Digital Commons Network^™

Differential Effects Of Akt Isoforms On Somatic Cell Reprogramming, Yong Tang, Zongliang Jiang, Yan Luo, Xueming Zhao, Ling Wang, Carol Norris, Xiuchun Cindy Tian

Faculty Publications

© 2014. Akt plays an important role in cell growth, proliferation and survival. The specific roles of the three Akt isoforms in somatic cell reprogramming have not been investigated. Here we report that, during iPSC generation, enhanced Akt1 activity promotes complete reprogramming mainly through increased activation of Stat3 in concert with leukemia inhibitory factor (LIF) and, to a lesser extent, through promotion of colony formation. Akt1 augments Stat3 activity through activation of mTOR and upregulation of LIF receptor expression. Similarly, enhanced Akt2 or Akt3 activation also promotes reprogramming and coordinates with LIF to activate Stat3. Blocking Akt1 or Akt3 but …

Phenylalanine Residues At The Carboxyl Terminus Of The Herpes Simplex Virus 1 Ul20 Membrane Protein Regulate Cytoplasmic Virion Envelopment And Infectious Virus Production, Anu Susan Charles, Vladimir N. Chouljenko, Nithya Jambunathan, Ramesh Subramanian, Peter Mottram, Konstantin G. Kousoulas

Faculty Publications

The herpes simplex virus type 1 (HSV-1) UL20 gene encodes a 222-amino-acid nonglycosylated envelope protein which forms a complex with viral glycoprotein K (gK) that functions in virion envelopment, egress, and virus-induced cell fusion. To investigate the role of the carboxyl terminus of the UL20 protein (UL20p) in cytoplasmic virion envelopment, a cadre of mutant viruses was constructed and characterized. The deletion of six amino acids from the carboxyl terminus of UL20p caused an approximately 1-log reduction in infectious virus production compared to that of the wild-type virus. Surprisingly, a phenylalanine-toalanine replacement at amino acid position 210 caused a gain-of-function …

Development Of Microsatellites For Population Genetic Analyses Of Tabanus Nigrovittatus (Diptera: Tabanidae), Claudia Husseneder, Jennifer R. Delatte, Jeremy Krumholt, Lane D. Foil

Faculty Publications

ABSTRACT The greenhead horse fly, Tabanus nigrovittatus Macquart (Diptera: Tabanidae), is frequently found in coastal marshes of the Eastern United States. The females are autogenous (i.e., able to develop eggs without a bloodmeal), but they become a considerable pest to both humans and animals when they pursue a source of blood protein to produce additional eggs. In this study, we identified microsatellite markers to provide first insight into the population genetic structure of this notorious pest species. Because no prior genomic information was available for T. nigrovittatus,weused direct shotgun pyrosequencing technology to characterize microsatellite loci. Approximately 10% of the 105,634 …

Gastrointestinal Helminths In Two Species Of Geckos, Gekko Vittatus, Gehyra Oceanica, And Five Species Of Skinks, Caledoniscincus Atropunctatus, Emoia Cyanogaster, Emoia Erronan, Emoia Nigra, Emoia Sanfordi (Squamata) From The Republic Of Vanuatu, Oceania, Stephen R. Goldberg, Charles R. Bursey, Alison M. Hamilton, Christopher C. Austin

Faculty Publications

Two species of geckos, Gekko vittatus, Gehyra oceanica and 5 species of skinks, Caledoniscincus atropunctatus, Emoia cyanogaster, Emoia erronan, Emoia nigra, and Emoia sanfordi from the Republic of Vanuatu were examined for helminths. One species of Digenea (Mesocoelium microon), 3 species of Cestoda (Gekkotaenia novaeguineaensis, Cylindrotaenia hickmani, Ophiotaenia greeri), and 7 species of Nematoda, gravid individuals of Hedruris hanleyae, Maxvachonia chabaudi, Parapharyngodon maplestoni, Spauligodon gehyrae, Spauligodon zweifeli, juveniles assigned to the Filarioidea, and larvae of Physocephalus sp. in cysts were found. Eighteen new host records and 7 new locality records are reported. The lizards of Vanuatu are infected by generalist …

Parental Care In The Cuatro Ciénegas Cichlid, Herichthys Minckleyi (Teleostei: Cichlidae), Ronald G. Oldfield, Kapil Mandrekar, M. Xavier Nieves, Dean A. Hendrickson, Prosanta Chakrabarty, Brook O. Swanson, Hans A. Hofmann

