chirachanchai, Dileep V. Menon, David Leonard, Xiaodong Wu, Richard S. Cooper, Colin Mckenzie, Ronald G. Victor, Richard J. Auchus

Faculty Publications

The T594M allele of the epithelial sodium channel β-subunit has been proposed as a gain-of-function mutation leading to salt-sensitive hypertension in blacks that is particularly responsive to the specific sodium channel antagonist amiloride. However, the positive associations derive from small convenience samples, and the amiloride challenge study lacked a control group. We determined whether the T594M allele was associated with hypertension and blood pressure (BP) response to amiloride in 2 well-characterized random population samples including 3137 Dallas County subjects and 1666 Jamaican blacks. In multivariate models, the T594M allele was not predictive of systolic BP (adjusted odds ratio for hypertension …


Interfacing Capillary Gel Microfluidic Chips With Infrared Laser Desorption Mass Spectrometry, Yichuan Xu, Mark W. Little, Kermit K. Murray Mar 2006

Interfacing Capillary Gel Microfluidic Chips With Infrared Laser Desorption Mass Spectrometry, Yichuan Xu, Mark W. Little, Kermit K. Murray

Faculty Publications

We report on the fabrication and performance of a gel microfluidic chip interfaced to laser desorption/ionization (LDI) mass spectrometry with a time-of-flight mass analyzer. The chip was fabricated from poly(methylmethacrylate) with a poly(dimethyl siloxane) cover. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed in the channel of the microfluidic chip. After electrophoresis, the cover was removed and either the PDMS chip or the PMMA cover was mounted in a modified MALDI ion source for analysis. Ions were formed by irradiating the channel with 2.95 μm radiation from a pulsed optical parametric oscillator (OPO), which is coincident with IR absorption by N-H …


Coprophagy In A Cave-Adapted Salamander; The Importance Of Bat Guano Examined Through Nutritional And Stable Isotope Analyses, Danté B. Fenolio, G. O. Graening, Bret A. Collier, Jim F. Stout Feb 2006

Coprophagy In A Cave-Adapted Salamander; The Importance Of Bat Guano Examined Through Nutritional And Stable Isotope Analyses, Danté B. Fenolio, G. O. Graening, Bret A. Collier, Jim F. Stout

Faculty Publications

During a two year population ecology study in a cave environment, 15 Eurycea (=Typhlotriton) spelaea were observed ingesting bat guano. Furthermore, E. spelaea capture numbers increased significantly during the time that grey bats (Myotis grisescens) deposited fresh guano. We investigated the hypothesis that this behaviour was not incidental to the capture of invertebrate prey, but a diet switch to an energy-rich detritus in an oligotrophic environment. Stable isotope assays determined that guano may be assimilated into salamander muscle tissue, and nutritional analyses revealed that guano is a comparable food source to potential invertebrate prey items. This is the first report …


The Prp19 U-Box Crystal Structure Suggests A Common Dimeric Architecture For A Class Of Oligomeric E3 Ubiquitin Ligases, Craig W. Vander Kooi, Melanie D. Ohi, Joshua A. Rosenberg, Michael L. Oldham, Marcia E. Newcomer, Kathleen L. Gould, Walter J. Chazin Jan 2006

The Prp19 U-Box Crystal Structure Suggests A Common Dimeric Architecture For A Class Of Oligomeric E3 Ubiquitin Ligases, Craig W. Vander Kooi, Melanie D. Ohi, Joshua A. Rosenberg, Michael L. Oldham, Marcia E. Newcomer, Kathleen L. Gould, Walter J. Chazin

Faculty Publications

Prp19 is an essential splicing factor and a member of the U-box family of E3 ubiquitin ligases. Prp19 forms a tetramer via a central coiled-coil domain. Here, we show the U-box domain of Prp19 exists as a dimer within the context of the Prp19 tetramer. A high-resolution structure of the homodimeric state of the Prp19 U-box was determined by X-ray crystallography. Mutation of the U-box dimer interface abrogates U-box dimer formation and is lethal in vivo. The structure of the U-box dimer enables construction of a complete model of Prp19 providing insights into how the tetrameric protein functions as an …