Digital Commons Network^™

Sex Differences In Rhesus Monkeys’ Digit Ratio (2d:4d Ratio) And Its Association With Maternal Social Dominance Rank, Alexander Baxter, Elizabeth K. Wood, Parker Jarman, Ashley N. Cameron, John P. Capitanio, James Dee Higley

Faculty Publications

Prenatal androgen exposure (PAE) plays a pivotal role in masculinizing the developing body and brain, and extreme exposure may contribute to autism, anxiety disorder and schizophrenia. One commonly used biomarker for PAE is the pointer-to-ring-finger digit length (2D:4D) ratio. Although this biomarker is widely used in human studies, relatively few studies have investigated 2D:4D ratio in nonhuman primates, particularly rhesus macaques (Macaca mulatta), one of the most commonly used animals in biomedical research. Thus far, data suggest that sexual dimorphism in 2D:4D ratio may be in the opposite direction in some monkey species, when compared to the pattern …

Advancing Social Connection As A Public Health Priority In The United States, Julianne Holt-Lunstad, Theodore Robles, David A. Sbarra

Faculty Publications

A robust body of scientific evidence indicates that being embedded in high-quality close relationships and feeling socially connected to the people in your life is associated with decreased risk for all-cause mortality as well as a range of disease morbidities. Despite mounting evidence that the magnitude of these associations is comparable to many leading health determinants (that receive significant public health resources), government agencies, healthcare providers and associations, and public/private healthcare funders are slow to recognize human social relationships as either a health determinant or health risk marker in a manner that is comparable to other public health priorities. This …

Ending Domestic Violence--No Time To Waste, Beverly J. Sedlacek, David Sedlacek

Faculty Publications

The church plays an important role in creating a safe community and an environment in which intimate partner violence (IPV) is addressed, not covered up. An appropriate understanding of Scripture is also protective. Local churches must become aware of resources in their communities designed to help both victims and perpetrators. The message that violence of any kind will not be tolerated must be clearly sent. Perpetrators must experience appropriate consequences along with the help they need. enditnow, the Adventist Church’s program to create awareness and help prevent IPV, must continue to be supported.

Near-Infrared Photothermal Release Of Sirna From The Surface Of Colloidal Gold-Silver-Gold Core-Shell-Shell Nanoparticles Studied With Second-Harmonic Generation, Raju R. Kumal, Mohammad Abu-Laban, Prakash Hamal, Blake Kruger, Holden T. Smith, Daniel J. Hayes, Louis H. Haber

Faculty Publications

© 2018 American Chemical Society. Photothermal release of oligonucleotides from the surface of plasmonic nanoparticles represents a promising platform for spatiotemporal controlled drug delivery. Here we demonstrate the use of novel gold-silver-gold core-shell-shell (CSS) nanoparticles to study the photothermal cleaving and release of micro-RNA (miRNA) mimics or small interfering RNA (siRNA) under near-infrared (NIR) irradiation. The furan-maleimide-based Diels-Alder adduct cleaves thermally above 60 °C and is used to bind siRNA to the colloidal nanoparticle surface in water. We investigate the photothermal cleaving kinetics of siRNA under different NIR laser powers using surface-sensitive time-dependent second-harmonic generation (SHG) spectroscopy. The photothermal release …

Evaluation Of Self-Report Adherence Measures And Their Associations With Detectable Viral Load Among People Living With Hiv (Plhiv) In China, Wendi Da, Xiaoming Li Ph.D., Shan Qiao Ph.D., Yuejiao Zhou, Zhiyong Shen

Faculty Publications

Objectives Self-report antiretroviral therapy (ART) adherence has been consistently associated with clinical outcomes. This study aims to compare the accuracy of self-report ART adherence measures with varying recall timeframes or item contents to predict virological response.

