Digital Commons Network^™

Scale Dependence Of Sex-Specific Movement In A Small-Bodied Stream Fish, Scott R. Clark, Brian R. Kreiser, Jacob F. Schraefer, Laura K. Stewart

Faculty Publications

1. Animal movement at localised scales is often modulated by competing pressures such as avoiding predators while acquiring resources and mates. The relative magnitude of these trade‐offs may affect males and females differently, often resulting in sex‐specific differences in movement.
2. Sex‐biases in movement have been linked to mating systems (e.g. monogamy or polygamy) in birds and mammals; however, this relationship has received less attention among fishes. Using passive integrated transponder tags and a series of stationary antennas, we evaluated the movement dynamics of a small‐bodied, sexually dimorphic stream fish Fundulus olivaceus over a 30‐day period in a fourth‐order tributary to the …

The Molecular Biogeography Of The Indo-Pacific: Testing Hypotheses With Multispecies Genetic Patterns, Eric D. Crandall, Cynthia Riginos, Chris E. Bird, Libby Liggins, Eric Treml, Maria Beger, Paul H. Barber, Sean R. Connolly, Peter F. Cowman, Joseph D. Dibattista, Jeff A. Eble, Sharon F. Magnuson, John B. Horne, Marc Kochzius, Harilaos A. Lessios, Shang Yin Vanson Liu, William B. Ludt, Hawis Madduppa, John M. Pandolfi, Robert J. Toonen, Michelle R. Gaither

Faculty Publications

Aim: To test hypothesized biogeographic partitions of the tropical Indo-Pacific Ocean with phylogeographic data from 56 taxa, and to evaluate the strength and nature of barriers emerging from this test.

>Location: The Indo-Pacific Ocean.

Time Period: Pliocene through the Holocene.

Major Taxa Studied: Fifty-six marine species.

Methods: We tested eight biogeographic hypotheses for partitioning of the Indo-Pacific using a novel modification to analysis of molecular variance. Putative barriers to gene flow emerging from this analysis were evaluated for pairwise Φ_ST, and these Φ_ST distributions were compared to distributions from randomized datasets and …

Why Nature Matters: Seventh-Day Adventist Education In The Anthropocene, Daniel Gonzalez-Socoloske

Faculty Publications

No abstract provided.

Linking Nutrient Stoichiometry To Zika Virus Transmission In A Mosquito, Andrew S. Paige, Shawna K. Bellamy, Barry W. Alto, Catherine L. Dean, Donald A. Yee

Faculty Publications

Food quality and quantity serve as the basis for cycling of key chemical elements in trophic interactions; yet the role of nutrient stoichiometry in shaping host–pathogen interactions is under appreciated. Most of the emergent mosquito-borne viruses affecting human health are transmitted by mosquitoes that inhabit container systems during their immature stages, where allochthonous input of detritus serves as the basal nutrients. Quantity and type of detritus (animal and plant) were manipulated in microcosms containing newly hatched Aedes aegypti mosquito larvae. Adult mosquitoes derived from these microcosms were allowed to ingest Zika virus-infected blood and then tested for disseminated infection, transmission, …

Fluorescence Hplc Analysis Of The In-Vivo Activity Of Glucosylceramide Synthase, Kartik R. Roy, Sachin K. Khiste, Zhijun Liu, Yong Yu Liu

Faculty Publications

Almost all functions of cells or organs rely on the activities of cellular enzymes. Indeed, the in-vivo activities that directly represent the cellular effects of enzymes in live organs are critical importance to appreciate the roles enzymes play in modulating physiological or pathological processes, although assessments of such in-vivo enzyme activity are more difficult than typical test-tube assays. Recently, we, for the first time, developed a direct and easy-handling method for HPLC analyzing the in-vivo activity of glucosylceramide synthase (GCS). GCS that converts ceramide into glucosylceramide is a limiting-enzyme in the syntheses of glycosphingolipids and is one cause of cancer …

Communication Addressing North Carolina’S Role In The Cia’S Extraordinary Rendition And Torture Program And Request For Coordinated Measures Including State Visit, Investigations, And International Condemnation, Deborah M. Weissman

Faculty Publications

No abstract provided.

