Digital Commons Network^™

Eating In Opposition: Strategies Of Resistance Through Food In The Lives Of Rural Andean And Appalachian Mountain Women, Veronica A. Limeberry

Electronic Theses and Dissertations

This thesis examines ways in which rural mountain women of Andean Peru and southern Appalachia use their lived histories and food knowledge in ways that counter Cartesian epistemologies regarding national and international food systems. Using women’s fiction and cookbooks, this thesis examines how voice and narrative reclaim women’s spaces within food landscapes. Further, this thesis examines women’s non-profits and grassroots organizations to illustrate the ways in which rural mountain women expand upon their lived histories in ways that contribute to tangible solutions to poverty and hunger in rural mountainous communities. The primary objective of this thesis is to recover rural …

Regulation Of C-Reactive Protein Gene Expression And Function, Avinash N. Thirumalai

Electronic Theses and Dissertations

Human C-reactive protein (CRP) is the prototypic acute phase protein whose serum concentration increases rapidly during inflammation. CRP is also associated with atherosclerosis; it is deposited at lesion sites where it may interact with modified lipoproteins. There are 2 major questions regarding CRP: 1. How is the serum concentration of CRP regulated? 2. What are the functions of CRP in atherosclerosis?

Our first aim was to determine the role of the constitutively expressed transcription factor Oct-1 in regulating CRP gene expression. We found that Oct-1 overexpression inhibited (IL-6+IL-1β)- induced CRP gene expression; maximal inhibition required the binding of Oct-1 to …

Biochemical Characterization Of Sbip-470 And Its Role In Sa-Mediated Signaling In Plants, Danda P. Chapagai

Electronic Theses and Dissertations

Salicylic acid binding protein 2 (SABP2) is known to play a key role in Salicylic acid mediated defense pathway. SBIP-470 is SABP2 interacting protein that might be putatively involved in transfer of lipids. SBIP-470 was cloned without the signal peptide and expressed in E. coli. In vitro lipid binding assay using recombinant SBIP-470 failed to detect lipid binding. In vitro lipid transfer assay showed recombinant SBIP-470 does not transfer phospholipid. Study has shown that SBIP-470 is highly inducible upon infection with viral as well as bacterial pathogens. Induction of SBIP-470 expression upon the TMV infection most likely depends upon …

The Use Of Variable-Bagging And The Cross-Validation Selector In The Prediction Of Alzheimer’S Using The Adni Database., Michael Wayne Godbey

Electronic Theses and Dissertations

Dimensionality plays a huge part in the modeling process. If there are more elements in a data set than variables in each element then there are very few restrictions in selection of an algorithm. Bagging, bootstrap aggregating (Breiman, 1994), may also be used to improve a model’s prediction capability. On the other hand, if there more variables in each observation than the number of observations in the dataset, the number of usable algorithms is greatly reduced. The recently developed algorithm, support vector machines, was designed for such situations, in comparison to algorithms such as logistic regression which have instability issues …

Cultural Capital And The Family-School Mesosystem : A Multiple Groups Analysis Of School-Based Parent Involvement Types And Their Relations With Early Student Achievement., Emily R. Dickinson 1974-

Electronic Theses and Dissertations

This dissertation study explored the relationship between school-based parent involvement and early reading outcomes by positing that different types of parent involvement activities reflect access to different forms of cultural capital and therefore should be analyzed as separate constructs. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) techniques were used to establish the factor structure underlying measures of school-based parent involvement available in the Early Childhood Longitudinal Study-Kindergarten Cohort of 2011 (ECLS-K: 2011). Also of interest were the variations in the amount of participation in different types of involvement between families from various sociocultural backgrounds, as well as the …

Pathogenic Role Of Acrolein In Alcoholic Liver Disease., Wei-Yang (Jeremy) Chen

Electronic Theses and Dissertations

Alcohol is the most socially accepted addictive drug, and it can cause alcoholic liver disease (ALD), which is a major cause of morbidity and mortality in the United States and worldwide. Animal and human studies demonstrate that chronic alcohol consumption causes a pro-oxidant environment in the liver and increases hepatic lipid peroxidation and the accumulation of by-products such as acrolein and 4-hydroxynonenal. Acrolein is the most reactive and toxic aldehyde generated through lipid peroxidation. Also, acrolein is a major component of cigarette smoke, and there is increasing evidence that smoking negatively impacts the incidence, severity, and clinical course of chronic …

