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Identification Of Pathways Associated With Placental Adaptation To Maternal Nutrient Restriction In Sheep, Ashley K. Edwards, Kathrin A. Dunlap, Thomas E. Spencer, M. Carey Satterfield Sep 2020

Identification Of Pathways Associated With Placental Adaptation To Maternal Nutrient Restriction In Sheep, Ashley K. Edwards, Kathrin A. Dunlap, Thomas E. Spencer, M. Carey Satterfield

Faculty Publications

Maternal nutrient restriction impairs placental growth and development, but available evidence suggests that adaptive mechanisms exist, in a subset of nutrient restricted (NR) ewes, that support normal fetal growth and do not result in intrauterine growth restriction (IUGR). This study utilized Affymetrix GeneChip Bovine and Ovine Genome 1.0 ST Arrays to identify novel placental genes associated with differential fetal growth rates within NR ewes. Singleton pregnancies were generated by embryo transfer and, beginning on Day 35 of pregnancy, ewes received either a 100% NRC (control-fed group; n = 7) or 50% NRC (NR group; n = 24) diet until necropsy …

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Synergisms In Science: Climate Change And Integrated Pest Management Through The Lens Of Communication-2019 Student Debates, Jocelyn R. Holt, Lina Bernaola, Kadie E. Britt, Chirs Mccullough, Morgan Roth, Jennie Wagner, Max Ragozzino, Leslie Aviles, Zhilin Li, Forest Huval, Manoj Pandey, Benjamin W. Lee, Megan Asche, Abigail Hayes, Abigail Cohen, Adrian Marshall, Hannah E. Quellhorst, Rachel V. Wilkins, Valerie Nguyen, Jacqueline Maille, Rachel K. Skinner, John J. Ternest, Sarah Anderson, Scott W. Gula, Kayleigh Hauri, Julius Eason, Megan Mulcahy, Scott Lee, James Michael Villegas, Patricia Shorter Sep 2020

Synergisms In Science: Climate Change And Integrated Pest Management Through The Lens Of Communication-2019 Student Debates, Jocelyn R. Holt, Lina Bernaola, Kadie E. Britt, Chirs Mccullough, Morgan Roth, Jennie Wagner, Max Ragozzino, Leslie Aviles, Zhilin Li, Forest Huval, Manoj Pandey, Benjamin W. Lee, Megan Asche, Abigail Hayes, Abigail Cohen, Adrian Marshall, Hannah E. Quellhorst, Rachel V. Wilkins, Valerie Nguyen, Jacqueline Maille, Rachel K. Skinner, John J. Ternest, Sarah Anderson, Scott W. Gula, Kayleigh Hauri, Julius Eason, Megan Mulcahy, Scott Lee, James Michael Villegas, Patricia Shorter

Faculty Publications

Every year, the Student Debates Subcommittee (SDS) of the Student Affairs Committee (SAC) for the annual Entomological Society of America (ESA) meeting organizes the Student Debates. This year, the SAC selected topics based on their synergistic effect or ability to ignite exponential positive change when addressed as a whole. For the 2019 Student Debates, the SAC SDS identified these topic areas for teams to debate and unbiased introduction speakers to address: 1) how to better communicate science to engage the public, particularly in the area of integrated pest management (IPM), 2) the influential impacts of climate change on agriculturally and …

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Multiple Decrement Life Tables Of Cephus Cinctus Norton (Hymenoptera: Cephidae) Across A Set Of Barley Cultivars: The Importance Of Plant Defense Versus Cannibalism, Buddhi B. Achhami, Robert K.D. Peterson, Jamie D. Sherman, Gadi V.P. Reddy, David K. Weaver Sep 2020

Multiple Decrement Life Tables Of Cephus Cinctus Norton (Hymenoptera: Cephidae) Across A Set Of Barley Cultivars: The Importance Of Plant Defense Versus Cannibalism, Buddhi B. Achhami, Robert K.D. Peterson, Jamie D. Sherman, Gadi V.P. Reddy, David K. Weaver