Faculty Publications

© 2014, Springer International Publishing Switzerland. Behavioral studies have often examined parental care by measuring phenotypic plasticity of behavior within a species. Phylogenetic studies have compared parental care among species, but only at broad categories (e.g., care vs. no care). Here we provide a detailed account that integrates phylogenetic analysis with quantitative behavioral data to better understand parental care behavior in the Cuatro Ciénegas cichlid, Herichthys minckleyi. We found that H. minckleyi occurs in a clade of sexually monochromatic or weakly dichromatic monogamous species, but that male and female H. minckleyi have dramatically different reproductive coloration patterns, likely as a …

Adaptation To Ephemeral Habitat May Overcome Natural Barriers And Severe Habitat Fragmentation In A Fire-Dependent Species, The Bachman's Sparrow (Peucaea Aestivalis), Blain Cerame, James A. Cox, Robb T. Brumfield, James W. Tucker, Sabrina S. Taylor

Faculty Publications

Bachman's Sparrow (Peucaea aestivalis) is a fire-dependent species that has undergone range-wide population declines in recent decades. We examined genetic diversity in Bachman's Sparrows to determine whether natural barriers have led to distinct population units and to assess the effect of anthropogenic habitat loss and fragmentation. Genetic diversity was examined across the geographic range by genotyping 226 individuals at 18 microsatellite loci and sequencing 48 individuals at mitochondrial and nuclear genes. Multiple analyses consistently demonstrated little genetic structure and high levels of genetic variation, suggesting that populations are panmictic. Based on these genetic data, separate management units/subspecies designations or translocations …

Using Measures Of Risk Perception To Predict Information Security Behavior: Insights From Electroencephalography (Eeg), Anthony Vance, Bonnie Anderson, C. Brock Kirwan, David Eargle

Faculty Publications

Users' perceptions of risks have important implications for information security, as the actions of individual users can compromise entire systems. Therefore, there is a critical need to understand how users perceive and respond to information security risks. Previous research on perceptions of information security risk has chiefly relied on self-reported measures. Although these studies are valuable, risk perceptions are often associated with feelings—such as fear or doubt—that are difficult to measure accurately using survey instruments. Additionally, it is unclear how these self-reported measures map to actual security behavior. This paper contributes by demonstrating that risk-taking behavior is effectively predicted using …

Intellectual Property And Public Health - A White Paper, Ryan Vacca

Faculty Publications

On October 26, 2012, The University of Akron School of Law's Center for Intellectual Property and Technology hosted its Sixth Annual IP Scholars Forum. In attendance were thirteen legal scholars with expertise and an interest in IP and public health who met to discuss problems and potential solutions at the intersection of these fields. This report summarizes this discussion by describing the problems raised, areas of agreement and disagreement between the participants, suggestions and solutions made by participants, and the subsequent evaluations of these suggestions and solutions.

Led by the moderator, participants at the Forum focused generally on three broad …

Food For Thought: Genetically Modified Seeds As De Facto Standard-Essential Patents, Ryan Vacca, Benjamin M. Cole, Brent J. Horton

Faculty Publications

For several years, courts have improperly calculated damages in cases involving the unlicensed use of genetically modified (GM) seed technology. In particular, when courts determine patent damages based on the hypothetical negotiation method, they err in exaggerating these damages to a point where no rational negotiator would agree. In response, we propose a limited affirmative defense of an implied license due to the patent's status as a de facto standard-essential patent. To be classified as a de facto standard-essential patent, the farmer must prove three elements that reflect the peculiarities of GM seeds used in farming: (1) dominance, (2) impracticability, …

Antibiotic Lock Therapy: Review Of Technique And Logistical Challenges, Julie Ann Justo, P Brandon Bookstaver

Faculty Publications

Antibiotic lock therapy (ALT) for the prevention and treatment of catheter-related bloodstream infections is a simple strategy in theory, yet its real-world application may be delayed or avoided due to technical questions and/or logistical challenges. This review focuses on these latter aspects of ALT, including preparation information for a variety of antibiotic lock solutions (ie, aminoglycosides, beta-lactams, fluoroquinolones, folate antagonists, glycopeptides, glycylcyclines, lipopeptides, oxazolidinones, polymyxins, and tetracyclines) and common clinical issues surrounding ALT administration. Detailed data regarding concentrations, additives, stability/compatibility, and dwell times are summarized. Logistical challenges such as lock preparation procedures, use of additives (eg, heparin, citrate, or ethylenediaminetetraacetic …