Environmental Microcystin Targets The Microbiome And Increases The Risk Of Intestinal Inflammatory Pathology Via Nox2 In Underlying Murine Model Of Nonalcoholic Fatty Liver Disease, Sutapa Sarkar, Diana Kimono, Muayad Albadrani, Ratanesh K. Seth, Philip Busbee, Hasan Alghetaa, Dwayne E. Porter, Geoff I. Scott, Bryan Brooks, Mitzi Nagarkatti, Prakash Nagarkatti, Saurabh Chatterjee

Faculty Publications

With increased climate change pressures likely to influence harmful algal blooms, exposure to microcystin, a known hepatotoxin and a byproduct of cyanobacterial blooms can be a risk factor for NAFLD associated comorbidities. Using both in vivo and in vitro experiments we show that microcystin exposure in NAFLD mice cause rapid alteration of gut microbiome, rise in bacterial genus known for mediating gut inflammation and lactate production. Changes in the microbiome were strongly associated with inflammatory pathology in the intestine, gut leaching, tight junction protein alterations and increased oxidative tyrosyl radicals. Increased lactate producing bacteria from the altered microbiome was associated …

Repurposing Of Glycine-Rich Proteins In Abiotic And Biotic Stresses In The Lone-Star Tick (Amblyomma Americanum), Rebekah Bullard, Surendra Raj Sharma, Pradipta Kumar Das, Sarah E. Morgan, Shahid Karim

Faculty Publications

Tick feeding requires the secretion of a huge number of pharmacologically dynamic proteins and other molecules which are vital for the formation of the cement cone, the establishment of the blood pool and to counter against the host immune response. Glycine-rich proteins (GRP) are found in many organisms and can function in a variety of cellular processes and structures. The functional characterization of the GRPs in the tick salivary glands has not been elucidated. GRPs have been found to play a role in the formation of the cement cone; however, new evidence suggests repurposing of GRPs in the tick physiology. …

Changes In The Spatial Distribution And Anatomy Of A Range Shift For The Atlantic Surfclam Spisula Solidissima In The Mid-Atlantic Bight And On Georges Bank, Jeremy R. Timbs, Eric N. Powell, Roger Mann

Faculty Publications

The Atlantic surfclam Spisula solidissima supports one of the largest fisheries on the US northeast coast. Using ~30 yr of data from surfclam stock surveys, variance-to-mean ratios (VtMRs) were calculated both temporally and spatially for a range of surfclam size classes to determine the degree of patchiness. The VtMR declined from the 1980s to present in all regions (offshore Delmarva, New Jersey, Long Island, Southern New England, Georges Bank); however, VtMR rose with increasing clam size. Taylor’s power law (TPL) analysis corroborated the VtMR; the surfclam is highly patchy across its range. The surfclam’s proclivity for a patchy distribution varied …

Linking Water Quality To Aedes Aegypti And Zika In Flood-Prone Neighborhoods, Susan Harrell Yee, Donald A. Yee, Rebeca De Jesus Crespo, Autumn Oczkowski, Fengwei Bai, Stephanie Friedman

Faculty Publications

The ability of ecosystems to regulate water quality and flood events has been linked to health outcomes, including mosquito-borne illnesses. In the San Juan Bay Estuary watershed of Puerto Rico, habitat alterations and land-use development have disrupted watershed hydrology, exacerbating wastewater discharges and subjecting some neighborhoods to frequent flooding events. In 2016, the mosquito-borne illness Zika became a new cause for concern. We hypothesized that nutrient-enriched flood water could provide pulses of supplemental nutrients to local mosquito populations. We conducted a field study in six neighborhoods adjacent to the estuary to assess whether environmental variability of nutrient inputs could be …

Dietary Inflammatory Index And Sleep Quality In Southern Italian Adults, Justyna Godos, Raffaele Ferri, Filippo Caraci, Filomena I. I. Cosentino, Sabrina Castellano, Nitin Shivappa, James R. Hébert, Fabio Galvano, Giuseppe Grosso

Faculty Publications

Background: Current evidence supports the central role of a subclinical, low-grade inflammation in a number of chronic illnesses and mental disorders; however, studies on sleep quality are scarce. The aim of this study was to test the association between the inflammatory potential of the diet and sleep quality in a cohort of Italian adults. Methods: A cross-sectional analysis of baseline data of the Mediterranean healthy Eating, Aging, and Lifestyle (MEAL) study was conducted on 1936 individuals recruited in the urban area of Catania during 2014-2015 through random sampling. A food frequency questionnaire and other validated instruments were used to calculate …