Pathogenic Role Of Phosphodiesterase 4 (Pde4) In The Development Of Alcohol Induced Hepatic Steatosis., Diana Veronica Avila

Electronic Theses and Dissertations

Alcohol induced hepatic steatosis is a significant risk factor for progressive liver disease. Steatotic hepatocytes have increased sensitivity to injury produced by inflammatory cytokines, particularly TNF. Cyclic adenosine monophosphate (cAMP) has been shown to play a significant role in the regulation of both TNF production and lipid metabolism. However, the role of altered cAMP homeostasis in alcohol mediated hepatic steatosis and injury has not been studied. cAMP levels are tightly regulated by phosphodiesterase family of enzymes. Our recent work demonstrated that increased expression of hepatic PDE4, which specifically hydrolyzes and decreases cAMP levels, plays a pathogenic role in the development …

Prompting Discussion : Writing Prompts, Habits Of Mind, And The Shape Of The Writing Classroom., William Matthew Wiles

Electronic Theses and Dissertations

Much of the important writing students will be tasked with in their college careers calls upon them to approximate the writing styles academics and professionals use to shape and advance their respective fields. Many disciplinary values are encoded within the such texts. Learning these styles can be difficult for students who lack the experience and ingrained habits that their instructors may take for granted. In most cases, college writers are outsiders peeking in from the outskirts of academic and professional discourse. Also, the “Expert Blind Spot” (Ambrose et. al) can make college instructors oblivious to the nuances of the writing …

Novel Analytical Tools For Studies In Molecular Assemblies : I. Electro-Active Single-Mode Integrated Optical Waveguides ; Ii. Coupled Plasmon Waveguide Resonances., Xue Han

Electronic Theses and Dissertations

Two optical analytical tools were developed for studying molecular assemblies at solid/liquid interfaces. Electro-active single-mode integrated optical waveguide (EA-SM-IOW) technology was developed as a platform for spectroelectrochemical investigations on redox adsorbates at the sub-monolayer level. With an optimized ultra-thin indium tin oxide film combined with a single-mode integrated optical waveguide, for the first time, a more than 14,000 times higher sensitivity (compared to conventional potential-modulated transmittance) was achieved. From optical signals, this technique was able to reconstruct electrochemical information of redox adsorbates, including the formal potential and the electron transfer rate. A few major advantages were achieved with the developed …

The Impact Of Inflammatory And Immune Response-Related Genetic Variants On Prostate Cancer Risk Among Men Of African Descent., Dominique Zilpha Jones

Electronic Theses and Dissertations

Background: In the U.S., prostate cancer (PCA) metastasis is associated with a 5-yr survival rate of 29% and disproportionately affects men of African descent. Single nucleotide polymorphisms (SNPs) may serve as genetic markers to identify individuals at high risk of developing PCA. Methods: We examined main and joint effects of 119 inflammatory and immune response-associated SNPs among men of African descent (535 controls, 279 cases) via logistic regression (LR) and entropy-based-multi-factor dimensionality reduction (MDR) modeling. Results: MDR yielded highly synergistic [(TLR10, TLR6 and IRF3), (TLR2, IRAK4), (IL1R2, IL10, IL10RA)] interaction …

Modulation Of The Ovarian Cancer Humoral Response By Tumor-Derived Exosomes., Carolyn Denise Roberson

Electronic Theses and Dissertations

The pathogenesis of epithelial ovarian cancer is complicated by its diagnosis during the latter stages of the disease resulting from lack of symptoms or from presence of symptoms that mimic other conditions. Because of the often fatal prognosis by the time of actual detection, efforts are being made to better understand the host immune response to ovarian cancer. In this study, the contributions of the humoral immune response were investigated by focusing on the role of tumor-derived exosomes and their ability to modulate humoral immune responses. First, ovarian cancer patient-derived free circulating antibodies were investigated for immunoreactivity to patient antigen. …

Fabrication And Characterization Of Antioxidant Loaded Liposomes., Brian Gettler

Electronic Theses and Dissertations

Radiation exposure is both a major obstacle in space exploration and an occupational hazard for various careers causing DNA damage and ROS. In order to reduce the effects of radiation, the primary and most explored approach has been focused around mitigation in terms of exposure duration, exposure intensity, and shielding usage. In some situations none of these reduction strategies were viable and so the use of antioxidants is being explored as an alternate strategy for therapeutic purposes. One such antioxidant is curcumin, otherwise known as diferuloylmethane, is a component of turmeric with both antioxidant properties and anti-inflammatory properties. Another antioxidant …

