Digital Commons Network^™

Repeated Vaginal Exposures To The Common Cosmetic And Household Preservative Methylisothiazolinone Induce Persistent, Mast Cell-Dependent Genital Pain In Nd4 Mice, Erica Arriaga-Gomez, Jacyln Kline, Elizabeth Emanuel, Nefeli Neamonitaki, Tenzin Yangdon, Hayley Zacheis, Dogukan Pasha, Jinyoung Lim, Susan Bush, Beebie Boo, Hanna Mengistu, Ruby Kinnamon, Robin Shields-Cutler, Wattenberg Wattenberg, Devavani Chatterjea

Faculty Publications

A history of allergies doubles the risk of vulvodynia-a chronic pain condition of unknown etiology often accompanied by increases in numbers of vulvar mast cells. We previously established the biological plausibility of this relationship in mouse models where repeated exposures to the allergens oxazolone or dinitrofluorobenzene on the labiar skin or inside the vaginal canal of ND4 Swiss Webster outbred mice led to persistent tactile sensitivity and local increases in mast cells. In these models, depletion of mast cells alleviated pain. While exposure to cleaning chemicals has been connected to elevated vulvodynia risk, no single agent has been linked to …

The Association Between Dietary Inflammatory Index (Dii) And Cancer Risk In Korea: A Prospective Cohort Study Within The Koges-Hexa Study, Injeong Ryu, Minji Kwon, Cheongmin Sohn, Nitin Shivappa, James R. Hébert, Woori Na, Mi Kyung Kim

Faculty Publications

Several epidemiological studies have shown that there are consistently positive associations between dietary inflammatory index (DII (R)) scores and cancer incidence in Western populations. However, few DII-cancer studies have been conducted in East Asian populations. In a large cohort representative of the general Korean population, we investigated whether the DII is associated with overall cancer risk. A total of 163,660 participants (56,781 males and 106,879 females) had evaluable data for analyses. This follow-up study was carried out over the course of 7.9 years. DII scores were calculated based on Semi-Quantitative Food-Frequency Questionnaire (SQ-FFQ) data for 106 food items. Cancers were …

An Open-Source Tool To Facilitate The Processing Of Grace Observations And Gldas Outputs: An Evaluation In Bangladesh, Adam J. Purdy, Cédric H. David, Md. Safat Sikder, John T. Reager, Hrishikesh A. Chandanpukar, Norman L. Jones, Mir A. Matin

Faculty Publications

Bangladesh lies at the intersection of the Ganges, Brahmaputra, and Meghna rivers with a combined average discharge of 38,000 m3s−1 ranking fourth globally. Despite the volume of water flowing through and seasonally inundating parts of the landscape, groundwater reliance is necessary to support an intensive agricultural industry. Here we use newly-developed open-source software to combine observations from the Gravity Recovery and Climate Experiment (GRACE) satellites with hydrologic estimates of land water storage from the Global Land Assimilation Data System (GLDAS) to isolate basin-scale groundwater anomalies in Northwest Bangladesh from 2002 to 2016. We place our estimates in the context of …

Purification Of Tag-Free Chlamydia Trachomatis Scc4 For Structural Studies Using Sarkosyl-Assisted On-Column Complex Dissociation, Thilini O. Ukwaththage, Octavia Y. Goodwin, Abigael C. Songok, Alexa M. Tafaro, Li Shen, Megan A. Macnaughtan

Faculty Publications

Copyright © 2019 American Chemical Society. Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes the most common sexually transmitted bacterial disease in the world. The bacterium has a unique biphasic developmental cycle with a type III secretion system (T3SS) to invade host cells. Scc4 is a class I T3SS chaperone forming a heterodimer complex with Scc1 to chaperone the essential virulence effector, CopN. Scc4 also functions as an RNA polymerase binding protein to regulate σ66-dependent transcription. Aggregation and low solubility of 6X-histidine-tagged Scc4 and the insolubility of 6X-histidine and FLAG-tagged Scc1 expressed in Escherichia coli have hindered the …