Artemisia Extracts Activate Pparγ, Promote Adipogenesis, And Enhance Insulin Sensitivity In Adipose Tissue Of Obese Mice, Allison J. Richard, Thomas P. Burris, David Sanchez-Infantes, Yongjun Wang, David M. Ribnicky, Jacqueline M. Stephens

Faculty Publications

Objective: Studies have shown that the inability of adipose tissue to properly expand during the obese state or respond to insulin can lead to metabolic dysfunction. Artemisia is a diverse group of plants that has a history of medicinal use. The aim of this study was to examine the ability of ethanolic extracts of Artemisia scoparia (SCO) and Artemisia santolinifolia (SAN) to modulate adipocyte development in cultured adipocytes and white adipose tissue (WAT) function invivo using a mouse model of diet-induced obesity. Method: Adipogenesis was assessed using Oil Red O staining and immunoblotting. A nuclear receptor specificity assay was used …

Spatiotemporal Expression Of Trpm4 In The Mouse Cochlea, Mayumi Sakuraba, Junko Murata, Ryoichi Teruyama, Kazusaku Kamiya, Junji Yamaguchi, Hideyuki Okano, Yasuo Uchiyama, Katsuhisa Ikeda

Faculty Publications

The present study was conducted to elucidate the presence of the transient receptor potential cation channel subfamily M member 4, TRPM4, in the mouse inner ear. TRPM4 immunoreactivity (IR) was found in the cell body of inner hair cells (IHCs) in the organ of Corti in the apical side of marginal cells of the stria vascularis, in the apical portion of the dark cells of the vestibule, and in a subset of the type II neurons in the spiral ganglion. Subsequently, changes in the distribution and expression of TRPM4 in the inner ear during embryonic and postnatal developments were also …

Split Luciferase Complementation Assay To Detect Regulated Protein-Protein Interactions In Rice Protoplasts In A Large-Scale Format, Yukichi Fujikawa, Takahiro Nakanishi, Hiroko Kawakami, Kanako Yamasaki, Masa H. Sato, Hiroyuki Tsuji, Makoto Matsuoka, Naohiro Kato

Faculty Publications

© 2014 Fujikawa et al. Background: The rice interactome, in which a network of protein-protein interactions has been elucidated in rice, is a useful resource to identify functional modules of rice signal transduction pathways. Protein-protein interactions occur in cells in two ways, constitutive and regulative. While a yeast-based high-throughput method has been widely used to identify the constitutive interactions, a method to detect the regulated interactions is rarely developed for a large-scale analysis. Results: A split luciferase complementation assay was applied to detect the regulated interactions in rice. A transformation method of rice protoplasts in a 96-well plate was first …

Social Status Differences Regulate The Serotonergic System Of A Cichlid Fish, Astatotilapia Burtoni, Jasmine L. Loveland, Natalie Uy, Karen P. Maruska, Russ E. Carpenter, Russell D. Fernald

Faculty Publications

© 2014. Published by The Company of Biologists Ltd. Serotonin (5-HT) inhibits aggression and modulates aspects of sexual behaviour in many species, but the mechanisms responsible are not well understood. Here, we exploited the social dominance hierarchy of Astatotilapia burtoni to understand the role of the serotonergic system in long-term maintenance of social status. We identified three populations of 5-HT cells in dorsal and ventral periventricular pretectal nuclei (PPd, PPv), the nucleus of the paraventricular organ (PVO) and raphe. Dominant males had more 5-HT cells than subordinates in the raphe, but the size of these cells did not differ between …

The Arabidopsis Siamese-Related Cyclin-Dependent Kinase Inhibitors Smr5 And Smr7 Regulate The Dna Damage Checkpoint In Response To Reactive Oxygen Species, Dalong Yi, Claire Lessa Alvim Kamei, Toon Cools, Sandy Vanderauwera, Naoki Takahashi, Yoko Okushima, Thomas Eekhout, Kaoru Okamoto Yoshiyama, John Larkin, Hilde Van Den Daele, Phillip Conklin, Anne Britt, Masaaki Umeda, Lieven De Veylder

