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Place, Race, And Religion In The Local Lesbian, Gay, Bisexual, Transgender Community And Movement Of Memphis, Tennessee, Amy Catherine Ulmer

Electronic Theses and Dissertations

This study observes the role of place in the local LGBT community and movement of Memphis, Tennessee. I gathered information from fifteen interviews, including LGBT Memphians, activists, preachers, and public figures to show what aspects of place have been the most significant in shaping the nature of the local movement, which has been growing since the early 2000s. I suggest that the conservatism, race, and religiosity of Memphis have played the most significant role. The first chapter demonstrates how LGBTs operate within a city that maintains pockets of openness, but remains largely LGBT-unfriendly according to interviewees. The second chapter observes …

Fret Studies Of Dna Binding By Lambda Cro Variants, Lei Wang

Electronic Theses and Dissertations

Proteins bind to DNA to form switches and circuits that control the flow of genetic information from DNA sequence to the physical components of cells and organisms. The dynamic performance of genetic switches depends on the coupling of the comprocesses of transcription and translation to protein-specific folding, assembly and binding reactions. For many model circuits protein dimerization and DNA binding are fast relative to the time scale of transcription and translation and thus amenable to modeling by statistical thermodynamics. In these cases, the distribution of proteins between monomers and dimers and between nonspecific and specific DNA complexes can be simply …

Isolation, Structure Elucidation And Biological Evaluation Of Natural Products From Plants And Fungi, Xiaoning Wang

Electronic Theses and Dissertations

Natural products have provided considerable value for drug discovery over the past half-century. Nearly four fifth of drugs were natural products or inspired by natural compounds. Bioactive secondary metabolites isolated from natural source, either fungal or terrestrial, are relatively safer for human health and the environment and can serve as drug or as drug leads for the development of synthetic or semi-synthetic analogs. Natural products have also been successfully used in agriculture as plant growth regulators, signal transductions, fungicides, herbicides and tranquilizers. In our continued efforts to identify bioactive compounds, seventy-seven plant and fungal extracts were evaluated using a direct-bioautography …

Antileishmania Compounds From An Unidentified Endophytic Fungus And Biotransformation Of 5,7,2`,4`,5`-Pentahydroxyflavonol, Ahmed Idris

Electronic Theses and Dissertations

Leishmaniasis is a parasitic disease remaining a major public health problem. It causes illness and death especially in developing countries. Comchemotherapeutic agents currently used are often inadequate, requiring long courses of parenteral administration, having toxic side effects or becoming less effective due to the emergence of resistant strains. Thus, there is an urgent need for new, effective, and inexpensive drugs. In the fight against leishmaniasis, natural products are important sources of novel therapeutic agents. The goal of our research is to study secondary metabolites from endophytic fungi as a source of effective antileishmanial agents. Many crude extracts from endophytic fungi …

Discovery Of Novel Glycogen Synthase Kinase-3beta Inhibitors: Molecular Modeling, Virtual Screening, And Biological Evaluation, Gang Fu

Electronic Theses and Dissertations

Glycogen synthase kinase-3 (GSK-3) is a multifunctional serine/threonine protein kinase which is engaged in a variety of signaling pathways, regulating a wide range of cellular processes. Due to its distinct regulation mechanism and unique substrate specificity in the molecular pathogenesis of human diseases, GSK-3 is one of the most attractive therapeutic targets for the unmet treatment of pathologies, including type-II diabetes, cancers, inflammation, and neurodegenerative disease. Recent advances in drug discovery targeting GSK-3 involved extensive computational modeling techniques. Both ligand/structure-based approaches have been well explored to design ATP-competitive inhibitors. Molecular modeling plus dynamics simulations can provide insight into the protein-substrate …

Survey Of The Native And Nonnative Vascular Plant Species Of Three Islands In Lake Winnipesaukee, New Hampshire, Mark Gardner Winkler

Electronic Theses and Dissertations

This study examined the long-term patterns in vascular plant composition on three islands in Lake Winnipesaukee, NH. It also tested the role of island biogeography in ecology as it analyzed the effectiveness of a land use plan implemented on the islands. Samplings were made on the islands in the summer of 2011 and were compared to earlier samplings in 1978, 1991, and 2001. The flora was observed and measured in 25 permanent plots that were established on the three islands in 1978. The understory flora was measured by presence and percent cover and the overstory was measured by frequency and …

