Digital Commons Network^™

Conductive Inks And Films Via Intense Pulsed Light., Gabriel L. Draper

Electronic Theses and Dissertations

This research focuses on the investigation of Earth abundant copper and carbon based nanomaterials that are subjected to Intense Pulsed Light Processing to create conductive films, as future flexible electronics and renewable energy solutions would benefit from the quick and scalable production of conductive films. Use of nanomaterials in their oxide/hydroxide forms leads to higher stability in aqueous inks for efficient large area solution deposition. IPL Processing utilized 2044 μs pulses ranging from 589 J - 2070 J over an area of 1.9 cm x 30.5 cm, with energy densities of 10.1, 12.8, 15.8, 19.2, 22.9, 26.8, 31.1 and 35.7 …

Computational Methods For The Analysis Of Functional 4d-Ct Chest Images., Ahmed Soliman Naeem Soliman

Electronic Theses and Dissertations

Medical imaging is an important emerging technology that has been intensively used in the last few decades for disease diagnosis and monitoring as well as for the assessment of treatment effectiveness. Medical images provide a very large amount of valuable information that is too huge to be exploited by radiologists and physicians. Therefore, the design of computer-aided diagnostic (CAD) system, which can be used as an assistive tool for the medical community, is of a great importance. This dissertation deals with the development of a complete CAD system for lung cancer patients, which remains the leading cause of cancer-related death …

Transient Receptor Potential Cation Channel, Subfamilies V, Member 1 (Trpv1) And M, Member 1 (Trpm1) Contribute To Neural Signaling In Mouse Retina., Jennifer Noel

Electronic Theses and Dissertations

The retina processes light information through parallel pathways in order to extract and encode the visual scene. Light information is transmitted to the brain through approximately 30 ganglion cells (GCs), the retinal output neurons. Trp channels modulate the responses of retinal neurons within specific pathways. The study of the expression and function of the majority of Trp channels in the retina is largely in its infancy. My dissertation first investigated the expression and function of the transient receptor potential vanilloid-1 (TRPV1) receptor/channel in the retina. TRPV1, the first cloned and most highly studied Trp channel in the peripheral nervous system, …

Examination Of The Relevance Of Pi3k And Pten Pathway In Ustilago Maydis., Nathaniel Edward Dalton

Electronic Theses and Dissertations

Ustilago maydis is a fungal pathogen that is an obligate parasite on Zea mays (Corn) in order to undergo and complete its sexual life cycle. The cells grow as a haploid form until they meet a compatible partner and undergo a dimorphic switch from a budding yeast-like cell type to a filamentous dikaryon. Infection of the host plant is characterized by leaf chlorosis, anthocyanin content, and formation of large galls that develop on the stem, leaves, cobs, and flowers. Many signaling cascades such as MAPK and PKA are involved in the regulation of the infection lifecycle. The Phosphatidylinosotiol 3 Kinase/ …

Identification And Characterization Of Genes Involved In Metabolism Of N5 Monoene Precursors To N5 Anacardic Acids In The Trichomes Of Pelargonium X Hortorum., Richa A. Singhal

Electronic Theses and Dissertations

Unusual monoenoic fatty acids (UMFA’s) and specialized metabolites called anacardic acids (AnAc) are produced in glandular trichomes of Pelargonium ´ hortorum (geranium). The UMFA’s, 16:1^∆11 and 18:1^∆13 are precursors for the synthesis of unsaturated AnAc 22:1n5and 24:1n5 that contribute to pest resistance in geraniums. UMFAs and their derived AnAc metabolites not only provide a useful biological marker that differentiates the biosynthetic pathway for unusual mononenes from the common fatty acids (i.e. stearic, palmitic, oleic, linoleic and linolenic) but also have industrial, medical and agricultural applications. Fatty acid biosynthesis enzymes like acyl carrier proteins (ACPs); thioesterases (TEs) and β-ketoacyl-ACP …

Retinoic Acid Signaling Regulates Krt5 Independently Of Stem Cell Markers In Submandibular Salivary Gland Epithelium., Timur Maratovich Abashev

Electronic Theses and Dissertations

Vitamin A metabolism, which produces the signaling molecule Retinoic Acid (RA), has been demonstrated to be important for growth and branching morphogenesis of mammalian embryonic salivary gland epithelium. However, it is not known whether RA functions directly within epithelial cells or in associated tissues that influence morphogenesis of salivary epithelium. Moreover, downstream targets of RA transcriptional regulation have not been identified. Here we show that canonical RA signaling occurs in multiple tissues of embryonic mouse salivary glands, including epithelium, associated parasympathetic ganglion neurons, and non‑neuronal mesenchyme. By culturing epithelium explants in isolation from other tissues we demonstrate that RA influences …

