Digital Commons Network^™

A Covalent Linker Allows For Membrane Targeting Of An Oxylipin Biosynthetic Complex, Nathaniel C. Gilbert, Marc Niebuhr, Hiro Tsuruta, Tee Bordelon, Oswin Ridderbusch, Adam Dassey, Alan R. Brash, Sue G. Bartlett, Marcia E. Newcomer

Faculty Publications

A naturally occurring bifunctional protein from Plexaura homomalla links sequential catalytic activities in an oxylipin biosynthetic pathway. The C-terminal lipoxygenase (LOX) portion of the molecule catalyzes the transformation of arachidonic acid (AA) to the corresponding 8R-hydroperoxide, and the N-terminal allene oxide synthase (AOS) domain promotes the conversion of the hydroperoxide intermediate to the product allene oxide (AO). Small-angle X-ray scattering data indicate that in the absence of a covalent linkage the two catalytic domains that transform AA to AO associate to form a complex that recapitulates the structure of the bifunctional protein. The SAXS data also support a model for …

Powerpoint In Preaching? Yes...But!, Andrew Root

Faculty Publications

No abstract provided.

Effacement And Metaphor: Searching For The Body In Educational Discourse, Keith Nainby, Deanna Fassett

Faculty Publications

This essay concerns the body’s positioning in discussions of teaching, specifically focusing on the authors’ efforts to trace discursive invocations of teachers’ bodies by students reflecting on the teaching vocation. The authors explore, through a series of intertwined autoethnographic narratives, the research process that led them through focus group data collection and analysis, to reflections on students’ metaphorical use of the “teacher’s body” in these focus groups, to (in light of feedback from anonymous reviewers) the role of the authors’ own teachers’ bodies in constituting this research and its implications.

Black Bears On The Way Back, Christopher E. Comer

Faculty Publications

No abstract provided.

Red-Cockaded Woodpeckers And Hurricanes, Zoe Hoyle

Faculty Publications

No abstract provided.

Viewing Virtual Property Ownership Through The Lens Of Innovation, Ryan Vacca

Faculty Publications

Over the past several years, many scholars have wrestled with the idea of how property rights for items created in virtual worlds should be conceptualized. Some have discussed utilitarian models, others have examined a Lockean natural rights theory, and still others view virtual property merely as another form of intellectual property. Regardless of how virtual property is conceptualized and which theory it best fits, most, if not all, commentators agree that the law ought to recognize virtual property as property and vest someone with those rights.

This Article moves beyond the initial debate about how property rights in virtual property …

Toll-Like Receptor 2 Recognition Of The Microsporidia Encephalitozoon Spp. Induces Nuclear Translocation Of Nf-Κb And Subsequent Inflammatory Responses, Jeffrey Fischer, Colby Suire, Hollie Hale-Donze

Faculty Publications

Microsporidia are obligate intracellular parasites that are ubiquitous in nature and have been recognized as causing an important emerging disease among immunocompromised individuals. Limited knowledge exists about the immune response against these organisms, and virtually nothing is known about the receptors involved in host recognition. Toll-like receptors (TLR) are pattern recognition receptors that bind to specific molecules found on pathogens and signal a variety of inflammatory responses. In this study, we show that both Encephalitozoon cuniculi and Encephalitozoon intestinalis are preferentially recognized by TLR2 and not by TLR4 in primary human macrophages. This is the first demonstration of host receptor …

Jamaican Nightshade (Solanum Jamaicense): A Threat To Florida's Hammocks, Rodrigo Diaz, William A. Overholt, Kenneth Langeland

Faculty Publications

Jamaican nightshade is a prickly, perennial, invasive shrub in central and southern peninsular Florida. It was first seen in Florida in 1930 near St. Cloud, and has since been reported at several other locations in the state. Jamaican nightshade is primarily found in wooded habitats, where it can quickly become dominant in the understory, but it also occasionally grows in isolated patches in the open. Although the distribution of Jamaican nightshade does not appear to be rapidly expanding in Florida, land mangers should be made aware of the potential of this weed to establish at new sites, and initiate control …

Bias In Population Growth Rate Estimation: Sparse Data, Partial Life Cycle Analysis And Jensen's Inequality, Jason S. Grear, Bret D. Elderd

Faculty Publications

Demographic matrix models have become an integral part of population viability analysis for threatened and endangered species, but their use is often limited by data availability. A common solution to this problem is to assume constant annual rates within a multi-year stage. Partial life cycle analysis (PLC), which incorporates only juvenile and adult stages, is a noteworthy example of this approach because it has been described in the literature as a reliable approximation of age-structured populations. However, we predict from Jensen's Inequality that the required lumping of age classes leads to over- or underestimation of population fitness when survival rates …