Faculty Publications

Whereas our knowledge about the diverse pathways aiding DNA repair upon genome damage is steadily increasing, little is known about the molecular players that adjust the plant cell cycle in response to DNA stress. By a meta-analysis of DNA stress microarray data sets, three family members of the SIAMESE/SIAMESE-RELATED (SIM/SMR) class of cyclin-dependent kinase inhibitors were discovered that react strongly to genotoxicity. Transcriptional reporter constructs corroborated specific and strong activation of the three SIM/SMR genes in the meristems upon DNA stress, whereas overexpression analysis confirmed their cell cycle inhibitory potential. In agreement with being checkpoint regulators, SMR5 and SMR7 knockout …

Myo-Inositol And Phytate Are Toxic To Formosan Subterranean Termites (Isoptera: Rhinotermitidae), Lucas Veillon, Jared Bourgeois, Amanda Leblanc, Gregg Henderson, Brian D. Marx, Syed Muniruzzaman, Roger A. Laine

Faculty Publications

© 2014 Entomological Society of America. Several rare and common monosaccharides were screened for toxic effects on the Formosan subterranean termite, Coptotermes formosanus Shiraki, with the aim of identifying environmentally friendly termiticides. myo-Inositol and phytic acid, which are nontoxic to mammals, were identified as potential termite control compounds. Feeding bioassays with termite workers, where both compounds were supplied on filter paper in concentrations from 160.2 to 1,281.7μg/mm3, showed concentration-dependent toxicity within 2 wk. Interestingly myo-inositol was nontoxic when administered to termites in agar (40 mg/ml) in the absence of a cellulosic food source, an unexplained phenomenon. In addition, decreased populations …

Strong Natural Selection On Juveniles Maintains A Narrow Adult Hybrid Zone In A Broadcast Spawner, Carlos Prada, Michael E. Hellberg

Faculty Publications

© 2014 by The University of Chicago. Natural selection can maintain and help form species across different habitats, even when dispersal is high. Selection against inferior migrants (immigrant inviability) acts when locally adapted populations suffer high mortality on dispersal to unsuitable habitats. Habitat-specific populations undergoing divergent selection via immigrant inviability should thus show (1) a change in the ratio of adapted to nonadapted individuals among age/size classes and (2) a cline (defined by the environmental gradient) as selection counterbalances migration. Here we examine the frequencies of two depthsegregated lineages in juveniles and adults of a Caribbean octocoral, Eunicea flexuosa. Distributions …

Evaluating Summary Statistics Used To Test For Incomplete Lineage Sorting: Mito-Nuclear Discordance In The Reef Sponge Callyspongia Vaginalis, Melissa B. Debiasse, Bradley J. Nelson, Michael E. Hellberg

Faculty Publications

Conflicting patterns of population differentiation between the mitochondrial and nuclear genomes (mito-nuclear discordance) have become increasingly evident as multilocus data sets have become easier to generate. Incomplete lineage sorting (ILS) of nucDNA is often implicated as the cause of such discordance, stemming from the large effective population size of nucDNA relative to mtDNA. However, selection, sex-biased dispersal and historical demography can also lead to mito-nuclear discordance. Here, we compare patterns of genetic diversity and subdivision for six nuclear protein-coding gene regions to those for mtDNA in a common Caribbean coral reef sponge, Callyspongia vaginalis, along the Florida reef tract. We …

Latitudinal Variation Of Freeze Tolerance In Intertidal Marine Snails Of The Genus Melampus (Gastropoda: Ellobiidae), A. B. Dennis, S. H. Loomis, M. E. Hellberg

Faculty Publications

Low temperatures limit the poleward distribution of many species such that the expansion of geographic range can only be accomplished via evolutionary innovation. We have tested for physiological differences among closely related species to determine whether their poleward latitudinal ranges are limited by tolerance to cold. We measured lower temperature tolerance (LT50) among a group of intertidal pulmonate snails from six congeneric species and nine locales. Differences in tolerance are placed in the context of a molecular phylogeny based on one mitochondrial (cytochrome oxidase subunit I) and two nuclear (histone 3 and a mitochondrial phosphate carrier protein) markers. Temperate species …

The Hoosier Cavefish, A New And Endangered Species (Amblyopsidae, Amblyopsis) From The Caves Of Southern Indiana, Prosanta Chakrabarty, Jacques A. Prejean, Matthew L. Niemiller