Habitat Preference Modulates Transoceanic Dispersal In A Terrestrial Vertebrate, Mozes P.K. Blom, Nicholas J. Matzke, Jason G. Bragg, Evy Arida, Christopher C. Austin, Adam R. Backlin, Miguel A. Carretero, Robert N. Fisher, Frank Glaw, Stacie A. Hathaway, Djoko T. Iskandar, Jimmy A. Mcguire, Benjamin R. Karin, Sean B. Reilly, Eric N. Rittmeyer, Sara Rocha, Mickaël Sanchez, Alexander L. Stubbs, Miguel Vences, Craig Moritz

Faculty Publications

© 2019 The Author(s) Published by the Royal Society. All rights reserved. The importance of long-distance dispersal (LDD) in shaping geographical distributions has been debated since the nineteenth century. In terrestrial vertebrates, LDD events across large water bodies are considered highly improbable, but organismal traits affecting dispersal capacity are generally not taken into account. Here, we focus on a recent lizard radiation and combine a summary-coalescent species tree based on 1225 exons with a probabilistic model that links dispersal capacity to an evolving trait, to investigate whether ecological specialization has influenced the probability of trans-oceanic dispersal. Cryptoblepharus species that occur …

Meat Analogs From Different Protein Sources: A Comparison Of Their Sustainability And Nutritional Content, Ujué Fresán, Maximino Alfredo Mejia, Winston J. Craig, Karen Jaceldo-Siegl, Joan Sabaté

Faculty Publications

© 2018 by the authors. Meat analogs are processed foods designed to mimic meat products. Their popularity is increasing among people seeking foods that are healthy and sustainable. Animal-sourced protein products differ in both their environmental impact and nutritional composition. The protein sources to produce meat analogs come from different plants. There is a lack of published research data assessing differences in these two aspects of meat analogs according to the plant protein source. This study compared the greenhouse gas (GHG) emissions of different types of meat analogs according to their main source of protein (wheat, soy, wheat and soy, …

Is Selenoprotein K Required For Borrelia Burgdorferi Infection Within The Tick Vector Ixodes Scapularis?, Deepak Kumar, Monica Embers, Thomas N. Mather, Shahid Karim

Faculty Publications

Background: Tick selenoproteins are involved in regulating oxidative and endoplasmic reticulum stress during prolonged tick feeding on mammalian hosts. How selenoproteins are activated upon tick-borne pathogen infection is yet to be defined.

Methods: To examine the functional role of selenoprotein K in Borrelia burgdorferi infection within the tick host Ixodes scapularis, RNA interference (RNAi)-based gene silencing was performed.

Results: Selenoprotein K is an endoplasmic reticulum (ER)-resident protein and a component of the ERAD complex involved in ER homeostasis. A qRT-PCR assay revealed the significant upregulation of selenogene K (selenoK) expression in B. burgdorferi-infected …

Cellular Effects Of Butyrate On Vascular Smooth Muscle Cells Are Mediated Through Disparate Actions On Dual Targets, Histone Deacetylase (Hdac) Activity And Pi3k/Akt Signaling Network, Omana P. Mathew, Kasturi Ranganna, Joseph Mathew, Meiling Zhu, Zivar Yousefipour, Chelliah Selvam, Shirlette G. Milton

Faculty Publications

Vascular remodeling is a characteristic feature of cardiovascular diseases. Altered cellular processes of vascular smooth muscle cells (VSMCs) is a crucial component in vascular remodeling. Histone deacetylase inhibitor (HDACI), butyrate, arrests VSMC proliferation and promotes cell growth. The objective of the study is to determine the mechanism of butyrate-induced VSMC growth. Using proliferating VSMCs exposed to 5 mM butyrate, immunoblotting studies are performed to determine whether PI3K/Akt pathway that regulates different cellular effects is a target of butyrate-induced VSMC growth. Butyrate inhibits phosphorylation-dependent activation of PI3K, PDK1, and Akt, eliciting differential effects on downstream targets of Akt. Along with previously …

Sexually Dimorphic Oxytocin Receptorexpressing Neurons In The Preoptic Area Of The Mouse Brain, Kaustubh Sharma, Ryan Leblanc, Masudul Haque, Katsuhiko Nishimori, Madigan M. Reid, Ryoichi Teruyama