Geographic And Host-Microbe Symbiotic Influence On Secondary Metabolism, Samuel Hamed Abbas

Electronic Theses and Dissertations

This thesis is composed of studies that regard the advancement to the Arctic region and the importance of host-microbe interactions in natural product discovery. Bioactive metabolites have been reported from a myriad of marine and terrestrial organisms around the world including plants, insects, sponges, tunicates, bacteria, and fungi among others. Many macroorganisms, of which the metabolites are found, depend on the symbiotic relationship of microorganisms for metabolite production. The scope of this work is to investigate secondary metabolism of both marine and terrestrial organisms from different areas of the world as well as search for the importance of host-microbe symbiosis …

Restricting Supervised Learning: Feature Selection And Feature Space Partition, Xiaofei Nan

Electronic Theses and Dissertations

Many supervised learning problems are considered difficult to solve either because of the redundant features or because of the structural complexity of the generative function. Redundant features increase the learning noise and therefore decrease the prediction performance. Additionally, a number of problems in various applications such as bioinformatics or image processing, whose data are sampled in a high dimensional space, suffer the curse of dimensionality, and there are not enough observations to obtain good estimates. Therefore, it is necessary to reduce such features under consideration. Another issue of supervised learning is caused by the complexity of an unknown generative model. …

Bacterial And Sediment Transport In An Artificial Sand Bed Stream During Unsteady Flow, Mary Alexandra Mccaskill

Electronic Theses and Dissertations

Storms cause a substantial increase in the fecal indicator bacteria (FIB) concentrations in stream water. Causes for this concentration increase include FIB-laden stormwater runoff and the release of bacteria from stream-bottom sediments. Studies were carried out to quantify this second cause of water quality impairment. Coliform bacteria are a group of FIB, indicate the presence of pathogenic microorganisms, and have been shown to be transported in streams far from their source areas, especially during storms. FIB transport is dependent on several factors including water velocity, size and transport rates of bed sediments, and the FIB concentration in bed sediment. The …

The Queen Of The Household: Mothers, Other Mothers, And Female Genealogy On The Plantation In Postslavery Women's Fiction, Correna Catlett Merricks

Electronic Theses and Dissertations

In many ways, the plantation defined the U.S. South because it was the primary site of production, and therefore income, for prominent southerners. In addition to being a site of production, the plantation created a complex series of connected relationships that was imagined by the plantocracy to be a large family unit. It functioned according to a specific hierarchical model that was primarily based on a patriarchal understanding of genealogy. Yet Kate Chopin's "Désirée's Baby" and "La Belle Zoraïde," Pauline Hopkins's Contending Forces, Zora Neale Hurston's Their Eyes Were Watching God, Julia Peterkin's Scarlet Sister Mary, Eudora Welty's Delta Wedding, …

Development And Comparative Predictive Validity Of An Outpatient Medication Exposure Measure For Risk Adjustment Using Retrospective Claims Data, Kyle Dennis Null

Electronic Theses and Dissertations

The purpose of this study was to develop and test a measure of outpatient prescription utilization (medication exposure measure, or MEM) that may be coupled with the CMS-HCC and CMS-RxHCC methodologies to improve risk-adjusted payments to Medicare Part C and Part D plans. Studies have identified prescription measures that predict future expenditures; however, many are easily manipulable by health plans or practitioners, thus limiting their utility as risk-adjusters. The addition of a non-manipulable prescription utilization measure to existing risk-adjustment models may improve prediction, reducing adverse risk selection incentives by health plans. A secondary objective of this study was to evaluate …

Virtual Worlds And Conservational Channel Evolution And Pollutant Transport Systems (Concepts), Chenchutta Denaye Jackson

Electronic Theses and Dissertations

Many models exist that predict channel morphology. Channel morphology is defined as the change in geometric parameters of a river. Channel morphology is affected by many factors. Some of these factors are caused either by man or by nature. To combat the adverse effects that man and nature may cause to a water system, scientists and engineers develop stream rehabilitation plans. Stream rehabilitation as defined by Shields et al., states that "restoration is the return from a degraded ecosystem back to a close approximation of its remaining natural potential" [Shields et al., 2003]. Engineers construct plans that will restore streams …