Faculty Publications

Accurately estimating cause-specific mortality for immature insect herbivores is usually difficult. The insects are exposed to abiotic and biotic mortality factors, causing cadavers to simply disappear before cause of mortality can be recorded. Also, insect herbivores are often highly mobile on hosts, making it difficult to follow patterns for individuals through time. In contrast, the wheat stem sawfly, Cephus cinctus Norton, spends its entire egg, larval, and pupal period inside a host stem. Therefore, with periodic sampling stage-specific causes of mortality can be ascertained. Consequently, we examined C. cinctus mortality in eight barley, Hordeum vulgare L., cultivars in two locations …

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Unpacking The Decline In Food Waste Measured In Chinese Households From 1991 To 2009, Danyi Qi, John W. Apolzan, Ran Li, Brian E. Roe Sep 2020

Unpacking The Decline In Food Waste Measured In Chinese Households From 1991 To 2009, Danyi Qi, John W. Apolzan, Ran Li, Brian E. Roe

Faculty Publications

© 2020 Elsevier B.V. Food waste reduction is an explicit goal for many countries, yet a paucity of high-quality primary measurements of food waste are available to inform policy. We analyze repeated physical measurements of discarded food from more than 37,000 households enrolled in the China Health and Nutrition Survey (CHNS) from 1991 to 2009 and describe relevant food waste patterns and trends within households over a period of dramatic change. Over a period in which average real household incomes tripled, food discarded per person declined by about 20% on a quantity basis and by about 40% on a Calorie …

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Electronic-Cigarette Vehicles And Flavoring Affect Lung Function And Immune Responses In A Murine Model, Brittany N. Szafran, Rakeysha Pinkston, Zakia Perveen, Matthew K. Ross, Timothy Morgan, Daniel B. Paulsen, Arthur L. Penn, Barbara L. F. Kaplan, Alexandra Noel Sep 2020

Electronic-Cigarette Vehicles And Flavoring Affect Lung Function And Immune Responses In A Murine Model, Brittany N. Szafran, Rakeysha Pinkston, Zakia Perveen, Matthew K. Ross, Timothy Morgan, Daniel B. Paulsen, Arthur L. Penn, Barbara L. F. Kaplan, Alexandra Noel

Faculty Publications

The use of electronic nicotine delivery systems (ENDS), also known as electronic-cigarettes (e-cigs), has raised serious public health concerns, especially in light of the 2019 outbreak of e-cig or vaping product use-associated acute lung injury (EVALI). While these cases have mostly been linked to ENDS that contain vitamin E acetate, there is limited research that has focused on the chronic pulmonary effects of the delivery vehicles (i.e., without nicotine and flavoring). Thus, we investigated lung function and immune responses in a mouse model following exposure to the nearly ubiquitous e-cig delivery vehicles, vegetable glycerin (VG) and propylene glycol (PG), used …

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Developing An Open-Source Lightweight Game Engine With Dnn Support, Haechan Park, Nakhoon Baek Sep 2020

Developing An Open-Source Lightweight Game Engine With Dnn Support, Haechan Park, Nakhoon Baek

Faculty Publications

With the growth of artificial intelligence and deep learning technology, we have many active research works to apply the related techniques in various fields. To test and apply the latest machine learning techniques in gaming, it will be very useful to have a light-weight game engine for quick prototyping. Our game engine is implemented in a cost-effective way, in comparison to well-known commercial proprietary game engines, by utilizing open source products. Due to its simple internal architecture, our game engine is especially beneficial for modifying and reviewing the new functions through quick and repetitive tests. In addition, the game engine …

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Twenty One Years After The Publication Of The Generalized Faustmann Formula, Sun Joseph Chang Sep 2020

Twenty One Years After The Publication Of The Generalized Faustmann Formula, Sun Joseph Chang

Faculty Publications

In this paper, I discuss developments of the generalized Faustmann formula since its publication in 1998. They included the formula for even-aged plantation management, uneven-aged management and forest valuation. These formula were followed by those for forest property taxation and valuation under taxation, the generalized van Kooten model, and the generalized Reed model as well as the uneven-aged management formula with carbon sequestration. The dynamic, incremental solution for the generalized Faustmann formula was then discussed. Pressler's indicator rate formula turned out to be equally applicable under the generalized Faustmann formula. Further, it enables the incorporation of risk preference to deal …

