Digital Commons Network^™

Faithful Families Cooking And Eating Smart And Moving For Health: Evaluation Of A Community Driven Intervention, Caitlin Torrence, Sarah F. Griffin, Laura Rolke, Kelli Kenison, Altamae Marvin

Faculty Publications

Background: There is an increasing need to adapt and use community interventions to address modifiable behaviors that lead to poor health outcomes, like obesity, diabetes, and heart disease. Poor health outcomes can be tied to community-level factors, such as food deserts and individual behaviors, like sedentary lifestyles, consuming large portion sizes, and eating high-calorie fast food and processed foods. Methods: Through a social ecological approach with family, organization and community, the Faithful Families Cooking and Eating Smart and Moving for Health (FFCESMH) intervention was created to address these concerns in a rural South Carolina community. FFCESMH used gatekeepers to identify …

The Impact Of Peer Support On The Risk Of Future Hospital Readmissions Among Older Adults With A Medical Illness And Co-Occurring Depression, Kyaien O. Conner, Tamara Cadet, Monique J. Brown, Joshua T. Barnett

Faculty Publications

Older adults account for 60% of all preventable hospital readmissions. Although not all readmissions are preventable, evidence indicates that up to 75% of hospital readmissions can be prevented with enhanced patient education, pre-discharge assessment, and effective care upon discharge. Social support, specifically peer support, after discharge from hospital may be a crucial factor in minimizing the risk of preventable hospital readmission. The pilot study reported here evaluated the relationship between peer support and hospital readmissions in a sample of depressed older adults (N = 41) who were recently discharged from hospital due to a medical condition and who simultaneously had …

Effects Of Protein Supplementation On Performance And Recovery In Resistance And Endurance Training, Harry P. Cintineo, Michelle A. Arent, Jose Antonio, Shawn M. Arent Ph.D., Cscs*D, Fissn, Facsm, Fnak

Faculty Publications

There is robust evidence which shows that consuming protein pre- and/or post-workout induces a significant rise in muscle protein synthesis. It should be noted, however, that total daily caloric and protein intake over the long term play the most crucial dietary roles in facilitating adaptations to exercise. However, once these factors are accounted for, it appears that peri-exercise protein intake, particularly in the post-training period, plays a potentially useful role in terms of optimizing physical performance and positively influencing the subsequent recovery processes for both resistance training and endurance exercise. Factors that affect the utility of pre- or post-workout feeding …

Edwardsiella Ictaluri Type Iii Secretion System (T3ss) Effector Esen Is A Phosphothreonine Lyase That Inactivates Erk1/2, Lidiya P. Dubytska, Ronald L. Thune

Faculty Publications

© The authors 2018. EseN is a type III secretion system (T3SS) effector that is encoded on the Edwardsiella ictaluri chromosome and is homologous to a family of T3SS effector proteins with phosphothreonine lyase (PTL) activity, including OspF from Shigella and SpvC from Salmonella. A yeast-2-hybrid system was used to identify the major vault protein (MVP) as a specific host-cell binding partner for EseN, and the proximity ligation assay (PLA) confirmed the interaction. Similar to other pathogens, E. ictaluri invasion activates extracellular signal-regulated kinases 1 and 2 (ERK1/2) early in the infection, which are subsequently inactivated by EseN. Structurally, EseN …

Ubiquitin Receptor Rpn13 Mediates The Inhibitory Interaction Of Diphenyldihaloketones Clefma And Ef24 With The 26s Proteasome, Geeta Rao, Gregory Nkepang, Jian Xu, Hooman Yari, Hailey Houson, Chengwen Teng Pharm.D., Ph.D., Vibhudutta Awasthi

Faculty Publications

The proteasome is a validated target in drug discovery for diseases associated with unusual proteasomal activity. Here we report that two diphenyldihaloketones, CLEFMA and EF24, inhibit the peptidase activity of the 26S proteasome. The objective of this study was to investigate interaction of these compounds with the proteasome and identify a putative target within the protein components of the 26S proteasome. We employed standard fluorogenic peptide-based proteasome activity assay for trypsin-like, chymotrypsin-like, and caspase-like activities of human purified 26S proteasome in cell-free conditions. GFPu-1 and HUVEC cells were used as proteasome reporter cells. Direct binding studies used purified 19S, 20S, …

