Digital Commons Network^™

Ecological Interfaces For Improving Mobile Robot Teleoperation, Michael A. Goodrich, Curtis W. Nielsen, Robert W. Ricks

Faculty Publications

Navigation is an essential element of many remote robot operations including search and rescue, reconnaissance, and space exploration. Previous reports on using remote mobile robots suggest that navigation is difficult due to poor situation awareness. It has been recommended by experts in human–robot interaction that interfaces between humans and robots provide more spatial information and better situational context in order to improve an operator’s situation awareness. This paper presents an ecological interface paradigm that combines video, map, and robotpose information into a 3-D mixed-reality display. The ecological paradigm is validated in planar worlds by comparing it against the standard interface …

Effect Of An Herbal Extract Number Ten (Nt) On Body Weight In Rats, David A. York, Sonyja Thomas, Frank L. Greenway, Zhijun Liu, Jennifer C. Rood

Faculty Publications

Background: Chinese herbal extract Number Ten (NT) is a dietary herbal formulation prepared from rhubarb, ginger, astragalus, red sage and tumeric. This study tested the effectiveness of NT in reducing body weight gain in rats. Methods: Sixty female Wistar rats were fed a high fat diet and acclimated to gavage feeding. The rats were divided into five treatment groups: (1) Control (n = 15); (2) NT-H (n = 15), 1.5 g/day; (3) NT-L (n = 10), 0.75 g/day; (4) Pr-fed (n = 10), pair fed to NT-H; (5) d-FF (n = 10), d-fenfluramine 2 mg/kg. Ten rats per group were …

Extracellular Stimuli Specifically Regulate Localized Levels Of Individual Neuronal Mrnas, Dianna E. Willis, Erna A. Van Niekerk, Yukio Sasaki, Mariano Mesngon, Tanuja T. Merianda, Gervan G. Williams, Marvin Kendall, Deanna S. Smith, Gary J. Bassell, Jeffery L. Twiss

Faculty Publications

Subcellular regulation of protein synthesis requires the correct localization of messenger RNAs (mRNAs) within the cell. In this study, we investigate whether the axonal localization of neuronal mRNAs is regulated by extracellular stimuli. By profiling axonal levels of 50 mRNAs detected in regenerating adult sensory axons, we show that neurotrophins can increase and decrease levels of axonal mRNAs. Neurotrophins (nerve growth factor, brainderived neurotrophic factor, and neurotrophin-3) regulate axonal mRNA levels and use distinct downstream signals to localize individual mRNAs. However, myelin-associated glycoprotein and semaphorin 3A regulate axonal levels of different mRNAs and elicit the opposite effect on axonal mRNA …

Sympathy And The Non-Human: Max Scheler’S Phenomenology Of Interrelation, David Dillard-Wright

Faculty Publications

German phenomenologist and sociologist Max Scheler accorded sympathy a central role in his philosophy, arguing that sympathy enables not only ethical behaviour, but also knowledge of animate and inanimate others. Influenced by Catholicism and especially St Francis, Scheler envisioned a broad, cosmic sympathy forming the hidden basis for all human values, with the “higher” religious, artistic, philosophic and other cultural values enabled by a more basic regard for non-human nature and insights gained from the human situation within the non-human world. Sympathy for the non-human is thus both integral and fundamental to the cultivation of other values in the development …

Spiroplasma Spp. From Transmissible Spongiform Encephalopathy Brains Or Ticks Induce Spongiform Encephalopathy In Ruminants, Frank O. Bastian, Dearl E. Sanders, Will A. Forbes, Sue D. Hagius, Joel V. Walker, William G. Henk, Fred M. Enright, Philip H. Elzer