Mechanism Of Interaction Of Peptide Modified Nanoparticles With Porphyromonas Gingivalis., Ankita Jain

Electronic Theses and Dissertations

Studies suggest that P. gingivalis functions as a keystone pathogen and interacts with primary colonizers in the supragingival biofilm such as S. gordonii. This interaction contributes to the initial colonization of the oral cavity by P. gingivalis and thus represents a potential target for therapeutic intervention. We have identified a peptide (BAR) derived from the streptococcal SspB protein that functions to inhibit P. gingivalis adherence to S. gordonii. In addition, we showed that nanoparticles (NPs) functionalized with BAR inhibit this interaction more potently than free soluble peptide, possibly by promoting interaction with P. gingivalis at higher valency than …

The Role And Regulation Of Inhibitor Of Kappa B Kinase In The Impaired Monocyte Response., Norman James Galbraith

Electronic Theses and Dissertations

Trauma, major surgery and systemic infection can lead to a subsequent immunosuppressed state which can predispose patients to nosocomial infection and death. Approximately a sixth of patients will have impaired monocyte function, as determined by decreased HLA-DR expression or decreased TNF-α production in response to ex-vivo lipopolysaccharide (LPS) stimulation. This project aimed to develop an in-vitro model of impaired monocyte function using isolated monocytes from healthy volunteers, to study the effect of monocyte impairment on IκK signaling, and determine the role of IκK in the impaired monocyte response. These studies have provided the following results:

1. Monocytes treated with low dose …

Approaches To Arthropod Conservation : Landscape Genetics, Community Assessment, And Prediction Of Extinction Risk., Victoria Annette Prescott

Electronic Theses and Dissertations

Although urbanization is a leading cause of species extinction throughout the world, the impact of urban development on arthropods is little studied and, as a result, poorly understood. I used three distinct approaches to studying arthropod conservation in North America. First, I used landscape genetics techniques to study the impact of urbanization on gene flow among populations of Rabidosa rabida, the rabid wolf spider. While gene flow was not detrimentally reduced, urban development correlated with a reduction in migration rates among populations, and to my knowledge, this is the first study to document isolation by resistance in spiders. Next, …

Detecting Evidence Of Systemic Inflammation From Osteological Markers In The Indian Knoll Population Of Ohio County, Kentucky., Krysta Wilham

Electronic Theses and Dissertations

Indian Knoll is an Archaic shell midden site located in the Green River region of west-central Kentucky, and was excavated twice in the first half of the 20^th century. While Indian Knoll has been the subject of frequent bioarchaeological studies, the present study presents an osteological analysis of the relationships between skeletal markers commonly associated with local inflammatory processes, and that have the potential to be used as proxies for systemic inflammation. The analysis revealed a significant positive association in the presence of periodontal disease (PD) and periosteal lesions (PL), suggesting a potential underlying hyper-inflammatory status or phenotype. These …

Spreading Speeds Along Shifting Resource Gradients In Reaction-Diffusion Models And Lattice Differential Equations., Jin Shang

Electronic Theses and Dissertations

A reaction-diffusion model and a lattice differential equation are introduced to describe the persistence and spread of a species along a shifting habitat gradient. The species is assumed to grow everywhere in space and its growth rate is assumed to be monotone and positive along the habitat region. We show that the persistence and spreading dynamics of a species are dependent on the speed of the shifting edge of the favorable habitat, c, as well as c^*(∞) and c^*(−∞), which are formulated in terms of the dispersal kernel and species growth rates in both directions. When …

The Role Of Nicotine, A7 Nicotinic Acetylcholine Receptors And Extracellular Matrix Remodeling In Pulmonary Fibrosis., Glenn Ward Vicary

Electronic Theses and Dissertations

The median survival for idiopathic pulmonary fibrosis (IPF) patients from diagnosis is a dismal 3 years. This condition is characterized by pulmonary fibroproliferation and excess production and disordered deposition of extracellular matrix (ECM) proteins resulting in obliteration of the original tissue architecture, loss of lung function and eventual death due to respiratory failure. The main hindrance to the development of effective treatments against pulmonary fibrosis is the late detection of its progression and is often of unknown cause. Tobacco smoke represents the most important environmental factor linked to the development of pulmonary fibrosis, with over 60% of IPF patients current …

Assessing Moral Injury And Existential Well-Being In Returning Servicemembers Using The Spiritual Attitudes Inventory - Revised (2010)., Leann E. Armes-Bruce