Gut Dna Virome Diversity And Its Association With Host Bacteria Regulate Inflammatory Phenotype And Neuronal Immunotoxicity In Experimental Gulf War Illness, Ratanesh K. Seth, Rabia Maqsood, Ayan Mondal, Dipro Bose, Diana Kimono, Larinda A. Holland, Patricia Janulewicz Lloyd, Nancy Klimas, Ronnie Horner, Kimberly Sullivan, Efrem S. Lim, Saurabh Chatterjee

Faculty Publications

Gulf War illness (GWI) is characterized by the persistence of inflammatory bowel disease, chronic fatigue, neuroinflammation, headache, cognitive impairment, and other medically unexplained conditions. Results using a murine model show that enteric viral populations especially bacteriophages were altered in GWI. The increased viral richness and alpha diversity correlated positively with gut bacterial dysbiosis and proinflammatory cytokines. Altered virome signature in GWI mice also had a concomitant weakening of intestinal epithelial tight junctions with a significant increase in Claudin-2 protein expression and decrease in ZO1 and Occludin mRNA expression. The altered virome signature in GWI, decreased tight junction protein level was …

Considering "Atmosphere" When Facilitating Information Seeking By People With Invisible Disabilities In Public Libraries, Rebecca Muir, Kim M. Thompson, Asim Qayyum

Faculty Publications

Twenty percent of Australians reported having a disability in 2015. Disability may occur at any time during the lifespan, however most disabilities are invisible. When a disability is invisible, or not immediately apparent to an outsider, individuals may need to self-identify to access inclusive services, or accommodation may never be offered at all.

When the perceived number of information seekers with a disability is low, information organisations may deem services unnecessary. Considering information access is a human right, information service providers and researchers need to seek low cost and low effort ways to facilitate information access and information seeking behaviors. …

Superior Colliculus Lesions Lead To Disrupted Responses To Light In Diurnal Grass Rats (Arvicanthis Niloticus), Andrew J. Gall, Alyssa M. Goodwin, Ohanes S. Khacherian, Laura B. Teal

Faculty Publications

The circadian system regulates daily rhythms of physiology and behavior. Although extraordinary advances have been made to elucidate the brain mechanisms underlying the circadian system in nocturnal species, less is known in diurnal species. Recent studies have shown that retinorecipient brain areas such as the intergeniculate leaflet (IGL) and olivary pretectal nucleus (OPT) are critical for the display of normal patterns of daily activity in diurnal grass rats (Arvicanthis niloticus). Specifically, grass rats with IGL and OPT lesions respond to light in similar ways to intact nocturnal animals. Importantly, both the IGL and OPT project to one another …

Understanding And Use Of Food Labeling Systems Among Whites And Latinos In The United States And Among Mexicans: Results From The International Food Policy Study, 2017, Claudia Nieto, Alejandra Jauregui, Alejandra Contreras-Manzano, Edna Arillo-Santillan, Simon Barquera, Christine M. White, David Hammond, Jim Thrasher

Faculty Publications

Background Obesity and chronic diseases could be prevented through improved diet. Most governments require at least one type of food labeling system on packaged foods to communicate nutrition information and promote healthy eating. This study evaluated adult consumer understanding and use of nutrition labeling systems in the US and Mexico, the most obese countries in the world.

Methods Adults from online consumer panels in the US (Whites n = 2959; Latinos n = 667) and in Mexico (n = 3533) were shown five food labeling systems: 1. Nutrition Facts Table (NFT) that shows nutrients of concern per serving; 2. Guideline …

Circulating Obesity-Driven Biomarkers Are Associated With Risk Of Clear Cell Renal Cell Carcinoma: A Two-Stage, Case-Control Study, Qinchuan Wang, Huakang Tu, Meiling Zhu, Dong Liang, Yuanqing Ye, David W. Chang, Yin Long, Xifeng Wu

Faculty Publications

Obesity is one of modifiable risk factors for clear cell renal cell cancer (ccRCC). We aim to identify the association between obesity-driven biomarkers and ccRCC risk. This is a retrospective, two-phase, case-control study involving 682 cases and 733 controls. Obesity-driven biomarkers [gastric inhibitory polypeptide (GIP), C-peptide, insulin, resistin, adipsin, peptide YY, pancreatic polypeptide, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), plasminogen activator inhibitor-1, monocyte chemoattractant protein 1, lipocalin2, leptin, adiponectin] were measured using the Milliplex method. Multivariate logistic regression was used to assess the associations between biomarkers and ccRCC risk. Results revealed that GIP, C-peptide, IL-6 and TNF-α levels were consistently …

