Digital Commons Network^™

Watertight Trimmed Nurbs, Thomas W. Sederberg, Xin Li, Hongwei Lin, Heather Ipson

Faculty Publications

This paper addresses the long-standing problem of the unavoidable gaps that arise when expressing the intersection of two NURBS surfaces using conventional trimmed-NURBS representation. The solution converts each trimmed NURBS into an untrimmed T-Spline, and then merges the untrimmed T-Splines into a single, watertight model. The solution enables watertight fillets of NURBS models, as well as arbitrary feature curves that do not have to follow isoparameter curves. The resulting T-Spline representation can be exported without error as a collection of NURBS surfaces.

Prevalence And Infection Load Dynamics Of Rickettsia Felis In Actively Feeding Cat Fleas, Kathryn E. Reif, Rhett W. Stout, Gretchen C. Henry, Lane D. Foil, Kevin R. Macaluso

Faculty Publications

Background: Rickettsia felis is a flea-associated rickettsial pathogen recurrently identified in both colonized and wild-caught cat fleas, Ctenocephalides felis. We hypothesized that within colonized fleas, the intimate relationship between R. felis and C. felis allows for the coordination of rickettsial replication and metabolically active periods during flea bloodmeal acquisition and oogenesis. Methodology/Principal Findings: a quantitative real-time PCR assay was developed to quantify R. felis in actively feeding R. felis-infected fleas. In three separate trials, fleas were allowed to feed on cats, and a mean of 3.9 × 106 R. felis 17-kDa gene copies was detected for each flea. A distinct …

Size And Heterozygosity Influence Partner Selection In The Formosan Subterranean Termite, Claudia Husseneder, Dawn M. Simms

Faculty Publications

In monogamous species that exhibit extensive biparental investment, such as termites, both sexes are predicted to be selective when choosing a mate. Size-related traits are expected to be important in partner selection because the fat reserves of the colony founders sustain the incipient colony. Partner relatedness and heterozygosity determine the degree of inbreeding and genetic diversity within the colony and may thus also influence partner selection. To test these predictions, we investigated whether phenotypic and genetic traits influence mate choice and/or competitive advantage during pair formation of Formosan subterranean termites, Coptotermes formosanus (Isoptera: Rhinotermitidae). Pair formation in termites normally occurs …

Herpes Simplex Virus Type-1(Hsv-1) Oncolytic And Highly Fusogenic Mutants Carrying The Nv1020 Genomic Deletion Effectively Inhibit Primary And Metastatic Tumors In Mice, Anna Israyelyan, Vladimir N. Chouljenko, Abolghasem Baghian, Andrew T. David, Michael T. Kearney, Konstantin G. Kousoulas

Faculty Publications

Background. The NV1020 oncolytic herpes simplex virus type-1 has shown significant promise for the treatment of many different types of tumors in experimental animal models and human trials. Previously, we described the construction and use of the NV1020-like virus OncSyn to treat human breast tumors implanted in nude mice. The syncytial mutation gKsyn1 (Ala-to-Val at position 40) was introduced into the OncSyn viral genome cloned into a bacterial artificial chromosome using double-red mutagenesis in E. coli to produce the OncdSyn virus carrying syncytial mutations in both gB(syn3) and gK(syn1). Results. The OncdSyn virus caused extensive virus-induced cell fusion in cell …

Leaf Area Index Simulation In Soybean Grown Under Near-Optimal Conditions, T. D. Setiyono, A. Weiss, J. E. Specht, K. G. Cassman, A. Dobermann

Faculty Publications

Different approaches have been used to simulate leaf area index (LAI) in soybean (Glycine max L. Merr). Many of these approaches require genotype-specific calibration procedures. Studies modeling LAI dynamics under optimal growth conditions with yields close to the yield potential of soybean have remained scarce. A sink-driven approach was developed and evaluated for LAI simulation in soybean under near-optimal environments. The rate of change in expanding leaf area was simulated using the first derivative of a logistic function accounting for plant population density, air temperature, and water deficit. The rate of change in senescing leaf area was also simulated using …