Electronic Theses and Dissertations

Military personnel returning from deployment often report a variety of distressing symptoms stemming from exposure to prolonged, extreme deployment stress, and traumatic experiences. In addition to reporting symptoms consistent with the diagnosis of Post-Traumatic Stress Disorder, they are also known to report struggling with existential angst often leading to great despair and a sense of profound changes in the core of their being. While this phenomenon, referred to herein as Moral Injury, has traditionally received little attention, it is beginning to emerge as a potentially viable construct in practice and research and may assist in explaining the effects of war …

Dietary Resource Utilization Patterns And Head Morphology Among Three Sympatric Watersnake Species., Micah Warren Perkins

Electronic Theses and Dissertations

The coexistence of similar species may be related to a variety of resource utilization differences. Dietary resource utilization variation may be the most important difference allowing for the coexistence of sympatric snake species. Many watersnakes (Nerodia spp.) live in sympatry and use similar aquatic habitats feeding on similar prey. While these sympatric watersnakes may have different general foraging patterns, snake diet may be affected by a variety of factors. Therefore, I initiated an investigation to understand the coexistence of sympatric plain-bellied (N. erythrogaster), diamondback (N. rhombifer) and northern (N. sipedon) watersnakes by addressing …

Defying Convention: Atypical Perspectives Of Slavery In Antebellum New Orleans, Amanda N. Carr

Electronic Theses and Dissertations

During the first half of the nineteenth century, slavery became a vital economic component upon which the success of the southern states in America rested. Cotton was king, and slavery was the peculiar institution that ensured its dominance in the domestic and international markets of America. Popular portrayals, however, often neglect the complicated dynamics of American slavery and instead depict the institution in simplistic terms. The traditional view has emphasized an image of white southerners as slaveholders and blacks as slaves. In New Orleans, the lives of three men—all of whom were tied to slavery in varying capacities—reveal a much …

Residual Feed Intake And The Relationship With Performance, Temperament And Carcass Traits In Growing And Finishing Steers, Courtney R. Branton

Electronic Theses and Dissertations

Two trials were conducted with Santa Gertrudis steers, trial 1 (n = 115) and trial 2 (n = 118), were fed a roughage-based diet during the growing phase and high-grain diet during the finishing phase. Steers were weighed at 14-d intervals and dry matter intake (DMI) measured for 70 d during both phases. Residual feed intake (RFI) is the difference between actual DMI and DMI predicted from linear regression of DMI on mid-test metabolic body weight. Ultrasound carcass measurements were measured on day 0 and 70 of the growing phase. Steers were harvested after finishing phase and carcass cooler traits …

Using A Mouse Model To Investigate Male Bias In Autism Spectrum Disorder Incidence., Elizabeth Ann Gordon

Electronic Theses and Dissertations

The review (Chapter 1) overviews our current understanding of the sex bias in autism spectrum disorders (ASD), whereby males are 4 times more likely to develop the condition. The longstanding uncertainty surrounding the etiology of autism can be informed by inclusion of both sexes in future research to better understand the mechanism responsible for this sex bias. Both clinical studies and animal models are useful tools to elucidate the underlying cause(s) of sexual dimorphic morphological, physiological, and behavioral differences in the ASD phenotype. Sex differences in social behaviors are common across species, underscoring the importance of assessing baseline sex differences …

Plant Community Responses To The Removal Of Lonicera Maackii From An Urban Woodland Park., Elihu H. Levine

Electronic Theses and Dissertations

Impacts of Lonicera maackii on native forest communities have been widely researched, but long-term responses of plant communities to the removal of this exotic shrub have not been extensively evaluated. The Louisville Olmsted Parks Conservancy removes exotic shrubs and vines to restore ecological processes and native species in the woodlands of Cherokee Park. Paired-plots were established in 2008 to gather baseline herb, vine, tree, and shrub community data. Honeysuckle was removed from one plot of each pair in 2009 and community data were gathered again in 2013. Native herb cover and richness, vine cover, and tree sapling abundance increased where …

Immune Monitoring In Recipients Of Combined Living Donor Kidney And Hematopoietic Stem/Facilitating Cell Transplants., Mark Dewayne Badder

Electronic Theses and Dissertations

Solid organ transplantation coupled with a hematopoietic stem cell transplant from the organ donor allows for the recipient to cease immunosuppressive therapy after transplant via a chimeric immune system. This beneficial effect of stem cell transplants is negatively affected by graft versus host disease (GVHD). Better understanding of the donor and recipient’s immune system is vital to mitigating graft versus host disease and induction of donor chimerism without GVHD. In this study, flow cytometry was used to characterize immune cells of the recipients’ before and up to eighteen months post transplantation. The recipients were categorized into their respective chimeric groups. …