Fabrication Of A Thin Silver Nanowire Composite Film And Investigation Of A Patterning Technique., Richard S. Korte 1991-

Electronic Theses and Dissertations

Electrically conductive polymers encompass an exciting field of research for applications in dye-sensitized solar cells (DSSC’s). DSSC’s possess several advantages over other types of solar cells. They offer the potential for high quantum efficiency, solar conversion efficiency approaching that of traditional silicon panels, rapid charge transfer kinetics for photo-excited electrons, mechanical flexibility, and cost efficient manufacturing processes. However, key drawbacks to their large scale production and performance lifetime lie in their reliance on costly indium tin oxide (ITO), fluorinated tin oxide (FTO), and platinum for electrode materials, and the mechanical fragility inherent to a liquid electrolyte layer component. Much of …

An Examination Of Successful Socioeconomically Disadvantaged Males In A Northeast Tennessee Middle School, Stephen E. Long

Electronic Theses and Dissertations

The purpose of this qualitative case study was to gain an in-depth understanding of the factors that contribute to positive educational outcomes as measured by the EXPLORE test for eighth grade males who qualify for free or reduced price lunch. In addition, this study was conducted for the purpose of improving the educational program at a Northeast Tennessee middle school. Archival EXPLORE data, as well as free and reduced price lunch data, were used to identify high school graduates, 6 males and 6 females, who had performed at or above expectation on the eighth grade EXPLORE test. Females were included …

Ranking Methods For Global Optimization Of Molecular Structures, John Norman Mcmeen Jr

Electronic Theses and Dissertations

This work presents heuristics for searching large sets of molecular structures for low-energy, stable systems. The goal is to find the globally optimal structures in less time or by consuming less computational resources. The strategies intermittently evaluate and rank structures during molecular dynamics optimizations, culling possible weaker solutions from evaluations earlier, leaving better solutions to receive more simulation time. Although some imprecision was introduced from not allowing all structures to fully optimize before ranking, the strategies identify metrics that can be used to make these searches more efficient when computational resources are limited.

Self-Regulation Theory And Self-Monitoring Of Blood Glucose Behavior In Type 2 Diabetes Mellitus., Jennifer E. F. Ward

Electronic Theses and Dissertations

The present study examined self-monitoring of blood glucose (SMBG) as part of a selfregulatory process of health decision-making using the Self-Regulation Model of illness perceptions, or Common Sense Model. Participants were N=185 individuals with type 2 diabetes from a specialty diabetes clinic prescribed subcutaneous insulin or other injectable diabetes medication at least daily. Collected information included both medical chart data and self-report questionnaires completed prior receiving lab results. Self-care burden was generally high; the modal prescribed times per day of injecting insulin was 4 with modal SMBG recommendations of 3-4 times per day. Participants reported high adherence to prescribed medication …

Biomarker Discovery Of Liver Diseases Using Mass Spectrometry-Based Metabolomics., Xue Shi

Electronic Theses and Dissertations

Metabolomics has emerged as one of the latest of the "-omics" disciplines that can detect metabolite biomarkers in biological samples. The diverse characteristics of metabolites make the analytical platform challenging in metabolomics. Two bioanalytical platforms were developed in this study to investigate metabolite abundance changes under different biological conditions. We first developed a bioanalytical platform that coupled linear trap quadruple - Fourier transform ion cyclotron mass spectrometer (LTQ-FTICR MS) with direct infusion chip-based nano-electrospray ionization (DI-nESI) and applied it to study polychlorinated biphenyls (PCB) effects on non-alcoholic fatty liver disease (NAFLD). We also employed this platform in conjunction with in …

Assessing The Social And Ecological Factors That Influence Childhood Overweight And Obesity, Katie Callahan

Electronic Theses and Dissertations

The prevalence of childhood overweight and obesity is increasing at an alarming rate in the United States. Currently more than 1 in 3 children aged 2-19 are overweight or obese. This is of major concern because childhood overweight and obesity leads to chronic conditions such as type II diabetes and tracks into adulthood, where more severe adverse health outcomes arise. In this study I used the premise of the social ecological model (SEM) to analyze the common levels that a child is exposed to daily; the intrapersonal level, the interpersonal level, the school level, and the community level to better …