Black Men's Responsible Fatherhood Narratives: Fatherhood, Responsibility, Race, And Gender, Shane Chaplin

Electronic Theses and Dissertations

Over the last few decades increasing rates of single mother households in the United States have triggered a national alarm over the effects of father absence on society. Father absence has been linked specifically to many of the problems plaguing black communities in the United States (e.g. poverty, low educational attainment, etc.) and as a result community and political leaders alike have consistently promoted responsible fatherhood practices as a way to address them. Although responsible fatherhood has received, in this context, a considerable amount of social attention, this attention has come intertwined with considerable political and moral rhetoric at all …

Computational Ligand-Based Cns Therapeutic Design: The Search For Novel-Scaffold Norepinephrine Transporter Inhibitors, Anna Chaly

Electronic Theses and Dissertations

Monoamine transporter (MAT) proteins are responsible for regulating cellular signal transduction through control of neurotransmitter reuptake in the synapse, and are therefore relevant to diseases including addiction, psychosis, anxiety and depression. MATs, specifically the serotonin transporter (SERT or 5-HTT), norepinephrine transporter (NET), and dopamine transporter (DAT), serve as the principal targets for antidepressant drugs, such as SSRIs (selective serotonin reuptake inhibitors), NRIs (norepinephrine reuptake inhibitors) and TCAs (tricyclic antidepressants), as well as psychostimulant drugs of abuse such as cocaine and the amphetamines. Due to a lack of crystallographic MAT data, it is unclear as to which of two MAT protein …

The Lived Experience Of Parenting A Child Adopted From An Institution In Eastern Europe, Kimberly A Curry-Lourenco

Electronic Theses and Dissertations

This study sought to explore the lived experience of parenting a child adopted from an institution in Eastern Europe. A hermeneutic, phenomenologic method following the Utrecht School was used. The setting for this study was a southern state on the east coast of the United States. The sample consisted of 11 participants, nine mothers and two fathers, self- identified as adoptive parents to children from institutions in Eastern Europe. Semi-structured interviews, with thematic analysis of transcriptions were conducted. Five major themes were identified: choosing-being chosen, weaving the fabric, the importance of doing, remaining mindful, and looking-in, looking out. Conclusions were …

Enrichment, Characterization And Identification Of Microbial Communities Associated With Unconventional Shale Gas Production Water, J. Lucas Eastham

Electronic Theses and Dissertations

Unconventional natural gas extraction from the Marcellus Shale requires millions of gallons of water to fracture shale and release natural gas from the formation. This process produces water with high levels of total dissolved solids (TDS); and, efforts to recycle these fluids has stimulated microbial growth in produced water. The objective of this study was to analyze the ionic composition of and characterize microorganisms from Marcellus produced water samples. A semi-synthetic culture medium was designed with high TDS to enrich for halophilic microbes, which yielded robust cultures that were able to grow over a wide range of salinities. DNA extracted …

Theory Of Mind, Pragmatic Language, And Social Skills In Male Adolescents With Autism Spectrum Disorders, Gary Koch

Electronic Theses and Dissertations

Autism Spectrum Disorder (ASD) is characterized a core triad of symptoms: impaired social interaction, problems with verbal and nonverbal communication, and unusual, repetitive, or severely limited activities and interests (APA, 2000). Impairments in social development, however, have been considered the most salient and handicapping aspect of ASD and, traditionally, the primary deficit from which the diagnosis results. From a cognitive standpoint, it has been argued that these social impairments in individuals with ASDs arise as a result of deficits in Theory of Mind (ToM) development. The degree to which impairment in ToM corresponds to real-world social-communicative impairments has received little …

Structure Based Ligand Design For Monoamine Transporters And Mitogen Activated Kinase 5, Sankar Manepalli

Electronic Theses and Dissertations

Depression is a major psychological disorder that affects a person's mental and physical abilities. The National Institute of Mental Health (NIMH) classified it as a serious medical illness. It causes huge economic, as well as financial impact on the people, and it is also becoming a major public health issue. Antidepressant drugs are prescribed to mitigate the suffering caused by this disorder. Different generations of antidepressants have been developed with dissimilar mechanisms of action. According to the Center for Disease Control, the usage of antidepressants has skyrocketed by 400 percent increase over 2005- 2008 survey period. This dramatic rise in …