Methods Data from a cross-sectional study among 2146 participants on ART in Guangxi, China were used. Detectable viral load was defined as viral load > 50 copies/ml. Adherence was measured using the number of days on which all doses were taken in the past month (i.e., the “one-month days taken” measure), the number of days on which any dose was missed in the past month …

Integrating Climate Forecasts With The Soil And Water Assessment Tool (Swat) For High-Resolution Hydrologic Simulations And Forecasts In The Southeastern U.S., Vinit Sehgal, Venkataramana Sridhar, Luke Juran, Jactone Arogo Ogejo

Faculty Publications

This study provides high-resolution modeling of daily water budget components at Hydrologic Unit Code (HUC)-12 resolution for 50 watersheds of the South Atlantic Gulf (SAG) region in the southeastern U.S. (SEUS) by implementing the Soil and Water Assessment Tool (SWAT) model in the form of a near real-time, semi-automated framework. A near real-time hydrologic simulation framework is implemented with a lead time of nine months (March-December 2017) by integrating the calibrated SWAT model with National Centers for Environmental Prediction coupled forecast system model version 2 (CFSv2) weather data to forecast daily water balance components. The modeling exercise is conducted as …

Large-Scale Social And Behavior Change Communication Interventions Have Sustained Impacts On Infant And Young Child Feeding Knowledge And Practices: Results Of A 2-Year Follow-Up Study In Bangladesh, Sunny S. Kim, Phuong Hong Nguyen, Lan Mai Tran, Tina Sanghvi, Zeba Mahmud, Mohammad Raisul Haque, Kaosar Afsana, Edward A. Frongillo Jr., Marie T. Ruel, Purnima Menon

Faculty Publications

Background: Sustained improvements in infant and young child feeding (IYCF) require continued implementation of effective interventions. From 2010-2014, Alive & Thrive (A&T) provided intensive interpersonal counseling (IPC), community mobilization (CM), and mass media (MM) in Bangladesh, demonstrating impact on IYCF practices. Since 2014, implementation has been continued and scaled up by national partners with support from other donors and with modifications such as added focus on maternal nutrition and reduced program intensity. Objective: We assessed changes in intervention exposure and IYCF knowledge and practices in the intensive (IPC + CM + MM) compared with nonintensive areas (standard nutrition counseling + …

Experimental Investigation Of Impact Localization In Composite Plate Using Newly Developed Imaging Method, Mohammad Faisal Haider, Asaad Migot, Md Yeasin Bhuiyan, Victor Giurgiutiu

Faculty Publications

This paper focuses on impact localization of composite structures, which possess more complexity in the guided wave propagation due to the anisotropic behavior of composite materials. In this work, a composite plate was manufactured by using a compression molding process with proper pressure and temperature cycle. Eight layers of woven composite prepreg were used to manufacture the composite plate. A structural health monitoring (SHM) technique was implemented with piezoelectric wafer active sensors (PWAS) to detect and localize the impact on the plate. There were two types of impact event that were considered in this paper (a) low energy impact event …

Nicu Nurses' Suggestions For Improving End-Of-Life Care Obstacles, Renea L. Beckstrand, Rebecca F. Isaacson, Janelle L. B. Macintosh, Karlen E. Luthy, Lacey Eden

Faculty Publications

Background: Neonatal deaths (infants less than 28 days old) account for two thirds (66.7%) of all infant deaths with most occurring in an ICU setting. NICU nurses are frequently involved in end-of-life (EOL) care and face unique obstacles.

Objective: The objective of this study was to obtain NICU nurses’ suggestions for improving obstacles in EOL care in NICUs.

Methods: Suggestions were obtained through mailed survey research in qualitative study design. Returned surveys yielded 121 nurse respondents who gave a total of 138 suggestions.

Results: A total of 10 cohesive themes were identified: (1) environmental design issues, (2) improved communication between …

Awareness And Knowledge Of Cochlear Implants Among Speech-Language Pathologists, Kimberly Ward, Kimberly Grubbs, Amitava Biswas

Faculty Publications

Speech-Language Pathologists work closely with individuals who have received cochlear implants across a multitude of settings which include early childhood education centers, schools, hospitals and skilled nursing facilities. However, previous research suggests that Speech-Language Pathologists do not have the adequate skills or knowledge to work with clients who have received cochlear implants. To assess whether Speech-Language Pathologists in the state of Mississippi had similar results as in the previous studies, a questionnaire was used to assess knowledge on cochlear implants. The results are consistent with previous studies which suggest that there is an overall lack in cochlear implant competency, specifically …

Curvilinear Association Between Language Disfluency And Fmr1 Cgg Repeat Size Across The Normal, Intermediate, And Premutation Range, Jessica Klusek, Ann Porter, Leonard Abbeduto, Tatyana Adayev, Flora Tassone, Marsha R. Mailick, Anne Glicksman, Bridgette L. Tonnsen, Jane E. Roberts

