Digital Commons Network^™

Magnetic Nanosensors For Multiplexed Bacterial Pathogenesis Identification, Charalambos Kaittanis

Electronic Theses and Dissertations

Developing diagnostic modalities that utilize nanomaterials and miniaturized detectors can have an impact in point-of-care diagnostics. Diagnostic systems that (i) are sensitive, robust, and portable, (ii) allow detection in clinical samples, (iii) require minimal sample preparation yielding results quickly, and (iv) can simultaneously quantify multiple targets, would have a great potential in biomedical research and public healthcare. Bacterial infections still cause pathogenesis throughout the world (Chapter I). The emergence of multi-drug resistant strains, the potential appearance of bacterial pandemics, the increased occurrence of bacterial nosocomial infections, the wide-scale food poisoning incidents and the use of bacteria in biowarfare highlight the …

An Assessment Of Sea Turtle Nesting Behavior In Relation To Hurricane- And Restoration-Induced Beach Morphodynamics, Tonya Michele Long

Electronic Theses and Dissertations

Coastal habitats are highly dynamic and vulnerable to landscape-level disturbances such as storms and restoration projects. Along the east coast of Florida these areas are particularly valuable as they provide significant nesting habitat for two sea turtle species, the threatened loggerhead (Caretta caretta) and the endangered green turtle (Chelonia mydas). This coast was heavily impacted by three major hurricanes in 2004 and in some areas by large restoration projects in 2005. Recent remote sensing methods allow for broad evaluation of the shoreline and thus the ability to assess sea turtle nesting habitat at a landscape scale. I collected nesting data …

Sexual Dimorphism Of The Posterior Pelvis Of The Robert J. Terry Anatomical Collection And The William M. Bass Donated Skeletal Collection, Lauren M. Novak

Electronic Theses and Dissertations

Studies of sexual dimorphism of the sacrum have generally been conducted as part of broader population research or on living persons and cadavers, making the anthropological literature sparse. The greater sciatic notch and the preauricular sulcus of the ilium have both been found to show sexual dimorphism, although studies of these traits often have ambiguous definitions of characteristics and lack the standardization of measurements. This research was designed to reexamine and test the accuracy of standard scoring systems and measurements of the posterior pelvis used to determine sex and to establish new formulae combining traits and measurements to accurately determine …

Geographic Variation In Post-Mating Immune Gene Expression In Drosophila Melanogaster, Cheryl Ann Pinzone

Electronic Theses and Dissertations

An organism's immune response may vary due to pathogen pressure in its environment, as well as due to interactions with other organisms. These factors, along with geographic rules (i.e. Gloger's rule) may influence the geographic distribution of the immune response within populations of a species. Here we use real-time quantitative PCR to measure the immune gene expression in six populations collected along the eastern U.S. of Drosophila melanogaster after mating. Antimicrobial genes did not show significant differences in expression due to location, whereas we did observe differences in anti-fungal and pro-phenoloxidase (anti-macromolecule) related genes. These differences in anti-macromolecule resistance are …

Effects Of A Treatment Using Computer Generation Of Isometric And Orthographic Projections On Middle School Students' Spatial Ability, Adam Michael Traas

Electronic Theses and Dissertations

The primary focus of this study examines the effectiveness of the CRIOSAT (Computerized Rotational Isometric and Orthographic Spatial) spatial ability treatment on a random sample of middle school students’ (n=137) spatial ability as measured by the Purdue Spatial Visualization Test: Rotations Test (PSVT-ROT) (Guay, 1977). The secondary focus of this study investigates the relationships between mathematical achievement, problem solving preferences, and spatial ability. The secondary focus was tested on a subsample (n=41), with the problem solving preferences measured via the Mathematical Processing Instrument (MPI) (Suwarsono, 1982). Findings indicated no significant gains in spatial ability scores after students’ use of the …

Electroconvulsive Shock Ameliorates Disease Processes And Extends Survival In Huntington Mutant Mice, Akanksha Baharani

Electronic Theses and Dissertations

Huntington's disease (HD) is a devastating autosomal dominantly inherited neurological disorder caused by an abnormal expansion of CAG trinucleotide repeats in the gene coding for the Nterminal region of the huntingtin (Htt) protein, which leads to the formation of a polyglutamine stretch. The greater the CAG repeats, the earlier the onset of the disease. The polyglutamine stretch destabilizes the Htt protein leading to misfolding, abnormal processing, aggregation, and inclusion formation. Mutant Htt protein is believed to damage and kill neurons in the striatum by a mechanism involving increased oxidative and metabolic stress, and impaired adaptive cellular stress responses. A large …