Faculty Publications

© 2019 Sharma et al. Oxytocin is involved in the regulation of social behaviors including parental behaviors in a variety of species. Oxytocin triggers social behaviors by binding to oxytocin receptors (OXTRs) in various parts of the brain. OXTRs are present in the preoptic area (POA) where hormone-sensitive sexually dimorphic nuclei exist. The present study was conducted to examine whether sex differences exist in the distribution of neurons expressing OXTRs in the POA. Using OXTR-Venus (an enhanced variant of yellow fluorescent protein) mice, the distribution of OXTR-Venus cells in the POA was compared between sexes. The total number of OXTR-Venus …

Contrasting Latitudinal Gradients Of Body Size In Helminth Parasites And Their Hosts, Tad Dallas, Alyssa Lois M. Gehman, A. Alonso Aguirre, Sarah A. Budischak, John M. Drake, Maxwell J. Farrell, Ria Ghai, Shan Huang, Ignacio Morales-Castilla

Faculty Publications

© 2019 John Wiley & Sons Ltd Aim: We examined body size scaling relationships for two developmental life stages of parasitic helminths (egg and adult) separately in relationship to latitude (i.e. Bergmann's rule), temperature and temperature seasonality. Given that helminth eggs experience environmental conditions more directly, whereas adults live inside infected host individuals, we predict stronger environmentally driven gradients of body size for eggs than for adults. Location: Global. Time period: Present day. Major taxa studied: Parasitic helminths. Methods: We compiled egg size and adult body size data (both minimum and maximum) for 265 parasitic helminth species from the literature, …

Broad-Scale Habitat Use By Fledgling Black-Capped Vireo, Marisa T. Martinez, Ashley M. Long, Heather A. Mathewson, Michael L. Morrison

Faculty Publications

Knowledge of avian habitat associations during the postfledging stage is lacking for most songbirds, which limits the ability to develop and implement management plans that address the full life cycle of birds. The Black-capped Vireo (Vireo atricapilla; hereafter vireo) is a Neotropical migratory songbird that breeds in early to mid-successional vegetation but has been documented using late-successional vegetation. We used data collected in 2013 and 2014 to determine the spatial distribution of vireo fledglings in relation to the availability of woodland cover in central Texas. We found that woodland cover was used less often than it was available in the …

Efficacy Of A New Topical Formulation Containing Selamectin Plus Sarolaner Against Three Common Tick Species Infesting Cats In The United States, Adriano F. Vatta, David R. Young, William R. Everett, Vickie L. King, Judith A. Cherni, Marcela Von Reitzenstein, Susan J. Holzmer, Sara Chapin, Douglas Rugg

Faculty Publications

The efficacy of a single topical application of a combination product containing selamectin and sarolaner (selamectin/sarolaner; Revolution® Plus/Stronghold® Plus) was evaluated in seven laboratory studies against Ixodes scapularis (three studies), Dermacentor variabilis (two studies), or Amblyomma maculatum (two studies). In each study, cats were randomly allocated to treatment groups based on pre-treatment host-suitability tick counts. On Days -2, 5, 12, 19, 26 and 33, the cats were infested with unfed adult ticks. On Day 0, cats were treated with either a placebo (vehicle control) or with the spot-on solution at the minimum dose of 6.0 mg selamectin and 1.0 mg …

Becoming Animal: Karma And The Animal Realm Envisioned Through An Early Yogacara Lens, Daniel Malinowski Stuart

Faculty Publications

In an early discourse from the Saṃyuttanikāya, the Buddha states: “I do not see any other order of living beings so diversified as those in the animal realm. Even those beings in the animal realm have been diversified by the mind, yet the mind is even more diverse than those beings in the animal realm.” This paper explores how this key early Buddhist idea gets elaborated in various layers of Buddhist discourse during a millennium of historical development. I focus in particular on a middle period Buddhist sūtra, the Saddharmasmṛtyupasthānasūtra, which serves as a bridge between early Buddhist theories of …

Expenditure, Coping, And Academic Behaviors Among Food-Insecure College Students At 10 Higher Education Institutes In The Appalachian And Southeastern Regions, Rebecca L. Hagedorn, Laura H. Mcarthur, Lanae B. Hood, Maureen Berner, Elizabeth T. Anderson Steeves, Carol L. Connell, Elizabeth Wall-Bassett, Marsha Spence, Oyinlola Toyin Babatunde, E. Brooke Kelly, Julia F. Waity, J. Porter Lillis, Melissa D. Olfert