Faculty Publications

We describe a new species of amblyopsid cavefish (Percopsiformes: Amblyopsidae) in the genus Amblyopsis from subterranean habitats of southern Indiana, USA. The Hoosier Cavefish, Amblyopsis hoosieri sp. n., is distinguished from A. spelaea, its only congener, based on genetic, geographic, and morphological evidence. Several morphological features distinguish the new species, including a much plumper, Bibendumlike wrinkled body with rounded fins, and the absence of a premature stop codon in the gene rhodopsin. This is the first new cavefish species described from the United States in 40 years and exemplifies how molecular data can alert us to the presence of otherwise …

Specimen Collection: An Essential Tool, L. A. Rocha, A. Aleixo, G. Allen, F. Almeda, C. C. Baldwin, M. V.L. Barclay, J. M. Bates, A. M. Bauer, F. Benzoni, C. M. Berns, M. L. Berumen, D. C. Blackburn, S. Blum, F. Bolaños, R. C.K. Bowie, R. Britz, R. M. Brown, C. D. Cadena, K. Carpenter, L. M. Ceríaco, P. Chakrabarty, G. Chaves, J. H. Choat, K. D. Clements, B. B. Collette, A. Collins, J. Coyne, J. Cracraft, T. Daniel, M. R. De Carvalho, K. De Queiroz, F. Di Dario, R. Drewes

Faculty Publications

No abstract provided.

Hepatitis E Antibodies In Laboratory Rabbits From 2 Us Vendors, Leslie Birke, Stephania A. Cormier, Dahui You, Rhett W. Stout, Christian Clement, Merlin Johnson, Hilary Thompson

Faculty Publications

We tested laboratory rabbits from 2 US vendors for antibodies against hepatitis E virus (HEV); Seroprevalences were 40% and 50%. Retrospective analysis of an ocular herpes simplex 1 experiment demonstrated that HEV seropositivity had no effect on experiment outcome. HEV probably is widespread in research rabbits, but effects on research remain unknown.

Early-Life Exposure To Combustion-Derived Particulate Matter Causes Pulmonary Immunosuppression, J. Saravia, D. You, P. Thevenot, G. I. Lee, B. Shrestha, S. Lomnicki, S. A. Cormier

Faculty Publications

Elevated levels of combustion-derived particulate matter (CDPM) are a risk factor for the development of lung diseases such as asthma. Studies have shown that CDPM exacerbates asthma, inducing acute lung dysfunction and inflammation; however, the impact of CDPM exposure on early immunological responses to allergens remains unclear. To determine the effects of early-lifeCDPMexposure on allergic asthma development in infants, we exposed infant mice to CDPM and then induced a mouse model of asthma using house dust mite (HDM) allergen. Mice exposed to CDPMHDM failed to develop a typical asthma phenotype including airway hyper-responsiveness, T-helper type 2 (Th2) inflammation, Muc5ac expression, …

Metacom: An R Package For The Analysis Of Metacommunity Structure, Tad Dallas

Faculty Publications

Metacommunity theory is an extension of metapopulation theory with the goal of understanding how ecological communities vary through space and time. One off-shoot of metacommunity theory deals with understanding how community structure varies along biotic or environmental gradients. The Elements of Metacommunity Structure framework is a three-tiered analysis of metacommunity structure that enables the user to identify metacommunity properties that arise in site-by-species incidence matrices. These properties can then be related to underlying variables that influence species distributions. The EMS framework is now implemented in metacom, an open source R package that allows for the analysis and plotting of metacommunities. …

Weight Gain Reveals Dramatic Increases In Skeletal Muscle Extracellular Matrix Remodeling, Charmaine S. Tam, Jeffrey D. Covington, Sudip Bajpeyi, Yourka Tchoukalova, David Burk, Darcy L. Johannsen, Cristina M. Zingaretti, Saverio Cinti, Eric Ravussin

Faculty Publications

Context: In animal models of obesity, chronic inflammation and dysregulated extracellular matrix remodeling in adipose tissue leads to insulin resistance. Whether similar pathophysiology occurs in humans is not clear. Objective: The aim of this study was to test whether 10% weight gain induced by overfeeding triggers inflammation and extracellular matrix remodeling (gene expression, protein, histology) in skeletal muscleandsc adipose tissue in humans.Wealso investigated whether such remodelingwas associated with an impaired metabolic response (hyperinsulinemic-euglycemic clamp). Design, Setting, Participants, and Intervention: Twenty-nine free-living males were fed 40% over their baseline energy requirements for 8 weeks. Results: Ten percent body weight gain prompted …

Functional Characterization Of Chemosensory Proteins In The Scarab Beetle, Holotrichia Oblita Faldermann (Coleoptera: Scarabaeida), Hongyan Sun, Li Guan, Honglin Feng, Jiao Yin, Yazhong Cao, Jinghui Xi, Kebin Li