Tnfα Sirna Reduces Brain Tnf And Eeg Delta Wave Activity In Rats, Ping Taishi, Lynn Churchill, Mingxiang Wang, Daniel Kay, Christopher J. Davis, Xin Guan, Alok De, Tadanobu Yasuda, Fan Liao, James M. Krueger

Faculty Publications

Tumor necrosis factor alpha (TNFα) is a pleiotropic cytokine with several CNS physiological and pathophysiological actions including sleep, memory, thermal and appetite regulation. Short interfering RNAs (siRNA) targeting TNFα were incubated with cortical cell cultures and microinjected into the primary somatosensory cortex (SSctx) of rats. The TNFα siRNA treatment specifically reduced TNFα mRNA by 45% in vitro without affecting interleukin-6 or gluR1-4 mRNA levels. In vivo the TNFα siRNAα reduced TNFα mRNA, interleukin-6 mRNA and gluR1 mRNA levels compared to treatment with a scrambled control siRNA. After in vivo microinjection, the density of TNFα-immunoreactive cells in layer V of the …

The Ethics Of Driving, Amy E. Marga

Faculty Publications

The concept of “neighbor” takes on entirely new meanings in our mobile society. Who becomes my neighbor when I am behind the wheel? What is the consequence of my driving to my neighbors around the globe? What about the impact on our nonhuman “neighbors”? The ethics of driving has many dimensions for the Christian person of goodwill.

Two-Photon Imaging Of Spatially Extended Neuronal Network Dynamics With High Temporal Resolution., Lillis Kp, Eng A, White Ja, Mertz J

Faculty Publications

No abstract provided.

Frequency-Dependent Glycinergic Inhibition Modulates Plasticity In Hippocampus., Keck T, Lillis Kp, Zhou Yd, White Ja

Faculty Publications

No abstract provided.

Victorian Tort Liability For Workplace Injuries, Michael Ashley Stein

Faculty Publications

The first decision of an injured worker suing his master for a workplace accident was reported in 1837, the year of Queen Victoria's ascension. The second Workman's Compensation Act, a comprehensive social insurance scheme, was passed in 1900, a few months before her death. The Article provides an initial account of the development of employers' liability to their servants for work-related injuries during the Victorian era. It demonstrates that English judges, and especially the Barons of the Exchequer, interpreted the law to resist employers' liability. The means these judges used included creating the defence of common employment, widely applying the …

Cu/Zn Superoxide Dismutases In Developing Cotton Fibers: Evidence For An Extracellular Form, Hee Jin Kim, Naohiro Kato, Sunran Kim, Barbara Triplett

Faculty Publications

Hydrogen peroxide and other reactive oxygen species are important signaling molecules in diverse physiological processes. Previously, we discovered superoxide dismutase (SOD) activity in extracellular protein preparations from fiber-bearing cotton (Gossypium hirsutum L.) seeds. We show here, based on immunoreactivity, that the enzyme is a Cu/Zn-SOD (CSD). Immunogold localization shows that CSD localizes to secondary cell walls of developing cotton fibers. Five cotton CSD cDNAs were cloned from cotton fiber and classified into three subfamilies (Group 1: GhCSD1; Group 2: GhCSD2a and GhCSD2b; Group 3: GhCSD3 and GhCSD3s). Members of Group 1 and 2 are expressed throughout fiber development, but predominant …

Effect Of Shilianhua Extract And Its Fractions On Body Weight Of Obese Mice, Jun Yin, Aamir Zuberi, Zhanguo Gao, Dong Liu, Zhijun Liu, William T. Cefalu, Jianping Ye

Faculty Publications

Five commercial botanical products (Shilianhua [SLH] tablets, Shiu Huo pills, Fenulyn, Bitter Melon, and Glucose Metabolic Support), available in the US market, with reported claims for regulation of metabolism were screened for their effect on body weight gain in high-fat diet-induced obese mice. Pilot results suggested that SLH tablets attenuated body weight gain, whereas Shiu Huo pills and Fenulyn tended to promote weight gain in the mice on the high-fat diet. To investigate the bioactive components in the SLH tablet, the wild SLH plant (Sinocrassula indica Berge) was collected from China and used to make a variety of extracts including …