Pro-Inflammatory Cytokines Promote Viral Replication And Cell Cycle Mediators In Arenavirus-Induced Hepatitis., Gretchen E. Holz

Electronic Theses and Dissertations

Lassa virus (LASV) is an arenavirus and causative agent of Lassa fever (LF), a viral hemorrhagic fever in West Africa for which there is no vaccine. Lymphocytic choriomeningitis virus (LCMV), is used as a surrogate to mimic LASV-induced liver pathology. LCMV-WE, not LCMV-ARM, causes disease in primates and mice characterized by hepatitis, high viral load, hepatocyte proliferation, and upregulated proliferative triggers (e.g. TNF-α, IL-6 ). We hypothesize LCMV-WE induces pathological hepatocyte proliferation via pro-inflammatory triggers (TNF-α, IL-6) from macrophages, leading to: increased viral replication, modulated cell cycle, and arrested cell cycle. RAW 264.7 macrophages and AML-12 hepatocytes, were used as …

Establishing A Clinically Relevant Mouse Model Of Human Aml To Test Novel Transmethylation Inhibitors., Aditya Barve

Electronic Theses and Dissertations

Acute myeloid leukemia (AML) is a highly heterogeneous clonal disorder characterized by an accumulation of malignant immature myeloid progenitors in the bone marrow (BM) that hinder normal hematopoiesis. Patient AML exhibits a dramatic heterogeneity in terms of cytogenetics, disease morphology, and associated prognoses and/or chemotherapeutic sensitivity. Thus it becomes clearly evident that the investigation of novel therapeutics for AML will require model systems that are capable of recapitulating this stark heterogeneity in a patient specific manner. Furthermore, it is now understood that the surrounding bone marrow (BM) microenvironment and supporting cells play a critical role in leukemic progression as well …

The Influence Of Student, Neighborhood, And School Factors On College Readiness : An Examination Of Factors Combined As A Result Of Implementing A Race- And Socioeconomically-Based Student Assignment Plan., Gregory Paul Herberger

Electronic Theses and Dissertations

In 2006, Meredith v. Jefferson County Board of Education determined race-based student assignment plans violated the 14th Amendment. Through the assistance of Orfield and Frankenberg (2011), Jefferson County Public Schools (JCPS) reconfigured the district’s race-based student assignment and implemented a race- and socioeconomic-based student assignment plan. The purpose of this study was to examine students’ backgrounds and school composition factors within a race- and socioeconomic-based assignment plan to determine the extent the factors are related to college readiness. Based on data obtained from 3,018 Grade 11 students, hierarchical linear multiple regression was used to examine the utility of student background, …

Tnf-Like Weak Inducer Of Apoptosis (Tweak) : Not So Weak After All., Joseph Dekward Mcmillan Iv

Electronic Theses and Dissertations

Background: Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) is a proinflammatory cytokine belonging to the TNF super family. TWEAK produces a variety of cellular responses through the binding to fibroblast growth factor inducible 14 (Fn14), a member of TNF receptor superfamily. Although Fn14 lacks a death domain, TWEAK has been found to induce apoptosis in some cell types by perturbing the activity of certain pathways such as TNF-receptor signaling. TWEAK is also known to regulate proliferation and differentiation of myogenic cells. We have previously reported that the TWEAK-Fn14 system causes skeletal muscle wasting both in vitro and in …

Glutathione S-Transferase P In Glucose Homeostasis In Mice., Shubha Ghosh Dastidar

Electronic Theses and Dissertations

High calorie diets have fostered the current pandemic of obesity and comorbid conditions of non-alcoholic fatty liver disease (NAFLD), systemic insulin resistance (IR) and type 2 diabetes (T2D). Hepatic glutathione S-transferases (GSTs) are dysregulated in obesity, NAFLD and diabetes. The multifunctional GST pi isoform (GSTP) catalyzes acrolein metabolism and inhibits JNK (c-Jun NH₂-terminal kinase). The purpose of this study was to test specifically whether GSTP deficiency disturbs glucose homeostasis in mice. Hepatic GST proteins were downregulated by short-term high fat diet (HFD) in wild type (WT) mice concomitant with glucose intolerance, increased hepatic JNK activation and protein-acrolein …

The Role Of The Mir-200 Family On The Tumor Suppressor Rassf2 And The Effect On Mapk Pathway Activity In Colorectal Cancer., Jane V. Carter