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Behavioral Strategies During Incubation Influence Nest And Female Survival Of Wild Turkeys, Ashley K. Lohr, James A. Martin, Gregory T. Wann, Bradley S. Cohen, Bret A. Collier, Michael J. Chamberlain Sep 2020

Behavioral Strategies During Incubation Influence Nest And Female Survival Of Wild Turkeys, Ashley K. Lohr, James A. Martin, Gregory T. Wann, Bradley S. Cohen, Bret A. Collier, Michael J. Chamberlain

Faculty Publications

Females must balance physiological and behavioral demands of producing offspring with associated expenditures, such as resource acquisition and predator avoidance. Nest success is an important parameter underlying avian population dynamics. Galliforms are particularly susceptible to low nest success due to exposure of ground nests to multiple predator guilds, lengthy incubation periods, and substantive reliance on crypsis for survival. Hence, it is plausible that nesting individuals prioritize productivity and survival differently, resulting in a gradient of reproductive strategies. Fine-scale movement patterns during incubation are not well documented in ground-nesting birds, and the influence of reproductive movements on survival is largely unknown. …

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Quantifying Species Traits Related To Oviposition Behavior And Offspring Survival In Two Important Disease Vectors, Donald A. Yee, William C. Glasgow, Nnaemeka F. Ezeakacha Sep 2020

Quantifying Species Traits Related To Oviposition Behavior And Offspring Survival In Two Important Disease Vectors, Donald A. Yee, William C. Glasgow, Nnaemeka F. Ezeakacha

Faculty Publications

Animals with complex life cycles have traits related to oviposition and juvenile survival that can respond to environmental factors in similar or dissimilar ways. We examined the preference-performance hypothesis (PPH), which states that females lacking parental care select juvenile habitats that maximize fitness, for two ubiquitous mosquito species, Aedes albopictus and Culex quinquefasciatus. Specifically, we examined if environmental factors known to affect larval abundance patterns in the field played a role in the PPH for these species. We first identified important environmental factors from a field survey that predicted larvae across different spatial scales. We then performed two experiments, …

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Tick-Borne Pathogens Shape The Native Microbiome Within Tick Vectors, Abdulsalam Adegoke, Deepak Kumar, Cailyn Bobo, Muhammad Imran Rashid, Aneela Zameer Durrani, Muhammad Sohail Sajid, Shahid Karim Sep 2020

Tick-Borne Pathogens Shape The Native Microbiome Within Tick Vectors, Abdulsalam Adegoke, Deepak Kumar, Cailyn Bobo, Muhammad Imran Rashid, Aneela Zameer Durrani, Muhammad Sohail Sajid, Shahid Karim

Faculty Publications

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Ticks are blood-feeding arthropods and transmit a variety of medically important viral, bacterial, protozoan pathogens to animals and humans. Ticks also harbor a diverse community of microbes linked to their biological processes, such as hematophagy, and hence affect vector competence. The interactions between bacterial and/or protozoan pathogens and the tick microbiome is a black-box, and therefore we tested the hypothesis that the presence of a protozoan or bacterial pathogen will alter the microbial composition within a tick. Hence, this study was designed to define the microbial composition of two tick species, …

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Masculinized Second-To-Fourth Digit Ratio (2d:4d Ratio) Is Associated With Lower Cortisol Response In Infant Female Rhesus Monkeys (Macaca Mulatta), Elizabeth K. Wood, Parker Jarman, Elysha Cash, Alexander Baxter, John P. Capitanio, James Dee Higley Sep 2020

Masculinized Second-To-Fourth Digit Ratio (2d:4d Ratio) Is Associated With Lower Cortisol Response In Infant Female Rhesus Monkeys (Macaca Mulatta), Elizabeth K. Wood, Parker Jarman, Elysha Cash, Alexander Baxter, John P. Capitanio, James Dee Higley