Acetazolamide-Induced Decrease Of Apical Fluid Flow In Choroid Plexus Is Independent Of The Concomitant Changes In Aquaporin-1 Expression, Pouya Alexander Ameli

Electronic Theses and Dissertations

Acetazolamide (AZA), the only drug approved for treatment of hydrocephalus, is effective in only 25-30% of patients while its effect on fluid flow in the choroid plexus (CP) is unknown. The drug reversibly inhibits Aquaporin 4 (AQP4), the most highly expressed „water pore‟ in the brain, and it is postulated that it reduces cerebrospinal fluid (CSF) production by modulating AQP1 (mostly found in the apical membrane of the CP). In this study, we sought to elucidate the effect of AZA on AQP1 and fluid flow in CP. Primary CP culture from p10 Sprague-Dawley rats and TRCSF-B cell line were grown …

Design, Synthesis And Characterization Of New Two-Photon Absorbing (2pa) Fluorescent Dyes And Bioconjugates, And Their Applications In Bioimaging, Carolina D. Andrade

Electronic Theses and Dissertations

The development of new multiphoton absorbing materials has attracted the attention of researchers for the last two decades. The advantages that multiphoton absorbing materials offer, versus their one-photon absorbing counterparts, rely on the nature of the nonlinearity of the absorption process, where two photons are absorbed simultaneously offering increased 3D resolution, deeper penetration, and less photobleaching and photodamage as a result of a more confined excitation. The applications of efficient two-photon absorbing materials have been extensively expanding into the fields of photodynamic therapy, microscopy, and optical data storage. One of the fields where an increased interest in multiphoton absorbing materials …

Estimating Diet And Food Selectivity Of The Lower Keys Marsh Rabbit Using Stable Isotope Analysis, Matthew James Gordon

Electronic Theses and Dissertations

Understanding the effect of food abundance on feeding behavior can benefit conservation efforts in many ways, such as to determine whether impacted environments need food supplementation, whether different locations of threatened species contain different food abundances, or whether reintroduction sites are missing key components of a species’ diet. I studied the relationship between feeding behavior and food abundance in the Lower Keys marsh rabbit (Sylvilagus palustris hefneri), an endangered subspecies endemic to the lower Florida Keys. Specifically, my study set out to measure the relative abundance of the primary plants within the natural habitat of the Lower Keys marsh rabbit …

The Communication Implications And Related Experiences Associated With Transracially Adopting A Child From Vietnam, Lan Marie Malin

Electronic Theses and Dissertations

This study investigated the communication experiences of adoptive parents of children transracially adopted from Vietnam. Though adoption has been extensively studied in communication research, transracial adoptions involving children from Vietnam has not. Thus, this study examined adoptive parent communication experiences using dialectic theory and relational dialectics. By examining adoptive parents’ communication with their adopted child and others, we can determine tensions that occur in different communication experiences. Data were collected through eight qualitative in-depth interviews conducted with adoptive parents of children from Vietnam. Openness with both strangers and the adopted child(ren) and preservation of key aspects of the adopted children’s …

Transplantation Of Ips Cells Reduces Apoptosis And Fibrosis And Improves Cardiac Function In Streptozotocin-Induced Diabetic Rats, Sarah Elizabeth Neel

Electronic Theses and Dissertations

Background: Streptozotocin (STZ) induced diabetes leads to various complications including cardiomyopathy. Recent data suggests transplanted bone marrow stem cells improve cardiac function in diabetic cardiomyopathy. However, whether modified ES, iPS cells, or factors released from these cells can inhibit apoptosis and fibrosis remains completely unknown. The present study was designed to determine the effects of transplanted ES cells overexpressing pancreatic transcription factor 1 a (Ptf1a), a propancreatic endodermal transcription factor, iPS cells, or their respective conditioned media (CM) on diabetic cardiomyopathy. Methods: Experimental diabetes was induced in male Sprague Dawley rats (8-10 weeks old) by intraperitoneal STZ injections (65 mg/kg …

Initial-Value Technique For Singularly Perturbed Two Point Boundary Value Problems Via Cubic Spline, Luis G. Negron