Faculty Publications

Spiroplasma, small motile wall-less bacteria, are linked by molecular and serological studies to the transmissible spongiform encephalopathies (TSEs), which include scrapie in sheep, chronic wasting disease (CWD) in deer and Creutzfeldt-Jakob disease in humans. In this study, two experiments were undertaken to determine the role of spiroplasma in the pathogenesis of TSE. In experiment 1, Spiroplasma mirum, a rabbit tick isolate that had previously been shown to experimentally induce spongiform encephalopathy in rodents, was inoculated intracranially (IC) into ruminants. S. mirum-inoculated deer manifested clinical signs of TSE after 1.5 to 5.5 months incubation. The deer, as well as sheep and …

Spiroplasma Spp. From Transmissible Spongiform Encephalopathy Brains Or Ticks Induce Spongiform Encephalopathy In Ruminants, Frank O. Bastian, Dearl E. Sanders, Will A. Forbes, Sue D. Hagius, Joel V. Walker, William G. Henk, Fred M. Enright, Philip H. Elzer

Faculty Publications

Spiroplasma, small motile wall-less bacteria, are linked by molecular and serological studies to the transmissible spongiform encephalopathies (TSEs), which include scrapie in sheep, chronic wasting disease (CWD) in deer and Creutzfeldt-Jakob disease in humans. In this study, two experiments were undertaken to determine the role of spiroplasma in the pathogenesis of TSE. In experiment 1, Spiroplasma mirum, a rabbit tick isolate that had previously been shown to experimentally induce spongiform encephalopathy in rodents, was inoculated intracranially (IC) into ruminants. S. mirum-inoculated deer manifested clinical signs of TSE after 1.5 to 5.5 months incubation. The deer, as well as sheep and …

Comparative Microbiota Of Rickettsia Felis-Uninfected And -Infected Colonized Cat Fleas, Ctenocephalides Felis, Walairat Pornwiroon, Michael T. Kearney, Claudia Husseneder, Lane D. Foil, Kevin R. Macaluso

Faculty Publications

Fleas serve as arthropod vectors for several emerging and re-emerging infectious disease causing agents including, Rickettsia felis. Although the prevalence of R. felis infection in colonies of fleas has been examined, the influence of the R. felis infection on flea microbiota has not been investigated. We identified three colonies of cat fleas, Ctenocephalides felis, with varying prevalence of R. felis infection (Louisiana State University (LSU), 93.8; Professional Laboratory and Research Services Inc. (PLRS), 16.4; Elward II (EL), 0) and subsequently utilized polymerase chain reaction amplification, restriction fragment length polymorphism analysis and sequencing of the 1.4-kb portions of 16S rRNA genes …

Footprints On Water: The Genetic Wake Of Dispersal Among Reefs, M. E. Hellberg

Faculty Publications

Analysis of genetic data can reveal past and ongoing demographic connections between reef populations. The history, extent, and geography of isolation and exchange help to determine which populations are evolutionarily distinct and how to manage threatened reefs. Here the genetic approaches undertaken to understand connectivity among reefs are reviewed, ranging from early allozyme studies on genetic subdivision, through the use of sequence data to infer population histories, to emerging analyses that pull the influences of the past connections away from the effects of ongoing dispersal. Critically, some of these new approaches can infer migration and isolation over recent generations, thus …

Description, Life History, And Parasitism Of A New Species Of Delphacid Planthopper (Hemiptera: Fulgoroidea), James T. Cronin, Stephen W. Wilson

Faculty Publications

Delphacodes scolochloa Cronin & Wilson (Hemiptera: Fulgoroidea) is a newly discovered delphacid planthopper that feeds and oviposits exclusively on the stems of sprangletop, Scolochloa festucacea (Willd.) Link (Poaceae: Pooidae), in the prairie pothole region of northeastern North Dakota. D. scolochloa is bivoltine. It is also wing dimorphic, but populations are composed predominantly of flightless brachypters. Macropters incur a substantial cost in terms of reduced longevity and fecundity relative to brachypters. The parasitoid complex attacking D. scolochloa consists of two egg parasitoids, Anagrus nigriventris Girault and Anagrus columbi Perkins (Hymenoptera: Mymaridae), and one undescribed dryinid. The proportion of eggs parasitized averages …