Faculty Publications

Chemosensory proteins (CSPs) play important roles in chemical communication by insects, as they recognize and transport environmental chemical signals to receptors within sensilla. In this study, we identified HoblCSP1 and HoblCSP2 from a cDNA library of Holotrichia oblita antennae, successfully expressed them in E. coli and purified them by Ni ion affinity chromatography. We then measured the ligand-binding specificities of HoblCSP1 and Hobl CSP2 to 50 selected ligands in a competitive binding assay. These results demonstrated that HoblCSP1 and HoblCSP2 have similar ligand-binding spectra. Both proteins displayed the highest affinity for β-ionone, α-ionone and cinnamaldehyde, indicating that they prefer binding …

Potential Use Of An Essential Oil From The Flower Of Swertia Densifolia As A Repellent For Apis Florea (Hymenoptera: Apidae), D. G. Naik, H. Vaidya-Kannur, P. V. Deshpande, C. N. Dandge, G. V.P. Reddy

Faculty Publications

ABSTRACT Formulations of compounds repellent to honey bees may be useful tools to keep bees away from areas recently treated with toxic insecticides. Here, we report the activity of a previously unknown honey bee repellent, an essential oil from the flower of Swertia densifolia (Gentianaceae), which has activity toward Indian honey bees Apis florea F. The essential oil was obtained by hydrodistillation and was subjected to a rotating table bioassay with foragers of the honey bee A. florea. Repellency was dose-dependent up to 12 mg/ml and remained constant thereafter. The chemical constitution of the essential oil was examined by gas …

Research Capacity. Enabling The Genomic Revolution In Africa, Charles Rotimi, Akin Abayomi, Alash'le Abimiku, Victoria May Adabayeri, Clement Adebamowo, Ezekiel Adebiyi, Adebowale D. Ademola, Adebowale Adeyemo, Dwomoa Adu, Dissou Affolabi, Godfred Agongo, Samuel Ajayi, Sally Akarolo-Anthony, Rufus Akinyemi, Albert Akpalu, Marianne Alberts, Orlando Alonso Betancourt, Ahmed Mansour Alzohairy, Gobena Ameni, Olukemi Amodu, Gabriel Anabwani, Kristian G. Andersen, Fatiu Arogundade, Oyedunni Arulogun, Danny A. Asogun, Rasheed Bakare, Naby Balde, Mary Lynn Baniecki, Christine Beiswanger, Alia Benkahla, Lara Bethke, Micheal Boehnke, Vincent Boima

Faculty Publications

No abstract provided.

Culture Of Lobotes Surinamensis (Tripletail), Eric Saillant, Jason T. Lemus, James S. Franks

Faculty Publications

No abstract provided.

The Common Marmoset Genome Provides Insight Into Primate Biology And Evolution, Kim C. Worley, Wesley C. Warren, Jeffrey Rogers, Devin Locke, Donna M. Muzny, Elaine R. Mardis, George M. Weinstock, Suzette D. Tardif, Kjersti M. Aagaard, Nicoletta Archidiacono, Nirmala Arul Rayan, Mark A. Batzer, Kathryn Beal, Brona Brejova, Oronzo Capozzi, Saverio B. Capuano, Claudio Casola, Mimi M. Chandrabose, Andrew Cree, Marvin Diep Dao, Pieter J. De Jong, Ricardo Cruz-Herrera Del Rosario, Kim D. Delehaunty, Huyen H. Dinh, Evan E. Eichler, Stephen Fitzgerald, Paul Flicek, Catherine C. Fontenot, R. Gerald Fowler, Catrina Fronick, Lucinda A. Fulton, Robert S. Fulton, Ramatu Ayiesha Gabisi, Daniel Gerlach

Faculty Publications

We report the whole-genome sequence of the common marmoset (Callithrix jacchus). The 2.26-Gb genome of a female marmoset was assembled using Sanger read data (6×) and a whole-genome shotgun strategy. A first analysis has permitted comparison with the genomes of apes and Old World monkeys and the identification of specific features that might contribute to the unique biology of this diminutive primate, including genetic changes that may influence body size, frequent twinning and chimerism. We observed positive selection in growth hormone/insulin-like growth factor genes (growth pathways), respiratory complex I genes (metabolic pathways), and genes encoding immunobiological factors and proteases (reproductive …