Botanicals As Epigenetic Modulators For Mechanisms Contributing To Development Of Metabolic Syndrome, Heather Kirk, William T. Cefalu, David Ribnicky, Zhijun Liu, Kenneth J. Eilertsen

Faculty Publications

Epigenetics refers to heritable changes in gene expression that are not attributable to changes in DNA sequence and impacts many areas of applied and basic biology including developmental biology, gene therapy, somatic cell nuclear transfer, somatic cell reprogramming, and stem cell biology. Epigenetic changes are known to contribute to aging in addition to multiple disease states. Epigenetic changes can be influenced by environmental factors that in turn can be inherited by daughter cells during cell division and can also be inherited through the germ line. Thus, it is intriguing to consider that epigenetics, in general, may play a role in …

The "Middle Ground" Perspective On The Expropriation Of Indian Lands, Eric Kades

Faculty Publications

No abstract provided.

Doing More Or Doing Less For The Environment: Shedding Light On Epa's "Stealth" Method Of Environmental Enforcement, Ronald H. Rosenberg

Faculty Publications

Since the 1970s, environmental protection goals have gone from general statements of political desire to highly articulated systems of environmental regulation implemented by federal, state, and local governments. Environmental statutes have been enacted giving administrative agencies such as the U.S. Environmental Protection Agency (EPA) the responsibility for translating broad policy goals into specific regulatory requirements. Through its enforcement program, EPA seeks to assure that these general goals are achieved by individual actors. This Article examines a recent trend in EPA's practices, increased reliance on internal agency methods of enforcement. The study analyzes EPA's administrative enforcement system with particular emphasis on …

Understanding (Dis)Abilities Through Children's Literature, Stephanie A. Kurtts, Karen W. Gavigan

Faculty Publications

The authors of this article examined how pre-service teachers can use children's and young adult literature about disabilities to enhance understanding of individual differences through a bibliotherapeutic approach. An introduction to bibliotherapy is provided along with related literature from the field. Strategies for using children's and young adult literature to enhance the understanding of issues associated with disabilities are presented along with one teacher candidate's application of the literature in her classroom. The authors have also included a selected bibliography of children's literature on disabilities as well as relevant websites

A Review Of Fibroblast Populated Collagen Lattices, J. C. Dallon, Paul H. Ehrlich

Faculty Publications

Bellaes introduction of the fibroblast populated collagen lattice (FPCL) (1) has facilitated the study of collagen-cell interactions. As a result of the numerous modifications of the casting of FPCL's, the in vivo applications of these in vitro findings has been confusing. Here experimental FPCL contraction findings are viewed in regard to three proposed mechanisms responsible for lattice contraction. The cellular mechanisms responsible for generating FPCL contraction are: cell contraction, cell tractional forces related to cell locomotion, and initial cell elongation and spreading.

Data-Driven Programming And Behavior For Autonomous Virtual Characters, Jonathan Dinerstein, Parris K. Egbert, Michael A. Goodrich, Dan A. Ventura

Faculty Publications

In the creation of autonomous virtual characters, two levels of autonomy are common. They are often called motion synthesis (low-level autonomy) and behavior synthesis (high-level autonomy), where an action (i.e. motion) achieves a short-term goal and a behavior is a sequence of actions that achieves a long-term goal. There exists a rich literature addressing many aspects of this general problem (and it is discussed in the full paper). In this paper we present a novel technique for behavior (high-level) autonomy and utilize existing motion synthesis techniques. Creating an autonomous virtual character with behavior synthesis abilities frequently includes three stages: forming …

The Western Arctic Linkage Experiment (Wale): Overview And Synthesis, A. D. Mcguire, J. E. Walsh, J. S. Kimball, J. S. Clein, S. E. Euskirchen, S. Drobot, U. C. Herzfeld, J. Maslanik, Richard B. Lammers, M. A. Rawlins, J. Maslanik

Faculty Publications

The primary goal of the Western Arctic Linkage Experiment (WALE) was to better understand uncertainties of simulated hydrologic and ecosystem dynamics of the western Arctic in the context of 1) uncertainties in the data available to drive the models and 2) different approaches to simulating regional hydrology and ecosystem dynamics. Analyses of datasets on climate available for driving hydrologic and ecosystem models within the western Arctic during the late twentieth century indicate that there are substantial differences among the mean states of datasets for temperature, precipitation, vapor pressure, and radiation variables. Among the studies that examined temporal trends among the …