The Phenomenon Of Abstract Cognition Among Scholastic Chess Participants: A Case Study, Brent C. Laws

Electronic Theses and Dissertations

A qualitative investigation was conducted to explore the phenomenon of abstract cognition among a purposive sample of 5 secondary scholastic chess club participants. The case study enabled the researcher to explore the faculties of abstract cognition among students of contrasting skills and abilities in playing chess. The study also allowed for the consideration of potential visual-spatial, logical, academic, social competency and life benefits of chess play. Through analysis of interviews, chess simulations, blindfold chess play, and narration of chess lines and sequences, the investigator was able to extract meaning and code schemata into a holistic understanding of the phenomenon of …

Weightlifting, Performing Arts Electives, And Academic Achievement: A Comparison In An East Tennessee High School, Lana Page

Electronic Theses and Dissertations

The purpose of this study was to determine if there was a significant relationship between the academic achievement as measured by ACT English, math, and composite scores of students who took 6 or more credits of weightlifting, 6 or more credits of performing arts, or no elective focus in an East Tennessee high school serving grades 9-12 from the graduating classes of 2010 through 2014. The independent variables were the elective focus groups students took during high school and race and ethnicity. The dependent variables were ACT English, math, and composite scores. A series of one-way analyses of variances (ANOVAs) …

Oxidative Damage To Dna 2´-Deoxyribose By Carbonate Radicals: Reaction Mechanisms And Products, Terence J. Moore

Electronic Theses and Dissertations

The carbonate radical anion (CO₃^•-, CR) is an important reactive oxygen species produced in vivo by one-electron oxidation of CO₂ or bicarbonate, constituents of the major physiological buffer. It was demonstrated for the first time by using an HPLC-based analysis of low-molecular products of DNA damage that CRs react with DNA 2΄-deoxyribose by the hydrogen abstraction mechanism. CRs exhibit a ~ 800-fold preference for one-electron oxidation of guanine over hydrogen abstraction from DNA sugar, in sharp contrast with •OH. CRs also have, as compared to •OH, an increased preference for the H1΄ abstraction, which is the …

“Our Weapon Is The Wooden Spoon:” Motherhood, Racism, And War: The Diverse Roles Of Women In Nazi Germany, Cortney Nelson

Electronic Theses and Dissertations

The historiography of women in Nazi Germany attests to the various roles of women in the Third Reich. Although politically invisible, women were deeply involved in the Nazi regime, whether they supported the Party or not. During Nazi racial schemes, men formed and executed Nazi racial programs, but women participated in Nazi racism as students, nurses, and violent perpetrators. Early studies of German women during World War II focused on the lack of Nazi mobilization of women into the wartime labor force, but many women already held positions in the labor force before the war. Nazi mistreatment of lower-class working …

Mathematical Modeling Of Immune Responses To Hepatitis C Virus Infection, Ivan Ramirez

Electronic Theses and Dissertations

An existing mathematical model of ordinary differential equations was studied to better understand the interactions between hepatitis C virus (HCV) and the immune system cells in the human body. Three possible qualitative scenarios were explored: dominant CTL response, dominant antibody response, and coexistence. Additionally, a sensitivity analysis was carried out to rank model parameters for each of these scenarios. Therapy was addressed as an optimal control problem. Numerical solutions of optimal controls were computed using a forward-backward sweep scheme for each scenario. Model parameters were estimated using ordinary least squares fitting from longitudinal data (serum HCV RNA measurements) given in …

Factors Impacting Success In Ninth Grade Algebra I For High School Students, James M. Lamie

Electronic Theses and Dissertations

The implementation of the Common Core State Standards (CCSS, 2013), No Child Left Behind Act of 2001, and the Race to the Top initiative of 2009 has placed a greater emphasis on high stakes testing. A renewed emphasis on math education for all students and their ability to succeed in high school mathematics place new challenges on today’s classroom teachers. Although the belief is that with new standards and best teaching strategies students will improve their test scores, there are multiple factors that can serve as deterrents to the success in mathematics for at-risk high school students (Balfanz, 2009). The …

Cloning And Expression Of A Tobacco Stearoyl-Acp Desaturase Gene Sbip24 And Its Interaction With Sabp2 In Sa Pathway, Amin J. Ferdous