Faculty Publications

The second-to-fourth digit ratio (2D:4D ratio) is considered a postnatal proxy measure for the degree of prenatal androgen exposure (PAE), which is the primary factor responsible for masculinizing the brain of a developing fetus. Some studies suggest that the organizational effects of PAE may extend to the hypothalamic-pituitary-adrenal (HPA) axis response to stress. This study investigates the relationship between 2D:4D ratio and HPA axis functioning using a rhesus monkey (Macaca mulatta) model. Subjects were N = 268 (180 females, 88 males) rhesus monkey infants (3–4 months of age). Plasma cortisol concentrations were assayed from two blood samples obtained …

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Immune And Microrna Responses To Infection And Indole-3-Carbinol During Colitis, Rasha Raheem Alkarkoushi, Yvonne Hui, Abbas S. Tavakoli, Udai Singh, Prakash Nagarkatti, Mitzi Nagarkatti, Ioulia Chatzistamou, Marpe Bam, Traci L. Testerman Aug 2020

Immune And Microrna Responses To Infection And Indole-3-Carbinol During Colitis, Rasha Raheem Alkarkoushi, Yvonne Hui, Abbas S. Tavakoli, Udai Singh, Prakash Nagarkatti, Mitzi Nagarkatti, Ioulia Chatzistamou, Marpe Bam, Traci L. Testerman

Faculty Publications

BACKGROUND: Indole-3-carbinol (I3C) and other aryl hydrocarbon receptor agonists are known to modulate the immune system and ameliorate various inflammatory and autoimmune diseases in animal models, including colitis induced by dextran sulfate sodium (DSS). MicroRNAs (miRNAs) are also gaining traction as potential therapeutic agents or diagnostic elements. Enterohepatic (EHH) species are associated with an increased risk of inflammatory bowel disease, but little is known about how these species affect the immune system or response to treatment. AIM: To determine whether infection with an EHH species alters the response to I3C and how the immune and miRNA responses of an EHH …

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Counterion Condensation Or Lack Of Solvation? Understanding The Activity Of Ions In Thin Film Block Copolymer Electrolytes, Qi Lei, Ke Li, Deepra Bhattacharya, Jingya Xiao, Subarna Kole, Qingteng Zhang, Joseph Strzalka, Jimmy Lawrence, Revati Kumar, Christopher G. Arges Aug 2020

Counterion Condensation Or Lack Of Solvation? Understanding The Activity Of Ions In Thin Film Block Copolymer Electrolytes, Qi Lei, Ke Li, Deepra Bhattacharya, Jingya Xiao, Subarna Kole, Qingteng Zhang, Joseph Strzalka, Jimmy Lawrence, Revati Kumar, Christopher G. Arges

Faculty Publications

© The Royal Society of Chemistry. Polymer electrolytes are found at the heart of numerous electrochemical technologies involved in energy storage, conversion and separations. An important property of these materials is their partitioning behavior of ions in aqueous solutions and its effect on the activity of the ions within the polymer electrolyte. In this work, the fraction of condensed counterions (fc) were quantified in nanostructured block copolymer electrolyte (BCE) thin films with new and established experimental techniques. The transition between the osmotic-controlled regime and condensation-controlled regime in BCEs was identified using environmental GI-SAXS and solution uptake measurements via a quartz …

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Signals In The Soil: Subsurface Sensing, Abdul Salam, Usman Raza Aug 2020

Signals In The Soil: Subsurface Sensing, Abdul Salam, Usman Raza

Faculty Publications

In this chapter, novel subsurface soil sensing approaches are presented for monitoring and real-time decision support system applications. The methods, materials, and operational feasibility aspects of soil sensors are explored. The soil sensing techniques covered in this chapter include aerial sensing, in-situ, proximal sensing, and remote sensing. The underlying mechanism used for sensing is also examined as well. The sensor selection and calibration techniques are described in detail. The chapter concludes with discussion of soil sensing challenges.