The Effectiveness Of Creating A Sense Of Community In Online Learning With Social Awareness Information, Darren Mariano

Electronic Theses and Dissertations

Online social interactions differ from face to face interactions and lack the non-verbal cues leading a learner to procrastinate, decreased motivation, feelings of isolation and high drop out rates. Existing research illustrates a need for social awareness information in online education, and this research studied the impact of the visual presence of social information on a learner's sense of connectedness and learning using Rovai's (2002) classroom community scale (CCS). Specifically, the study examined if a learners' sense of connectedness is improved with the exposure to others' social awareness information; and if and to what degree learning advanced due to the …

The Federal Workforce Development Program: An Analysis Of Probationers And Characteristics Associated With And Predictive Of Successful Reentry, Kelley Mcnichols

Electronic Theses and Dissertations

With years of research focusing on soaring incarceration rates, the phenomena of prisoner reentry has been largely overlooked. The majority of incarcerated people will return to the community setting. In fact, millions of recently released offenders are on some form of community based supervision. Today, recidivism is a problem that plagues prisoner reentry. In order to address the challenge of prisoner reentry successfully, reentry initiatives have been established in an attempt to change the way corrections is conceptualized. The purpose of this study was to examine characteristics of probationers that are associated with and predictive of successful reentry. The data …

Computational Techniques To Illuminate Secrets Of The Monoamine Transporters, Bonnie A. Merchant

Electronic Theses and Dissertations

The solute carrier family regulates the flow of various substances such as drugs, amino acids, sugars and inorganic ions across the cell membrane. In particular, the monoamine transporters (MATs) are responsible for the regulation of synaptic neurotransmitter levels. Their structures and conformational rearrangements associated with transport remain unsolved. We employed novel computational techniques to identify the binding pocket of cocaine in the dopamine transporter (DAT) and two intracellular pathways for substrate egress in LeuT and DAT. We review possible coarse grained molecular dynamics techniques to extend the temporal scale accessible in simulations of the MATs. Finally, we present the first …

The Effects Of Total Dissolved Solids On Locomotory Behavior And Body Weight Of Streamside Salamanders, And A Baseline Survey Of Salamander Diversity And Abundance, Meghan Pascuzzi

Electronic Theses and Dissertations

Increased levels of total dissolved solids (TDS) in stream habitats are of concern due to salinity as well as the presence of potentially toxic ions. Natural gas drilling in the Marcellus shale could increase TDS in nearby streams. This thesis investigated the effects of water with elevated TDS on the locomotory activity and body weight of the streamside salamander Desmognathus ochrophaeus. Salamanders were exposed to water collected from streams in southwest Pennsylvania with elevated TDS as well as synthetic ion solutions that mimicked the ionic composition found in streams. Chronic, but not acute exposure to solutions of 1000 ppm TDS …

The Role Of Forensic Nurses In Communities Experiencing Environmental Contamination, Wendy Robinson

Electronic Theses and Dissertations

Purpose

The purpose of this study was to provide an understanding of the self-perceived physiological and psychosocial needs of persons living in communities which have been exposed to environmental contamination, and to provide an understanding of how forensic nurses can be utilized in these communities.

Background

This study was conducted to provide an opportunity for forensic nurses to advance their profession by finding ways that they can move beyond their traditional roles. Dixon and Dixon's Integrative Environmental Health Model was the theoretical framework.

Research Design

This cross-sectional triangulated study used quantitative and qualitative methods. The Community Environmental Health and Rights …

The Effect Of Resveratrol On Lipopolysaccharide-Induced Dopaminergic Deficits And Bv-2 Cell Activation, Katherine Marie Rose

Electronic Theses and Dissertations

Neuroinflammation is a common pathology found in patients with Parkinson's disease (PD). PD involves a loss of dopamine (DA) neurons and an increase in activated microglia with subsequent proinflammatory cytokine secretion in the substantia nigra (SN) and striatum. A loss of DA neurons is found in the offspring of animals exposed prenatally to the bacteriotoxin, lipopolysaccharide (LPS) (Ling et al., 2002). Activation of the extracellular regulated kinases, ERK1/2 and ERK5, the downstream targets in the mitogen-activated protein kinase (MAPK) pathway, has been shown to be involved in the dysregulation of the inflammatory process (Cuschieri and Maier, 2005). Consequently, LPS-induced activation …