Faculty Publications

Historically, investigations of FMR1 have focused almost exclusively on the clinical effects of CGG expansion within the categories of the premutation (55–200 CGG repeats) and fragile X syndrome (>200 CGG repeats). However, emerging evidence suggests that CGG-dependent phenotypes may occur across allele sizes traditionally considered within the “normal” range. This study adopted an individual-differences approach to determine the association between language production ability and CGG repeat length across the full range of normal, intermediate, and premutation alleles. Participants included 61 adult women with CGG repeats within the premutation (n = 37), intermediate (i.e., 41–54 repeats; n = 2), …

Lessons Learned After A Disaster: Investigations Of Public Librarians’ Health Information Services To The Community And Community Members’ Information Needs Following A Catastrophic Flood, Feili Tu-Keefner, Jingjing Liu, Darin Freeburg, Elizabeth Hartnett, Denise Lyons, Mike Corbo, April Hobbs

Faculty Publications

In times of crisis, public libraries, in addition to health, fire, and police departments, are community outreach centers and sources of credible information. In October 2015, several counties in the state of South Carolina in the United States of America experienced catastrophic flooding that caused severe damage. Situation-specific research was conducted to investigate public libraries’ value to their communities in providing critical information services, as well as the libraries’ legitimacy as partners of public health agencies. The research framework is one recommended by public health experts for risk communication preparedness and implementation during pandemic influenza. In 2015, a case study …

Modeling The Influence Of Eutrophication And Redox Conditions On Mercury Cycling At The Sediment-Water Interface In The Berre Lagoon, Svetlana Pakhomova, Evgeniy Yakushev, Elizaveta Protsenko, Sylvain Rigaud, Daniel Cossa, Joel Knoery, Raoul Marie Couture, Olivier Radakovitch, Shamil Yakubov, Dominika Krzeminska, Alice Newton

Faculty Publications

© 2018 Pakhomova, Yakushev, Protsenko, Rigaud, Cossa, Knoery, Couture, Radakovitch, Yakubov, Krzeminska and Newton. This study presents a specifically designed Mercury module in a coupled benthic-pelagic reactive-transport model - Bottom RedOx Model (BROM) that allows to study mercury (Hg) biogeochemistry under different conditions. This module considers the transformation of elemental mercury (Hg(0)), divalent mercury (Hg(II)) and methylmercury (MeHg). The behavior of mercury species in the model is interconnected with changes of oxygen, hydrogen sulfide, iron oxides, organic matter, and biota. We simulated the transformation and transport of Hg species in the water column and upper sediment layer under five different …

Academic Response To Storm-Related Natural Disasters—Lessons Learned, Jerris R. Hedges, Karam F.A. Soliman, Gene D’Amour, Dong Liang, Carlos E. Rodríguez-Díaz, Kenira Thompson, Josefina Romaguera, Silvia E.Rabionet Sabater, Richard Yanagihara

Faculty Publications

On 30 October 2017, selected faculty and administrators from Research Centers in Minority Institutions (RCMI) grantee institutions gathered to share first-hand accounts of the devastating impact of Hurricanes Harvey, Irma, and Maria, which had interrupted academic activities, including research, education, and training in Puerto Rico, Florida, and Texas. The presenters reviewed emergency response measures taken by their institutions to maintain community health care access and delivery, the storm-related impact on clinical and research infrastructure, and strategies to retain locally grown clinical expertise and translational science research talent in the aftermath of natural disasters. A longer-term perspective was provided through a …

Roles Of Ros, Nrf2, And Autophagy In Cadmium-Carcinogenesis And Its Prevention By Sulforaphane, Yuting Wang, Ardhendu Kumar Mandal, Young O.K. Son, Poyil Pratheeshkumar, James T.F. Wise, Lei Wang, Zhuo Zhang, Xianglin Shi, Zhimin Chen

Faculty Publications

Environmental and occupational exposures to cadmium increase the risk of various cancers, including lung cancer. The carcinogenic mechanism of cadmium, including its prevention remains to be investigated. Using fluorescence and electron spin resonance spin trapping, the present study shows that in immortalized lung cells (BEAS-2BR cells), exposure cadmium generated reactive oxygen species (ROS). Through ROS generation, cadmium increased the protein level of TNF-α which activated NF-κB and its target protein COX-2, creating an inflammatory microenvironment. As measured by anchorage-independent colony formation assay, cadmium induced malignant cell transformation. Inhibition of ROS by antioxidants inhibited transformation, showing that ROS were important in …