Current Understanding Of West Nile Virus Clinical Manifestations, Immune Responses, Neuroinvasion, And Immunotherapeutic Implications, Fengwei Bai, E. Ashley Thompson, Parminder J.S. Vig, A. Arturo Leis

Faculty Publications

West Nile virus (WNV) is the most common mosquito-borne virus in North America. WNV-associated neuroinvasive disease affects all ages, although elderly and immunocompromised individuals are particularly at risk. WNV neuroinvasive disease has killed over 2300 Americans since WNV entered into the United States in the New York City outbreak of 1999. Despite 20 years of intensive laboratory and clinical research, there are still no approved vaccines or antivirals available for human use. However, rapid progress has been made in both understanding the pathogenesis of WNV and treatment in clinical practices. This review summarizes our current understanding of WNV infection in …

Short Term Effects Of Hurricane Irma And Cyanobacterial Blooms On Ammonium Cycling Along A Freshwater-Estuarine Continuum In South Florida, Justyna J. Hampel, Mark J. Mccarthy, Megan H. Reed, Silvia E. Newell

Faculty Publications

Lacustrine and coastal systems are vulnerable to the increasing number and intensity of tropical storms driven by climate change. Strong winds associated with tropical storms can mobilize nutrients in sediments and alter nitrogen and phosphorus cycling, leading to amplification of preexisting conditions, such as eutrophication and cyanobacterial blooms (cyanoHABs). In 2016, Florida declared a State of Emergency within and downstream of Lake Okeechobee (LO) due to toxic cyanobacterial blooms (primarily Microcystis). The blooms originated in LO, but flood control measures released water from LO to the brackish St. Lucie Estuary (SLE). In September 2017, Hurricane Irma traversed the Florida …

Longitudinal Study Of Externalizing Behaviors In Latino/A Adolescents: An Examination Of Parenting And Educational Factors, Sergio B. Pereyra, Roy A. Bean, Jeremy B. Yorgason, Chien-Ti Lee, Jonathan G. Sandberg, Richard B. Miller

Faculty Publications

As the Latino/a population increases externalizing behaviors among adolescents continue to concern researchers and clinicians. National Longitudinal Study of Adolescent Health (Add Health) data were used to analyze direct and indirect effects of parenting and academic factors on externalizing behavior among Latino/a adolescents over time, using latent growth curves and structural equation modeling (SEM). Results indicated that higher levels of maternal warmth, the adolescent-teacher relationship, and academic achievement were all negatively associated with initial levels of externalizing behavior and in some cases negatively predicted the rate of change of externalizing behavior. Some predictors were also all found to be positively …

Environmentally Persistent Free Radicals: Linking Air Pollution And Poor Respiratory Health?, Peter D. Sly, Stephania A. Cormier, Slawomir Lomnicki, Jeffrey N. Harding, Keith Grimwood, Griffith University

Faculty Publications

No abstract provided.

Dysbiosis-Associated Enteric Glial Cell Immune-Activation And Redox Imbalance Modulate Tight Junction Protein Expression In Gulf War Illness Pathology, Diana Kimono, Sutapa Sarkar, Muayad Albadrani, Ratanesh K. Seth, Dipro Bose, Ayan Mondal, Yuxi Li, Amar N. Kar, Mitzi Nagarkatti, Prakash Nagarkatti, Kimberly Sullivan, Patricia Janulewicz, Stephen Lasley, Ronnie Horner, Nancy Klimas, Saurabh Chatterjee

Faculty Publications

About 14% of veterans who suffer from Gulf war illness (GWI) complain of some form of gastrointestinal disorder but with no significant markers of clinical pathology. Our previous studies have shown that exposure to GW chemicals resulted in altered microbiome which was associated with damage associated molecular pattern (DAMP) release followed by neuro and gastrointestinal inflammation with loss of gut barrier integrity. Enteric glial cells (EGC) are emerging as important regulators of the gastrointestinal tract and have been observed to change to a reactive phenotype in several functional gastrointestinal disorders such as IBS and IBD. This study is aimed at …

Parasitic Microbiome Project: Grand Challenges, Nolwenn M. Dheilly, Joaquín Martínez Martínez, Karyna Rosario, Paul J. Brindley, Raina N. Fichorava, Jonathan Z. Kaye, Kevin D. Kohl, Laura J. Knoll, Julius Lukeš, Susan L. Perkins, Robert Poulin, Lynn Schriml, Luke R. Thompson

Faculty Publications

No abstract provided.