Toxoplasma Gondii Seropositivity And Substance Use In Us Adults, Bruce L. Brown, Andrew N. Berrett, Shawn D. Gale, Lance D. Erickson, Evan L. Thacker, Dawson W. Hedges

Faculty Publications

The intracellular parasite Toxoplasma gondii (Nicolle et Manceaux, 1908) infects humans resulting in acute toxoplasmosis, an infection that in immunocompetent people is typically mild but results in persistent latent toxoplasmosis. In that T. gondii appears to affect dopamine synthesis and because addicting drugs affect midbrain dopamine transmission, latent toxoplasmosis could influence substance use. Using both the third and continuous National Health and Nutrition Examination Surveys from the US Centers for Disease Control and Prevention, we used logistic regression to test for associations between T. gondii seropositivity and subject self-report of having ever used tobacco, alcohol, marijuana, cocaine, heroin, or methamphetamine. …

End-To-End Convolutional Neural Network Model For Gear Fault Diagnosis Based On Sound Signals, Yong Yao, Honglei Wang, Shaobo Li, Zhongnhao Liu, Gui Gui, Yabo Dan, Jianjun Hu

Faculty Publications

Currently gear fault diagnosis is mainly based on vibration signals with a few studies on acoustic signal analysis. However, vibration signal acquisition is limited by its contact measuring while traditional acoustic-based gear fault diagnosis relies heavily on prior knowledge of signal processing techniques and diagnostic expertise. In this paper, a novel deep learning-based gear fault diagnosis method is proposed based on sound signal analysis. By establishing an end-to-end convolutional neural network (CNN), the time and frequency domain signals can be fed into the model as raw signals without feature engineering. Moreover, multi-channel information from different microphones can also be fused …

Spatial Distribution And Contamination Assessment Of Surface Heavy Metals Off The Western Guangdong Province And Northeastern Hainan Island, Qian Ge, Zuo George Xue, Fengyou Chu

Faculty Publications

Surface sediments collected from the continental shelf off the western Guangdong Province and northeastern Hainan Island are analyzed for selected heavy metals contents including Cd, Cr, Cu, Pb, Zn, and As to determine spatial distribution, potential ecological risks, and sources. In addition, some of the controlling factors of heavy metals distribution are also discussed. The average heavy metals contents decrease in the order of Zn > Cr > Pb > Cu > As > Cd. The averaged pollution degree, as shown by the index of geo-accumulation (Igeo), decreases in the order of Zn > Cu > Pb > Cr > Cd > As. Due to the barrier of islands, the …

Barriers And Facilitators To Compliance With A State Healthy Eating Policy In Early Care And Education Centers, Daniel A. Zaltz, Russell R. Pate, Jennifer R. O'Neill, Brian Neelon, Sara E. Benjamin-Neelon

Faculty Publications

Background: Early care and education (ECE) policies can improve childhood obesity risk factors. We evaluated barriers and facilitators to implementing mandatory nutrition standards for foods provided in South Carolina ECE centers serving lowincome children, comparing centers participating in the Child and Adult Care Food Program (CACFP) with non-CACFP centers.

Methods: We mailed 261 surveys (demographics, policies and practices, barriers and facilitators) to center directors after new state nutrition standards were implemented in South Carolina. We conducted univariate and bivariate analyses to explore relationships between barriers, facilitators, and center-level characteristics, by CACFP status.

Results: We received 163 surveys (62% response rate). …

Gene Regulation By Redox-Sensitive Burkholderia Thailandensis Ohrr And Its Role In Bacterial Killing Of Caenorhabditis Elegans, Anuja Pande, Todd C. Veale, Anne Grove

Faculty Publications

© 2018 American Society for Microbiology. Fatty acid hydroperoxides are involved in host-pathogen interactions. In both plants and mammals, polyunsaturated fatty acids are liberated during infection and enzymatically oxidized to the corresponding toxic hydroperoxides during the defensive oxidative burst that is designed to thwart the infection. The bacterial transcription factor OhrR (organic hydroperoxide reductase regulator) is oxidized by organic hydroperoxides, as a result of which the ohr gene encoding organic hydroperoxide reductase is induced. This enzyme converts the hydroperoxides to less toxic alcohols. We show here that OhrR from Burkholderia thailandensis represses expression of ohr. Gene expression is induced by …