Multidimensional Fluorescence Microscopy Of Multiple Organelles In Arabidopsis Seedlings, Naohiro Kato, Dexter Reynolds, Matthew L. Brown, Marietta Boisdore, Yukichi Fujikawa, Andrea Morales, Lee A. Meisel

Faculty Publications

Background. The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. In planta analyses of this dynamic process have typically been limited to only two organelles or proteins at a time in only a few cell types. Results. We generated a transgenic Arabidopsis plant that contains four spectrally different fluorescent proteins. Nuclei, plastids, mitochondria and plasma membranes were genetically tagged with cyan, red, yellow and green fluorescent proteins, respectively. In addition, methods to track nuclei, mitochondria and chloroplasts and quantify …

Global N Removal By Freshwater Aquatic Systems Using A Spatially Distributed, Within‐Basin Approach, Wilfred M. Wollheim, Charles J. Vorosmarty, A. F. Bouwman, Pamela Green, John Harrison, Ernst Linder, Bruce J. Peterson, Sybil P. Seitzinger, James P. M. Syvitski

Faculty Publications

We explored the role of aquatic systems in the global N cycle using a spatially distributed, within‐basin, aquatic nitrogen (N) removal model, implemented within the Framework for Aquatic Modeling in the Earth System (FrAMES‐N). The model predicts mean annual total N (TN) removal by small rivers (with drainage areas from 2.6–1000 km2), large rivers, lakes, and reservoirs, using a 30′ latitude × longitude river network to route and process material from continental source areas to the coastal zone. Mean annual aquatic TN removal (for the mid‐1990s time period) is determined by the distributions of aquatic TN inputs, mean annual hydrological …

Heart Rate Variability Characteristics In Sedentary Postmenopausal Women Following Six Months Of Exercise Training: The Drew Study, Conrad P. Earnest, Carl J. Lavie, Steven N. Blair, Timothy S. Church

Faculty Publications

Background: Decreased heart rate variability (HRV) is associated with a higher risk of mortality. Overall, postmenopausal women have lower levels of HRV than premenopausal women, which may be additionally complicated by lifestyle related behaviors such as physical inactivity and obesity. Though cardiorespiratory exercise training increases HRV, little is known regarding the exercise dose necessary to promote improvement.

Methodology/Principle Findings: Our primary aim was to measure HRV in post-menopausal women following 6-moths of exercise training. We examined supine resting HRV in 373 post-menopausal women (45-75 y) after 6-months of randomly assigned and double-blinded administered exercise training exercise training at 50%, 100% …

Climate Change Education And The Ecological Footprint, E. Cordero, Anne Marie Todd, D. Abellerra

Faculty Publications

No abstract provided.

Mangrove Production And Carbon Sinks: A Revision Of Global Budget Estimates, Steven Bouillon, Alberto V. Borges, Edward Castañeda-Moya, Karen Diele, Thorsten Dittmar, Norman C. Duke, Erik Kristensen, Shing Y. Lee, Cyril Marchand, Jack J. Middelburg, Victor H. Rivera-Monroy, Thomas J. Smith, Robert R. Twilley

Faculty Publications

Mangrove forests are highly productive but globally threatened coastal ecosystems, whose role in the carbon budget of the coastal zone has long been debated. Here we provide a comprehensive synthesis of the available data on carbon fluxes in mangrove ecosystems. A reassessment of global mangrove primary production from the literature results in a conservative estimate of ∼-218 ± 72 Tg C a^-1. When using the best available estimates of various carbon sinks (organic carbon export, sediment burial, and mineralization), it appears that >50% of the carbon fixed by mangrove vegetation is unaccounted for. This unaccounted carbon sink is …

Evaluation Of Different Insecticides And Fabric Types For Development Of Treated Targets For Stable Fly (Diptera: Muscidae) Control, Jerome A. Hogsette, Alyce Nalli, Lane D. Foil