Combustion Byproducts And Their Health Effects: Summary Of The 10 Th International Congress, Barry Dellinger, Antonio D'Alessio, Andrea D'Anna, Anna Ciajolo, Brian Gullett, Heather Henry, Mel Keener, Jo Ann Lighty, Slawomir Lomnicki, Donald Lucas, Günter Oberdörster, Demetrio Pitea, William Suk, Adel Sarofim, Kirk R. Smith, Tobias Stoeger, Paige Tolbert, Ron Wyzga, Ralf Zimmermann

Faculty Publications

The 10th International Congress on Combustion Byproducts and their Health Effects was held in Ischia, Italy, from June 17-20, 2007. It is sponsored by the US NIEHS, NSF, Coalition for Responsible Waste Incineration (CRWI), and Electric Power Research Institute (EPRI). The congress focused on: the origin, characterization, and health impacts of combustion-generated fine and ultrafine particles; emissions of mercury and dioxins, and the development/application of novel analytical/diagnostic tools. The consensus of the discussion was that particle-associated organics, metals, and persistent free radicals (PFRs) produced by combustion sources are the likely source of the observed health impacts of airborne PM rather …

How To Know If A Bible Principle Applies Today, Roy Gane

Faculty Publications

No abstract provided.

From Pulp Hero To Superhero: Culture, Race, And Identity In American Popular Culture, 1900-1940, Julian C. Chambliss, William L. Svitavsky

Faculty Publications

Adventure characters in the pulp magazines and comic books of the early twentieth century reflected development in the ongoing American fascination with heroic figures. As established figures such as the cowboy became disconnected from everyday experiences of Americans, new popular fantasies emerged, providing readers with essentialist action heroes whose adventures stylized the struggle of the American everyman with a modern, industrialized, heterogeneous world. Popular characters such as Tarzan, Conan, the Shadow, and Doc Savage perpetuated the individualistic archetype Americans associated with the frontier cowboy and the struggles of manifest destiny while offering the fantastic adventure, exoticism, and escapism that modernity …

Modeling The Daily Activities Of Breeding Colonial Seabirds: Dynamic Occupancy Patterns In Multiple Habitat Patches, Andrea L. Moore, Smruti P. Damania, Shandelle M. Henson, James L. Hayward

Faculty Publications

We constructed differential equation models for the diurnal abundance and distribution of breeding glaucous-winged gulls (Larus glaucescens) as they moved among nesting and non-nesting habitat patches. We used time scale techniques to reduce the differential equations to algebraic equations and connected the models to field data. The models explained the data as a function of abiotic environmental variables with R2=0.57. A primary goal of this study is to demonstrate the utility of a methodology that can be used by ecologists and wildlife managers to understand and predict daily activity patterns in breeding seabirds.

A Darwinist View Of The Living Constitution, Scott Dodson

Faculty Publications

The metaphor of a “living” Constitution imports terms from biology into law and, in the process, relies on biology for its meaning. A proper understanding of biology is therefore central to understanding the idea of “living” constitutionalism. Yet despite its rampant use by both opponents and proponents of living constitutionalism, and despite the current fervent debate over whether biology can be useful to the law, no one has evaluated the metaphor from a biological perspective. This Essay begins that inquiry in an interdisciplinary study of law, science, and philology. The Essay first evaluates the metaphor as it is currently used …

Archives And Justice: A South African Perspective By Verne Harris, With A Foreword By Terry Cook. Chicago: Society Of American Archivists, 2007. Isbn 1-931666-18-0., Jennifer A. Marshall

Faculty Publications

No abstract provided.

Newly Discovered Sister Lineage Sheds Light On Early Ant Evolution, Christian Rabeling, Jeremy M. Brown, Manfred Verhaagh

Faculty Publications

Ants are the world's most conspicuous and important eusocial insects and their diversity, abundance, and extreme behavioral specializations make them a model system for several disciplines within the biological sciences. Here, we report the discovery of a new ant that appears to represent the sister lineage to all extant ants (Hymenoptera: Formicidae). The phylogenetic position of this cryptic predator from the soils of the Amazon rainforest was inferred from several nuclear genes, sequenced from a single leg. Martialis heureka (gen. et sp. nov.) also constitutes the sole representative of a new, morphologically distinct subfamily of ants, the Martialinae (subfam. nov.). …

Sequestration And Ultrasound-Induced Release Of Doxorubicin From Stabilized Pluronic P105 Micelles, John D. Pruitt, William G. Pitt

Faculty Publications

Controlled drug delivery from micelles requires that the micelles remain stable when diluted below their critical micelle concentration, such as upon injection into blood. A cross-linked, interpenetrating network of N,N-diethylacrylamide (NNDEA) was polymerized in the core of Pluronic P105 micelles to stabilize temporarily the micelles at concentrations below the critical micellar concentration of free P105. The stabilized Pluronic micelles (called Plurogels) were able to sequester the drug doxorubicin (Dox) and protect HL-60 cells from the drug at concentrations where non-stabilized Pluronic provided no protection. The protection lasted ~ 12 hr, which is similar to the half-life of the particles. Application …

Human Embryonic Stem Cell Research, Justice, And The Problem Of Unequal Biological Access, Mark Moller

Faculty Publications

No abstract provided.