Electronic Theses and Dissertations

A recent method for solving singular perturbation problems is examined. It is designed for the applied mathematician or engineer who needs a convenient, useful tool that requires little preparation and can be readily implemented using little more than an industry-standard software package for spreadsheets. In this paper, we shall examine singularly perturbed two point boundary value problems with the boundary layer at one end point. An initial-value technique is used for its solution by replacing the problem with an asymptotically equivalent first order problem, which is, in turn, solved as an initial value problem by using cubic splines. Numerical examples …

The Role Of The Feral Pig (Sus Scrofa) As A Disturbance Agent And Seed Disperser In Central Florida's Natural Lands, Camille Rose Brescacin

Electronic Theses and Dissertations

Feral pigs (Sus scrofa) are considered to be among the world’s worst invasive species due to their successful invasion and ecological and economic impact to native and agricultural plants and animals around the world. Feral pigs are significant disturbance agents that destroy plant communities, change soil characteristics, alter nutrient cycling, and create open sites for colonization of both native and non-native plant species through their foraging behavior called rooting. In contrast to native animal disturbances, rooting is a striking feature in the landscape that varies in space, seasonal timing, frequency (number of times rooted), and intensity (depth of rooting). During …

A Self-Organizing Hybrid Sensor System With Distributed Data Fusion For Intruder Tracking And Surveillance, Ravishankar Palaniappan

Electronic Theses and Dissertations

A wireless sensor network is a network of distributed nodes each equipped with its own sensors, computational resources and transceivers. These sensors are designed to be able to sense specific phenomenon over a large geographic area and communicate this information to the user. Most sensor networks are designed to be stand-alone systems that can operate without user intervention for long periods of time. While the use of wireless sensor networks have been demonstrated in various military and commercial applications, their full potential has not been realized primarily due to the lack of efficient methods to self organize and cover the …

7 An Interactive Installation: Explorations In The Digital, The Spiritual, And The Uncanny, Bradley Paul Lewter

Electronic Theses and Dissertations

This thesis explores the application of digital technologies in the creation of visionary or transformative artwork. The installation emphasizes number, color, symmetry, and the human form to create symbolic compositions patterned after ancient archetypes. Background research was done to inform the work through studies of the principles of visionary and transformative artwork as practiced by Ernst Fuchs, De Es Schwertberger, and Alex Grey. Connections between art and spirituality as explained by Kandinsky were studied to augment these principles. The sequence of artwork within the installation is comprised of both digital paintings and interactive triptych panels. To convey a sense of …

Evelyn Offscreen: An Application Of Interactive Performance Methodsin Alternate Reality Gaming, Dawn Borglund

Electronic Theses and Dissertations

For my thesis project for the Masters of Fine Art in Film and Digital Media, I designed and produced Evelyn Offscreen, an alternate reality game (ARG) that was facilitated by interactive performance. The goal was to create an interactive experience that allowed several players to collaboratively create story across numerous media within the field of alternate reality gaming. The approach used in Evelyn Offscreen was intended to provide a degree of creative freedom to the players that has not been demonstrated in other ARG experiences and to use digital media to capture information about the relative effectiveness of the different …

Flirting As A Liminal Experience: A Dialogue Between A Philosophical-Phenomenological Understanding Of Liminality And An Empirical-Existential Study Of Flirting, Rune Lassen Mølbak

Electronic Theses and Dissertations

In this dissertation I bring out concealed aspects of the lived experience of flirting by studying it neither as a subjective act nor as an objective behavior, but as a liminal event. Liminality is an anthropological concept that designates a realm of experience that falls outside identity and mutually exclusive binaries. I argue that flirting is a liminal experience in so far as it is an experience that both constitutes me through the other and is constituted by me as a way of relating to the other. What is primary is thus not self or other, but the relation or …

Women Leaders Resolving Conflict In Higher Education: A Feminist Epistemological Perspective, Maureen C. Silva

Electronic Theses and Dissertations

In an American postsecondary context, conflict is inherent (Gianneschi & Yanagiura, 2006; Valian, 1999). Successful navigation of conflict in the academy is vital for those who aspire to leadership positions (Nadler & Nadler, 1987; Walters, Stuhlmacher, & Meyer, 1998). Presently, however, women face significant barriers to achieving success in higher education administration, including gender expectations for conflict resolution behavior (Bartunek, 1992; Bowles, Babcock, & McGinn, 2005; Gayle, Preiss, & Allen, 2002).