A Distributed Semi-Supervised Platform For Dnase-Seq Data Analytics Using Deep Generative Convolutional Networks, Shayan Shams, Richard Platania, Joohyun Kim, Jian Zhang, Kisung Lee, Seungwon Yang, Seung Jong Park

Faculty Publications

A deep learning approach for analyzing DNase-seq datasets is presented, which has promising potentials for unraveling biological underpinnings on transcription regulation mechanisms. Further understanding of these mechanisms can lead to important advances in life sciences in general and drug, biomarker discovery, and cancer research in particular. Motivated by recent remarkable advances in the field of deep learning, we developed a platform, Deep Semi-Supervised DNase-seq Analytics (DSSDA). Primarily empowered by deep generative Convolutional Networks (ConvNets), the most notable aspect is the capability of semi-supervised learning, which is highly beneficial for common biological settings often plagued with a less sufficient number of …

A Distributed Semi-Supervised Platform For Dnase-Seq Data Analytics Using Deep Generative Convolutional Networks, Shayan Shams, Richard Platania, Joohyun Kim, Jian Zhang, Kisung Lee, Seungwon Yang, Seung Jong Park

Faculty Publications

A deep learning approach for analyzing DNase-seq datasets is presented, which has promising potentials for unraveling biological underpinnings on transcription regulation mechanisms. Further understanding of these mechanisms can lead to important advances in life sciences in general and drug, biomarker discovery, and cancer research in particular. Motivated by recent remarkable advances in the field of deep learning, we developed a platform, Deep Semi-Supervised DNase-seq Analytics (DSSDA). Primarily empowered by deep generative Convolutional Networks (ConvNets), the most notable aspect is the capability of semi-supervised learning, which is highly beneficial for common biological settings often plagued with a less sufficient number of …

The Tick Endosymbiont Candidatus Midichloria Mitochondrii And Selenoproteins Are Essential For The Growth Of Rickettsia Parkeri In The Gulf Coast Tick Vector, Khemraj Budachetri, Deepak Kumar, Gary Crispell, Christine Beck, Gregory Dasch, Shahid Karim

Faculty Publications

Background

Pathogen colonization inside tick tissues is a significant aspect of the overall competence of a vector. Amblyomma maculatum is a competent vector of the spotted fever group rickettsiae, Rickettsia parkeri. When R. parkeri colonizes its tick host, it has the opportunity to dynamically interact with not just its host but with the endosymbionts living within it, and this enables it to modulate the tick’s defenses by regulating tick gene expression. The microbiome in A. maculatum is dominated by two endosymbiont microbes: a Francisella-like endosymbiont (FLE) and CandidatusMidichloria mitochondrii (CMM). A range of selenium-containing proteins (selenoproteins) in A. …

Ambivalence: A Key To Clinical Trial Participation?, Janice A. Chilton, Monica L. Rasmus, Jay Lytton, Charles D. Kaplan, Lovell A. Jones, Thelma C. Hurd

Faculty Publications

Trust exerts a multidimensional influence at the interpersonal level in the clinical trials setting. Trust and distrust are dynamic states that are impacted, either positively or negatively, with each participant-clinical trials team interaction. Currently, accepted models of trust posit that trust and distrust coexist and their effects on engagement and retention in clinical trials are mediated by ambivalence. While understanding of trust has been informed by a robust body of work, the role of distrust and ambivalence in the trust building process are less well understood. Furthermore, the role of ambivalence and its relationship to trust and distrust in the …

Attitudes Toward Evidence-Based Practice, Occupational Stress And Work-Related Social Support Among Health Care Providers In China: A Sem Analysis, Shan Qiao Ph.D., Xiaoming Li Ph.D., Yuejiao Zhou, Zhiyong Shen, Bonita Stanton

Faculty Publications

Individuals’ attitudes toward evidence-based practices (EBP) are critical in adopting, implementing and maintaining the EBP in clinical settings. Multiple empirical studies have examined how work context may shape perceptions and attitudes towards EBP. The current study aims to further explore how both work and family contexts, as assessed by three psychosocial indicators (i.e., occupational stress, work-related social support from coworkers, and work-related social support from family), may affect attitudes toward EBP among health care providers in HIV clinics in China. We analyzed cross-sectional survey data from 357 health care providers recruited from 40 HIV clinics across 16 cities/counties in Guangxi …