Effect Of Biochar Amendment On Sorption-Desorption And Dissipation Of 17Α‑Ethinylestradiol In Sandy Loam And Clay Soils, Zhuo Wei, Jim J. Wang, Amy B. Hernandez, Andrea Warren, Jong Hwan Park, Yili Meng, Syam K. Dodla, Changyoon Jeong

Faculty Publications

Animal manure application in agricultural land has caused the release of steroid estrogens in the soil environment and further movement to aquatic systems. The objective of this study was to investigate the effects of biochar addition on sorption-desorption and dissipation behaviors of 17α‑ethinylestradiol (EE2) in two different textured soils. A Commerce sandy loam and a Shakey clay were selected and subjected to sterilization. Soil samples with and without sterilization were reacted with a series of EE2 solutions of different concentrations for sorption followed by desorption and quantification using HPLC-MS/MS. Long-term dissipation of EE2 in the same soils was also evaluated …

Does Time Spent Using Social Media Impact Mental Health?: An Eight Year Longitudinal Study, Sarah M. Coyne, Adam A. Rogers, Jessica D. Zurcher, Laura Stockdale, Mccall Booth

Faculty Publications

Many studies have found a link between time spent using social media and mental health issues, such as depression and anxiety. However, the existing research is plagued by cross-sectional research and lacks analytic techniques examining individual change over time. The current research involves an 8-year longitudinal study examining the association between time spent using social media and depression and anxiety at the intra-individual level. Participants included 500 adolescents who completed once-yearly questionnaires between the ages of 13 and 20. Results revealed that increased time spent on social media was not associated with increased mental health issues across development when examined …

Reliability Of The American Community Survey Estimates Of Risk-Adjusted Readmission Rankings For Hospitals Before And After Peer Group Stratification, Nathaniel Bell, Ana Lòpez-De Fede, Bo Cai

Faculty Publications

Importance Since the transition to the American Community Survey, data uncertainty has complicated its use for policy making and research, despite the ongoing need to identify disparities in health care outcomes. The US Centers for Medicare & Medicaid Services’ new, stratified payment adjustment method for its Hospital Readmissions Reduction Program may be able to reduce the reliance on data linkages to socioeconomic survey estimates.

Objective To determine whether there are differences in the reliability of socioeconomically risk-adjusted hospital readmission rates among hospitals that serve a disproportionate share of low-income populations after stratifying hospitals into peer group–based classification groups.

Design, Setting, …

Detecting Parasite Associations Within Multi-Species Host And Parasite Communities, Tad A. Dallas, Anna Liisa Laine, Otso Ovaskainen

Faculty Publications

© 2019 The Author(s) Published by the Royal Society. All rights reserved. Understanding the role of biotic interactions in shaping natural communities is a long-standing challenge in ecology. It is particularly pertinent to parasite communities sharing the same host communities and individuals, as the interactions among parasites—both competition and facilitation—may have far-reaching implications for parasite transmission and evolution. Aggregated parasite burdens may suggest that infected host individuals are either more prone to infection, or that infection by a parasite species facilitates another, leading to a positive parasite–parasite interaction. However, parasite species may also compete for host resources, leading to the …

Enabling Stakeholder Decision-Making With Earth Observation And Modeling Data Using Tethys Platform, E. James Nelson, Sarva T. Pulla, Mir A. Matin, Kiran Shakya, Norm Jones, Daniel P. Ames, W. Lee Ellenburg, Kel N. Markert, Cédric H. David, Benjamin F. Zaitchik, Patrick Gatlin, Riley Hales