Faculty Publications

Background

A number of studies have measured college student food insecurity prevalence higher than the national average; however, no multicampus regional study among students at 4-y institutions has been undertaken in the Appalachian and Southeast regions of the United States. Objectives

The aims of this study were to determine the prevalence of food insecurity among college students in the Appalachian and Southeastern regions of the United States, and to determine the association between food-insecurity status and money expenditures, coping strategies, and academic performance among a regional sample of college students. Methods

This regional, cross-sectional, online survey study included 13,642 college …

Current Status And Recommendations Toward A Virus Standard For Ballast Water, Yiseul Kim, Samuel D. Snow, Vanessa Reichel-Deland, Mostafa Maghsoodi, Gabrielle M. Langlois, Volodymyr V. Tarabara, Joan B. Rose

Faculty Publications

Ballast water contains and transfers a variety of biological and non-biological materials between biomes. This study aimed to summarize published literature on the viruses found in ballast water, to evaluate ultrafiltration and plaque assay as methods for concentrating and enumerating viruses, and to assess the efficacy of UV irradiation in the inactivation of infectious viruses in ballast water. Previous studies reported high virus concentrations in ballast tanks, ranging from 109 to 1014 viral particles/L. The ultrafiltration and plaque assay were efficient in concentrating and enumerating four types of phages: MS2, PhiX174, P22, and PP7. The UV dose (radiant fluence) necessary …

Adiposity Mediates The Association Between The Dietary Inflammatory Index And Markers Of Type 2 Diabetes Risk In Middle-Aged Black South African Women, Asanda Mtintsilana, Lisa K. Micklesfield, Elin Chorell, Tommy Olsson, Nitin Shivappa, James R. Hebert, Andre P. Kengne, Julia H. Goedecke

Faculty Publications

The dietary inflammatory index (DII^®), a validated tool used to measure the inflammatory potential of the diet, has been associated with metabolic disorders in various settings, but not in African populations. The aim of this study was to investigate whether the DII is associated with markers of type 2 diabetes (T2D) risk, and if this association is mediated by adiposity and/or low-grade inflammation, in black South Africa women. Energy-adjusted-DII (E-DII) scores were calculated in 190 women (median age, 53 years) from the Birth-to-Twenty plus cohort using a validated food frequency questionnaire. Fasting glucose, insulin, HbA1c, and inflammatory cytokines …

The Kinesin‐3 Motor, Klp‐4, Mediates Axonal Organization And Cholinergic Signaling In Caenorhabditis Elegans, Margaret E. Magaletta, Kendall J. Perkins, Catherine P. Deuchler, Jay N. Pieczynski

Faculty Publications

Microtubule plus‐end directed trafficking is dominated by kinesin motors, yet kinesins differ in terms of cargo identity, movement rate, and distance travelled. Functional diversity of kinesins is especially apparent in polarized neurons, where long distance trafficking is required for efficient signal transduction‐behavioral response paradigms. The Kinesin‐3 superfamily are expressed in neurons and are hypothesized to have significant roles in neuronal signal transduction due to their high processivity. Although much is known about Kinesin‐3 motors mechanistically in vitro, there is little known about their mechanisms in vivo. Here, we analyzed KLP‐4, the Caenorhabditis elegans homologue of human KIF13A and KIF13B. …

Challenges For Natural Hydrogels In Tissue Engineering, Esmaiel Jabbari

Faculty Publications

Protein-based biopolymers derived from natural tissues possess a hierarchical structure in their native state. Strongly solvating, reducing and stabilizing agents, as well as heat, pressure, and enzymes are used to isolate protein-based biopolymers from their natural tissue, solubilize them in aqueous solution and convert them into injectable or preformed hydrogels for applications in tissue engineering and regenerative medicine. This review aims to highlight the need to investigate the nano-/micro-structure of hydrogels derived from the extracellular matrix proteins of natural tissues. Future work should focus on identifying the nature of secondary, tertiary, and higher order structure formation in protein-based hydrogels derived …