Faculty Publications

Stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), once only a pest of pastured cattle, has become a serious pest of range cattle in the United States. Because of the difficulties associated with stable fly management under range conditions, a pesticide-impregnated cloth target is being developed as a management tool. We conducted studies to determine the influence of weather, time, fabric type, insecticide type, and insecticide concentration on the mortality of stable flies from a susceptible laboratory colony exposed for 30 s to treated cloth targets. We found that 100% of the flies exposed to trigger (Trigger-Royal Box, 65% polyester and …

Identification Of Rickettsia Felis In The Salivary Glands Of Cat Fleas, Kevin R. Macaluso, Walairat Pornwiroon, Vsevolod L. Popov, Lane D. Foil

Faculty Publications

Rickettsia felis, a flea-associated rickettsial pathogen, has been identified in many tissues, including the digestive and reproductive tissues, within the cat flea, Ctenocephalides felis. We utilized transmission electron microscopy and polymerase chain reaction to identify R. felis in the salivary glands of fed fleas and further define the distribution of R. felis within the arthropod host. We identified Rickettsia-like organisms in salivary glands using electron microscopy. Sequence analysis of portions of the Rickettsia genus-specific 17-kDa antigen gene and R. felis plasmid confirmed the morphological identification of R. felis in cat flea salivary glands. This is the first report of R. …

Mitochondria In Energy-Limited States: Mechanisms That Blunt The Signaling Of Cell Death, Steven C. Hand, Michael A. Menze

Faculty Publications

Cellular conditions experienced during energy-limited states - elevated calcium, shifts in cellular adenylate status, compromised mitochondrial membrane potential - are precisely those that trigger, at least in mammals, the mitochondrion to initiate opening of the permeability transition pore, to assemble additional protein release channels, and to release pro-apoptotic factors. These proapototic factors in turn activate initiator and executer caspases. How is activation of mitochondria-based pathways for the signaling of apoptotic and necrotic cell death avoided under conditions of hypoxia, anoxia, diapause, estivation and anhydrobiosis? Functional trade-offs in environmental tolerance may have occurred in parallel with the evolution of diversified pathways …

Preparation Of Botanical Samples For Biomedical Research, Zhijun Liu

Faculty Publications

Plants are chemical storehouses, a fact which has driven countless multidisciplinary quests for bioactive compounds. As the very first step of botanical research, the whole desire is to find "hit" plants with specific bioactivities. It is logical to use some strategies that can maximize the chances of finding these "hits" with limited time and resources. In addition to selecting the right plants for screening, how the plant extracts are prepared can also influence the bioactivity screening outcomes. An extract from the same plant material can be quite different in chemical composition having different preparations. Because of the complex mixture nature …

The Development Of Day-Night Differences In Sleep And Wakefulness In Norway Rats And The Effect Of Bilateral Enucleation, Andrew J. Gall, William D. Todd, Baisali Ray, Cassandra M. Coleman, Mark S. Blumberg

Faculty Publications

The suprachiasmatic nucleus (SCN) exhibits circadian rhythmicity in fetal and infant rats, but little is known about the consequences of this rhythmicity for infant behavior. Here, in Experiment 1, we measured sleep and wakefulness in rats during the day and night in postnatal day (P)2, P8, P15 and P21 subjects. As early as P2, day-night differences in sleep-wake activity were detected. Nocturnal wakefulness began to emerge around P15 and was reliably expressed by P21. We hypothesized that the process of photic entrainment over the first postnatal week, which depends upon the development of connectivity between the retinohypothalamic tract (RHT) and …

The Architecture Of Instructional Theory, Andrew S. Gibbons, Clint P. Rogers

Faculty Publications

This chapter joins a discussion of instructional theory that has been ongoing for nearly a century. It departs in some ways from prior discussions: (1) it considers instructional theory as a species of technological theory rather than as a type of scientific theory, a view expressed more fully elsewhere (Gibbons, 2003a), (2) it adopts the viewpoint articulated in earlier chapters of this book that there are multiple distinct bodies of technological theory that pertain to the work of instructional designers, (3) it attempts to articulate a particular view of the nature of two of those bodies of theory by describing …