Historic And Contemporary Levels Of Genetic Variation In Two New Zealand Passerines With Different Histories Of Decline, S. S. Taylor, I. G. Jamieson, G. P. Wallis

Faculty Publications

We compared historic and contemporary genetic variation in two threatened New Zealand birds (saddlebacks and robins) with disparate bottleneck histories. Saddlebacks showed massive loss of genetic variation when extirpated from the mainland, but no significant loss of variation following a severe bottleneck in the 1960s when the last population was reduced from ∼1000 to 36 birds. Low genetic variation was probably characteristic of this isolated island population: considerably more genetic variation would exist in saddlebacks today if a mainland population had survived. In contrast to saddlebacks, contemporary robin populations showed only a small decrease in genetic variation compared with historical …

Differential Resource Allocation In Deer Mice Exposed To Sin Nombre Virus, Eric Wilson, Erin M. Lehmer, Christine A. Clay, Stephen St. Jeor, Denise M. Dearing

Faculty Publications

The resource allocation hypothesis predicts that reproductive activity suppresses immunocompetence; however, this has never been tested in an endemic disease system with free-ranging mammals. We tested the resource allocation hypothesis in wild deer mice (Peromyscus maniculatus) with natural exposure to Sin Nombre Virus (SNV). Immunocompetence was estimated from the extent of swelling elicited after deer mice were injected with phytohemagglutinin (PHA); swelling is positively correlated with immunocompetence. After livetrapping deer mice, we determined their reproductive state and SNV infection status. Males were more likely to be seropositive for SNV than females (37% vs. 25%) and exhibited 10% less swelling after …

Comparative Mitochondrial Genomics Of Snakes: Extraordinary Substitution Rate Dynamics And Functionality Of The Duplicate Control Region, Zhi J. Jiang, Todd A. Castoe, Christopher C. Austin, Frank T. Burbrink, Matthew D. Herron, Jimmy A. Mcguire, Christopher L. Parkinson, David D. Pollock

Faculty Publications

Background. The mitochondrial genomes of snakes are characterized by an overall evolutionary rate that appears to be one of the most accelerated among vertebrates. They also possess other unusual features, including short tRNAs and other genes, and a duplicated control region that has been stably maintained since it originated more than 70 million years ago. Here, we provide a detailed analysis of evolutionary dynamics in snake mitochondrial genomes to better understand the basis of these extreme characteristics, and to explore the relationship between mitochondrial genome molecular evolution, genome architecture, and molecular function. We sequenced complete mitochondrial genomes from Slowinski's corn …

Alvin Explores The Deep Northern Gulf Of Mexico Slope, Harry Roberts, Robert Carney, Mathew Kupchik, Charles Fisher, Kim Nelson, Erin Becker, Liz Goehring, Stephanie Lessard-Pilon, Guy Telesnicki, Bernie Bernard, James Brooks, Monika Bright, Erik Cordes, Stephane Hourdez, Jesse Hunt, William Shedd, Gregory Boland, Samantha Joye, Vladimir Samarkin, Meaghan Bernier, Marshall Bowles, Ian Macdonald, Helge Niemann, Cindy Petersen, Jeremy Potter

Faculty Publications

No abstract provided.