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Decision Agriculture, Abdul Salam, Usman Raza Aug 2020

Decision Agriculture, Abdul Salam, Usman Raza

Faculty Publications

In this chapter, the latest developments in the field of decision agriculture are discussed. The practice of management zones in digital agriculture is described for efficient and smart faming. Accordingly, the methodology for delineating management zones is presented. Modeling of decision support systems is explained along with discussion of the issues and challenges in this area. Moreover, the precision agriculture technology is also considered. Moreover, the chapter surveys the state of the decision agriculture technologies in the countries such as Bulgaria, Denmark, France, Israel, Malaysia, Pakistan, United Kingdom, Ukraine, and Sweden. Finally, different field factors such as GPS accuracy and …

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Antarctica And The Southern Ocean, Jessica Blunden, Derek S. Arndt, E. Povl Abrahamsen, Sandra Barreira, Cecilia M. Bitz, Amy Butler, Kyle R. Clem, Steve Colwell, Lawrence Coy, Jos De Laat, Marcel D. Du Plessis, Ryan L. Fogt, Helen Amanda Fricker, John Fyfe, Alex S. Gardner, Sarah T. Gille, Tessa Gorte, L. Gregor, Will Hobbs, Bryan Johnson, Eric Keenan, Linda M. Keller, Natalya A. Kramarova, Matthew A. Lazzara, Jan T.M. Lenaerts, Jan L. Lieser, Hongxing Liu, Craig S. Long, Michelle Maclennan, Robert A. Massom, François Massonnet, Matthew R. Mazloff Aug 2020

Antarctica And The Southern Ocean, Jessica Blunden, Derek S. Arndt, E. Povl Abrahamsen, Sandra Barreira, Cecilia M. Bitz, Amy Butler, Kyle R. Clem, Steve Colwell, Lawrence Coy, Jos De Laat, Marcel D. Du Plessis, Ryan L. Fogt, Helen Amanda Fricker, John Fyfe, Alex S. Gardner, Sarah T. Gille, Tessa Gorte, L. Gregor, Will Hobbs, Bryan Johnson, Eric Keenan, Linda M. Keller, Natalya A. Kramarova, Matthew A. Lazzara, Jan T.M. Lenaerts, Jan L. Lieser, Hongxing Liu, Craig S. Long, Michelle Maclennan, Robert A. Massom, François Massonnet, Matthew R. Mazloff

Faculty Publications

No abstract provided.

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Characterizing The Epigenetic And Transcriptomic Responses To Perkinsus Marinus Infection In The Eastern Oyster Crassostrea Virginica, Kevin M. Johnson, K. A. Sirovy, Sandra M. Casas, Jerome F. La Peyre, Morgan W. Kelly Aug 2020

Characterizing The Epigenetic And Transcriptomic Responses To Perkinsus Marinus Infection In The Eastern Oyster Crassostrea Virginica, Kevin M. Johnson, K. A. Sirovy, Sandra M. Casas, Jerome F. La Peyre, Morgan W. Kelly

Faculty Publications

© Copyright © 2020 Johnson, Sirovy, Casas, La Peyre and Kelly. Eastern oysters in the northern Gulf of Mexico are routinely infected with the protistan parasite Perkinsus marinus, the cause of the disease commonly known as dermo. Recent experimental challenges among Atlantic coast populations have identified both resistant and susceptible genotypes using comparative transcriptomics. While controlled experimental challenges are essential first assessments, expanding this analysis to field reared individuals provides an opportunity to identify key genomic signatures of infection that appear both in the laboratory and in the field. In this study we combined reduced representation bisulfite sequencing with 3′ …

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Characterizing The Epigenetic And Transcriptomic Responses To Perkinsus Marinus Infection In The Eastern Oyster Crassostrea Virginica, Kevin M. Johnson, K. A. Sirovy, Sandra M. Casas, Jerome F. La Peyre, Morgan W. Kelly Aug 2020

Characterizing The Epigenetic And Transcriptomic Responses To Perkinsus Marinus Infection In The Eastern Oyster Crassostrea Virginica, Kevin M. Johnson, K. A. Sirovy, Sandra M. Casas, Jerome F. La Peyre, Morgan W. Kelly

Faculty Publications

© Copyright © 2020 Johnson, Sirovy, Casas, La Peyre and Kelly. Eastern oysters in the northern Gulf of Mexico are routinely infected with the protistan parasite Perkinsus marinus, the cause of the disease commonly known as dermo. Recent experimental challenges among Atlantic coast populations have identified both resistant and susceptible genotypes using comparative transcriptomics. While controlled experimental challenges are essential first assessments, expanding this analysis to field reared individuals provides an opportunity to identify key genomic signatures of infection that appear both in the laboratory and in the field. In this study we combined reduced representation bisulfite sequencing with 3′ …