While a considerable body of literature exists for understanding gender negotiation, it remains rooted in a masculine paradigm (Kolb & Putnam, 2006; Shuter & Turner, 1997), and, as such, …

Dopamine D2 Receptor Priming Enhances Dopaminergic Response To Amphetamine In The Nucleus Accumbens: Role Of The D1 And D2 Receptors., Kimberly Norris Huggins

Electronic Theses and Dissertations

In past work, we have shown neonatal quinpirole (dopamine D₂/D₃ agonist) treatment produces a significant increase in dopamine D₂ receptor sensitivity, a phenomenon known as D₂ receptor priming. Dopamine D₂ receptor priming is common in psychosis. Male and female rats were administered quinpirole (1mg/kg) or saline from postnatal days 1-11 and raised to adulthood (P60). As adults, rats were administered d-amphetamine sulfate (1mg/kg) or saline every other day for 14 days. Approximately 10 min before each amphetamine or saline injection, animals were administered the D1 antagonist SCH 23390 (0.1 mg/kg), the D2 antagonist eticlopride …

Comparison Of Ammonia And Particulate Matter Air Sample Concentrations At Task-Locations Within Swine Confinement Buildings., Earl Dan Bembry

Electronic Theses and Dissertations

Introduction: A task-associated analysis of ammonia and particulate matter concentrations was conducted on swine farms in breeding and gestation barns. The purpose of this study was to determine if specific tasks performed by workers increase concentrations of ammonia and particulate matter exposure.

Methods: An exploratory, descriptive design was used to determine if swine confinement workers have increased ammonia and particulate matter exposure when performing assigned tasks in breeding and gestation barns. A convenience sample of 8 workers volunteered from among all breeding and gestation workers (n=24). Data collection occurred in the morning at each of the 8 farms …

A Qualitative Case Study Of Natural Storytelling Strategies Used At The Nantahala School., Nancy J. Reeder

Electronic Theses and Dissertations

This qualitative case study identifies what 8 teachers naturally do with storytelling at a K-12 school in western North Carolina. Through observations, interviews, field notes, document reviews, and artifact reviews, I used the constant comparison analysis method to discover themes from their formal and informal storytelling strategies. From these themes, I created a framework of 7 suggestions to enhance storytelling awareness. They are: sharing personal stories, developing voice control and body expression, connecting story to school culture, modeling well-developed stories with beginnings, middles, and ends, introducing stories with a pause, expressing the word "imagine" often during instruction, and finally, making …

Genetic Structure Of Yonahlossee Salamander Populations., Joshua Andrew Rudd

Electronic Theses and Dissertations

Plethodon yonahlossee is the largest eastern Plethodontid salamander. It has been classified as a species of greatest conservation need by the Tennessee Wildlife Resources Agency (TWRA). Found only in mountainous areas along the borders of Tennessee, North Carolina, and Virginia, populations of the yonahlossee are considered to be rare and local throughout their range. Genetic differentiation among populations of any species is usually attributable to long-standing, extrinsic barriers to gene flow. Because of their disjunct population structure and some observed morphological variation, genetic differentiation among yonahlossee populations is expected. A genetic structure study of yonahlossee was conducted to identify any …

Vitis Seeds (Vitaceae) From The Late Neogene Gray Fossil Site, Northeastern Tennessee, Usa., Fade Gong

Electronic Theses and Dissertations

This study focuses on the morphometric and systematic studies of fossil vitaceous seeds recently recovered from the Gray Fossil Site (7-4.5 Ma, latest Miocene-earliest Pliocene) northeastern Tennessee. Morphologically, all fossil seeds correspond to the extant subgenus Vitis (genus Vitis) of the Vitaceae based on the smooth dorsal surface with a centrally positioned chalaza connected with a conspicuous chalaza-apex groove and short linear ventral infolds that are slightly diverged apically. A multivariate analysis based on 11 measured characters from 76 complete seeds identified three types of seeds, each representing a distinct morphotaxon. Based on comparison with modern and fossil vitaceous …

Predicting Flavonoid Ugt Regioselectivity With Graphical Residue Models And Machine Learning., Arthur Rhydon Jackson

Electronic Theses and Dissertations

Machine learning is applied to a challenging and biologically significant protein classification problem: the prediction of flavonoid UGT acceptor regioselectivity from primary protein sequence. Novel indices characterizing graphical models of protein residues are introduced. The indices are compared with existing amino acid indices and found to cluster residues appropriately. A variety of models employing the indices are then investigated by examining their performance when analyzed using nearest neighbor, support vector machine, and Bayesian neural network classifiers. Improvements over nearest neighbor classifications relying on standard alignment similarity scores are reported.