The Benefits Of Bathing Buds: Water Calyces Protect Flowers From A Microlepidopteran Herbivore, Jane E. Carlson, Kyle E. Harms

Faculty Publications

Protective floral structures may evolve in response to the negative effects of floral herbivores. For example, water calyces - liquid-filled, cup-like structures resulting from the fusion of sepals - may reduce floral herbivory by submerging buds during their development. Our observations of a water-calyx plant, Chrysothemis friedrichsthaliana (Gesneriaceae), revealed that buds were frequently attacked by ovipositing moths (Alucitidae), whose larvae consumed anthers and stigmas before corollas opened. Almost 25% of per-plant flower production was destroyed by alucitid larvae over two seasons, far exceeding the losses to all other floral herbivores combined. Experimental manipulation of water levels in calyces showed that …

The Katanin Microtubule Severing Protein In Plants, David H. Burk, Ruiqin Zhong, Zheng Hua Ye

Faculty Publications

Katanin is a heterodimeric microtubule (MT) severing protein that uses energy from ATP hydrolysis to generate internal breaks along MTs. Katanin p60, one of the two subunits, possesses ATPase and MT-binding/ severing activities, and the p 80 subunit is responsible for targeting of katanin to certain subcellular locations. In animals, katanin plays an important role in the release of MTs from their nucleation sites in the centrosome. It is also involved in severing MTs into smaller fragments which can serve as templates for further polymerization to increase MT number during meiotic and mitotic spindle assembly. Katanin homologs are present in …

Interventional Closure Of Postpneumonectomy Bronchial Pleural Fistula With A Self-Expandable Double Umbrella-Shaped Occluder Knitted With Nitinol Shape Memory Alloy, Jianhua Zhang, Shengshou Hu, Bingren Gao, Debin Liu, Feixue Song, Bin Li, Yongzhu Yang, Shenjun Zhu, Zhiping Wang

Faculty Publications

No abstract provided.

Seed Preferences Of Wintering Henslow's Sparrows, Jennifer K. Dimiceli, Philip C. Stouffer, Erik I. Johnson, Claudia Leonardi, Edgar B. Moser

Faculty Publications

The Henslow's Sparrow (Ammodramus henslowii), a species of conservation concern, winters primarily in longleaf pine (Pinus palustris) forest ecosystems in the southeastern U.S. These pine savannas have been reduced to 5% of their former range, with remaining patches requiring active management with fire to maintain characteristic structure and plant diversity. Wintering Henslow's Sparrow abundance tracks growing-season fires; bird abundance peaks in the winter following burning, then declines in subsequent winters. Fire also determines dominant plant species, suggesting that Henslow's Sparrows may respond to abundance of preferred seeds. To determine diet preferences of Henslow's Sparrows, we tested seeds from eight species …

Assessment Of Genetic Diversity In The Usda And Cip-Fao International Nursery Collections Of Quinoa (Chenopodium Quinoa Willd.) Using Microsatellite Markers, S. A. Christensen, D. B. Pratt, C. Pratt, P. T. Nelson, M. R. Stevens, Eric N. Jellen, Craig E. Coleman, Daniel J. Fairbanks, A. Bonifacio, Michael J. Maughan

Faculty Publications

Quinoa (Chenopodium quinoa Willd.) is a staple food crop for millions of impoverished rural inhabitants of Andean South America where it has been cultivated for millennia. Interest in quinoa, due largely to its superior nutritional characteristics, is fuelling a growing export market and has led to an increased focus on genetic research and the development of quinoa breeding programmes throughout South America. The success of these breeding programmes will rely heavily on the development of core germplasm collections and germplasm conservation. We report the development of a set of fluorescence-tagged microsatellite molecular markers that can be used to characterize genetic …

Development Of A Simplified And Standardized Protocol With Potential For High-Throughput For Sperm Cryopreservation In Zebrafish Danio Rerio, Huiping Yang, Carrie Carmichael, Zoltan M. Varga, Terrence R. Tiersch

Faculty Publications

Sperm cryopreservation offers potential for long-term storage of genetic resources. However, the current protocols for zebrafish Danio rerio are cumbersome and poorly reproducible. Our objective was to facilitate adoption of cryopreservation by streamlining methods from sperm collection through thawing and use. First, sperm activation was evaluated, and motility was completely inhibited when osmolality of the extender was ≥295-300 mOsmol/kg. To evaluate cryoprotectant toxicity, sperm were incubated with dimethyl sulfoxide (DMSO), N,N-dimethyl acetamide (DMA), methanol, or glycerol at 5, 10, and 15% concentrations. Based on motility, DMSO, DMA, and methanol (≤10%) were less toxic; therefore, sperm were cryopreserved using these cryoprotectants …