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A Review Of Coal Heating Value Correlations With Application To Coal Char, Tar, And Other Fuels, Andrew P. Richards, Denver Haycock, Jacob F. Frandsen, Thomas H. Fletcher Aug 2020

A Review Of Coal Heating Value Correlations With Application To Coal Char, Tar, And Other Fuels, Andrew P. Richards, Denver Haycock, Jacob F. Frandsen, Thomas H. Fletcher

Faculty Publications

Accurately measuring a fuel’s heating value is one of the first steps in the classification of a new fuel. Heating values are widely used in coal combustion research and are becoming more useful in other fuel types as well. Many different empirical correlations to predict heating values based on primary organic (CHONS) elemental composition are found in the literature, many of which were originally created to predict heating values of parent coal, and some of which have been extended for biomass. However, no correlations exist for heating values of coal chars and tars. Thirteen literature heating value correlations (10 model …

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Tlr Antagonism By Sparstolonin B Alters Microbial Signature And Modulates Gastrointestinal And Neuronal Inflammation In Gulf War Illness Preclinical Model, Dipro Bose, Ayan Mondal, Punnag Saha, Diana Kimono, Sutapa Sarkar, Ratanesh K. Seth, Patricia Janulewicz, Kimberly Sullivan, Ronnie Horner, Nancy Klimas, Mitzi Nagarkatti, Prakash Nagarkatti, Saurabh Chatterjee Aug 2020

Tlr Antagonism By Sparstolonin B Alters Microbial Signature And Modulates Gastrointestinal And Neuronal Inflammation In Gulf War Illness Preclinical Model, Dipro Bose, Ayan Mondal, Punnag Saha, Diana Kimono, Sutapa Sarkar, Ratanesh K. Seth, Patricia Janulewicz, Kimberly Sullivan, Ronnie Horner, Nancy Klimas, Mitzi Nagarkatti, Prakash Nagarkatti, Saurabh Chatterjee

Faculty Publications

The 1991 Persian Gulf War veterans presented a myriad of symptoms that ranged from chronic pain, fatigue, gastrointestinal disturbances, and cognitive deficits. Currently, no therapeutic regimen exists to treat the plethora of chronic symptoms though newer pharmacological targets such as microbiome have been identified recently. Toll-like receptor 4 (TLR4) antagonism in systemic inflammatory diseases have been tried before with limited success, but strategies with broad-spectrum TLR4 antagonists and their ability to modulate the host-microbiome have been elusive. Using a mouse model of Gulf War Illness, we show that a nutraceutical, derived from a Chinese herb Sparstolonin B (SsnB) presented a …

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Exploiting Somatic Pirnas In Bemisia Tabaci Enables Novel Gene Silencing Through Rna Feeding, Mosharrof Mondal, Judith K. Brown, Alex Flynt Aug 2020

Exploiting Somatic Pirnas In Bemisia Tabaci Enables Novel Gene Silencing Through Rna Feeding, Mosharrof Mondal, Judith K. Brown, Alex Flynt

Faculty Publications

© This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). RNAi promises to reshape pest control by being nontoxic, biodegradable, and species specific. However, due to the plastic nature of RNAi, there is a significant variability in responses. In this study, we investigate small RNA pathways and processing of ingested RNAi trigger molecules in a hemipteran plant pest, the whitefly Bemisia tabaci. Unlike Drosophila, where the paradigm for insect RNAi technology was established, whitefly has abundant somatic piwi-associated RNAs (piRNAs). Long regarded as germline restricted, piRNAs are common in the …

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What Determines Parasite Species Richness Across Host Species?, Tad Dallas, Lauren A. Holian, Grant Foster Aug 2020

What Determines Parasite Species Richness Across Host Species?, Tad Dallas, Lauren A. Holian, Grant Foster