Electronic Theses and Dissertations

Salicylic acid binding protein 2 (SABP2) that converts methyl salicylate to salicylic acid (SA) plays an obligatory role in the SA-mediated disease resistance pathway in plants. SABP2 interacts with SBIP24 in a yeast two-hybrid screening. SBIP24 belongs to the stearoyl-acyl carrier protein-desaturase protein family. To biochemically characterize the SBIP24, it was cloned from tobacco leaves using RT-PCR and expressed in E. coli. Recombinant SBIP24 was affinity purified using Ni-NTA chromatography. RT-PCR was performed to determine the role of SABP2 in modulating the expression SBIP24. TMV infected transgenic C3 (control tobacco plant containing empty silencing vector) and 1-2 (SABP2-silenced) …

Impulsivity, Venturesomeness, And Pride: Potential Moderators Of The Relationship Between Childhood Trauma, Substance Use, And Physical Aggression, Joshua P. Hatfield

Electronic Theses and Dissertations

Impulsivity, venturesomeness, and pride variables were examined as potential moderators of the associations between childhood trauma and physical aggression, alcohol use and physical aggression, and drug use and physical aggression. Participants (n = 457) were college students recruited from a university in the Southeast. It was hypothesized that childhood trauma, alcohol use, and drug use would be associated with increased scores of physical aggression. In addition, it was hypothesized that impulsivity, venturesomeness, authentic pride, and hubristic pride would moderate these relationships. Linear, multivariate hierarchical regression analyses were used to examine these variables as potential moderators. Hypotheses concerning hubristic pride as …

Brain Based Learning: K-12 Teachers’ Preferred Methods Of Science Instruction, Donna L. Mansy

Electronic Theses and Dissertations

The purpose of this quantitative study was to investigate Brain Based Learning (BBL) techniques in teaching science. Participants included 216 K-12, full-time, regular education teachers from 8 Northeast Tennessee school systems who taught at least 1 science class. Specifically this research was guided by 7 research questions on teachers’ perceptions and practices in teaching science.

Data were collected by a survey that consisted of 82 statements where teachers rated their level of agreement and was distributed online via Survey Monkey. The first portion of my survey included demographic identifiers, teachers’ knowledge of the term BBL, and inquiries regarding science background …

Gender Similarities And Differences In Experiences Of Public School Administrators, Marcia D. Fawver

Electronic Theses and Dissertations

The purpose of study was to examine similarities and differences in experiences between male and female high school principals and district administrators in addition to similarities and differences in leadership style and skills. This was an in-depth study with participants working in the same district and matched with descriptors such as age, similar position, and time served. Therefore the statement of the problem for the present study is what similarities and differences in experiences exist between male and female high school principals and district administrators in addition to similarities and differences in leadership style and skills.

Two male high school …

Cellular-Based Brain Pathology In The Anterior Cingulate Cortex Of Males With Autism Spectrum Disorder, Jessica D. Crawford

Electronic Theses and Dissertations

Autism spectrum disorder (ASD) now affects 1 in 68 children in the United States. Disorders within this spectrum share hallmark deficits in verbal and nonverbal communication, repetitive behavior, and social interaction. The cause of ASD is still unknown. Even though hundreds of genetic abnormalities have been identified in ASD, these markers account for less than 1% of all ASD cases. Researchers continue to search for pathological markers common to all or most cases of ASD. The research presented in this dissertation used a novel combination of state-of-the-art methods to investigate brain pathology in ASD. Postmortem anterior cingulate cortex (ACC) from …

Hif-1Α In The Heart: Provision Of Ischemic Cardioprotection And Remodeling Of Nucleotide Metabolism, Joe Wu

Electronic Theses and Dissertations

In our studies we found that stabilized expression of HIF-1α in heart led to better recovery of function and less tissue death after 30 minutes of global ischemia, via mechanisms that preserve the mitochondrial polarization. Our group previously showed that HIF-1α conferred ischemic tolerance by allowing cardiomyocytes to use fumarate as an alternative terminal electron acceptor to sustain anaerobic mitochondrial polarization. The source of fumarate was identified as the purine nucleotide cycle (PNC). Here we discovered that HIF-1α upregulates AMP deaminase 2 (AMPD2), the entry point to the PNC. The combination of glycolysis and the PNC may protect the heart's …