Extended Abstract Rotopod: A Novel Approach To Efficient Legged Locomotion, Damian M. Lyons

Faculty Publications

A number of attempts have been made to integrate the efficiency of wheeled locomotion with the terrain versatility of legged locomotion, e.g., Univ.Michigan’s Rhex platform and Case Western’s Whegs. Those platforms cast legs as rotating spokes placed traditionally at the corners of a rectangular platform. In this paper, we present an alternate approach, with three legs radiating down from a central hub. The energy to move the platform is generated by a rotating reaction mass mounted at the hub and, at rest, rotating parallel to the ground plane.

Our approach is to construct a platform whose natural, uncontrolled motion is …

"Walk In The Spirit": Preaching For Spiritual Growth (Gal. 5:13-6:2), Alan G. Padgett

Faculty Publications

Congregations are hungry for a biblical and trinitarian spirituality. With a sustained biblical leadership, local churches can be places where a genuine evangelical and trinitarian spirituality is reclaimed for our time.

Unidirectional Na+ And Ca2+ Fluxes In Two Euryhaline Teleost Fishes, Fundulus Heteroclitus And Oncorhynchus Mykiss, Acutely Submitted To A Progressive Salinity Increase, Viviane Prodocimo, Fernando Galvez, Carolina A. Freire, Chris M. Wood

Faculty Publications

Na+ and Ca2+ regulation were compared in two euryhaline species, killifish (normally estuarine-resident) and rainbow trout (normally freshwater-resident) during an incremental salinity increase. Whole-body unidirectional fluxes of Na+ and Ca2+, whole body Na+ and Ca2+, and plasma concentrations (trout only), were measured over 1-h periods throughout a total 6-h protocol of increasing salinity meant to simulate a natural tidal flow. Killifish exhibited significant increases in both Na+ influx and efflux rates, with efflux slightly lagging behind efflux up to 60% SW, but net Na+ balance was restored by the time killifish reached 100% SW. Whole body Na+ did not change, …

Energizing An Invertebrate Embryo: Bafilomycin-Dependent Respiration And The Metabolic Cost Of Proton Pumping By The V-Atpase, Joseph A. Covi, Steven C. Hand

Faculty Publications

We examine herein the contribution of V-ATPase activity to the energy budget of aerobically developing embryos of Artemia franciscana and discuss the results in the context of quiescence under anoxia. 31P-NMR analysis indicates that intracellular pH and NTP levels are unaffected by acute incubation of dechorionated embryos with the V-ATPase inhibitor, bafilomycin A1. Bafilomycin A1 also has no significant effect on oxygen consumption by isolated mitochondria. Taken together, these data indicate that bafilomycin does not affect energy-producing pathways in the developing embryo. However, the V-ATPase inhibitor exhibits a concentration-dependent inhibition of oxygen consumption in aerobic embryos. A conservative analysis of …

Sines, Evolution And Genome Structure In The Opossum, Wanjun Gu, David A. Ray, Jerilyn A. Walker, Erin W. Barnes, Andrew J. Gentles, Paul B. Samollow, Jerzy Jurka, Mark A. Batzer, David D. Pollock

Faculty Publications

Short INterspersed Elements (SINEs) are non-autonomous retrotransposons, usually between 100 and 500 base pairs (bp) in length, which are ubiquitous components of eukaryotic genomes. Their activity, distribution, and evolution can be highly informative on genomic structure and evolutionary processes. To determine recent activity, we amplified more than one hundred SINE1 loci in a panel of 43 M. domestica individuals derived from five diverse geographic locations. The SINE1 family has expanded recently enough that many loci were polymorphic, and the SINE1 insertion-based genetic distances among populations reflected geographic distance. Genome-wide comparisons of SINE1 densities and GC content revealed that high SINE1 …