Faculty Publications

© 2020 British Ecological Society In Focus: Dáttilo, W., Barrozo-Chávez, N., Lira-Noriega, A., Guevara, R., Villalobos, F., Santiago-Alarcon, D., Neves, F. S., Izzo, T., & Ribeiro, S. P. (2020). Species-level drivers of mammalian ectoparasite faunas. Journal of Animal Ecology. https://doi.org/10.1111/1365-2656.13216. The question of what drives the number of parasite species able to infect a given host species is still a largely open question, despite decades of research. Dáttilo and colleagues examine the potential drivers of ectoparasite species across a large set of host species to explore the taxonomic and trait drivers of host-parasite interactions. Here, we contextualize their findings, explore …

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3-D Printed Customizable Vitrification Devices For Preservation Of Genetic Resources Of Aquatic Species, Connor J. Tiersch, Yue Liu, Terrence R. Tiersch, William T. Monroe Aug 2020

3-D Printed Customizable Vitrification Devices For Preservation Of Genetic Resources Of Aquatic Species, Connor J. Tiersch, Yue Liu, Terrence R. Tiersch, William T. Monroe

Faculty Publications

Sperm vitrification as an alternative approach to conventional cryopreservation (equilibrium freezing) allows quick and low-cost sample preservation and is suitable for small-bodied aquatic species with miniscule testis, fieldwork at remote locations, and small-scale freezing for research purposes. The goal of this present study was to develop operational prototypes of 3-dimensional (3-D) printed vitrification devices with innovative components that can provide comprehensive functionalities for practical repository development for aquatic species. The design featured an elongated loop to suspend a thin film of sperm sample in cryoprotectant, a retractable sleeve to protect the vitrified samples and allow permanent labeling, a handle to …

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Birds In Fragmented Amazonian Rainforest: Lessons From 40 Years At The Biological Dynamics Of Forest Fragments Project, Philip C. Stouffer Aug 2020

Birds In Fragmented Amazonian Rainforest: Lessons From 40 Years At The Biological Dynamics Of Forest Fragments Project, Philip C. Stouffer

Faculty Publications

For decades, ecologists have studied fundamental questions of how Amazonian biodiversity is maintained, and whether that diversity can persist following deforestation. The long history of avian research at the Biological Dynamics of Forest Fragments Project, near Manaus, Brazil, has helped advance this understanding in the context of a broader research program focused on rainforest fragments embedded in a dynamic matrix. By sampling birds beginning before fragments were isolated, in the late 1970s, and continuing the protocol to the present, our work has revealed community dynamics driven not just by area and isolation, but also by larger landscape patterns, particularly second …

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Temporal And Concentration Effects Of Methanol On Cryopreservation Of Zebrafish (Danio Rerio) Sperm, Huiping Yang, E. Hu, Terrence Tiersch, Carrie Carmichael, Jen Matthews, Zoltan M. Varga Aug 2020

Temporal And Concentration Effects Of Methanol On Cryopreservation Of Zebrafish (Danio Rerio) Sperm, Huiping Yang, E. Hu, Terrence Tiersch, Carrie Carmichael, Jen Matthews, Zoltan M. Varga

Faculty Publications

We evaluated the cryoprotective effects of methanol on zebrafish sperm at different concentrations, exposure times, and stages during cryopreservation. Samples were collected by crushing of dissected testes or abdominal stripping. After exposure to 0%, 2%, 5%, 8%, and 10% methanol for 0-11 min, fresh sperm (1 × 106 cells/mL) did not show changes in plasma membrane integrity (measured by flow cytometer), but cell size changes (light scatter) were observed after exposure to 8% or 10%. After exposure for 0-60 min, fresh sperm (1 × 108 cells/mL) did not show significant changes in survival or membrane integrity. Sperm cryopreserved in 5%, …

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Using Public Databases For Genomic Prediction Of Tropical Maize Lines, Pedro Patric Pinho Morais, Deniz Akdemir, Luciano Rogério Braatz De Andrade, Jean Luc Jannink, Roberto Fritsche-Neto, Aluízio Borém, Filipe Couto Alves, Danilo Hottis Lyra, Ítalo Stefanine Correia Granato Aug 2020