Initial Development Of The Sex Offender Attitude Scale., Brandon Coy Bogle

Electronic Theses and Dissertations

As long as the public maintains staunch and unwarranted negative attitudes toward sex offenders, little hope remains for released sex offenders to successfully reintegrate into society. Therefore, it is in the interest of society that we understand the nature of views of sex offenders so that attempts can be made to promote maximal reintegration and preventing recidivism. To date no valid psychometric instrument exists that measures attitudes toward sex offenders. Therefore, the purpose of this project is to develop such an instrument that will accurately measure stigma associated with sex offenders. Unrotated Prinicipal Components Factor Analysis revealed a single interpretable …

Positive Psychological And Religious Characteristics As Moderators Of Negative Life Events And Depressive Symptoms: A Multiethnic Comparison., Preston Lee Visser

Electronic Theses and Dissertations

Hope, optimism, and several markers of religiosity and spirituality were examined as potential moderators of the association between negative life events and depressive symptoms in a secondary data analysis of an ethnically diverse sample. Participants (267 female, 119 male) were college students enrolled at an urban Northeastern university. It was hypothesized that negative life events would be associated with increased depressive symptoms and that higher levels of hope, optimism, and religious and spiritual variables would attenuate this relationship. Ethnically-stratified moderation analyses were conducted to assess for differences in moderation between Blacks, Hispanics, Whites, and Asians. Hypotheses were generally supported, with …

The Role Of Self-Efficacy, Family Support, Family Affection, And Family Conflict On Adolescent Academic Performance., Christine L. Pearson

Electronic Theses and Dissertations

The Department of Education funded this study as a part of a larger longitudinal study to examine the relationship between the role of family environment factors and academic performance among adolescents. The participants included 685 middle school adolescents from rural and semirural public schools. Family environment factors were gathered using the Family Environment Scale (Moos & Moos, 1981) and included family conflict, family support, and showing affection. Standardized test scores across 4 domains and final course grades across 4 domains were collapsed and used as measures of academic performance. The moderating relationship between support, conflict, and academic performance was examined. …

Evaluating The School Performance Of Elementary And Middle School Children Of Incarcerated Parents., Melissa F. Neal

Electronic Theses and Dissertations

Children of incarcerated parents are at significantly increased risk of negative long-term outcomes. With about 1% of the adult population incarcerated, the United States has millions of children at risk for these negative outcomes. Research on this population is increasing; however, it is still unclear whether children of incarcerated parents are at an increased risk for poor school performance as a specific result of parental incarceration above that associated with their social and economic status. Because parental incarceration may result in a variety of outcomes that can negatively impact school performance including school mobility, prolonged exposure to stress, and insufficient …

Reproductive Success Of American Kestrels (Falco Sparverius) Nesting In Boxes Along An Interstate In Northeastern Tennessee., Jennifer Robertson Powers

Electronic Theses and Dissertations

Nest box programs provide supplemental nest sites for American Kestrels, Falco sparverius. When the availability of nest sites is a limiting factor, the addition of nest boxes can increase local breeding populations. These programs also facilitate the collection of data on breeding kestrels.

This study focuses on an American Kestrel (Falco sparverius) nest box trail along Interstate 26 in northeastern Tennessee during the breeding seasons of 1998, 1999, 2000, 2003, 2004, 2006, and 2009. Productivity measures and reproductive success of nesting birds are provided and compared to other programs. The data are analyzed across years and by …

Investigating Nectar Rhythms In Squash (Cucurbita Pepo): Effects On Honey Bee (Apis Mellifera) Foraging Behavior., Samuel David Boyd

Electronic Theses and Dissertations

Experiments were performed to investigate the influence of water availability on the diel patterns of nectar secretion (volume, concentration, sugar production) in male squash flowers as well as to discover what physical component of nectar honey bees use to trigger their time-memory. Squash plants were grown in the greenhouse and in the field under both constant and variable watering regimes. Throughout anthesis, nectar volume and sugar concentration were recorded. In the field, the temporal distribution of arrivals to squash was observed with and without blossoms present. In the greenhouse and in the field, squash flowers exhibit a consistent diel pattern …