Circular Rna Identified From Peg3 And Igf2r, Bambarendage P.U. Perera, Subash Ghimire, Joomyeong Kim

Faculty Publications

Copyright: © 2018 Perera et al. Circular RNA is a newly discovered class of non-coding RNA generated through the back-splicing of linear pre-mRNA. In the current study, we characterized two circular RNAs that had been identified through NGS-based 5'RACE experiments. According to the results, the Peg3 locus contains a 214-nucleotide-long circular RNA, circPeg3, that is detected in low abundance from the neonatal brain, lung and ovary. In contrast, the Igf2r locus contains a group of highly abundant circular RNAs, circIgf2r, showing multiple forms with various exon combinations. In both cases, the expression patterns of circPeg3 and circIgf2r among individual tissues …

4c-Seq Characterization Of Drosophila Beaf Binding Regions Provides Evidence For Highly Variable Long-Distance Interactions Between Active Chromatin, Shraddha Shrestha, Dong Ha Oh, J. Keller Mckowen, Maheshi Dassanayake, Craig M. Hart

Faculty Publications

© 2018 Shrestha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Chromatin organization is crucial for nuclear functions such as gene regulation, DNA replication and DNA repair. Insulator binding proteins, such as the Drosophila Boundary Element-Associated Factor (BEAF), are involved in chromatin organization. To further understand the role of BEAF, we detected cis-and trans-interaction partners of four BEAF binding regions (viewpoints) using 4C (circular chromosome conformation capture) and analyzed their association with …

Relief Of The Dma1-Mediated Checkpoint Requires Dma1 Autoubiquitination And Dynamic Localization, Christine M. Jones, Jun Song Chen, Alyssa E. Johnson, Zachary C. Elmore, Sierra N. Cullati, Janel R. Beckley, Kathleen L. Gould

Faculty Publications

© 2018 Jones, Chen, et al. Chromosome segregation and cell division are coupled to prevent aneuploidy and cell death. In the fission yeast Schizosaccharomyces pombe, the septation initiation network (SIN) promotes cytokinesis, but upon mitotic checkpoint activation, the SIN is actively inhibited to prevent cytokinesis from occurring before chromosomes have safely segregated. SIN inhibition during the mitotic checkpoint is mediated by the E3 ubiquitin ligase Dma1. Dma1 binds to the CK1-phosphorylated SIN scaffold protein Sid4 at the spindle pole body (SPB), and ubiquitinates it. Sid4 ubiquitination antagonizes the SPB localization of the Polo-like kinase Plo1, the major SIN activator, so …

Adipose Tissue Dysfunction Occurs Independently Of Obesity In Adipocyte-Specific Oncostatin Receptor Knockout Mice, Jacqueline M. Stephens, Jennifer L. Bailey, Hardy Hang, Victoria Rittell, Marilyn A. Dietrich, Randall L. Mynatt, Carrie M. Elks

Faculty Publications

© 2018 The Obesity Society Objective: This study examined the phenotypic effects of adipocyte-specific oncostatin M receptor (OSMR) loss in chow-fed mice. Methods: Chow-fed adipocyte-specific OSMR knockout (FKO) mice and littermate OSMRfl/fl controls were studied. Tissue weights, insulin sensitivity, adipokine production, and stromal cell immunophenotypes were assessed in epididymal fat (eWAT); serum adipokine production was also assessed. In vitro, adipocytes were treated with oncostatin M, and adipokine gene expression was assessed. Results: Body weights, fasting blood glucose levels, and eWAT weights did not differ between genotypes. However, the eWAT of OSMRFKO mice was modestly less responsive to insulin stimulation than …

New Mouse Model Of Pulmonary Hypertension Induced By Respiratory Syncytial Virus Bronchiolitis, Dai Kimura, Jordy Saravia, Sridhar Jaligama, Isabella Mcnamara, Luan D. Vu, Ryan D. Sullivan, Salvatore Mancarella, Dahui You, Stephania A. Cormier