Congenital Zika Virus Infection In Immunocompetent Mice Causes Postnatal Growth Impediment And Neurobehavioral Deficits, Fengwei Bai, Amber Paul, Dhiraj Acharya, Biswas Neupane, E.A. Thompson, Gabriel Gonzalez-Fernandez, Katherine Copeland, Melanae Garrett, Haibei Liu, Mariper Lopez, Matthew De Cruz, Alex S. Flynt, Jun Liao, Yan-Lin Guo, Federico Gonzalez-Fernandez, Parminder Vig

Faculty Publications

A small percentage of babies born to Zika virus (ZIKV)-infected mothers manifest severe defects at birth, including microcephaly. Among those who appeared healthy at birth, there are increasing reports of postnatal growth or developmental defects. However, the impact of congenital ZIKV infection in postnatal development is poorly understood. Here, we report that a mild congenital ZIKV-infection in pups born to immunocompetent pregnant mice did not display apparent defects at birth, but manifested postnatal growth impediments and neurobehavioral deficits, which include reduced locomotor and cognitive deficits that persisted into adulthood. We found that the brains of these pups were smaller, had …

Structure Based Modulation Of Electron Dynamics In Meso-(4-Pyridyl)-Bodipys: A Computational And Synthetic Approach, Daniel J. Lamaster, Nichole E.M. Kaufman, Adam S. Bruner, M. Graça H. Vicente

Faculty Publications

© 2018 American Chemical Society. The effects of structural modification on the electronic structure and electron dynamics of cationic meso-(4-pyridyl)-BODIPYs were investigated. A library of 2,6-difunctionalized meso-(4-pyridyl)-BODIPYs bearing various electron-withdrawing substituents was designed, and DFT calculations were used to model the redox properties, while TDDFT was used to determine the effects of functionalization on the excited states. Structural modification was able to restructure the low-lying molecular orbitals to effectively inhibit d-PeT. A new meso-(4-pyridyl)-BODIPY bearing 2,6-dichloro groups was synthesized and shown to exhibit enhanced charge recombination fluorescence. The fluorescence enhancement was determined to be the result of functionalization modulating the …

Why "Trauma-Related Dissociation" Is A Misnomer In Courts: A Critical Analysis Of Brand Et Al. (2017a, B), Harald Merckelbach, Lawrence Patihis

Faculty Publications

Forensic psychologists are sometimes faced with the task of educating triers of fact about the evidential weight of dissociative experiences reported by claimants in litigation procedures. In their two-part essay, Brand et al. (Psychological Injury and Law, 10, 283–297, 2017a; Psychological Injury and Law, 10, 298–312, 2017b) provide advice to experts who find themselves in such situation. We argue that the Brand et al. approach is problematic and might induce confirmation bias in experts. Their approach is not well connected to the extant literature on recovered memories, dissociative amnesia, memory distortions, and symptom validity testing. In some …

Dietary Inflammatory Index And Biomarkers Of Lipoprotein Metabolism, Inflammation And Glucose Homeostasis In Adults, Catherine Phillips, Nitin Shivappa, James R. Hébert, Ivan Perry

Faculty Publications

Accumulating evidence identifies diet and inflammation as potential mechanisms contributing to cardiometabolic risk. However, inconsistent reports regarding dietary inflammatory potential, biomarkers of cardiometabolic health and metabolic syndrome (MetS) risk exist. Our objective was to examine the relationships between a food frequency questionnaire (FFQ)-derived dietary inflammatory index (DII®), biomarkers of lipoprotein metabolism, inflammation and glucose homeostasis and MetS risk in a cross-sectional sample of 1992 adults. Energy-adjusted DII (E-DII) scores derived from an FFQ were calculated. Lipoprotein particle size and subclass concentrations were measured using nuclear magnetic resonance (NMR) spectroscopy. Serum acute-phase reactants, adipocytokines, pro-inflammatory cytokines and white blood cell (WBC) …

The Effect Of E-Book Vocabulary Instruction On Spanish-English Speaking Children, Carla Wood, Lisa Fitton, Yaacov Petscher, Estrella Rodriguez, Gretchen Sunderman, Taehyeong Lim