Female Family Members Lack Understanding Of Indeterminate Negative Brca1/2 Test Results Shared By Probands, Deborah Himes, Deborah K. Gibbons, Wendy C. Birmingham, Renea L. Beckstrand, Amanda Gammon, Anita Y. Kinney, Margaret F. Clayton

Faculty Publications

Genetic test results have important implications for close family members. Indeterminate negative results are the most common outcome of BRCA1/2 mutation testing. Little is known about family members’ understanding of indeterminate negative BRCA1/2 test results. The purpose of this mixed-methods study was to investigate how daughters and sisters received and understood genetic test results as shared by their mothers or sisters. Participants included 81 women aged 40-74 with mothers or sisters previously diagnosed with breast cancer and who received indeterminate negative BRCA1/2 test results. Participants had never been diagnosed with breast cancer nor received their own genetic testing or counseling. …

Challenges For Natural Hydrogels In Tissue Engineering, Esmaiel Jabbari

Faculty Publications

Protein-based biopolymers derived from natural tissues possess a hierarchical structure in their native state. Strongly solvating, reducing and stabilizing agents, as well as heat, pressure, and enzymes are used to isolate protein-based biopolymers from their natural tissue, solubilize them in aqueous solution and convert them into injectable or preformed hydrogels for applications in tissue engineering and regenerative medicine. This review aims to highlight the need to investigate the nano-/micro-structure of hydrogels derived from the extracellular matrix proteins of natural tissues. Future work should focus on identifying the nature of secondary, tertiary, and higher order structure formation in protein-based hydrogels derived …

Hypermongone C Accelerates Wound Healing Through The Modulation Of Inflammatory Factors And Promotion Of Fibroblast Migration, Sara E. Moghadam, Moridi Mahdi Farimani, Sara Soroury, Samad N. Ebrahimi, Ehsan Jabbarzadeh

Faculty Publications

The physiology of wound healing is dependent on the crosstalk between inflammatory mediators and cellular components of skin regeneration including fibroblasts and endothelial cells. Therefore, strategies to promote healing must regulate this crosstalk to achieve maximum efficacy. In light of the remarkable potential of natural compounds to target multiple signaling mechanisms, this study aims to demonstrate the potential of hypermongone C, a polycyclic polyprenylated acylphloroglucinol (PPAP), to accelerate wound closure by concurrently enhancing fibroblast proliferation and migration, promoting angiogenesis, and suppressing pro-inflammatory cytokines. This compound belongs to a family of plants (Hypericum) that traditionally have been used to treat injuries. …

Contribution Of Maize Polyamine And Amino Acid Metabolism Toward Resistance Against Aspergillus Flavus Infection And Aflatoxin Production., Rajtilak Majumdar, Rakesh Minocha, Matthew D. Lebar, Kanniah Rajasekaran, Stephanie Long, Carol H. Carter-Wientjes, Subhash C. Minocha, Jeffrey W. Cary

Faculty Publications

Polyamines (PAs) are ubiquitous polycations found in plants and other organisms that are essential for growth, development, and resistance against abiotic and biotic stresses. The role of PAs in plant disease resistance depends on the relative abundance of higher PAs [spermidine (Spd), spermine (Spm)] vs. the diamine putrescine (Put) and PA catabolism. With respect to the pathogen, PAs are required to achieve successful pathogenesis of the host. Maize is an important food and feed crop, which is highly susceptible to Aspergillus flavus infection. Upon infection, the fungus produces carcinogenic aflatoxins and numerous other toxic secondary metabolites that adversely affect human …

Non-Canonical Amino Acid Labeling In Proteomics And Biotechnology, Aya M. Saleh, Kristen M. Wilding, Sarah Calve, Bradley Charles Bundy, Tamara L. Kinzer-Ursem

Faculty Publications

Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique bioorthogonal chemical groups during de novo synthesis by taking advantage of both endogenous and heterologous protein synthesis machineries. Labeled proteins can then be selectively conjugated to fluorophores, affinity reagents, peptides, polymers, nanoparticles or surfaces for a wide variety of downstream applications in proteomics and biotechnology. In this review, we focus on techniques in which proteins are residue- and site-specifically labeled with ncAAs containing bioorthogonal handles. These ncAA-labeled proteins are: readily enriched from cells and tissues for identification via mass spectrometry-based proteomic analysis; selectively purified for downstream biotechnology applications; or …