Faculty Publications

© 2018 American Physiological Society. All rights reserved. Pulmonary hypertension (PH) has been observed in up to 75% of infants with moderate to severe respiratory syncytial virus (RSV) bronchiolitis and is associated with significant morbidity and mortality in infants with congenital heart disease. The purpose of the present study was to establish a mouse model of PH secondary to RSV bronchiolitis that mimics the disease etiology as it occurs in infants. Neonatal mice were infected with RSV at 5 days of age and then reinfected 4 wk later. Serum-free medium was administered to age-matched mice as a control. Echocardiography and …

Dispersal Records Of The Sugarcane Aphid, Melanaphis Sacchari (Zehntner) (Hemiptera: Aphididae), Through The Midwest Suction Trap Network, Doris Lagos-Kutz, David Voegtlin, Jeffrey Davis, Glen Hartman

Faculty Publications

Summary Melanaphis sacchari (Zehntner) (Hemiptera: Aphididae), the sugarcane aphid, is an invasive species in the United States. This species is a threat to crops of economic importance including sorghum and sugar cane. Therefore, monitoring dispersal of this pest is necessary to document its geographic expansion. Based on trap catches obtained from the Midwest Suction Trap Network during 2015 to 2017, sugarcane aphids arrived late in the growing season as far as northern Wisconsin. We will continue to monitor the dispersal of this aphid species through the Midwest Suction Trap Network in order to provide information to researchers, extension agents, and …

Production Of Live Young With Cryopreserved Sperm From The Endangered Livebearing Fish Redtail Splitfin (Xenotoca Eiseni, Rutter, 1896), Yue Liu, Harry Grier, Terrence R. Tiersch

Faculty Publications

Previous studies of sperm cryopreservation of livebearing fish have been limited to two genera within the family Poeciliidae. The goal of the present study was to investigate the feasibility to produce live young of livebearing goodeids (family Goodeidae) with cryopreserved sperm, using aquarium-trade populations of the endangered species Redtail Splitfin (Xenotoca eiseni, Rutter, 1896). Reproductive condition of females was evaluated by histological categorization of ovarian development. A total of 117 females were inseminated with cryopreserved sperm, 81 were inseminated with fresh sperm, 27 were mixed with males for natural breeding, and 30 were maintained without males or insemination. Histological images …

Effect Of 12 Wk Of Resistant Starch Supplementation On Cardiometabolic Risk Factors In Adults With Prediabetes: A Randomized Controlled Trial, Courtney M. Peterson, Robbie A. Beyl, Kara L. Marlatt, Corby K. Martin, Kayanush J. Aryana, Maria L. Marco, Roy J. Martin, Michael J. Keenan, Eric Ravussin

Faculty Publications

Background: Type 2 resistant starch (RS2) has been shown to improve glycemic control and some cardiovascular endpoints in rodent and human studies. Objective: The aim of this study was to perform one of the first randomized clinical trials in adults with prediabetes and one of the longest trials to test whether RS2 can improve cardiometabolic health. Design: 68 overweight [body mass index (BMI) ≥27 kg/m2] adults aged 35-75 y with prediabetes were randomized to consume 45 g/d of high-amylose maize (RS2) or an isocaloric amount of the rapidly digestible starch amylopectin (control) for 12 wk. At baseline and postintervention, ectopic …

Exploring The Impact Of Efficacy Messages On Cessation-Related Outcomes Using Ecological Momentary Assessment, Victoria Lambert, Stuart G. Ferguson, Jeff Niederdeppe, David Hammond, James W. Hardin, Jim Thrasher

Faculty Publications

Introduction:
Observational studies indicate that cigarette package inserts with efficacy messages about the benefits of quitting (i.e. response efficacy) and recommendations for successful cessation increase smokers’ self-efficacy to quit and promote sustained cessation. However, the effects of inserts with such efficacy messages have not been studied using experimental designs. This study used ecological momentary assessment (EMA) to assess smokers’ responses to efficacy inserts.