Using Public Databases For Genomic Prediction Of Tropical Maize Lines, Pedro Patric Pinho Morais, Deniz Akdemir, Luciano Rogério Braatz De Andrade, Jean Luc Jannink, Roberto Fritsche-Neto, Aluízio Borém, Filipe Couto Alves, Danilo Hottis Lyra, Ítalo Stefanine Correia Granato

Faculty Publications

In this paper, the aims were (a) to test the usefulness of using genomic and phenotypic information from public databases (open access) to predict genetic values for tropical maize inbred lines regarding plant and ear height; (b) to identify how the population structure, the use of optimized training sets (OTSs) and the amount of information originating from public databases affect the predictive ability. Thus, 29 training sets (TSs) were defined considering three diversity panels: the University of São Paulo (USP—validation set (VS)) and the ASSO and USDA North Central Regional Plant Introduction Station (NCRPIS) (external public panels—predictors), which were divided …

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The "Challenge" Of Depletion: Why The Oyster Fishery Is Not Self-Regulating, Eric N. Powell, John M. Klinck, Leanne M. Poussard Aug 2020

The "Challenge" Of Depletion: Why The Oyster Fishery Is Not Self-Regulating, Eric N. Powell, John M. Klinck, Leanne M. Poussard

Faculty Publications

© 2020 National Shellfisheries Association. All rights reserved. The possibility that the economics of the oyster fishery impose a self-limitation on overharvesting has been proffered on occasion. The inefficiency of harvesting by the fishery has been evaluated and estimates of the exploitation rate permissible under conditions of maximum sustainable yield have been obtained in previous studies. The question becomes to what extent does the inefficiency of harvest interact with the economics of the fishery to compromise ready detection of overfishing? This study explores the possibility that the constraint of economics on the fishery occurs at oyster exploitation rates that are …

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Democracy, Deference, And Compromise: Understanding And Reforming Campaign Finance Jurisprudence, Scott P. Bloomberg Aug 2020

Democracy, Deference, And Compromise: Understanding And Reforming Campaign Finance Jurisprudence, Scott P. Bloomberg

Faculty Publications

In Citizens United, the Supreme Court interpreted the government’s interest in preventing corruption as being limited to preventing quid pro quo—cash-for-votes—corruption. This narrow interpretation drastically circumscribed legislatures’ abilities to regulate the financing of elections, in turn prompting scholars to propose a number of reforms for broadening the government interest in campaign finance cases. These reforms include urging the Court to recognize a new government interest such as political equality, to adopt a broader understanding of corruption, and to be more deferential to legislatures in defining corruption. Building upon that body of scholarship, this Article begins with a descriptive account of …

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The Invisible Message, Andrew S. Gibbons Iii, Elizabeth Boling Aug 2020

The Invisible Message, Andrew S. Gibbons Iii, Elizabeth Boling

Faculty Publications

The number and variety of messages conveyed by an instructional experience is astonishing, but most designers are unaware of their number, subtlety, and impact. Many of those messages they would not choose to send if they recognized their existence in practice. The design of invisible and abstract message structures receives less attention from designers today than those parts of the design given to more vivid, colorful, and showy surface structures. Invisible message structures work behind the scenes to produce the smooth surface performances in front of the curtain; they are seldom seen directly, but their power is indisputable. The purpose …

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Exploring The Louisiana Purchase And Its Borderlands: The Lewis And Clark, Hunter And Dunbar, Zebulon Pike, And Freeman And Custis Expeditions In Perspective [Part 1], Jay H. Buckley Aug 2020

Exploring The Louisiana Purchase And Its Borderlands: The Lewis And Clark, Hunter And Dunbar, Zebulon Pike, And Freeman And Custis Expeditions In Perspective [Part 1], Jay H. Buckley

Faculty Publications

When Americans contemplate the exploration of the Louisiana Purchase, the names Meriwether Lewis and William Clark quickly come to mind. Their historic journey was well conceived, well executed, and well documented. It was one of the first and longest U.S. government-backed explorations of the American West. The name Zebulon Pike, for some, conjures up notions of a lost explorer who was arrested by the Spanish as an alleged American spy. Fewer still have heard of the names William Dunbar, George Hunter, Thomas Freeman, and Peter Custis. Even those who have heard of these men may be hard-pressed to identify any …

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