Lis1 And Ndel1 Influence The Timing Of Nuclear Envelope Breakdown In Neural Stem Cells, Sachin Hebbar, Mariano T. Mesngon, Aimee M. Guillotte, Bhavim Desai, Ramses Ayala, Deanna S. Smith

Faculty Publications

Lis1 and Ndel1 are essential for animal development. They interact directly with one another and with cytoplasmic dynein. The developing brain is especially sensitive to reduced Lis1 or Ndel1 levels, as both proteins influence spindle orientation, neural cell fate decisions, and neuronal migration. We report here that Lis1 and Ndel1 reduction in a mitotic cell line impairs prophase nuclear envelope (NE) invagination (PNEI). This dyneindependent process facilitates NE breakdown (NEBD) and occurs before the establishment of the bipolar spindle. Ndel1 phosphorylation is important for this function, regulating binding to both Lis1 and dynein. Prophase cells in the ventricular zone (VZ) …

Rotational Grazing Adoption In Cattle Production Under A Cost-Share Agreement: Does Uncertainty Have A Role In Conservation Technology Adoption?, Seon Ae Kim, Jeffrey M. Gillespie, Krishna P. Paudel

Faculty Publications

Rotational grazing has been promoted as a best management practice with environmental benefits and associated higher revenue. Its adoption rate has been relatively low. This study investigates the role of uncertainty in the adoption of rotational grazing with a cost-share by cattle producers. Mail survey results indicate that 63-71 per cent of cattle producers are uncertain about adoption with a government cost-share. The study suggests that the possibility of uncertainty should be considered in cases where willingness-to-pay is elicited in the context of adoption of technology. © Journal compilation © 2008 Australian Agricultural and Resource Economics Society Inc. and Blackwell …

Many Species In One: Dna Barcoding Overestimates The Number Of Species When Nuclear Mitochondrial Pseudogenes Are Coamplified, Michael F. Whiting, Hojun Song, Keith A. Crandall, Jennifer E. Buhay

Faculty Publications

Nuclear mitochondrial pseudogenes (numts) are nonfunctional copies of mtDNA in the nucleus that have been found in major clades of eukaryotic organisms.

Leading In The Midst Of Change: A Theologically Grounded, Theoretically Informed Hermeneutic Of Change, Terri L. Elton

Faculty Publications

This essay proposes a hermeneutic of change, grounded in theology and theory, which can inform church leaders’ strategic actions in the midst of change. Drawing from the work of practical theology, it looks at four vantage points proposed by Don Browning: descriptive, historical, systematic, and strategic. The descriptive view offers two insights: God is active and present in the midst of change and God’s people are simultaneously saints and sinners. The historical perspective points out that God has always been in the midst of change, but God’s love and promises for the world have not changed. Systematic theology fuses the …

Control Of Neuronal Persistent Activity By Voltage-Dependent Dendritic Properties., Idoux E, Eugène D, Chambaz A, Magnani C, White Ja, Moore Le

Faculty Publications

No abstract provided.

Diffusion Models For Animals In Complex Landscapes: Incorporating Heterogeneity Among Substrates, Individuals And Edge Behaviours, John D. Reeve, James T. Cronin, Kyle J. Haynes

Faculty Publications

1. Animals move commonly through a variety of landscape elements and edges in search of food, mates and other resources. We developed a diffusion model for the movement of an insect herbivore, the planthopper Prokelisia crocea, that inhabits a landscape composed of patches of its host plant, prairie cordgrass Spartina pectinata, embedded in a matrix of mudflat or smooth brome Bromus inermis. 2. We used mark-release-resight experiments to quantify planthopper movements within cordgrass-brome and cordgrass-mudflat arenas. A diffusion model was then fitted that included varying diffusion rates for cordgrass and matrix, edge behaviour in the form of a biased random …

Inchoate Cd8+ T Cell Responses In Neonatal Mice Permit Influenza-Induced Persistent Pulmonary Dysfunction, Dahui You, Michael Ripple, Shrilatha Balakrishna, Dana Troxclair, Dane Sandquist, Liren Ding, Terry A. Ahlert, Stephania A. Cormier