Faculty Publications

Tethys Platform is an open source framework for developing web-based applications for Earth Observation data. Our experience shows that Tethys significantly lowers the barrier for cloud-based app development, simplifies the process of accessing scalable distributed cloud computing resources and leverages additional software for data and computationally intensive modeling. The Tethys software development kit allows users to create web apps for visualizing, analyzing, and modeling Earth Observation data. Tethys platform provides a collaborative environment for scientists to develop and deploy several Earth Observation web applications across multiple Tethys portals. We work in partnership with leading regional organizations world-wide to help developing …

Characterizing Cdk8/19 Inhibitors Through A Nfκb-Dependent Cell-Based Assay, Jng Li, Donald C. Porter, Eugenia Broude, Igor Roninson, Mengqian Chen

Faculty Publications

Cell-based assays for CDK8/19 inhibition are not easily defined, since there are no known cellular functions unique to these kinases. To solve this problem, we generated derivatives of 293 cells with CRISPR knockout of one or both of CDK8 and CDK19. Double knockout (dKO) of CDK8 and CDK19 together (but not individually) decreased the induction of transcription by NFκB (a CDK8/19-potentiated transcription factor) and abrogated the effect of CDK8/19 inhibitors on such induction. We generated wild type (WT) and dKO cell lines expressing luciferase from an NFκB-dependent promoter. Inhibitors selective for CDK8/19 over other CDKs decreased TNFα-induced luciferase expression in …

The Gut-Microbiome In Gulf War Veterans: A Preliminary Report, Patricia A. Janulewicz, Ratanesh K. Seth, Jeffrey M. Carlson, Joy Ajama, Emily Quinn, Timothy Heeren, Nancy Klimas, Steven M. Lasley, Ronnie Horner, Kimberly Sullivan, Saurabh Chatterjee

Faculty Publications

Gulf War Illness (GWI) is a chronic multi-symptom disorder affecting the central nervous system (CNS), immune and gastrointestinal (GI) systems of Gulf War veterans (GWV). We assessed the relationships between GWI, GI symptoms, gut microbiome and inflammatory markers in GWV from the Boston Gulf War Illness Consortium (GWIC). Three groups of GWIC veterans were recruited in this pilot study; GWV without GWI and no gastrointestinal symptoms (controls), GWV with GWI and no gastrointestinal symptoms (GWI-GI), GWV with GWI who reported gastrointestinal symptoms (GW+GI). Here we report on a subset of the first thirteen stool samples analyzed. Results showed significantly different …

The Importance Of Self-Monitoring For Behavior Change In Youth: Findings From The Switch® School Wellness Feasibility Study, Gabriella M. Mcloughlin, Richard R. Rosenkranz, Joey A. Lee, Maren M. Wolff, Senlin Chen, David A. Dzewaltowski, Spyridoula Vazou, Lorraine Lanningham-Foster, Douglas A. Gentile, Marisa S. Rosen, Gregory J. Welk

Faculty Publications

School Wellness Integration Targeting Child Health (SWITCH®) is a school wellness implementation initiative focused on building capacity for schools to plan and coordinate wellness programming. Grounded in Social Cognitive Theory (SCT), the purpose of this study was to evaluate the utility of the web-based, self-regulation system on physical activity (PA) behavior outcomes. At pre-test and post-test, students in SWITCH® schools (n = 8) completed the online Youth Activity Profile (YAP) to assess PA and sedentary behavior (SB). Students (n = 513) were categorized into high or low self-monitoring groups (using a median split) based on their use of the web-based …

Using The Clinical Frailty Scale To Predict The Length Of Stay In Otolaryngology Unit In Taiwan, Wei-Kang Tung, Hsiang-Chin Hung, Wei-Chen Tung

Faculty Publications

Frailty was a common syndrome in geriatric clinic and general internal medical wards. Some authors had identified the Clinical Frailty Scale (CFS) as a predictor of length of stay in the acute medicine unit. However, the role of the Clinical Frailty Scale in the length of stay in otolaryngology unit had not been well studied. The objective of this study was to find out the correlation of the CFS in elderly patients admitted to otolaryngology unit and their length of stay. A retrospective medical chart review of 203 elderly patients admitted to the otolaryngology ward from January, 2014 to December, …