A Baseline Study Of Chemical Parameters And Microbial Diversity Of Two Streams In The Ten Mile Creek Watershed In Southwestern Pennsylvania, Jennifer Rutter

Electronic Theses and Dissertations

As drilling for natural gas in the Marcellus Shale becomes increasingly prevalent, both human safety and environmental concerns have arisen. The aim of this study was to assemble an environmental baseline to make an accurate assessment of its possible impacts. Water samples and chemical parameters were collected from Bates Fork, a stream with Marcellus Shale drilling activity and Fonner Run, a sister stream with no drilling activity, on a monthly basis beginning in the summer of 2010. Bacterial DNA was also extracted from water collected at each site and then amplified using primers for the variable ribosomal internal transcribed spacer …

Neuropsychological Predictors Of Math Calculation And Reasoning In School-Aged Children, Dana Schneider

Electronic Theses and Dissertations

After multiple reviews of the literature, which documented that multiple cognitive processes may be involved in mathematics ability and disability, Geary (1993) proposed a model that included three subtypes of math disability: Semantic, Procedural, and Visuospatial. A review of the extant literature produced three studies that examined Geary's three subtypes, which provided some support for Geary's model. Given the paucity of research examining Geary's subtypes of math disability, this study aimed to add to the literature by exploring the presence of these three subtypes in a sample of school-aged children. The sample consisted of 60 participants (30 males, 30 females) …

Characterization Of The Hepatitis C Virus Genome Interactions With The Microrna Mir-122: Potential New Therapeutic Targets For Peptide Nucleic Acid Based Strategies, Valerie Schrott

Electronic Theses and Dissertations

Hepatitis C virus (HCV), a positive-sense RNA virus that chronically infects between 2.7 and 3.9 million Americans, is highly mutational, making the HCV infection difficult to treat. Thus, it is of high interest to search for highly conserved therapeutic targets within the HCV genome. Two such sequences are located within the 5' untranslated region (UTR) of HCV, being complementary for the microRNA miR-122, a liver microRNA essential for the production of the infectious virus. The use of peptide nucleic acids (PNAs) as therapeutic agents has become a promising area of study in recent years. In this study, we characterized the …

Cyberbullying And Suicide Among A Sample Of Lgbtq Young Adults, Heather Schwickrath

Electronic Theses and Dissertations

After an extensive literature review, results indicated research has been conducted examining the links between traditional bullying and suicide, lesbian, gay, bisexual, transgendered, and questioning (LGBTQ) identification and cyberbullying, as well as LGBTQ identification and suicide. However, it appears as though there is a dearth of studies examining the link between young adults identifying as LGBTQ who have experienced cyberbullying and the subsequent suicidal behaviors. The following research study attempts to answer the question of whether cyberbullying mediates the relationship between cyberbullying and suicidal behaviors.

Examining Relational Aggression And Conflict Resolution Skills In Overtly Aggressive Non-Caucasian Females, Jessica R Scott

Electronic Theses and Dissertations

In this study, the researcher sought to examine the differences in relational and social aggression for a non-Caucasian adolescent at-risk population across three different groups. Specifically, the study seeks to determine if the Goodwill Girls Curriculum will decrease covert bullying behaviors (i.e., relational and social aggression) in youth who have been removed from their home school districts due to aggressive behaviors including acts of bullying. A secondary aim of this study will be identify the effects of the Goodwill Girls Curriculum on increasing conflict resolution skills within the sample.

The Goodwill Girls curriculum is designed to be implemented alongside a …

Depictions Of Mental Disorder In Mainstream American Film 1988-2010, Catherine A. Sherman

Electronic Theses and Dissertations

The following qualitative research study examined visual and thematic depictions of mental disorder in mainstream American film from 1988 to 2010. The research was an extension of an earlier investigation on portrayals of psychological disability in Hollywood movies (Levers, 1988, 2001). The theoretical and historical grounding for the project included Sander Gilman's (1982) scholarship on madness in the pictorial arts, the history and treatment of mental disorder over the course of time, social constructionism and the media, and research on media depictions of mental illness. The author employed two content analysis instruments (Levers, 1988, 2001) to record the appearance of …