Material and Methods:
In a randomized case-crossover study among smokers from the United States (n=23), participants received a one-week supply of cigarettes with efficacy inserts and a one-week supply without any inserts, and were randomized to use …

Managing Dissent, Timothy Zick

Faculty Publications

In his insightful new book, Managed Speech: The Roberts Court's First Amendment (2017), Professor Greg Magarian criticizes the Roberts Court for adopting a "managed speech" approach in its First Amendment cases. According to Professor Magarian, that approach gives too much power to private and governmental actors to manage public discourse, constrain dissident speakers, and instill social and political stability. This Article argues that at least insofar as it relates to many forms of public dissent, the managed speech approach is both deeply rooted in First Amendment jurisprudence and culturally prevalent. Historically, First Amendment jurisprudence has expressed support for narrowly managed …

Motherhood, Abortion, And The Medicalization Of Women’S Poverty, Michelle Oberman

Faculty Publications

This article considers the impact of laws and policies that determine who experiences unplanned pregnancy, who has abortions, and how economic status shapes one’s response to unplanned pregnancy. There is a well-documented correlation between abortion and poverty: poor women have more abortions than do their richer sisters. Equally well-documented is the correlation between unplanned pregnancy and poverty. Finally, the high cost of motherhood for poor women and their offspring manifests in disproportionately high lifelong rates of poverty, ill-health and mortality for offspring and mothers, alike. Read together, these factors offer a vivid illustration of the medicalization of poverty.

Teaching Population Health: Innovations In The Integration Of The Healthcare And Public Health Systems, Rosemary M. Caron, Anne M. Hewitt, Julie H. Carmalt, Edmond A. Hooker

Faculty Publications

Population health is a critical concept in healthcare delivery today. Many healthcare administrators are struggling to adapt their organization from fee-for-service to value delivery. Payers and patients expect healthcare leaders to understand how to deliver care under this new model. Health administration programs play a critical role in training future leaders of healthcare organizations to be adaptable and effective in this dynamic environment. The purpose of this research was to: (a) engage current educators of health administration students in a dialogue about the best practices of integrating the healthcare and public health systems; (b) identify the content and pedagogy for …

Pedagogy: How To Best Teach Population Health To Future Healthcare Leaders, Rosemary M. Caron, Edmond A. Hooker, Anne M. Hewitt, Julie H. Carmalt

Faculty Publications

Our healthcare system is moving from a fee-for-service reimbursement model to one that provides payment for improvements in three areas related to care: quality, coordination, and cost. Healthcare organizations must use a population health approach when delivering care under this new paradigm. Health administration programs play a critical role in training future leaders of healthcare organizations to be adaptable and effective in this dynamic environment. The purpose of this research was to: (1) engage health administration educators in a dialogue about population health and its relevance to healthcare administration education; (2) describe pedagogical methods appropriate for teaching population health skills …

Cause And Consequence Of Aβ: Lipid Interactions In Alzheimer Disease Pathogenesis, Vijay Rangachari, Dexter N. Dean, Pratip Rana, Ashwin Vaidya, Preetam Ghosh

Faculty Publications

Self-templating propagation of protein aggregate conformations is increasingly becoming a significant factor in many neurological diseases. In Alzheimer disease (AD), intrinsically disordered amyloid-β (Aβ) peptides undergo aggregation that is sensitive to environmental conditions. High-molecular weight aggregates of Aβ that form insoluble fibrils are deposited as senile plaques in AD brains. However, low-molecular weight aggregates called soluble oligomers are known to be the primary toxic agents responsible for neuronal dysfunction. The aggregation process is highly stochastic involving both homotypic (Aβ-Aβ) and heterotypic (Aβ with interacting partners) interactions. Two of the important members of interacting partners are membrane lipids and surfactants, to …

Challenges, Pitfalls And Surprises: Development And Validation Of A Monoclonal Antibody For Enzyme Immunoassay Of The Steroid 1Α-Hydroxycorticosterone In Elasmobranch Species, Catharine J. Wheaton, Natalie D. Mylniczenko, John M. Rimoldi, Rama S.V.S. Gadepalli, R. Hart, Bobbi R. O'Hara, Andrew N. Evans