Faculty Publications

Purpose: This study aimed to examine the effect of an intensive vocabulary intervention embedded in e-books on the vocabulary skills of young Spanish-English speaking English learners (ELs) from low-socioeconomic status backgrounds. Method: Children (N = 288) in kindergarten and 1st grade were randomly assigned to treatment and read-only conditions. All children received e-book readings approximately 3 times a week for 10-20 weeks using the same books. Children in the treatment condition received e-books supplemented with vocabulary instruction that included scaffolding through explanations in Spanish, repetition in English, checks for understanding, and highlighted morphology. Results: There was a main effect of …

High School Physical Education And Its Effects On Fitness Center Participation After Graduation: A Case Study, David C. Barney, Nathan Kahaialii

Faculty Publications

For those that send off their child into the world, it is hoped they will recall what was taught to them. This applies to high school (HS) physical education (PE) teachers and their students that graduate from HS. The purpose of this case study was to assess current HS PE teachers, fitness club personnel, those that frequent fitness clubs and current HS students regarding HS PE preparing HS students for real world application regarding fitness. Participants from these four groups were interviewed to help better understand the preparation of graduated HS students participating in health clubs. From this case study …

Business Volatility And Employee Performance, Wen-Chyuan Chiang, Li Sun, Brian Walkup

Faculty Publications

This study examines the impact of business volatility on employee performance. We posit that employees work harder in a more volatile business environment as prior research links business volatility to increased use of performance-based compensation systems, which may result in better employee performance. Our results suggest that business volatility has a significant and positive impact on employee performance. Furthermore, we find that the relationship between business volatility and employee performance is stronger for larger firms and firms with higher labor intensity.

Patient-Centered Pain Management Communication From The Patient Perspective, Marie Haverfield, Karleen Giannitrapani, Christine Timko, Karl Lorenz

Faculty Publications

BackgroundPain management discussions between patient and provider can be stressful to navigate and greatly impact the care received. Because of the complexity, emotional color, and sensitivity of pain management, such discussions require a high degree of skill.ObjectiveTo identify patients’ perspectives of patient-centered care communication within the context of pain management discussions.DesignWe conducted semi-structured interviews (25–65 min) with patients regarding their experiences with pain assessment and management.Participants: 36 patients (29 males, 7 females), from 3 Veteran Affairs healthcare locations. Participant age ranged from 28 to 94 with pain intensity ranging from 0 to 10, based on the “pain now” numeric rating …

Electrochemical Glucose Sensors Enhanced By Methyl Viologen And Vertically Aligned Carbon Nanotube Channels, Benjamin J. Brownlee, Meisam Bahari, John N. Harb, Jonathan C. Claussen, Brian D. Iverson

Faculty Publications

Freestanding, vertically aligned carbon nanotubes (VACNTs) were patterned into 16 μm diameter microchannel arrays for flow-through electrochemical glucose sensing. Non-enzymatic sensing of glucose was achieved by the chemical reaction of glucose with methyl viologen (MV) at an elevated temperature and pH (0.1 M NaOH), followed by the electrochemical reaction of reduced-MV with the VACNT surface. The MV sensor required no functionalization (including no metal) and was able to produce on average 3.4 electrons per glucose molecule. The current density of the MV sensor was linear with both flow rate and glucose concentration. Challenges with interference chemicals were mitigated by operating …

Mitochondrial Unfolded Protein Response Gene Clpp Is Required To Maintain Ovarian Follicular Reserve During Aging, For Oocyte Competence, And Development Of Pre-Implantation Embryos, Tianren Wang, Elnur Babayev, Zongliang Jiang, Guangxin Li, Man Zhang, Ecem Esencan, Tamas Horvath, Emre Seli

Faculty Publications

© 2018 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. Caseinolytic peptidase P mediates degradation of unfolded mitochondrial proteins and activates mitochondrial unfolded protein response (mtUPR) to maintain protein homeostasis. Clpp −/− female mice generate a lower number of mature oocytes and two-cell embryos, and no blastocysts. Clpp −/− oocytes have smaller mitochondria, with lower aspect ratio (length/width), and decreased expression of genes that promote fusion. A 4-fold increase in atretic follicles at 3 months, and reduced number of primordial follicles at 6–12 months are observed in Clpp −/− ovaries. This is associated …