Faculty Publications

Influenza infection remains a significant cause of pulmonary morbidity and mortality worldwide, with the highest hospitalization and mortality rates occurring in infants and elder adults. The mechanisms inducing this considerable morbidity and mortality are largely unknown. To address this question, we established a neonatal mouse model of influenza infection to test the hypothesis that the immaturity of the neonatal immune system is responsible for the severe pulmonary disease observed in infants. Seven-day-old mice were infected with influenza A virus (H1N1) and allowed to mature. As adults, these mice showed enhanced airway hyperreactivity, chronic pulmonary inflammation, and diffuse emphysematous-type lesions in …

Wtse, An Avre-Family Type Iii Effector Protein Of Pantoea Stewartii Subsp. Stewartii, Causes Cell Death In Non-Host Plants, Jong Hyun Ham, Doris Majerczak, Sophie Ewert, Mysore Venkatarau Sreerekha, David Mackey, David Coplin

Faculty Publications

Pantoea stewartii subsp. stewartii (Pnss) causes Stewart's bacterial wilt of sweet corn and leaf blight of maize. The pathogenicity of Pnss depends on synthesis of extracellular polysaccharide and an Hrp type III secretion system. WtsE, a type III secreted effector protein, is essential for the virulence of Pnss on corn. It belongs to the AvrE family of effectors, which includes DspA/E from Erwinia amylovora and AvrE1 from Pseudomonas syringae. Previously, WtsE was shown to cause disease-associated cell death in its host plant, sweet corn. Here, we examine the biological activity of WtsE in several non-host plants. WtsE induced cell death …

Diagnoses For Leiognathus Lacepède 1802, Equula Cuvier 1815, Equulites Fowler 1904, Eubleekeria Fowler 1904, And A New Ponyfish Genus (Teleostei: Leiognathidae), Prosanta Chakrabarty, John S. Sparks

Faculty Publications

Recent phylogenetic work on ponyfishes has delimited and revealed the interrelationships of major leiognathid clades. To begin to recognize a monophyletic taxonomy for Leiognathidae we diagnose, delimit, and describe a number of ponyfish genera to reflect our current knowledge of their phylogenetic history. Equula Cuvier, 1815, is resurrected from synonymy with Leiognathus Lacepède, 1802, and comprises E. fasciata and E longispinis. Members of Equula, the sister group to all other members of Leiognathidae, are large-bodied ponyfishes characterized by conspicuous round yellow blotches on the flank. The genus Leiognathus Lacepède, 1802, the sister group to the remaining members of Leiognathidae less …

Estimating Species Trees Using Multiple-Allele Dna Sequence Data, Liang Liu, Dennis K. Pearl, Robb T. Brumfield, Scott V. Edwards

Faculty Publications

Several techniques, such as concatenation and consensus methods, are available for combining data from multiple loci to produce a single statement of phylogenetic relationships. However, when multiple alleles are sampled from individual species, it becomes more challenging to estimate relationships at the level of species, either because concatenation becomes inappropriate due to conflicts among individual gene trees, or because the species from which multiple alleles have been sampled may not form monophyletic groups in the estimated tree. We propose a Bayesian hierarchical model to reconstruct species trees from multiple-allele, multilocus sequence data, building on a recently proposed method for estimating …

Viability And Fertilizing Capacity Of Cryopreserved Sperm From Three North American Acipenseriform Species: A Retrospective Study, Á Horváth, W. R. Wayman, J. C. Dean, B. Urbányi, T. R. Tiersch, S. D. Mims, D. Johnson, J. A. Jenkins

Faculty Publications

Populations of sturgeon across the globe are threatened due to unregulated harvest and habitat loss, and the status varies among species across North America. Ready access to viable and functional sperm would contribute to recovery programmes for these species. In this study, we examined the motility, viability (cell membrane integrity) of cryopreserved sperm from three North American acipenseriform species and fertilizing capacity. Milt samples were collected from captive shortnose sturgeon (Acipenser brevirostrum), wild paddlefish (Polyodon spathula) and pallid sturgeon (Scaphirhynchus albus) and cryopreserved using combinations of Modified Tsvetkova's (MT) extender, Original Tsvetkova's extender, and modified Hanks' balanced salt solution, along …

Decline In A Dominant Invertebrate Species Contributes To Altered Carbon Cycling In A Low-Diversity Soil Ecosystem, Byron J. Adams, J. E. Barrett, Ross A. Virginia, Diana H. Wall

Faculty Publications

Low-diversity ecosystems cover large portions of the Earth's land surface, yet studies of climate change on ecosystem functioning typically focus on temperate ecosystems, where diversity is high and the effects of individual species on ecosystem functioning are difficult to determine. We show that a climate-induced decline of an invertebrate species in a low-diversity ecosystem could contribute to significant changes in carbon © cycling. Recent climate variability in the McMurdo Dry Valleys of Antarctica is associated with changes in hydrology, biological productivity, and community composition of terrestrial and aquatic ecosystems. One of the greatest changes documented in the dry valleys is …