Genomic Organization And Imprinting Of The Peg3 Domain In Bovine, Joomyeong Kim, Anne Bergmann, Jung Ha Choo, Lisa Stubbs

Faculty Publications

Using multiple mammalian genomic sequences, we have analyzed the evolution and imprinting of several genes located in the Peg3 domain, including Mim1 (approved name, Mimt1), Usp29, Zim3, and Zfp264. A series of comparative analyses shows that the overall genomic structure of this 500-kb imprinted domain has been well maintained throughout mammalian evolution but that several lineage-specific changes have also occurred in each species. In the bovine domain, Usp29 has lost its protein-coding capability, Zim3 has been duplicated, and the expression of Zfp264 has become biallelic in brain and testis, which differs from paternal expression of mouse Zfp264 in brain. In …

Population Spread Of The Introduced Red Imported Fire Ant Parasitoid, Pseudacteon Tricuspis Borgmeier (Diptera: Phoridae), In Louisiana, D. C. Henne, S. J. Johnson, J. T. Cronin

Faculty Publications

Predicting the spread of introduced species, such as natural enemies used in classical biological control programs, requires quantitative data on the rates of spread. Here, the pattern of spread of Pseudacteon tricuspis Borgmeier (Diptera: Phoridae), a parasitoid of the red imported fire ant (Solenopsis invicta Buren; Hymenoptera: Formicidae), was monitored at two widely separated release sites in Louisiana, USA. At both sites, P. tricuspis range expansion (measured as the mean radius of the range from four cardinal directions) was accelerating during the first four years post-release. This pattern contrasts with a linear pattern expected with simple diffusion. This suggests that …

Mentors And Students Seeking Knowledge, Meredith Jones-Gray

Faculty Publications

No abstract provided.

Information Consensus And Its Application In Multi-Vehicle Cooperative Control, Ella Atkins, Randal Beard, Wei Ren

Faculty Publications

In the last two decades, advances in networking and distributed computing have facilitated a paradigm shift from large, monolithic mainframe computers to networks of less expensive, less powerful workstations. One motivation for multi-vehicle systems is to achieve the same gains for mechanically controlled systems as has been gained in distributed computation. Rather than having a single monolithic (and therefore expensive and complicated) machine do everything, the hope is that many inexpensive, simple machines, can achieve the same, or enhanced functionality, through coordination. In essence, the objective is to replace expensive complicated hardware with software and multiple copies of simple hardware. …

A Multi-Scale Examination Of Stopover Habitat Use By Birds, Jeffrey J. Buler, Frank R. Moore, Stefan Woltmann

Faculty Publications

Most of our understanding of habitat use by migrating land birds comes from studies conducted at single, small spatial scales, which may overemphasize the importance of intrinsic habitat factors, such as food availability, in shaping migrant distributions. We believe that a multi-scale approach is essential to assess the influence of factors that control en route habitat use. We determined the relative importance of eight variables, each operating at a habitat-patch, landscape, or regional spatial scale, in explaining the differential use of hardwood forests by Nearctic-Neotropical land birds during migration. We estimated bird densities through transect surveys at sites near the …

Genetic Evolution Of Hierarchical Behavior Structures, Brian G. Woolley, Gilbert L. Peterson

Faculty Publications

The development of coherent and dynamic behaviors for mobile robots is an exceedingly complex endeavor ruled by task objectives, environmental dynamics and the interactions within the behavior structure. This paper discusses the use of genetic programming techniques and the unified behavior framework to develop effective control hierarchies using interchangeable behaviors and arbitration components. Given the number of possible variations provided by the framework, evolutionary programming is used to evolve the overall behavior design. Competitive evolution of the behavior population incrementally develops feasible solutions for the domain through competitive ranking. By developing and implementing many simple behaviors independently and then evolving …