Electronic Theses and Dissertations

This dissertation investigated the role of the miR-200 family in normal colon epithelial (CCD 841) and Dukes’ C (HT-29) colorectal cancer (CRC) cell lines. Our aim was to characterize expression of the miR-200 family (miR-200a, miR-200b, miR-200c, miR-141, and miR-429) in colorectal cell lines, study their effect on the tumor suppressor Ras Associated Domain-Containing Protein (RASSF) 2 and on subsequent activity within the mitogen-activated protein kinase (MAPK) signaling pathway. We wanted to determine whether regulation of miR-200 family members could change cell behavior towards more “cancer-like” in a normal colon epithelium (CCD 841) cell line, or less “cancer-like” in a …

"If This Stuff Matters, Why Isn't It Being Shared?" : Citations, Hyperlinks, And Potential Public Futures Of Online Writing In Rhetoric And Composition., Elizabeth Frances Bergeron Chamberlain

Electronic Theses and Dissertations

This dissertation addresses two deceptively discrete questions: (1) how academics might reach wider public audiences, and (2) how and why people cite the way they do. It takes citation practices as a telling though often tacit practice, one through which it is possible trace the contours of a larger story about how writing is changing as it moves online. That story: Writers increasingly reflect goals of provocation, of attracting a wider and potentially global audience, of spreading a message rapidly and virally, of responding to recent events and conversations, of sharing sources and resources. To explore these questions, this dissertation …

Distress, Circadian Rhythms, Immunity, And Survival In Presurgical Breast Cancer Patients., Whitney Nicole Rebholz

Electronic Theses and Dissertations

Women with breast cancer experience significant stress related to diagnosis and treatment, which may negatively affect physiology (e.g., circadian, endocrine, and immune) and contribute to cancer progression. Demographic (e.g., ethnic minority) and neighborhood factors can contribute to cancer-related stress. African American women with breast cancer experience more adverse disease pathology and earlier mortality; however, factors that explain these disparities rarely have been explored. It is hypothesized that psychosocial stressors (i.e., ethnicity, neighborhood stress, and cancerrelated stress) will be related to one another. Associations of psychosocial stress with physiological disruption are posited with regard to circadian, endocrine, and immune function. It …

Syntheses And Characterization Of A New Cyano-Substituted Bis(Pyrazolyl)Borate And Its Thallium (I) Complex, Chris Acquah

Electronic Theses and Dissertations

Scorpionates are versatile and flexible ligands with a wide range of applications including catalysis, C-H bond activation, formation of new class of materials, and mimicking enzymatic reactions. This is as a result of its steric and electronic properties, and due to the relative ease with which the 3, and 5-positions of the pyrazole ring can be functionalized. In this work, we report the synthesis of a new class of scorpionate ligands known as cyanoscorpionates which can crosslink various metal centers. Thus, bis (4-cyano-3,5-diphenylpyrazolyl)borate Bp^Ph2,4CN was synthesized and fully characterized by ¹H NMR and FT-IR. Coordination of bis (4-cyano-3,5-diphenylpyrazolyl)borate …

Portrayal Of African Women In Nollywood Films Over A Five-Year Period: A Content Analysis Of Traits Applying The Stereotype Content Model, Olushola Aromona

Electronic Theses and Dissertations

Previous research shows that perceptions, attitudes, and beliefs are cultivated via media exposure. A content analysis of stereotypical traits in five Nollywood movies in a five-year span examines the prevalent portrayals of women in the Nigerian movie industry – Nollywood, and the effect of these portrayals on reinforcement of stereotypical norms and perpetuation of gender disparity. Cultivation and Objectification theories were the theoretical frameworks for this study. Findings revealed no significant change in the stereotypical portrayals of women in the past five years. From the movies analyzed, Nollywood movies appear to remain persistent in typically depicting women as unambitious domestic …

Pharmacokinetics, Tissue Distribution, Synergistic Activity, And Antitumor Activity Of Two Isomeric Flavones, Crystal L. Whitted

Electronic Theses and Dissertations

Flavonoids are polyphenolic secondary metabolites found in plants that have bioactive properties including antiviral, antioxidant, and anticancer. Two isomeric flavone were extracted from Gnaphalium elegans and Achyrocline bogotensis, plants used by the people from the Andean region of South America as remedies for cancer. 5,7-dihydroxy-3,6,8-trimethoxy-2-phenyl-4H-chromen-4-one (5, 7–dihydroxy- 3, 6, 8 trimethoxy flavone/ flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy-2-phenyl-4H-chromen-4-one (3, 5–dihydroxy-6, 7, 8–trimethoxy flavone/ flavone B) have shown antineoplastic activity against colon cancer cell lines dependent upon their differentiation status. Pharmacokinetic studies reported herein were used to determine dosing for antitumor assays, as well as determine target tissue concentration. These included the …