Faculty Publications

Sharks and rays are popular species used in wildlife ecotourism and aquariums to educate the public on the behavior, ecology and conservation challenges of elasmobranchs. To understand long-term physiological health and welfare under varying social and husbandry conditions, we developed and validated an enzyme immunoassay (EIA) to measure stress/ionoregulatory hormones in managed and semi-free range southern rays (Hypanus americanus). Banked serum and interrenal samples from 27 female rays managed at Disney’s The Seas with Nemo and Friends^® and Castaway Cay were used to evaluate measurement of 1α-hydroxycorticosterone (1αOHB) relative to corticosterone (B). Although commercial EIAs are available …

Influenza Virus Infectivity Is Retained In Aerosols And Droplets Independent Of Relative Humidity, Karen A. Kormuth, Kaisen Lin, Aaron J. Prussin, Eric P. Vejerano, Andrea J. Tiwari, Steve S. Cox, Micheal M. Myerburg, Seema S. Lakdawala, Linsey C. Marr

Faculty Publications

Pandemic and seasonal influenza viruses can be transmitted through aerosols and droplets, in which viruses must remain stable and infectious across a wide range of environmental conditions. Using humidity-controlled chambers, we studied the impact of relative humidity on the stability of 2009 pandemic influenza A(H1N1) virus in suspended aerosols and stationary droplets. Contrary to the prevailing paradigm that humidity modulates the stability of respiratory viruses in aerosols, we found that viruses supplemented with material from the apical surface of differentiated primary human airway epithelial cells remained equally infectious for 1 hour at all relative humidities tested. This sustained infectivity was …

Makerspaces For All: Serving Lgbtq Makers In School Libraries, Vanessa Kitzie, Healther Moorefield-Lang

Faculty Publications

The article discusses makerspace that is defined by Laura Fleming as a metaphor for a unique learning environment that encourages tinkering, play, and open-ended exploration for all. It mentions that makerspaces are considered safe spaces for LGBTQ youth as they can create products that establish and communicate their LGBTQ identities. Also discussed is LGBTQ students perception regarding libraries.

Agencies, Courts, First Principles, And The Rule Of Law, Todd S. Aagaard

Faculty Publications

An inherent tension exists between the roles of agencies and courts in administrative enforcement cases. Those cases are initiated by agencies to enforce the statutes and regulations they administer but are then subject to judicial review by courts. Agencies tend to focus on creating and then implementing administrative enforcement systems that produce consistent results pursuant to the agency’s bureaucratic processes and informed by the agency’s expertise. In reviewing such decisions, by contrast, courts tend to focus on ensuring that the agency’s administrative system comports with fundamental legal principles. The two roles embody different goals: for agencies, rendering predictable and consistent …

Sex Differences In Rhesus Monkeys’ Digit Ratio (2d:4d Ratio) And Its Association With Maternal Social Dominance Rank, Alexander Baxter, Elizabeth K. Wood, Parker Jarman, Ashley N. Cameron, John P. Capitanio, James Dee Higley

Faculty Publications

Prenatal androgen exposure (PAE) plays a pivotal role in masculinizing the developing body and brain, and extreme exposure may contribute to autism, anxiety disorder and schizophrenia. One commonly used biomarker for PAE is the pointer-to-ring-finger digit length (2D:4D) ratio. Although this biomarker is widely used in human studies, relatively few studies have investigated 2D:4D ratio in nonhuman primates, particularly rhesus macaques (Macaca mulatta), one of the most commonly used animals in biomedical research. Thus far, data suggest that sexual dimorphism in 2D:4D ratio may be in the opposite direction in some monkey species, when compared to the pattern …

Advancing Social Connection As A Public Health Priority In The United States, Julianne Holt-Lunstad, Theodore Robles, David A. Sbarra

Faculty Publications

A robust body of scientific evidence indicates that being embedded in high-quality close relationships and feeling socially connected to the people in your life is associated with decreased risk for all-cause mortality as well as a range of disease morbidities. Despite mounting evidence that the magnitude of these associations is comparable to many leading health determinants (that receive significant public health resources), government agencies, healthcare providers and associations, and public/private healthcare funders are slow to recognize human social relationships as either a health determinant or health risk marker in a manner that is comparable to other public health priorities. This …