Life After Pediatric Critical Illness: Risk Factors For Reduced Health-Related Quality Of Life And Functional Decline, Ramona O. Hopkins

Faculty Publications

Survivors of a critical illness are at risk to develop physical, cognitive, and psychological impairments that can persist for months to years after they leave the hospital. Health-related quality of life (HRQL) and functional abilities are important measures of outcomes after a critical illness, as they reflect not only the effects of the critical illness, its treatment, and comorbid illness but also physical disability, cognitive impairment, and psychological disorders (depression, anxiety, and post-traumatic stress disorder) after a critical illness in adults (1). A review of HRQL in 557 adult survivors of acute respiratory distress syndrome found a substantially lower HRQL …

It's Not The Robot's Fault! Russian And American Perspectives On Responsibility For Robot Harms, Bryant Walker Smith, Andrey Neznamov

Faculty Publications

No abstract provided.

Uncertainty In Employee Status Across Federal Law, Ryan Vacca

Faculty Publications

Numerous federal statutes rely on a distinction between employees and independent contractors. Based on a series of Supreme Court decisions from 1968 through 2003, courts and administrative agencies have used a common law multifactor test to draw this distinction. In an effort to enhance predictability and certainty within and across legislation, these cases have rejected a purposive approach in applying the test. But the Supreme Court has never said which, if any, of the factors are the most important in the analysis, nor has anyone determined whether the underlying purpose-enhancing predictability and certainty-has been attained.

This empirical study uses content …

Longitudinal Effects Of Maternal Love Withdrawal And Guilt Induction On Chinese American Preschoolers' Bullying Aggressive Behavior, Jing Yu, Charissa S.L. Cheah, Craig H. Hart, Chongming Yang, Joseph A. Olsen

Faculty Publications

Copyright © 2019 Cambridge University Press. Bullying has been understudied among preschool children, especially those from Chinese American families. Previous research has also neglected the dimensional effects of psychological control on child bullying development. This study examined two psychological control dimensions, love withdrawal and guilt induction, and their effects on children's bullying aggressive behavior using a longitudinal design. Participants were first-generation Chinese American mothers (N = 133; mean age [Mage] = 37.82) and their preschool children (Mage = 4.48). Chinese immigrant mothers reported their psychologically controlling parenting and teachers rated children's bullying aggressive behaviors in the school setting. Confirmatory factor …

Hinge Region In Dna Packaging Terminase Pul15 Of Herpes Simplex Virus: A Potential Allosteric Target For Antiviral Drugs, Lana F. Thaljeh, J. Ainsley Rothschild, Misagh Naderi, Lyndon M. Coghill, Jeremy M. Brown, Michal Brylinski

Faculty Publications

Approximately 80% of adults are infected with a member of the herpesviridae family. Herpesviruses establish life-long latent infections within neurons, which may reactivate into lytic infections due to stress or immune suppression. There are nine human herpesviruses (HHV) posing health concerns from benign conditions to life threatening encephalitis, including cancers associated with viral infections. The current treatment options for most HHV conditions mainly include several nucleoside and nucleotide analogs targeting viral DNA polymerase. Although these drugs help manage infections, their common mechanism of action may lead to the development of drug resistance, which is particularly devastating in immunocompromised patients. Therefore, …

Sympathetic Innervation Of The Interscapular Brown Adipose Tissue In Mouse, Marie François, Hayden Torres, Clara Huesing, Rui Zhang, Carson Saurage, Nathan Lee, Emily Qualls-Creekmore, Sangho Yu, Christopher D. Morrison, David Burk, Hans Rudolf Berthoud, Heike Münzberg

Faculty Publications

© 2019 The Authors. Annals of the New York Academy of Sciences published by Wiley Periodicals, Inc. on behalf of New York Academy of Sciences. The recent discovery of significant brown fat depots in adult humans has revived discussion of exploiting brown fat thermogenesis in the control of energy balance and body weight. The sympathetic nervous system (SNS) has a key role in the activation of brown fat and functional mapping of its components will be crucial for the development of specific neuromodulation techniques. The mouse is an important species used for molecular genetic modulations, but its small size is …